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Comparative susceptibility of subalpine firs to Douglas-fir dwarf mistletoe 亚高山冷杉对道格拉斯冷杉矮槲寄生的比较易感性
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600132
R. Mathiasen
Abstract Subalpine fir (Abies Lasiocarpa (Hook.) Nutt.) and Rocky Mountain subalpine fir (Abies bifolia A. Murr.) are more susceptible to infection by Douglas-fir dwarf mistletoe (Arceuthobium douglasii Engelm.) than previously reported. Temporary circular plots (6-m radius) were established around dominant, severely infected Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco). Within plots, species, diameter at 1.37 m above the ground, and dwarf mistletoe rating (6-class system) were determined for each live tree over 1.37 m in height. Sixty-six percent and 15% of the subalpine fir and Rocky Mountain subalpine fir, respectively, sampled near large, severely infected Douglas-fir were infected. In contrast, over 90% of the Douglas-fir in the same plots were infected. Based on these results, subalpine fir is classified as a secondary host of Douglas-fir dwarf mistletoe and Rocky Mountain subalpine fir is classified as an occasional host. Variation in the infection of subalpine fir by Douglas-fir dwarf mistle...
亚高山冷杉(Abies Lasiocarpa (Hook.))Nutt.)和落基山亚高山冷杉(Abies bifolia A. Murr.)比以前报道的更容易受到道格拉斯冷杉矮槲寄生(Arceuthobium douglasii Engelm.)的感染。在显性、严重感染的孟氏假杉树(pseuddosuga menziesii, Mirb.)周围建立临时圆形样地(半径6 m)。弗朗哥)。在样地内,对每棵高度超过1.37 m的活树进行了种类、离地直径和矮槲寄生等级(6级制)的测定。亚高山冷杉和落基山亚高山冷杉分别有66%和15%的样本在大型、严重感染的道格拉斯冷杉附近被感染。相比之下,在同一地块,超过90%的道格拉斯冷杉被感染。在此基础上,将亚高山冷杉划分为道格拉斯冷杉矮槲寄生的次生寄主,将落基山亚高山冷杉划分为偶发寄主。矮槲寄生对亚高山冷杉侵染的影响
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引用次数: 3
The verticillium threat to canada’s major oilseed crop: canola 黄萎病对加拿大主要油料作物油菜籽的威胁
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600114
J. B. Heale, V. Karapapa
Abstract Verticillium wilt of winter oilseed rape (Brassica napus subsp. oleifera), reported previously as being caused by Verticillium dahliae Klebahn, or V. dahliae var. longisporum Stark, 1961, has caused serious economic losses in Sweden since 1960 and is now widespread in Europe. We have recently fully characterized and reclassified this host-adapted, near-diploid pathogen at the species level: Verticillium longisporum comb. nov. (Karapapa, Bainbridge, and Heale. 1997. Mycol. Res. 101: 1281–1294, and Karapapa, Bainbridge, and Heale. 7th International Verticillium Symposium, Cape Sounion, Athens, Greece, Oct. 1997. Abstr. p. 12). This pathogen causes yield losses, both in oilseed rape and related Brassica, in Europe, Asia, and Japan, but has not yet been reported on the U.K. oilseed rape crop or on the Canadian canola crop. Verticillium wilt in cauliflower in California, reported in 1994–1995 as involving a relatively large-spored V. dahliae with high nuclear DNA content, may be the first evidence of ...
摘要/ Abstract摘要:冬季油菜黄萎病的研究。先前报道的由大百合黄萎病(Verticillium dahliae Klebahn,或V. dahliae var. longisporum Stark, 1961)引起的油油病,自1960年以来在瑞典造成了严重的经济损失,现在在欧洲广泛传播。我们最近在物种水平上完全表征和重新分类了这种适应宿主的近二二体病原体:长孢黄萎病梳状体。11月(Karapapa, Bainbridge和Heale, 1997)。Mycol。Res. 101: 1281-1294,和Karapapa, Bainbridge, and Heale.第七届国际黄萎病研讨会,苏尼翁角,希腊雅典,1997年10月。Abstr。12页)。这种病原菌在欧洲、亚洲和日本的油菜和相关的芸苔属植物中造成产量损失,但在英国的油菜作物或加拿大的油菜籽作物中尚未报道。1994-1995年报道的加州花椰菜黄萎病涉及一种核DNA含量高、孢子较大的dahliae,这可能是黄萎病的第一个证据。
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引用次数: 76
Control of anthracnose in lentil using partial resistance and fungicide applications 应用部分抗性和杀菌剂防治扁豆炭疽病
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600116
G. Chongo, C. C. Bernier, L. Buchwaldt
Abstract Partial resistance and fungicide applications were evaluated in 1995 and 1996 for control of anthracnose in lentil (Lens culinaris) caused by Colletotrichum truncatum. Line 458-57 (partially resistant) and cv. Eston (susceptible) were planted in fields with a history of low, partial, and high anthracnose pressures. Unsprayed plots were compared with plots receiving a single application of either I or 2 kg a. i./ha of chlorothalonil at the 10- to 12-node stage, and two applications of 1 kg a.i./ha at the 10- to 12-node stage and to days later. Anthracnose severity in unsprayed plots was highest (78% for cv. Eston and 62% for 458-57) at high disease pressure, intermediate (62% for cv. Eston and 46% for 458-57) at partial disease pressure, and lowest (26% for cv. Eston and 15% for 458-57) at low disease pressure. Yield losses in unsprayed plots ranged from 26 to 57% in cv. Eston and 20 to 28% in 458-57, with the most significant losses occurring in cv. Eston at high disease pressure. Applications of...
摘要1995年和1996年对扁豆(Lens culinaris)炭疽病进行了部分抗性评价和杀菌剂应用评价。第458-57行(部分抗性)和cv。将Eston(易感)种植在具有低、部分和高炭疽病压力历史的田地中。将未喷施的小区与在10- 12节期单次施用1或2公斤a.i./公顷的小区进行比较,并在10- 12节期和之后几天两次施用1公斤a.i./公顷的小区进行比较。未喷药地块炭疽病严重程度最高(78%)。Eston和458-57的62%)在高疾病压力下,中等(cv为62%)。Eston和458-57的46%)的部分疾病压力最低(cv为26%)。Eston和15%(458-57)在低病压。未喷药地块的产量损失为26% ~ 57%。458-57年下降了20 - 28%,其中cv损失最为显著。埃斯顿病压高应用程序……
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引用次数: 32
Herbicide-induced disease resistance and associated increases in free amino acid levels in melon plants 除草剂诱导的甜瓜植株抗病性及其相关的游离氨基酸水平升高
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600129
A. Starratt, G. Lazarovits
Abstract Melon seedlings grown in the presence of low levels of the dinitroaniline herbicide trifluralin and the chloroacetamide herbicide acetochlor to induce resistance to Fusarium oxysporum f. sp. melonis (the cause of fusarium wilt) had elevated levels of free amino acids. This response with trifluralin is similar to that previously described for tomato seedlings treated with various dinitroanilines. In tomato, however, acetochlor did not induce resistance to F. oxysporum f. sp. lycopersici or result in elevated levels of amino acids.
摘要在低剂量二硝基苯胺类除草剂三氟乐林和氯乙酰胺类除草剂乙草胺诱导抗性的条件下,甜瓜幼苗游离氨基酸水平升高。氟乐灵的这种反应与先前描述的用各种二硝基苯胺处理的番茄幼苗的反应相似。然而,在番茄中,乙草胺并没有诱导番茄尖孢菌的抗性,也没有导致氨基酸水平升高。
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引用次数: 18
ACREMONIUM RECIFEI : A NEW CAUSAL AGENT OF MACADAMIA QUICK DECLINE 一种新的澳洲坚果快速衰退的诱因
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600131
W. Ko, R. Kunimoto
Abstract Quick decline is the most serious disease of macadamia trees in Hawaii. Some macadamia trees showing symptoms of quick decline in commercial orchards on the island of Hawaii did not display macroscopic fungal fruiting bodies on the trunks. Acremonium recifei was consistently isolated from bark and wood obtained from trunks of these trees. The fungus was demonstrated to kill twigs of healthy macadamia trees when these twigs were artificially inoculated. Results suggest that trunk infection by A. recifei may lead to girdling, resulting in a rapid decline of macadamia trees.
速降病是夏威夷夏威夷坚果树最严重的病害。在夏威夷岛的商业果园中,一些表现出快速衰退症状的澳洲坚果树在树干上没有显示肉眼可见的真菌子实体。从这些树木的树皮和树干上获得的木材中一直可以分离到栎木。当这些树枝被人工接种时,真菌被证明可以杀死健康的夏威夷树的树枝。结果表明,累日假丝虫侵染澳洲坚果树干后,会造成树干的缠绕,导致果树数量迅速下降。
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引用次数: 3
Density dynamics of Gliocladium roseum in relation to biological control of Botrytis cinerea in red raspberry 红覆盆子中玫瑰胶霉密度动态与灰霉病生物防治的关系
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600117
Hai Yu, J. Sutton
Abstract Density patterns of colony-forming units (CFU) of the biocontrol agent Gliocladium roseum were investigated in raspberry cv. Boyne and cv. Redwing after spore suspensions (107 conidia/mL) were applied 2 h before dusk in field plots. Estimated density of G. roseum on leaves of both cultivars was 2 x 103 to 5 x 103 CFU/cm2 at 1 h after inoculation. During the next 2 days density of the agent progressively decreased, but at 3–10 days after inoculation it fluctuated around 10–102 CFU/cm2 leaf in ‘Boyne’ and after or 3–12 days, it fluctuated near 103 CFU/cm2 leaf in ‘Redwing’. Density of G. roseum on flowers was 1 x 104 to 5 x 104 CFU/flower at 1 h after inoculation and declined rapidly in ‘Boyne’ and slowly in ‘Redwing’. At 3–10 and 3–12 days, respectively, the density fluctuated around 70 CFU/flower in ‘Boyne’ and 800–2300 CFU/flower in ‘Redwing’. Germination of G. roseum, observed on stamens of ‘Boyne’, was sparse (<4–10%) in the field. However, germination potential, estimated in stamens of flower...
摘要研究了覆盆子菌落形成单位(CFU)的密度分布规律。博因和cv。黄昏前2 h在田间地块施用孢子悬浮液(107分生孢子/mL)后的红翅。接种后1 h, 2个品种叶片上的玫瑰菌密度为2 × 103 ~ 5 × 103 CFU/cm2。在接下来的2天内,药剂密度逐渐下降,但在接种后3-10天,' Boyne '的浓度在10-102 CFU/cm2左右波动,在3-12天后,' Redwing '的浓度在103 CFU/cm2附近波动。接种后1 h,花上密度为1 × 104 ~ 5 × 104 CFU/花,‘Boyne’下降快,‘Redwing’下降慢。在3-10天和3-12天,‘Boyne’和‘Redwing’的密度分别在70 CFU/朵和800-2300 CFU/朵左右波动。在‘Boyne’的雄蕊上观察到的蔷薇萌发率很少(< 4-10%)。然而,在花的雄蕊中估计的发芽势…
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引用次数: 18
Mycelial growth and production of oxalic acid by virulent and hypovirulent isolates of Sclerotinia sclerotiorum 菌核菌强毒株和弱毒株菌丝生长和草酸的产生
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600090
T. Zhou, G. Boland
Abstract Hypovirulent dsRNA-containing, and virulent dsRNA-free, isolates of Sclerotinia sclerotiorum were compared for mycelial growth and oxalic acid production in liquid culture. When harvested at 14 days post-inoculation, hypovirulenr isolates 91 and 215 produced less dry mycelium than virulent isolates 191 and 275C in potato-dextrose broth (PDB) and PDB amended with sodium succinate (PDBSS). All isolates produced more oxalic acid per gram dry mycelium When grown in PDBSS than in PDB. In a time course study, hypovirulent isolates 91 and 275 produced less mycelium (0.23 and 0.03 g dry wt.) and oxalic acid (0.22 and 0.01 mg/mL) than virulent isolates 191 and 275C (0.81 and 0.91 g dry wt., and 0.50 and 0.63 mg oxalic acid per millilitre, respectively) at 15 days post-inoculation. The amount of oxalic acid produced per gram dry mycelium reached a maximum for virulent isolates 191 and 275C at 3 and 5 days post-inoculation. Hypovirulent isolate 91 reached a maximum at 9 days post-inoculation, and hypovirule...
摘要本文比较了含低毒力dsrna和不含强毒力dsrna的菌核菌菌丝体在液体培养中的菌丝生长和草酸产量。接种后14天收获时,低毒力菌株91和215在马铃薯葡萄糖肉汤(PDB)和经丁二酸钠(PDBSS)修饰的PDB中产生的干菌丝少于强毒菌株191和275C。所有分离株在PDBSS中比在PDB中产生更多的草酸每克干菌丝体。在一项时间过程研究中,弱毒菌株91和275在接种后15天产生的菌丝体(0.23和0.03 g干重)和草酸(0.22和0.01 mg/mL)少于强毒菌株191和275C(分别为0.81和0.91 g干重,0.50和0.63 mg草酸/毫升)。毒力菌株191和275C在接种后第3天和第5天每克干菌丝体产生的草酸量达到最大值。低毒力分离物91在接种后9天达到最大值。
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引用次数: 53
Evaluation of Colletotrichum sp. and Fusarium spp. as potential biological control agents for marsh reed grass (Calamagrostis canadensis) 炭疽菌和镰刀菌作为沼泽芦苇潜在生物防治剂的评价
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600115
R. Winder
Abstract Marsh reed grass (Calamagrostis canadensis [Michx.] Beauv.), a plant that limits conifer growth in boreal forest regeneration, was sampled in western Canada for the presence of potential fungal biocontrol agents. Of 20 isolates screened for pathogenicity, Colletotrichum sp., Fusarium spp., and Dilophospora alopecuri (Fr.:Fr.) Fr. were pathogenic to marsh reed grass. Colletotrichum isolate PFC13 caused up to 54% damage when formulated in powdered alginate and applied with a vegetable oil and surfactant combination. The virulence of Fusarium isolates varied with isolate and incubation conditions, while the virulence of isolate PFCI3 was sensitive to formulation conditions. Fusarium isolates had a broad host range, causing up to 78% leaf area damage on marsh reed grass seedlings. Isolate PFC13 had a narrower host range than the Fusarium isolates, including oat, sunflower, and rye, but not other plants such as corn or wheat. Fungi have potential as biocontrol agents of marsh reed grass when considere...
沼泽芦苇(Calamagrostis canadensis);[Beauv.]),一种在北方森林再生中限制针叶树生长的植物,在加拿大西部取样,寻找潜在的真菌生物防治剂的存在。致病性筛选的20株分离菌中,炭疽菌、镰刀菌和褐皮枯病菌(Fr.:Fr.)对沼泽芦苇有致病性。当将炭疽菌分离PFC13配制成海藻酸粉并与植物油和表面活性剂混合使用时,其危害可达54%。镰刀菌分离株的毒力随分离条件和培养条件的不同而不同,而PFCI3分离株的毒力对配方条件敏感。分离镰刀菌寄主范围广,可对芦苇幼苗造成78%的叶面积损害。PFC13分离株的寄主范围比镰刀菌分离株窄,包括燕麦、向日葵和黑麦,但不包括玉米或小麦等其他植物。真菌有潜力作为沼泽芦苇的生物防治剂。
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引用次数: 12
Three new rust resistance genes in flax introductions 亚麻引种的三个新的抗锈病基因
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10599970
K. Rashid, E. Kenaschuk
Abstract The genetics of resistance to flax rust (caused by Melampsora lini) in six flax (Linum usitatissimum) introductions from Argentina were investigated in a series of crosses with single-gene lines for rust resistance. These introductions proved resistant to the most virulent rust races in the North American collection of M. lini. Segregating patterns in F2 and BCF1 populations from crosses with the effective rust resistance genes indicated the presence of three resistance genes designated as Al, A2, and A3. These genes are different from the rust resistance genes present in the 30 single-gene lines used to differentiate the North American races of M. lini
摘要对6个阿根廷引种亚麻(Linum usitatissimum)的抗锈病遗传进行了研究。事实证明,这些引种对北美M. lini集合中最毒的锈病种具有抵抗力。有效抗锈基因杂交组合F2和BCF1群体的分离模式表明,存在3个抗锈基因,分别为Al、A2和A3。这些基因不同于30个单基因系中存在的抗锈病基因,这些单基因系被用来区分北美白僵菌种
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引用次数: 1
Genetic variation between strains of Monilinia fructicola and Monilinia laxa isolated from cherries in Michigan 密歇根樱桃蒙利菌与松拉蒙利菌的遗传变异
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10599997
C. Snyder, A. Jones
Abstract Polymerase chain reaction (PCR)-mediated analysis of rDNA from isolates of Monilinia fructicola and Monilinia laxa from Michigan cheny orchards revealed interspecies restriction site variation in the internal transcribed spacer 1 (ITSI) region and length variation in the small subunit (SSU) rRNA gene. 1TS1 sequences from both species were 146 by long; however, the ITSI of M. laxa differed at three positions from the ITSI of M. fructicola. Although the sequences of the ITS1 regions from both species were nearly identical, the enzyme tilrel cuts the PCR-amplified ITSI region of the two species differentially. PCR amplification of the 3′ end of the SSU rRNA gene yielded products of approximately 940 and 520 by from M. fructicola and M. laxa, respectively. A 421-bp group I intron was detected by PCR within the SSU rDNA of 32 isolates of M. fructicola but not in the eight isolates of M. laxa. Intron sequences from each of four isolates of M. fructicola were identical, and the SSU rDNA flanking sequenc...
摘要利用聚合酶链反应(PCR)技术对美国密歇根果园产的果假单胞菌(Monilinia fructicola)和散假单胞菌(Monilinia laxa)分离株的rDNA进行分析,揭示了其内部转录间隔区1 (ITSI)限制性位点的变异和小亚单位(SSU) rRNA基因长度的变异。两种的1TS1序列长度均为146;但在3个位置上,松果霉的ITSI与松果霉的ITSI存在差异。虽然这两个物种的ITS1区域序列几乎相同,但tilrel酶切割pcr扩增的ITSI区域是不同的。对SSU rRNA基因的3′端进行PCR扩增,分别从果霉和laxa中得到约940和520个产物。在32株果实分枝杆菌的SSU rDNA中检测到一个421 bp的I族内含子,而在8株laxa分枝杆菌中未检测到该内含子。四个分离株的内含子序列相同,SSU rDNA侧翼序列相同。
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引用次数: 39
期刊
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie
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