Carbohydrate Polymer Technologies and Applications最新文献_第5页

Ascorbic acid-assisted extraction of bioactive pectin from cocoa and coffee husks with antioxidant and anti-inflammatory potential 抗坏血酸辅助提取具有抗氧化和抗炎作用的可可和咖啡果胶

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101058

Sofía Mares-Bou , Damaris A. Pazmiño-Eugenio , William Cheung , Carolina I. Contreras-Monzón , Andy Hernández-Montoto , Gloria Gallego-Ferrer , Joel Girón-Hernández , Piergiorgio Gentile

Pectin, a multifunctional polysaccharide with food, pharmaceutical and biomedical applications, can be sustainably extracted from Theobroma cacao and Coffea arabica pod husk waste. This study optimized an eco-friendly, ascorbic-acid-assisted acid extraction to maximize galacturonic acid (GalA) content, achieving optimal conditions for cocoa (R= 0.02 g/mL, P= 45:55 v/v AA:HCl, T= 65 min) and coffee (R= 0.01 g/mL, P= 100:0 v/v AA, T= 65 min). The extraction yielded pectin with ≥74% GalA, meeting USP/FCC pharmaceutical-grade criteria, with cocoa and coffee husk yields of ∼7% and ∼21%, respectively. Dialysis increased purity, raising molecular weight up to ∼297,000 Da and narrowing dispersity. Extracted pectin exhibited higher total phenolic and flavonoid contents than commercial citrus pectin and showed superior antioxidant capacity (ORAC up to 53.1 mg TE/g; DPPH up to 2420 mg TE/g), supported by metabolomic profiling that identified abundant antioxidant metabolites such as protocatechuic acid, trigonelline and catechol. In vitro assays with human dermal fibroblasts demonstrated cytocompatibility at 0.1 mg/mL, mild ROS-scavenging effects and selective modulation of inflammatory cytokines, with cocoa pectin reducing IL-6 and dialyzed coffee pectin showing strong IL-6 suppression.

果胶是一种具有食品、制药和生物医学用途的多功能多糖，可以从可可树和阿拉比卡咖啡豆荚废料中可持续提取。本研究优化了一种环保的抗坏血酸辅助酸提取工艺，以最大限度地提高半乳糖醛酸（GalA）的含量，获得了可可（R= 0.02 g/mL， P= 45:55 v/v AA:HCl， T= 65 min）和咖啡（R= 0.01 g/mL， P= 100:0 v/v AA， T= 65 min）的最佳提取条件。提取得到的果胶GalA含量≥74%，符合USP/FCC药品级标准，可可和咖啡壳的得率分别为~ 7%和~ 21%。透析提高了纯度，将分子量提高到约297,000 Da，并缩小了分散性。提取的果胶比商品柑橘果胶具有更高的总酚和类黄酮含量，并具有更好的抗氧化能力（ORAC高达53.1 mg TE/g， DPPH高达2420 mg TE/g），代谢组学分析鉴定出丰富的抗氧化代谢物，如原儿茶酸、葫芦丝碱和儿茶酚。体外实验表明，在0.1 mg/mL浓度下，可可果胶可降低IL-6，咖啡果胶可明显抑制IL-6，具有轻微的ros清除作用和选择性调节炎症细胞因子。

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引用次数: 0

Nanoparticle formation of an antimicrobial peptide induced by sulfated β-cyclodextrin: Application to alkaloid encapsulation 磺化β-环糊精诱导抗菌肽纳米颗粒的形成：在生物碱包封中的应用

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101053

Zsombor Miskolczy , Mónika Megyesi , Beáta Mándityné Huszka , István Mándity , László Biczók

The association of polyanionic β-cyclodextrin (βCD) derivatives with the nonaggregating polycationic antimicrobial peptide Dhvar4 was studied in water at 296 K without a buffer to explore their potential for forming nanoparticles capable of encapsulating bioactive isoquinoline alkaloids. The βCD bearing sulfobutyl ether moieties formed a 1:1 inclusion complex with Dhvar4, exhibiting a binding constant of (1.1 ± 0.3) × 10⁵ M^–1. In contrast, βCD substituted with SO₃⁻ groups (S₁₃βCD) promoted the self-assembly into nanoparticles with a narrow and uniform size distribution. When Dhvar4 was present in large excess relative to S₁₃βCD, positively charged nanoparticles were produced. Conversely, negatively charged nanoparticles were obtained at S₁₃βCD/Dhvar4 charge ratios above 2.5, which maintained their size for at least 30 days. The incorporation of pharmaceutically active isoquinoline alkaloids had minimal impact on the properties of nanoparticles formed in the presence of a >35 molar% excess of S₁₃βCD over Dhvar4. Encapsulation efficiencies of 99 % and 72 % were achieved for coralyne and sanguinarine, respectively. Job plot and isothermal titration calorimetry measurements demonstrated that each S₁₃βCD macrocycle could bind ∼13 molecules of these alkaloids. The present findings lay the groundwork for future biological validation and applications of nanoparticles composed of three types of biomedically important compounds.

研究了聚阴离子β-环糊精（βCD）衍生物与非聚集性聚阳离子抗菌肽Dhvar4在296 K无缓冲条件下的结合，以探索它们形成能够包封生物活性异喹啉生物碱的纳米颗粒的潜力。含βCD的磺基丁基醚部分与Dhvar4形成1:1的包合物，结合常数为（1.1±0.3）× 105 M-1。相比之下，用SO₃⁻（S13βCD）取代的βCD促进了纳米粒子的自组装，使其具有窄而均匀的尺寸分布。当Dhvar4相对于S13βCD大量过量存在时，产生带正电的纳米颗粒。相反，当S13βCD/Dhvar4电荷比大于2.5时，可获得带负电的纳米颗粒，其尺寸保持至少30天。具有药理活性的异喹啉生物碱的掺入对在Dhvar4上超过35摩尔%的S13βCD存在下形成的纳米颗粒的性质影响最小。包封率分别为99%和72%。工作图和等温滴定量热测量表明，每个S13βCD大环可以结合约13个这些生物碱分子。本研究结果为未来由三种重要生物医学化合物组成的纳米颗粒的生物学验证和应用奠定了基础。

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引用次数: 0

Corrigendum to “Mechanical, physical, water vapor barrier, and functional properties of carboxymethyl cellulose/anthocyanin/TiO2 films for real-time food quality monitoring” [Carbohydrate Polymer Technologies and Applications 11 (2025) 100877] “用于实时食品质量监测的羧甲基纤维素/花青素/TiO2薄膜的机械、物理、水汽屏障和功能特性”的勘误表[碳水化合物聚合物技术与应用11 (2025)100877]

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101043

Pimonpan Kaewprachu , Chalalai Jaisan , Saroat Rawdkuen , Wirongrong Tongdeesoontorn , Thomas Karbowiak , Pascal Degraeve , Passakorn Kingwascharapong , Samart Sai-Ut , Papungkorn Sangsawad

引用次数: 0

Charge density-driven IPN formation and recovery in hyaluronic acid/κ-carrageenan hydrogels: Novel insights from echo-DWS microrheology, bulk rheology and FTIR 透明质酸/κ-卡拉胶水凝胶中电荷密度驱动的IPN形成和恢复：来自echo-DWS微流变学、体流变学和FTIR的新见解

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101042

Foluso Akin-Ige , Cindy Rivera , Valentina de Gennaro , Yael Faroud Rivera , Samiul Amin

This study investigates how ion valency and coordination behavior modulate the formation, viscoelasticity, and recovery of hyaluronic acid (HA)/

κ

-carrageenan (

κ

CG) hydrogels. Hydrogels were prepared by combining HA and

κ

CG in the presence of monovalent (K

^{+}

), divalent (Ca^2＋), and trivalent (Al^3＋) ions at concentrations of 15–60 mM. Microrheology, Fourier-transform infrared (FTIR) spectroscopy, and bulk oscillatory rheology were employed to correlate molecular interactions with mechanical behavior.

Frequency sweep measurements revealed that the moduli of the hyaluronic acid/

κ

-carrageenan (HA–

κ

CG) blend increased by nearly two orders of magnitude relative to HA alone, confirming synergistic network reinforcement. Among ion-enriched systems, Ca^2＋ produced the highest

G_{plateau}^{'}

(

\sim

20 kPa) and lowest modulus loss between 25

° C

and 37

° C

(

< 10 %

), while Al^3＋ induced intermediate moduli. Recovery tests demonstrated that Ca^2＋-enriched hydrogels recovered

\sim

55% of their initial elasticity within 15 min, compared to

\sim

30% for K

^{+}

and

\sim

40% for Al^3＋ systems. Microrheology confirmed corresponding trends in probe confinement, with subdiffusive exponents (

α

) ranging from 0.18 (K

^{+}

) to 0.32 (Ca^2＋).

FTIR analysis revealed that while K

^{+}

primarily stabilized

κ

CG helices, Ca^2＋ reinforced sulfate junction zones, and Al^3＋ introduced additional HA–carboxylate coordination. Collectively, these results demonstrate that both ion charge and concentration govern the balance between elasticity and deformability in HA–

κ

CG systems, defining whether true interpenetrating or semi-interpenetrating networks emerge. These insights establish design guidelines for improvement of mechanical performance in chemically unmodified HA-based hydrogels.

本研究探讨了离子价和配位行为如何调节透明质酸(HA)/κ-卡拉胶（κCG）水凝胶的形成、粘弹性和恢复。在15-60 mM的浓度下，将HA和κCG结合在一价（K+）、二价（Ca2+）和三价（Al3+）离子下制备水凝胶。利用微流变学、傅里叶变换红外（FTIR）光谱和体振荡流变学分析分子相互作用与力学行为的关系。频率扫描测量显示，透明质酸/κ-卡拉胶（HA -κCG）共混物的模量比单独的HA增加了近两个数量级，证实了协同网络的增强。在离子富集体系中，Ca2+在25°C和37°C之间产生最高的Gplateau ' （~ 20 kPa）和最低的模量损失（<10%），而Al3+诱导中间模量。恢复测试表明，富含Ca2+的水凝胶在15分钟内恢复了初始弹性的~ 55%，而K+为~ 30%，Al3+为~ 40%。微流变学证实了探针约束的相应趋势，亚扩散指数（α）范围为0.18 （K+）至0.32 （Ca2+）。FTIR分析显示，K+主要稳定了κCG螺旋，Ca2+增强了硫酸盐连接区，Al3+引入了额外的ha -羧酸配位。总的来说，这些结果表明，离子电荷和浓度共同控制着HA -κCG系统中弹性和变形性之间的平衡，决定了是否出现真正的互穿或半互穿网络。这些见解为改进未经化学修饰的ha基水凝胶的机械性能建立了设计指南。

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引用次数: 0

Corrigendum to “From waste to wealth: Advancing sustainability with state-of-the-art progress of cellulose nanocrystals and its composites for biomedical applications: A review” [Carbohydrate Polymer Technologies and Applications 11 (2025) 1–41/100974] “从废物到财富：纤维素纳米晶体及其复合材料在生物医学应用中的最新进展：综述”的勘误表[碳水化合物聚合物技术与应用11 (2025)1-41/100974]

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101049

Collince Omondi Awere , Zeeshan Hyderi , Valentine Chikaodili Anadebe , Pandiyan Muthuramalingam , Hyunsuk Shin , Eunice Wamuyu Githae , Eno E. Ebenso , Arumugam Veera Ravi , Manikandan Ramesh

引用次数: 0

Synthesis of Xanthan Gum/Guar Gum/Halloysite nanotubes pH-sensitive hydrogel nanocomposite for controlled release of Quercetin 黄原胶/瓜尔胶/高岭土纳米管ph敏感水凝胶纳米复合材料的合成及其对槲皮素的控释作用

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101060

Negin Nazifi , Mehrab Pourmadadi , Ali Maleki , Majid Abdouss

Developing efficient and pH-sensitive drug delivery systems can facilitate the expedited and cost-effective treatment of cancer, a leading global cause of mortality. A nanocarrier composed of xanthan gum, guar gum, and halloysite nanotubes was prepared via a double nanoemulsion approach and applied to achieve sustained and pH-responsive quercetin release. This system can deliver quercetin to the required site in a controlled way. The presence of each component (HNTs, GG, XG) and the interactions between them were confirmed through FT-IR spectroscopy results. The crystalline structure and related characteristics for each component and the nanocarrier were determined by studying and analyzing XRD. The FE-SEM images indicated that the nanocomposite possesses a hydrogel morphology along with an average particle size of 193 nm. The nanocomposite, moreover, exhibited excellent stability, possessing a surface charge of +43 mV. The addition of HNTs led to improved entrapment and loading efficacy for the drugs, from 70.50 % to 84.75 % and from 36 % to 45.5 %, in contrast to the system containing no HNT. In addition to that, the addition of the polymers XG and GG enhanced the pH sensitivity for the nanocarrier and the release behavior for quercetin (QC), as in vitro experiment results testified. Furthermore, by the study of various kinetic models, the release kinetics for the drugs were determined. MTT assay revealed the nanocarrier to possess significant cytotoxic activity against the HepG2 cancerous cells. While the in vitro results effectively confirm the therapeutic performance of the nanocarrier, their translation to in vivo conditions, unlike in vitro, where the drug interacts directly with the target cells, is influenced by biological factors such as bioavailability, biodistribution, and route of administration. Overall, considering all the obtained results, the GG/XG/HNTs@QC nanocarrier can act effectively in the tumor tissue environment by responding appropriately to pH.

开发高效和ph敏感的药物输送系统可以促进快速和具有成本效益的癌症治疗，这是全球主要的死亡原因。采用双纳米乳法制备了由黄原胶、瓜尔胶和高岭土纳米管组成的纳米载体，并应用于槲皮素的持续释放和ph响应。该系统可以将槲皮素以可控的方式输送到所需的部位。FT-IR结果证实了各组分（HNTs、GG、XG）的存在及其相互作用。通过XRD研究和分析，确定了各组分和纳米载体的晶体结构及相关特性。FE-SEM图像表明，该纳米复合材料具有水凝胶形态，平均粒径为193 nm。此外，纳米复合材料表现出优异的稳定性，表面电荷为+43 mV。与不含HNT的系统相比，加入HNT可提高药物的包裹率和装载效率，从70.50%提高到84.75%，从36%提高到45.5%。此外，体外实验结果表明，聚合物XG和GG的加入增强了纳米载体的pH敏感性和槲皮素（QC）的释放行为。此外，通过各种动力学模型的研究，确定了药物的释放动力学。MTT实验显示纳米载体对HepG2癌细胞具有显著的细胞毒活性。虽然体外结果有效地证实了纳米载体的治疗性能，但它们在体内的转化与体外药物直接与靶细胞相互作用不同，受到生物利用度、生物分布和给药途径等生物因素的影响。综上所述，GG/XG/HNTs@QC纳米载体通过对pH值的适当响应，能够在肿瘤组织环境中有效发挥作用。

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引用次数: 0

Development of a Thiolated Carboxymethyl tara gum derivative with enhanced mucoadhesive and rheological behavior 具有增强黏附性和流变性质的硫代羧甲基塔拉胶衍生物的研制

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101050

Manuel Pariguana , Liz Gonzalez , Clément de Loubens , Esteban Vargas , Adolfo Marican , Ricardo Castro , Gustavo Cabrera-Barjas , Esteban F. Durán-Lara

The rational design of thiolated polysaccharides offers new opportunities to enhance the performance of gastroretentive drug delivery systems by improving mucoadhesive and rheological properties. In this study, thiolated carboxymethyl tara gum (CMTG-SH) was synthesized and optimized (DSSH ≈ 0.9; 2.88 mmol g^-1 of thiols) as a novel, sustainable biopolymer. Structural characterization (FTIR, ¹HNMR , DSC/TGA, SEM, rheology) confirmed successful thiolation and revealed microstructural and thermal modifications. CMTG-SH showed pH-responsive solubility, increasing from 7 to 12 mg mL^-1 (pH 2–3) to 17–40 mg mL^-1 (pH 5–6). Oscillatory rheology demonstrated predominant elastic behavior, and mixtures with mucin (1:2 w/w) exhibited a 40-fold increase in storage modulus (ΔG′/G′ = 39.4 ± 2.6 Pa at pH 6.8) and a maximum bioadhesive force (Fbio = 12.9), evidencing strong synergistic interactions. Even under acidic conditions (pH 4.0), positive reinforcement persisted (ΔG′ = 24.9 ± 2.1 Pa). These results establish CMTG-SH as a robust and sustainable thiomer with significant translational potential for gastroretentive and mucosal drug delivery applications.

巯基多糖的合理设计为通过改善黏附性和流变性能来提高胃保留性给药系统的性能提供了新的机会。本研究合成并优化了硫代羧甲基塔拉胶（CMTG-SH）（DSSH≈0.9；2.88 mmol g-1的硫醇）作为一种新型的可持续生物聚合物。结构表征（FTIR, 1HNMR, DSC/TGA， SEM，流变学）证实了硫基化的成功，并揭示了微观结构和热修饰。CMTG-SH表现出pH响应性溶解度，从7 ~ 12 mg mL-1 （pH 2 ~ 3）增加到17 ~ 40 mg mL-1 （pH 5 ~ 6）。振荡流变学表现出主要的弹性行为，与粘蛋白（1:2 w/w）的混合物显示出40倍的存储模量增加（ΔG ' /G ' = 39.4±2.6 Pa， pH值为6.8）和最大的生物粘附力（Fbio = 12.9），证明了强大的协同作用。即使在酸性条件下（pH 4.0），正强化仍然存在（ΔG ' = 24.9±2.1 Pa）。这些结果表明，CMTG-SH是一种稳健且可持续的硫聚体，在胃保留和粘膜药物递送应用中具有重要的转化潜力。

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引用次数: 0

Alginate-Based cryogenic Bi-nozzle 3D printed hierarchical porous scaffold for accelerating granulation tissue regeneration in exposed bone wounds 海藻酸盐低温双喷嘴3D打印分级多孔支架加速暴露骨伤口肉芽组织再生

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-01 DOI: 10.1016/j.carpta.2025.101052

Jiheng Xiao , Tianqi Liu , Wentian Wang , Hao Liu , Wei Chang , Yiran Zhang , Xianglin Zhang , Yingze Zhang , Bin Wu , Liming Xiong

The exposed bone wound is defined as a kind of wound with bone denuded of periosteum and the deficiency of full-thickness skin and muscles. The wound have no matrix microenvironment and is prone to infection and, hence, hard to heal. Granulation tissue is significant during wound healing, providing a matrix and resisting infection. 3D printing is adopted to fabricate dermis and epidermis regeneration. However, the porosity of most 3D-printed scaffolds is low, leading to a poor drug-loading capacity. To address these limitations, this study developed an alginate-based hierarchical porous scaffold fabricated via cryogenic bi-nozzle 3D printing, enabling dual-factor spatial delivery of VEGF and LL-37 to simultaneously promote angiogenesis and immunomodulation. The alternating dual nozzle printing allows the scaffolds to carry two biological factors with spatial distribution while maintaining the structure integrity. In vitro studies demonstrated enhanced tube formation and endothelial migration, while in vivo implantation in rabbit exposed bone wounds revealed accelerated granulation tissue regeneration and re-epithelialization. The drug-loaded scaffolds can induce the tube formation of vascular endothelial cells, assist inflammatory cells in resisting infection, and ultimately form a fully functional granulation tissue. These results demonstrate the potential clinical applicability of this immunomodulatory dual-factor scaffold for challenging exposed bone wound repair.

外露骨创面是指骨骨膜脱落，缺乏全层皮肤和肌肉的创面。创面无基质微环境，易感染，难以愈合。肉芽组织在伤口愈合过程中是重要的，提供基质和抵抗感染。采用3D打印技术制造真皮和表皮再生。然而，大多数3d打印支架的孔隙度较低，导致其载药能力较差。为了解决这些限制，本研究开发了一种基于海藻酸盐的分层多孔支架，通过低温双喷嘴3D打印制造，使VEGF和LL-37的双因子空间递送同时促进血管生成和免疫调节。交替的双喷嘴打印使支架在保持结构完整性的同时可以携带两种具有空间分布的生物因子。体外研究表明，试管形成和内皮细胞迁移增强，而在兔暴露的骨伤口体内植入显示加速肉芽组织再生和再上皮化。载药支架可诱导血管内皮细胞成管，辅助炎症细胞抵抗感染，最终形成功能齐全的肉芽组织。这些结果证明了这种免疫调节双因子支架在挑战外露骨伤口修复中的潜在临床适用性。

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引用次数: 0

Composite PCL-chitosan scaffolds with dual porosity fabricated via additive manufacturing technique for tissue engineering and regenerative purposes 利用增材制造技术制备具有双重孔隙度的聚氯乙烯-壳聚糖复合支架，用于组织工程和再生

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-11-30 DOI: 10.1016/j.carpta.2025.101056

Ghazal Ahmadian , Maryam Tamimi , Morteza Behzadnasab , Mojgan Zandi , Mohamad Pezeshki-Modaress

During advances in tissue engineering, the morphological aspects of scaffolds, involving pore size and porosity, have been of great importance. Despite all improvements, the challenging issue associated with the constraints of manipulating scaffold architecture using typical fabrication techniques has remained. Regarding this issue, fused deposition modeling, combined with porogen leaching, is a promising approach to enhance pore size and porosity. Using combinatorial methods, we aimed to design composite scaffolds with dual porosity using PCL as a base polymer. Results revealed that the dual pore created after leaching could increase the porosity of constructs, especially PP20CS50W (80.1 ± 0.1), which showed a positive effect on the cellular behavior, as validated by the MTS test and SEM. The combination of PCL with other components could also improve the hydrophilicity of the construct. Furthermore, in vivo investigation revealed that PP20CS50W exhibited higher angiogenesis and fibrotic thickness compared to the others, possibly due to its higher porosity and a successful graft-host response, respectively. It can be concluded that the composite with dual porosity is preferred over the widely used PCL for tissue engineering studies. In addition, this combined methodology, accompanied by dual porosity, may hold promise for future improvements in tissue engineering and regenerative medicine.

在组织工程的进展中，支架的形态学方面，包括孔径和孔隙率，已经变得非常重要。尽管有了这些改进，但与使用典型制造技术操纵支架结构的限制相关的挑战性问题仍然存在。针对这一问题，熔融沉积建模与孔隙浸出相结合是一种很有前景的提高孔隙大小和孔隙度的方法。采用组合方法，设计了以PCL为基材的双孔复合支架。结果表明，浸出后形成的双孔可以增加构建物的孔隙率，特别是PP20CS50W(80.1±0.1)，对细胞行为有积极影响，MTS测试和SEM验证了这一点。PCL与其他组分的结合也能提高构建物的亲水性。此外，体内研究显示，PP20CS50W比其他材料表现出更高的血管生成和纤维化厚度，这可能是由于其更高的孔隙率和成功的移植物-宿主反应。因此，双孔复合材料在组织工程研究中优于广泛使用的聚氯乙烯。此外，这种结合的方法，伴随着双重孔隙度，可能为未来组织工程和再生医学的改进带来希望。

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引用次数: 0

Formulation and statistical optimization of Salvia hispanica L. Seed Polysaccharide/ chitosan nanoparticles for targeted delivery of quercetin in osteosarcoma 鼠尾草种子多糖/壳聚糖纳米颗粒靶向递送槲皮素骨肉瘤的配方及统计优化

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-11-29 DOI: 10.1016/j.carpta.2025.101059

Sara Hasan , Nazrul Islam , Derek Richard , Tony Wang , Emad. L. Izake

Achieving controlled drug release within the tumour microenvironment remains a major barrier to effective chemotherapy. The present study demonstrates the development of a low-cost, biodegradable nanocarrier that responds selectively to acidic tumour conditions, providing a safer alternative to current delivery systems. The hybrid nanoparticles were developed by the self-assembly of chitosan and Salvia hispanica L. seed polysaccharide, which combines the biocompatibility of natural polymers with intrinsic pH sensitivity. A bioactive flavonoid, quercetin, was successfully encapsulated within chitosan and Salvia hispanica L. seed polysaccharide nanoparticles with an efficiency of 78.8 %. The fabricated nanoparticles had a size of 212 nm, an optimal size for passive tumour accumulation through the enhanced permeation and retention effect. A pH-responsive behaviour was observed, with significantly higher release calculated at pH 5.5 (acidic tumor microenvironment) compared to physiological pH 7.4. The kinetic modelling of release profile identified anomalous diffusion as the dominant mechanism under acidic conditions, while fickian diffusion was observed at physiological pH. The cell inhibition potential of quercetin loaded nanoparticles was assessed in U2OS osteosarcoma model. The cell viability assay confirmed that quercetin loaded nanoparticles retained potent metabolic inhibition, indicative of preserved bioactivity of quercetin post-encapsulation.

在肿瘤微环境中控制药物释放仍然是有效化疗的主要障碍。目前的研究展示了一种低成本、可生物降解的纳米载体的发展，这种纳米载体对酸性肿瘤条件有选择性的反应，为目前的输送系统提供了一种更安全的替代方案。将壳聚糖与鼠尾草种子多糖自组装而成的杂化纳米粒子，结合了天然聚合物的生物相容性和固有的pH敏感性。将槲皮素包被壳聚糖和丹参种子多糖纳米颗粒，包被率为78.8%。制备的纳米颗粒的尺寸为212纳米，通过增强的渗透和滞留效应，这是被动肿瘤积累的最佳尺寸。观察到pH响应行为，与生理pH 7.4相比，pH 5.5（酸性肿瘤微环境）计算的释放量显着更高。在酸性条件下，槲皮素的主要释放机制是异常扩散，而在生理ph下，槲皮素的主要释放机制是动态扩散。在U2OS骨肉瘤模型中，槲皮素纳米颗粒的细胞抑制潜力被评估。细胞活力实验证实，负载槲皮素的纳米颗粒保留了有效的代谢抑制作用，表明槲皮素包封后的生物活性得到了保留。

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引用次数: 0