Brigid M. Lynch PhD, Julie K. Bassett PhD, Roger L. Milne PhD, Alpa V. Patel PhD, Erika Rees-Punia PhD, I-Min Lee MBBS, ScD, Steven C. Moore PhD, Charles E. Matthews PhD
{"title":"Reply to “Reevaluating the impact of physical inactivity on cancer risk: Methodological limitations and considerations”","authors":"Brigid M. Lynch PhD, Julie K. Bassett PhD, Roger L. Milne PhD, Alpa V. Patel PhD, Erika Rees-Punia PhD, I-Min Lee MBBS, ScD, Steven C. Moore PhD, Charles E. Matthews PhD","doi":"10.1002/cncr.35875","DOIUrl":"https://doi.org/10.1002/cncr.35875","url":null,"abstract":"","PeriodicalId":138,"journal":{"name":"Cancer","volume":"131 9","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Model predictive control has recently attracted the attention of researchers in control of permanent magnet synchronous motor drives due to advantages such as simple concepts and fast dynamics. However, it is highly sensitive to changes in motor parameters and unmodelled uncertainties. To solve this problem, a model-free predictive control based on an ultra-local model is proposed in this paper, which uses only the input and output of the system based on nominal parameters. To enhance the drive performance and simplification of implementation, current control, and speed control are performed simultaneously based on the optimization of a cost function without using any PI speed or PI current controller. In the proposed method named ‛model-free predictive current and speed control′ (MFPCSC), to reduce the sensitivity to model parameters and uncertainties, a nonlinear disturbance observer (NDO) is used, which accurately estimates all uncertainties and the effect of parameters’ change. The proposed MFPCSC-NDO control method has good behaviour in tracking the speed and current reference values and has high robustness to parameter changes. The performance of the proposed method is compared with two methods of model-based predictive current control and model-free predictive current control by simulation and its behaviour is confirmed with experimental results.
模型预测控制具有概念简单、动态速度快等优点,最近引起了永磁同步电机驱动控制研究人员的关注。然而,它对电机参数变化和未建模的不确定性高度敏感。为解决这一问题,本文提出了一种基于超局部模型的无模型预测控制,它只使用基于标称参数的系统输入和输出。为了提高驱动性能和简化实施,在优化成本函数的基础上同时执行电流控制和速度控制,而不使用任何 PI 速度或 PI 电流控制器。在所提出的 "无模型预测电流和速度控制"(MFPCSC)方法中,为了降低对模型参数和不确定性的敏感性,使用了非线性干扰观测器(NDO),它能准确估计所有不确定性和参数变化的影响。所提出的 MFPCSC-NDO 控制方法在跟踪速度和电流参考值方面表现良好,对参数变化具有很高的鲁棒性。通过仿真比较了所提方法与基于模型的预测电流控制和无模型预测电流控制两种方法的性能,并用实验结果证实了其性能。
{"title":"Robust Model-Free Predictive Current and Speed Control of PMSM Drive Based on Nonlinear Disturbance Observer","authors":"Mohammad Bagher Sepahkar, Abolfazl Halvaei Niasar","doi":"10.1049/pel2.70041","DOIUrl":"https://doi.org/10.1049/pel2.70041","url":null,"abstract":"<p>Model predictive control has recently attracted the attention of researchers in control of permanent magnet synchronous motor drives due to advantages such as simple concepts and fast dynamics. However, it is highly sensitive to changes in motor parameters and unmodelled uncertainties. To solve this problem, a model-free predictive control based on an ultra-local model is proposed in this paper, which uses only the input and output of the system based on nominal parameters. To enhance the drive performance and simplification of implementation, current control, and speed control are performed simultaneously based on the optimization of a cost function without using any PI speed or PI current controller. In the proposed method named ‛model-free predictive current and speed control′ (MFPCSC), to reduce the sensitivity to model parameters and uncertainties, a nonlinear disturbance observer (NDO) is used, which accurately estimates all uncertainties and the effect of parameters’ change. The proposed MFPCSC-NDO control method has good behaviour in tracking the speed and current reference values and has high robustness to parameter changes. The performance of the proposed method is compared with two methods of model-based predictive current control and model-free predictive current control by simulation and its behaviour is confirmed with experimental results.</p>","PeriodicalId":56302,"journal":{"name":"IET Power Electronics","volume":"18 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/pel2.70041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Existing click-based interactive image segmentation methods typically initiate object extraction with the first click and iteratively refine the coarse segmentation through subsequent interactions. Unlike box-based methods, click-based approaches mitigate ambiguity when multiple targets are present within a single bounding box, but suffer from a lack of precise location and outline information. Inspired by instance segmentation, the authors propose a Generated-bbox Guided method that provides location and outline information using an automatically generated bounding box, rather than a manually labelled one, minimising the need for extensive user interaction. Building on the success of vision transformers, the authors adopt them as the network architecture to enhance model's performance. A click-based interactive image segmentation network named the Generated-bbox Guided Coarse-to-Fine Network (GCFN) was proposed. GCFN is a two-stage cascade network comprising two sub-networks: Coarsenet and Finenet. A transformer-based Box Detector was introduced to generate an initial bounding box from a inside click, that can provide location and outline information. Additionally, two feature enhancement modules guided by foreground and background information: the Foreground-Background Feature Enhancement Module (FFEM) and the Pixel Enhancement Module (PEM) were designed. The authors evaluate the GCFN method on five popular benchmark datasets and demonstrate the generalisation capability on three medical image datasets.
{"title":"The Generated-bbox Guided Interactive Image Segmentation With Vision Transformers","authors":"Shiyin Zhang, Yafei Dong, Shuang Qiu","doi":"10.1049/cvi2.70019","DOIUrl":"https://doi.org/10.1049/cvi2.70019","url":null,"abstract":"<p>Existing click-based interactive image segmentation methods typically initiate object extraction with the first click and iteratively refine the coarse segmentation through subsequent interactions. Unlike box-based methods, click-based approaches mitigate ambiguity when multiple targets are present within a single bounding box, but suffer from a lack of precise location and outline information. Inspired by instance segmentation, the authors propose a Generated-bbox Guided method that provides location and outline information using an automatically generated bounding box, rather than a manually labelled one, minimising the need for extensive user interaction. Building on the success of vision transformers, the authors adopt them as the network architecture to enhance model's performance. A click-based interactive image segmentation network named the Generated-bbox Guided Coarse-to-Fine Network (GCFN) was proposed. GCFN is a two-stage cascade network comprising two sub-networks: Coarsenet and Finenet. A transformer-based Box Detector was introduced to generate an initial bounding box from a inside click, that can provide location and outline information. Additionally, two feature enhancement modules guided by foreground and background information: the Foreground-Background Feature Enhancement Module (FFEM) and the Pixel Enhancement Module (PEM) were designed. The authors evaluate the GCFN method on five popular benchmark datasets and demonstrate the generalisation capability on three medical image datasets.</p>","PeriodicalId":56304,"journal":{"name":"IET Computer Vision","volume":"19 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/cvi2.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"计算机科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fangfang Huang, Qingfei Dai, Qingbao Ma, Kewei Zheng, Yuanyuan Wu, Di Sun, Zhongjie Yu, Yu Liu, Wei Jiang, Xiaojun Yan
Peptides have emerged as promising agents for ameliorating hyperuricemia (HUA), a condition that poses significant risks to human health. This study evaluated the HUA-alleviating potential of skipjack tuna dark muscle hydrolysate (STDH) in a mouse model of HUA induced by potassium oxonate (PO) and hypoxanthine (Hx). The results demonstrated elevated serum uric acid (SUA) levels, increased xanthine oxidase (XOD) activity in the serum and liver, and kidney and intestinal damage in HUA mice. Although the standard drug allopurinol (AP) effectively reduced SUA levels and lowered XOD activity in the serum and liver, it exacerbated kidney damage and caused significant weight loss. In contrast, STDH intervention not only significantly lowered SUA, serum creatinine (SCr), and blood urea nitrogen (BUN) levels but also inhibited XOD activity in serum and liver. Notably, STDH ameliorated renal and intestinal morphological damage, as evidenced by hematoxylin–eosin (HE) staining. Gut microbiome analysis further revealed that STDH normalized the HUA-associated elevation of the Bacteroidetes/Firmicutes ratio. Untargeted metabolomics identified STDH's regulatory effects on glycine, serine, and threonine metabolism; arginine and proline metabolism; and glycerophospholipid metabolism, with glutamine implicated as a key player in HUA pathogenesis. These findings demonstrate that STDH effectively alleviates HUA while avoiding adverse effects associated with conventional therapy, positioning it as a safe and cost-effective functional food candidate for HUA management.
{"title":"Skipjack Tuna (Katsuwonus pelamis) Dark Muscle Hydrolysate Ameliorates Hyperuricemia in Mice via Regulating Gut Microbiota and Serum Metabolism","authors":"Fangfang Huang, Qingfei Dai, Qingbao Ma, Kewei Zheng, Yuanyuan Wu, Di Sun, Zhongjie Yu, Yu Liu, Wei Jiang, Xiaojun Yan","doi":"10.1111/1750-3841.70232","DOIUrl":"https://doi.org/10.1111/1750-3841.70232","url":null,"abstract":"<div>\u0000 \u0000 <p>Peptides have emerged as promising agents for ameliorating hyperuricemia (HUA), a condition that poses significant risks to human health. This study evaluated the HUA-alleviating potential of skipjack tuna dark muscle hydrolysate (STDH) in a mouse model of HUA induced by potassium oxonate (PO) and hypoxanthine (Hx). The results demonstrated elevated serum uric acid (SUA) levels, increased xanthine oxidase (XOD) activity in the serum and liver, and kidney and intestinal damage in HUA mice. Although the standard drug allopurinol (AP) effectively reduced SUA levels and lowered XOD activity in the serum and liver, it exacerbated kidney damage and caused significant weight loss. In contrast, STDH intervention not only significantly lowered SUA, serum creatinine (SCr), and blood urea nitrogen (BUN) levels but also inhibited XOD activity in serum and liver. Notably, STDH ameliorated renal and intestinal morphological damage, as evidenced by hematoxylin–eosin (HE) staining. Gut microbiome analysis further revealed that STDH normalized the HUA-associated elevation of the Bacteroidetes/Firmicutes ratio. Untargeted metabolomics identified STDH's regulatory effects on glycine, serine, and threonine metabolism; arginine and proline metabolism; and glycerophospholipid metabolism, with glutamine implicated as a key player in HUA pathogenesis. These findings demonstrate that STDH effectively alleviates HUA while avoiding adverse effects associated with conventional therapy, positioning it as a safe and cost-effective functional food candidate for HUA management.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bamboo's susceptibility to mildew leads to contamination and economic losses, underscoring the need for effective prevention methods. This study investigated the impregnation of bamboo with ionic liquids, specifically optimizing the pressurized process of dodecylpyridinium chloride, while evaluating treatment effectiveness and leaching resistance. Techniques such as Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy-dispersive spectroscopy (EDS), and ultraviolet–visible spectroscopy (UV) were employed to analyze the distribution and bonding of dodecylpyridinium chloride within the bamboo. The optimized impregnation parameters were determined to be a concentration of 6.88 mg/mL, a duration of 98 min, and a pressure of 0.61 MPa. Treated bamboo exhibited excellent antibacterial properties and leaching resistance, achieving 100% efficacy against bamboo mildew. Furthermore, after 14 days of soaking, the retention of dodecylpyridinium chloride decreased by only 27.3%. This study highlights a sustainable method for mildew prevention, which is crucial for enhancing the durability and utility of bamboo products.
{"title":"Ionic Liquid Pretreatment Enhances Mildew Resistance in Bamboo","authors":"Qichao Bao, Chunlin Liu, Shengnan Zhang, Jiawei Zhu, Chungui Du, Jianliang Ding","doi":"10.1002/slct.202500715","DOIUrl":"https://doi.org/10.1002/slct.202500715","url":null,"abstract":"<p>Bamboo's susceptibility to mildew leads to contamination and economic losses, underscoring the need for effective prevention methods. This study investigated the impregnation of bamboo with ionic liquids, specifically optimizing the pressurized process of dodecylpyridinium chloride, while evaluating treatment effectiveness and leaching resistance. Techniques such as Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy-dispersive spectroscopy (EDS), and ultraviolet–visible spectroscopy (UV) were employed to analyze the distribution and bonding of dodecylpyridinium chloride within the bamboo. The optimized impregnation parameters were determined to be a concentration of 6.88 mg/mL, a duration of 98 min, and a pressure of 0.61 MPa. Treated bamboo exhibited excellent antibacterial properties and leaching resistance, achieving 100% efficacy against bamboo mildew. Furthermore, after 14 days of soaking, the retention of dodecylpyridinium chloride decreased by only 27.3%. This study highlights a sustainable method for mildew prevention, which is crucial for enhancing the durability and utility of bamboo products.</p>","PeriodicalId":146,"journal":{"name":"ChemistrySelect","volume":"10 16","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hyperspectral images usually lie on low-dimensional nonlinear manifolds, leading to a challenging clustering task. Deep subspace clustering–based methods have been successful in this task by converting features to linear embedding using an auto-encoder with norm. In this setting, the embedding of the auto-encoder just learns the implicit geometric structure of original samples and loses its distribution. However, the sample distribution alignment is a generalisation of sample alignment. To remedy these issues, in this article, we propose a promising method, named DSCOT, for improving subspace clustering. Specifically, we measure the reconstruction error of the auto-encoder leveraging optimal transport distance that explicitly embeds geometric distance between samples and preserves embedding distribution in observation space. It results in more appropriate embedding for subspace clustering. Several experiments on three widely used databases show that the proposed method is superior to most state-of-the-art methods.
{"title":"Deep Subspace Clustering Preserving Distribution for Hyperspectral Images","authors":"Shujun Liu, Huajun Wang","doi":"10.1049/ell2.70262","DOIUrl":"https://doi.org/10.1049/ell2.70262","url":null,"abstract":"<p>Hyperspectral images usually lie on low-dimensional nonlinear manifolds, leading to a challenging clustering task. Deep subspace clustering–based methods have been successful in this task by converting features to linear embedding using an auto-encoder with <span></span><math>\u0000 <semantics>\u0000 <msub>\u0000 <mi>ℓ</mi>\u0000 <mn>2</mn>\u0000 </msub>\u0000 <annotation>$ell _2$</annotation>\u0000 </semantics></math> norm. In this setting, the embedding of the auto-encoder just learns the implicit geometric structure of original samples and loses its distribution. However, the sample distribution alignment is a generalisation of sample alignment. To remedy these issues, in this article, we propose a promising method, named DSCOT, for improving subspace clustering. Specifically, we measure the reconstruction error of the auto-encoder leveraging optimal transport distance that explicitly embeds geometric distance between samples and preserves embedding distribution in observation space. It results in more appropriate embedding for subspace clustering. Several experiments on three widely used databases show that the proposed method is superior to most state-of-the-art methods.</p>","PeriodicalId":11556,"journal":{"name":"Electronics Letters","volume":"61 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/ell2.70262","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to evaluate the influence of burdock fructooligosaccharide (BFO) treatment on postharvest blueberries, specifically focusing on the influence of membrane lipid metabolism on overall quality. Our findings revealed that BFO treatment effectively suppressed decay index and preserved firmness in blueberry fruit. At the same time, BFO treatment effectively mitigated the production of H2O2 and malondialdehyde (MDA), while reinforcing the activities of antioxidant enzymes and enhancing the expression level. Moreover, BFO treatment preserved high amounts of unsaturated fatty acids while suppressing the gene expression and enzyme activity of phospholipase D (PLD), lipase, and lipoxygenase (LOX). In summary, the application of BFO effectively retards the metabolism of membrane lipids and preserves the integrity of cell membranes, thereby delaying blueberry senescence.
{"title":"Effects of burdock oligosaccharide preventing membrane lipid peroxidation in postharvest blueberry fruit","authors":"Jiaqi Wang, Yajuan Wang, Yuxuan Li, Runan zhao, Bingxin Sun, Yunhe Zhang, Yufeng Xu, Xuerui Yan","doi":"10.1111/1750-3841.70205","DOIUrl":"https://doi.org/10.1111/1750-3841.70205","url":null,"abstract":"<p>The aim of this study was to evaluate the influence of burdock fructooligosaccharide (BFO) treatment on postharvest blueberries, specifically focusing on the influence of membrane lipid metabolism on overall quality. Our findings revealed that BFO treatment effectively suppressed decay index and preserved firmness in blueberry fruit. At the same time, BFO treatment effectively mitigated the production of H<sub>2</sub>O<sub>2</sub> and malondialdehyde (MDA), while reinforcing the activities of antioxidant enzymes and enhancing the expression level. Moreover, BFO treatment preserved high amounts of unsaturated fatty acids while suppressing the gene expression and enzyme activity of phospholipase D (PLD), lipase, and lipoxygenase (LOX). In summary, the application of BFO effectively retards the metabolism of membrane lipids and preserves the integrity of cell membranes, thereby delaying blueberry senescence.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deoxynivalenol (DON) is a pervasive ribotoxic stressor that induces intestinal epithelial barrier disruption by impairing tight junctions (TJs) and causing cellular damage. Curcumin (CUR), known for its enteroprotective properties and low toxicity, has been shown to attenuate DON-induced intestinal epithelial barrier injury. However, the underlying mechanisms are still unclear. In this study, we established in vivo and in vitro models using 30 male Kunming mice and IPEC-J2 cells to investigate the mechanisms by which CUR alleviates DON-induced intestinal epithelial barrier injury. The results showed that CUR markedly reduced DON-induced increases in intestinal permeability by restoring TJ protein expression (Claudin-4 and occludin) and preventing fiber-shaped actin (F-actin) contraction. CUR also attenuated DON-induced apoptosis by downregulating p53 and caspase activation and alleviated the G1 cell cycle arrest by reducing p21 expression. Mechanistically, CUR inhibited the activation of the ribosomal stress response (RSR)-associated p38 pathway, evidenced by decreased phosphorylation of p38, GSK3β, and ATF-2. The p38 activator dehydrocorydaline reversed CUR's protective effects. In conclusion, CUR alleviates DON-induced intestinal epithelial barrier disruption by improving RSR-associated p38 pathway-mediated TJ injury, apoptosis, and cell cycle arrest. These findings highlight the potential of CUR as a therapeutic agent for mitigating mycotoxin-induced intestinal dysfunction and suggest new avenues for drug target discovery.
{"title":"Curcumin Alleviates DON-Induced Intestinal Epithelial Barrier Disruption by Improving Ribotoxic Stress-Associated p38 Pathway-Mediated TJ Injury, Apoptosis, and Cell Cycle Arrest","authors":"Chenjiao Miao, Zuoyao Wu, Mingyu Wang, Binwen Zhang, Wangyong Yu, Yanfei Li, Zheng Cao","doi":"10.1111/1750-3841.70217","DOIUrl":"https://doi.org/10.1111/1750-3841.70217","url":null,"abstract":"<div>\u0000 \u0000 <p>Deoxynivalenol (DON) is a pervasive ribotoxic stressor that induces intestinal epithelial barrier disruption by impairing tight junctions (TJs) and causing cellular damage. Curcumin (CUR), known for its enteroprotective properties and low toxicity, has been shown to attenuate DON-induced intestinal epithelial barrier injury. However, the underlying mechanisms are still unclear. In this study, we established in vivo and in vitro models using 30 male Kunming mice and IPEC-J2 cells to investigate the mechanisms by which CUR alleviates DON-induced intestinal epithelial barrier injury. The results showed that CUR markedly reduced DON-induced increases in intestinal permeability by restoring TJ protein expression (Claudin-4 and occludin) and preventing fiber-shaped actin (F-actin) contraction. CUR also attenuated DON-induced apoptosis by downregulating p53 and caspase activation and alleviated the G1 cell cycle arrest by reducing p21 expression. Mechanistically, CUR inhibited the activation of the ribosomal stress response (RSR)-associated p38 pathway, evidenced by decreased phosphorylation of p38, GSK3β, and ATF-2. The p38 activator dehydrocorydaline reversed CUR's protective effects. In conclusion, CUR alleviates DON-induced intestinal epithelial barrier disruption by improving RSR-associated p38 pathway-mediated TJ injury, apoptosis, and cell cycle arrest. These findings highlight the potential of CUR as a therapeutic agent for mitigating mycotoxin-induced intestinal dysfunction and suggest new avenues for drug target discovery.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sanderson Dias Calixto, Thatiana Lopes Biá Ventura Simão, Marcos V. Palmeira-Mello, Gil Mendes Viana, Paloma Wetler Meireles Carreiros Assumpção, Dandara Paiva Barroso de Souza, Camila Couto do Espirito Santo, Vinicius de Oliveira Mussi, Carlos Rangel Rodrigues, Alessandra Mendonça Teles de Souza, Bárbara de A. Abrahim-Vieira, Lúcio Mendes Cabral, Elena Lasunskaia, Michelle Frazão Muzitano
Mycobacterium kansasii (Mkan) is a nontuberculous mycobacterium (NTM) commonly found in aquatic environments and is responsible for chronic pulmonary infections resembling tuberculosis. Treatment requires multiple antibiotics for at least 12 months, highlighting the challenge in managing Mkan infections. In this study, 50 thiourea derivatives were synthesized and evaluated for their cytotoxic and inhibitory effects on bacterial growth in culture and in infected macrophages using Mkan strains with varying virulence levels. In silico studies explored the structure–activity relationship (SAR) and the pharmacokinetic and toxicological profiles of the most active thiourea derivatives. As a result, 15 derivatives showed promising inhibitory activity against the reference strain, Mkan 12478. Of these, derivatives 2, 47, and 49 also significantly inhibited clinical isolates 10953 and 8835 without displaying cytotoxic effects. Furthermore, these three derivatives could inhibit intracellular mycobacterial growth in RAW 264.7 macrophages infected with strains 12478 or 8835. SAR studies revealed molecular volume and polar surface (PSA) area as important features for these thiourea derivatives, directly correlating with their antimycobacterial profile. In silico studies indicated that these compounds are potentially suitable for oral administration and have less toxicological effects than rifampicin, providing a safer alternative for antimycobacterial treatment. In conclusion, our findings suggest that thiourea derivatives 2, 47, and 49 are promising antimycobacterial agents for treating infections caused by Mkan.
{"title":"Thiourea-Based Compounds Effectiveness Against the Growth of Mycobacterium kansasii: Synthesis, Biological Activity, and Computational Analysis","authors":"Sanderson Dias Calixto, Thatiana Lopes Biá Ventura Simão, Marcos V. Palmeira-Mello, Gil Mendes Viana, Paloma Wetler Meireles Carreiros Assumpção, Dandara Paiva Barroso de Souza, Camila Couto do Espirito Santo, Vinicius de Oliveira Mussi, Carlos Rangel Rodrigues, Alessandra Mendonça Teles de Souza, Bárbara de A. Abrahim-Vieira, Lúcio Mendes Cabral, Elena Lasunskaia, Michelle Frazão Muzitano","doi":"10.1002/slct.202500596","DOIUrl":"https://doi.org/10.1002/slct.202500596","url":null,"abstract":"<p><i>Mycobacterium kansasii</i> (Mkan) is a nontuberculous mycobacterium (NTM) commonly found in aquatic environments and is responsible for chronic pulmonary infections resembling tuberculosis. Treatment requires multiple antibiotics for at least 12 months, highlighting the challenge in managing Mkan infections. In this study, 50 thiourea derivatives were synthesized and evaluated for their cytotoxic and inhibitory effects on bacterial growth in culture and in infected macrophages using Mkan strains with varying virulence levels. In silico studies explored the structure–activity relationship (SAR) and the pharmacokinetic and toxicological profiles of the most active thiourea derivatives. As a result, 15 derivatives showed promising inhibitory activity against the reference strain, Mkan 12478. Of these, derivatives <b>2</b>, <b>47</b>, and <b>49</b> also significantly inhibited clinical isolates 10953 and 8835 without displaying cytotoxic effects. Furthermore, these three derivatives could inhibit intracellular mycobacterial growth in RAW 264.7 macrophages infected with strains 12478 or 8835. SAR studies revealed molecular volume and polar surface (PSA) area as important features for these thiourea derivatives, directly correlating with their antimycobacterial profile. In silico studies indicated that these compounds are potentially suitable for oral administration and have less toxicological effects than rifampicin, providing a safer alternative for antimycobacterial treatment. In conclusion, our findings suggest that thiourea derivatives <b>2</b>, <b>47</b>, and <b>49</b> are promising antimycobacterial agents for treating infections caused by Mkan.</p>","PeriodicalId":146,"journal":{"name":"ChemistrySelect","volume":"10 16","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/slct.202500596","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gyanendra Chaudhary, Aman Verma, Bappa Manna, Akash Shrivastav, Amit Prabhakar, Deepti Verma
Potassium periodate (KIO4) catalyzed novel, efficient and highly regioselective one-pot synthesis of substituted benzimidazoles has been carried out in good to excellent yields via condensation–cyclisation reactions involving a variety of aldehydes and o-phenylenediamines. As a catalyst, KIO4 shows good compatibility with various substrates, easy removal, easy handling, operational simplicity, and work in mild reaction conditions. A comparative study, microwave (MW) versus conventional method, for synthesizing 2-substituted benzimidazoles via KIO4 catalysis is also described. It was observed that the microwave irradiation approach improves the yield and minimizes time (from hour to min), and represents more advantageous eco-friendly quicker chemistry.
{"title":"Potassium Periodate Catalysis for Benzimidazoles Synthesis: Analysis of Microwave Irradiation Versus Conventional Methods","authors":"Gyanendra Chaudhary, Aman Verma, Bappa Manna, Akash Shrivastav, Amit Prabhakar, Deepti Verma","doi":"10.1002/slct.202501206","DOIUrl":"https://doi.org/10.1002/slct.202501206","url":null,"abstract":"<p>Potassium periodate (KIO<sub>4</sub>) catalyzed novel, efficient and highly regioselective one-pot synthesis of substituted benzimidazoles has been carried out in good to excellent yields via condensation–cyclisation reactions involving a variety of aldehydes and <i>o</i>-phenylenediamines. As a catalyst, KIO<sub>4</sub> shows good compatibility with various substrates, easy removal, easy handling, operational simplicity, and work in mild reaction conditions. A comparative study, microwave (MW) versus conventional method, for synthesizing 2-substituted benzimidazoles via KIO<sub>4</sub> catalysis is also described. It was observed that the microwave irradiation approach improves the yield and minimizes time (from hour to min), and represents more advantageous eco-friendly quicker chemistry.</p>","PeriodicalId":146,"journal":{"name":"ChemistrySelect","volume":"10 16","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}