Pub Date : 2024-11-16DOI: 10.1016/j.ceca.2024.102971
Pablo Hernansanz-Agustín , Carmen Morales-Vidal , Enrique Calvo , Paolo Natale , Yolanda Martí-Mateos , Sara Natalia Jaroszewicz , José Luis Cabrera-Alarcón , Rebeca Acín-Pérez , Iván López-Montero , Jesús Vázquez , José Antonio Enríquez
In a comment to our recent publication, Nicholls question our results and interpretation based on theoretical arguments that reveal a profound misunderstanding of our publication.
{"title":"Electrogenic and non-electrogenic ion antiporters participate in controling membrane potential","authors":"Pablo Hernansanz-Agustín , Carmen Morales-Vidal , Enrique Calvo , Paolo Natale , Yolanda Martí-Mateos , Sara Natalia Jaroszewicz , José Luis Cabrera-Alarcón , Rebeca Acín-Pérez , Iván López-Montero , Jesús Vázquez , José Antonio Enríquez","doi":"10.1016/j.ceca.2024.102971","DOIUrl":"10.1016/j.ceca.2024.102971","url":null,"abstract":"<div><div>In a comment to our recent publication, Nicholls question our results and interpretation based on theoretical arguments that reveal a profound misunderstanding of our publication.</div></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102971"},"PeriodicalIF":4.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-09DOI: 10.1016/j.ceca.2024.102968
Mohan Manjegowda, Bimal N. Desai
{"title":"Commentary on: Li et al.; Ca2+ transients on the T cell surface trigger rapid integrin activation in a timescale of seconds. Nature Communications (2024)","authors":"Mohan Manjegowda, Bimal N. Desai","doi":"10.1016/j.ceca.2024.102968","DOIUrl":"10.1016/j.ceca.2024.102968","url":null,"abstract":"","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102968"},"PeriodicalIF":4.3,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1016/j.ceca.2024.102967
Sonja Sivcev , Stephanie Constantin , Kosara Smiljanic , Srdjan J. Sokanovic , Patrick A. Fletcher , Arthur S. Sherman , Hana Zemkova , Stanko S. Stojilkovic
The somatostatin (SST) receptor family controls pituitary hormone secretion, but the distribution and specific roles of these receptors on the excitability and voltage-gated calcium signaling of hormone producing pituitary cells have not been fully characterized. Here we show that the rat pituitary gland expressed Sstr1, Sstr2, Sstr3, and Sstr5 receptor genes in a cell type-specific manner: Sstr1 and Sstr2 in thyrotrophs, Sstr3 in gonadotrophs and lactotrophs, Sstr2, Sstr3, and Sstr5 in somatotrophs, and none in corticotrophs and melanotrophs. Most gonadotrophs and thyrotrophs spontaneously fired high-amplitude single action potentials, which were silenced by SST without affecting intracellular calcium concentrations. In contrast, lactotrophs and somatotrophs spontaneously fired low-amplitude plateau-bursting action potentials in conjunction with calcium transients, both of which were silenced by SST. Moreover, SST inhibited GPCR-induced voltage-gated calcium signaling and hormone secretion in all cell types expressing SST receptors, but the inhibition was more pronounced in somatotrophs. The pattern of inhibition of electrical activity and calcium signaling was consistent with both direct and indirect inhibition of voltage-gated calcium channels, the latter being driven by cell type-specific hyperpolarization. These results indicate that the action of SST in somatotrophs is enhanced by the expression of several types of SST receptors and their slow desensitization, that SST may play a role in the electrical resynchronization of gonadotrophs, thyrotrophs, and lactotrophs, and that the lack of SST receptors in corticotrophs and melanotrophs keeps them excitable and ready to responses to stress.
{"title":"Distribution and calcium signaling function of somatostatin receptor subtypes in rat pituitary","authors":"Sonja Sivcev , Stephanie Constantin , Kosara Smiljanic , Srdjan J. Sokanovic , Patrick A. Fletcher , Arthur S. Sherman , Hana Zemkova , Stanko S. Stojilkovic","doi":"10.1016/j.ceca.2024.102967","DOIUrl":"10.1016/j.ceca.2024.102967","url":null,"abstract":"<div><div>The somatostatin (SST) receptor family controls pituitary hormone secretion, but the distribution and specific roles of these receptors on the excitability and voltage-gated calcium signaling of hormone producing pituitary cells have not been fully characterized. Here we show that the rat pituitary gland expressed <em>Sstr1, Sstr2, Sstr3,</em> and <em>Sstr5</em> receptor genes in a cell type-specific manner: <em>Sstr1</em> and <em>Sstr2</em> in thyrotrophs, <em>Sstr3</em> in gonadotrophs and lactotrophs, <em>Sstr2, Sstr3</em>, and <em>Sstr5</em> in somatotrophs, and none in corticotrophs and melanotrophs. Most gonadotrophs and thyrotrophs spontaneously fired high-amplitude single action potentials, which were silenced by SST without affecting intracellular calcium concentrations. In contrast, lactotrophs and somatotrophs spontaneously fired low-amplitude plateau-bursting action potentials in conjunction with calcium transients, both of which were silenced by SST. Moreover, SST inhibited GPCR-induced voltage-gated calcium signaling and hormone secretion in all cell types expressing SST receptors, but the inhibition was more pronounced in somatotrophs. The pattern of inhibition of electrical activity and calcium signaling was consistent with both direct and indirect inhibition of voltage-gated calcium channels, the latter being driven by cell type-specific hyperpolarization. These results indicate that the action of SST in somatotrophs is enhanced by the expression of several types of SST receptors and their slow desensitization, that SST may play a role in the electrical resynchronization of gonadotrophs, thyrotrophs, and lactotrophs, and that the lack of SST receptors in corticotrophs and melanotrophs keeps them excitable and ready to responses to stress.</div></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102967"},"PeriodicalIF":4.3,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142614266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-29DOI: 10.1016/j.ceca.2024.102966
Ioana Stejerean-Todoran , Christine S. Gibhardt , Ivan Bogeski
The field of ferroptosis research has grown exponentially since this form of cell death was first identified over a decade ago. Ferroptosis, an iron- and ROS-dependent type of cell death, is controlled by various metabolic pathways, including but not limited to redox and calcium (Ca2+) homeostasis, iron fluxes, mitochondrial function and lipid metabolism. Importantly, therapy-resistant tumors are particularly susceptible to ferroptotic cell death, rendering ferroptosis a promising therapeutic strategy against numerous malignancies. Calcium signals are important regulators of both cancer progression and cell death, with recent studies indicating their involvement in ferroptosis. Cells undergoing ferroptosis are characterized by plasma membrane rupture and the formation of nanopores, which facilitate influx of ions such as Ca2+ into the affected cells. Furthermore, mitochondrial Ca²⁺ levels have been implicated in directly influencing the cellular response to ferroptosis. Despite the remarkable progress made in the field, our understanding of the contribution of Ca2+ signals to ferroptosis remains limited. Here, we summarize key connections between Ca²⁺ signaling and ferroptosis in cancer pathobiology and discuss their potential therapeutic significance.
{"title":"Calcium signals as regulators of ferroptosis in cancer","authors":"Ioana Stejerean-Todoran , Christine S. Gibhardt , Ivan Bogeski","doi":"10.1016/j.ceca.2024.102966","DOIUrl":"10.1016/j.ceca.2024.102966","url":null,"abstract":"<div><div>The field of ferroptosis research has grown exponentially since this form of cell death was first identified over a decade ago. Ferroptosis, an iron- and ROS-dependent type of cell death, is controlled by various metabolic pathways, including but not limited to redox and calcium (Ca<sup>2+</sup>) homeostasis, iron fluxes, mitochondrial function and lipid metabolism. Importantly, therapy-resistant tumors are particularly susceptible to ferroptotic cell death, rendering ferroptosis a promising therapeutic strategy against numerous malignancies. Calcium signals are important regulators of both cancer progression and cell death, with recent studies indicating their involvement in ferroptosis. Cells undergoing ferroptosis are characterized by plasma membrane rupture and the formation of nanopores, which facilitate influx of ions such as Ca<sup>2+</sup> into the affected cells. Furthermore, mitochondrial Ca²⁺ levels have been implicated in directly influencing the cellular response to ferroptosis. Despite the remarkable progress made in the field, our understanding of the contribution of Ca<sup>2+</sup> signals to ferroptosis remains limited. Here, we summarize key connections between Ca²⁺ signaling and ferroptosis in cancer pathobiology and discuss their potential therapeutic significance.</div></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102966"},"PeriodicalIF":4.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.ceca.2024.102962
David G. Nicholls
In a recent publication, Hernansanz-Agusti̒n et al. propose that a sodium gradient across the inner mitochondrial membrane, generated by a Na+/H+ activity integral to Complex I can account for half of the mitochondrial membrane potential. This conflicts with conventional electrophysiological and chemiosmotic understanding.
在最近发表的一篇文章中，Hernansanz-Agusti̒n 等人提出，线粒体内膜上的钠梯度是由与复合体 I 不可分割的 Na+/H+ 活性产生的，可以占线粒体膜电位的一半。这与传统的电生理学和化学渗透理解相冲突。
{"title":"Does a transmembrane sodium gradient control membrane potential in mammalian mitochondria?","authors":"David G. Nicholls","doi":"10.1016/j.ceca.2024.102962","DOIUrl":"10.1016/j.ceca.2024.102962","url":null,"abstract":"<div><div>In a recent publication, Hernansanz-Agusti̒n et al. propose that a sodium gradient across the inner mitochondrial membrane, generated by a Na<sup>+</sup>/H<sup>+</sup> activity integral to Complex I can account for half of the mitochondrial membrane potential. This conflicts with conventional electrophysiological and chemiosmotic understanding.</div></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102962"},"PeriodicalIF":4.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-21DOI: 10.1016/j.ceca.2024.102964
Felipe Tribiños, Marcelo A. Catalan
{"title":"Calcium and chloride out of sync: The role of signaling in Sjögren's salivary gland issues","authors":"Felipe Tribiños, Marcelo A. Catalan","doi":"10.1016/j.ceca.2024.102964","DOIUrl":"10.1016/j.ceca.2024.102964","url":null,"abstract":"","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102964"},"PeriodicalIF":4.3,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1016/j.ceca.2024.102961
Tadashi Makio, Junsheng Chen, Thomas Simmen
Endoplasmic reticulum (ER) stress is triggered upon the interference with oxidative protein folding that aims to produce fully folded, disulfide-bonded and glycosylated proteins, which are then competent to exit the ER. Many of the enzymes catalyzing this process require the binding of Ca2+ ions, including the chaperones BiP/GRP78, calnexin and calreticulin. The induction of ER stress with a variety of drugs interferes with chaperone Ca2+ binding, increases cytosolic Ca2+through the opening of ER Ca2+ channels, and activates store-operated Ca2+ entry (SOCE). Posttranslational modifications (PTMs) of the ER Ca2+ handling proteins through ER stress-dependent phosphorylation or oxidation control these mechanisms, as demonstrated in the case of the sarco/endoplasmic reticulum ATPase (SERCA), inositol 1,4,5 trisphosphate receptors (IP3Rs) or stromal interaction molecule 1 (STIM1). Their aim is to restore ER Ca2+ homeostasis but also to increase Ca2+ transfer from the ER to mitochondria during ER stress. This latter function boosts ER bioenergetics, but also triggers apoptosis if ER Ca2+ signaling persists. ER Ca2+ toolkit oxidative modifications upon ER stress can occur within the ER lumen or in the adjacent cytosol. Enzymes involved in this redox control include ER oxidoreductin 1 (ERO1) or the thioredoxin-family protein disulfide isomerases (PDI) and ERp57. A tight, but adaptive connection between ER Ca2+ content, ER stress and mitochondrial readouts allows for the proper functioning of many tissues, including skeletal muscle, the liver, and the pancreas, where ER stress either maintains or compromises their function, depending on its extent and context. Upon mutation of key regulators of ER Ca2+ signaling, diseases such as muscular defects (e.g., from mutated selenoprotein N, SEPN1/SELENON), or diabetes (e.g., from mutated PERK) are the result.
内质网（ER）应激是在氧化蛋白质折叠受到干扰时引发的，目的是产生完全折叠、二硫键结合和糖基化的蛋白质，然后使其能够离开ER。催化这一过程的许多酶都需要与 Ca2+ 离子结合，其中包括伴侣蛋白 BiP/GRP78、calnexin 和 calreticulin。用多种药物诱导ER应激会干扰伴侣的Ca2+结合，通过打开ER Ca2+通道增加细胞膜Ca2+，并激活贮存操作的Ca2+进入（SOCE）。ER钙离子处理蛋白的翻译后修饰（PTM）是通过ER应激依赖性磷酸化或氧化来控制这些机制的，如肌浆/内质网ATP酶（SERCA）、1,4,5-三磷酸肌醇受体（IP3Rs）或基质相互作用分子1（STIM1）的情况所示。它们的目的是恢复 ER Ca2+ 的平衡，同时在 ER 应激时增加 Ca2+ 从 ER 向线粒体的转移。后一种功能可增强 ER 的生物能，但如果 ER Ca2+ 信号持续存在，也会引发细胞凋亡。ER应激时ER Ca2+工具箱的氧化修饰可发生在ER腔内或邻近的细胞质中。参与这种氧化还原控制的酶包括ER氧化还原蛋白1（ERO1）或硫氧还原蛋白家族的蛋白二硫异构酶（PDI）和ERp57。ER Ca2+ 含量、ER 应激和线粒体读数之间存在着紧密的适应性联系，这使得包括骨骼肌、肝脏和胰腺在内的许多组织都能正常运作。一旦ER Ca2+信号的关键调节因子发生突变，就会导致肌肉缺陷（如硒蛋白N SEPN1/SELENON突变）或糖尿病（如PERK突变）等疾病。
{"title":"ER stress as a sentinel mechanism for ER Ca2+ homeostasis","authors":"Tadashi Makio, Junsheng Chen, Thomas Simmen","doi":"10.1016/j.ceca.2024.102961","DOIUrl":"10.1016/j.ceca.2024.102961","url":null,"abstract":"<div><div>Endoplasmic reticulum (ER) stress is triggered upon the interference with oxidative protein folding that aims to produce fully folded, disulfide-bonded and glycosylated proteins, which are then competent to exit the ER. Many of the enzymes catalyzing this process require the binding of Ca<sup>2+</sup> ions, including the chaperones BiP/GRP78, calnexin and calreticulin. The induction of ER stress with a variety of drugs interferes with chaperone Ca<sup>2+</sup> binding, increases cytosolic Ca<sup>2+</sup>through the opening of ER Ca<sup>2+</sup> channels, and activates store-operated Ca<sup>2+</sup> entry (SOCE). Posttranslational modifications (PTMs) of the ER Ca<sup>2+</sup> handling proteins through ER stress-dependent phosphorylation or oxidation control these mechanisms, as demonstrated in the case of the sarco/endoplasmic reticulum ATPase (SERCA), inositol 1,4,5 trisphosphate receptors (IP<sub>3</sub>Rs) or stromal interaction molecule 1 (STIM1). Their aim is to restore ER Ca<sup>2+</sup> homeostasis but also to increase Ca<sup>2+</sup> transfer from the ER to mitochondria during ER stress. This latter function boosts ER bioenergetics, but also triggers apoptosis if ER Ca<sup>2+</sup> signaling persists. ER Ca<sup>2+</sup> toolkit oxidative modifications upon ER stress can occur within the ER lumen or in the adjacent cytosol. Enzymes involved in this redox control include ER oxidoreductin 1 (ERO1) or the thioredoxin-family protein disulfide isomerases (PDI) and ERp57. A tight, but adaptive connection between ER Ca<sup>2+</sup> content, ER stress and mitochondrial readouts allows for the proper functioning of many tissues, including skeletal muscle, the liver, and the pancreas, where ER stress either maintains or compromises their function, depending on its extent and context. Upon mutation of key regulators of ER Ca<sup>2+</sup> signaling, diseases such as muscular defects (e.g., from mutated selenoprotein N, SEPN1/SELENON), or diabetes (e.g., from mutated PERK) are the result.</div></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102961"},"PeriodicalIF":4.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1016/j.ceca.2024.102963
Jan B. Parys
The IP3 receptor (IP3R) is a ubiquitously expressed Ca2+-release channel located in the endoplasmic reticulum (ER). Ca2+ signals originating from the IP3R initiate or regulate a plethora of cellular events, including cell life and death processes, e.g. exaggerated Ca2+ release from the ER to the mitochondria is a trigger for apoptosis. Recently, Cho et al. (Current Biology, 2024, DOI: 10.1016/j.cub.2024.08.057) demonstrated that in epithelial monolayers a sustained [Ca2+] elevation caused by the IP3Rs is responsible for the extrusion of adjacent apoptotic cells out of the epithelial monolayer. Interestingly, the IP3Rs involved are associated with the desmosomes via K-Ras-induced actin-interacting protein (KRAP). This study not only highlight a novel role of the IP3R in apoptosis, but also shed a new light on how KRAP -and by extension KRAP-related proteins- contribute to the regulation of IP3R activity and, more broadly, underscores the crucial role of associated proteins in determining the function of IP3Rs.
{"title":"The IP3 receptor-KRAP complex at the desmosomes: A new player in the apoptotic process","authors":"Jan B. Parys","doi":"10.1016/j.ceca.2024.102963","DOIUrl":"10.1016/j.ceca.2024.102963","url":null,"abstract":"<div><div>The IP<sub>3</sub> receptor (IP<sub>3</sub>R) is a ubiquitously expressed Ca<sup>2+</sup>-release channel located in the endoplasmic reticulum (ER). Ca<sup>2+</sup> signals originating from the IP<sub>3</sub>R initiate or regulate a plethora of cellular events, including cell life and death processes, e.g. exaggerated Ca<sup>2+</sup> release from the ER to the mitochondria is a trigger for apoptosis. Recently, Cho et al. (Current Biology, 2024, DOI: 10.1016/j.cub.2024.08.057) demonstrated that in epithelial monolayers a sustained [Ca<sup>2+</sup>] elevation caused by the IP<sub>3</sub>Rs is responsible for the extrusion of adjacent apoptotic cells out of the epithelial monolayer. Interestingly, the IP<sub>3</sub>Rs involved are associated with the desmosomes via K-Ras-induced actin-interacting protein (KRAP). This study not only highlight a novel role of the IP<sub>3</sub>R in apoptosis, but also shed a new light on how KRAP -and by extension KRAP-related proteins- contribute to the regulation of IP<sub>3</sub>R activity and, more broadly, underscores the crucial role of associated proteins in determining the function of IP<sub>3</sub>Rs.</div></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"124 ","pages":"Article 102963"},"PeriodicalIF":4.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：