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Effect of Pesticides on Enzymatic Activity in Soil 农药对土壤酶活性的影响
M. Micuți, L. Bădulescu, F. Israel-Roming
The focus of this article is to provide informations about soil enzymatic activity as a biological indicator for impacts of pesticides on soils. In this experiment, an ecological soil was treated with two types of fungicide (Ridomil Gold and Bravo 500) and two of insecticides (Mospilan 20SG and Vertimec 1.8% EC). The pesticides were assessed for their effect on different enzymatic activities. They were administrated over a 28 days period and the samples of soil were taken once every 7 days and analyzed in the laboratory. For each sample was determined the enzymatic activity, pH, humidity. The enzymatic activity was assessed using colorimetrical methods. Enzymes chosen for this study were cellulase, amylase, xylanase, urease, alkaline and acid phosphatase. Results shown that the enzymatic activity can increase or decrease when the soil was treated with the fungicides and insecticides chosen for this experiment.
本文的重点是提供土壤酶活性作为农药对土壤影响的生物学指标的信息。本试验采用两种杀菌剂(Ridomil Gold和Bravo 500)和两种杀虫剂(Mospilan 20SG和Vertimec 1.8% EC)处理生态土壤。评价了农药对不同酶活性的影响。施用28天,每7天采集一次土壤样本,在实验室进行分析。测定每个样品的酶活性、pH值、湿度。用比色法测定酶活性。选用的酶有纤维素酶、淀粉酶、木聚糖酶、脲酶、碱性和酸性磷酸酶。结果表明,施用本试验选用的杀菌剂和杀虫剂后,土壤酶活性可提高也可降低。
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引用次数: 6
Germination, in vitro Propagation and Acclimatization in Lavandula Angustifolia 薰衣草的萌发、离体繁殖和驯化
I. Miclea, Raluca Chifor
This research focused on finding the best method for seed in vitro germination in Lavandula angustifolia and optimizing the medium for plant propagation. Seeds were sterilized and subjected to various treatments to break dormancy, then placed on half-strength MS (1/2MS) or distilled H2O + phytagel. Germination percentages were assessed and plantlets propagated on MS without growth regulators or with zeatin (0.5, 1, 2 mg/l), 1 mg/l BA + 0.5 mg/l IBA, 2 mg/l BA + 1 mg/l IBA or 3 mg/l BA + 1.5 mg/l IBA. After 8 weeks growth parameters were recorded and plants were acclimatized. Immersion in 20 mg/100 ml GA3 solution for 24 hours at 4°C was the most effective in breaking dormancy. Stratification at 4°C for 8 weeks and soaking in a solution of 0.5% H2O2 at 23°C for 24 hours also proved beneficial but to a smaller degree. Half-strength MS was the best germination medium. Shoot development was the highest in MS supplemented with zeatin (2 or 1 mg/l) while roots formed only in the control. Callus induction percentage was best in the presence of 3 mg/l BA + 1.5 mg/l IBA but decreasing concentrations increased callus weight. Plant acclimatization was more successful in moss:sand - 1:2 than in vermiculite:perlite:sand - 2:2:1.
本研究旨在寻找薰衣草种子离体萌发的最佳方法,并对植物繁殖的培养基进行优化。对种子进行灭菌,并进行各种处理以打破休眠,然后放置在半强度MS (1/2MS)或蒸馏H2O + phytagel上。在不添加生长调节剂或添加玉米素(0.5、1、2 mg/l)、1 mg/l BA + 0.5 mg/l IBA、2 mg/l BA + 1 mg/l IBA或3 mg/l BA + 1.5 mg/l IBA的培养基上进行繁殖。8周后记录生长参数并进行驯化。在20 mg/100 ml GA3溶液中浸泡24小时,在4°C下打破休眠最有效。在4°C下分层8周,在23°C的0.5% H2O2溶液中浸泡24小时也证明是有益的,但程度较小。半强度MS为最佳萌发培养基。在添加玉米素(2或1 mg/l)的MS中,茎部发育最高,而根只在对照中形成。BA浓度为3 mg/l + IBA浓度为1.5 mg/l时愈伤组织诱导率最高,但浓度越低愈伤组织质量越大。苔藓:沙子:1:2比蛭石:珍珠岩:沙子:2:2:1的植物驯化更成功。
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引用次数: 5
Effect of Dietary Extruded Linseed and Walnut Meal Mixture (8:1) on Performance and Plasma Protein Profile in Weaned Piglets 亚麻仁和核桃仁混合粉(8:1)对断奶仔猪生产性能和血浆蛋白水平的影响
A. Gheorghe, M. Hăbeanu, N. Lefter, D. Grigore
The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on performance and plasma protein profile in weaned piglets was evaluated for 21 d. Topigs piglets (n=40; BW=8.02±0.82 kg), age 30±3 days, were allotted into 2 groups and fed 2 diets: control [C, based on corn-triticale-soybean meal (SBM)] and experimental (ELS:WM, where the ELS:WM mixture (8:1) partially replace SBM). Blood samples were collected at 7d and 21d after weaning. The plasma protein profile (total protein, total bilirubin, albumin, creatinine, uric acid, urea nitrogen-BUN) were determined by a chemistry analyser. Dietary ELS:WM mixture improve the BW (P=0.047) and ADG (P=0.036) of piglets at 21d after weaning vs C, whereas ADFI and F:G ratio were increase (P>0.05). The plasma protein profile of piglets fed dietary mixture was not affected at 7d and 21d after weaning, except plasma BUN concentration that was decrease (P=0.027) at 21d after weaning. Lower BUN concentration indicated higher availability of dietary nitrogen reflected in a higher deposition of protein. We concluded that dietary ELS:WM mixture improve piglets performance and positively afect plasma protein profile, especially BUN in weaning period.
评价了日粮挤压亚麻籽(ELS):核桃粉(WM)混合物(8:1)对断奶仔猪生产性能和血浆蛋白水平的影响,分为2组,饲喂2种日粮:对照组[C,基于玉米-小黑麦-豆粕(SBM)]和实验组(ELS:WM,其中ELS:WM混合物(8:1)部分取代SBM)。断奶后第7天和第21天采集血样。通过化学分析仪测定血浆蛋白谱(总蛋白、总胆红素、白蛋白、肌酸酐、尿酸、尿素氮BUN)。与对照组相比,ELS:WM混合日粮可提高断奶后21d仔猪的BW(P=0.047)和ADG(P=0.036),而ADFI和F:G比值则有所增加(P>0.05)。较低的BUN浓度表明日粮氮的可用性较高,这反映在较高的蛋白质沉积中。我们得出的结论是,ELS:WM混合物可以改善仔猪的生产性能,并对断奶期的血浆蛋白水平,尤其是BUN产生积极影响。
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引用次数: 5
Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains 蜜蜂面包提取物对不同菌株的抑菌活性研究
A. Urcan, A. Criste, D. Dezmirean, O. Bobiș, L. Mǎrghitaş, Rodica Mărgăoan, Alexandra Hrinca
Bee bread is a product of the hive obtained from pollen collected by bees, to which they add honey, digestive enzymes and subsequently is stored in the combs. Increasing evidence suggests bee bread’s potential therapeutic benefits, including antimicrobial properties. Bee bread is characterized by a bactericidal and bacteriostatic activity. The current study was carried out to test the antimicrobial activity of bee bread extracts, in various concentrations, against the bacterial strains: Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella enterica and Pseudomonas aeruginosa. The results of this study indicate that the first two dilutions of bee bread extract, respectively 33% and 16.66%, showed higher antimicrobial activity and the other dilutions had a lower, but visible activity depending on the pathogen on which they are tested. The best antimicrobial activity was manifested on the Staphylococcus aureus strain, where all dilutions had an inhibitory effect both at 8 hours and 12 hours.
蜜蜂面包是从蜜蜂收集的花粉中获得的蜂巢产品,蜜蜂在其中添加蜂蜜和消化酶,然后储存在蜂巢中。越来越多的证据表明蜜蜂面包具有潜在的治疗益处,包括抗菌特性。蜂面包的特点是具有杀菌和抑菌活性。本研究旨在测试不同浓度的蜂面包提取物对以下菌株的抗菌活性:蜡样芽孢杆菌、金黄色葡萄球菌、大肠杆菌、肠炎沙门氏菌和铜绿假单胞菌。这项研究的结果表明,蜜蜂面包提取物的前两种稀释液,分别为33%和16.66%,显示出较高的抗菌活性,而其他稀释液的抗菌活性较低,但可见,这取决于它们所测试的病原体。最佳的抗菌活性表现在金黄色葡萄球菌菌株上,所有稀释液在8小时和12小时都具有抑制作用。
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引用次数: 13
Risks and Benefits of Food Additives - Review 食品添加剂的风险和益处——综述
C. Pașca, A. Coroian, S. Socaci
Food additives are substances of natural or synthetic origin, which are added to foods to serve a certain technological or sensory function, for example, to counter food perishability and bacterial degradation, give or restore color or impart flavor to foods.These additives generally provide some type of benefit for the food producer, processor or consumer. For example: acids that may be added to prevent the growth of microorganisms that cause spoilage may also prevent the growth of microorganisms that can cause foodborne illness. Some additives are directly added to food and ingredients, while others are added indirectly through contact with packaging materials as are, for example the preservatives BHA and BHT on the inside of breakfast cereal bags.The benefit of some food additives is enhancing health status or prevents disease; most benefits reflect economic considerations for food processors and sensory attributes and convenience for consumer.
食品添加剂是天然或合成来源的物质,添加到食品中是为了发挥一定的技术或感官功能,例如,对抗食物的易腐性和细菌降解,赋予或恢复食物颜色或赋予食物风味。这些添加剂通常为食品生产商、加工商或消费者提供某种类型的益处。例如:可以添加酸来防止导致腐败的微生物生长,也可以防止导致食源性疾病的微生物生长。一些添加剂是直接添加到食品和配料中的,而另一些则是通过与包装材料接触而间接添加的,例如早餐麦片袋内部的防腐剂BHA和BHT。一些食品添加剂的好处是提高健康状况或预防疾病;大多数好处反映了食品加工商的经济考虑、感官特性和消费者的便利性。
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引用次数: 11
Piglets’ Intestinal Microflora Fed with a Plants Mix 混合植物喂养的仔猪肠道微生物群
G. M. Cornescu, T. Panaite, T. Gavris, C. Tabuc, M. Olteanu, R. Criste
The objective of this study was to evaluate the effect of a plant mix (bilberry, black currant, quince, peppermint and fennel essential oil) inclusion into the diets of piglets (18-45 kg) on intestinal microflora equilibrium. An experiment was performed on 8 castrated hybrid TOPIGS (18.69±1.25 kg) divided in 2 groups (C and E). The piglets were kept in an experimental house in individual metabolic cages. Compared to the conventional diet (18% crude protein and 3214 kcal/kg metabolic energy) of group C, the diet of E group had included 789 mg mixture of plants/kg feed. At the final of the experiment the piglets were slaughtered and digesta samples were collected from jejunum and ileum for microbiological analysis. For jejunum, a significant (P<0.05) decrease of Staphylococci spp. (log10 CFU/g) concentration in E group was noticed correlated strongly negative with a statistically significant (P<0.05) increasing concentration of Lactobacillus spp. (log10 CFU/g) concentration. The results were similar for ileum. This dietary mixture of plants had some effects on microbial population of piglets’ jejunum and ileum to help positively the intestinal changes of microbiota.
本研究的目的是评估在仔猪(18-45公斤)日粮中加入植物混合物(越橘、黑加仑、木瓜、薄荷和茴香精油)对肠道菌群平衡的影响。对8只去势杂交TOPIGS(18.69±1.25kg)进行了实验,分为两组(C组和E组)。小猪被关在一个单独的新陈代谢笼子里的实验室里。与C组的传统日粮(18%粗蛋白和3214 kcal/kg代谢能)相比,E组的日粮包括789mg植物混合物/kg饲料。在实验的最后,屠宰仔猪,并从空肠和回肠收集消化物样本进行微生物分析。对于空肠,葡萄球菌属(log10CFU/g)的浓度显著降低(P<0.05)。E组的浓度与乳酸杆菌属(log10CFU/g)的浓度增加(P<0.05)呈强负相关。回肠的结果相似。这种植物混合饲料对仔猪空肠和回肠的微生物种群有一定的影响,有助于促进肠道微生物群的变化。
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引用次数: 0
Economic Effects of Dairy Cow’s Diet Supplementation with Anionic Salts 奶牛日粮中添加阴离子盐的经济效应
M. Bogdan, M. Bențea, A. Șara
This research proves the economic effect of Calving Care performance in dairy cow feeding, on Holstein Friesian cows. Group M1 / 2014 of 10 cows in second lactation are the control group in which Calving Care was not administered, monitoring performances throughout the lactation of 2014. Group E / 2015 is the experimental group, where the same cows receivedCalving Care 300 g / cow / day, 21 days before calving. Dietary cation-anion difference was -15.28 mEq / 100 g dry matter. Group M2 / 2016 was the second control group, without Calving Care, productive performance in the following lactation was monitored.After calving, each cow has been monitored for Fat corrected milk (FCM) production per 305 days lactation and dry matter intake, ratio between these parameters being expressed asmilk production efficiency. Milk efficiency was 1.25 in Group M1/2014, 1.3 in Group E/2015 and 1.28 in Group M2/2016 respectively.
本研究证明了产仔护理在奶牛饲养中对荷斯坦-弗里斯奶牛的经济影响。M1/2014组10头处于第二次泌乳期的奶牛是未进行产仔护理的对照组,监测2014年整个泌乳期的表现。E/2015组为实验组,相同的奶牛在产仔前21天接受300克/头牛/天的断奶护理。日粮阴阳离子差异为-15.28mEq/100g干物质。M2/2016组是第二个对照组,在没有产仔护理的情况下,监测接下来哺乳期的生产表现。产仔后,对每头牛每305天泌乳期的脂肪校正乳(FCM)产量和干物质摄入量进行监测,这些参数之间的比率表示为牛奶生产效率。M1/2014组的牛奶效率分别为1.25,E/2015组为1.3,M2/2016组为1.28。
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引用次数: 0
The Actual Situation of Sheep’s Wool Capitalization in Romania 罗马尼亚羊毛资本化的实际情况
I. Florea
Sheep farming always made a special contribution to the development of practicing people, improving the standard of living, effects also known by Romanians, even if the number of sheep and implicitly the amount of wool had fluctuations over time. After the 90s, demand for wool in the textile industry began to decline and the wool has not been widely capitalized in Romania, being abandoned by farmers, even burned. The aim of this study is to provide solutions for wool collection in an organized manner and as profitable as possible for Romanians at national level, avoiding wool waste and chaotic export. The collection centers are unevenly distributed accros the country and there are some counties where sheep wool is not collected. With the proposed solution of locating other collection centers or distributing the authorized ones, it is possible to capitalize the entire wool resource available in Romania.
尽管羊的数量和羊毛的数量会随着时间的推移而波动,但羊的养殖对人们的发展和生活水平的提高总是做出了特殊的贡献,罗马尼亚人也知道这种影响。90年代以后,纺织行业对羊毛的需求开始下降,羊毛在罗马尼亚没有被广泛资本化,被农民抛弃,甚至被焚烧。本研究的目的是在国家层面上为罗马尼亚人提供有组织的、尽可能有利可图的羊毛收集解决方案,避免羊毛浪费和混乱的出口。收集中心在全国分布不均匀,有一些县没有收集羊毛。根据建议的解决方案,找到其他收集中心或分销授权的收集中心,可以利用罗马尼亚可用的整个羊毛资源。
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引用次数: 1
Nutritional Fortification of Sunflower Meal by Bacillus Subtilis ATCC PTA-6737 Fermentation 枯草芽孢杆菌ATCC PTA-6737发酵向日葵粕的营养强化研究
S. Yasar, Caner Uysal, R. Tosun
Sunflower meal with 3 mm particle size (SFM) with 66 or 80% moisture content was inoculated with 1x107 cfu (colony forming unit/g) of Bacillus subtilis ATCC PTA-6737 and fermented for 0, 24, 48 and 72 h. The pH was increased from 5.75 to 9.1 of SFM fermented at both moisture levels with no significant changes in organic acid production. The bacterial growth was peaked at 24 h. Dry matter and crude fibre contents of SFM decreased by 10-13 and 18%, respectively. In contrary, the amount of crude protein, crude ash and soluble amino acid nitrogen increased by 36, 61% and 145%, respectively, with no regard to the effect of moisture content. Phytic acid was degraded up to 42% at both level of moisture content. In conclusion, SFM was enriched with ash and protein and lowered in fibre and phytic acid contents, and can be used as alternative feed material in animal nutrition.
用1x107cfu(菌落形成单位/g)的枯草芽孢杆菌ATCC PTA-6737接种水分含量为66%或80%的3mm颗粒大小的向日葵粉(SFM),并发酵0、24、48和72小时。在两个水分水平下发酵的SFM的pH从5.75增加到9.1,有机酸产量没有显著变化。细菌生长在24小时达到峰值。SFM的干物质和粗纤维含量分别下降了10-13%和18%。相反,在不考虑水分含量影响的情况下,粗蛋白质、粗灰分和可溶性氨基酸氮的含量分别增加了36%、61%和145%。在两种含水量水平下,Phytic酸的降解率都高达42%。总之,SFM富含灰分和蛋白质,纤维和植酸含量较低,可作为动物营养的替代饲料材料。
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引用次数: 2
Comparison of ABTS, DPPH, Phosphomolybdenum Assays for Estimating Antioxidant Activity and Phenolic Compounds in Five Different Plant Extracts ABTS、DPPH、磷钼法测定5种不同植物提取物抗氧化活性及酚类化合物的比较
A. Untea, A. Lupu, M. Sărăcilă, T. Panaite
Many plants and their constituents (carotenoids, flavonoids and other phenolic compounds), action to inhibit the free radical by the use of antioxidant activity, helping to a better efficiency of nutrients utilization. The purpose of this study was to investigate the antioxidant activity and phenolic content of five different plants, known for their antioxidant potential. Total phenols were determined by FolinCiocalteumethod, with few modifications. The antioxidant capacity was evaluated by DPPH, ABTS radical-scavenging activity and phosphomolybdenum method. The results showed that the highest antioxidant activity was obtained for walnut leaves in terms of ABTS evaluation and for bilberry leaves by DPPH and phosphomolybdenum methods. In the present study, peppermint, walnut and bilberry leaves proved to have an important antioxidant activity and also a total phenolic content.
许多植物及其成分(类胡萝卜素、类黄酮和其他酚类化合物),通过抗氧化活性抑制自由基的作用,有助于更好地利用营养物质。本研究的目的是研究五种不同植物的抗氧化活性和酚含量,这些植物被认为具有抗氧化潜力。总酚用folincocalteu法测定,修改较少。采用DPPH、ABTS自由基清除活性和磷钼法评价其抗氧化能力。结果表明,用ABTS评价核桃叶的抗氧化活性最高,用DPPH和磷钼法评价越橘叶的抗氧化活性最高。在本研究中,薄荷、核桃和越橘叶被证明具有重要的抗氧化活性和总酚含量。
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引用次数: 24
期刊
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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