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Exogenous GABA stimulates endogenous GABA and phenolic acid contents in tomato plants under salt stress 外源GABA刺激盐胁迫下番茄内源GABA和酚酸含量
Pub Date : 2018-03-30 DOI: 10.18466/CBAYARFBE.348935
F. Çekiç
Gamma aminobutyric acid (GABA) is a non protein amino acid found in various organisms including plants. In recent years, the signal role of GABA in the stress response has of special interest. However, the effects of exogenous GABA on phenolic compounds which have special roles as antioxidants are not well known. In this study, the effects of exogenous GABA on endogenous GABA and phenolic contents were analyzed in tomato plants under salt stress. Tomato plants were grown in a growth chamber under controlled conditions and NaCl and GABA were applied in Hoagland solution. Qualitative and quantitative analysis of GABA and phenolic compounds (Benzoic, caffeic, chlorogenic, gallic, hydroxybenzoic, syringic, rosmarinic, p-coumaric, sinapic, t-cinnamic, t-ferulic acids, catechin, epicatechin, hesperidin and quercetin) were measured by HPLC. Differences were found in chlorogenic acid, coumaric acid and gallic acid among the phenolic substances. We found a significant increase in gallic acid and coumaric acid contents under 200 mM NaCl and GABA applications. Exogenous GABA treatment caused a slight increase in endogenous GABA content. The increase in GABA content under GABA+salt treatments were higher than that of single salt and GABA applications. According to our results we can suggest that exogenous GABA could enhance the stress response by enhancing some phenolic substances and GABA content under salt stress.
γ氨基丁酸(GABA)是一种非蛋白质氨基酸,存在于包括植物在内的各种生物体中。近年来,GABA在应激反应中的信号作用引起了人们的特别关注。然而,外源GABA对具有特殊抗氧化作用的酚类化合物的影响尚不清楚。本研究分析了盐胁迫下外源GABA对番茄内源GABA和酚类物质含量的影响。在可控条件下,在生长室内培养番茄植株,在Hoagland溶液中添加NaCl和GABA。采用高效液相色谱法对GABA和酚类化合物(苯甲酸、咖啡酸、绿原酸、没食子酸、羟基苯甲酸、丁香酸、迷迭香酸、对香豆酸、辛酸、t-肉桂酸、t-阿魏酸、儿茶素、表儿茶素、橙皮苷、槲皮素)进行定性和定量分析。酚类物质中绿原酸、香豆酸和没食子酸含量存在差异。在200 mM NaCl和GABA处理下,没食子酸和香豆酸含量显著增加。外源GABA处理使内源GABA含量略有增加。GABA+盐处理的GABA含量增幅高于单盐处理和GABA处理。根据本研究结果,外源GABA可以通过提高盐胁迫下某些酚类物质和GABA含量来增强胁迫反应。
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引用次数: 11
Automatic Object Painting with SCARA Robot Using Computer Vision 基于计算机视觉的SCARA机器人自动对象绘制
Pub Date : 2018-03-30 DOI: 10.18466/cbayarfbe.306950
Ibrahim Kesici, Şeyma Bişkin, Alaa Eleyan
Recognizing and accurately classifying colors in industrial applications is a critical challenge in developing robotics painting applications. To achieve this, many painting robots are attached with expensive color sensors. However, these sensors are coming with many drawbacks such as color ranges limitation and sensitivity to illumination in addition to their high costs. In the last decades, camera systems gained importance in robotics applications with the power presented by the computer vision techniques. The main objective of this paper is to design an automation line that includes a robot and camera system to perform painting in different colors, with various illumination conditions at cheaper costs. The proposed system can be used to paint multiple colors effectively and accurately. The power of the system comes from the color detection and classification algorithm that is designed using computer vision techniques. The algorithm is designed under C++ environment using OpenCV library. The system will able to detect all colors that are adjusted/predefined offline by the user and to work in different illumination conditions. The end-effector of the robot consists of two main parts, a camera to detect the desired color and an automatic spray gun to perform the painting operation. The proposed system detects will do so based on a small sticker pasted on the object that will be painted. When the desired color is detected, the system starts the painting operation. Moreover, the system has the capability to automatically cleaning the spray gun and the connected tubes in the case of having the successive objects to be painted in different colors.
在工业应用中识别和准确分类颜色是开发机器人绘画应用的关键挑战。为了实现这一目标,许多绘画机器人都安装了昂贵的颜色传感器。然而,这些传感器除了成本高外,还有许多缺点,如颜色范围限制和对照明的敏感性。在过去的几十年里,随着计算机视觉技术的发展,相机系统在机器人应用中变得越来越重要。本文的主要目的是设计一条自动化生产线,其中包括一个机器人和相机系统,以较低的成本在各种照明条件下以不同的颜色进行绘画。该系统可以有效、准确地绘制多种颜色。该系统的强大功能来自于使用计算机视觉技术设计的颜色检测和分类算法。该算法是在c++环境下使用OpenCV库设计的。该系统将能够检测所有由用户离线调整/预定义的颜色,并在不同的照明条件下工作。机器人的末端执行器由两个主要部分组成,一个是检测所需颜色的摄像头,一个是进行喷涂操作的自动喷枪。所提出的系统检测将基于粘贴在将被绘制的对象上的小贴纸。当检测到所需的颜色时,系统开始绘制操作。此外,该系统具有自动清洗喷枪和连接管的能力,在连续的物体被涂成不同的颜色的情况下。
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引用次数: 2
Genome-wide EST-SSR Marker Identification in Red Wiggler Worm Eisenia fetida (Savigny, 1826) 红旋虫Eisenia fetida全基因组EST-SSR标记的鉴定(Savigny, 1826)
Pub Date : 2018-03-30 DOI: 10.18466/CBAYARFBE.390277
Vahap Eldem
Earthworms belong to the main groups of soil-dwelling invertebrates and are an important element for soil biota. The ecological importance of earthworms on terrestrial ecosystem is mainly attributed to their close synergistic interaction with soil biota. Among earthworms, Eisenia fetida has been considered as preferred for vermiculture and vermicomposting practices due to their reproductive potentials, short life cycle and wide temperature and density pressure tolerances. Although the genome and transcriptome data for E. fetida are available, the EST-SSRs composition and frequency of this species, which used for phylogenetic studies, genus-level taxonomy and population genetics, remain poorly defined. In current study, we mined publicly available transcriptome data and characterised genome-wide EST-SSR markers for this epigeic species. A total of 13,060 EST-SSRs were identified from 162,609 contigs. The most abundant EST-SSR types were found to be trimeric repeats (5,998, 46%) followed by dimeric (4,762, 36%), tetrameric (1,716, 13%), pentameric (478, 4%) and hexameric repeats (106, 1%). For SSR motifs, the most prevalent motifs were AC/GT (14.72%), followed by AT/AT (13.20%), ATC/ATG (13.03%), AAT/ATT (12.30%) and AG/CT (8.46%). Following validation of the species using COI sequences and to test the designed primer pairs, the eight primer pairs were designed, but only six of them were successfully amplified. Although EST-SSR information is scarce for annelids, the EST-SSR patterns of E. fetida seem to be similar to annelids. Overall, the EST-SSR markers help in taxonomic resolution of Eisenia genus from other earthworm genera and in studying the population structure and geographic distributions of E. fetida .
蚯蚓是土栖无脊椎动物的主要类群,是土壤生物群的重要组成部分。蚯蚓对陆地生态系统的生态重要性主要体现在蚯蚓与土壤生物群的密切协同作用上。在蚯蚓中,因其繁殖能力强、生命周期短、对温度和密度压力的耐受性广而被认为是蚯蚓养殖和蚯蚓堆肥的首选。尽管已经获得了fetida的基因组和转录组数据,但用于系统发育研究、属级分类和群体遗传学的EST-SSRs组成和频率仍然不明确。在目前的研究中,我们挖掘了公开可用的转录组数据,并对该表观遗传物种的全基因组EST-SSR标记进行了表征。从162,609个contigs中共鉴定出13,060个est - ssr。最丰富的EST-SSR类型是三聚体(5,998,46%),其次是二聚体(4,762,36%)、四聚体(1,716,13%)、五聚体(478,4%)和六聚体(106,1%)。SSR基序以AC/GT为主(14.72%),其次为AT/AT(13.20%)、ATC/ATG(13.03%)、AAT/ATT(12.30%)和AG/CT(8.46%)。利用COI序列对物种进行验证,并对设计的引物对进行检验,共设计了8对引物,但扩增成功的引物对只有6对。虽然关于环节动物的EST-SSR信息很少,但fetida的EST-SSR模式似乎与环节动物相似。综上所述,EST-SSR标记有助于将Eisenia属与其他蚯蚓属区分开来,并有助于研究fetida的种群结构和地理分布。
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引用次数: 0
Transfer Hydrogenation of Ketones Using Pyridine-Aminoalcohol Ligands 吡啶-氨基醇配体对酮的转移加氢反应
Pub Date : 2018-03-30 DOI: 10.18466/CBAYARFBE.373599
Serpil Denizaltı
Pyridine-aminoalcohol ligands were synthesized and evaluated as catalyst generated in situ from RuCl 2 (PPh 3 ) 3 for transfer hydrogenation of aromatic ketones. It was found that the ligand I was more active than II.
以RuCl 2 (PPh 3) 3为催化剂,合成了吡啶-氨基醇配体,并对其在芳香酮转移加氢反应中的催化作用进行了评价。发现配体I比II更有活性。
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引用次数: 0
Development of a Direct Trypan Blue Exclusion Method to Detect Cell Viability of Adherent Cells into ELISA Plates 直接台盼蓝排除法检测贴壁细胞活力的建立
Pub Date : 2018-03-30 DOI: 10.18466/CBAYARFBE.372192
Selcen Çelik Uzuner
Cell viability detection is important in cell culture applications including measurement of cell proliferation i.e for understanding cytotoxic effects of compounds on cells. There are some cell viability methods based on fluorescence or non-fluorescence detection. More simplified evaluation for cell viability, such as trypan blue staining, can be preferred before performing fluorescence assays. This appears advantageous when to have a large number of cell samples in ELISA plates after treatments with different concentrations of drug candidates. Thus, further fluorescence assays can include less concentrations rather than experiencing all used along 96-well plates. For this, trypan blue exclusion method is an option. Traditionally, treated cells are harvested by centrifugation and incubated with trypan blue within tubes followed by transferring the mixture into a hemacytometer with two chambers and assessed under the microscope. Nevertheless, using a hemacytometer limits practicability of this method when analyzing various cell samples into 96-well plates at the same time. This study was aimed to adapt trypan blue method to in situ staining of adherent cells cultured on ELISA plates. For this, cells were fixed with different fixatives after trypan blue incubation to maintain cells in impenetrable meshwork, and paraformaldehyde was the most effective fixative. This modified protocol was validated by testing the effect of dimethylsulfoxide-a cytotoxic agent-on cells, and expectedly found that cell viability reduced with higher concentrations of dimethylsulfoxide suggesting that in situ detection of cell viability by trypan blue can be a useful tool for preliminary detection of cells cultured on ELISA plates before performing automatized experiments with such flow cytometer and/or microplate reader.
细胞活力检测在细胞培养应用中很重要,包括测量细胞增殖,即了解化合物对细胞的细胞毒性作用。有一些基于荧光或非荧光检测的细胞活力检测方法。更简单的细胞活力评估,如台盼蓝染色,可优先进行荧光分析之前。当用不同浓度的候选药物处理后,在ELISA板中有大量细胞样本时,这似乎是有利的。因此,进一步的荧光分析可以包括更少的浓度,而不是经历沿96孔板全部使用。为此,台盼蓝排除法是一种选择。传统上,处理后的细胞通过离心和台盼蓝在试管中孵育,然后将混合物转移到具有两个腔室的血细胞计中,并在显微镜下进行评估。然而,当同时分析96孔板上的各种细胞样品时,使用血细胞计限制了该方法的实用性。本研究旨在使台盼蓝法适用于ELISA板上培养的贴壁细胞的原位染色。为此,在台盼蓝培养后,用不同的固定剂固定细胞,使细胞保持在不可穿透的网络中,多聚甲醛是最有效的固定剂。通过测试二甲亚砜(一种细胞毒性物质)对细胞的影响,该改进方案得到了验证,并预期发现细胞活力随着二甲亚砜浓度的升高而降低,这表明在流式细胞仪和/或微孔板阅读器进行自动化实验之前,用台色蓝原位检测细胞活力可以作为在ELISA板上培养的细胞进行初步检测的有用工具。
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引用次数: 12
Electrospun Nanofibers prepared with CdTe QDs, CdTeSe QDs and CdTe/CdS Core-shell QDs CdTe量子点、CdTeSe量子点和CdTe/CdS核壳量子点制备的电纺丝纳米纤维
Pub Date : 2018-03-30 DOI: 10.18466/CBAYARFBE.373679
C. Baslak, Ozcan Koysuren, M. Kuş
In this study, a novel method is used to prepare water-soluble CdTe, CdTeSe quantum dots (QDS) and CdTe/CdS core-shell QDs by using Te, Se, NaBH 4 , and CdCl 2 as precursors. Electrospinning method is used to obtain polyvinyl alcohol (PVA)/QDs composite nanofibers. A series of PVA/QDs nanofiber mats with three different QDs content are prepared. Transmission electron microscopy (TEM) and X-ray powder diffraction (XRD) have been used to characterize QD nanoparticles. In addition, for characterization of the composite nanofibers, scanning electron microscopy (SEM) and atomic force microscopy (AFM) have been used. QDs have a good dispersed crystalline structure with an average crystalline size between 2-4 nm. Morphology analyses reveal that the composite nanofibers with an average diameter ranging from 50 to 250 nm are prepared using the electrospinning technique.
本研究以Te、Se、NaBH 4和CdCl 2为前驱体,制备了水溶性CdTe、CdTeSe量子点(QDS)和CdTe/CdS核壳量子点。采用静电纺丝法制备了聚乙烯醇(PVA)/量子点复合纳米纤维。制备了具有三种不同量子点含量的PVA/QDs纳米纤维垫。利用透射电子显微镜(TEM)和x射线粉末衍射仪(XRD)对QD纳米颗粒进行了表征。此外,利用扫描电子显微镜(SEM)和原子力显微镜(AFM)对复合纳米纤维进行了表征。量子点具有良好的分散晶体结构,平均晶粒尺寸在2 ~ 4 nm之间。形貌分析表明,采用静电纺丝技术制备的复合纳米纤维的平均直径在50 ~ 250 nm之间。
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引用次数: 0
Synthesis and Characterization of Benzimidazolium Salts Bearing Triazole Groups 含三唑基苯并咪唑盐的合成与表征
Pub Date : 2018-03-30 DOI: 10.18466/CBAYARFBE.346566
D. Atlı
A propargyl-functionalized salt, 1-(2-methoxyethyl)-3-(prop-2-ynyl)benzimidazolium bromide ( 1 ), was prepared. 1-(2-methoxyethyl)-3-[(1-phenyl-1H-1,2,3-triazol-4-yl)methyl]benzimidazolium hexafluorophos- phate ( 2 ) and 1-(2-methoxyethyl)-3-[(1-(4-nitrophenyl)-1H-1,2,3-triazol-4-yl)methyl]benzimidazolium hexafluorophosphate ( 3 ) were obtained by Cu(I) catalyzed azide-alkyne cycloaddition reaction of 1 with corresponding azides. Compound 3 was transformed to 1-(2-methoxyethyl)-3-[(1-(4-aminophenyl)-1H-1,2,3-triazol-4-yl)methyl]benzimidazolium hexafluorophosphate ( 4 ) by reduction reaction with hydrazine monohydrate. The structures of these propargyl- and 1,2,3-triazolyl-functionalized benzimidazolium salts were elucidated by FT-IR, 1 H NMR, 13 C NMR and elemental analysis methods.
制备了丙炔功能化盐1-(2-甲氧基乙基)-3-(丙-2-炔基)苯并咪唑溴化盐(1)。通过Cu(I)催化叠氮化物与相应叠氮化物的环加成反应,得到1-(2-甲氧基乙基)-3-[(1-苯基- 1h -1,2,3-三唑-4-基)甲基]六氟磷苯并咪唑(2)和1-(2-甲氧基乙基)-3-[(1-(4-硝基苯基)- 1h -1,2,3-三唑-4-基)甲基]六氟磷苯并咪唑(3)。化合物3与一水合肼还原为1-(2-甲氧基乙基)-3-[(1-(4-氨基苯基)- 1h -1,2,3-三唑-4-基)甲基]苯并咪唑六氟磷酸(4)。通过FT-IR、1h NMR、13c NMR和元素分析等方法对丙炔基和1,2,3-三唑基功能化苯并咪唑盐的结构进行了表征。
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引用次数: 1
The Relationship Between The Kauffman Bracket Polynomials and The Tutte Polynomials of (2,n)-Torus Knots (2,n)-环面结的Kauffman括号多项式与Tutte多项式的关系
Pub Date : 2017-12-29 DOI: 10.18466/CBAYARFBE.323841
A. Şahin, Abdullah Kopuzlu
In knot theory there are many important invariants that are hard to calculate. They are classified as numeric, group and polynomial invariants. These invariants contribute to the problem of classification of knots. In this study, we have done a study on the polynomial invariants of the knots. First of all, for (2,n)-torus knots which is a special class of knots, we calculated their the Kauffman bracket polynomials. We have found a general formula for these calculations. Then the Tutte polynomials of signed graphs of (2,n)-torus knots, marked with a {+} or {-} sign each on edge, have been computed. Some results have been obtained at the end of these calculations. While these researches have been studied, figures and regular diagrams of knots have been applied so much. During the first calculation, we have used skein diagrams and relations of the Kauffman polynomial. In the second calculation, the Tutte polynomials of (2,n)-torus knots have been computed and at the end of the operation some general formulas have been introduced. The signed graphs of (2,n)-torus knots have been obtained by using their regular diagrams. Then the Tutte polynomials of these graphs have been computed as a diagrammatic by recursive formulas that can be defined by deletion-contraction operations. Finally, it has been obtained that there is a relation between the Tutte polynomials and the Kauffman bracket polynomials of (2,n)-torus knots.
在结理论中有许多重要的不变量是很难计算的。它们分为数值不变量、群不变量和多项式不变量。这些不变量有助于结点的分类问题。在本研究中,我们研究了结点的多项式不变量。首先,对于(2,n)环面结点这是一类特殊的结点,我们计算了它们的考夫曼括号多项式。我们已经找到了这些计算的一般公式。然后计算(2,n)个环面结点的带符号图的Tutte多项式,每个结点在边缘上标记一个{+}或{-}符号。在这些计算的最后得到了一些结果。在这些研究中,结的图形和规则图被应用得非常多。在第一次计算中,我们使用了绞丝图和Kauffman多项式的关系。在第二次计算中,计算了(2,n)环面结点的Tutte多项式,并在运算结束时引入了一些一般公式。利用(2,n)环面结的正则图,得到了环面结的符号图。然后,这些图的Tutte多项式通过递归公式被计算为图解,该递归公式可以通过删除-收缩操作来定义。最后,得到了(2,n)-环面结点的Tutte多项式与Kauffman括号多项式之间的关系。
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引用次数: 0
Genetic diversity by AFLP analysis within Tulipa orphanidea L. (Liliaceae) populations in Manisa 马尼萨岛百合科郁金香居群遗传多样性的AFLP分析
Pub Date : 2017-12-29 DOI: 10.18466/CBAYARFBE.334831
Hande Morgil, L. Şık, Osman Erol
Tulipa orphanidea shows high morphologic diversity throughout Eastern Mediterranean. The populations of Tulipa orphanidea L. in Manisa province, are observed in terms of genetic diversity. For this purpose, leaf samples were taken from individuals in different populations and AFLP analyses were carried out by taking the related protocols into consideration. It is concluded that the Bardakci and Spil populations are genetically distant from other populations and that the individuals in these populations are in speciation process.
在整个东地中海地区,郁金香具有高度的形态多样性。对马尼萨省郁金香(Tulipa orphanidea L.)居群的遗传多样性进行了观察。为此,从不同种群的个体中采集叶片样本,并根据相关协议进行AFLP分析。Bardakci和Spil居群在遗传上与其他居群存在一定距离,居群中的个体处于物种形成过程中。
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引用次数: 0
Conversion of Diesel Vehicles to Electric Vehicles and Controlled by PID Controller 柴油车转电动车及PID控制器控制
Pub Date : 2017-12-29 DOI: 10.18466/cbayarfbe.338211
O. Mengi
Internal combustion engine vehicles are the most produced and sold vehicles on the market. In recent years, interest in electric vehicles has begun to increase, especially due to the environmental problems. In the near future, it is estimated that gasoline and diesel vehicles will be completely electric vehicles. For this reason, many studies have been conducted on electric vehicles. Particularly the change of the engine parts, the turning of the internal combustion part to the electric motor, and the design of these motors has become an important parameter. In order to improve the performances of electric vehicles, the batteries of electric motors have been improved. In this study, a simulation was carried out to conversion 1.3 JTD and 1.3 Multijet motors to electric vehicles and the performances of these forms are evaluated by comparing the motor power, torque, and speed of the two vehicles. It was tried to predict what kind of changes will be achieved by conversion these two cars into electrical forms. PID controllers were used for inspecting components. In the study including detailed calculations, the program named AVL Cruise was used. The findings have shown that the quality of the new electric vehicle equipment used instead of the internal combustion engine can also affect results. The graphs from electric vehicles were explained and compared in detail. It was observed that there may be significant differences in the efficiency of the vehicles as a result of the conversion of the internal combustion engine to the electric motor. This made the biggest difference between the two vehicles. Since there is no data related to the sale prices of vehicles in the case of the conversion internal combustion vehicles to electric vehicles, comparisons were performed only on equipment. The latest price information of the vehicles has not been discussed.
内燃机汽车是市场上生产和销售最多的汽车。近年来,人们对电动汽车的兴趣开始增加,特别是由于环境问题。在不久的将来,估计汽油和柴油汽车将完全是电动汽车。因此,人们对电动汽车进行了许多研究。特别是发动机部件的变化,内燃部分转向电动机,这些电动机的设计已成为一个重要的参数。为了提高电动汽车的性能,人们对电动机的电池进行了改进。本研究对1.3 JTD和1.3 Multijet电机转换为电动汽车进行了仿真,并通过比较两种车型的电机功率、转矩和转速来评价这两种形式的性能。它试图预测将这两辆车转换成电动形式将实现什么样的变化。采用PID控制器对部件进行检测。在包括详细计算在内的研究中,使用了AVL Cruise程序。研究结果表明,使用替代内燃机的新电动汽车设备的质量也会影响结果。对电动汽车的图形进行了详细的解释和比较。据观察,由于将内燃机转换为电动机,车辆的效率可能会有显着差异。这是两辆车之间最大的区别。由于没有与内燃机车辆转换为电动车辆的车辆销售价格有关的数据,因此只对设备进行了比较。车辆的最新价格信息尚未讨论。
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引用次数: 0
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