Pub Date : 1999-03-01DOI: 10.1080/07060661.1999.10600076
J. Mcdonald, E. Wong, G. Kristjansson, G. P. White
Abstract A simple technique of conducting polymerase chain reaction (PCR) on single ungerminated teliospores of Telleria species was developed. Teliospores were manually cracked under a stereo microscope prior to adding to the PCR reaction mixture. Amplification product was obtained using primers for either a portion of the nuclear ribosomal intergenic spacer region or a portion of the mitochondria) DNA. Collections of teliospores from Tilletia indica, Tilletia barclayana, Tilletia controversa, Tilletia tritici, Tilletia laevis and an unidentified Tilletia sp, from Lolium varied in the proportion of spores from which amplification product could be detected, with the success rate ranging from 100 to 10%. This technique avoids the difficulty and time delay in having to germinate teliospores prior to extracting DNA from a mycelial matte and thus will be of great value in the application of PCR methods for regulatory testing and phylogenetic studies of Tilletia species.
{"title":"Direct amplification by PCR of DNA from ungerminated teliospores of Tilletia species","authors":"J. Mcdonald, E. Wong, G. Kristjansson, G. P. White","doi":"10.1080/07060661.1999.10600076","DOIUrl":"https://doi.org/10.1080/07060661.1999.10600076","url":null,"abstract":"Abstract A simple technique of conducting polymerase chain reaction (PCR) on single ungerminated teliospores of Telleria species was developed. Teliospores were manually cracked under a stereo microscope prior to adding to the PCR reaction mixture. Amplification product was obtained using primers for either a portion of the nuclear ribosomal intergenic spacer region or a portion of the mitochondria) DNA. Collections of teliospores from Tilletia indica, Tilletia barclayana, Tilletia controversa, Tilletia tritici, Tilletia laevis and an unidentified Tilletia sp, from Lolium varied in the proportion of spores from which amplification product could be detected, with the success rate ranging from 100 to 10%. This technique avoids the difficulty and time delay in having to germinate teliospores prior to extracting DNA from a mycelial matte and thus will be of great value in the application of PCR methods for regulatory testing and phylogenetic studies of Tilletia species.","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"73 1","pages":"78-80"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72947867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-03-01DOI: 10.1080/07060661.1999.10600089
K. Conn, G. Lazarovits
Abstract This study examined the effect of chicken, liquid swine, and solid cattle manures on verticillium wilt and potato scab incidence at two commercial farms near Alliston, Ontario. The manures (chicken, 66 t/ha; swine, 55 hLJha; cattle, 100 t/ha) were spread in the spring of 1996 and incorporated with a Rototiller to a depth of 15 cm. Survival of Verticillium dahliae microsclerotia (MS) was determined by burying nylon mesh bags containing MS in the plots. After 4 weeks, the MS were plated on agaz medium. Soil samples collected after incorporation of manures were brought back to the laboratory and used for concurrent laboratory experiments. Ten-gram amounts of soil were placed into test tubes, bags of MS buried in the soil,and survival measured under controlled conditions. Potatoes were planted in the field 1 month after amendment. Potatoes were planted in 1997 and 1998 without any further treatment. verticillium wilt incidence was determined by isolation of V.dahliae from petioles. Scab incidence was...
{"title":"Impact of animal manures on verticillium wilt, potato scab, and soil microbial populations","authors":"K. Conn, G. Lazarovits","doi":"10.1080/07060661.1999.10600089","DOIUrl":"https://doi.org/10.1080/07060661.1999.10600089","url":null,"abstract":"Abstract This study examined the effect of chicken, liquid swine, and solid cattle manures on verticillium wilt and potato scab incidence at two commercial farms near Alliston, Ontario. The manures (chicken, 66 t/ha; swine, 55 hLJha; cattle, 100 t/ha) were spread in the spring of 1996 and incorporated with a Rototiller to a depth of 15 cm. Survival of Verticillium dahliae microsclerotia (MS) was determined by burying nylon mesh bags containing MS in the plots. After 4 weeks, the MS were plated on agaz medium. Soil samples collected after incorporation of manures were brought back to the laboratory and used for concurrent laboratory experiments. Ten-gram amounts of soil were placed into test tubes, bags of MS buried in the soil,and survival measured under controlled conditions. Potatoes were planted in the field 1 month after amendment. Potatoes were planted in 1997 and 1998 without any further treatment. verticillium wilt incidence was determined by isolation of V.dahliae from petioles. Scab incidence was...","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"10 1","pages":"81-92"},"PeriodicalIF":0.0,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86650664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500392
R. Peters, H. W. Platt, R. Hall
Isolates of Phytophthora infestans were obtained from individual fields of potato and tomato and from potato storages across Canada in 1994 (142 samples yielding 555 isolates), 1995 (185 samples yielding 914 isolates), and 1996 (300 samples yielding 1013 isolates). Characterization of these isolates according to mating type and sensitivity to metalaxyl revealed the changing nature of P. infestans populations in Canada. In 1994, isolates of the traditional Al, metalaxyl-sensitive (MS) phenotype were common, but by 1996, they were no longer recovered from any tissue samples. Isolates of the A2 mating type, which were predominantly insensitive to metalaxyl (MI), were prevalent in 1996, except in British Columbia (B.C.). In B.C., Al isolates highly insensitive to metalaxyl predominated. Isolates of the Al, MI phenotype were also found in a sample of tomatoes from Ontario in 1996. New populations composed of the A2 mating type were predominantly insensitive to metalaxyl, but they showed an intermediate respons...
{"title":"CHARACTERIZATION OF CHANGES IN POPULATIONS OF PHYTOPHTHORA INFESTANS IN CANADA USING MATING TYPE AND METALAXYL SENSITIVITY MARKERS","authors":"R. Peters, H. W. Platt, R. Hall","doi":"10.1080/07060669809500392","DOIUrl":"https://doi.org/10.1080/07060669809500392","url":null,"abstract":"Isolates of Phytophthora infestans were obtained from individual fields of potato and tomato and from potato storages across Canada in 1994 (142 samples yielding 555 isolates), 1995 (185 samples yielding 914 isolates), and 1996 (300 samples yielding 1013 isolates). Characterization of these isolates according to mating type and sensitivity to metalaxyl revealed the changing nature of P. infestans populations in Canada. In 1994, isolates of the traditional Al, metalaxyl-sensitive (MS) phenotype were common, but by 1996, they were no longer recovered from any tissue samples. Isolates of the A2 mating type, which were predominantly insensitive to metalaxyl (MI), were prevalent in 1996, except in British Columbia (B.C.). In B.C., Al isolates highly insensitive to metalaxyl predominated. Isolates of the Al, MI phenotype were also found in a sample of tomatoes from Ontario in 1996. New populations composed of the A2 mating type were predominantly insensitive to metalaxyl, but they showed an intermediate respons...","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"28 1","pages":"259-273"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82849687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500387
N. Cerovska, M. Filigarová, T. Moravec, Z. Šubr
Six mouse monoclonal antibodies (MAbs) against potato virus A (PVA) were examined on their reactivity with PVA and its denatured capsid protein (PVA-CP) bound to nitrocellulose membrane. Five MAbs reacted with native PVA, three of them also with PVA-CP. MAb 534 gave no reaction in dot-blot tests. In Western blot analysis only MAbs 151, 290, and 328 reacted with PVA-CP. Competition binding test data confirm mutual spatial proximity of epitopes corresponding to these MAbs. Pepscan (SPOTs tests) with overlapping octapeptides representing the sequence of the first 60 amino acids from the N terminus of PVA-CP showed that the epitopes detected by MAb 151, 328, and 634 are located in this region. MAb 534 was deduced to react with discontinuous CP epitope. Results of analogic peptide synthesis for the MAb 151 epitope indicate that two lysine residues are essential for binding of this MAb to its epitope. Our polyclonal antibodies against PVA reacted with six different regions in this part of the CP.
{"title":"Mapping antigenic epitopes of potato virus A using monoclonal antibodies and overlapping synthetic peptides","authors":"N. Cerovska, M. Filigarová, T. Moravec, Z. Šubr","doi":"10.1080/07060669809500387","DOIUrl":"https://doi.org/10.1080/07060669809500387","url":null,"abstract":"Six mouse monoclonal antibodies (MAbs) against potato virus A (PVA) were examined on their reactivity with PVA and its denatured capsid protein (PVA-CP) bound to nitrocellulose membrane. Five MAbs reacted with native PVA, three of them also with PVA-CP. MAb 534 gave no reaction in dot-blot tests. In Western blot analysis only MAbs 151, 290, and 328 reacted with PVA-CP. Competition binding test data confirm mutual spatial proximity of epitopes corresponding to these MAbs. Pepscan (SPOTs tests) with overlapping octapeptides representing the sequence of the first 60 amino acids from the N terminus of PVA-CP showed that the epitopes detected by MAb 151, 328, and 634 are located in this region. MAb 534 was deduced to react with discontinuous CP epitope. Results of analogic peptide synthesis for the MAb 151 epitope indicate that two lysine residues are essential for binding of this MAb to its epitope. Our polyclonal antibodies against PVA reacted with six different regions in this part of the CP.","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"5 1","pages":"221-226"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87328092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500415
L. Ciuffetti, L. Francl, G. M. Ballance, W. Bockus, L. Lamari, S. Meinhardt, J. Rasmussen
{"title":"Standardization of toxin nomenclature in the Pyrenophora tritici-repentis/wheat interaction","authors":"L. Ciuffetti, L. Francl, G. M. Ballance, W. Bockus, L. Lamari, S. Meinhardt, J. Rasmussen","doi":"10.1080/07060669809500415","DOIUrl":"https://doi.org/10.1080/07060669809500415","url":null,"abstract":"","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"21 3","pages":"421-424"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72599125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500416
G. M. Ballance, L. Lamar
{"title":"Molecular aspects of host-pathogen interactions in tan spot of wheat","authors":"G. M. Ballance, L. Lamar","doi":"10.1080/07060669809500416","DOIUrl":"https://doi.org/10.1080/07060669809500416","url":null,"abstract":"","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"45 1","pages":"425-427"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88101099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500406
M. Fernandez, B. McConkey, R. Zentner
{"title":"Tillage and summerfallow effects on leaf spot diseases of wheat in the semiarid Canadian Prairies","authors":"M. Fernandez, B. McConkey, R. Zentner","doi":"10.1080/07060669809500406","DOIUrl":"https://doi.org/10.1080/07060669809500406","url":null,"abstract":"","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"46 1","pages":"376-379"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90691821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500412
F. Gamba, L. Lamari
{"title":"Mendelian inheritance of resistance to tan spot [Pyrenophora tritici-repentis] in selected genotypes of durum wheat (Triticum turgidum)","authors":"F. Gamba, L. Lamari","doi":"10.1080/07060669809500412","DOIUrl":"https://doi.org/10.1080/07060669809500412","url":null,"abstract":"","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"183 1","pages":"408-414"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88469449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500398
A. Ekramoddoullah, Y. Tan
Foliar and stem proteins from 21 resistant and 21 susceptible sugar pine seedlings inoculated with Cronartium ribicola were resolved on SDS-PAGE and two-dimensional gel electrophoresis. The protein profiles of pooled and individual samples were analyzed. SDS-PAGE analysis showed the presence of a 19-kDa protein band in infected susceptible foliage. Partial N-terminal amino acid sequence analysis revealed that this band was a mixture of histone and a Cro r I protein. The Cro r I protein was fungus- derived, while the origin of histone was not clear. Utilizing fungal-specific monoclonal antibodies, we determined that about 90% of susceptible seedlings had fungal antigens, while only one resistant seedling showed the presence of fungal antigens. This illustrated the potential use of these monoclonal antibodies as probes in screening seedlings for susceptibility. In addition, significantly greater amounts of seasonally regulated Pin I I protein was found in the foliage of resistant versus susceptible seedling...
采用SDS-PAGE和双向凝胶电泳技术，对21株抗性和21株易感糖松苗的叶片和茎部蛋白进行了分离。分析了混合样品和单个样品的蛋白质谱。SDS-PAGE分析显示，受感染的易感叶片中存在一条19 kda的蛋白带。部分n端氨基酸序列分析表明，该条带是组蛋白和Cro r I蛋白的混合物。Cro r I蛋白来源于真菌，而组蛋白的来源尚不清楚。利用真菌特异性单克隆抗体，我们确定约90%的易感幼苗具有真菌抗原，而只有一株抗性幼苗显示真菌抗原的存在。这说明了这些单克隆抗体作为筛选幼苗易感性的探针的潜在用途。此外，抗性幼苗叶片中季节性调节的Pin I I蛋白含量显著高于易感幼苗。
{"title":"Differential accumulation of proteins in resistant and susceptible sugar pine (Pinus lambertiana) seedlings inoculated with the white pine blister rust fungus (Cronartium ribicola)","authors":"A. Ekramoddoullah, Y. Tan","doi":"10.1080/07060669809500398","DOIUrl":"https://doi.org/10.1080/07060669809500398","url":null,"abstract":"Foliar and stem proteins from 21 resistant and 21 susceptible sugar pine seedlings inoculated with Cronartium ribicola were resolved on SDS-PAGE and two-dimensional gel electrophoresis. The protein profiles of pooled and individual samples were analyzed. SDS-PAGE analysis showed the presence of a 19-kDa protein band in infected susceptible foliage. Partial N-terminal amino acid sequence analysis revealed that this band was a mixture of histone and a Cro r I protein. The Cro r I protein was fungus- derived, while the origin of histone was not clear. Utilizing fungal-specific monoclonal antibodies, we determined that about 90% of susceptible seedlings had fungal antigens, while only one resistant seedling showed the presence of fungal antigens. This illustrated the potential use of these monoclonal antibodies as probes in screening seedlings for susceptibility. In addition, significantly greater amounts of seasonally regulated Pin I I protein was found in the foliage of resistant versus susceptible seedling...","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"20 1","pages":"308-318"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81951807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1998-12-01DOI: 10.1080/07060669809500411
F. Gamba, L. Lamari, A. Brûlé-Babel
{"title":"INHERITANCE OF RACE-SPECIFIC NECROTIC AND CHLOROTIC REACTIONS INDUCED BY PYRENOPHORA TRITICI-REPENTIS IN HEXAPLOID WHEATS","authors":"F. Gamba, L. Lamari, A. Brûlé-Babel","doi":"10.1080/07060669809500411","DOIUrl":"https://doi.org/10.1080/07060669809500411","url":null,"abstract":"","PeriodicalId":9607,"journal":{"name":"Canadian Journal of Plant Pathology-revue Canadienne De Phytopathologie","volume":"9 1","pages":"401-407"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85571797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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