Pub Date : 2015-10-08DOI: 10.15835/BUASVMCN-ASB:11608
O. Iordache, C. Cornea, C. Diguță, S. Rodino, I. Ionescu, A. Matei, I. Dumitrescu
Eight strains isolated from textile post-finishing wastewaters were subjected to molecular identification by ITS-RFLP (Internal Transcriber Spacer-Restriction Fragment Length Polymorphism) method, using ITS1 (5’-TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) DNA amplification primers for ITS region of the nuclear small-subunit rRNA gene. Restriction fragments analysis was conducted post PCR amplification, and cleaved with HaeIII, HinfI, HhaI, MseI and SduI restriction enzymes. Differences in the lengths of the obtained amplicons were detected after gel electrophoresis, and restriction analyses of the PCR fragments led to identification of six out of eight isolates as belonging to fungi T. parceramosum/ T. reesei/ T. longi, Polyporus squamosus, Fusarium oxysporum and Trichoderma atroviride groups. The PCR ITS-RFLP molecular identification technique can be used successfully for routine screening of fungal isolates from various environments.
{"title":"Molecular Identification of Fungal Isolates from Textile Wastewater by ITS-RFLP Method","authors":"O. Iordache, C. Cornea, C. Diguță, S. Rodino, I. Ionescu, A. Matei, I. Dumitrescu","doi":"10.15835/BUASVMCN-ASB:11608","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11608","url":null,"abstract":"Eight strains isolated from textile post-finishing wastewaters were subjected to molecular identification by ITS-RFLP (Internal Transcriber Spacer-Restriction Fragment Length Polymorphism) method, using ITS1 (5’-TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) DNA amplification primers for ITS region of the nuclear small-subunit rRNA gene. Restriction fragments analysis was conducted post PCR amplification, and cleaved with HaeIII, HinfI, HhaI, MseI and SduI restriction enzymes. Differences in the lengths of the obtained amplicons were detected after gel electrophoresis, and restriction analyses of the PCR fragments led to identification of six out of eight isolates as belonging to fungi T. parceramosum/ T. reesei/ T. longi, Polyporus squamosus, Fusarium oxysporum and Trichoderma atroviride groups. The PCR ITS-RFLP molecular identification technique can be used successfully for routine screening of fungal isolates from various environments.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67358343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-08DOI: 10.15835/BUASVMCN-ASB:11534
Andrada Ihuț, V. Mireșan, C. Răducu, R. Constantinescu, Luiza Andronie, C. Lațiu
Prior knowledge about correlation coefficients between major somatic characteristics can be used to guidebreeding programmes in aquaculture. The purpose of our research was to characterize the Rainbow Trout(Oncorhynchus mykiss) breeders from a phenotypical standpoint and to test the existence of correlations that canbe used in future research studies. Somatic and gravimetric measurements have been performed on 3-year oldfemales and males, obtained and raised in a regional fishery. Significant difference (646.11 g; p 0.05). The outcome values reflect a higher interdependence between some studied characteristics in males, ascompared to females, as indicated by Pearson’s correlation coefficients and open the direction in which the realselection can be done.
{"title":"Conformation features of three-year old rainbow trout (Oncorhynchus mykiss) breeding stock from a trout farm.","authors":"Andrada Ihuț, V. Mireșan, C. Răducu, R. Constantinescu, Luiza Andronie, C. Lațiu","doi":"10.15835/BUASVMCN-ASB:11534","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11534","url":null,"abstract":"Prior knowledge about correlation coefficients between major somatic characteristics can be used to guidebreeding programmes in aquaculture. The purpose of our research was to characterize the Rainbow Trout(Oncorhynchus mykiss) breeders from a phenotypical standpoint and to test the existence of correlations that canbe used in future research studies. Somatic and gravimetric measurements have been performed on 3-year oldfemales and males, obtained and raised in a regional fishery. Significant difference (646.11 g; p 0.05). The outcome values reflect a higher interdependence between some studied characteristics in males, ascompared to females, as indicated by Pearson’s correlation coefficients and open the direction in which the realselection can be done.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67357971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/BUASVMCN-ASB:11568
Mirela Cadar
Hair fibre study is very actual due to its implications especially in forensic medical expertise. The usual and SEM microscopic morphology of primary hair fibres collected from mature male dogs of five well-known breeds: Husky, Bichon Maltese, Golden retriever, Labrador and German Shepherd were studied. Descriptive observations and quantitative data obtained by micrometry do not lead to significant differences to permit identification of one breed by simple hair examination. Nonetheless, comparing the quantitative parameters of hair fibre obtained in our study with those known in specialty literature on human hair, we can conclude that there are differences among medullary index values and the index could be an identification criterion.
{"title":"SEM study of hair cuticle in some Canidae breeds.","authors":"Mirela Cadar","doi":"10.15835/BUASVMCN-ASB:11568","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11568","url":null,"abstract":"Hair fibre study is very actual due to its implications especially in forensic medical expertise. The usual and SEM microscopic morphology of primary hair fibres collected from mature male dogs of five well-known breeds: Husky, Bichon Maltese, Golden retriever, Labrador and German Shepherd were studied. Descriptive observations and quantitative data obtained by micrometry do not lead to significant differences to permit identification of one breed by simple hair examination. Nonetheless, comparing the quantitative parameters of hair fibre obtained in our study with those known in specialty literature on human hair, we can conclude that there are differences among medullary index values and the index could be an identification criterion.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67358041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/BUASVMCN-ASB:11356
A. Șara, M. Bențea
Abstract Research carried out by the Chemical SRL Company in partnership with the University of Milan on broiler chicken, regarding the replacement of soybean meal with guar meal (Guar 60PF and Guar 70 PFR) highlighted a higher body mass for the groups that received Guar 70 PFR and Guar 60 PF, the control group showing smaller body mass values. Regarding the average daily gain, the higher values were recorded in the experimental groups, the highest value being recorded in Guar 70 PFR group, followed by the Guar 60 PF group, and the control group. The food conversion ratio was lower for the Guar 70 PFR and Guar 60 PF groups. Other authors reported that guar meal administered in low doses (2.5% and 5%) in feeds had no negative effects on the growth performances of broiler chickens, whereas higher doses (7.5 and 10%) negatively influenced both growth performances and broiler chickens’ health. After partially replacing the soybean meal with guar meal in broiler chicken feeds, no negative effects on body mass evolution, weight gain, feed conversion and carcass quality in broiler chicken were reported.   Â
化学SRL公司与米兰大学合作对肉鸡进行了一项研究，关于用瓜尔粉(瓜尔60PF和瓜尔70 PFR)代替豆粕的研究表明，使用瓜尔70 PFR和瓜尔60PF的组的体重更高，对照组的体重值较小。平均日增重以试验组最高，瓜尔70 PFR组最高，瓜尔60 PFR组次之，对照组最高。瓜尔70 PFR组和瓜尔60 PF组的食物转化率较低。其他作者报道，低剂量(2.5%和5%)瓜尔粉饲料对肉鸡的生长性能没有负面影响，而高剂量(7.5%和10%)对肉鸡的生长性能和健康都有负面影响。用瓜尔豆粕部分替代肉鸡饲料中的豆粕，对肉鸡体质量演变、增重、饲料转化率和胴体品质均无负面影响。Â Â Â
{"title":"The Effects of Guar Meal on Production Performances in Broiler Chicken","authors":"A. Șara, M. Bențea","doi":"10.15835/BUASVMCN-ASB:11356","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11356","url":null,"abstract":"Abstract Research carried out by the Chemical SRL Company in partnership with the University of Milan on broiler chicken, regarding the replacement of soybean meal with guar meal (Guar 60PF and Guar 70 PFR) highlighted a higher body mass for the groups that received Guar 70 PFR and Guar 60 PF, the control group showing smaller body mass values. Regarding the average daily gain, the higher values were recorded in the experimental groups, the highest value being recorded in Guar 70 PFR group, followed by the Guar 60 PF group, and the control group. The food conversion ratio was lower for the Guar 70 PFR and Guar 60 PF groups. Other authors reported that guar meal administered in low doses (2.5% and 5%) in feeds had no negative effects on the growth performances of broiler chickens, whereas higher doses (7.5 and 10%) negatively influenced both growth performances and broiler chickens’ health. After partially replacing the soybean meal with guar meal in broiler chicken feeds, no negative effects on body mass evolution, weight gain, feed conversion and carcass quality in broiler chicken were reported.   Â","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/BUASVMCN-ASB:11356","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67356741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/BUASVMCN-ASB:11422
F. Popescu, C. Lațiu, D. Cocan, V. Mireșan
Abstract The problem of invasive species of fish is a major issue, both internationally and nationally. The presence of invasive species is often associated with acclimatization of new species of fish, activities correlated with the absence of effective control and quarantine programs. In other words, when new desired species are imported, other accompanying species are also brought in often in the form of embryonated eggs or juveniles.The brown bullhead, which we have studied, is also an invasive species of fish in Stejeris Lake, Cluj County. This species is of interest, both scientifically and economically. From the scientific point of view, brown bullhead has a high capacity of resistance to environmental changes, unfavourably conditions, which ensures a perpetuation of the species in almost every aquatic environment. Even though it is recognized that the economic impact of this invasive species is significant, not much information is available at present, and neither is there much data about the topography of its internal organs, which is being studied in this paper.
{"title":"Topography of the Internal Organs of the Brown Bullhead (Ameiurus nebulosus) Lesueur, 1819 (Actinopterygii: Ictaluridae)","authors":"F. Popescu, C. Lațiu, D. Cocan, V. Mireșan","doi":"10.15835/BUASVMCN-ASB:11422","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11422","url":null,"abstract":"Abstract The problem of invasive species of fish is a major issue, both internationally and nationally. The presence of invasive species is often associated with acclimatization of new species of fish, activities correlated with the absence of effective control and quarantine programs. In other words, when new desired species are imported, other accompanying species are also brought in often in the form of embryonated eggs or juveniles.The brown bullhead, which we have studied, is also an invasive species of fish in Stejeris Lake, Cluj County. This species is of interest, both scientifically and economically. From the scientific point of view, brown bullhead has a high capacity of resistance to environmental changes, unfavourably conditions, which ensures a perpetuation of the species in almost every aquatic environment. Even though it is recognized that the economic impact of this invasive species is significant, not much information is available at present, and neither is there much data about the topography of its internal organs, which is being studied in this paper.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67357524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/BUASVMCN-ASB:11332
C. Pascal, V. Gašpar
Forming sheepdog breeds has had primarily economic and utility objectives and is the direct result of the influence of natural factors, or of reproductive isolation, a process which resulted in natural breeds characterized by genetic, phenotypical and behavioural uniformity. The current economic importance of breeding Bucovina Shepherd dogs is very well highlighted by the fact that in the area of origin there appeared a number of kennels, in which selection programs and routed mating are applied in order to increase the quality of genetic material and to enhance the skills of this dog. From this point of view, we analyse the existence of genetic correlations between certain morphometric characteristics in Bucovina Shepherd dogs, and carry out a comprehensive study on some elements that make the difference among the four breeds of shepherd dogs raised in Romania. These endeavours are part of current concerns addressed in animal husbandry. Biological material subject to investigation was represented by the canine population belonging to the Bucovina Shepherd Dog breed, raised in the North-Eastern part of Romania. As a result of statistical data processing and interpretation, it was established that for most attributes, in the couples analysed, the correlation coefficient exceeds 0.400 which means, in general, that the analysed characteristics are well correlated. However, there are also weakly correlated characteristics (r< 0.2) as is the case of dorsum height and hip height. Instead, the correlation between the height of the base of the tail and back height was positive and intense (r = 0.98), which means that the selection applied for one of these two characteristics leads to the improvement of the other characteristic. Also, as a general conclusion of the study, the results could be applied in the improvement of this sheepdog population because the correlation coefficient values recorded between the main characteristics analysed have been statistically distinctly significant (p < 0.01%).
{"title":"Determining the Correlation Between Specific Characters of Bucovina Shepherd Dogs","authors":"C. Pascal, V. Gašpar","doi":"10.15835/BUASVMCN-ASB:11332","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11332","url":null,"abstract":"Forming sheepdog breeds has had primarily economic and utility objectives and is the direct result of the influence of natural factors, or of reproductive isolation, a process which resulted in natural breeds characterized by genetic, phenotypical and behavioural uniformity. The current economic importance of breeding Bucovina Shepherd dogs is very well highlighted by the fact that in the area of origin there appeared a number of kennels, in which selection programs and routed mating are applied in order to increase the quality of genetic material and to enhance the skills of this dog. From this point of view, we analyse the existence of genetic correlations between certain morphometric characteristics in Bucovina Shepherd dogs, and carry out a comprehensive study on some elements that make the difference among the four breeds of shepherd dogs raised in Romania. These endeavours are part of current concerns addressed in animal husbandry. Biological material subject to investigation was represented by the canine population belonging to the Bucovina Shepherd Dog breed, raised in the North-Eastern part of Romania. As a result of statistical data processing and interpretation, it was established that for most attributes, in the couples analysed, the correlation coefficient exceeds 0.400 which means, in general, that the analysed characteristics are well correlated. However, there are also weakly correlated characteristics (r< 0.2) as is the case of dorsum height and hip height. Instead, the correlation between the height of the base of the tail and back height was positive and intense (r = 0.98), which means that the selection applied for one of these two characteristics leads to the improvement of the other characteristic. Also, as a general conclusion of the study, the results could be applied in the improvement of this sheepdog population because the correlation coefficient values recorded between the main characteristics analysed have been statistically distinctly significant (p < 0.01%).","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67356395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/BUASVMCN-ASB:11331
I. Nechifor, C. Pascal, M. Florea
The Karakul of Botosani sheep breed raises genuine interest of breeders in North-Eastern Romania. Therefore, pelt quality assessment and studying of the mode of colour transmission are on-going activities for purposes of improvement. Lambs are obtained from the breeding of genitors belonging to the black colour variety. Breeding was based on matching pairs, involving 193 females, taking into account their line of origin and the pursued goal of the improvement. The appraisal method of transmission of colour and characteristics on which the quality of pelts depend was made in the first 24 hours after birth, based on the technical specifications and instructions in the certificate of origin, and productive values. Classification of lambs in relation to productive performances was done on the basis of the total final score. The study was conducted over a period of five generations of lambs obtained in calving seasons between 2010 and 2014. From the crossbreeding analysis of the genitors of black colour variety over several generations, it was established that 98% of the offspring had uniform black colour, but with different shades, and only 2% had other colours. This data allow us to conclude that the black colour specific of the Karakul of Botosani sheep is a dominant type.
{"title":"The color transmission in the case of cross breed sheep belonging to the black Karakul variety.","authors":"I. Nechifor, C. Pascal, M. Florea","doi":"10.15835/BUASVMCN-ASB:11331","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11331","url":null,"abstract":"The Karakul of Botosani sheep breed raises genuine interest of breeders in North-Eastern Romania. Therefore, pelt quality assessment and studying of the mode of colour transmission are on-going activities for purposes of improvement. Lambs are obtained from the breeding of genitors belonging to the black colour variety. Breeding was based on matching pairs, involving 193 females, taking into account their line of origin and the pursued goal of the improvement. The appraisal method of transmission of colour and characteristics on which the quality of pelts depend was made in the first 24 hours after birth, based on the technical specifications and instructions in the certificate of origin, and productive values. Classification of lambs in relation to productive performances was done on the basis of the total final score. The study was conducted over a period of five generations of lambs obtained in calving seasons between 2010 and 2014. From the crossbreeding analysis of the genitors of black colour variety over several generations, it was established that 98% of the offspring had uniform black colour, but with different shades, and only 2% had other colours. This data allow us to conclude that the black colour specific of the Karakul of Botosani sheep is a dominant type.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/BUASVMCN-ASB:11331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67356611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/BUASVMCN-ASB:11421
Flavius Banyai, A. Vlaic
Abstract In Romanian horse breeds there is little information about Polysaccharide Storage Myopathy disease and therefore the objective of this study was to do a first evaluation of two indigenous horse breeds for presence of polymorphism in the GYS1 gene, associated with the disease. In this purpose, a number of 14 biological samples were collected from two breeds : Semigreu Romanesc and Lipitan. The genomic DNA was isolated from hair samples with Quick-gDNA Mini Prep kit, according to the manufacturer’s protocol. A 229 bp fragment, targeting the polymorphic region of GYS1 gene, was amplified with a set of specific primers and the PCR products were digested with HpyCH4 V restriction enzyme. The resulting fragments were separated in 3% agarose gel. Two fragments, of 151 and 78 bp, which correspond to the GG genotype, were identified, while four fragments (151, 97, 78 and 54 bp) were obtained in the case of heterozygous AG genotypes. All analysed Lipitan horses were homozygous for the G allele, while all Semigreul Romanesc horses were heterozygous, carrying the A allele which are associated with PSSM. To establish more accurate results in Romanian horse breeds, further studies, on a larger number of horses, are needed.
在罗马尼亚马品种中，关于多糖储存性肌病的信息很少，因此本研究的目的是首次评估两个地方马品种中与该疾病相关的GYS1基因多态性的存在。为此，从罗马半猪和利皮坦猪两个品种采集了14份生物样品。根据制造商的方案，使用Quick-gDNA Mini Prep试剂盒从头发样本中分离基因组DNA。利用一组特异性引物扩增GYS1基因多态性区229 bp片段，用HpyCH4 V限制性内切酶酶切。所得片段在3%琼脂糖凝胶中分离。GG基因型共鉴定出151和78 bp的片段，AG基因型共鉴定出151、97、78和54 bp的片段。所有利比坦马的G等位基因均为纯合子，而所有半格鲁罗曼马的G等位基因均为杂合子，携带与PSSM相关的A等位基因。为了在罗马尼亚马品种中建立更准确的结果，需要对更多的马进行进一步的研究。
{"title":"Polysaccharide Storage Myopathy (PSSM) - a genetically determined disease in horses.","authors":"Flavius Banyai, A. Vlaic","doi":"10.15835/BUASVMCN-ASB:11421","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11421","url":null,"abstract":"Abstract In Romanian horse breeds there is little information about Polysaccharide Storage Myopathy disease and therefore the objective of this study was to do a first evaluation of two indigenous horse breeds for presence of polymorphism in the GYS1 gene, associated with the disease. In this purpose, a number of 14 biological samples were collected from two breeds : Semigreu Romanesc and Lipitan. The genomic DNA was isolated from hair samples with Quick-gDNA Mini Prep kit, according to the manufacturer’s protocol. A 229 bp fragment, targeting the polymorphic region of GYS1 gene, was amplified with a set of specific primers and the PCR products were digested with HpyCH4 V restriction enzyme. The resulting fragments were separated in 3% agarose gel. Two fragments, of 151 and 78 bp, which correspond to the GG genotype, were identified, while four fragments (151, 97, 78 and 54 bp) were obtained in the case of heterozygous AG genotypes. All analysed Lipitan horses were homozygous for the G allele, while all Semigreul Romanesc horses were heterozygous, carrying the A allele which are associated with PSSM. To establish more accurate results in Romanian horse breeds, further studies, on a larger number of horses, are needed.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15835/BUASVMCN-ASB:11421","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67356890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/BUASVMCN-ASB:11455
C. Daniel, Iulius Rotar, Luisa Andronie, O. Negrea, Călin Laţiu, R. Constantinescu, Camelia Răducu, M. Vioara
Abstract In this study the structure of the Danube crayfish (Astacus leptodactylus) population from A¢aga Mare Lake, Cluj County was analyzed. According to the study results, three age categories (2+, 3+ and 4+) were identified for the Danube crayfish specimens. Numerically, females are more numerous than males, the sex ratio between males and females being as follows: SR2+ = 0.33; SR3+ = 0.33; SR4+ = 0.86. In terms of body size, males are better developed, except for their abdominal segments, where females are better developed due to the female reproductive function.
{"title":"Danube Crayfish Astacus leptodactylus (Eschscholtz, 1823) from Å¢aga Mare Lake – Size and Population Structure","authors":"C. Daniel, Iulius Rotar, Luisa Andronie, O. Negrea, Călin LaÅ£iu, R. Constantinescu, Camelia Răducu, M. Vioara","doi":"10.15835/BUASVMCN-ASB:11455","DOIUrl":"https://doi.org/10.15835/BUASVMCN-ASB:11455","url":null,"abstract":"Abstract In this study the structure of the Danube crayfish (Astacus leptodactylus) population from A¢aga Mare Lake, Cluj County was analyzed. According to the study results, three age categories (2+, 3+ and 4+) were identified for the Danube crayfish specimens. Numerically, females are more numerous than males, the sex ratio between males and females being as follows: SR2+ = 0.33; SR3+ = 0.33; SR4+ = 0.86. In terms of body size, males are better developed, except for their abdominal segments, where females are better developed due to the female reproductive function.","PeriodicalId":9587,"journal":{"name":"Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67357657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-10-07DOI: 10.15835/buasvmcn-asb:11357
M. Bențea, A. Șara
Abstract Guar ( Cyamopsis tetragonoloba ) is an annual pulse from the dry tropical regions; said to originate from the wild African species C. senegalesis . Although the leaves and pods can also be used, the seeds are the most used part of the plant. It has high protein content, with high digestibility and palatability without any toxicity sign, and can be used in farm animal nutrition as protein products. Guar meal is submitted to heat treatment to inhibit the anti-nutritive factors and significantly increase the product’s digestibility and palatability. The Milan based company, Chemical SRL, produces a number of guar meal types such as GUAR 50PF, Guar 60PF and Guar 70PFR. This product can be used for all farm animals, especially cows, swine, poultry and fish, as it is not genetically modified (GMO-Free) and no other chemical substances are used during its fabrication process.
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