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Studies regarding the biological parameters of Azov shad (Alosa tanaica, Grimm, 1901) from the Romanian Black Sea Coast. 罗马尼亚黑海沿岸亚速鱼(Alosa tanaica, Grimm, 1901)的生物学参数研究。
G. Țiganov, L. Oprea, V. Maximov
Azov shad ( Alosa tanaica , Grimm 1901) is a marine anadromous species, which migrates for spawning from sea into fresh water lakes and rivers. It winters in the sea and appears close to shore in spring when the water temperature reaches the value of approximately 6 degrees Celsius. The paper presents the biological parameters such as length and weight class structure, age, sex ratio and degree of maturation of the commercial fish Azov shad belonging  to  the  family Clupeidae .  The  investigations were  performed  during  2014 on  fish  caught  along  the entire Romanian coast  from  Sulina  to  Vama Veche. The study of the behaviour of Azov shad (reproduction, migration, feeding) is important to understand the impact on populations and to find sustainable solutions to manage these aspects. The preference for certain areas of distribution, for feeding and breeding was determined by analysing samples collected by passive fishing (shad gillnets set in all fishing points across the Romanian Coast). The main aim of this research is to identify the current state of Azov shad population along the Black Sea Coast considering that its biology and conservation are less known. Â
亚速鲥鱼(Alosa tanaica, Grimm 1901)是一种海洋溯河洄游物种,从海洋洄游到淡水湖泊和河流中产卵。它在海里过冬,在春天水温达到约6摄氏度时出现在海岸附近。本文介绍了亚速鱼(Azov shad) belonging to the Clupeidae科(family Clupeidae) . TheÂ调查were performed during 2014 on fish caught alongÂ罗马尼亚全鱼coast from Sulina to Vama Veche的体长、体重、级结构、年龄、性别比和成熟程度等生物学参数。研究亚速鲥鱼的行为(繁殖、迁徙、摄食)对于了解对种群的影响以及找到管理这些方面的可持续解决方案非常重要。通过分析被动捕鱼(在罗马尼亚海岸所有渔点设置刺网)收集的样本,确定了对某些分布区域、喂养和繁殖的偏好。考虑到其生物学和保护鲜为人知,本研究的主要目的是确定黑海沿岸亚速鲥鱼种群的现状。一个
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引用次数: 1
The Ash Content of the Main Muscle Groups and Edible Offal Collected From Hares (Lepus europaeus Pallas) 野兔(Lepus europaeus Pallas)主要肌群和可食用内脏的灰分含量
G. Frunză, C. Pop, P. Boisteanu
Abstract. The availability of hares (Lepus europaeus Pallas), unlike that of domestic rabbits, is restricted by hunting seasons. Rabbit meat, according to research from Spain, is a rich source of K, P, Fe and Mg, but poor in Na, being recommended for people with hypertension. The largest quantity of macro minerals studied in rabbit meat was determined for K and P, and the most abundant micro minerals were Zn, Fe, Cu and Mn. In hare meat, ash content is not sufficiently studied. The current low level of knowledge motivated the present study. The aim of this study was to establish the ash content for major muscle groups and edible offal of hares. The biological material used was collected from 49 hares with an average weight of 5.6 kg, at the age of reproductive maturity (adults: 11-12 months). Different muscle groups (the muscles Cervicalis, Intercostalis, Longissimus Dorsi, Psoas major, Triceps Brachi, Biceps femoris, Semimembranosus ) and the main edible offal (heart, liver, kidney) were sampled. The ash was determined by calcination (in Supertherm C311 oven calcination) at 550 0 C. The results obtained were interpreted statistically (arithmetic mean (X), standard deviation (s), variance (s 2 ) and coefficient of variation (V%) and the statistical significance of differences was tested using the ANOVA Single Factor algorithm (p>0.05; p<0.01; p<0.001). Ash content for the main muscle groups analysed varied from the lowest average values of 1.095% for the Intercostalis muscles, to the highest average values of 1.256%, for Triceps brachii muscles.
摘要野兔(Lepus europaeus Pallas)与家兔不同,受到狩猎季节的限制。根据西班牙的研究,兔肉富含钾、磷、铁和镁,但钠含量较低,建议高血压患者食用。家兔肉中微量元素以钾、磷含量最高,微量元素以锌、铁、铜、锰含量最高。兔肉中灰分含量的研究还不够充分。当前的低知识水平是本研究的动机。本研究的目的是确定兔主要肌肉群和可食用内脏的灰分含量。所使用的生物材料取自49只平均体重5.6 kg、生殖成熟期(成年:11-12个月)的野兔。采集不同肌肉群(颈肌、肋间肌、背最长肌、大腰肌、肱三头肌、股二头肌、半膜肌)和主要食用内脏(心、肝、肾)。测定灰分(Supertherm C311烘箱煅烧)温度为550℃,对所得结果进行统计学解释(算术平均值(X)、标准差(s)、方差(s 2)和变异系数(V%),并采用单因素方差分析(ANOVA单因素算法)检验差异的统计学显著性(p>0.05;p < 0.01;p < 0.001)。所分析的主要肌肉群的灰分含量从肋间肌的最低平均值1.095%到肱三头肌的最高平均值1.256%不等。
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引用次数: 0
Biodegradation study of some food packaging biopolymers based on PVA. 一些基于聚乙烯醇的食品包装生物聚合物的生物降解研究。
E. Tănase, E. Popa, M. Râpă, O. Popa, Ioana Popa
Abstract Polymers are a common choice as protective materials since they combine flexibility, variable sizes and shapes, relatively light weight, stability, resistance to breaking, barrier properties and perceived high-quality image with cost-effectiveness. Currently, mainly non-biodegradable petroleum-based synthetic polymers are used as packaging materials for foods, because of their availability, low cost and functionality. However, biopolymers can be made from renewable resources without the environmental issues of petroleum-based polymers and with the additional advantage of being available from renewable sources or as by-products or waste-products from the food and agriculture industries. The aim of this study was to test some food packaging biopolymers based on PVA. In this respect, some biopolymers for food packaging applications were subjected to biodegradation tests by covering the tested samples with soil. The samples were incubated in known temperature and humidity conditions. The experiment lasted 45 days, after that the samples were washed, weighed and the biodegradation degree was calculated. The obtained results shows that PVA is a promising material for food packaging usage, as it is made from renewable resources and it is environmentally friendly. Â
摘要聚合物是一种常用的防护材料,因为它们结合了灵活性、可变尺寸和形状、相对较轻的重量、稳定性、抗破碎性、屏障特性和具有成本效益的高质量图像。目前,主要使用不可生物降解的石油基合成聚合物作为食品包装材料,因为它们的易得性、低成本和功能性。然而,生物聚合物可以由可再生资源制成,没有石油基聚合物的环境问题,并且具有可再生资源或作为食品和农业工业的副产品或废物的额外优势。本研究的目的是测试一些基于聚乙烯醇的食品包装生物聚合物。在这方面,一些用于食品包装的生物聚合物通过用土壤覆盖测试样品进行了生物降解测试。样品在已知的温度和湿度条件下孵育。实验持续45 d,洗涤、称重并计算生物降解程度。结果表明,聚乙烯醇是一种很有前途的食品包装材料,因为它是由可再生资源制成的,并且对环境友好。一个
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引用次数: 15
Influence of temperature and freezing time on broiler chicken meat colour. 温度和冷冻时间对肉鸡肉色的影响。
M. Ciobanu, R. Lazăr, P. Boisteanu
Due to the fact that meat colour represents an important aspect for sensorial evaluation of any food product,research in the field of poultry meat processing regarding efficient methods for hoarding/storage on long term ofindustrially slaughtered poultry carcasses approaches a continuous finding/optimization of certain solutions foravoiding negative consequences due to oxidation, which could include colour loss and/or its modification.The current study aimed to evaluate the effects of three storage regimes differing by temperature and time(L = -14°C, 30 days; L = -16°C, 60 days; L = -18°C, 90 days) on three anatomical cut regions (breast, upper thighand lower thigh), to characterize the colour of broiler chicken meat. Objective description of colour for frozen anddefrosted chicken meat was realised through the CIEL*a*b* Cartesian coordinate system.Overall, the preservation method determines a higher luminosity at samples gathered from L batch chickencarcasses for breast and upper thigh musculature, and L for lower thigh musculature in comparison with thecounterparts from the other experimental batches
由于肉的颜色是任何食品感官评价的一个重要方面,在禽肉加工领域的研究中,关于对工业屠宰的家禽尸体进行长期贮藏/储存的有效方法,正在不断寻找/优化某些解决方案,以避免氧化造成的负面后果,其中可能包括颜色损失和/或其修改。目前的研究旨在评估三种不同温度和时间的储存制度的影响(L = -14°C, 30天;L = -16°C, 60天;L = -18°C, 90天)在三个解剖切割区域(乳房,大腿上部和大腿下部)上,以表征肉鸡肉的颜色。通过CIEL*a*b*笛卡尔坐标系实现了冷冻和解冻鸡肉颜色的客观描述。总体而言,与其他实验批次的样品相比,该保存方法确定了从L批次鸡胴体中收集的乳房和大腿上部肌肉组织样品和L批次鸡大腿下部肌肉组织样品具有更高的亮度
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引用次数: 2
Characterization of Microorganisms Isolated from Petroleum Hydrocarbon Polluted Soil 石油烃污染土壤中分离微生物的特性研究
A. Criste, Tabita, Mihaela, Marius, M. Niste, Nicodim, Fit, M. Mitrea
Bioremediation has received a great deal of attention, and bacteria isolated from polluted soil can be usedin that process. In this study, we performed an evaluation of the physiological groups of microorganisms fromsoil contaminated with petroleum. Bacterial strains were isolated from contaminated soil using the selectiveenrichment technique. Minimal Salt Media was used for serial dilutions to determine viable cell count. Thenumber of total viable cells and different types of microorganisms in the original sample was determined by serialdilution, agar plating procedure using selective media. The plates were incubated at 300C for 24-72 hours. Distinctcolonies growing on each plate were selected, and stored at freezing temperatures. The bacterial colonies werethen identified by Gram staining and biochemical tests. Following our research, it was observed that although thetotal microbial load of soil is relatively close in value, there are differences regarding the physiological group ofmicroorganisms. In the oil contaminated soil sample the largest group of microorganisms was the nitrous nitrifyingbacteria followed by nitrate bacteria. All bacterial strains that were isolated from soil samples contaminated withhydrocarbons but also the Pseudomonas putida and Bacillus subtillis strains can use diesel fuel as a food source.With the increase of diesel fuel concentration from culture medium, the majority of the bacterial strains that wereused in our experiments showed an increased value of absorbance. This fact suggests that these strains can be usedin bioremediation processes. Â
生物修复已受到广泛关注,从污染土壤中分离出的细菌可用于生物修复。在本研究中,我们对石油污染土壤中微生物的生理类群进行了评价。采用选择性富集技术从污染土壤中分离菌株。最小盐培养基连续稀释测定活细胞数。通过选择性培养基的连续稀释和琼脂电镀程序,确定原始样品中总活细胞和不同类型微生物的数量。300C孵育24-72小时。每个平板上生长的不同菌落被挑选出来,并保存在冷冻温度下。然后通过革兰氏染色和生化试验鉴定细菌菌落。通过我们的研究,我们发现虽然土壤的总微生物负荷值相对接近,但在微生物的生理类群上存在差异。在受石油污染的土壤样品中,微生物群以亚氮硝化细菌最多,其次是硝酸盐细菌。除恶臭假单胞菌和枯草芽孢杆菌菌株外,从被碳氢化合物污染的土壤样品中分离出的所有菌株都可以将柴油作为食物来源。随着培养基中柴油浓度的增加,我们实验中使用的大多数菌株的吸光度值都有所增加。这一事实表明,这些菌株可以用于生物修复过程。一个
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引用次数: 2
Quality Parameters for Commercial Royal Jelly 商品蜂王浆的质量参数
C. Mureșan, Liviu Alexandru MărghitaÈ, D. Dezmirean, Otilia BobiÈ, VictoriÈ›a Bonta, Iulia Zacharias, Rodica Mărgăoan, Claudia PaÈ™ca
Royal jelly has become a high-value commercial product and the standardization of this product is required to guarantee its quality on the market. The objective of the research activity was to pursue the chemical composition of commercial samples of Royal Jelly in Romania in order to propose standardization for this product. The physico-chemical composition of commercial Royal Jelly samples was analysed by determining quality parameters like: carbohydrates, lipids, proteins, 10-hydroxy-2-decenoic acid (10-HDA) and mineral elements. Carbohydrates analysis showed values between 3.4 % and 5.87 % for fructose, 4.12 % and 7.05 % for glucose, while for sucrose the values ranged between 0.95 % and 2.56 % (determined by HPLC-RI). The lipids content ranged between 1.85 % and 6.32 % (determined by the Soxhlet method). The protein values extended from 13.10 % (RJ2) to 17.04 % (RJ10) (the total protein content was determined by the Kjeldahl method). The values for the major fatty acid in Royal Jelly, 10-HDA, ranged between 1.35 % (RJ8) and 2.03 % (RJ10) (determined by high-performance liquid chromatography). The concentration of minerals varied between 3188.70 mg/kg and 4023.39 mg/kg (the concentration of minerals was measured using flame atomic absorption spectrometry). Potassium, followed by magnesium, sodium and calcium, occurs in the highest concentrations. The commercial Royal Jelly samples analysed presented variable physico-chemical characteristics that correspond with the values given by international quality standard proposals for Royal Jelly.
蜂王浆已成为一种高价值的商品,为保证其在市场上的质量,需要对其进行标准化。研究活动的目的是研究罗马尼亚蜂王浆商业样品的化学成分,以便为该产品提出标准化建议。通过测定碳水化合物、脂类、蛋白质、10-羟基-2-十烯酸(10-HDA)和矿物元素等质量参数,分析了商用蜂王浆样品的理化成分。碳水化合物分析显示果糖的含量在3.4% ~ 5.87%之间,葡萄糖的含量在4.12% ~ 7.05%之间,蔗糖的含量在0.95% ~ 2.56%之间(HPLC-RI测定)。脂质含量为1.85% ~ 6.32%(用索氏法测定)。蛋白质含量范围为13.10% (RJ2) ~ 17.04% (RJ10)(总蛋白质含量采用凯氏定氮法测定)。蜂王浆中主要脂肪酸10-HDA含量在1.35% (RJ8) ~ 2.03% (RJ10)之间(高效液相色谱法测定)。矿物浓度在3188.70 ~ 4023.39 mg/kg之间变化(矿物浓度采用火焰原子吸收光谱法测定)。钾的浓度最高,其次是镁、钠和钙。所分析的商业蜂王浆样品呈现出与蜂王浆国际质量标准建议值相对应的可变物理化学特征。
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引用次数: 4
Pharmaceuticals and Dietary Supplements Extracted from Mare’s Milk 从马奶中提取的药品和膳食补充剂
A. Stuparu, T. Oroian, C. E. Strugariu
Mare’s milk is similar to human breast milk and has valuable therapeutic properties. For this reason, Europeans are increasingly interested in discovering its benefits and how the chemical composition of horse milk differs from that of other species. This interest is reflected in the number of new farms selling mare’s milk around the world as this milk is the most similar to human milk. Mare’s milk is considered to be highly digestible, rich in essential nutrients and whey protein, which makes it very suitable as a substitute for bovine milk in paediatric diets. During the period of lactation, mammal's milk composition is subject to rapid changes in macro- and micro-elements, as well as in the quantity and quality of proteins, lipids and saccharides, being ideal food for infants. The primary use of mare’s milk has been the rearing of foals, but recently, due to the similarity of its chemical composition to that of human milk, it has raised particular interest and some experiments have been done to apply it as a raw material for the preparation of special food products for human consumption. Mare’s milk products are very common in Russia and Central Asia throughout Mongolia. In the 19 th century, some Russian scientists explored its therapeutic properties and then in 1859 they founded the first sanatorium where treatments with mare’s milk could be made under medical control. Due to its potential health-promoting characteristics, in the Western countries, interest in mare's milk has lately increased. Â
mare的乳汁类似于人类母乳,具有宝贵的治疗特性。由于这个原因,欧洲人越来越有兴趣发现它的好处,以及马奶的化学成分与其他物种的不同之处。这种兴趣反映在世界各地销售mare牛奶的新农场的数量上,因为这种牛奶与人类的牛奶最相似。mare的牛奶被认为是高度可消化的,富含必需营养素和乳清蛋白,这使得它非常适合作为儿科饮食中牛奶的替代品。哺乳动物的乳成分在哺乳期间,其宏量元素和微量元素以及蛋白质、脂质和糖类的数量和质量都发生了快速变化,是婴儿的理想食物。马乳的主要用途是饲养马驹,但最近,由于其化学成分与人乳相似,它引起了特别的兴趣,并且已经做了一些实验,将其作为制备供人类食用的特殊食品的原料。mare的奶制品在俄罗斯和中亚蒙古非常普遍。在19世纪,一些俄罗斯科学家探索了它的治疗特性,然后在1859年,他们建立了第一家疗养院,在那里可以在医学控制下用mare牛奶进行治疗。由于其潜在的促进健康的特点,在西方国家,对马奶的兴趣最近有所增加。一个
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引用次数: 7
Appreciation of the Mammary Gland Health Status Based on the Quality Traits of Milk Obtained from Friesian - Black Spotted Breed 基于黑斑黑斑犬乳品质性状的乳腺健康状况评价
G. Onaciu, E. Jurco, L. Ognean
One of the main objectives in dairy cows exploitation is to guarantee food security and safety of the population, a desideratum achieved through better milk quality and better control of production in all aspects imposed by the health and welfare of animals from which milk is obtained. The purpose of this sciencific paper is to highlight the status of mammary gland health in a population of Friesian-Black Spotted breed based on the main traits of milk quality, with particular reference to somatic cell counts as the main indicator of subclinical mastitis. The research was conducted in 2014 within the farm S.C. “Modern Farm” S.R.L., located in Jucu village. The individual analysis of Holstein Friesian milked cows was performed, the main indicators of milk production were analysed both quantitatively, based on production obtained daily, and especially qualitatively, based on seven controls with individual sampling from all milked cows at the date of control. Qualitative analysis of 1,391 milk samples highlighted the fact that the somatic cell count in milk obtained from cattle included in this study  recorded an average value of 390.54 ± 11.16 cells/ml x 10 3 with the variability between controls of 314.94 ± 28.93 and 482.36 ± 91.12 cells/ml x 10 3 . Regarding the fat content, the average values was 4.19%, 3.63% for protein content, a pH value of 6.56 and the values of  urea was 20.09 mg/dl. Individual analysis also revealed some cows with subclinical mastitis, which were milked separately, verified and monitored supplementarily in order to reestablish udder health and to prevent the occurrence of clinical mastitis, which can compromise the mammary gland. Â
奶牛开发的主要目标之一是保证人口的食品安全和安全,这是通过更好的牛奶质量和更好的生产控制来实现的,这些控制是由获得牛奶的动物的健康和福利所施加的。这篇科学论文的目的是根据乳质的主要特征,特别提到体细胞计数作为亚临床乳腺炎的主要指标,来强调弗里西亚-黑斑犬群体的乳腺健康状况。该研究于2014年在位于juu村的农场S.C. €œModern farm€s.r.l.进行。对荷斯坦奶牛进行个体分析,对产奶量的主要指标进行定量分析(基于每日获得的产奶量),特别是定性分析(基于7个对照,在对照日对所有奶牛进行个体采样)。对1391份牛奶样品的定性分析表明,本研究中奶牛的体细胞计数Â的平均值为390.54 ±11.16个细胞/ml × 103,对照组为314.94 ±28.93和482.36 ±91.12个细胞/ml × 103。脂肪含量平均值为4.19%,蛋白质含量平均值为3.63%,pH值为6.56,Â尿素含量为20.09 mg/dl。个别分析还发现了一些患有亚临床乳腺炎的奶牛,这些奶牛被单独挤奶,并进行了验证和补充监测,以恢复乳房健康,防止可能损害乳腺的临床乳腺炎的发生。一个
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引用次数: 2
Total Content of Polyphenols and Antioxidant Activity of Different Melliferous Plants 不同蜜科植物多酚总含量及抗氧化活性研究
C. Pașca, L. Mǎrghitaş, O. Bobiș, D. Dezmirean, Rodica Mărgăoan, C. Mureșan
In this study polyphenols content and antioxidant activity of melliferous plants for the following: mint ( Mentha pulegium) , burdock ( Arctium lappa), comfrey (Symphytum officinale), plantain (Pantago lanceolata), thyme (Thymus vulgaris), sage (Salvia officinalis), marigold (Calendula officinalis) , small marshmallow (Althaea officinalis), echinacea (Echinaceea angustifolia) and black popular (Populus nigra) were investigated, using two different extraction methods. High content of polyphenols and flavones were extracted from Populus nigra , with an average of both extractions 23.14 mg GAE/g and 78.07 mg QE/g flavones. Among the studied plants, Arctium lappa registered the highest antioxidant activity (0.129 mmol Trolox/mL) in alcoholic extract and Echinaceea angustifolia with a value of 0.122 mmol Trolox/mL in aqueous extract. The lowest values were recorded for the antioxidant activity of Althaea officinalis (alcoholic extract) and Arctium lappa (aqueous extract). The results show that Arctium lappa, Echinaceea angustifolia and Populus nigra can be considered melliferous plants for their high biologically active compounds potential and bee products (honey and pollen) that having the composition of these plants will have high antioxidant and antibacterial properties.
以薄荷(Mentha pulegium)、牛蒡(Arctium lappa)、紫草(Symphytum officinale)、车前草(Pantago lanceolata)、百里香(Thymus vulgaris)、鼠尾草(Salvia officinalis)、万寿菊(Calendula officinalis)、小棉花糖(Althaea officinalis)、紫锥菊(Echinaceea angustifolia)和黑杨树(Populus nigra)为研究原料,采用两种不同的提取方法对其多酚含量和抗氧化活性进行了研究。从黑杨中提取的黄酮和多酚含量较高,平均黄酮含量为23.14 mg GAE/g,平均黄酮含量为78.07 mg QE/g。其中,牛蒡醇提物的抗氧化活性最高(0.129 mmol Trolox/mL),刺锥菊水提物的抗氧化活性最高(0.122 mmol Trolox/mL)。牛蒡水提物和杜鹃花醇提物的抗氧化活性最低。结果表明,牛蒡、紫锥菊和黑胡杨具有较高的生物活性化合物潜力,且含有这些植物成分的蜂产品(蜂蜜和花粉)具有较高的抗氧化和抗菌性能。
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引用次数: 15
New MTCYB haplotypes in Romanian buffalo. 罗马尼亚水牛新的MTCYB单倍型。
C. Coroian, A. Coroian, V. Mireșan, M. Șuteu, C. Lațiu, C. Răducu
The Romanian Buffalo Breed (RBB), established in 1987, originated from local river buffalo populations highly adapted to the low-input breeding condition. Cross-breeding with Asian breeds was recently used for genetic improvement in milk production. In the last 25 years the species has dramatically decreased. Over 90% of livestock has been sold and never replaced. The population is now mainly maintained in subsistence farms. This study aims to assess the genetic diversity within the remaining population and to verify links with improved breeds supposedly introgressed in RBB. To assess the genetic diversity of RBB and ascertain possible phylogenetic relations with other buffalo breeds, we sequenced the entire cytochrome B gene (MTCYB) in a sample population. Blood samples were collected from randomly selected 52 unrelated individuals from various locations in Transylvania. A DNA fragment containing the entire MTCYB gene (1140bp) was amplified by PCR directly from whole blood (1μl). The amplicons were sequenced using two pairs of primers. The sequences were subsequently used for genetic diversity assessments. Analysis of the sequences led to the identification of five novel MTCYB haplotypes, uploaded in GenBank with the following accession numbers: JQ241279; JQ241280; JQ241281; JQ241282; JQ241283. In the sample population, the most frequently identified haplotypes were haplotype 2 (EF409940.1) and haplotype 3 (EF409941.1), previously reported in other buffalo populations, mainly from Asia, confirming the Asiatic origins of RBB. Sequence comparisons have revealed that RBB is mainly related with Indian breeds. Despite the numerical decline of RBB, through correct breeding schemes the breed maintained a good level of genetic diversity. Our analysis confirms RBB’s Asiatic origins. Presence of new haplotypes may also reflect that this population is isolated from a geographical and reproductive point of view.
罗马尼亚水牛品种(RBB)成立于1987年,起源于高度适应低投入育种条件的当地河水牛种群。与亚洲品种杂交最近被用于牛奶生产的遗传改良。在过去的25年里,这个物种急剧减少。超过90%的牲畜被出售,从未被替换过。人口现在主要维持在自给农场。本研究旨在评估剩余种群的遗传多样性,并验证与RBB中可能渗入的改良品种之间的联系。为了评估RBB的遗传多样性,并确定与其他水牛品种可能的系统发育关系,我们对一个样本群体的整个细胞色素B基因(MTCYB)进行了测序。血液样本是从特兰西瓦尼亚不同地点随机选择的52名无血缘关系的人身上收集的。直接从全血(1μl)中扩增出含有MTCYB全基因的DNA片段(1140bp)。利用两对引物对扩增子进行测序。这些序列随后被用于遗传多样性评估。通过对序列的分析,鉴定出5个新的MTCYB单倍型,并将其上传至GenBank,登录号为:JQ241279;JQ241280;JQ241281;JQ241282;JQ241283。在样本群体中,最常见的单倍型是单倍型2 (EF409940.1)和单倍型3 (EF409941.1),之前在其他水牛群体中报道过,主要来自亚洲,证实了RBB的亚洲起源。序列比较显示RBB主要与印度品种有关。尽管RBB数量下降,但通过正确的育种方案,该品种保持了良好的遗传多样性水平。我们的分析证实了RBB的亚洲起源。新的单倍型的出现也可能反映了这个种群从地理和繁殖的角度来看是孤立的。
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引用次数: 2
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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