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Stem rusts on wheat, barley, and oat in Canada in 1996 and 1997 1996年和1997年加拿大小麦、大麦和燕麦的茎锈病
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501210
D. Harder
In 1996, stem rusts of wheat and barley [Puccinia graminis f. sp. tritici] and oat [P. graminis f. sp. avenae] were light in eastern Canada, and in western Canada they were confined mainly to the Red River Valley of Manitoba, where maximum severities of 40% occurred in lines of susceptible wheat in nurseries. In 1997 only traces of stem rust were found on any host at any location. There were no infections in commercial wheat fields in 1996 or 1997. Only trace levels of stem rust were found in cultivated barley both years. There was no stem rust in commercial oat fields in 1996 or 1997. Infections in wild oat did not exceed 15% in either year and occurred only in Manitoba. In 1997, most isolates collected from cultivated or wild barley, and about one-half of those collected from wheat, were rye stem rust [P. graminis f. sp. secalis]. Pathotype identification was made on only 14 collections obtained from wheat, and on 19 from wild or cultivated barley in Manitoba in 1997. During both years, 24 pathotypes of...
1996年,小麦和大麦茎锈病[小麦锈病]和燕麦茎锈病[P。黑麦病在加拿大东部发病率较低,在加拿大西部主要局限于马尼托巴省的红河谷,在苗圃的易感小麦品系中发病率最高达40%。1997年,在任何地点的任何寄主身上只发现了茎锈病的痕迹。1996年和1997年商品麦田没有感染。在栽培大麦中只发现了微量的茎锈病。1996、1997年商品燕麦田未发生茎锈病。两年中野生燕麦的感染率均不超过15%,仅发生在马尼托巴省。1997年,从栽培或野生大麦中收集到的大多数分离株,以及从小麦中收集到的大约一半的分离株,都是黑麦茎锈病。禾本科植物[m]。1997年在马尼托巴省仅对14份小麦和19份野生或栽培大麦进行了病原型鉴定。在这两年中,24种病理类型…
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引用次数: 7
Characterization and effectiveness of Phialophora spp. isolated from a Montana take-all suppressive soil in controlling take-all disease of wheat 蒙大拿州全蚀性土壤分离菌的鉴定及其防治小麦全蚀性病害的效果
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501200
N. Zriba, J. Sherwood, D. Mathre
Selected fungi from a take-all suppressive wheat field in Montana were characterized morphologically and identified as Phialophora spp. Ribosomal DNA (rDNA) fragments from four Phialophora spp. and two known Gaeumannomyces isolates were amplified with polymerase chain reaction (PCR) using universal primers, cloned, and sequenced. Sequence comparison of the rDNA ITS regions revealed that these Phialophora isolates were not closely related to Gaeummanomyces sp. Alignment of the ITS sequences allowed the design of PCR primers that distinguished the Phialophora isolates from Gaeummanomyces sp. as well as Phialophora sp. related to Gaeummanomyces. Phialophora sp. 1-52 and Phialophora sp. 1-58 were tested alone and in combination for suppression of Gaeumannomyces graminis var. tritici in the field. When introduced on autoclaved canola seed, 1-52 proved to be an efficient biological control agent against wheat take-all disease in its original suppressive soil, as well as in a highly conducive soil. Isolate 1-58 ...
采用聚合酶链反应(PCR)技术,对4株Phialophora spp和2株Gaeumannomyces分离株的核糖体DNA (rDNA)片段进行扩增、克隆和测序。rDNA ITS区序列比较表明,这些Phialophora分离物与Gaeummanomyces sp.的亲缘关系并不密切。通过对ITS序列的比对,可以设计PCR引物,区分Phialophora分离物与Gaeummanomyces sp.以及与Gaeummanomyces相关的Phialophora sp.。在田间试验中,对Phialophora sp. 1-52和Phialophora sp. 1-58单独和联合施用对小麦芽孢杆菌的抑制作用进行了试验。结果表明,1-52对小麦全蚀病具有良好的生物防治效果。隔离1-58…
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引用次数: 13
Viability of oospores of Phytophthora infestans under field conditions in northeastern North America 北美东北部病原菌卵孢子在田间条件下的生存能力
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501204
M. Medina, H. W. Platt
Studies were conducted in potato fields on Prince Edward Island (PE) and New Brunswick (NB) to determine the overwintering viability, germination, and infectivity of oospores of Phytophthora infestans. Oospore viability was determined using plasmolysis (4 M NaCl) and tetrazolium bromide (MTT) tests. After seven months exposure to winter conditions in potato fields, the viability of oospores buried on PE and NB ranged from 2.5 to 13% and from 1 to 28%, respectively, with the plasmolysis test and from 13 to 44% and from 2.5 to 40%, respectively, with the MTT test. In other studies on PE, oospore viability ranged from 5 to 15% 12 months after burial. In laboratory studies, oospores exposed to temperatures of -20 and -50°C for up to 90 days had viabilities greater than 50%, whereas exposure to 0, 4, or 15°C resulted in average viabilities from 23 to 36%. The lowest viability, 22%, was recorded at the highest temperature tested, 36°C. Oospore germination was low, ranging from 6 to 19%. However, this level was ...
在爱德华王子岛(PE)和新不伦瑞克省(NB)的马铃薯田研究了疫霉(Phytophthora infestans)卵孢子的越冬活力、萌发和感染性。采用质解(4 M NaCl)和溴化四氮唑(MTT)试验测定卵孢子活力。在马铃薯田冬季条件下暴露7个月后,PE和NB上埋埋的卵孢子存活率分别为2.5% ~ 13%和1% ~ 28%,MTT试验分别为13% ~ 44%和2.5% ~ 40%。在其他关于PE的研究中,埋葬后12个月的卵孢子存活率为5%至15%。在实验室研究中,卵孢子暴露于-20和-50°C温度下长达90天的存活率大于50%,而暴露于0、4或15°C的平均存活率为23%至36%。在最高温度36°C时,存活率最低,为22%。卵孢子发芽率低,在6% ~ 19%之间。然而,这个水平是……
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引用次数: 29
Comparison of polymerase chain reaction based methods with plating on media to detect and identify verticillium wilt pathogens of potato 基于聚合酶链反应法与培养基上电镀法检测鉴定马铃薯黄萎病病菌的比较
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501202
G.S. Mahuku, H.W. (Bud) Platt, P. Maxwell
A polymerase chain reaction (PCR) based assay that utilizes specific primers was compared with assays involving plating on selective agar media for its ability to detect and identify verticillium wilt pathogens of potato following artificial inoculations under field conditions. The common Verticillium species were detected in stem tissues and soil using both methods. The PCR assay was faster and efficient, requiring only 2 days for positive species identification, whereas the media-plating method required more than 4 weeks. Verticillium albo-atrum strain 1 (VA1) was the most frequently recovered species in soil and stems that were assayed using PCR and plating methods. However, the weakly pathogenic V. albo-atrum strain 2 (VA2) could not be differentiated from the more aggressive VA1 strain using the plating on media method. Verticillium tricorpus was detected at very low levels in plots that had been inoculated with this species, whereas VA2 was not detected in assayed potato stems using either method. H...
利用特异性引物的聚合酶链反应(PCR)方法与选择性琼脂培养基上的电泳方法比较了其在田间条件下人工接种后检测和鉴定马铃薯黄萎病病菌的能力。两种方法均在茎组织和土壤中检测到常见黄萎病菌。PCR检测速度快、效率高,仅需要2天就能鉴定出阳性菌种,而培养皿法则需要4周以上。采用PCR和平板法检测的黄萎病菌株1 (VA1)是土壤和茎中检出最多的菌种。然而,弱致病性白斑弧菌菌株2 (VA2)不能与更具侵袭性的VA1菌株用介质镀法进行区分。在接种该菌种的样地中检测到极低水平的三体黄萎病菌,而在两种方法的马铃薯茎中均未检测到VA2。H……
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引用次数: 34
The development and implementation of strategies to prevent resistance to B.t.- expressing crops: an industry perspective 发展和实施策略,以防止抗bt表达作物:一个行业的观点
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501198
J. Riebe
Genes for the production of various Bacillus thuringiensis (B.t.) proteins have been incorporated into plants through the techniques of biotechnology. Since 1995, corn, cotton, and potato seed products containing B.t. genes have been introduced to the market. Simultaneous to the development and introduction of B.t. plants, tremendous research efforts were made by industry, academic, and government scientists to devise product-specific insect resistance management (IRM) programs. IRM has become an essential part of the development and regulatory review process for B.t.-expressing plants, as distinct from other conventional chemistries. Despite this unprecedented effort, the long-term success of insect-protected crops or other pest control tools ultimately depends on the transfer of information to, and adoption by, individual growers, The effective implementation of proactive IRM plans will require integration and interaction between regulators, manufacturers of the technology, seed companies, agri-chemical...
通过生物技术已将生产苏云金芽孢杆菌(B.t.)各种蛋白质的基因植入植物中。自1995年以来,含有B.t.基因的玉米、棉花和马铃薯种子产品已被引入市场。在开发和引进B.t.植物的同时,工业界、学术界和政府的科学家们进行了大量的研究工作,以设计针对特定产品的抗虫管理(IRM)计划。与其他传统化学物质不同,IRM已成为表达bt的植物开发和监管审查过程的重要组成部分。尽管付出了前所未有的努力,但防虫作物或其他害虫控制工具的长期成功最终取决于信息的传递和个体种植者的采用。积极主动的IRM计划的有效实施将需要监管机构、技术制造商、种子公司、农化…
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引用次数: 10
Studies on winter survival strategies of Erysiphe pisi in Manitoba 曼尼托巴省褐皮蝇冬季生存策略的研究
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501207
K. R. Tiwari, T. Warkentin, G. Penner, J. Menzies
Erysiphe pisi, the causal agent of powdery mildew, is an important disease of field pea in western Canada, but very little information is available on whether or how it survives the winter in the prairie provinces. Therefore, studies were conducted of the role of cleistothecia on pea stubble, the possibility of seed transmission, and survival on other plant species. In 1996 and 1997 cleistothecia were abundantly formed on heavily infected plants in late August to September under field conditions. Microscopic observation during the winter indicated that by May more than 95% of the cleistothecia had degenerated under field conditions, whereas in samples stored at room temperature, 50% of the cleistothecia contained intact ascospores. When seeds from plants heavily infected with powdery mildew were grown in a greenhouse, none of the 4200 plants examined developed powdery mildew symptoms, suggesting that the possibility of transmission of E. pisi through infected seed is remote. When isolates of powdery milde...
白粉病(Erysiphe pisi)是加拿大西部大田豌豆的一种重要病害,但关于它是否或如何在草原省份过冬的信息很少。因此,我们开展了闭锁藻在豌豆残茬上的作用、种子传播的可能性以及在其他植物上的存活研究。1996年和1997年8月下旬至9月,在田间条件下,在严重侵染的植株上大量形成锁卵绦虫。冬季的显微观察表明,到5月,95%以上的闭锁囊虫在野外条件下已经退化,而在室温下保存的样品中,50%的闭锁囊虫含有完整的子囊孢子。当严重感染白粉病的植物的种子在温室中生长时,4200株被检查的植物中没有一株出现白粉病症状,这表明通过受感染的种子传播pisi的可能性很小。当分离的白粉病…
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引用次数: 6
Hypotheses for the inter-regional movement of new genotypes of Phytophthora infestans in Canada 加拿大疫霉新基因型跨区域传播的假设
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501203
R. Peters, H. W. Platt, R. Hall
During late blight surveys conducted from 1994 to 1996, hypotheses were developed to explain the migration of novel genotypes of Phytophthora infestans between regions or provinces via infected potato and tomato tissues and via winds of tropical storm systems. The appearance of isolates of the US-7 genotype in Alberta (a province affected predominantly by US-l) in 1994, was believed to be due to infected seed tubers from British Columbia. Similarly, the initial isolation of the US-8 genotype in Nova Scotia in 1995 and in Newfoundland in 1996 could be explained by the importation of infected potato seed tubers from other regions. The discovery of Al, metalaxyl-insensitive (glucose phosphate isomerase banding pattern = 100/]00/111; gll or US-ll) isolates of P. infestans in a tomato field in Ontario in 1996 (a province affected predominantly by US-8), suggested the introduction of this new strain had occurred via infected or infested tomato tissue. Circumstantial evidence was also obtained for the introducti...
在1994年至1996年进行的晚疫病调查中,提出了一些假设,以解释新基因型的疫霉菌通过受感染的马铃薯和番茄组织以及热带风暴系统的风在地区或省份之间的迁移。1994年在阿尔伯塔省(一个主要受us - 1影响的省份)出现US-7基因型分离株,据信是由于来自不列颠哥伦比亚省的感染种块茎。同样,1995年新斯科舍省和1996年纽芬兰最初分离出US-8基因型可能是由于从其他地区进口了受感染的马铃薯种块茎。发现Al,甲酰基不敏感(葡萄糖磷酸异构酶带型= 100/]00/111;1996年在安大略省(一个主要受US-8影响的省份)的一个番茄地里发现了gll或US-ll)分离株,表明这种新菌株是通过受感染或侵染的番茄组织引入的。还获得了引证的间接证据。
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引用次数: 8
REDUCTION OF ROOT-LESION NEMATODES (PRATYLENCHUS PENETRANS) IN TUBERS OF POTATO (SOLANUM TUBEROSUM) DURING COLD STORAGE 马铃薯块茎冷藏过程中根损线虫的减少
Pub Date : 1999-06-01 DOI: 10.1080/07060669909501206
T. Olthof, Qing Yu
The effect of storage duration at low temperatures on numbers of the nematode Pratylenchus penetrans harbored within potato tubers of cvs. Superior, Monona, Saginaw, Saginaw Gold, Yukon Gold, and Kennebec were studied from 1992 to 1995. Numbers of P. penetrans in potato tubers held at 1-5?°C for 8 months decreased to nearly zero, regardless of cultivar. The number of nematodes decreased at different rates when stored at 3-5°C than when stored at 1-3°C and decreased most rapidly during the first 2 months of storage at 1-3°C; at 3-5°C storage, it took 4 months for the numbers to decrease substantially. The number of live nematodes in potato tubers after 8 months of storage below 5°C was too low to pose a significant threat at planting to already infested fields, but would still be a source of a new infestation.
低温贮藏时间对马铃薯块茎内渗透密叶线虫数量的影响。1992年至1995年对苏必利尔河、莫诺纳河、萨吉诺河、萨吉诺黄金河、育空黄金河和肯纳贝克河进行了研究。马铃薯块茎中穿透性假单胞菌的数量控制在1 ~ 5?8个月的温度下降到几乎为零,与品种无关。3-5℃贮藏与1-3℃贮藏时线虫数量减少的速率不同,1-3℃贮藏前2个月减少最快;在3-5℃的贮藏条件下,4个月后数量大幅减少。在低于5°C的条件下储存8个月后,马铃薯块茎中活线虫的数量过低,不足以对已经侵染的田地的种植构成重大威胁,但仍将成为新的侵染的来源。
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引用次数: 5
Genotypic and pathogenic variation of Colletotrichum gloeosporioides f.sp. malvae. 炭疽菌的基因型及致病变异。malvae。
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10600130
H. R. Kutcher, K. Mortensen
Abstract Colletotrichum gloeosporioides f. sp. malvae (C.g. malvae) is pathogenic on velvetleaf (Abutilon theophrasti) but does not cause sufficient damage or mortality to be considered as a biological control agent for this weed. Velvetleaf control might be increased if isolates of C.g. malvae with greater pathogenicity could be identified. C.g. malvae isolates from Saskatchewan, Manitoba, and Ontario (Canada) were examined to determine their genotypic and pathogenic variation. The random amplified polymorphic DNA (RAPD) technique was used to determine differences among isolates based on the presence or absence of amplified DNA fragments. Isolates were also tested for variation in pathogenicity by stem injection of the pathogen into velvetleaf plants under controlled conditions. All isolates of C.g. malvae were highly pathogenic on velvetleaf. Differences in RAPD patterns were not obtained among C.g. malvae isolates. However, differences in RAPD patterns could be used to differentiate isolates of C.g. ma...
摘要/ Abstract摘要:炭疽病菌(Colletotrichum gloeosporioides f. sp. malvae, C.g. malvae)对棉叶(Abutilon theophrasti)具有致病性,但不会造成足够的危害或死亡,因此不能被认为是一种生物防治剂。如果能鉴定出致病性较强的malvae分离株,可能会增加对天鹅绒叶的控制。对来自萨斯喀彻温省、马尼托巴省和加拿大安大略省的C.g. malvae分离株进行了检测,以确定其基因型和致病变异。采用随机扩增多态性DNA (RAPD)技术,根据扩增DNA片段的存在与否来确定菌株之间的差异。在控制条件下,将病原菌茎注射到丝绒叶植物中,检测分离株的致病性变化。所有分离株均对丝绒叶具有高致病性。不同分离株间的RAPD模式没有差异。然而,RAPD模式的差异可以用来区分菌株。
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引用次数: 2
Field and growth chamber evaluation of components of partial resistance to Colletotrichum truncatum in lentil 扁豆部分抗炭疽病成分的田间和生长室评价
Pub Date : 1999-03-01 DOI: 10.1080/07060661.1999.10599969
G. Chongo, C. C. Bernier
Abstract The components of panial resistance to anthracnose (Colletotrichum truncatum) were evaluated in lentil (Lens culinaris) in a growth chamber and in the field. Lines PI 299331, PI 320937, PI 345629, 458-57, and cv. Indianhead, with different levels of panial resistance and the susceptible cultivar Eston were inoculated in a growth chamber and in the field using a single virulent isolate of C truncatum. Incubation and latent periods as well as sporulating lesions were measured on stems. A computer imaging system was used to measure lesion number, lesion size, and percent necrotic area per square centimetre on stem samples. Area under the disease progress curve (AUDPC) values were also calculated using disease severity from the field samples. Lines PI 320937, PI 345629, 458-57, and cv. Indianhead were found to have high levels of partial resistance, as indicated by significantly smaller and fewer lesions and longer incubation and latent periods than cv. Eston. For each component, PI 299331 was interm...
摘要在生长室内和田间对扁豆(Lens culinaris)抗炭疽病(Colletotrichum truncatum)的成分进行了评价。行PI 299331, PI 320937, PI 345629, 458-57,和cv。用一株强毒分离株在室内和田间分别接种不同抗蚜水平的印度头菌和敏感品种Eston。在茎上测量了潜伏期和潜伏期以及孢子病变。计算机成像系统用于测量病变数量、病变大小和茎样每平方厘米坏死面积百分比。利用田间样品的疾病严重程度计算疾病进展曲线下面积(AUDPC)值。行PI 320937, PI 345629, 458-57,和cv。发现印度头虱具有高水平的部分抗性,这表明病变明显更小,更少,潜伏期和潜伏期比cv更长。Eston。对于每个部件,PI 299331是临时的…
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引用次数: 32
期刊
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie
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