Soil enzyme activity and microbial biomass play a critical role in myriad ecological processes. Bamboo and broad-leaved mixed forests have received widespread attention as important forestry models for ecological restoration and sustainable development; however, our understanding the influences of broad-leaved tree species in mixed bamboo forests on soil properties and microbial activity remains unclear. Here we sampled twenty-seven spatially interspersed stands of bamboo-Castanopsis chinensis Hance mixed forest (CCB), bamboo-Alniphyllum fortune (Hemsl.) Makino mixed forest (AFB), and bamboo-Choerospondias axillaris mixed forest (CAB), with different mixing ratios (0–10 %, 10–20 %, 20–40 % canopy proportions) in subtropical China, to examine the effects of tree species and mixed ratio on soil nutrients, microbial biomass, and enzyme activity. We found that the different forests exhibited variations in soil nutrient levels. CAB forests exhibit notably higher mean C, N, and P contents than AFB and CCB, particularly at a 10–20 % mixing ratio where SOC concentration reached 46.50 g/kg. CAB forests demonstrated significantly higher activities of invertase (mean 701.83U/g), urease (8393.44U/g), and catalase (501.73U/g) compared to AFB and CCB forests, with peak enzyme activities observed at a 10–20 % mixing ratio. Soil microbial biomass C and N were notably greater in CAB and CCB forests than in AFB forests. CAB forests also exhibited the highest soil microbial biomass P (mean 48.68 mg/kg), which rose consistently with an increased mixing ratio. Multiple factor analysis revealed that the enzyme activities were significantly correlated with the annual growth of fine roots in the forest and were positively correlated with resident C, total N, total P, C: N, and C: P. Overall, the results provide insights into the importance of tree species and crown size in bamboo and broadleaved tree mixed forest in soil features and the microbial activity while providing management guidance for the selection of mixed tree species for sustainable management of bamboo forest soil microenvironment.