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Cytotoxicity induction by ethanolic extract of Acalypha indica loaded casein-chitosan microparticles in human prostate cancer cell line in vitro 载酪蛋白壳聚糖微颗粒的白果乙醇提取物对体外人前列腺癌细胞的细胞毒性诱导作用
Pub Date : 2014-07-01 DOI: 10.1016/j.bionut.2013.03.009
Kanchana Amarnath, Jeevitha Dhanabal, Isha Agarwal, Srividya Seshadry

The present exploration reports an extensive evaluation of in vitro anticancer efficacy of a novel aqueous ethanolic extract of Acalypha indica (ETAI) loaded chitosan-casein (CS-CT) microparticles in a cancer cell line model. Spherically shaped ETAI loaded CS-CT (CS/CT/ETAI) microparticles were prepared by colloidal coacervation technique in a completely aqueous environment with an entrapment efficiency and particle size of 85.30 ± 4.10 and 2 ± 0.96 μm respectively. Cytotoxicity, as investigated on human prostate cancer cell line (PC3) by MTT assay, revealed insignificant differences between free ETAI and CS/CT/ETAI microparticles treated cells in the first 24 h, while higher cytotoxicity was demonstrated by CS/CT/ETAI microparticles, following 72 h of incubation. Percentage of cytotoxicity also demonstrated by LDH assay, which shown the concentration dependent leakage of LDH from PC3 cells exposed to free ETAI and CS/CT/ETAI microparticles The use of significantly low concentration of Acalypha indica loaded with CS/CT is a much better approach in comparison to the use of free ETAI for cancer treatment in future.

本研究报道了一种新型负载壳聚糖-酪蛋白(CS-CT)微粒的猕猴桃水乙醇提取物(ETAI)在癌细胞系模型中的体外抗癌效果的广泛评估。采用胶体凝聚技术在全水环境下制备了球形装载ETAI的CS-CT (CS/CT/ETAI)微粒子,其包封效率为85.30±4.10 μm,粒径为2±0.96 μm。MTT法研究了游离ETAI与CS/CT/ETAI微颗粒对人前列腺癌细胞(PC3)的细胞毒性,结果表明,游离ETAI与CS/CT/ETAI微颗粒在处理前24 h的细胞毒性差异不显著,而CS/CT/ETAI微颗粒在孵育72 h后表现出更高的细胞毒性。LDH实验也证明了细胞毒性的百分比,显示了暴露于游离ETAI和CS/CT/ETAI微粒的PC3细胞中LDH的浓度依赖性泄漏。与使用游离ETAI相比,使用低浓度的装载CS/CT的白果树是一种更好的癌症治疗方法。
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引用次数: 15
Heteroaryl chalcones: Mini review about their therapeutic voyage 杂芳查尔酮的治疗历程综述
Pub Date : 2014-07-01 DOI: 10.1016/j.bionut.2014.04.003
Bijo Mathew , Jerad Suresh , Sockalingam Anbazghagan , Jayaraj Paulraj , Girish K. Krishnan

Various structural modifications of the heteroaryl chalcones templates have been made to explore its promising biological potential in recent years. This review article is an effort to sum up the design, chemistry and biological activities of heteroaryl chalcones. In this connection, we highlighted the brief summary about the therapeutic potential of chalcones which bearing heterocyclic nucleus such as furan, thiophene, thiazole, indole, benzimidazole and quinoline.

近年来,人们对杂芳基查尔酮模板进行了各种结构修饰,以探索其广阔的生物学潜力。本文综述了杂芳基查尔酮的设计、化学和生物活性。在这方面,我们重点介绍了呋喃、噻吩、噻唑、吲哚、苯并咪唑和喹啉等杂环核查尔酮的治疗潜力。
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引用次数: 51
Antioxidant activity and anticancer effect of bioactive peptide from enzymatic hydrolysate of oyster (Saccostrea cucullata) 牡蛎酶解产物生物活性肽的抗氧化活性及抗癌作用
Pub Date : 2014-07-01 DOI: 10.1016/j.bionut.2014.04.006
S. Umayaparvathi , S. Meenakshi , V. Vimalraj , M. Arumugam , G. Sivagami , T. Balasubramanian

The antioxidant and anticancer activities of bioactive peptide isolated from oyster (Saccostrea cucullata) protein hydrolysate were evaluated in vitro. The oyster hydrolysate exhibited a strong antioxidant potential as a DPPH scavenger (85.7 ± 0.37%) followed by reducing power (2.63 ± 0.2 OD at 700 nm) at a concentration of 1 mg/ml. Due to the high antioxidant potential, hydrolysate was fractionated in Sephadex G-25 gel filtration chromatography and peptides were purified by UPLC-MS. Among 7 purified peptides (SCAP1–7), 3 peptides (SCAP1, 3 and 7) had the highest scavenging ability on DPPH radicals. The amino acid sequence and molecular mass of purified peptides (SCAP1, SCAP3 and SCAP7) were Leu-Ala-Asn-Ala-Lys (MW = 515.29 Da), Pro-Ser-Leu-Val-Gly-Arg-Pro-Pro-Val-Gly-Lys-Leu-Thr-Leu (MW = 1432.89 Da) and Val-Lys-Val-Leu-Leu-Glu-His-Pro-Val-Leu (MW = 1145.75 Da), respectively. Moreover, oyster peptide SCAP1 had anticancer activity against human colon carcinoma (HT-29) cell lines. Percentage of cell growth inhibition (MTT assay), apoptotic morphological changes (AO/EtBr staining) and oxidative DNA damage (comet assay) were estimated. We thus conclude that the anticancer and antioxidative peptide (SCAP1) from oyster (S. cucullata) may be useful ingredients in pharmaceutical and nutraceutical applications.

研究了从牡蛎蛋白水解物中分离得到的生物活性肽的体外抗氧化和抗癌活性。牡蛎水解液在浓度为1 mg/ml时具有较强的DPPH清除能力(85.7±0.37%),在700 nm处还原能力(2.63±0.2 OD)。由于具有较高的抗氧化潜力,水解产物采用Sephadex G-25凝胶过滤层析,肽段采用UPLC-MS纯化。在7个纯化肽(SCAP1 - 7)中,3个肽(SCAP1、3和7)对DPPH自由基的清除能力最强。纯化肽(SCAP1、SCAP3和SCAP7)的氨基酸序列和分子量分别为Leu-Ala-Asn-Ala-Lys (MW = 515.29 Da)、pro - ser - leu - val - gly - arg - pro - pro - val - gly - lys - leu (MW = 1432.89 Da)和Val-Lys-Val-Leu-Leu-Glu-His-Pro-Val-Leu (MW = 1145.75 Da)。牡蛎肽SCAP1对人结肠癌(HT-29)细胞系具有抗肿瘤活性。估计细胞生长抑制百分比(MTT法),凋亡形态学改变百分比(AO/EtBr染色)和氧化DNA损伤百分比(彗星法)。由此可见,牡蛎(S. cucullata)的抗癌抗氧化肽(SCAP1)可能在制药和营养保健方面具有重要的应用价值。
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引用次数: 124
Modulation of microRNA 21, 125 b and 451 expression by quercetin intake and exercise in mice fed atherogenic diet 槲皮素摄入和运动对致动脉粥样硬化小鼠microRNA 21、125 b和451表达的调节
Pub Date : 2014-07-01 DOI: 10.1016/j.bionut.2014.04.005
Mahdi Garelnabi, Halleh Mahini

Introduction

Quercetin is shown to exhibits wide range of metabolic functions including its antioxidant and anti-inflammation properties. Paradoxically, exercise which induces a severe oxidative stress resulting in the depletion of plasma and tissue antioxidants is an important deterrent of CVD. We therefore hypothesized that the combination of quercetin and exercise would have favorable impact at the cellular level by augmenting antioxidant/anti-inflammatory pathways involving set of microRNAs signaling. The discovery of microRNAs (miRs), a little more than a decade ago, has dramatically changed our perspective of gene expression regulation, and has provided a unique opportunity for researchers to address some of these unexplained metabolic ambiguities.

Study design

Forty C57BL6 LDL−/− mice fed atherogenic diet. Mice were divided into four groups (10 each) as follows: control mice (NN), left untreated; control quercetin group (NQ), orally supplied with 100 μg/day of quercetin without exercising; exercise group without quercetin (NE), and exercise group with quercetin (EQ) supplements. The exercise groups were run on a treadmill for 30 minutes, 15 m/m/5 days/week for 30 days. All animals were on atherogenic diet containing 1.5% cholesterol with total 42% Fat Kcal Diet. At the end of the month of treatment, mice were sacrificed, liver, and aorta were collected for genes micRNAs analysis.

Results

miR-21 was significantly (P < 0.05) up-regulated in both liver and aorta samples, similar trend were observed in animals on exercise with or without the intake of quercetin (P < 0.05); however miR-451 was significantly (P < 0.05) down-regulated in mice livers.

Conclusion

Exercise and quercetin intake modulates the expression of miR-21, 125b and 451.

槲皮素显示出广泛的代谢功能,包括其抗氧化和抗炎症特性。矛盾的是,运动引起严重的氧化应激,导致血浆和组织抗氧化剂的消耗,这是心血管疾病的重要威慑。因此,我们假设槲皮素和运动的结合会在细胞水平上通过增加涉及一组microrna信号的抗氧化/抗炎途径产生有利的影响。十多年前,microRNAs (miRs)的发现极大地改变了我们对基因表达调控的看法,并为研究人员提供了一个独特的机会来解决这些无法解释的代谢模糊问题。40只C57BL6 LDL - / -小鼠饲喂致动脉粥样硬化饮食。小鼠分为四组,每组10只:对照组(NN),不进行治疗;槲皮素对照组(NQ),不运动,口服槲皮素100 μg/d;不添加槲皮素(NE)和添加槲皮素(EQ)的运动组。运动组在跑步机上跑步30分钟,15米/米,每周5天,连续30天。所有的动物都食用含有1.5%胆固醇和42%脂肪热量的致动脉粥样硬化饮食。治疗一个月结束时,处死小鼠,收集肝脏和主动脉进行基因micRNAs分析。结果smir -21显著(P <(P < 0.05)在肝脏和主动脉样本中上调,在运动或不摄入槲皮素的动物中也观察到类似的趋势(P <0.05);然而miR-451显著(P <0.05)在小鼠肝脏中下调。结论运动和槲皮素摄入可调节miR-21、125b和451的表达。
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引用次数: 7
Rosmarinic acid, a polyphenol, ameliorates hyperglycemia by regulating the key enzymes of carbohydrate metabolism in high fat diet – STZ induced experimental diabetes mellitus 迷迭香酸是一种多酚,它通过调节高脂饮食中碳水化合物代谢的关键酶来改善实验性糖尿病
Pub Date : 2014-07-01 DOI: 10.1016/j.bionut.2014.03.006
G. Jayanthy, S. Subramanian

Rosmarinic acid (RA) is a polyphenolic phytoconstituent found in many herbs of lamiacea species like rosemary, mint, thyme, basil, oregano. RA exhibits a wide array of benefecial and pharmacological properties including antioxidant, anti-microbial and anti-inflammatory. Oral administration of RA (100 mg/kg body weight) to high fat diet fed – low doses of STZ induced type 2 diabetic rats for 30 days established a significant (P < 0.05) decline in the levels of blood glucose, glycosylated hemoglobin, blood urea, serum uric acid and creatinine along with increase in plasma insulin level. Diminished activities of hepatospecific pathophysiological enzymes such as aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were observed in diabetic rats administered with RA. Further, the altered activities of key carbohydrate metabolizing enzymes such as hexokinase, pyruvate kinase, glucose-6-phosphatase, fructose 1,6-bisphosphatase, glucose-6-phosphate dehydrogenase, glycogen synthase and glycogen phosphorylase (P < 0.05) in the liver tissue of diabetic rats were significantly reverted to near normal levels upon treatment with RA. Also, RA administration to diabetic rats improved hepatic glycogen content suggesting the anti-hyperglycemic potential of RA in diabetic animals. The obtained results were compared with metformin, a standard oral hypoglycemic drug. Thus, the present findings indicate that RA is nontoxic and it can potentially maintain glycemic control and regulate the key enzymes of carbohydrate metabolism in experimental diabetic rats.

迷迭香酸(RA)是一种多酚类植物成分,存在于迷迭香、薄荷、百里香、罗勒、牛至等许多层叠科植物中。RA具有广泛的有益药理特性,包括抗氧化、抗微生物和抗炎。低剂量STZ诱导的2型糖尿病大鼠在高脂日粮中口服RA (100 mg/kg体重)30天,建立了显著的(P <0.05)血糖、糖化血红蛋白、尿素、血尿酸、肌酐水平下降,血浆胰岛素水平升高。糖尿病大鼠给药后肝特异性病理生理酶如谷草转氨酶(AST)、丙氨酸转氨酶(ALT)和碱性磷酸酶(ALP)活性降低。此外,葡萄糖-6-磷酸酶、果糖- 1,6-双磷酸酶、葡萄糖-6-磷酸脱氢酶、糖原合成酶和糖原磷酸化酶等关键碳水化合物代谢酶的活性也发生了变化(P <经RA治疗后,糖尿病大鼠肝组织中葡萄糖含量明显恢复到接近正常水平。此外,给予糖尿病大鼠类风湿关节炎可提高肝糖原含量,提示类风湿关节炎在糖尿病动物中的抗高血糖潜能。将所得结果与标准口服降糖药二甲双胍进行比较。因此,本研究结果表明,RA是无毒的,它可能维持血糖控制和调节实验性糖尿病大鼠碳水化合物代谢的关键酶。
{"title":"Rosmarinic acid, a polyphenol, ameliorates hyperglycemia by regulating the key enzymes of carbohydrate metabolism in high fat diet – STZ induced experimental diabetes mellitus","authors":"G. Jayanthy,&nbsp;S. Subramanian","doi":"10.1016/j.bionut.2014.03.006","DOIUrl":"10.1016/j.bionut.2014.03.006","url":null,"abstract":"<div><p><span>Rosmarinic acid (RA) is a polyphenolic phytoconstituent found in many herbs of </span><em>lamiacea</em><span> species like rosemary, mint, thyme, basil, oregano. RA exhibits a wide array of benefecial and pharmacological properties including antioxidant, anti-microbial and anti-inflammatory. Oral administration of RA (100</span> <span>mg/kg body weight) to high fat diet<span> fed – low doses of STZ induced type 2 diabetic rats for 30</span></span> <!-->days established a significant (<em>P</em> <!-->&lt;<!--> <span><span><span>0.05) decline in the levels of blood glucose, </span>glycosylated hemoglobin<span><span>, blood urea, serum </span>uric acid and creatinine along with increase in </span></span>plasma insulin level<span><span><span>. Diminished activities of hepatospecific pathophysiological enzymes such as aspartate transaminase (AST), alanine transaminase (ALT) and </span>alkaline phosphatase<span> (ALP) were observed in diabetic rats administered with RA. Further, the altered activities of key carbohydrate metabolizing enzymes such as hexokinase, </span></span>pyruvate kinase<span><span>, glucose-6-phosphatase, fructose 1,6-bisphosphatase, glucose-6-phosphate dehydrogenase, glycogen synthase and </span>glycogen phosphorylase (</span></span></span><em>P</em> <!-->&lt;<!--> <span><span>0.05) in the liver tissue of diabetic rats were significantly reverted to near normal levels upon treatment with RA. Also, RA administration to diabetic rats improved hepatic </span>glycogen content<span> suggesting the anti-hyperglycemic potential of RA in diabetic animals. The obtained results were compared with metformin<span><span>, a standard oral hypoglycemic drug. Thus, the present findings indicate that RA is nontoxic and it can potentially maintain </span>glycemic control<span> and regulate the key enzymes of carbohydrate metabolism in experimental diabetic rats.</span></span></span></span></p></div>","PeriodicalId":100182,"journal":{"name":"Biomedicine & Preventive Nutrition","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bionut.2014.03.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82109421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 52
The possible effect of diets containing fish oil (omega-3) on hematological, biochemical and histopathogical alterations of rabbit liver and kidney 含鱼油(omega-3)饲料对家兔肝脏和肾脏血液学、生化和组织病理学改变的可能影响
Pub Date : 2014-07-01 DOI: 10.1016/j.bionut.2014.03.005
Mostafa El-Moghazy , Nahla S. Zedan , Afaf M. El-Atrsh , Mohamed El-Gogary , Ehab Tousson

The dietary intake of omega-3 polyunsaturated fatty acids has emerged over the past 20 years as an important way to modify cardiovascular risk. The present study aimed to evaluate the possible effects of a partial replacement of soybean meal in control economic diet by different concentrations of fish oil on the possible harmful changes in histological structure of liver and kidney and blood parameters in rabbits. A total of 36 adult New Zealand rabbits were equally divided into four groups, (control diet and control diet supplemented with different concentrations of fish oil at levels of 0.5, 1.0 and 1.5 mL fish oil per day/kg live body weight) and dissected after 6 weeks. Our results showed that, feeding diet supplemented with fish oil were significantly increased the percentages of hemoglobin, platelets, the mean corpuscular hemoglobin, WBCs count, total proteins, albumin, albumin/globulin ratio, SGOT and testosterone and significantly decreased the total lipids, cholesterol and triglycerides. The used of fish oil are good supplements for growing rabbits without any adverse effect on histological structure of liver and kidney in rabbits.

在过去的20年中,膳食摄入omega-3多不饱和脂肪酸已成为改变心血管风险的重要途径。本研究旨在评价不同浓度鱼油部分替代对照经济饲料中的豆粕对家兔肝、肾组织结构和血液参数可能产生的有害变化的影响。将36只成年新西兰兔平均分为4组(对照组饲粮和对照组饲粮中分别添加不同浓度的鱼油,剂量分别为0.5、1.0和1.5 mL /kg活体重/天),6周后解剖。结果表明,饲粮中添加鱼油显著提高了血红蛋白、血小板、平均红细胞血红蛋白、白细胞计数、总蛋白、白蛋白、白蛋白/球蛋白比、SGOT和睾酮含量,显著降低了总脂、胆固醇和甘油三酯含量。鱼油的使用对生长兔的肝脏和肾脏的组织结构没有不良影响,是一种很好的补充。
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引用次数: 34
Diallyl sulfide attenuates renal injury by altering the expressions of COX-2 and NF-κB during cisplatin-induced nephrotoxicity in male wistar rats 二烯丙基硫醚通过改变雄性wistar大鼠顺铂肾毒性过程中COX-2和NF-κB的表达来减轻肾损伤
Pub Date : 2014-04-01 DOI: 10.1016/j.bionut.2014.01.003
Arunkumar Jagadeesan , Magendira Mani Vinayagam (Assistant Professor) , Prakash Dharmalingam

Cis-diamminedichloroplatinum (II) (cisplatin) is an effective chemotherapeutic agent successfully used in the treatment of a wide range of tumours. However, its full clinical utility is limited due to some adverse effects. Diallyl sulfide (DAS) is a major flavour component of garlic. The present study is to evaluate nephroprotective effect of DAS on the cisplatin-induced nephrotoxicity. Male Wistar rats were divided into four groups of six each: Group I served as control rats; Group II received single intraperitoneal (i.p) injection of 7 mg/kg BW cisplatin. Group III received both cisplatin and DAS (100 mg/kg BW intraperitoneally); Group IV received DAS alone. Cisplatin administration significantly alters the levels of serum marker enzymes and renal tissue markers. DAS administration significantly reduced levels of serum marker enzymes and improved renal functions. Increase MDA level with a concomitant reduction in enzymic antioxidants and non-enzymic antioxidants were observed in cisplatin-induced group, which was reversed upon DAS treatment. Cisplatin-induced nephrotoxicity which is also supported by histopathological studies and elevated expressions of nuclear transcription factor-kappa B and Cyclooxygenase-2 in cisplatin-induced group, and it were attenuated upon DAS treatment. These results indicate that the antioxidant effect of DAS might contribute against cisplatin-induced nephrotoxicity in rats.

顺式二胺二氯铂(II)(顺铂)是一种有效的化疗药物,成功地用于治疗多种肿瘤。然而,由于一些不良反应,其充分的临床应用受到限制。二烯丙基硫醚(DAS)是大蒜的主要风味成分。本研究旨在评价DAS对顺铂所致肾毒性的肾保护作用。雄性Wistar大鼠分为四组,每组6只:第一组为对照大鼠;II组患者单次腹腔注射顺铂7 mg/kg BW。III组同时给予顺铂和DAS (100 mg/kg BW腹腔注射);IV组单独接受DAS治疗。顺铂可显著改变血清标记酶和肾组织标记物的水平。服用DAS可显著降低血清标记酶水平,改善肾功能。顺铂诱导组MDA水平升高,酶促抗氧化剂和非酶促抗氧化剂同时降低,DAS治疗组则相反。顺铂诱导的肾毒性也得到了组织病理学研究的支持,顺铂诱导组的核转录因子- κ B和环氧化酶-2的表达升高,经DAS治疗后肾毒性减弱。提示DAS的抗氧化作用可能与顺铂所致大鼠肾毒性有关。
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引用次数: 4
Isolation, characterization of syringin, phenylpropanoid glycoside from Musa paradisiaca tepal extract and evaluation of its antidiabetic effect in streptozotocin-induced diabetic rats 菝葜花被提取物中紫丁香苷、苯丙苷的分离、鉴定及对链脲佐菌素诱导的糖尿病大鼠的降糖作用
Pub Date : 2014-04-01 DOI: 10.1016/j.bionut.2013.12.009
Shanmuga Sundaram Chinna Krishnan , Iyyam Pillai Subramanian , Sorimuthu Pillai Subramanian

Nature still serves as the major source for the cure of various ailments. More than 80% of people are utilizing plants as part of their routine health management. Although phytotherapy continues to be used in several countries, only few plants have received scientific or medical scrutiny. Musa species is widely distributed in tropical regions and used in folk medicine for various treatments. Recently, we have reported the antidiabetic effects of Musa paradisiaca tepal extract (MPTE) in STZ-induced diabetic rats. In the present study, we have isolated and characterized syringin, a phenyl propanoid glucoside from MPTE and evaluated its antidiabetic efficacy in streptozotocin-induced diabetic rats. Syringin was isolated from MPTE and characterized using spectral studies. Diabetic rats were administered 50 mg/kg per day syringin orally for 30 days. After experimental period, rats were sacrificed and blood was collected for important biochemical parameters such as blood glucose, insulin, hemoglobin, HbA1c, total protein, urea, uric acid and creatinine. Serum aminotransferases and alkaline phosphatases were assayed. The data revealed the presence of phenylpropanoid glycoside, syringin in MPTE. Elevated blood glucose and HbA1c levels, the reduced plasma insulin and hemoglobin levels in diabetic rats were significantly reversed to near normal after oral administration of syringin. Plasma protein, blood urea, serum creatinine and uric acid levels were also normalized after treatment. The altered activities of serum transaminases and alkaline phosphatases were normalized upon syringin treatment indicating its nontoxic nature. The presence of syringin in the tepal extract may account for its antidiabetic potential.

大自然仍然是治疗各种疾病的主要来源。超过80%的人将植物作为日常健康管理的一部分。虽然一些国家继续使用植物疗法,但只有少数植物得到了科学或医学的审查。木沙广泛分布于热带地区,在民间医药中有多种治疗作用。最近,我们报道了天堂芭蕉花瓣提取物(MPTE)对stz诱导的糖尿病大鼠的降糖作用。在本研究中,我们从MPTE中分离并鉴定了一种苯基丙酸苷丁香苷,并对其在链脲佐菌素诱导的糖尿病大鼠中的降糖作用进行了评价。从MPTE中分离得到紫丁香苷,并对其进行了光谱分析。糖尿病大鼠口服紫丁香素50 mg/kg / d,连续30 d。实验期结束后,处死大鼠,采血检测血糖、胰岛素、血红蛋白、糖化血红蛋白、总蛋白、尿素、尿酸、肌酐等重要生化指标。测定血清转氨酶和碱性磷酸酶。数据显示MPTE中存在苯丙苷、紫丁香苷。口服紫丁香苷后,糖尿病大鼠的血糖和糖化血红蛋白水平升高,血浆胰岛素和血红蛋白水平降低,明显恢复到接近正常水平。治疗后血浆蛋白、尿素、血清肌酐、尿酸水平均恢复正常。经紫丁香苷处理后,血清转氨酶和碱性磷酸酶活性的改变恢复正常,表明其无毒性质。花被提取物中紫丁香苷的存在可能是其抗糖尿病作用的原因。
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引用次数: 41
Aegle marmelos fruit extract abates dextran sodium sulfate induced acute colitis in mice: Repression of pro-inflammatory cytokines during colonic inflammation 蜜瓜提取物减轻右旋糖酐硫酸钠诱导的小鼠急性结肠炎:在结肠炎症期间抑制促炎细胞因子
Pub Date : 2014-04-01 DOI: 10.1016/j.bionut.2014.03.002
Nirmal Kumar Kasinathan, Bharathi Raja Subramaniya, Ilakkiya Pandian, Niranjali Devaraj Sivasithamparam

Ulcerative colitis (UC) is a common inflammatory bowel disease which on prolongation causes colorectal cancer (CRC) making UC as the highest risk factor for CRC development. Despite the use of Aegle marmelos in folk medicine, few studies have reported its colonic healing activity. We exploited the use of dextran sodium sulfate (DSS) in inducing colitis in Swiss albino mice and examine the inflammatory modulating effect of A. marmelos fruit extract (AME). HPLC analysis confirmed the presence of two biologically active compounds namely umbelliferon (a coumarin-derivative) and lupeol (triterpenoid). Fourteen days feeding of DSS to mice elicited colitis, with drastically reduced body weight with altered clinical severity score, combined with shortening of colon length. Oral administration of AME (50 mg/kg) evidenced a significant suppression of disease symptoms. The increased mRNA expressions of interleukin (IL) 2, IL-6 and tumor necrosis factor α during colitis, were also reduced significantly. Notable reduction in the NF-κB expression in the colonic region was also noted which is substantiates with docking analysis were UMB and LUP found in AME bounds with NF-κB. Furthermore, DSS-altered histopathological features of colon were also recovered on treatment with AME. Thus the observation revealed the restorative significance of AME in healing the DSS-induced colitis in mice by modulating NF-κB and regulating pro-inflammatory mediators involved in the colonic injury.

溃疡性结肠炎(UC)是一种常见的炎症性肠病,其长期发展可导致结直肠癌(CRC),是结直肠癌发展的最高危险因素。尽管在民间医学中使用了埃格尔甜瓜,但很少有研究报道其结肠愈合活性。我们利用葡聚糖硫酸钠(DSS)诱导瑞士白化病小鼠结肠炎,并观察甜瓜果提取物(AME)的炎症调节作用。HPLC分析证实了两种生物活性化合物的存在,即伞形花素(一种香豆素衍生物)和lupeol(三萜)。给小鼠喂食14天的DSS引起结肠炎,小鼠体重急剧下降,临床严重程度评分改变,并伴有结肠长度缩短。口服AME (50mg /kg)可显著抑制疾病症状。结肠炎期间升高的白细胞介素(IL) 2、IL-6和肿瘤坏死因子α mRNA表达量也显著降低。结肠区NF-κB的表达也显著降低,对接分析证实了在AME与NF-κB的结合中发现的UMB和LUP。此外,经AME治疗后,dss改变的结肠组织病理学特征也得以恢复。由此可见,AME通过调节NF-κB和参与结肠损伤的促炎介质,对dss诱导的小鼠结肠炎具有修复作用。
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引用次数: 7
Protective effect of selected urease positive Lactobacillus strains on acetaminophen induced uremia in rats 脲酶阳性乳杆菌对对乙酰氨基酚致大鼠尿毒症的保护作用
Pub Date : 2014-04-01 DOI: 10.1016/j.bionut.2014.02.001
Arpita Patra , Arpita Mandal , Suchismita Roy , Shreya Mandal , Keshab Chandra Mondal , Dilip Kumar Nandi

Urease positive probiotic Lactobacillus strains were tested for oxidative stress and uremic profile on experimental rat (Wister strains) induced by acetaminophen (APAP) overdose. Experimental rats received acetaminophen interperitoneally at the dose of 500 mg/kg/day, continuously for 10 days. From 11th day onwards they were orally fed with Lactobacillus fermentum (MTCC 903), Lactobacillus plantarum (MTCC 4462) and Lactobacillus rhamnosus (MTCC 1408) respectively at the dose of 109 CFU/mL/100 g of body weight/day for 15 days continuously. Plasma, kidney, liver and fecal samples were tested for uremic profile of the sacrificed rats after the experiment. In APAP treated rats, plasma urea, creatinine (Cr), glutamate oxaloacetate transaminase (GOT) and malonaldehyde (MDA) level elevated and catalase (CAT) and super oxide diusmutase (SOD) level declined significantly compared to negative control. However, level of plasma urea, Cr, GOT and MDA in tested rats were significantly lower in comparison to positive control. The uremic profile of the probiotic induced rats was very much comparable with the negative control, even better for some parametric values. Prevention of DNA fragmentation in kidney tissues and reduction of enteric pathogens in feces of Lactobacillus fed rats were noticed. Electrolytes profile of the tested plasma samples were in acceptable range. To sum up, tested urease positive Lactobacillus strains were shown to improve the clinical condition of the acetaminophen induced uremic experimental rats.

采用对乙酰氨基酚(APAP)过量诱导的实验大鼠(Wister菌株),研究了脲酶阳性益生菌乳酸杆菌的氧化应激和尿毒症特征。实验大鼠腹腔灌胃对乙酰氨基酚500 mg/kg/d,连续灌胃10 d。从第11天开始,分别以109 CFU/mL/100 g体重/天的剂量口服发酵乳杆菌(MTCC 903)、植物乳杆菌(MTCC 4462)和鼠李糖乳杆菌(MTCC 1408),连续15 d。实验结束后对处死大鼠的血浆、肾脏、肝脏和粪便进行尿毒症分析。APAP处理大鼠血浆尿素、肌酐(Cr)、谷氨酸草酰乙酸转氨酶(GOT)和丙二醛(MDA)水平显著升高,过氧化氢酶(CAT)和超氧化物歧化酶(SOD)水平显著降低。血浆尿素、铬、GOT和丙二醛水平明显低于阳性对照组。益生菌诱导大鼠的尿毒症情况与阴性对照非常相似,在某些参数值上甚至更好。观察到乳杆菌喂养大鼠可预防肾脏组织DNA断裂和减少粪便中的肠道病原体。测试血浆样品的电解质分布在可接受范围内。综上所述,脲酶阳性乳酸菌可改善对乙酰氨基酚所致尿毒症大鼠的临床状况。
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引用次数: 6
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Biomedicine & Preventive Nutrition
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