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Deposition and adhesion of spores of Fusarium culmorum on hydrilla. 镰刀菌孢子在水螅上的沉积和粘附。
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501193
M. Smither-Kopperl, R. Charudattan, R. Berger
Fusarium culmorum is a pathogen of the submerged aquatic weed Hydrilla verticillata. The deposition and attachment of macroconidia and chlamydospores of F. culmorum in water was investigated using assays with glass slides and hydrilla shoots. The number of spores deposited on leaves along the length of hydrilla shoots in test tubes did not vary significantly with depth. Macroconidia and chlamydospores deposited on the leaf did not become attached to the surface for several hours. Two-week-old macroconidia required 3 h for attachment and 6-week-old chlamydospores required 7 h. Pretreatment of macroconidia in hydrilla extract induced production of an adhesive substance from the tips of the macroconidia, which attached to glass slides and hydrilla leaves. The adhesive substance was induced after incubation for l min in hydrilla extract, and the adhesive capacity declined after 20 min of incubation. Macroconidia that adhered to glass slides began to break free after 3 h. Co-incubation of macroconidia in hydri...
镰刀菌是水草水螅的一种病原菌。采用玻片法和水螅芽法,研究了镰刀菌大孢子和衣原体孢子在水中的沉积和附着。在试管中,沿水螅芽的长度沉积在叶片上的孢子数随深度的变化不显著。大分生孢子和衣孢子沉积在叶片上几个小时后才附着在表面。两周大的分生孢子需要3小时的附着时间,6周大的衣孢子需要7小时的附着时间。在水螅提取物中预处理大分生孢子,诱导大分生孢子的尖端产生一种粘附物质,附着在玻璃片和水螅叶片上。水螅浸膏培养1 min后产生黏附物质,培养20 min后黏附能力下降。粘附在玻片上的大分生孢子在3 h后开始脱落。
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引用次数: 11
Classical and molecular approaches for managing nematodes affecting potato
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501185
B. Brodie
Potato cyst (Globodera spp.), root-knot (Meloidogyne spp.), and root lesion (Pratylenchus spp.) nematodes are the most economically important nematodes of potatoes in temperate climates. Potato cyst nematodes occur worldwide and include two species, Globodera pallida (the white cyst nematode) and Globodera rostochiensis (the golden nematode), each composed of several pathotypes. The gene H1 which confers a high level of resistance to the golden nematode pathotype Rol, has been bred into several potato cultivars that are grown worldwide. Mapping of the H) gene has led to the development of a molecular marker to screen segregating populations for resistance to the golden nematode and to monitor the use of resistant cultivars in statutory control programs. Pathotype Ro2 of the golden nematode that overcomes H1-mediated resistance was recently discovered in New York State in the United States, and a high level of resistance to this pathotype has been identified. Several sources of resistance to G. pallida are...
马铃薯囊肿线虫(Globodera spp.)、根结线虫(Meloidogyne spp.)和根损线虫(Pratylenchus spp.)是温带地区马铃薯最重要的经济线虫。马铃薯囊肿线虫分布在世界各地,包括两种,白色囊肿线虫(Globodera pallida)和金色线虫(Globodera rostochiensis),每一种都由几种病型组成。对金黄色线虫致病型Rol具有高水平抗性的H1基因已被培育到世界各地种植的几个马铃薯品种中。H)基因的定位导致了一种分子标记的发展,用于筛选对金线虫抗性的分离群体,并在法定控制计划中监测抗性品种的使用。最近在美国纽约州发现了克服h1介导的抗性的金线虫的病原型Ro2,并已确定对该病原型的高水平抗性。对苍白菌的几种抗性来源是……
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引用次数: 14
Response of solanaceous cultivated plants and weed species to inoculation with A1 or A2 mating type strains of Phytophthora infestans 茄类栽培植物和杂草对接种A1或A2交配型病原菌的反应
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501195
H. W. Platt
Hypersensitive response symptoms of late blight were induced when sporangia of Phytophthora infestans were inoculated on discs of excised leaf tissues of black nightshade, eggplant, green pepper, and tobacco. No symptoms due to disease or hypersensitive response were observed in stem piece tissues of these plant species, suggesting that these four are nonhosts for late blight. The Al (US-1), the A2 (US-8), and the more recent Al mating type (US-ll) strains infected both leaf and stem tissues of climbing and hairy nightshade, potato, and tomato but only leaf tissues of petunia. This is the first report of the new strains of P. infestans causing late blight symptoms on climbing nightshade and petunia in Canada. Based on infection levels, infection rates, and spore production, the US-8 and US-ll genotypes were similar and both were more aggressive than the US-1 genotype. Disease severity and sporulation were greater on potato inoculated with US-1 than for the other host plants and were the least on petunia. ...
将疫霉孢子囊接种于茄、茄子、青椒和烟草的离体叶组织盘上,可诱发晚疫病的超敏反应症状。在这些植物的茎片组织中未观察到因疾病或过敏反应引起的症状,表明这四种植物不是晚疫病的寄主。Al (US-1)、A2 (US-8)和最近的Al交配型(US-1)菌株侵染攀缘、毛茄、马铃薯和番茄的叶和茎组织,但只侵染矮牵牛的叶组织。这是加拿大首次报道引起攀缘龙葵和矮牵牛晚疫病症状的新菌株。基于感染水平、感染率和孢子产量,US-8和US-1基因型相似,且都比US-1基因型更具侵袭性。接种US-1的马铃薯的病害严重程度和产孢量均大于其他寄主植物,而矮牵牛. ...的病害严重程度和产孢量最少
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引用次数: 33
A multiple disease resistant potato clone developed with classical breeding methodology 利用经典育种方法获得了一个马铃薯多重抗病无性系
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501183
A. Murphy, H. Jong, K. Proudfoot
Genetic plant resistance is one strategy for minimizing the effects of disease. Identification and improvements in inherent resistance have been longstanding objectives of Agriculture and Agri-Food Canada's potato breeding program at Fredericton. The potato as known in North America is a tetraploid, vegetatively propagated crop that is susceptible to a wide range of diseases and pests. Disease resistance is one of many attributes, including adaptation, consumer quality, and appearance, that must be combined in a new cultivar before it is accepted and grown on any scale. Classical breeding programs have sought and transferred sources of resistance from cultivated and wild relatives of the potato. This paper describes the approaches, the methods, and some of the hurdles encountered in the process. The example of clone F87084, which has resistances to several different diseases, will be used to portray the cooperative efforts that will culminate in this selection's release as adapted, disease-resistant germp...
植物遗传抗性是减少病害影响的一种策略。鉴定和改进固有抗性一直是加拿大农业和农业食品部在弗雷德里克顿的马铃薯育种项目的长期目标。马铃薯在北美是一种四倍体,无性繁殖的作物,易受多种病虫害的影响。抗病性是许多属性之一,包括适应性、消费者质量和外观,在新品种被接受和大规模种植之前必须结合在一起。传统的育种计划已经从马铃薯的栽培和野生近缘种中寻找并转移了抗性来源。本文描述了方法、方法以及在此过程中遇到的一些障碍。以克隆体F87084为例,它对几种不同的疾病具有抗性,将被用来描述这种合作努力,最终将这种选择作为适应的、抗病的细菌发布。
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引用次数: 16
Survival of leaf spot pathogens on crop residues of wheat and barley in Saskatchewan. 萨斯喀彻温省小麦和大麦叶斑病病原菌在作物残茬上的存活。
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501208
L. Duczek, K. Sutherland, S. Reed, K. Bailey, G. Lafond
Pycnidia of Stagonospora nodorum, and pseudothecia of Phaeosphaeria nodorum and Pyrenophora triticirepentis occurred in greater numbers after one overwintering period on residues of wheat and barley stored on the surface of the soil than on residues from the previous fall. Conidial numbers of Bipolaris sorokiniana and Drechslera teres on residues of wheat and barley, and pseudothecia of Pyrenophora teres on residues of barley decreased over one winter but these pathogens continued to sporulate at low levels after two winters. With residue from the soil surface, Drechslera teres and B. sorokiniana conidia occurred in greater numbers on lower parts of the plants. Drechslera teres occurred primarily on leaf and sheath areas and B. sorokiniana, on sheath and stem areas, while P. teres sporulated mainly on stems. Numbers of conidia of D. teres may have declined because the pathogen occurred mainly on leaves, which disintegrated after one winter. The wheat pathogen Stagonospora nodorum was able to sporulate on ...
一个越冬期后,土壤表面储存的小麦和大麦残余物上出现的穗状芽孢杆菌、穗状芽孢杆菌和三萜芽孢杆菌的假毛虫数量多于前一个秋季的残余物。小麦和大麦残余物上的双极虫孢子数和圆锥虫孢子数,以及大麦残余物上的圆锥虫孢子数在一个冬季内减少,但在两个冬季后,这些病原菌孢子数仍保持在较低水平。在土壤表面残留的情况下,球茎螺旋体和白螺旋体分生孢子在植物下部的数量较多。圆螺旋体主要发生在叶片和鞘区,白螺旋体主要发生在鞘和茎区,圆螺旋体主要在茎上产孢。由于病原菌主要寄生在叶片上,经过一个冬季后叶片就会崩解,导致了球孢分生孢子数量的减少。小麦病原菌芽孢菌(Stagonospora nodorum)在…
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引用次数: 59
The identification of a cleaved amplified polymorphic sequence (CAPS) marker for the flax rust resistance gene M3 亚麻抗锈病基因M3的裂解扩增多态性序列(CAPS)标记
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501211
G. Hausner, K. Rashid, E. Kenaschuk, J. Procunier
A cleaved amplified polymorphic sequence (CAPS) marker has been developed for the flax rust resistance gene M3. This molecular marker was identified in a rust differential for M3 by screening primer sets, whose sequences were based on the nucleotide sequence of the M gene. One primer set was identified that co-amplified, along with other related sequences, a 2.9-kb DNA segment of M3 or an M-like gene tightly linked to the M3 locus. Restriction fragment analysis revealed several unique DNA fragments that were linked to the presence of the M3 gene in a F2 segregating population. This molecular marker along with recently developed markers for the L2, L6, L9, and L11 alleles will be useful for marker-assisted selection in breeding programs for obtaining multigenic resistance against flax rust in Canadian flax cultivars.
建立了亚麻抗锈病基因M3的CAPS标记。根据M基因的核苷酸序列筛选引物,在M3锈病鉴别中鉴定出该分子标记。其中一组引物与其他相关序列一起扩增了M3的2.9 kb DNA片段或与M3位点紧密相连的m样基因。限制性内切酶片段分析显示,在F2分离群体中,有几个独特的DNA片段与M3基因的存在有关。该分子标记与最近开发的L2、L6、L9和L11等位基因标记将有助于标记辅助选择,以获得加拿大亚麻品种的多基因抗锈病育种计划。
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引用次数: 8
Effect of inoculum type and anastomosis group of Rhizoctonia solani causing wirestem of cabbage seedlings in a controlled environment 受控环境下番茄根丝核菌接种类型和吻合群对白菜幼苗生茎的影响
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501201
P. A. Rollins, A. Keinath, M. Farnham
Three-week-old seedlings of cabbage cv. Bravo were inoculated with either infested cornmeal-sand cultures (9.1 x 104 colony-forming units per kg soil) or selerotia (50 per kg soil) of isolates of Rhizoctonia solani anastomosis group (AG) 2-1 (five isolates) or AG4 (five isolates). Wirestem incidence (percentage of diseased and dead plants), severity on hypocotyls and roots (rated on a l-lO scale), and plant fresh weight were assessed two weeks after inoculation. Wirestem incidence and severity were greater and plant weight was lower with cornmeal-sand inoculum than with sclerotial inoculum. As a group, isolates belonging to AG4 were significantly more virulent than those of AG2-1 under conditions of this study. Within each AG, isolates could be separated based on virulence with either inoculum type. Use of sclerotial inoculum of a virulent isolate of R. solani AG4 or AG2-1 would provide the most practical means to screen Brassica oleracea germplasm.
三周大白菜幼苗。Bravo接种了侵染的玉米砂培养物(每公斤土壤9.1 × 104个菌落形成单位)或葡萄根丝核菌吻合组(AG) 2-1(5株)或AG4(5株)分离株的selerotia(每公斤土壤50株)。接种两周后,对茎干发生率(患病和死亡植株的百分比)、下胚轴和根系的严重程度(按l-lO量表评定)和植株鲜重进行评估。与菌核接种相比,玉米砂接种的茎干发生率和严重程度更高,植株重量更低。作为一个群体,在本研究条件下,属于AG4的分离株的毒力明显高于AG2-1。在每个菌群中,菌株可根据任一种接种类型的毒力进行分离。利用番茄枯萎病菌AG4或AG2-1强毒株的菌核接种是筛选甘蓝种质资源最实用的方法。
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引用次数: 13
USE OF ALLOZYME MARKERS TO DETERMINE GENOTYPES OF PHYTOPHTHORA INFESTANS IN CANADA 用同工酶标记测定加拿大疫霉菌基因型
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501205
R. Peters, H. W. Platt, R. Hall
A comparison of allozyme banding patterns at two loci, for glucose-6-phosphate isomerase (Gpi) and peptidase (Pep), with markers for mating type, metalaxyl sensitivity, and cultural morphology revealed eight distinct genotypes among 726 isolates of Phytophthora infestans collected in Canada between 1994 and 1996. Banding patterns for allozymes of the Gpi locus alone proved to be adequate for distinguishing seven of the genotypes. Five of these genotypes (US-l, US-6, US-7, US-8, and gll (or US-ll)) were comparable to genotypes described in the United States. The US-l genotype (Al mating type, metalaxyl-sensitive) was recovered commonly (41% frequency) throughout Canada (outside the province of British Columbia) during 1994. By 1996, the US-l genotype was no longer recovered from any samples of potato or tomato tissue taken from across Canada. The US-8 genotype (A2 mating type, metalaxyl-insensitive) dominated populations of the pathogen in Ontario, Quebec, and New Brunswick during 1994 and by 1996, the US-...
对1994年至1996年在加拿大采集的726株病原菌进行了葡萄糖-6-磷酸异构酶(Gpi)和肽酶(Pep)两个基因座的同工酶带型与配种型、甲轴酯敏感性和培养形态标记的比较,发现8种不同的基因型。仅Gpi位点的同工酶带型就足以区分7个基因型。其中5个基因型(us - 1、US-6、US-7、US-8和gll(或us - 1))与美国描述的基因型相似。1994年在加拿大(除不列颠哥伦比亚省外)普遍发现us - 1基因型(Al交配型,甲酰基敏感)(41%频率)。到1996年,美国- 1基因型不再从加拿大各地的任何马铃薯或番茄组织样本中恢复。US-8基因型(A2交配型,甲酰基不敏感)在1994年和1996年在安大略省、魁北克省和新不伦瑞克省占主导地位。
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引用次数: 27
Inheritance of loose smut (Ustilago tritici) resistance in two hexaploid wheat (Triticum aestivum) lines 两个六倍体小麦品系抗松黑穗病的遗传研究
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501209
R. Knox, M. R. Fernandez, A. Brûlé-Babel, R. M. Pauw
The genetics of resistance in wheat (Triticum aestivum L.) to loose smut caused by Ustilago tritici (Pers.) Rostr. is not well understood. In this study the inheritance of loose smut resistance was studied in two androgenetically derived doubled haploid populations and two inbred random head-to-row populations. The loose smut resistant experimental wheat line HY377 was crossed with the loose smut susceptible line L8474-D1 and the resistant line SC8021V2. Androgenetically derived doubled haploid populations and F4-derived random inbred line populations were produced from each of the crosses HY377/L8474-D1 and HY377/SC8021V2. Lines derived from each cross were grown in the growth room and inoculated with individual and a mixture of races of loose smut. The segregation of resistance between HY377 and L8474-D1 to races T2, T10, T19, and T39 in both doubled haploid and random inbred line populations fit the expected ratio for a single major gene. The same populations inoculated to race T15 showed a different d...
小麦(Triticum aestivum L.)对黑穗病黑穗病(Ustilago tritici, Pers.)抗性的遗传Rostr。不是很好理解。本研究在两个雄性遗传来源的双单倍体群体和两个随机自交的头对行群体中研究了松散黑穗病抗性的遗传。将抗松黑穗病试验小麦品系HY377与松黑穗病敏感品系L8474-D1和抗松黑穗病品系SC8021V2杂交。杂交组合HY377/L8474-D1和HY377/SC8021V2分别获得双单倍体群体和f4随机自交系群体。从每个杂交中获得的系在生长室内生长,用单个和松散黑穗病种的混合物接种。在双单倍体和随机自交系群体中,HY377和L8474-D1对T2、T10、T19和T39小种的抗性分离符合单个主基因的预期比例。同样的群体接种到T15后表现出不同的免疫反应。
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引用次数: 15
INFLUENCE OF GROUND COVER ON DEVELOPMENT OF PHYTOPHTHORA CROWN AND ROOT ROT OF APPLE TREES 地被覆盖对苹果树疫霉冠腐病和根腐病发育的影响
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501199
R. Utkhede, E. Hogue
The influence of ground cover with fall rye, perennial rye grass, oats, canola, quack grass, and timothy grass on percent mortality and disease severity ratings of phytophthora crown and root rot of apple trees was determined over five years under orchard conditions in the Okanagan Valley of British Columbia. Fall rye and perennial rye grass significantly (P = 0.05) reduced the disease compared with the hand-weeded control. There were no significant differences in the percent tree mortality among the timothy grass, quack grass, oats, canola, and the hand-weeded control treatments. These results indicate that use of fall rye or perennial rye grass as a ground cover will reduce the incidence of phytophthora crown and root rot of apple trees planted in sandy soils of nurseries or orchards where the disease is present and conditions are sufficiently wet.
在不列颠哥伦比亚省奥肯那根山谷的果园条件下,测定了在5年的时间里,秋黑麦、多年生黑麦草、燕麦、油菜籽、呱呱叫草和蒂莫西草对苹果树疫霉病死率和根腐病严重程度等级的影响。秋季黑麦和多年生黑麦与手除草对照相比显著降低了病害(P = 0.05)。山楂草、庸医草、燕麦、油菜籽与手除草对照处理的树木死亡率无显著差异。这些结果表明,使用秋季黑麦或多年生黑麦草作为地面覆盖物,可以减少在苗圃或果园的沙质土壤中种植的苹果树的疫霉冠腐病和根腐病的发病率,并且条件足够潮湿。
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引用次数: 3
期刊
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie
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