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The Diagnosis of Fetal Sexing in Cattle Using Ultrasound 超声诊断牛胎儿性别的研究
L. Bogdan, A. Petrean, I. Coman, G. Nadăș, M. Cenariu, I. Bogdan, S. Bogdan, D. Berean
The purpose of this research was to conduct an ultrasound exam in the interval between 49-120 days of gestation for the determination of fetus sex and to establish the interval when the fetal sexing is possible. The research was carried out in three farms from Transylvania. In farm A were examined 25 animals, in farm B 13 animals and in farm C 11 animals. The diagnosis of the fetal sexing was possible for 35 cases, 14 animals were diagnosed as female and 21 were male. In the interval between 56-65 days of gestation the diagnosis of fetal sexing was established by viewing the genital tubercle and in the interval between 65-90 days of gestation the diagnosis was established by viewing the secondary genital organs. In 14 cases the diagnosis was not set; in 4 cases the conception product has not been sufficiently developed, the genital tubercle was not visible, and in 10 cases the fetus was too big and was impossible to localize the genital organs.
本研究的目的是在妊娠49-120天之间进行超声检查,以确定胎儿性别,并确定胎儿性别可能的时间间隔。这项研究是在特兰西瓦尼亚的三个农场进行的。A农场检查了25只动物,B农场检查了13只动物,C农场检查了11只动物。35例可诊断胎儿性别,其中14例为雌性,21例为雄性。在妊娠56-65天期间,通过观察生殖器结节来诊断胎儿性别,在妊娠65-90天期间,通过观察第二生殖器官来诊断胎儿性别。14例未确诊;4例受精卵发育不充分,未见生殖结节,10例胎儿过大,无法定位生殖器官。
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引用次数: 0
In Vivo and In Vitro Pharmacodynamics of Anthelmintic Medication Used in Sheep 绵羊驱虫药的体内外药效学研究
M. Cernea, R. Filip, R. Catana, L. Cătană
Our research aimed to evaluate the efficacy of anthelmintic treatments in sheep, as well as the resistance occurrence risk for the most commonly used substances. Anthelmintic medication efficacy was evaluated on 30 animals from a private farm, located in Sânmihaiu Almaşului, Sălaj County. In vivo studies were performed by using Fecal Egg Count Reduction Test (FECRT) and testing an albendazole-based (ABZ) product. In vitro, we used Egg Hatch Assay (EHA) and Larval Development Assay (LDA) for albendazole (ABZ), mebendazole (MBZ), fenbendazole (FBZ), thiabendazole (TBZ) and ivermectin (IVM) (only for LDA). FECRT showed that intestinal nematodes developed resistance phenomena against the ABZ-based product, with an extensivity of 80% at seven days post therapy, an egg reduction percentage of 41.89% at seven days post-therapy and 43.9% at 14 days post-therapy. The in vitro EHA highlighted a superior efficacy of TBZ (egg hatch percentage at reference concentration being 51.21) compared to ABZ (71.89%), MBZ (84.46%) and FBZ (79.22%), with a minimum risk of anthelmintic resistance. The LDA test revealed the superior efficacy of FBZ (MIC 0.59 mcg/ml) and IVM (MIC 0.078 mcg/ml), with a minimal risk of inducing parasitic resistance. All in vivo and in vitro tests revealed a limited ABZ efficacy, recommending avoiding the therapy with this substance.
我们的研究旨在评估绵羊的驱虫药治疗效果,以及对最常用物质的抗性发生风险。本研究对来自苏里拉吉县s nmihaiu alma ului一家私人农场的30只动物进行了驱虫药物疗效评估。体内研究采用粪卵计数减少试验(FECRT)和阿苯达唑(ABZ)产品进行。体外分别对阿苯达唑(ABZ)、甲苯达唑(MBZ)、非苯达唑(FBZ)、噻苯达唑(TBZ)和伊维菌素(IVM)进行卵孵化试验(EHA)和幼虫发育试验(LDA)。FECRT结果显示,肠道线虫对abz产品产生耐药现象,治疗后第7天的耐药率为80%,治疗后第7天和第14天的减卵率分别为41.89%和43.9%。体外EHA试验结果表明,TBZ的卵孵化率为51.21%,优于ABZ(71.89%)、MBZ(84.46%)和FBZ(79.22%),且对虫虫产生抗性的风险最小。LDA试验结果显示,FBZ (MIC为0.59 mcg/ml)和IVM (MIC为0.078 mcg/ml)药效显著,诱导寄生抗性风险最小。所有体内和体外试验显示有限的ABZ功效,建议避免使用该物质治疗。
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引用次数: 0
Gastrointestinal Parasites of Free-Range Chickens – A Worldwide Issue 散养鸡的胃肠道寄生虫——一个世界性的问题
J. Lozano, A. Ana, Antonio Palomero Salinero, E. Hoppe, Lídia Gomes, A. Paz-Silva, M. Rebelo, L. M. Carvalho
Gastrointestinal parasites with direct and/or indirect life cycles, namely Eimeria spp., Ascaridia galli, Heterakis gallinarum and Capillaria spp., are responsible for the most frequent and heavy economic losses in aviculture. The current review aimed to collect information about gastrointestinal parasites affecting poultry, as well as, research studies regarding parasitic diseases in free-range chicken production. From the references used in this review, 20% highlight research studies correlating prevalence of gastrointestinal parasites in free-range chickens and factors such as the production system, age and animal density, hygienic conditions and weather.Coccidiosis is responsible for the major economic losses in poultry farms. Diseases have different clinical signs and result in variable impacts on animals. Control programs include mainly vaccination, anti-coccidia and anthelminthic drugs, as well as, house cleaning and disinfection. This review concluded that coccidia and helminths are a global threat to free-range poultry production. More research in this area is necessary in order to understand the major factors influencing the prevalence of parasitic diseases in this type of poultry production. Also the search for new control strategies must be a priority, mainly using natural antiparasitic compounds and biological control approaches.
具有直接和/或间接生命周期的胃肠道寄生虫,即艾美耳球虫、鸡蛔虫、鸡异毛虫和毛细毛虫,是造成养鸡业最频繁和最严重的经济损失的原因。本综述旨在收集影响家禽的胃肠道寄生虫的信息,以及关于散养鸡生产中寄生虫病的研究。在本综述中使用的参考文献中,20%强调了将散养鸡胃肠道寄生虫患病率与生产系统、年龄和动物密度、卫生条件和天气等因素相关的研究。球虫病是造成家禽养殖场主要经济损失的原因。疾病有不同的临床症状,对动物的影响也不同。控制方案主要包括疫苗接种,抗球虫和驱虫药,以及房屋清洁和消毒。本综述认为球虫和蠕虫是对散养家禽生产的全球性威胁。为了了解影响这类家禽生产中寄生虫病流行的主要因素,有必要在这一领域进行更多的研究。此外,必须优先寻找新的控制策略,主要使用天然抗寄生虫化合物和生物控制方法。
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引用次数: 9
Monitoring of Mycotoxins in Feed for Dairy Cows and Swine from Muntenia Region, Romania 罗马尼亚蒙泰尼亚地区奶牛和猪饲料中真菌毒素的监测
A. Marina, M. Mihaiu, L. Colobatiu, R. Mihaiu, R. Sobolu, A. Cioca
Mycotoxins have a strong negative impact on crops, animal health and human health. These secondary metabolites are toxic can cause major economic losses and illnesses (Zain, 2011). This study aimed to evaluate the presence of the most prevalent mycotoxins, aflatoxin B1 (AFB1), ochratoxin A (OTA), deoxynivalenol (DON) and zearalenone (ZEN), in feed intended for dairy cows and swine from farms located in the Region of Muntenia (Călăraşi, Giurgiu and Teleorman), Romania. 136 samples of fodder and forage intended for cattle and 37 samples for pigs were collected during 2017 2018. Some of the samples were analyzed within the national surveillance program and some on request. AFB1 has been extracted and purified in an immunoaffinity column specific for AFB1. Detection and quantification of toxic secondary metabolites were performed by high-performance liquid chromatography (HPLC) and Enzyme-Linked ImmunoSorbent Assay (ELISA). All four mycotoxins were detectable and quantifiable. The level of contamination was generally low. The maximum allowable limits for AFB1, OTA, DON and ZEN were not exceeded. The low level of contamination indicates no potential risks for animal and human health and suggests that the best practices in the management and storage of feed were applied in the studied area.
真菌毒素对作物、动物健康和人类健康具有强烈的负面影响。这些次生代谢物是有毒的,会造成重大的经济损失和疾病(Zain, 2011)。本研究旨在评估产自罗马尼亚Muntenia地区(cunilra、Giurgiu和Teleorman)农场的奶牛和猪饲料中最常见的真菌毒素黄曲霉毒素B1 (AFB1)、赭曲霉毒素A (OTA)、脱氧雪腐菌醇(DON)和玉米赤霉烯酮(ZEN)的存在情况。在2017 - 2018年期间,收集了136份牛饲料和饲料样本和37份猪饲料样本。一些样本是在国家监测计划范围内分析的,一些是应要求分析的。AFB1在AFB1特异性的免疫亲和柱中被提取和纯化。采用高效液相色谱法(HPLC)和酶联免疫吸附法(ELISA)对毒副代谢产物进行检测和定量。所有四种真菌毒素均可检测和量化。污染程度普遍较低。AFB1、OTA、DON和ZEN均未超过最大允许限量。低污染水平表明对动物和人类健康没有潜在风险,并表明在研究地区采用了饲料管理和储存的最佳做法。
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引用次数: 0
Evaluation of Rectal and Skin Temperature Variations of Anesthetised Dogs Undergoing Magnetic Resonance Imaging Diagnosis 磁共振成像诊断麻醉犬直肠和皮肤温度变化的评价
A. Degan, R. Tudor, R. Costea, D. Bîrțoiu, Mihai Săvescu, A. Șonea
General anesthesia produces different degrees of central nervous depression and changes in the peripheral circulation, therefore affecting the patient’s thermoregulatory mechanism. Moreover, the lack of proper, specially designed equipment for magnetic resonance imaging (MRI) environment monitoring can represent a challenge for the anesthetist. We examined the temperature variations correlated with different anesthetic protocols in dogs that underwent general anesthesia in order to evaluate changes in rectal and distal extremities temperature, before and after anesthesia. This study was conducted at the Faculty of Veterinary Medicine in Bucharest, on 21 dogs that were divided in 3 groups depending on the anesthetic protocol used. First group (B) received butorphanol (0.2 mg/kg, intravenously IV), second group (BK) had butorphanol (0.2 mg/kg) and a low dose of ketamine (2 mg/kg) IV, and group 3 (BM) was premedicated with butorphanol (0.2 mg/kg) and midazolam (0.2 mg/kg) IV. All patients were induced with propofol i.v. (3.24±0.68), intubated and maintained with isoflurane and oxygen. We determined rectal temperature before and right after the end of anesthesia with a digital thermometer and distal extremities temperature with the use of a thermal imaging camera attached to a smartphone. There was no significant difference between the rectal temperature before and after anesthesia within the 3 groups. Patients in group BK had a significant change in skin temperature at the end of anesthesia in all limbs (from 310C to 29.8 0C, p=0.008 and from 31 0C to 29.70C, p=0.009), respectively). Temperature variations were presented before and at the end of anesthesia, for all the groups especially at skin level. This study revealed that mobile thermal imaging represents a non-invasive technique that is helpful in assessing real time temperature changes in patients undergoing general anesthesia.
全身麻醉产生不同程度的中枢神经抑制和外周循环的改变,从而影响患者的体温调节机制。此外,缺乏适当的、专门设计的磁共振成像(MRI)环境监测设备对麻醉师来说是一个挑战。为了评估麻醉前后狗直肠和远端温度的变化,我们研究了不同麻醉方案下狗全身麻醉的温度变化。这项研究是在布加勒斯特兽医学院进行的,研究对象是21只狗,根据使用的麻醉方案分为三组。第一组(B组)给予布托啡诺(0.2 mg/kg,静脉滴注),第二组(BK组)给予布托啡诺(0.2 mg/kg)和低剂量氯胺酮(2 mg/kg)静脉滴注,第三组(BM组)给予布托啡诺(0.2 mg/kg)和咪达唑仑(0.2 mg/kg)静脉滴注。所有患者均予异丙酚(3.24±0.68)静脉诱导,气管插管,异氟醚和氧气维持。我们用数字体温计测量了麻醉前后的直肠温度,用智能手机上的热成像相机测量了远端肢体温度。三组患者麻醉前后直肠温度差异无统计学意义。BK组患者麻醉结束时四肢皮肤温度变化显著(分别从310℃至29.8℃,p=0.008,从310℃至29.70℃,p=0.009)。麻醉前和麻醉结束时的温度变化,在所有组,特别是在皮肤水平。这项研究表明,移动热成像代表了一种非侵入性技术,有助于评估全身麻醉患者的实时温度变化。
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引用次数: 0
Histological Aspects of Brunner’s Glands in Chinchillas (Chinchilla lanigera) 龙猫(Chinchilla lanigera)布鲁纳腺的组织学特征
V. Rus, F. Ruxanda, A. Damian, Florin Ghiurco, C. Martonoș, G. Dogaru, V. Miclăuș, A. Gal
Since their discovery, Brunner’s glands have been an attractive subject of research. The study aimed to investigate the structure, the topography, and the degree of development of the Brunner’s glands in chinchillas through light microscopy. Transversal fragments from the duodenum of 5 chinchilla males were histologically processed by classical paraffin technique. Brunner glands are much more developed and proportionally, they occupy at least three times more space than the Lieberkuhn glands. In many places, Brunner glands are clustered, suggesting that more than one such gland spills its secretion product through a common Lieberkuhn gland in the space between villi. We can affirm that Brunner glands, after their disposition, appearance and absence of distinct muscularis mucosae, are disposed into the thickness of the mucosa and submucosa in chinchilla (together with the Lieberkuhn glands) and not strictly in the submucosa as in other mammals.
自从布鲁纳氏腺被发现以来,它一直是一个有吸引力的研究课题。本研究旨在通过光镜观察龙猫布伦纳腺的结构、形貌和发育程度。采用经典石蜡技术对5只雄性栗鼠十二指肠的横向碎片进行了组织学处理。布鲁纳腺更发达,按比例,它们占据的空间至少是利伯库恩腺的三倍。在许多地方,布鲁纳腺聚集在一起,这表明不止一个这样的腺通过绒毛之间的一个共同的利伯库恩腺溢出其分泌产物。我们可以肯定的是,Brunner腺,在它们的分布,出现和没有明显的粘膜肌层之后,在栗鼠中被分配到粘膜和粘膜下层的厚度中(连同Lieberkuhn腺),而不是像其他哺乳动物那样严格地分配到粘膜下层。
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引用次数: 0
The Conservative Approach to Dental Crown Fractures in Dogs and Cats 犬、猫牙冠骨折的保守治疗
Raluca Ioana Nedelea, A. Toma, Andreea Mărincuș
Dental crown fractures in small animal pathology are common dental issues in every day practice. The most frequently performed procedure is extraction with all the negative consequences.The conservative approach to dental crown fractures involves endodontic treatment and coronal reconstruction in order to keep the fractured teeth on the dental arch.They were taken into study 74 dental crown fractures involving the pulp chamber, leaving the root undamaged.Clinical and radiological one-year check-up revealed us that the conservative approach to dental crown fractures in dogs and cats, when the selection criteria correspond to the indications of the specialized literature and when it is performed by latest protocols, is a reliable alternative to veterinary dental extractions.
小动物牙冠骨折是日常牙科实践中常见的问题。最常执行的程序是拔牙,所有的负面后果。牙冠骨折的保守方法包括牙髓治疗和冠状重建,以保持骨折的牙齿在牙弓上。他们研究了74个涉及牙髓腔的牙冠骨折,而牙根未受损。一年的临床和放射学检查表明,当选择标准符合专业文献的指征并按照最新的方案执行时,保守入路治疗犬和猫的牙冠骨折是一种可靠的替代兽医拔牙的方法。
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引用次数: 0
Mini-Invasive Extraarticular Stabilization of the Cranial Cruciate Deficient Stifle in Dogs 微创关节外稳定治疗犬颅十字交叉缺失症
C. Igna, D. Bumb, L. Schuszler, R. Dascalu, Vicentiu Predescu
The purpose of this work was to describe three mini-invasive techniques for extraarticular stabilization of the deficient stifle based of bone anchores inserted in quasi-isometric sites.Suture stabilization of the stifle consisted of placing a monofilament nylon leader line or polyethylene suture between femoral site adjacent to the distal poles of the fabella, and tibial site immediately cranial to the long digital extensor tendon, using two anchor types. Each stifle was tested (cranial drawer test and tibial compression test) before and after arthroscopic transection of the cranial cruciate ligament and after performing the assigned procedure. Surgical approach, order of procedural steps, drill hole entrance and exit points, and techniques for anchors placement, tensioning, and securing were described.The results of the cranial drawer test and tibial compression test do not show statistically significant differences between the three applied techniques. All three techniques provide passive stability to the deficient stifle.
这项工作的目的是描述三种微创技术,用于关节外稳定缺失的膝关节,基于在准等距位置插入骨支架。膝关节的缝合稳定包括使用两种锚钉将单丝尼龙引线或聚乙烯缝线放置在靠近腓骨远端两极的股骨部位和紧靠指长伸肌腱的胫骨部位之间。在关节镜下颅十字韧带横断术前后和执行指定手术后,对每个膝关节进行测试(颅抽屉试验和胫骨压缩试验)。本文描述了手术入路、程序步骤顺序、钻孔入口和出口点以及锚钉放置、张紧和固定的技术。颅抽屉试验和胫骨压缩试验的结果在三种应用技术之间无统计学差异。这三种技术都为缺乏的膝关节提供了被动稳定性。
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引用次数: 0
Airborne Bacteria and Fungi Concentration in Relation with Bedding Type in Broiler Houses 肉鸡舍内空气细菌和真菌浓度与垫层类型的关系
S. Popescu, C. Borda, Cristina El Mahdy, E. Lazar, A. Petrean, M. Spînu
The aim of this work was the investigation of the effect of bedding type on the airborne concentration of bacteria and fungi in broiler houses. The study was performed in two commercial farms (A and B) with different bedding (chopped straw in farm A, sunflower seed hulls in farm B). The determination of the bacteria and fungi’s numbers was performed in the first and the sixth weeks of the production cycle. Both the concentration of the bacteria and fungi showed higher values in the farm A compared with the farm B, especially in the latter period of the production cycle. In both farms, significant differences (P < 0.05) were recorded between the two periods of the determinations. The results of this study suggest that using sunflower seed hulls as bedding leads to lower levels of microbial contamination of the air in broiler houses, comparing with the use of chopped straw.
研究了不同床层类型对肉鸡鸡舍空气中细菌和真菌浓度的影响。该研究在两个商业农场(A和B)进行,采用不同的垫料(A农场切碎的稻草,B农场的葵花籽壳)。在生产周期的第1周和第6周进行细菌和真菌数量的测定。A场的细菌和真菌浓度均高于B场,特别是在生产周期的后期。在两个养殖场,两个时间段的测定结果有显著差异(P < 0.05)。本研究结果表明,与使用切碎的稻草相比,使用葵花籽壳作为垫料可以降低肉鸡舍空气中的微生物污染水平。
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引用次数: 0
A Study of Ethno-Veterinary Medicinal Plants and In Vitro Antimicrobial Activities Against Bovine Mastitis Isolated Bacterial Pathogens in Algeria 阿尔及利亚民族兽药植物及其对牛乳腺炎分离病原菌体外抗菌活性的研究
R. Saidi, N. Mimoune, R. Baazizi, M. Benaissa, D. Khelef, R. Kaidi
Bovine mastitis is the most serious dairy problem in terms of economic losses to the dairy industry. In Algeria, dominates as one of the most prevalent diseases in dairy cattle among the dairy farms. Mastitis treatment with antibiotics leads to the development of antibiotic resistant strains and consumer health problem. Multidrug-resistant bacteria have become a major health issue. With new generations of virulence and resistant bacteria, we need to improve our understanding and produce novel techniques to control these pathogenic strains. In our study, the activity of several extracts from seven medicinal plants, namely Mentha pulegium, Lavandula dentate, Origanium sp, Marrubium vulgare, Salvia bicolor, Blackstonia perfoliata, and Phlomis crinita, traditionally used in Algeria was investigated against 26 methicillin resistant staphylococci and multi-resistant Escherichia coli. (E.coli) isolated from animals with mastitis manifestation by the disc diffusion method.Results revealed the potential of extracts of Salvia bicolor, Marrubium vulgare and Phlomis crinita as antibacterial agents against strains isolated from bovine mastitis and support the possible use of these phytotherapic agents in the clinical management of the disease. Further studies into their toxicity and phytochemistry are advocated.
牛乳腺炎是乳业经济损失最严重的乳业问题。在阿尔及利亚,它是奶牛场中奶牛最流行的疾病之一。用抗生素治疗乳腺炎导致抗生素耐药菌株的发展和消费者的健康问题。耐多药细菌已成为一个主要的健康问题。随着新一代的毒力和耐药细菌的出现,我们需要提高我们的认识,并开发新的技术来控制这些致病菌株。本研究以阿尔及利亚常用的7种药用植物Mentha pulegium、Lavandula dentate、Origanium sp、Marrubium vulgare、Salvia bicolor、Blackstonia perfoliata和Phlomis crinita为原料,研究了其提取物对26种耐甲氧西林葡萄球菌和多重耐药大肠杆菌的活性。用圆盘扩散法从乳腺炎动物中分离到大肠杆菌。结果表明,双色鼠尾草、寻常凡香和灰蕨提取物对牛乳腺炎分离株具有潜在的抗菌作用,支持了这些植物治疗药物在临床治疗牛乳腺炎中的应用。建议进一步研究其毒性和植物化学。
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引用次数: 5
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine
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