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Biological control of postharvest diseases of peach with phyllosphere isolates of Pseudomonas syringae 丁香假单胞菌层球分离菌对桃采后病害的生物防治
Pub Date : 1999-12-01 DOI: 10.1080/07060669909501174
T. Zhou, J. Northover, K. Schneider
Commercially ripe peaches were wounded and co-inoculated with phylloplane isolates of Pseudomonas syringae (MA-4 and NSA-6), Pseudomonas fluorescens (BAP-3), or an isolate of Candida sp. (NSD-4) in combination with either conidia of Monilinia fructicola (cause of brown rot) or sporangiospores of Rhizopus stolonifer (cause of rhizopus rot). Isolates NSA-6 and MA-4 at a concentration of 1 x 107 CFU/mL reduced brown rot to 28% and 73%, respectively, from 98% in the inoculated check after 5 days incubation at 22°C. Both isolates reduced rhizopus rot to 5% and 8% from 53% in the inoculated check after 5 days incubation. The P. syringae isolates when used in 2-min soak treatments were less effective against natural, preharvest M. fructicola infections of late-harvested peaches. Isolates MA-4 and NSA-6 suppressed brown rot from 63% to 30% and from 95% to 71-81%, respectively, after 3 and 4 days incubation at 22°C. The use of 0.5% CaCl2 in the soak suspension significantly improved the activity of P. syringae but...
用丁香假单胞菌(MA-4和NSA-6)、荧光假单胞菌(bape -3)或念珠菌(NSD-4)的叶状面分离物与引起褐腐病的果实念珠菌(Monilinia fructicola)或匍匐茎根霉(Rhizopus stolonifer)的分生孢子(sporangio孢子)共同接种成熟的桃子。在22℃培养5天后,浓度为1 × 107 CFU/mL的NSA-6和MA-4分离株的褐腐病率分别从98%降低到28%和73%。经过5天的培养,这两种分离株都将根霉腐病率从接种检查的53%降低到5%和8%。丁香假单胞菌分离株在2分钟浸泡处理时,对收获晚的桃子的自然、收获前的果假单胞菌感染效果较差。分离菌株MA-4和NSA-6在22°C条件下培养3天和4天后,对褐腐病的抑制作用分别为63% ~ 30%和95% ~ 71 ~ 81%。添加0.5% CaCl2的浸泡悬浮液显著提高了丁香假单胞菌的活性。
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引用次数: 53
Genetic diversity among Fusarium graminearum strains from Ontario and Quebec 安大略省和魁北克省禾谷镰刀菌的遗传多样性
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501196
M. Dusabenyagasani, D. Dostaler, R. Hamelin
The random amplified polymorphic DNA (RAPD) method was used to sample DNA markers from 72 strains of Fusarium graminearum isolated from wheat blighted kernels collected from Quebec (52 strains), Ontario (18 strains), and Prince Edward Island (2 strains). Sixty-five markers generated with eight RAPD primers showed that all strains were genetically distinct. Moreover, the analysis of molecular variance (AMOVA) indicated that 90.56% of the genetic variability associated with strains used in this study was explained by within-region variation. The GST values were low and showed that there was almost no genetic differentiation associated with sampling F. graminearum either from two different years in Quebec (GST = 0.0375) or from Ontario and Quebec (GST = 0.0509). These results show that F. graminearum strains from Quebec and Ontario might be part of a single population pool.
采用随机扩增多态性DNA (RAPD)方法,对从加拿大魁北克(52株)、安大略省(18株)和爱德华王子岛(2株)小麦枯萎病籽粒中分离的72株稻瘟病镰刀菌进行了DNA标记分析。用8个RAPD引物生成的65个标记表明,所有菌株在遗传上是不同的。分子变异分析(AMOVA)表明,90.56%的遗传变异可解释为区域内变异。GST值较低,表明在魁北克(GST = 0.0375)和安大略和魁北克(GST = 0.0509)两个不同年份取样的禾草赤霉病菌几乎没有遗传分化。这些结果表明,来自魁北克和安大略的谷物F. graminearum菌株可能是单一种群池的一部分。
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引用次数: 52
MOLECULAR STRATEGIES FOR ENGINEERING RESISTANCE TO POTATO VIRUSES 马铃薯病毒工程抗性的分子策略
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501186
L. Kawchuk, D. Prüfer
An increasing number of molecular strategies are available for engineering virus resistance in potatoes (Solatium tuberosum ssp. tuberosum L.) using pathogen-derived or other virus-inhibiting nucleic acid sequences and proteins. Virus resistance has been developed in transgenic plants expressing virus coat proteins, movement proteins, replicases, untranslatable sense or antisense RNAs, proteases, defective interfering RNAs, and satellites. Expression of ribozymes, a double-stranded RNA-specific ribonuclease, antiviral proteins, a plant pathogen resistance gene, and plantibodies has also provided virus resistance. Transgenic plants resistant to several viruses have already been approved by regulatory agencies for commercial release and virus-resistant potato varieties promise to improve yield and quality. Plant protection ranges from being specific against closely related strains of the virus to broad-spectrum resistance that extends to other virus groups. Progress has also been made in combining several s...
越来越多的分子策略可用于马铃薯(Solatium tuberosum ssp)的病毒抗性工程。结核菌(tuberosum L.)利用病原体衍生的或其他抑制病毒的核酸序列和蛋白质。在表达病毒外壳蛋白、运动蛋白、复制酶、不可翻译的正义或反义rna、蛋白酶、有缺陷的干扰rna和卫星蛋白的转基因植物中,已经发展出病毒抗性。核酶、双链rna特异性核糖核酸酶、抗病毒蛋白、植物病原体抗性基因和植物抗体的表达也提供了病毒抗性。对几种病毒具有抗性的转基因植物已经获得监管机构的商业释放批准,而抗病马铃薯品种有望提高产量和质量。植物保护的范围从对密切相关的病毒株的特异性到扩展到对其他病毒群的广谱抗性。在结合几个方面也取得了进展。
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引用次数: 12
CHV3-type dsRNAs and the GH2 genotype in a population of Cryphonectria parasitica in Ontario 安大略寄生蜂种群chv3型dsrna和GH2基因型的研究
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501187
M. S. Melzer, G. Boland
CCP19, a hypovirulent isolate of Cryphonectria parasitica collected from a site near Bothwell, Ontario, in 1987, has shown potential for use in biological control of chestnut blight. CCP19 contains dsRNA that hybridizes with hypovirus CHV3 from isolate GH2 (CHV3-GH2), a hypovirulent isolate of C. parasitica from Michigan. In 1995, 26 isolates of C. parastica were collected at the Bothwell site, and 22-23 (85-89%) of these 26 isolates had significantly smaller lesion diameters in an apple virulence assay than virulent control isolate EP155. Twenty-two of the 26 isolates (85%) contained dsRNA. Segments of dsRNA in all 22 isolates hybridized with cDNA prepared from CHV3-type dsRNA in CCP19. The CHV3-type dsRNA was present in all three MCGs identified in the population. DNA fingerprint analysis revealed a highly clonal population, Twenty isolates had DNA fingerprint genotypes the same as, or similar to, GH2 and 6 isolates had genotypes similar to CCP19. Similarities, including presence of CHV3-type dsRNA, hig...
CCP19是1987年从安大略省Bothwell附近采集的一种低毒分离物,在板栗疫病的生物防治中具有潜在的应用价值。CCP19含有dsRNA,可与来自密歇根州的寄生弧菌低毒分离物GH2 (CHV3-GH2)的低毒分离物CHV3杂交。1995年,在Bothwell地区收集了26株寄生弓形虫,其中22 ~ 23株(85 ~ 89%)的苹果毒力比毒力对照株EP155的病斑直径小。26株分离株中有22株(85%)含有dsRNA。所有22株分离株的dsRNA片段均与CCP19中chv3型dsRNA制备的cDNA杂交。chv3型dsRNA存在于人群中发现的所有三种mcg中。DNA指纹图谱分析显示,该菌株为高克隆群体,与GH2基因型相同或相似的菌株有20株,与CCP19基因型相似的菌株有6株。相似之处包括chv3型dsRNA的存在,高…
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引用次数: 12
Rhizobacterial growth and yield promotion of cucumber plants inoculated with Pythium aphanidermatum 接种疣皮霉对黄瓜根瘤菌生长及产量的促进作用
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501189
R. Utkhede, C. Koch
The potential of rhizobacterial strains to reduce the adverse effects of Pythium aphanidermatum on plant growth and yield was examined under in vitro and greenhouse cropping conditions. Rhizobacterial cultures of 10 strains of Pseudomonas putida, 24 strains of Bacillus subtilis, 4 strains of Enterobacter aerogenes, 1 strain of Bacillus cereus, and 1 unknown bacterial strain were evaluated for antagonism against P. aphanidermatum in laboratory and greenhouse conditions. In a dual culture test on potato dextrose agar, only AG-1 of B. subtilis and strains 2xyta75 and B8 of E. aerogenes were antagonistic to P. aphanidermatum. Under sterile in vitro conditions in pouches, 40 bacterial strains were tested for growth promotion of cucumber (Cucumis sativus) seedlings in the absence of P. aphanidermatum. All bacteria except strain PPG-7-1 of P. putida, strains AGS-3 and AGS-1 of B. subtilis, and strain AG-0 of an unidentified bacterium increased root length of cucumber compared with the P. aphanidermatum inoculate...
在离体栽培和温室栽培条件下,研究了根瘤菌菌株对植物生长和产量的影响。在实验室和温室条件下,对10株恶臭假单胞菌、24株枯草芽孢杆菌、4株产气肠杆菌、1株蜡样芽孢杆菌和1株未知菌株的根细菌培养物进行了拮抗效果评价。在马铃薯葡萄糖琼脂双培养试验中,枯草芽孢杆菌的AG-1和产气芽孢杆菌的2xyta75和B8菌株对隐皮芽孢杆菌具有拮抗作用。在试管无菌条件下,研究了40株细菌在无假单胞菌的情况下对黄瓜幼苗生长的促进作用。除腐臭假单胞菌PPG-7-1、枯草芽孢杆菌AGS-3和AGS-1以及一种未知细菌AG-0外,其余细菌均比接种过的假单胞菌根长增加。
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引用次数: 49
Variation in culture and rDNA among isolates of Sphaeropsis sapinea from Ontario and Manitoba 安大略省和马尼托巴省Sphaeropsis sapinea分离株培养和rDNA的差异
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501188
G. Hausner, J. Reid, A. Hopkin, C. Davis
Three morphotypes were recognized among Manitoba and Ontario isolates of Sphaeropsis sapinea. Variation in culture was assessed based on overall culture morphology, growth rates, and conidial characteristics. Morphotypes A and B conform to previously described morphotypes, but an intermediate morphotype with smooth conidia was designated as morphotype I. Morphotype A isolates were collected from a number of Pinus species as well as Picea pungens. Morphotype B was only recovered from Picea mariana on stressed sites in Ontario, and Pinus banksiana and Pinus resinosa in Manitoba. Morphotype I was recovered from Pinus species and Picea glauca. Restriction fragment length polymorphism analysis of the ribosomal repeat unit grouped the isolates into three classes, or ribotypes, which appear to correspond to the A, B, and 1 morphotypes. All three morphotypes have been collected in Ontario. Restriction fragment length polymorphism analysis of the rDNA repeat unit may therefore be useful in distinguishing Lhe diffe...
在马尼托巴省和安大略省分离的Sphaeropsis sapinea中发现了三种形态。根据总体培养形态、生长率和分生孢子特征来评估培养的变化。形态型A和B符合先前描述的形态型,但具有光滑分生孢子的中间形态型被指定为形态型i。形态型A分离物收集自许多松种和云杉。B型仅在加拿大安大略省的热带云杉(Picea mariana)和马尼托巴省的松(Pinus banksiana)和松林松(Pinus resinosa)中发现。形态型1从松种和云杉种中恢复。核糖体重复单元限制性内切片段长度多态性分析将分离物分为3类或核型,似乎对应于A, B和1形态型。所有三种形态都是在安大略省收集的。因此,rDNA重复单元的限制性内切片段长度多态性分析可能有助于区分不同的dna。
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引用次数: 28
Determining quantitative resistance of barley cultivars at the seedling stage to net blotch caused by Pyrenophora teres 大麦品种苗期对白蛉网斑病抗性的定量测定
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501192
K. Xi, J. Helm, P. A. Burnett, T. Turkington, A. Tekauz
Four barley (Hordeum vulgare L.) cultivars, Heartland, Harrington, Johnston, and Kasota, were compared a: the seedling stage for reactions to the net and spot forms of net blotch caused by Pyrenophora teres f. teres and P. teres f. maculata, respectively. Effects of leaf wetness period, leaf position, and inoculum concentration on lesion development were investigated. The linear relationship between inoculum concentration and diseased leaf area was used to estimate inoculum concentrations causing 50% diseased leaf area (ED50) on leaf 2 (the second seedling leaf) and causing 15% diseased leaf area (ED15) on leaf 3 (third seedling leaf). Resistance of cultivars was compared on the basis of ED50 and ED15. The linear relationship of diseased leaf area with time was used to estimate the rate of lesion spread and to compare resistance. Lack of a significant linear relationship over time on leaf 3 for cvs. Heartland and Kasota inoculated with P. teres f. teres reflected resistance in these two cultivars. Both pa...
比较了四种大麦(Hordeum vulgare L.)品种哈特兰(Heartland)、哈林顿(Harrington)、庄士敦(Johnston)和卡索塔(Kasota)在苗期对白斑致病菌(pyrenophare teres f. teres)和斑点致病菌(P. teres f. maculata)的网纹病和斑点致病菌的反应。研究了叶片湿润期、叶片位置和接种量对病害发展的影响。利用接种量与病叶面积之间的线性关系,估计接种量对2号叶(第2苗叶)和3号叶(第3苗叶)造成的病叶面积(ED15)分别为50%和15%。在ED50和ED15的基础上比较了不同品种的抗性。利用病叶面积随时间的线性关系估计病害扩散速度和比较抗性。叶片3上的cvs随时间缺乏显著的线性关系。在心脏地区和卡索塔地区接种了红圆霉后,这两个品种均表现出抗性。两个爸爸…
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引用次数: 3
Influence of temperature, cultivar, and time on sporulation of Mycosphaerella fragariae on detached strawberry leaves 温度、品种和时间对草莓离体叶片芽孢杆菌产孢的影响
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501191
O. Carisse, B. Peyrachon
The influence of temperature (5-35°C), cultivar (Chambly, Kent, Oka, Tribute) and temperature, and time (0-108 h) or> sporulation of Mycosphaerella fragariae was studied using a detached leaf assay. Sporulation was estimated as the number of conidia produced per lesion and transformed to relative sporulation. For all cultivars, except Oka, for which temperature was not significant, the highest sporulation occurred at 20°C and no sporulation was observed at 35°C. Relative sporulation varied with cultivar, Tribute being the most susceptible, followed by Kent and by Chambly with only few conidia per lesion. Sporulation started at 5°C, increased with increasing temperature up to 20°C, and then decreased. Relative sporulation was modeled using a second-order polynomial of temperature, and the models explained 85.7, 76.4, and 82.2% of the variation for cvs. Chambly, Kent, and Tribute, respectively. On cv. Tribute, less than 5% sporulation was obtained when leaves were exposed to wetness for 24 h or less. At tem...
采用离体叶法研究了温度(5 ~ 35℃)、栽培品种(Chambly、Kent、Oka、Tribute)、温度和时间(0 ~ 108 h)对fragarimycosphaerella产孢量的影响。孢子量估计为每个病变产生的分生孢子数,并转化为相对孢子量。除Oka温度不显著外,所有品种的产孢量在20°C时最高,在35°C时没有产孢。相对产孢量因品种而异,贡品最敏感,肯特次之,尚布莱次之,每个病斑的分生孢子数很少。孢子萌发始于5℃,随着温度的升高而增加,至20℃后逐渐减少。利用温度的二阶多项式对相对产孢量进行建模,模型解释了85.7、76.4和82.2%的相对产孢量变异。分别是Chambly, Kent和Tribute。在简历上。叶片受潮时间不超过24 h,产孢率不到5%。在tem…
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引用次数: 7
RESISTANCE TO POWDERY MILDEW IN GRAPEVINE INDUCED BY PLASMOPARA VITICOLA 葡萄浆原体诱导葡萄对白粉病的抗性
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501190
M. Reuveni
Prior inoculation of grapevine plants with the downy mildew fungus, Plasmopara viticola, induced a resistance to the powdery mildew fungus, Uncinula necator. Fourteen days after exposure to airborne conidia of U. necator, 1.5 and 23.9 colonies of powdery mildew per leaf developed on Plasmopara-inoculated and on control (noninoculated) plants, respectively. This resistance was confined to leaf tissues colonized by P. viticola and was not transferred to the new developing leaves. No protection was achieved when P. viticola failed to infect the plants. Microscopic examination indicated that conidia of U. necator could germinate on leaves infected with downy mildew, but could not produce secondary hyphae and colonies. This protection was reversed by the exogenous foliar application of 0.1% sucrose solution to leaves infected by P. viticola, which resulted in the restoration of susceptibility to powdery mildew. Application of a 0.1 % solution of sucrose enhanced the development of U. necator on both water-trea...
事先给葡萄植株接种霜霉菌(Plasmopara viticola),诱导其对白粉病菌(Uncinula necator)产生抗性。暴露于空气传播的白粉病孢子14 d后,接种浆原菌的植株和未接种浆原菌的对照植株的每叶白粉病菌落分别为1.5个和23.9个。这种抗性仅局限于葡萄霉定殖的叶片组织中,而不会转移到新生的叶片中。当葡萄假单胞菌无法侵染植物时,没有保护作用。显微镜检查表明,乳霜霉病侵染的叶片上孢子能萌发,但不能产生次生菌丝和菌落。在被白粉病感染的叶片上施用0.1%蔗糖溶液后,这种保护作用被逆转,导致白粉病敏感性恢复。应用0.1%的蔗糖溶液,可促进u - necator在水处理和水处理上的发育。
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引用次数: 3
Managing fungal diseases of potato 马铃薯真菌病害的防治
Pub Date : 1999-09-01 DOI: 10.1080/07060669909501184
G. Secor, N. Gudmestad
Potato, Solatium tuberosum, is the fourth-ranked food crop used to support a growing world population because of cultivar versatility and high complex carbohydrate content. Potatoes can be stored for long periods for table and processing markets, but are plagued by storage disease problems. Diseases both in the field and during storage can be limiting factors in sustainable and profitable potato production wherever they are grown. In North America, many diseases caused by fungi are important and require a variety of management practices to reduce them to tolerable economic levels. Such diseases include late blight [Phytophthora infestans], silver scurf [Helminthosporium solani], pink rot [Phytophthora erythroseptica], dry rot [Fusarium sambucinum], verticillium wilt [Verticillium dahliae and Verticillium albo-atrum], black scurf [Rhizoctonia solani], and early blight [Alternaria solani]. These diseases have both a field and storage component, and disease management inputs may be necessary throughout the s...
马铃薯(Solatium tuberosum)是用于支持不断增长的世界人口的第四大粮食作物,因为其品种多功能性和高复合碳水化合物含量。土豆可以长期储存,以供食用和加工市场,但却受到储存疾病的困扰。无论在何处种植马铃薯,田间和贮藏期间的病害都可能成为马铃薯可持续和盈利生产的限制因素。在北美,真菌引起的许多疾病都很重要,需要采取各种管理措施将其减少到可承受的经济水平。这类疾病包括晚疫病(疫霉)、银屑病(茄疫病)、粉红腐病(红败霉)、干腐病(镰刀菌)、黄萎病(大丽黄萎病和白斑黄萎病)、黑腐病(茄根丝核菌)和早疫病(茄交疫病)。这些疾病有田间和储存的组成部分,在整个50年可能需要疾病管理投入。
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引用次数: 156
期刊
Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie
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