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Strengthening of an Edge-Cracked Plate Under Bending Using Piezoelectric Actuators 弯曲作用下边缘裂纹板的压电强化
Pub Date : 2025-04-27 DOI: 10.1002/appl.70013
Sourav Pattanayak, Supriyo Roy, Prasanta Sahoo, Goutam Pohit

Monitoring structural stability and repairing damaged components has grown in importance to ensure the safety and longevity of various engineering structures. This paper presents an innovative method for improving the performance and delaying the failure of edge-cracked plate-like structures by incorporating piezoelectric actuators during bending. The proposed method uses two piezoelectric actuators strategically placed along the plate's surface to create a counter-moment that reduces the severity of the crack. The analytical model was based on well-known stress intensity factor (SIF) solutions for the cracked plate, while the SIF after actuation was calculated with the counter moment produced by the piezoelectric patch. The superposition principle was then used to determine the total SIF after repair. The proposed technique was validated against the ABAQUS-based finite element solutions. SIFs are calculated for various parameters like crack length, patch thickness, and repair voltage. The results demonstrate that the integration of piezoelectric actuators significantly enhances the beam's structural integrity by reducing the SIF. A 21.48% reduced SIF was obtained under 200 V repair voltage for a 5 mm crack length and 0.5 mm patch thickness. Overall, this novel approach offers a promising method for increasing the integrity of cracked structural components, particularly under bending, reducing maintenance costs, and enhancing overall safety.

监测结构的稳定性和修复受损构件对于保证各种工程结构的安全和寿命越来越重要。本文提出了一种利用压电致动器提高边缘裂纹类板结构弯曲性能并延缓其失效的创新方法。所提出的方法使用两个压电致动器策略性地放置在板的表面,以产生一个反矩,以减少裂纹的严重程度。分析模型基于裂纹板的应力强度因子(SIF)解,而驱动后的SIF是用压电片产生的反矩计算的。然后利用叠加原理确定修复后的总SIF。利用基于abaqus的有限元解对所提出的方法进行了验证。SIFs计算了各种参数,如裂纹长度,补丁厚度和修复电压。结果表明,压电致动器的集成通过减小SIF显著提高了梁的结构完整性。在200 V修复电压下,裂纹长度为5 mm,补片厚度为0.5 mm, SIF降低21.48%。总的来说,这种新方法提供了一种很有前途的方法,可以提高开裂结构部件的完整性,特别是在弯曲下,降低维护成本,提高整体安全性。
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引用次数: 0
Optimizing Hydrogen Direct Injection to Overcome Design Challenges in Jet Ignition Systems 优化氢直接喷射,克服喷气点火系统的设计挑战
Pub Date : 2025-04-16 DOI: 10.1002/appl.70011
Alberto Boretti

Hydrogen direct injection (DI) is a critical technology for advancing internal combustion engines (ICEs) as efficient, low-emission alternatives in a sustainable energy future. While high-pressure direct injection (HPDI) offers significant advantages in efficiency and power density—particularly when paired with jet ignition systems—it remains underdeveloped compared to port fuel injection (PFI) and low-pressure DI (LPDI) systems. This study identifies the limitations of existing HPDI injector designs, including insufficient injection pressures and mass flow rates, and emphasizes the need for single-fuel HPDI systems. A novel method utilizing cryogenic hydrogen warmed to ambient temperature in a constant-volume chamber is proposed to achieve higher injection pressures without added complexity. When integrated with jet ignition, this approach delivers brake thermal efficiencies of up to 50% across diverse operating conditions, far surpassing PFI and LPDI. The findings underscore the urgent need for investment in HPDI technology to unlock the full potential of hydrogen ICEs, enhancing efficiency, power density, and sustainability.

氢气直喷(DI)是推动内燃机(ICE)成为可持续能源未来的高效、低排放替代品的关键技术。虽然高压直喷(HPDI)在效率和功率密度方面具有显著优势,特别是与喷射点火系统搭配使用时,但与端口燃料喷射(PFI)和低压直喷(LPDI)系统相比,高压直喷系统的发展仍显不足。本研究指出了现有 HPDI 喷射器设计的局限性,包括喷射压力和质量流量不足,并强调了对单燃料 HPDI 系统的需求。该研究提出了一种新方法,利用在恒容室中加热至环境温度的低温氢气,在不增加复杂性的情况下实现更高的喷射压力。当与喷射点火相结合时,这种方法在各种工作条件下的制动热效率高达 50%,远远超过了 PFI 和 LPDI。研究结果突出表明,迫切需要对 HPDI 技术进行投资,以充分释放氢内燃机的潜力,提高效率、功率密度和可持续性。
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引用次数: 0
Realistic Modeling of Photovoltaic Solar Cell: A Simple and Accurate Two-Diode Model 光伏太阳能电池的逼真建模:一个简单而精确的双二极管模型
Pub Date : 2025-04-09 DOI: 10.1002/appl.70010
Jordan Nafack Nihako, Elie Simo, Derrick Duclos Abada Essouma, Maëlle Nanmegne Leutchouang, Christel Roseny Atteutsia Tsakem, Christelle Yolande Tchienou Tchienou, Jimy Synclair Kenhago Watia, Pierre-Olivier Logerais, Joseph Marae Djouda

Photovoltaic modules are determinant in producing sustainable energy with a reduced environmental impact. This article explores the progressive modeling of photovoltaic modules, from the straightforward but approximate one-diode model to the more accurate but more complex two-diode model. It focuses on the parameters to be considered and the judicious choice of hypotheses to obtain electrical behavior close to that obtained experimentally for different environmental conditions. A reverse coupled saturation current and the Newton−Raphson method are both used for theoretical calculation and the simulation, respectively. Simulations show that the root mean square error (RMSE) on the I–V curves is reduced by 11.2% for irradiance of 1000 W/m² and by 28.3% on the P–V curves at 60°C. Additionally, the parallel resistance estimated with the two-diode model is lower than with the single-diode model (310 to 110.8 Ω), indicating a better consideration of leakage currents. Although the computation time is increased by around 40%, the improvement in accuracy justifies this added complexity. In conclusion, the study confirms the relevance of the two-diode model for a more realistic representation of photovoltaic module performance under various environmental conditions.

光伏组件在生产可持续能源的同时减少对环境的影响是决定性的。本文探讨了光伏组件的渐进式建模,从简单但近似的单二极管模型到更精确但更复杂的双二极管模型。它的重点是要考虑的参数和明智的选择假设,以获得接近实验得到的不同环境条件下的电行为。采用反向耦合饱和电流和牛顿-拉夫森方法分别进行了理论计算和仿真。仿真结果表明,当辐照度为1000 W/m²时,I-V曲线的均方根误差(RMSE)降低了11.2%,P-V曲线在60℃时降低了28.3%。此外,双二极管模型估计的并联电阻低于单二极管模型(310至110.8 Ω),表明更好地考虑了泄漏电流。虽然计算时间增加了大约40%,但精度的提高证明了这增加的复杂性是合理的。总之,该研究证实了双二极管模型对于更真实地表示各种环境条件下光伏组件性能的相关性。
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引用次数: 0
Optimization and Scalability of Polymer-Modified PSCs Investigated by Machine Learning 基于机器学习的聚合物改性PSCs优化与可扩展性研究
Pub Date : 2025-04-06 DOI: 10.1002/appl.70009
Mohamed M. Elsenety, Christos Falaras, Elias Stathatos, Yunjuan Niu, Linhua Hu

Advanced engineering strategies are employed to optimize the performance of perovskite solar cells (PSCs). In this study, the introduction of polyvinylpyrrolidone (PVP) to the MAPbI3 perovskite precursor results in PSCs presenting self-healing ability in a moisture environment and power conversion efficiency (PCE) of up to 20.35%. We utilize machine learning to correlate comprehensive J–V experimental data with corresponding photovoltaic parameters. We identify key factors and correlations of Jsc, FF, and Voc that primarily influence the PCE and scalability of polymer-modified PSCs. The findings indicated that the correlation between PCE and active area (AE) drops from 40% in reference cells to approximately 1% in the modified cells with PVP, justifying the scale-up potential of the modified approach. This is not the case for untreated devices, where PCE is largely affected by shunt (Rsh) and series (Rs) resistances. We evaluated 25 different algorithms through cross-validation, with the Gaussian Process emerging as the best-performing model, achieving an R2 of 0.94 and minimal errors. This model/algorithm was applied to optimize the fabrication process by predicting the optimal amount of PVP, which was determined to be 4.5 mg/L, and predicting the corresponding current–voltage (J–V) characteristics as well. This study offers a robust framework for systematically designing and optimizing durable and scalable polymer-modified PSCs, advancing the field of third-generation photovoltaic technology.

人们采用先进的工程策略来优化过氧化物太阳能电池(PSCs)的性能。在本研究中,通过在 MAPbI3 包晶前驱体中引入聚乙烯吡咯烷酮(PVP),使 PSC 在潮湿环境中具有自愈能力,功率转换效率(PCE)高达 20.35%。我们利用机器学习将全面的 J-V 实验数据与相应的光伏参数关联起来。我们确定了主要影响聚合物改性 PSC 的 PCE 和可扩展性的关键因素以及 Jsc、FF 和 Voc 的相关性。研究结果表明,PCE 与有效面积 (AE) 之间的相关性从参考电池的 40% 下降到使用 PVP 改性电池的约 1%,这证明了改性方法的扩展潜力。而未经处理的设备则不然,其 PCE 主要受并联电阻 (Rsh) 和串联电阻 (Rs) 的影响。我们通过交叉验证评估了 25 种不同的算法,其中高斯过程是性能最好的模型,R2 为 0.94,误差最小。通过预测 PVP 的最佳用量(确定为 4.5 毫克/升)以及预测相应的电流-电压 (J-V) 特性,该模型/算法被用于优化制造工艺。这项研究为系统设计和优化耐用且可扩展的聚合物改性 PSCs 提供了一个稳健的框架,推动了第三代光伏技术领域的发展。
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引用次数: 0
Copper (I)-Based Metallo-Supramolecular Polymer for Nonenzymatic Electrochemical Detection of Nitrite 用于亚硝酸盐非酶电化学检测的铜基金属超分子聚合物
Pub Date : 2025-03-31 DOI: 10.1002/appl.70008
Protity Saha, Riva Akter, Nahid Naowaz, S. M. Abu Nayem, Abdul Awal, Delwar Hossain, A. J. Saleh Ahammad

The electrochemical detection of nitrite, known for its environmental and health hazards, has been a focal point of research. A novel Cu(I)-based metallo-supramolecular polymer (SMP) (polyCu) was developed for nitrite detection using a symmetrical hexadentate terpyridine ligand[4’,4”“-(1,4-phenylene) bis(2,2’:6’,2”-terpyridine)] ligand in 1:1 ratio. The Cu(I) complexation with the ligand was confirmed through UV-Vis spectroscopy, and the prepared polyCu polymer was characterized using field-emission scanning electron microscopy (FESEM) and energy dispersive X-ray spectroscopy (EDS) techniques. The viscosity measurement of polyCu was utilized to calculate its molecular weight using Mark–Houwink-Sakurada equation. Electrochemical analysis, including cyclic voltammetry (CV) and differential pulse voltammetry (DPV), revealed excellent activity in nitrite sensing, with a well-defined peak. DPV exhibited a linear range of 1–500 μM and a low limit of detection (LOD) of 2.378 μM. The sensing mechanism was predicted based on the literature review. The polyCu_GCE demonstrated high selectivity, stability, and repeatability, making it a reliable electrocatalyst. Real sample analysis affirmed its practical applicability, positioning the sensor as a cost-effective and dependable system for nitrite sensing.

由于亚硝酸盐对环境和健康的危害,其电化学检测一直是研究的热点。采用对称六齿三吡啶配体[4′,4””-(1,4-苯基)双(2,2′:6′,2”-三吡啶)]以1:1的比例制备了一种新型Cu(I)基金属超分子聚合物(polyCu),用于亚硝酸盐的检测。通过紫外可见光谱证实了Cu(I)与配体的络合作用,并利用场发射扫描电镜(FESEM)和能量色散x射线能谱(EDS)技术对所制备的多铜聚合物进行了表征。采用Mark-Houwink-Sakurada方程对聚铜的粘度进行测定,计算聚铜的分子量。电化学分析,包括循环伏安法(CV)和差分脉冲伏安法(DPV),显示出良好的亚硝酸盐传感活性,具有明确的峰。DPV的线性范围为1 ~ 500 μM,低检出限为2.378 μM。在文献综述的基础上,对其感知机制进行了预测。polyCu_GCE表现出高选择性、稳定性和可重复性,是一种可靠的电催化剂。实际样品分析证实了该传感器的实用性,将其定位为一种性价比高、可靠的亚硝酸盐传感系统。
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引用次数: 0
Effect of Microscale Fabrication on Multi-Directional Mechanical Properties of Additively Manufactured Poly Lactic Acid With Grid Infills 微尺度加工对网格填充增材制聚乳酸多向力学性能的影响
Pub Date : 2025-03-02 DOI: 10.1002/appl.70006
M. Abolfathi, F. Moroni, A. Pirondi, E. Bedogni

In additive manufacturing, infill patterns have a significant impact on both printing time and mechanical performance, creating a necessary trade-off between the two from an industrial perspective. This study aims therefore to find an easy-to-handle procedure for rapid evaluation of the influence of infill density and raster angle on the elastic properties of 3D-printed components, from the perspective of their adoption in the industrial process of component design. In particular, the study's goal is to predict the elastic modulus in three directions. Tensile tests were carried out on bulk specimens according to ISO 527 to determine the elastic properties of 3D-printed PLA necessary for the numerical analysis. Cubic specimens were then manufactured with three densities (20%, 40%, and 60%) and two raster angles (−45°/45° and 0°/90°). Quasi-static compression tests were conducted on those specimens to assess their homogenized elastic behavior in three directions. One important result of the experimental phase was the relationship between Young's modulus (E) in the three directions. The average of E in directions 1 and 2 (build plate) is named E1,2 and on the build-up directions is E3, for α = 0°/90° was E1,2 = 0.8E3 and for α = −45°/45° was E1,2 = 0.28E3. Three finite element models were developed and run with the elastic properties determined by tensile tests, namely: (a) a shell model (SHL) where the internal and external walls of the specimens were modeled using shell elements with the nominal geometry; (b) a solid model (SLD) with the nominal geometry and (c) a nonuniform section model (NUS) in which the geometry was taken from microscope image to account for manufacturing imperfections. The difference between simulation and experiment for SHL was 19%, SLD was 15%, and NUS was 13%, indicating an overall good correspondence and, at the same time, that the real geometry resulting from the manufacturing process has a non-negligible impact on the homogenized value. Besides validating the values and relationships, FEM elucidated which sections of the cubes experienced stress and contributed to stiffness under various patterns and loading scenarios.

在增材制造中,填充模式对打印时间和机械性能都有重大影响,从工业角度来看,这两者之间存在必要的权衡。因此,本研究旨在寻找一种易于操作的方法,以快速评估填充密度和光栅角度对3d打印部件弹性性能的影响,并将其应用于部件设计的工业过程中。特别是,该研究的目标是预测三个方向的弹性模量。根据ISO 527对大块试样进行拉伸试验,以确定数值分析所需的3d打印PLA的弹性性能。然后用三种密度(20%、40%和60%)和两种光栅角度(- 45°/45°和0°/90°)制作立方体样品。对这些试样进行了准静态压缩试验,以评估其在三个方向上的均匀化弹性行为。实验阶段的一个重要结果是三个方向上的杨氏模量(E)之间的关系。在1和2方向(构建板)上E的平均值命名为E1,2,在构建方向上E的平均值命名为E3,对于α = 0°/90°为E1,2 = 0.8E3,对于α = - 45°/45°为E1,2 = 0.28E3。根据拉伸试验确定的弹性特性,开发并运行了三个有限元模型,即:(a)壳模型(SHL),其中使用具有标称几何形状的壳单元对试件的内外壁进行建模;(b)具有标称几何形状的实体模型(SLD)和(c)非均匀截面模型(NUS),其中几何形状取自显微镜图像,以考虑制造缺陷。SHL的模拟和实验之间的差异为19%,SLD为15%,NUS为13%,表明总体上具有良好的对应关系,同时,制造过程产生的真实几何形状对均匀化值具有不可忽略的影响。除了验证数值和关系外,FEM还阐明了在各种模式和加载场景下立方体的哪些部分承受应力并贡献刚度。
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引用次数: 0
Cover Image: Volume 4 Issue 2 封面图片:第四卷第二期
Pub Date : 2025-02-27 DOI: 10.1002/appl.202570002

Applied Research is a multidisciplinary journal that focuses on bridging fundamental research and practical applications, supporting sustainable problem-solving and global initiatives. The journal covers high-quality research in fields such as Materials, Applied Physics, Chemistry, Applied Biology, Food Science, Engineering, Biomedical Sciences, and Social Sciences. Authors can submit various article types, including Reviews, Tutorials, and Research Articles. The journal aims to highlight innovative research that demonstrates the application of knowledge, methods, instrumentation, and technology into solutions.

《应用研究》是一本多学科期刊,专注于弥合基础研究和实际应用,支持可持续解决问题和全球倡议。该期刊涵盖了材料、应用物理、化学、应用生物学、食品科学、工程、生物医学科学和社会科学等领域的高质量研究。作者可以提交各种文章类型,包括评论、教程和研究文章。该杂志旨在突出展示知识、方法、仪器和技术在解决方案中的应用的创新研究。
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引用次数: 0
Assessing the Sustainability of Wooden Wind Turbine Blades and Towers Compared to Conventional Designs 与传统设计相比,木质风力涡轮机叶片和塔的可持续性评估
Pub Date : 2025-02-27 DOI: 10.1002/appl.70007
Alberto Boretti

This study evaluates the environmental sustainability of wooden wind turbine blades and towers in comparison to conventional materials, focusing on lifecycle CO2 emissions, production energy, and recyclability. By analyzing components made from laminated veneer lumber, the assessment reveals that wooden blades can reduce CO2 emissions by as much as 80% relative to traditional fiberglass and epoxy designs. Wooden towers, designed in modular segments, exhibit up to a 66% reduction in lifecycle emissions when compared to steel towers, along with improved transport and assembly efficiencies. These findings highlight the potential for wood-based turbine components to advance sustainable energy through reduced environmental impact and the use of renewable resources, offering a promising alternative in the transition to eco-friendly energy infrastructure.

与传统材料相比,本研究评估了木制风力涡轮机叶片和塔的环境可持续性,重点关注生命周期的二氧化碳排放、生产能源和可回收性。通过分析由层压单板木材制成的组件,评估显示,与传统的玻璃纤维和环氧树脂设计相比,木制刀片可以减少多达80%的二氧化碳排放。木塔采用模块化设计,与钢塔相比,其生命周期排放量减少了66%,同时还提高了运输和装配效率。这些发现强调了木质涡轮机组件通过减少对环境的影响和使用可再生资源来促进可持续能源的潜力,为向环保能源基础设施过渡提供了一个有前途的选择。
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引用次数: 0
Influence of Bi2O3 Concentration on Optical and Gamma Ray Shielding Properties of BaTiO3 Ceramics Bi2O3浓度对BaTiO3陶瓷光学和γ射线屏蔽性能的影响
Pub Date : 2025-02-05 DOI: 10.1002/appl.70001
Ramakumar Nodagala, Tejeswara Rao Ponnada

This study elaborated on the influence of bismuth oxide (Bi2O3, Bi) on optical and radiation shielding properties of Barium titanate (BaTiO3, BTO) when added with different wt% concentrations. To study these properties, BaTiO3–xBi2O3; x = 0,2,4,6 and 8 wt% ceramics samples were fabricated via solid state reaction method. The optical properties of prepared samples were inspected with the help of the UV–Vis technique. The absorption coefficient increased while transmittance decreased with increasing the wt% of Bi content. Samples show a decrement in indirect optical bandgap values from 3.44 to 3.35 eV while direct bandgap from 3.19 to 3.02 eV when Bi content increases from x = 0 wt% to 8 wt%. The other optical parameters, such as Urbach energy, refractive index, extinction coefficient, and dielectric constant, were also calculated. The FESEM (field emission scanning electron microscope) technique was used to identify the homogeneity in the samples. The prepared samples were tested at 356, 511, 600, 1173, 1275, and 1333 keV energies to estimate radiation shielding properties with radioactive sources 133Ba, 22Na, 137Cs, and 60Co. As Bi content increased in prepared samples, the mass attenuation coefficient (MAC) increased. At energy 356 keV, the observed MAC values are 12.685, 12.983, 13.282, 13.58, and 13.898 cm2/g while at 1333 keV, the values noticed as 5.054, 5.066, 5.079, 5.091, and 5.103 cm2/g as Bi content increased from x = 0 wt% to x = 8 wt%. Both atomic cross-section (ACS) and electronic cross-section (ECS) were calculated. ACS values are improved from 9.825 to 11.1967 barn/atom while the ECS values enhanced from 3.8949 to 4.0226 barn/electron at 356 keV as Bi content increased from x = 0 wt% to x = 8 wt%. This similar trend was observed at other energies (511, 600, 1173, and 1275 keV) for all prepared samples. The theoretical values obtained from Phy-X/PSD software were compared with calculated values and found a close agreement between them. From results, it was clear that prepared samples showed enhanced optical and radiation shielding properties when Bi content increased in BTO ceramics.

研究了不同wt%浓度的氧化铋(Bi2O3, Bi)对钛酸钡(BaTiO3, BTO)光学和辐射屏蔽性能的影响。为了研究这些性质,BaTiO3-xBi2O3;采用固相反应法制备X = 0、2、4、6、8 wt%的陶瓷样品。利用紫外可见光谱技术对制备样品的光学性质进行了检测。随着铋含量wt%的增加,吸收系数增大,透光率降低。当Bi含量从x = 0 wt%增加到8 wt%时,样品的间接带隙值从3.44减小到3.35 eV,直接带隙从3.19减小到3.02 eV。计算了其他光学参数,如厄巴赫能、折射率、消光系数和介电常数。采用场发射扫描电镜(FESEM)技术对样品进行均匀性鉴定。在356,511,600,1173,1275和1333 keV能量下测试制备的样品,以评估133Ba, 22Na, 137Cs和60Co放射源的辐射屏蔽性能。随着制备样品中Bi含量的增加,质量衰减系数(MAC)增大。在能量为356 keV时,随着Bi含量从x = 0 wt%增加到x = 8 wt%,观察到的MAC值分别为12.685、12.983、13.282、13.58和13.898 cm2/g;在1333 keV时,随着Bi含量从x = 0 wt%增加到x = 8 wt%,观察到的MAC值分别为5.054、5.066、5.079、5.091和5.103 cm2/g。计算了原子截面(ACS)和电子截面(ECS)。当Bi含量从x = 0 wt%增加到x = 8 wt%时,在356 keV下,ACS值从9.825增加到11.1967 barn/atom, ECS值从3.8949增加到4.0226 barn/electron。在其他能量(511、600、1173和1275 keV)下,对所有制备的样品也观察到类似的趋势。从Phy-X/PSD软件中得到的理论值与计算值进行了比较,发现两者非常吻合。结果表明,当BTO陶瓷中Bi含量增加时,制备的样品具有增强的光学和辐射屏蔽性能。
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引用次数: 0
A Novel Impedance Platform Based on Printed Polymer Electrodes for Automated Virus Neutralization Assays 一种基于印刷聚合物电极的新型阻抗平台用于自动病毒中和检测
Pub Date : 2025-02-05 DOI: 10.1002/appl.70004
Stefanie Michaelis, Anja Germann, Marcus Schäfer, Jannik Jungmann, Anne-Kathrin Mildner, Iris Riemann, Saskia Bast, Thorsten Knoll, Sylvia Wagner, Eike Kottkamp, Daniel Baasner, Boris Anczykowski, Joachim Wegener

Cell-based neutralization assays are of central importance for the development of new vaccine candidates as well as quality assurance of already approved vaccines. Suppression of viral infection by neutralizing antibodies present in serum of vaccinated individuals serves as an indicator for efficacy of a vaccine. Established readouts used to date are hardly automated, provide no time resolution and require expensive reagents. These shortcomings are limiting factors in vaccine development. In contrast, when virus-compatible host cells are grown on multi-electrode arrays, the cellular infection state and the associated cell response are assessable by impedance measurements. Unlike endpoint assays, the host cell response is followed continuously in real time, label-free and noninvasively. Here, a sensor platform comprising hardware, software and disposable electrode arrays is described suitable for fully automated cell-based neutralization assays tailored for high throughput screening campaigns. To develop cost-effective, disposable electrode arrays for impedance measurements, we screen printed film electrodes made from conducting polymers on the bottom of multi-well plates. The polymer electrodes were characterized for their host cell compatibility and readout performance in comparison to established gold-film electrodes. Hard- and software were tailored for robust and routine use in virological assays. Virus titration, virus neutralization as well as antiviral drug (Efavirenz) intervention studies were conducted using vesicular stomatitis virus (VSV) pseudotypes or the Env HIV-1 infectious molecular clones Ce1176 and X1632 as viral model systems. The assays showed very similar analytical performance in terms of titration curves and dose–response relationships for polymer electrodes compared to commercial gold-film electrode arrays and reporter-based endpoint assays. Considering their technical advantages over established assays, impedance readings based on low-cost polymer electrode arrays may become an attractive alternative to conventional assays using luminescent or colorimetric readouts.

基于细胞的中和试验对于开发新的候选疫苗以及已经批准的疫苗的质量保证至关重要。通过中和疫苗接种个体血清中存在的抗体来抑制病毒感染,可作为疫苗有效性的指标。迄今为止使用的读数几乎没有自动化,没有提供时间分辨率,并且需要昂贵的试剂。这些缺点是限制疫苗开发的因素。相反,当病毒兼容的宿主细胞在多电极阵列上生长时,细胞感染状态和相关的细胞反应可通过阻抗测量来评估。与终点分析不同,宿主细胞反应是实时、无标记和无创的。在这里,一个传感器平台包括硬件,软件和一次性电极阵列被描述为适合于高通量筛选活动量身定制的全自动基于细胞的中和测定。为了开发具有成本效益的一次性电极阵列用于阻抗测量,我们在多孔板底部丝网印刷了由导电聚合物制成的薄膜电极。与已建立的金膜电极相比,聚合物电极具有宿主细胞兼容性和读出性能。硬件和软件都是为在病毒学分析中可靠和常规使用而量身定制的。以水泡性口炎病毒(VSV)假型或Env HIV-1感染性分子克隆Ce1176和X1632为病毒模型系统,进行了病毒滴定、病毒中和和抗病毒药物(Efavirenz)干预研究。与商业金膜电极阵列和基于报告器的终点分析相比,该分析在滴定曲线和剂量-响应关系方面显示出非常相似的分析性能。考虑到它们相对于现有测定法的技术优势,基于低成本聚合物电极阵列的阻抗读数可能成为使用发光或比色读出的传统测定法的有吸引力的替代方法。
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