Carbohydrate Polymer Technologies and Applications最新文献_第3页

Chitosan-ZnS nanocomposite hydrogel beads to combat multi-drug-resistant pathogens 壳聚糖-锌纳米复合水凝胶珠对抗多重耐药病原体

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-25 DOI: 10.1016/j.carpta.2025.101078

Rajashree Sahoo , Monalisha Padhiary , Chandra Sekhar Tripathy , Kanhu Charan Behera , Subrata Senapati , Santosh Kumar Behera , Naresh Chandra Bal , Sanghamitra Pati , Sangram Keshari Samal

The extensive use/misuse of antibiotics is a key factor for the rapid rise and global spread of multidrug-resistant (MDR) pathogens, which now pose a major threat to public health. This situation necessitates the development of innovative, biocompatible, and sustainable antimicrobial materials capable of overcoming the limitations of traditional drug-based therapies. Chitosan (CS), a cationic polymer, is an excellent candidate for various medical applications; however, its antimicrobial activity alone may be insufficient against broad-spectrum pathogens. Therefore, in this study, CS-ZnS nanocomposite hydrogel beads were successfully synthesized that demonstrated excellent antibacterial, anti-biofilm properties, as well as suppression of metabolic activity against both Gram-positive and Gram-negative MDR bacterial pathogens. In addition, the hydrogel beads showed significant antioxidant property and demonstrated hemocompatibility. The morphology of this composite was characterized using SEM and TEM, revealing Zinc sulfide (ZnS) nanoflakes sized 8 to 10 nm. The structural properties were evaluated using XRD, Raman, and FTIR spectroscopy. The findings of this study were further supported by in silico analysis, which demonstrated strong interactions with target proteins, including MecA in Staphylococcus aureus and oxacillin-hydrolyzing class D β-lactamase in Pseudomonas aeruginosa. This CS-ZnS system represents a novel, sustainable, and cost-effective biotherapeutic material with potential applications in combating MDR pathogens.

抗生素的广泛使用/误用是耐多药病原体迅速上升和全球传播的一个关键因素，现已对公共卫生构成重大威胁。这种情况需要开发创新的、生物相容性的和可持续的抗菌材料，能够克服传统药物治疗的局限性。壳聚糖（CS）是一种阳离子聚合物，是各种医疗应用的优秀候选者；然而，其单独的抗菌活性可能不足以对抗广谱病原体。因此，在本研究中，我们成功合成了CS-ZnS纳米复合水凝胶珠，它具有优异的抗菌、抗生物膜性能，并能抑制革兰氏阳性和革兰氏阴性MDR细菌病原体的代谢活性。此外，水凝胶珠具有显著的抗氧化性能和血液相容性。利用扫描电镜和透射电镜对复合材料的形貌进行了表征，得到了8 ~ 10 nm的硫化锌纳米片。利用XRD、拉曼光谱和FTIR光谱对其结构性能进行了评价。本研究的结果进一步得到了硅分析的支持，硅分析显示与靶蛋白有很强的相互作用，包括金黄色葡萄球菌中的MecA和铜绿假单胞菌中的oxacillin水解D类β-内酰胺酶。这种CS-ZnS系统代表了一种新型的、可持续的、具有成本效益的生物治疗材料，在对抗耐多药病原体方面具有潜在的应用前景。

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引用次数: 0

Chitosan-based structures for targeting bacterial biofilm-associated genes 靶向细菌生物膜相关基因的壳聚糖结构

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-24 DOI: 10.1016/j.carpta.2025.101077

Shima Afrasiabi

Biofilms are a common lifestyle, in which bacteria grow as multicellular communities. The negative consequences of biofilms are well known. The targeted suppression of certain genes is a promising approach for combating biofilms. Chitosan (CS) has become a focus of biomedical research due to its antimicrobial activity and high biocompatibility. The modification of CS is an important aspect of CS research. The present review deals with the use of CS-based structures in combating bacterial biofilms, with a focus on biofilm-associated genes. CS or CS nanoparticles (NPs), CS functionally modified with natural compounds, metals or metal NPs, or as a drug delivery system are discussed. This review also presents various strategies to improve the performance of CS in biomedical applications such as drug delivery systems, wound healing, bone scaffolds, and dentistry. This knowledge collectively demonstrates the versatility of CS-based structures in disrupting biofilm formation by targeting biofilm-associated genes and provides valuable insights for the development of next-generation antibiofilm therapeutics.

生物膜是一种常见的生活方式，细菌在其中以多细胞群落的形式生长。生物膜的负面影响是众所周知的。靶向抑制某些基因是对抗生物膜的一种很有前途的方法。壳聚糖（CS）因其具有抗菌活性和高生物相容性而成为生物医学研究的热点。CS的修正是CS研究的一个重要方面。本文综述了基于cs的结构在对抗细菌生物膜中的应用，重点介绍了生物膜相关基因。讨论了CS或CS纳米颗粒（NPs）， CS与天然化合物，金属或金属NPs进行功能修饰，或作为药物递送系统。本文还介绍了提高CS在生物医学应用中的性能的各种策略，如药物输送系统、伤口愈合、骨支架和牙科。这些知识共同证明了基于cs的结构在通过靶向生物膜相关基因破坏生物膜形成方面的多功能性，并为开发下一代抗生物膜疗法提供了有价值的见解。

引用次数: 0

Chitooligosaccharides promote intestinal adhesion of Clostridium butyricum and enhance gut health, immunity, and antioxidant capacity in largemouth bass 壳寡糖促进丁酸梭菌肠道黏附，增强大口黑鲈肠道健康、免疫力和抗氧化能力

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-22 DOI: 10.1016/j.carpta.2025.101076

Ge Bai , Xiaohu Li , Honghao Ding , Diying Li , Keke Li , Wen Zhou , Yunxiang Liang , Yingjun Li

Prebiotic polysaccharides and oligosaccharides serve as critical modulators of probiotic functionality, influencing gut microbial ecology and host physiological responses. This study aimed to identify a high-efficacy prebiotic for enhancing the proliferation and intestinal adhesion of Clostridium butyricum (CB) among multiple polysaccharides and to evaluate its impact of combined treatment with CB in largemouth bass (Micropterus salmoides). Through systematic screening, chitooligosaccharides (COS) were selected due to their superior ability to promote CB growth and adhesion. Although no significant differences in growth performance were observed among groups, dietary supplementation with COS and CB in combination reduced hepatic stress markers (ALT: –75.8 %; AST: –83.8 %) and significantly improved intestinal antioxidant capacity (GSH-Px: +282.4 %; T-AOC: +121.0 %; MDA: –34.3 %). RT-qRCR analysis revealed profound upregulation of tight junction proteins (Claudin-1, Claudin-4, ZO-1), indicating enhanced barrier integrity. Hepatic cytokine profiling showed strong immunomodulatory effects, with suppression of IL-1β and IL-8 by 75.7 % and 73.6 %, respectively, and upregulation of IL-10 (2.29-fold) and TCR-α/β (3–4-fold). Gut microbiome restructuring featured a reduction in Pseudomonadota and enrichment of beneficial Bacillota. These results demonstrate that combined COS and CB supplementation improved intestinal barrier function, antioxidant capacity, and immunomodulation, and modulated gut microbiota, suggesting its potential as a sustainable strategy for aquaculture.

益生元多糖和寡糖是益生菌功能的重要调节剂，影响肠道微生物生态和宿主生理反应。本研究旨在寻找一种能在多种多糖中促进丁酸梭菌（Clostridium butyricum， CB）增殖和肠道粘附的高效益生元，并评价其与丁酸梭菌联合处理对大黑鲈（Micropterus salmoides）的影响。通过系统筛选，选择了壳寡糖（COS），因为它具有促进CB生长和粘附的能力。虽然各组间生长性能无显著差异，但饲粮中添加COS和CB可降低肝脏应激指标（ALT: - 75.8%; AST: - 83.8%），显著提高肠道抗氧化能力（GSH-Px: + 282.4%; T-AOC: + 121.0%; MDA: - 34.3%）。RT-qRCR分析显示紧密连接蛋白（Claudin-1, Claudin-4, ZO-1）的深度上调，表明屏障完整性增强。肝脏细胞因子谱显示出较强的免疫调节作用，IL-1β和IL-8分别被抑制75.7%和73.6%,IL-10（2.29倍）和TCR-α/β（3 - 4倍）上调。肠道微生物组重组的特点是假单胞菌减少和有益杆菌的富集。上述结果表明，COS和CB联合添加可改善肠道屏障功能、抗氧化能力和免疫调节能力，并可调节肠道微生物群，表明其在水产养殖中具有可持续发展的潜力。

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引用次数: 0

A novel pH-responsive corn starch/polylactic acid bilayer film: Application for the freshness preservation of hairtail (Trichiurus haumela) 新型ph响应玉米淀粉/聚乳酸双层膜在带鱼保鲜中的应用

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-20 DOI: 10.1016/j.carpta.2025.101075

Haoyun Deng , Xiaoxiao Guo , Chang Sun , Yan Zheng , Mengshi Chen , Bin Zhang , Shanggui Deng , Jiasheng Chen , Jing Chen

Food packaging is essential for preventing contamination, but traditional plastics contribute significantly to pollution, highlighting the need for sustainable alternatives. Intelligent packaging can extend food shelf life by monitoring factors like pH, temperature, and humidity. Hairtail (Trichiurus haumela) is highly perishable, necessitating advanced preservation technologies. A bilayer composite film (ZnO-MAE/CS/PLA) was developed using corn starch (CS) and polylactic acid (PLA) via casting. The CS inner layer is hydrophilic, and the PLA outer layer is hydrophobic. Mulberry anthocyanin extract (MAE) and zinc oxide nanoparticles (ZnO NPs) provide antioxidant, antimicrobial, and pH-responsive properties. Unlike petroleum-based dyes, MAE is non-toxic and eco-friendly. SEM analysis showed uniform ZnO NP dispersion at 2–4%, improving tensile strength (8.08 to 9.58 MPa) and elongation (24.71% to 25.56%). Higher concentrations led to aggregation and slight reductions in mechanical properties. Optimal performance, with balanced mechanical strength, antimicrobial activity against Escherichia coli and Staphylococcus aureus, and pH sensitivity, was observed at 6% ZnO NPs. The film also inhibited microbial growth and nutrient oxidation, significantly extending the shelf life of hairtail. Thus, ZnO-MAE/CS/PLA films hold great potential as intelligent, eco-friendly packaging for hairtail preservation.

食品包装对防止污染至关重要，但传统塑料会严重造成污染，因此需要可持续的替代品。智能包装可以通过监测pH值、温度和湿度等因素来延长食品的保质期。带鱼极易腐烂，需要先进的保存技术。以玉米淀粉（CS）和聚乳酸（PLA）为原料，通过浇铸法制备了ZnO-MAE/CS/PLA双层复合薄膜。CS内层为亲水性，PLA外层为疏水性。桑树花青素提取物（MAE）和氧化锌纳米颗粒（ZnO NPs）具有抗氧化、抗菌和ph响应特性。与石油基染料不同，MAE是无毒和环保的。SEM分析表明，ZnO NP分散均匀，在2-4%范围内，提高了抗拉强度（8.08 ~ 9.58 MPa）和伸长率（24.71% ~ 25.56%）。较高的浓度会导致聚集和机械性能的轻微降低。在6% ZnO NPs中，该材料具有平衡的机械强度、对大肠杆菌和金黄色葡萄球菌的抑菌活性和pH敏感性。该膜还能抑制带鱼的微生物生长和营养物质氧化，显著延长带鱼的保质期。因此，ZnO-MAE/CS/PLA薄膜作为带鱼保存的智能、环保包装具有很大的潜力。

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引用次数: 0

p-Sulfonated calix[4]arene grafted on magnetic chitosan as efficient and recyclable multifunctional bio-nanocatalyst for various organic transformations 磁性壳聚糖接枝对磺酸杯芳烃作为多种有机转化的高效、可回收多功能生物纳米催化剂

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-19 DOI: 10.1016/j.carpta.2025.101074

Saied Shafiei Navid, Rahman Hosseinzadeh, Mohammad Salehi

Biopolymers are abundant and sustainable materials that have various benefits, including reducing the development of secondary pollution because of their inherent environmental friendliness. For this reason, this study aims to prepare a chitosan-based magnetic nanocatalyst by grafting sulfonated calix[4]arene as a Brønsted acid moiety onto magnetic chitosan using isophorone diisocyanate as a linker. The prepared bio-based nanocatalyst, designated as SCA@MCS, was characterized using various analysis methods, including FT-IR, PXRD, TGA, SEM, EDS/MAPP, TEM, CHNS, VSM, and Zeta potential. The simultaneous presence of chitosan and p-sulfonated calix[4]arene endows SCA@MCS with both acidic and basic active sites, making it an effective multifunctional nanocatalyst. Consequently, the bio-based SCA@MCS was applied as a reusable heterogenous nanocatalyst for the efficient synthesis of various organic compounds, including dihydropyridine (89–98 %), dihydropyrimidine (82–98 %), dihydropyrrolone (89–95 %), and benzoate ester derivatives (98–99 %). The absence of toxic solvents, mild reaction conditions, simple work-up procedures, the use of a low amount of nanocatalyst, high reaction yields, and the reusability of the nanocatalyst are the key advantages of the introduced SCA@MCS magnetic nanocatalyst. These results demonstrate that the grafting of sulfonated calix[4]arene onto magnetic chitosan provides an efficient bio-based multifunctional nanocatalyst for the synthesis of various biologically active compounds and biodiesel production.

生物聚合物是一种丰富的可持续材料，由于其固有的环境友好性，具有减少二次污染等多种效益。为此，本研究旨在以异佛尔酮二异氰酸酯为连接剂，将磺化的杯状芳烃作为br / nsted酸段接枝到磁性壳聚糖上，制备壳聚糖基磁性纳米催化剂。采用FT-IR、PXRD、TGA、SEM、EDS/MAPP、TEM、CHNS、VSM和Zeta电位等多种分析方法对制备的生物基纳米催化剂SCA@MCS进行了表征。壳聚糖和对磺化杯芳烃的同时存在使SCA@MCS具有酸性和碱性活性位点，成为一种有效的多功能纳米催化剂。因此，生物基SCA@MCS被用作可重复使用的多相纳米催化剂，用于高效合成各种有机化合物，包括二氢吡啶（89 - 98%）、二氢嘧啶（82 - 98%）、二氢吡咯酮（89 - 95%）和苯甲酸酯衍生物（98 - 99%）。无有毒溶剂，反应条件温和，后处理程序简单，纳米催化剂用量少，反应收率高，纳米催化剂的可重复使用是SCA@MCS磁性纳米催化剂的主要优点。这些结果表明，在磁性壳聚糖上接枝磺化杯芳烃为合成各种生物活性化合物和生产生物柴油提供了一种高效的生物基多功能纳米催化剂。

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引用次数: 0

A synthetic 6-O-sulfated glycopolymer induces tissue iron accumulation via competitively inhibiting the hepcidin/FPN1 axis 合成的6- o -硫代糖共聚物通过竞争性抑制hepcidin/FPN1轴诱导组织铁积累

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-19 DOI: 10.1016/j.carpta.2025.101073

Zhujie Xu , Yi Liu , Jingxiao Chen , Bangjun Wen , Youjia Xu

Highly sulfated heparin-like glycosaminoglycans (GAGs) have been closely linked to disrupted iron homeostasis, yet no studies have employed site-specific sulfation modifications of GAGs to develop macromolecular compounds capable of modulating iron metabolism. Based on preliminary research, our team designed and synthesized an glycopolymer with 6-O-sulfated GlcNAc. In vitro administration experiments demonstrated that 6-O-sulfated glycopolymer significantly inhibits hepcidin-mediated internalization and degradation of ferroportin 1 (FPN1) on macrophage membranes, reducing cellular iron storage— an effect superior to that of conventional HS. Following CY5-labeled 6-O-sulfated glycopolymer administration in vivo, fluorescence tracking revealed broad tissue distribution, including abdominal organs and cortical bone. Meanwhile, 6-O-sulfated glycopolymer markedly decreased serum hepcidin level, increased serum ferritin concentration, and induced significant iron accumulation in the liver and bone cortex, while results from HE staining of hepatic, splenic, and renal tissues confirmed its biological safety. Computational docking, surface plasmon resonance (SPR) assay, and cellular thermal shift assay-western blot (CETSA-WB) further demonstrated high-affinity binding between 6-O-sulfated glycopolymer and hepcidin. These findings establish the 6-O-sulfate group as the critical functional moiety in HS-mediated systemic iron regulation, where 6-O-sulfated glycopolymer competitively inhibits the hepcidin/FPN1 axis to activate macrophage iron efflux, ultimately promoting tissue iron accumulation.

高硫酸化的肝素样糖胺聚糖（GAGs）与铁稳态的破坏密切相关，但目前还没有研究利用特定位点的硫酸化修饰GAGs来开发能够调节铁代谢的大分子化合物。在前期研究的基础上，我们设计并合成了6- o -硫酸化GlcNAc的糖共聚物。体外给药实验表明，6- o -硫代糖共聚物显著抑制hepcidin介导的巨噬细胞膜上铁转运蛋白1 （FPN1）的内化和降解，减少细胞铁的储存，其效果优于常规HS。在体内给药cy5标记的6- o -硫代糖共聚物后，荧光跟踪显示广泛的组织分布，包括腹部器官和皮质骨。同时，6- o -硫代糖共聚物显著降低血清hepcidin水平，升高血清铁蛋白浓度，诱导肝脏和骨皮质明显的铁积累，肝、脾、肾组织HE染色结果证实其生物安全性。计算对接、表面等离子体共振（SPR）实验和细胞热移测定-western blot （CETSA-WB）进一步证明了6- o -硫酸化糖共聚物与hepcidin之间的高亲和力结合。这些发现证实了6- o -硫酸盐基团是hs介导的系统性铁调节的关键功能片段，其中6- o -硫代糖共聚物竞争性地抑制hepcidin/FPN1轴，激活巨噬细胞铁外排，最终促进组织铁积累。

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引用次数: 0

Charged carboxymethyl chitosan based CO2-philic adsorbents for enhanced CO2 capture and release via frequency using steel mesh electric polarization device at low temperature 利用钢网电极化装置在低温下通过频率增强二氧化碳捕获和释放的带电羧甲基壳聚糖基亲二氧化碳吸附剂

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-18 DOI: 10.1016/j.carpta.2025.101072

Chun-Yao Huang , Feng-Yen Lin , Muhammad Iqbal , Wei-Song Hung , Chien-Hsing Lu , Jem-Kun Chen

Electric polarization (EP) facilitates charge separation within carbohydrate polymers under an electric field—a phenomenon that has not been thoroughly explored for CO₂ capture. To investigate this effect, carboxymethyl chitosan (CMCS)-based molecularly imprinted polymers (MIPs) were applied as a shell layer over polyacrylonitrile fibrous membranes (Pfm), creating a core–shell Pfm/MIP that formed amide-rich, CO₂-attractive binding sites. The Pfm/MIPs were positioned between two conductive mesh electrodes, where an alternating electric field was applied during CO₂ adsorption. Without EP, the CO₂ adsorption of Pfm/MIP was measured at 0.90 mmol/g. However, under an EP frequency of 300 kHz and 16 V, the dynamic adsorption increased markedly to 1.66 mmol/g. At elevated frequencies (e.g., 1200 kHz), the amine dipoles fail to synchronize with the rapid field oscillations, leading to dielectric loss and promoting CO₂ release from the imprinted sites. At a partial pressure of 0.15 bar CO₂, the selectivity for CO₂ over N₂ ranged from 65.5 to 67.4, while the purity of the recovered CO₂ stream was between 92.7% and 93.9%. The Pfm/MIP system demonstrated recyclability across 70 cycles of alternating EP frequencies between 300 and 1200 kHz. This approach highlights the role of charged adsorbents in modulating CO₂ adsorption and desorption behavior.

电场作用下，电极化（EP）促进了碳水化合物聚合物内的电荷分离——这一现象尚未被充分研究用于CO₂捕获。为了研究这种效应，将羧甲基壳聚糖（CMCS）为基础的分子印迹聚合物（MIPs）作为壳层应用于聚丙烯腈纤维膜（Pfm）上，形成了核-壳Pfm/MIP，形成了富含酰胺的、具有CO 2吸引力的结合位点。Pfm/MIPs被放置在两个导电网状电极之间，在CO₂吸附过程中施加交变电场。在不加EP的情况下，Pfm/MIP的CO₂吸附量为0.90 mmol/g。而在EP频率为300 kHz，电压为16 V时，动态吸附显著增加至1.66 mmol/g。在较高的频率下（例如，1200 kHz），胺偶极子不能与快速场振荡同步，导致介电损耗并促进co2从印迹位点释放。在0.15 bar CO₂的分压下，CO₂对N₂的选择性为65.5 ~ 67.4，而回收的CO₂流的纯度为92.7% ~ 93.9%。Pfm/MIP系统在300至1200 kHz的交替EP频率下进行了70次循环，证明了其可回收性。这种方法强调了带电吸附剂在调节CO₂吸附和解吸行为中的作用。

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引用次数: 0

ROS-Responsive Iodine-Embedded Cyclodextrin Nanoplatform for Multipurpose Periodontitis Therapy 多用途牙周炎治疗的ros响应碘包埋环糊精纳米平台

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-17 DOI: 10.1016/j.carpta.2025.101070

Siyu He , Huipeng Xu , Siwen Wang , Xintao Chen , Yunxi Li , Wuzhen Ma , Xiaohong Ren , Li Wu , Yanling Xue , Jiwen Zhang

Periodontitis, an inflammatory disorder driven by microbial dysbiosis, is featured as elevated reactive oxygen species (ROS) and dysregulated inflammation, leading to tissue destruction and alveolar bone resorption. A promising therapeutic approach involves antimicrobial actions with ROS neutralization. Addressing the clinical limitation of elemental iodine (I₂) to topical use, we engineered a ROS-scavenging covalent cyclodextrin framework (CCF) embedding I₂ to confer dual functionality for periodontitis treatment. Initially, potassium iodide cyclodextrin metal-organic framework (KI-CD-MOF) was synthesized, which was then cross-linked with oxalyl chloride to introduce a ROS-scavenging peroxalate ester bond. Simultaneously, the iodide ions in KI-CD-MOF were oxidized to I₂, which was integrated throughout the framework. The resulting I₂-CCF particles had a cubic morphology (300–500 nm) and an I₂ content of 1.03±0.45 %, exhibiting an outstanding biosafety profile, strong bactericidal effectiveness, and significant ROS elimination capability. In vitro, I₂-CCF downregulated the expression of pro-inflammatory factors TNF-α and IL-1β, while promoting the secretion of anti-inflammatory markers IL-10 and Arg-1. Crucially, in a rodent periodontitis model, I₂-CCF effectively suppressed dental plaque accumulation, reduced periodontal inflammation, and limited alveolar bone resorption. This work establishes I₂-CCF as a multipurpose nanotherapeutic that concurrently targets the infectious and oxidative components of periodontitis, offering a potent alternative to conventional treatments.

牙周炎是一种由微生物生态失调引起的炎症性疾病，其特征是活性氧（ROS）升高和炎症失调，导致组织破坏和牙槽骨吸收。一种很有前途的治疗方法包括与ROS中和的抗菌作用。为了解决局部使用元素碘（I2）的临床局限性，我们设计了一种包埋I2的共价环糊精框架（CCF），以赋予治疗牙周炎的双重功能。首先合成碘化钾环糊精金属有机骨架（KI-CD-MOF），然后与草酰氯交联，形成清除ros的过草酸酯键。同时，KI-CD-MOF中的碘离子被氧化为I2， I2在整个框架中被整合。得到的I2- ccf颗粒具有立方形态（300-500 nm）， I2含量为1.03±0.45%，具有良好的生物安全性、强杀菌效果和显著的ROS消除能力。在体外，I2-CCF下调促炎因子TNF-α和IL-1β的表达，促进抗炎标志物IL-10和Arg-1的分泌。关键是，在啮齿动物牙周炎模型中，I2-CCF有效抑制牙菌斑积累，减少牙周炎症，限制牙槽骨吸收。这项工作建立了I2-CCF作为一种多用途的纳米治疗药物，同时针对牙周炎的感染性和氧化性成分，为传统治疗提供了一种有效的替代方案。

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引用次数: 0

Tuning the biological scaffolds’ performance by the combination of two antioxidant and antimicrobial chitosan derivatives 两种抗氧化和抗菌壳聚糖衍生物的组合调节生物支架的性能

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-16 DOI: 10.1016/j.carpta.2025.101069

C. Muñoz-Núñez , A. Barco-Martín , K. Deshpande , D.S. Schmidt , L. González-García , S. Trujillo , A. Muñoz-Bonilla , M. Fernández-García

In this study novel polymeric materials based on chitosan (CS) were synthesized by chemically modifying CS with two bioactive moieties: eugenol and a compound containing a thiazolium group. These modifications aimed to impart antioxidant and antimicrobial properties to the matrix. Additionally, the scaffolds were reinforced with chitin nanowhiskers (Nw) to improve their mechanical strength and stability. Porous three-dimensional scaffolds were fabricated via the freeze-drying process, resulting in highly interconnected pore networks suitable for cell infiltration and nutrient transport. Biological characterization revealed that the incorporation of the two bioactive groups significantly enhanced the antioxidant activity and antimicrobial efficacy against both Gram-positive and Gram-negative bacteria to the scaffolds. Mechanical testing demonstrated that the Nw reinforcement increased scaffold stiffness and resilience without compromising porosity. In vitro biological assays using fibroblasts showed favorable cytocompatibility and promoted sustained cell proliferation over three weeks. Fluorescence microscopy confirmed fibroblast adhesion and morphological adaptation within the scaffold architecture. Additionally, the scaffolds were evaluated for their immunomodulatory effects using macrophage cultures, revealing a balanced immune response with reduced proinflammatory signaling, which is critical for successful integration and reduced fibrosis in vivo. These results indicate that those are promising candidates for tissue engineering and regenerative medicine applications.

在壳聚糖的基础上，用丁香酚和含噻唑基团的化合物对壳聚糖进行化学修饰，合成了新型高分子材料。这些修饰旨在赋予基质抗氧化和抗菌特性。此外，甲壳素纳米晶须（Nw）增强了支架的机械强度和稳定性。通过冷冻干燥工艺制备多孔三维支架，形成适合细胞浸润和营养物质运输的高度互联的孔隙网络。生物学特性表明，两种生物活性基团的掺入显著增强了支架对革兰氏阳性菌和革兰氏阴性菌的抗氧化活性和抗菌活性。力学测试表明，Nw加固在不影响孔隙率的情况下增加了支架的刚度和弹性。使用成纤维细胞进行的体外生物试验显示出良好的细胞相容性，并在三周内促进持续的细胞增殖。荧光显微镜证实了成纤维细胞的粘附和支架结构的形态适应。此外，使用巨噬细胞培养评估了支架的免疫调节作用，揭示了平衡的免疫反应，减少了促炎信号，这对于成功整合和减少体内纤维化至关重要。这些结果表明，这些是有希望的候选组织工程和再生医学应用。

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引用次数: 0

Valorizing date waste into functional bacterial nanocellulose with dual adsorption–antifouling properties for wastewater remediation 将枣泥废渣转化为具有双重吸附-防污性能的功能性细菌纳米纤维素用于废水修复

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-12-16 DOI: 10.1016/j.carpta.2025.101071

Jahirul Ahmed Mazumder , Aniss Kedjour , Adiba Akram , Mussarat Tasleem , Fawzi Banat

The valorization of agro-industrial byproducts is crucial for establishing circular bioeconomies. This study presents a novel approach to produce bacterial nanocellulose (BNC) using date seeds (DS) and pomace (DP) as sustainable, low-cost carbon feedstocks for Komagataeibacter hansenii and examines whether feedstock chemistry alone can program the functionality of BNC. DP and DS extracts presented contrasting soluble profiles, which translated into differences in BNC yield and function. DP-BNC showed superior production and hydration (WUC = 552 ± 31 % vs 480 ± 25 % for DS-BNC) and retained the cellulose I structure. Spectroscopic studies indicated the in situ incorporation of hydrophilic motifs, and ζ-potential measurements at pH 7 revealed a negative surface charge (∼ −25.6 mV) of BNC. Furthermore, DP-BNC functioned as a highly effective biosorbent, achieving adsorption capacities of 4.62 ± 0.26 mg/g for the cationic dye Crystal Violet (CV) and 3.85 ± 0.20 mg/g for Methyl Orange (MO), with pseudo-second-order kinetics and Langmuir equilibria; capacities were retained at ∼52 % for CV and ∼48 % for MO till the fifth cycle. Antifouling assays demonstrated 41.4 % lower BSA adsorption and 93.1 % lower Escherichia coli colonization on DP-BNC compared to DS-BNC. This research establishes a pragmatic pathway for transforming low-value dates residues into a high-performance biomaterial.

农业工业副产品的增值对建立循环生物经济至关重要。本研究提出了一种利用枣籽（DS）和渣（DP）作为Komagataeibacter hansenii的可持续、低成本碳原料生产细菌纳米纤维素（BNC）的新方法，并研究了原料化学是否可以单独编程BNC的功能。DP和DS提取物具有不同的可溶性谱，这导致了BNC产量和功能的差异。DP-BNC表现出较好的产量和水合作用（WUC = 552±31%，而DS-BNC为480±25%），并保留了纤维素I的结构。光谱研究表明亲水性基序的原位结合，在pH 7下的ζ电位测量显示BNC的表面带负电荷（~ - 25.6 mV）。此外，DP-BNC作为一种高效的生物吸附剂，对阳离子染料结晶紫（CV）的吸附量为4.62±0.26 mg/g，对甲基橙（MO）的吸附量为3.85±0.20 mg/g，具有准二级动力学和Langmuir平衡；CV和MO的容量分别保持在~ 52%和~ 48%，直到第五个循环。防污实验表明，与DS-BNC相比，DP-BNC的BSA吸附量降低41.4%，大肠杆菌定植量降低93.1%。本研究建立了将低价值枣渣转化为高性能生物材料的实用途径。

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