Carbohydrate Polymer Technologies and Applications最新文献_第3页

Amine modified sodium alginate: Synthesis, characterization and in vivo evaluation in rainbow trout (Oncorhynchus mykiss)

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-06 DOI: 10.1016/j.carpta.2025.100699

Samira Salarvand , Seyed Amir Hossein Jalali , Nasrollah Mahboobi Soofiani , Alireza Allafchian

Alginate is a natural linear anionic biopolymer abundantly found in the seabed. Alginate becomes an adequate option as a diet improver or drug/gene carrier through chemical modification processes and changes in its structure. This study modified a simple sodium alginate by oxidation and reductive amination processes. The characteristics of modified sodium alginate were evaluated by FTIR, XRD, zeta potential, FE-SEM, EDX, and MAP analysis. Its cytotoxicity was evaluated using an MTT assay. Finally, its effects were assessed orally in rainbow trout (3 ± 0.3 g) for 6 weeks at 2 levels (2.5 and 5 g kg^-1). The characterization showed that the chemical modification process was successful, and amine groups were incorporated into the alginate structure. The amount of amine added to modified sodium alginate was 2.53 % based on EDX. MTT results showed no cytotoxicity for modified alginate. The in vivo results showed that amine-modified alginate treatments significantly increased non-specific immune parameters, including lysozyme activity, alternative complement activity, and serum bactericidal activity, and also enhanced intestinal bacterial population compared with the control and simple alginate treatments (P < 0.05). Administration of 2.5 and 5 g kg^-1 modified alginate significantly increased FCR (0.84±0.01 and 0.83±0.04, respectively) and other growth parameters compared with other experimental groups (P < 0.05). Also, after being challenged by ozone, amine-modified alginate fish groups showed the highest relative percent survival (RPS) values (75 %, 90 %). This improvement can be attributed to the modified alginate's nature and the amine groups' role in its structure. Therefore, amine-modified alginate can be suggested as an immunostimulant.

{"title":"Amine modified sodium alginate: Synthesis, characterization and in vivo evaluation in rainbow trout (Oncorhynchus mykiss)","authors":"Samira Salarvand , Seyed Amir Hossein Jalali , Nasrollah Mahboobi Soofiani , Alireza Allafchian","doi":"10.1016/j.carpta.2025.100699","DOIUrl":"10.1016/j.carpta.2025.100699","url":null,"abstract":"<div><div>Alginate is a natural linear anionic biopolymer abundantly found in the seabed. Alginate becomes an adequate option as a diet improver or drug/gene carrier through chemical modification processes and changes in its structure. This study modified a simple sodium alginate by oxidation and reductive amination processes. The characteristics of modified sodium alginate were evaluated by FTIR, XRD, zeta potential, FE-SEM, EDX, and MAP analysis. Its cytotoxicity was evaluated using an MTT assay. Finally, its effects were assessed orally in rainbow trout (3 ± 0.3 g) for 6 weeks at 2 levels (2.5 and 5 g kg<sup>-1</sup>). The characterization showed that the chemical modification process was successful, and amine groups were incorporated into the alginate structure. The amount of amine added to modified sodium alginate was 2.53 % based on EDX. MTT results showed no cytotoxicity for modified alginate. The <em>in vivo</em> results showed that amine-modified alginate treatments significantly increased non-specific immune parameters, including lysozyme activity, alternative complement activity, and serum bactericidal activity, and also enhanced intestinal bacterial population compared with the control and simple alginate treatments (<em>P</em> < 0.05). Administration of 2.5 and 5 g kg<sup>-1</sup> modified alginate significantly increased FCR (0.84±0.01 and 0.83±0.04, respectively) and other growth parameters compared with other experimental groups (<em>P</em> < 0.05). Also, after being challenged by ozone, amine-modified alginate fish groups showed the highest relative percent survival (RPS) values (75 %, 90 %). This improvement can be attributed to the modified alginate's nature and the amine groups' role in its structure. Therefore, amine-modified alginate can be suggested as an immunostimulant.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100699"},"PeriodicalIF":6.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143351141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yogurt enrichment with sulfated alginate microcapsules containing garlic- and cardamom essential oils-loaded β-cyclodextrin: Investigating physicochemical and biological properties

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-05 DOI: 10.1016/j.carpta.2025.100694

Marjan Teymuri-Yeghaneh , Davoud Salarbashi , Hossein Derakhshankhah , Mehdi Jaymand

Side effects of synthetic food additives caused considerable concerns. In this context, natural substances such as essential oils (EOs) have received significant interest owing to their safety as well as anti-oxidant, anti-microbial, anti-cancer, anti-inflammatory, hypoglycemic, and some other biological properties. The purpose of this study was to use natural preservatives to determine the effect of sulfated alginate (S-Alg) microcapsules contaning cardamom (CEO)- and garlic (GEO) essential oils-loaded β-cyclodextrin (β-CD) on pathogenic and spoilage bacteria as well as anti-oxidant properties to increase shelf-life in yogurt. For this purpose, CEO and GEO were loaded into β-CD, and then encapsulated into S-Alg microparticles. The microcapsules containing CEO- and GEO-loaded β-CD were added to milk at three different concentrations (0.5, 1, and 1.5 % w/v) to prepare yogurt, and then anti-oxidant, pH, syneresis, sensory, and anti-bacterial properties of the resultant yogurt were evaluated during 21 days of storage. It was found that adding encapsulated CEO and GEO increased the shelf-life of yogurt and preserved its sensory and anti-bacterial properties during 21 days of storage compared to pure EOs.

{"title":"Yogurt enrichment with sulfated alginate microcapsules containing garlic- and cardamom essential oils-loaded β-cyclodextrin: Investigating physicochemical and biological properties","authors":"Marjan Teymuri-Yeghaneh , Davoud Salarbashi , Hossein Derakhshankhah , Mehdi Jaymand","doi":"10.1016/j.carpta.2025.100694","DOIUrl":"10.1016/j.carpta.2025.100694","url":null,"abstract":"<div><div>Side effects of synthetic food additives caused considerable concerns. In this context, natural substances such as essential oils (EOs) have received significant interest owing to their safety as well as anti-oxidant, anti-microbial, anti-cancer, anti-inflammatory, hypoglycemic, and some other biological properties. The purpose of this study was to use natural preservatives to determine the effect of sulfated alginate (S-Alg) microcapsules contaning cardamom (CEO)- and garlic (GEO) essential oils-loaded β-cyclodextrin (β-CD) on pathogenic and spoilage bacteria as well as anti-oxidant properties to increase shelf-life in yogurt. For this purpose, CEO and GEO were loaded into β-CD, and then encapsulated into S-Alg microparticles. The microcapsules containing CEO- and GEO-loaded β-CD were added to milk at three different concentrations (0.5, 1, and 1.5 % w/v) to prepare yogurt, and then anti-oxidant, pH, syneresis, sensory, and anti-bacterial properties of the resultant yogurt were evaluated during 21 days of storage. It was found that adding encapsulated CEO and GEO increased the shelf-life of yogurt and preserved its sensory and anti-bacterial properties during 21 days of storage compared to pure EOs.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100694"},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chito-oligosaccharides (COS) with anti-aging effect from Flammulina velutipes

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-05 DOI: 10.1016/j.carpta.2025.100705

Qian Teng, Peng Wang, Fei Fang, Qinghong Liu

Chito-oligosaccharides (COS) with a degree of deacetylation (DD) of 67.85 % and a degree of polymerization (DP) ranging from 2 to 6 was prepared from Flammulina velutipes (F. velutipes). The COS was confirmed as α-form by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Nuclear magnetic resonance analysis (NMR). Furthermore, the in vitro antioxidant activity of COS was evaluated, along with its anti-aging effects in D-Galactose (D-gal)-induced aging BALB/c mice. COS treatment demonstrated a dose-dependent enhancement in the scavenging activities of DPPH, ABTS, and hydroxyl radicals. Additionally, COS treatment alleviated tissue injury in the liver and kidney of mice induced by D-gal. The antioxidative indices, including superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px), were improved in the liver of mice treated with COS. Moreover, the relative expressions of inflammatory factors Interleukin-1β (IL-1β) and Interleukin-6 (IL-6) were reduced in the COS treatment groups. These findings indicate that F. velutipes COS exhibits resistance to D-gal-induced aging, with mechanisms linked to its antioxidant and immunoregulatory activities. This suggests that F. velutipes COS may serve as a potential source of antioxidant and anti-aging agent.

{"title":"Chito-oligosaccharides (COS) with anti-aging effect from Flammulina velutipes","authors":"Qian Teng, Peng Wang, Fei Fang, Qinghong Liu","doi":"10.1016/j.carpta.2025.100705","DOIUrl":"10.1016/j.carpta.2025.100705","url":null,"abstract":"<div><div>Chito-oligosaccharides (COS) with a degree of deacetylation (DD) of 67.85 % and a degree of polymerization (DP) ranging from 2 to 6 was prepared from <em>Flammulina velutipes</em> (<em>F. velutipes</em>). The COS was confirmed as α-form by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Nuclear magnetic resonance analysis (NMR). Furthermore, the <em>in vitro</em> antioxidant activity of COS was evaluated, along with its anti-aging effects in D-Galactose (D-gal)-induced aging BALB/c mice. COS treatment demonstrated a dose-dependent enhancement in the scavenging activities of DPPH, ABTS, and hydroxyl radicals. Additionally, COS treatment alleviated tissue injury in the liver and kidney of mice induced by D-gal. The antioxidative indices, including superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px), were improved in the liver of mice treated with COS. Moreover, the relative expressions of inflammatory factors Interleukin-1β (IL-1β) and Interleukin-6 (IL-6) were reduced in the COS treatment groups. These findings indicate that <em>F. velutipes</em> COS exhibits resistance to D-gal-induced aging, with mechanisms linked to its antioxidant and immunoregulatory activities. This suggests that <em>F. velutipes</em> COS may serve as a potential source of antioxidant and anti-aging agent.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100705"},"PeriodicalIF":6.2,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-assisted sedimentation analysis of cellulose nanofibers to predict the specific surface area

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-04 DOI: 10.1016/j.carpta.2025.100697

Koyuru Nakayama, Akio Kumagai, Keita Sakakibara

This study introduced a novel machine learning (ML) approach for predicting the specific surface area (SSA) of cellulose nanofibers (CNFs) at various fibrillation stages by leveraging sedimentation profiles from their aqueous slurries. Both sedimentation speed and sedimentation heatmap images, derived from the sedimentation profile data, formed the basis of the ML-assisted prediction model, achieving a coefficient of determination (R²) of up to 0.94 for SSA prediction. The high R² values can be obtained through the appropriate ML algorithms used for the prediction model, including extreme gradient-boosting (XGBoost) regression and convolutional neural networks (CNN) for sedimentation speed and sedimentation heatmap images, respectively, which are effective to deal with these sedimentation data, enabling accurate predictions. Furthermore, the predicted SSA values were used for the construction of the prediction model for impact strength of polypropylene/ wood-derived CNF composite materials by integrating with the infrared spectrum data of the CNFs, achieving the improved R² of 0.88, as compared to the conventional models based on experimentally obtained SSA with R² = 0.79. This sedimentation analysis method therefore enables the acquisition of information related to the morphology of CNFs, which can be widely applied in the quality control of CNFs as well as in the material applications.

{"title":"Machine learning-assisted sedimentation analysis of cellulose nanofibers to predict the specific surface area","authors":"Koyuru Nakayama, Akio Kumagai, Keita Sakakibara","doi":"10.1016/j.carpta.2025.100697","DOIUrl":"10.1016/j.carpta.2025.100697","url":null,"abstract":"<div><div>This study introduced a novel machine learning (ML) approach for predicting the specific surface area (SSA) of cellulose nanofibers (CNFs) at various fibrillation stages by leveraging sedimentation profiles from their aqueous slurries. Both sedimentation speed and sedimentation heatmap images, derived from the sedimentation profile data, formed the basis of the ML-assisted prediction model, achieving a coefficient of determination (R²) of up to 0.94 for SSA prediction. The high R<sup>2</sup> values can be obtained through the appropriate ML algorithms used for the prediction model, including extreme gradient-boosting (XGBoost) regression and convolutional neural networks (CNN) for sedimentation speed and sedimentation heatmap images, respectively, which are effective to deal with these sedimentation data, enabling accurate predictions. Furthermore, the predicted SSA values were used for the construction of the prediction model for impact strength of polypropylene/ wood-derived CNF composite materials by integrating with the infrared spectrum data of the CNFs, achieving the improved R² of 0.88, as compared to the conventional models based on experimentally obtained SSA with R<sup>2</sup> = 0.79. This sedimentation analysis method therefore enables the acquisition of information related to the morphology of CNFs, which can be widely applied in the quality control of CNFs as well as in the material applications.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100697"},"PeriodicalIF":6.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conductive supramolecular acrylate hydrogels enabled by quaternized chitosan ionic crosslinking for high-fidelity 3D printing

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-04 DOI: 10.1016/j.carpta.2025.100702

Lukas A. Bauman, Boxin Zhao

While 3D printing has enabled the fabrication of hydrogels with complex structures, high fidelity techniques (vat polymerization) that enable precisely engineered design of hydrogels require stiff structures to withstand the forces of printing. This is a pressing research gap in hydrogel vat-polymerization 3D printing. To address this limitation, a novel ionic crosslinker consisting of quaternized chitosan complexed with 3-sulfopropyl acrylate was used to form supramolecular 2-hydroxyethyl acrylate organogel precursors. The Cyrene organogel enhanced mechanical properties enabling the printing of high-fidelity structures; the final compliant hydrogels were then obtained through solvent exchange with water. This yielded high-fidelity 3D-printed conductive supramolecular hydrogels with tensile properties of 288±29 kPa at 516±37 % elongation and compressive properties of 572±34 kPa at 65±4 % strain with uniform swelling (320–350 %). Nuclear magnetic resonance and conductivity measurements confirmed SPA-rich blocks within the hydrogel network and the solvent-dependent copolymer structure. Furthermore, through varying the anionic acrylate concentration, ultimate strain between 222 % and 516 % was achieved at a constant elastic modulus. Additionally, electrical properties were tunable with conductivity reaching 156 mS/m at 7 MH in ultrapure water. This work advances applications of quaternized chitosan as an ionic crosslinker in printable conductive hydrogels, opening new applications in medical and technological fields.

{"title":"Conductive supramolecular acrylate hydrogels enabled by quaternized chitosan ionic crosslinking for high-fidelity 3D printing","authors":"Lukas A. Bauman, Boxin Zhao","doi":"10.1016/j.carpta.2025.100702","DOIUrl":"10.1016/j.carpta.2025.100702","url":null,"abstract":"<div><div>While 3D printing has enabled the fabrication of hydrogels with complex structures, high fidelity techniques (vat polymerization) that enable precisely engineered design of hydrogels require stiff structures to withstand the forces of printing. This is a pressing research gap in hydrogel vat-polymerization 3D printing. To address this limitation, a novel ionic crosslinker consisting of quaternized chitosan complexed with 3-sulfopropyl acrylate was used to form supramolecular 2-hydroxyethyl acrylate organogel precursors. The Cyrene organogel enhanced mechanical properties enabling the printing of high-fidelity structures; the final compliant hydrogels were then obtained through solvent exchange with water. This yielded high-fidelity 3D-printed conductive supramolecular hydrogels with tensile properties of 288±29 kPa at 516±37 % elongation and compressive properties of 572±34 kPa at 65±4 % strain with uniform swelling (320–350 %). Nuclear magnetic resonance and conductivity measurements confirmed SPA-rich blocks within the hydrogel network and the solvent-dependent copolymer structure. Furthermore, through varying the anionic acrylate concentration, ultimate strain between 222 % and 516 % was achieved at a constant elastic modulus. Additionally, electrical properties were tunable with conductivity reaching 156 mS/m at 7 MH in ultrapure water. This work advances applications of quaternized chitosan as an ionic crosslinker in printable conductive hydrogels, opening new applications in medical and technological fields.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100702"},"PeriodicalIF":6.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable multi-purpose bacterial cellulose composite for food packaging via facile successive infiltration

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-04 DOI: 10.1016/j.carpta.2025.100703

I-Tseng Liu, Cheng-Ying Li, Ying-Chih Liao

Petroleum-based plastic packaging materials have been widely used, causing severe environmental impacts. To address this challenge, there is an urgent need to develop biodegradable materials that offer strong mechanical and excellent barrier properties while ensuring food safety. In this study, sustainable, high-performance film derived from bacterial cellulose (BC) through a straightforward and eco-friendly water-based successive infiltration process is developed. To enhance the transparency and haze of BC specimens for better visual detection, waterborne polyurethane (WPU) is infiltrated for refractive index compensation and serves as an adhesive. Subsequently, the mechanical strength and water resistance of the BC/WPU films are improved by incorporating a seaweed extract, sodium alginate (SA), and metal ion chelation (Ca²⁺, Al³⁺, and Zr⁴⁺). The resulting BC/WPU/[email protected] film exhibited low hygroscopicity (+53.5 % after 190 h of immersion), high transparency (90.01 %) and excellent haze (10.91 %), exceptional tensile strength (82.8 MPa) and modulus (6.96 GPa), low gas permeability (OP = 0.0137 mL-mm/m²-day-atm and WVP = 8.75 g-mm/m²-day-atm), high biodegradability (85.23 % weight loss in 49 days), high flexibility, formability, and great heat sealability. These outstanding features make the BC/WPU/[email protected] film exceptionally well-suited for advanced and versatile packaging applications. Several examples were also demonstrated to show its exceptional potential for packaging applications.

{"title":"Sustainable multi-purpose bacterial cellulose composite for food packaging via facile successive infiltration","authors":"I-Tseng Liu, Cheng-Ying Li, Ying-Chih Liao","doi":"10.1016/j.carpta.2025.100703","DOIUrl":"10.1016/j.carpta.2025.100703","url":null,"abstract":"<div><div>Petroleum-based plastic packaging materials have been widely used, causing severe environmental impacts. To address this challenge, there is an urgent need to develop biodegradable materials that offer strong mechanical and excellent barrier properties while ensuring food safety. In this study, sustainable, high-performance film derived from bacterial cellulose (BC) through a straightforward and eco-friendly water-based successive infiltration process is developed. To enhance the transparency and haze of BC specimens for better visual detection, waterborne polyurethane (WPU) is infiltrated for refractive index compensation and serves as an adhesive. Subsequently, the mechanical strength and water resistance of the BC/WPU films are improved by incorporating a seaweed extract, sodium alginate (SA), and metal ion chelation (Ca<sup>2+</sup>, Al<sup>3+</sup>, and Zr<sup>4+</sup>). The resulting BC/WPU/[email protected] film exhibited low hygroscopicity (+53.5 % after 190 h of immersion), high transparency (90.01 %) and excellent haze (10.91 %), exceptional tensile strength (82.8 MPa) and modulus (6.96 GPa), low gas permeability (OP = 0.0137 mL-mm/m²-day-atm and WVP = 8.75 g-mm/m²-day-atm), high biodegradability (85.23 % weight loss in 49 days), high flexibility, formability, and great heat sealability. These outstanding features make the BC/WPU/[email protected] film exceptionally well-suited for advanced and versatile packaging applications. Several examples were also demonstrated to show its exceptional potential for packaging applications.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100703"},"PeriodicalIF":6.2,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-03 DOI: 10.1016/j.carpta.2025.100695

Asghar Narmani , Saeid Ganji , Maryam Amirishoar , Fatemeh Hajikarimi , Aynaz Mazandarani , Mahya Karimi , Ali Mohammadinejad , Azin Azadpour , Roghayyeh Jahedi , Elham Assadpour , Seid Mahdi Jafari

Breast cancer has a high rate of incidence and is one of the leading causes of death worldwide. Therefore, a breakthrough aptamer (Apt)- and folic acid (FA)-targeted chitosan (CS)-poly lactic-co-glycolic acid (PLGA) nanocarrier was developed for co-delivery of sorafenib (So) and quercetin (Qu) to MCF-7 and MDA-MB-231 breast cancer cells. Nanometric size (50 to 110 nm), semi-spherical and spherical shape, rough surface, and near-to-neutral surface charge (3.8 mV) were measured for Apt-PLGA-So-Qu-CS-FA nanocarriers. The So and Qu drugs content was obtained at about 2.85% and 11.63%, respectively. Controlled release (6.3% (So) and 7.2% (Qu) within 2 h) and pH-sensitive drug release was observed for this nanocarrier. MTT assay indicated lower cell viability for MCF-7 cells after treatment with Apt-PLGA-So-Qu-CS-FA nanocarriers (10% cell viability after 24 h treatment with 250 nm; IC₅₀ = 100 nm). Caspase9 and P53 genes expression was increased by 11.8 and 12.8 folds while > 5 folds reduction was observed for Bcl2 expression after treatment with Apt-PLGA-So-Qu-CS-FA. Also, this nanocarrier led to > 90% proptosis and > 10- and 11.5-fold enhancement in SOD and catalase values in MCF-7 cells. Cellular uptake was about 100%, 77%, and 0.5% for MCF-7 cells treated with Apt-PLGA-CS-FA, PLGA-CS-FA, and CS nanocarriers, respectively which shows the impact of dual-targeting. The fabricated dual targeted nanodelivery system would be a potential device against breast cancer.

{"title":"Dual targeting of breast cancer by chitosan/poly lactic-co-glycolic acid nanodelivery systems: Surface activation with folic acid/aptamers, and co-encapsulated with Sorafenib and quercetin","authors":"Asghar Narmani , Saeid Ganji , Maryam Amirishoar , Fatemeh Hajikarimi , Aynaz Mazandarani , Mahya Karimi , Ali Mohammadinejad , Azin Azadpour , Roghayyeh Jahedi , Elham Assadpour , Seid Mahdi Jafari","doi":"10.1016/j.carpta.2025.100695","DOIUrl":"10.1016/j.carpta.2025.100695","url":null,"abstract":"<div><div>Breast cancer has a high rate of incidence and is one of the leading causes of death worldwide. Therefore, a breakthrough aptamer (Apt)- and folic acid (FA)-targeted chitosan (CS)-poly lactic-co-glycolic acid (PLGA) nanocarrier was developed for co-delivery of sorafenib (So) and quercetin (Qu) to MCF-7 and MDA-MB-231 breast cancer cells. Nanometric size (50 to 110 nm), semi-spherical and spherical shape, rough surface, and near-to-neutral surface charge (3.8 mV) were measured for Apt-PLGA-So-Qu-CS-FA nanocarriers. The So and Qu drugs content was obtained at about 2.85% and 11.63%, respectively. Controlled release (6.3% (So) and 7.2% (Qu) within 2 h) and pH-sensitive drug release was observed for this nanocarrier. MTT assay indicated lower cell viability for MCF-7 cells after treatment with Apt-PLGA-So-Qu-CS-FA nanocarriers (10% cell viability after 24 h treatment with 250 nm; IC<sub>50</sub> = 100 nm). Caspase9 and P53 genes expression was increased by 11.8 and 12.8 folds while > 5 folds reduction was observed for Bcl2 expression after treatment with Apt-PLGA-So-Qu-CS-FA. Also, this nanocarrier led to > 90% proptosis and > 10- and 11.5-fold enhancement in SOD and catalase values in MCF-7 cells. Cellular uptake was about 100%, 77%, and 0.5% for MCF-7 cells treated with Apt-PLGA-CS-FA, PLGA-CS-FA, and CS nanocarriers, respectively which shows the impact of dual-targeting. The fabricated dual targeted nanodelivery system would be a potential device against breast cancer.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100695"},"PeriodicalIF":6.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incorporating insulin into alginate-chitosan 3D-printed scaffolds: A comprehensive study on structure, mechanics, and biocompatibility for cartilage tissue engineering

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-03 DOI: 10.1016/j.carpta.2025.100696

Afsaneh Jahani , Mohammad Sadegh Nourbakhsh , Ali Moradi , Marzieh Mohammadi , Lobat Tayebi

Osteoarthritis is a leading cause of disability worldwide, challenging current treatments to limited cartilage self-healing capacity. Cartilage tissue engineering (CTE) integrates cells, scaffolds, and signaling molecules, with Insulin being utilized as a differentiation biomolecule due to cost-effectiveness, dose-dependent influence on chondrogenesis, suitable biological activity, and ability to activate relevant receptors. Yet, administering differentiation biomolecules through conventional scaffolds poses a persistent challenge. Alginate (Alg) is commonly employed in CTE for its biocompatibility, though it lacks sufficient mechanical properties. Chitosan (Cs), while enhancing scaffold mechanical properties, but does not independently provide optimal support for chondrogenesis. While Alg-Cs scaffolds have garnered attention, challenges persist in achieving sustained differentiation biomolecules delivery and attaining suboptimal structural and biological properties for cartilage regeneration. This study utilizes advanced scaffolds by employing three dimensional (3D) printing technique to create Insulin-loaded Alg-Cs scaffolds, examining their structural, mechanical, and release properties, and assessing cell viability and chondrogenic differentiation through markers like COL1A1, COL2A1, SOX9 and ACAN. Following a 30-day implantation period, we also evaluate histological parameters. The findings revealed that the incorporation of a 20 (μg/ml) dose of Insulin into Alg-Cs 3D-printed scaffolds significantly enhanced the expression of these markers, indicating improved chondrogenic potential for cartilage regeneration. Histological analysis confirmed favorable biocompatibility and structural integrity of Insulin-loaded pure Alg and Alg-Cs scaffolds at drug loading levels of up to 20 and 10 μg, respectively. The hypothesis suggests that these advanced scaffolds can achieve controlled Insulin release, enhancing cartilage regeneration. This research aims to develop to yield mechanically optimized, bioactive 3D-printed scaffolds for regulated delivery of Insulin to promote cartilage regeneration.

{"title":"Incorporating insulin into alginate-chitosan 3D-printed scaffolds: A comprehensive study on structure, mechanics, and biocompatibility for cartilage tissue engineering","authors":"Afsaneh Jahani , Mohammad Sadegh Nourbakhsh , Ali Moradi , Marzieh Mohammadi , Lobat Tayebi","doi":"10.1016/j.carpta.2025.100696","DOIUrl":"10.1016/j.carpta.2025.100696","url":null,"abstract":"<div><div>Osteoarthritis is a leading cause of disability worldwide, challenging current treatments to limited cartilage self-healing capacity. Cartilage tissue engineering (CTE) integrates cells, scaffolds, and signaling molecules, with Insulin being utilized as a differentiation biomolecule due to cost-effectiveness, dose-dependent influence on chondrogenesis, suitable biological activity, and ability to activate relevant receptors. Yet, administering differentiation biomolecules through conventional scaffolds poses a persistent challenge. Alginate (Alg) is commonly employed in CTE for its biocompatibility, though it lacks sufficient mechanical properties. Chitosan (Cs), while enhancing scaffold mechanical properties, but does not independently provide optimal support for chondrogenesis. While Alg-Cs scaffolds have garnered attention, challenges persist in achieving sustained differentiation biomolecules delivery and attaining suboptimal structural and biological properties for cartilage regeneration. This study utilizes advanced scaffolds by employing three dimensional (3D) printing technique to create Insulin-loaded Alg-Cs scaffolds, examining their structural, mechanical, and release properties, and assessing cell viability and chondrogenic differentiation through markers like COL1A1, COL2A1, SOX9 and ACAN. Following a 30-day implantation period, we also evaluate histological parameters. The findings revealed that the incorporation of a 20 (μg/ml) dose of Insulin into Alg-Cs 3D-printed scaffolds significantly enhanced the expression of these markers, indicating improved chondrogenic potential for cartilage regeneration. Histological analysis confirmed favorable biocompatibility and structural integrity of Insulin-loaded pure Alg and Alg-Cs scaffolds at drug loading levels of up to 20 and 10 μg, respectively. The hypothesis suggests that these advanced scaffolds can achieve controlled Insulin release, enhancing cartilage regeneration. This research aims to develop to yield mechanically optimized, bioactive 3D-printed scaffolds for regulated delivery of Insulin to promote cartilage regeneration.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100696"},"PeriodicalIF":6.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorptive removal of toxic heavy metal and dyes from wastewater by rice husk (lignocellulosic biomass) derived activated biochar: A fixed-bed column adsorption study

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-03 DOI: 10.1016/j.carpta.2025.100698

Md. Mahmudur Rahman , Mohd. Maniruzzaman , Nor ealam , Parvej Mahmud , Sumiya Khatun , Md. Kaowsar Hossain , Md. Ismail Hossain , Md. Hasanuzzaman , Md. Ashraful Alam , Md. Al-amin , Bijoy Chandra Ghos

Rapid industrialization/urbanization has been marked as a responsible factor for the creation of bulky effluent which is loaded with several harmful toxicants resulting in a hursh damage to the ecosystem and public safety/secuerity. To mitigate this crucial issue, here a fixed-bed column adsorption technology were chosen due to its simplecity, cost-effectivity, reuseablity. Meanwhile, multifunctional biosorbent i.e functionallized rice husk biochar (FRHBC) was produced by EIPS technique from a beneficial source namely rice(Oriza sativa) waste shell. The experiments were conducted to optimize the effect of influential factors like concentration, flowrates, and bed height. To observe the bulk properties the samples were characterized by FESEM,EDX,BET, FTIR-ATR,XRD, and TGA analysis. The effluents were investigated by AAS and UV–vis-NIR techniques. As per the findings it has been stated that the synthesized FRHBC adsorbents have possessed a promizing 2D honeycomb-like well-oriented mesoporous surface microstructure with a larger surface area around 36.84 m²/g. Additionally holds substantial active binding sites, lower crystallinity nearly 23±0.03%, and higher thermal stability upto 700 °C. Noteworthy that the maximum adsorption performence was found 179.77 and 173.01 mg/g for Pb²⁺ and MB dye. Hence, the newly produced multifunctional FRHBC bioadsorbents would be benificially used to purify real-time industrial wastewater for sustainable environmental protection.

{"title":"Adsorptive removal of toxic heavy metal and dyes from wastewater by rice husk (lignocellulosic biomass) derived activated biochar: A fixed-bed column adsorption study","authors":"Md. Mahmudur Rahman , Mohd. Maniruzzaman , Nor ealam , Parvej Mahmud , Sumiya Khatun , Md. Kaowsar Hossain , Md. Ismail Hossain , Md. Hasanuzzaman , Md. Ashraful Alam , Md. Al-amin , Bijoy Chandra Ghos","doi":"10.1016/j.carpta.2025.100698","DOIUrl":"10.1016/j.carpta.2025.100698","url":null,"abstract":"<div><div>Rapid industrialization/urbanization has been marked as a responsible factor for the creation of bulky effluent which is loaded with several harmful toxicants resulting in a hursh damage to the ecosystem and public safety/secuerity. To mitigate this crucial issue, here a fixed-bed column adsorption technology were chosen due to its simplecity, cost-effectivity, reuseablity. Meanwhile, multifunctional biosorbent <em>i.e</em> functionallized rice husk biochar (FRHBC) was produced by EIPS technique from a beneficial source namely rice(<em>Oriza sativa</em>) waste shell. The experiments were conducted to optimize the effect of influential factors like concentration, flowrates, and bed height. To observe the bulk properties the samples were characterized by FESEM,EDX,BET, FTIR-ATR,XRD, and TGA analysis. The effluents were investigated by AAS and UV–vis-NIR techniques. As per the findings it has been stated that the synthesized FRHBC adsorbents have possessed a promizing 2D honeycomb-like well-oriented mesoporous surface microstructure with a larger surface area around 36.84 m<sup>2</sup>/g. Additionally holds substantial active binding sites, lower crystallinity nearly 23±0.03%, and higher thermal stability upto 700 °C. Noteworthy that the maximum adsorption performence was found 179.77 and 173.01 mg/g for Pb<sup>2+</sup> and MB dye. Hence, the newly produced multifunctional FRHBC bioadsorbents would be benificially used to purify real-time industrial wastewater for sustainable environmental protection.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100698"},"PeriodicalIF":6.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into selection and application of carbohydrate-based carriers for microencapsulation: Stability and functional properties of maltodextrin, gum Arabic, and β-cyclodextrin in encapsulating tea flower pollen peptides

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-03 DOI: 10.1016/j.carpta.2025.100700

Pegah Mehrali , Seyed Hadi Peighambardoust , Amir Akbarmehr , Khashayar Sarabandi

Protein from tea flower pollen (TFP) was extracted and hydrolyzed to achieve polypeptides. The produced peptides were rich in hydrophobic (∼44 %), antioxidants (∼19 %), and essential (∼49 %) amino acids. Various carriers, including maltodextrin (MD), gum Arabic (GA), and β-cyclodextrin (βCD), along with their combinations, were utilized for the encapsulation purpose. The highest spray-drying yield (∼59 %) was achieved when a combination of MD-βCD was used as carrier. The physical characteristics of spray-dried (SD) powders including bulk and tapped densities (packing ability), Hassner ratio, angle of repose and Carr index (powder flowability) were influenced by the type of carriers used. The maximum solubility (88 %) and wettability (333 s) values were obtained for SD-powders produced by MD-GA as carrier. The encapsulation of peptides using MD-βCD caused a significant decrease in the hygroscopicity (from ∼85 to 39 %), indicating their higher physical stability compared to other carriers. The highest antioxidant activity in inhibiting DPPH⁻ (∼52.1 %) and ABTS⁺ (∼62.5 %) radicals was obtained in peptides stabilized with MD-βCD. Morphological features of SD-powders were affected by the type of carriers. SEM showed that utilizing MD-βCD as carrier leads to formation of spherical particles with smooth surface with less pore formation. FT-IR showed the coverage of amide regions and functional groups of peptides and indicated their placement and distribution within the carrier matrix. This study highlights the potential of using microencapsulated tea flower pollen peptides as a functional ingredient for food and nutraceutical applications.

{"title":"Insights into selection and application of carbohydrate-based carriers for microencapsulation: Stability and functional properties of maltodextrin, gum Arabic, and β-cyclodextrin in encapsulating tea flower pollen peptides","authors":"Pegah Mehrali , Seyed Hadi Peighambardoust , Amir Akbarmehr , Khashayar Sarabandi","doi":"10.1016/j.carpta.2025.100700","DOIUrl":"10.1016/j.carpta.2025.100700","url":null,"abstract":"<div><div>Protein from tea flower pollen (TFP) was extracted and hydrolyzed to achieve polypeptides. The produced peptides were rich in hydrophobic (∼44 %), antioxidants (∼19 %), and essential (∼49 %) amino acids. Various carriers, including maltodextrin (MD), gum Arabic (GA), and β-cyclodextrin (βCD), along with their combinations, were utilized for the encapsulation purpose. The highest spray-drying yield (∼59 %) was achieved when a combination of MD-βCD was used as carrier. The physical characteristics of spray-dried (SD) powders including bulk and tapped densities (packing ability), Hassner ratio, angle of repose and Carr index (powder flowability) were influenced by the type of carriers used. The maximum solubility (88 %) and wettability (333 s) values were obtained for SD-powders produced by MD-GA as carrier. The encapsulation of peptides using MD-βCD caused a significant decrease in the hygroscopicity (from ∼85 to 39 %), indicating their higher physical stability compared to other carriers. The highest antioxidant activity in inhibiting DPPH<sup>−</sup> (∼52.1 %) and ABTS<sup>+</sup> (∼62.5 %) radicals was obtained in peptides stabilized with MD-βCD. Morphological features of SD-powders were affected by the type of carriers. SEM showed that utilizing MD-βCD as carrier leads to formation of spherical particles with smooth surface with less pore formation. FT-IR showed the coverage of amide regions and functional groups of peptides and indicated their placement and distribution within the carrier matrix. This study highlights the potential of using microencapsulated tea flower pollen peptides as a functional ingredient for food and nutraceutical applications.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100700"},"PeriodicalIF":6.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143360556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0