The integration of multiproxy environmental data on pedogenetic processes can provide insights into the paleoclimatic evolution of a region. In this study, we associated biological markers (Phytoliths, sponge spicules, diatom frustules, and freshwater algae cysts) and organic markers (C/N ratio, stable isotopes, thermogravimetry, and FTIR) to reconstruct the genesis of Spodosols under a semi-arid climate in the Catimbau National Park (CNP). We analyzed a toposequence composed of three Spodosols in the upper positions and one Entisol in the lowest position, located at the base of the Tacaratu Formation (Brazil). This area includes environments such as the first Spodosol profile, developed under conditions prevailing at the top of the toposequence, which was directly influenced by a tectonic fault that raised the water table. PCA of Phytolith indices explained 55.3 % of the total variance: in PC1, the Phytolith assemblage indices clearly separated the wetter profile with predominant C3 vegetation (upper profile) from the drier profiles characterized by greater water stress and grass-rich vegetation, whereas PC2 highlighted the functional differences between C3 and C4 grasses. Although δ13C values in the profiles range from −11.5 to −26.3 ‰, the Phytolith indices indicate that C3-derived DOM transported from upland zones masks the underlying C4 isotopic pattern in the downslope Bh horizons. Phytolith data revealed three distinct paleoclimatic and pedogenetic stages: paludization (∼6400 years BP), fire events (2800–2150 years BP), and podzolization (2150–450 years BP). The results indicate a transition from humid, C3-dominated environments at the upper slope to drier, C4-rich settings downslope, supported by isotopic, Phytolith, and geochemical evidence of vertical and lateral podzolization controlled by slope position and water-table gradients.
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