Carbohydrate Polymer Technologies and Applications最新文献_第3页

Cellulose nanocrystal (CNC) from okra plant (Abelmoschus esculentus L.) stalks as a reinforcement in bionanocomposite fabrication: Extraction, processing, and characterization study 将秋葵（Abelmoschus esculentus L.）茎秆中的纤维素纳米晶（CNC）作为仿生复合材料制造中的增强材料：提取、加工和表征研究

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-10-05 DOI: 10.1016/j.carpta.2024.100581

Md.Mahmudur Rahman , Md.Ismail Hossain , Md.Mahafujul Hassan , Bijoy Chandra Ghos , Md.Saifur Rahman , Md.Abdul Gafur , Md.Ashraful Alam , Md.Khalid Al Zuhanee

Currently, CNC is attractive to the researchers to fabricate multifunctional bionanocomposite because of their outstanding physicochemical, thermomechanical, morphological properties, and eco-friendly nature. Whereas in most cases CNC is extracted from the bast/bark of primary plants, which have other beneficial applications in several sectors. Hence, it is crucial to find out alternative sources of CNCs to diminish the extra pressure on the primary plants. While the useless agrowaste biomass of okra stalks would be a new and beneficial alternative to produce CNCs as a reinforcement. Here, a series of chemical reactions were directed to produce CNCs. The samples were characterized by FTIR-ATR,TGA/DTA,FESEM,EDX,XRD,UV–vis-NIR, and DLS analysis. The obtained results suggested that the newly produced CNCs possessed substantial active functional groups(–OH,CO-C,–NH), high thermal stability, greater crystallinity(86.09±0.001 %), and notable microstructure indicating a well-organized porous surface with encouraging spherical shapes. The CNCs are free from impurities and coloring materials; additionally, they exhibit a highly negative surface charge and smaller particle sizes. It can be stated that the produced CNCs should have a good agreement with the sustainable environment and be beneficially applied as a reinforcing agent to fabricate bionanocomposites. Also acts as a sustainable alternative to the fossil-based hazardous ones for various uses.

目前，氯化萘因其出色的物理化学、热力学、形态学特性和生态友好性质，吸引着研究人员制造多功能仿生复合材料。而在大多数情况下，氯化萘是从初级植物的韧皮部/树皮中提取的，而初级植物的韧皮部/树皮在多个领域都有其他有益的应用。因此，找到氯化萘的替代来源以减少对原生植物的额外压力至关重要。而秋葵茎秆这种无用的农业废弃物生物质将成为生产氯化萘的一种新的有益替代品。在此，我们通过一系列化学反应生产出氯化萘。样品通过傅立叶变换红外-原子吸收光谱（FTIR-ATR）、热重分析（TGA）/热重分析（DTA）、外延电子显微镜（FESEM）、电离辐射分析（EDX）、X射线衍射（XRD）、紫外-可见-近红外（UV-vis-NIR）和光致发光（DLS）分析进行表征。结果表明，新制备的 CNC 具有大量的活性官能团（-OH、CO-C、-NH）、高热稳定性、较高的结晶度（86.09±0.001 %）以及显著的微观结构，表明其表面具有组织良好的多孔性和令人鼓舞的球形。这些 CNC 不含杂质和着色材料，而且表面电荷为负，粒径较小。可以说，生产出的 CNC 与可持续发展的环境十分吻合，可用作制造仿生复合材料的增强剂。此外，它还是化石基有害物质的可持续替代品，可用于多种用途。

{"title":"Cellulose nanocrystal (CNC) from okra plant (Abelmoschus esculentus L.) stalks as a reinforcement in bionanocomposite fabrication: Extraction, processing, and characterization study","authors":"Md.Mahmudur Rahman , Md.Ismail Hossain , Md.Mahafujul Hassan , Bijoy Chandra Ghos , Md.Saifur Rahman , Md.Abdul Gafur , Md.Ashraful Alam , Md.Khalid Al Zuhanee","doi":"10.1016/j.carpta.2024.100581","DOIUrl":"10.1016/j.carpta.2024.100581","url":null,"abstract":"<div><div>Currently, CNC is attractive to the researchers to fabricate multifunctional bionanocomposite because of their outstanding physicochemical, thermomechanical, morphological properties, and eco-friendly nature. Whereas in most cases CNC is extracted from the bast/bark of primary plants, which have other beneficial applications in several sectors. Hence, it is crucial to find out alternative sources of CNCs to diminish the extra pressure on the primary plants. While the useless agrowaste biomass of okra stalks would be a new and beneficial alternative to produce CNCs as a reinforcement. Here, a series of chemical reactions were directed to produce CNCs. The samples were characterized by FTIR-ATR,TGA/DTA,FESEM,EDX,XRD,UV–vis-NIR, and DLS analysis. The obtained results suggested that the newly produced CNCs possessed substantial active functional groups(–OH,C<img>O-C,–NH), high thermal stability, greater crystallinity(86.09±0.001 %), and notable microstructure indicating a well-organized porous surface with encouraging spherical shapes. The CNCs are free from impurities and coloring materials; additionally, they exhibit a highly negative surface charge and smaller particle sizes. It can be stated that the produced CNCs should have a good agreement with the sustainable environment and be beneficially applied as a reinforcing agent to fabricate bionanocomposites. Also acts as a sustainable alternative to the fossil-based hazardous ones for various uses.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100581"},"PeriodicalIF":6.2,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of eco-friendly cathode materials from cellulose skeleton induced manganese dioxide to produce zinc ion battery: Physicochemical, morphological, and electrochemical study 利用纤维素骨架诱导二氧化锰生产锌离子电池的环保型正极材料：物理化学、形态学和电化学研究

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-10-05 DOI: 10.1016/j.carpta.2024.100583

Md.Ismail Hossain, Md.Mahmudur Rahman

Energy security is a universal issue since modern civilization cannot be imagined for a single moment without energy. Though lean fossil fuels are fighting against the current energy demand principally, these are linked to adverse environmental effects. Various energy storage technologies can resolve this issue in a sustainable eco-friendly manner. Rechargeable Zn-ion batteries (ZIBs) are emerging as potential candidates to meet the growing commercial demand with several advantages. As a part of this, the cellulose skeleton-induced manganese dioxide-based electroactive cathode materials have been prepared by an innovative route and characterized using UV–vis-NIR, FTIR, FESEM, EDS, and XRD analysis. Results suggested that the newly produced eco-friendly cathode materials have possessed outstanding physicochemical, morphological, and structural properties. While the finished product namely the coin cell of ZIBs has shown a good CV profile along with minimal charge transfer resistance in PEIS. Noteworthy that the newly prepared cathode materials have also been shown a significant capacity of 29 mAhg^-1 at a 5 mA constant current charge-discharge rate. Thus, the method could be beneficially used in energy storage devices for its eco-friendly nature though some drawbacks have been noticed. The cyclic stabilities were somewhat low which can be increased in further studies.

能源安全是一个普遍问题，因为现代文明一刻也离不开能源。尽管化石燃料主要用于满足当前的能源需求，但这些燃料会对环境造成不利影响。各种储能技术可以以可持续的生态友好方式解决这一问题。可充电锌离子电池（ZIBs）具有多种优势，正在成为满足日益增长的商业需求的潜在候选产品。作为其中的一部分，我们采用创新路线制备了纤维素骨架诱导的二氧化锰基电活性阴极材料，并使用紫外可见-近红外光谱、傅立叶变换红外光谱、外聚焦电子显微镜、电致发光分析和 X 射线衍射分析对其进行了表征。结果表明，新制备的环保型阴极材料具有出色的物理化学、形态和结构特性。成品（即 ZIBs 的纽扣电池）在 PEIS 中显示出良好的 CV 曲线和最小的电荷转移电阻。值得注意的是，新制备的阴极材料在 5 mA 恒定电流充放电速率下也显示出 29 mAhg-1 的显著容量。因此，虽然这种方法还存在一些缺点，但由于其环保性，可用于储能设备中。循环稳定性略低，有待进一步研究提高。

{"title":"Production of eco-friendly cathode materials from cellulose skeleton induced manganese dioxide to produce zinc ion battery: Physicochemical, morphological, and electrochemical study","authors":"Md.Ismail Hossain, Md.Mahmudur Rahman","doi":"10.1016/j.carpta.2024.100583","DOIUrl":"10.1016/j.carpta.2024.100583","url":null,"abstract":"<div><div>Energy security is a universal issue since modern civilization cannot be imagined for a single moment without energy. Though lean fossil fuels are fighting against the current energy demand principally, these are linked to adverse environmental effects. Various energy storage technologies can resolve this issue in a sustainable eco-friendly manner. Rechargeable Zn-ion batteries (ZIBs) are emerging as potential candidates to meet the growing commercial demand with several advantages. As a part of this, the cellulose skeleton-induced manganese dioxide-based electroactive cathode materials have been prepared by an innovative route and characterized using UV–vis-NIR, FTIR, FESEM, EDS, and XRD analysis. Results suggested that the newly produced eco-friendly cathode materials have possessed outstanding physicochemical, morphological, and structural properties. While the finished product namely the coin cell of ZIBs has shown a good CV profile along with minimal charge transfer resistance in PEIS. Noteworthy that the newly prepared cathode materials have also been shown a significant capacity of 29 mAhg<sup>-1</sup> at a 5 mA constant current charge-discharge rate. Thus, the method could be beneficially used in energy storage devices for its eco-friendly nature though some drawbacks have been noticed. The cyclic stabilities were somewhat low which can be increased in further studies.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100583"},"PeriodicalIF":6.2,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of collagen-sodium alginate based antibacterial and edible packaging material: Performance evaluation using Entropy-Combined Compromise Solution (CoCoSo) 制作基于胶原蛋白-海藻酸钠的抗菌可食用包装材料：使用熵-组合折衷方案（CoCoSo）进行性能评估

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-10-04 DOI: 10.1016/j.carpta.2024.100582

Mysha Maliha , Taslim Ur Rashid , Mohammed Mizanur Rahman

In this research, we developed two alternative packaging materials: collagen-sodium alginate-pomegranate peel powder (CSP) and collagen-sodium alginate-chitosan (CSC), leveraging solid waste from both the leather and food industries. A comparative analysis was done between CSP and CSC film to evaluate their efficacy and potentiality as sustainable packaging material. CSC film showed reduced water solubility (39.4 %), water vapor transmission rate (27.3 gh^-1m^-2), and opacity (0.6 A₆₀₀/mm) along with improved contact angle (69.7°) compared to CSP film. However, CSP film exhibited improved mechanical strength (10.9 MPa) and biodegradability (72.1 %) along with reduced oxygen permeability (0.6 meq/kg) compared to CSC film. Furthermore, CSP and CSC films showed significant inhibitory effect against two commonly available pathogen strains. A hybrid Entropy-Combined Compromise Solution (CoCoSo) analysis results demonstrated the superior performance of CSC films (γi=3.049) as packaging material. The edibility of the film was confirmed through pathogen test, in vitro cytotoxicity and stimulated digestibility assays. Additionally, from the thermal analysis it is apparent that the optimum degradation temperature for CSC film was 584 °C which is higher than any other green packaging material reported in the literature. Therefore, this study can pave the way for the fabrication of thermally stable edible packaging material from waste sources.

在这项研究中，我们开发了两种替代包装材料：胶原蛋白-海藻酸钠-石榴皮粉（CSP）和胶原蛋白-海藻酸钠-壳聚糖（CSC），利用的是皮革业和食品业的固体废弃物。我们对 CSP 和 CSC 薄膜进行了比较分析，以评估它们作为可持续包装材料的功效和潜力。与 CSP 薄膜相比，CSC 薄膜的水溶性（39.4%）、水蒸气透过率（27.3 gh-1m-2）和不透明度（0.6 A600/mm）均有所降低，接触角（69.7°）也有所改善。不过，与 CSC 薄膜相比，CSP 薄膜的机械强度（10.9 兆帕）和生物降解性（72.1%）都有所提高，氧气渗透率（0.6 meq/kg）也有所降低。此外，CSP 和 CSC 薄膜对两种常见的病原体菌株有显著的抑制作用。熵-综合折衷方案（CoCoSo）混合分析结果表明，CSC 薄膜（γi=3.049）作为包装材料具有更优越的性能。通过病原体试验、体外细胞毒性和刺激消化试验，证实了薄膜的可食性。此外，热分析表明，CSC 薄膜的最佳降解温度为 584 ℃，高于文献中报道的任何其他绿色包装材料。因此，这项研究可以为利用废物制造热稳定性可食用包装材料铺平道路。

{"title":"Fabrication of collagen-sodium alginate based antibacterial and edible packaging material: Performance evaluation using Entropy-Combined Compromise Solution (CoCoSo)","authors":"Mysha Maliha , Taslim Ur Rashid , Mohammed Mizanur Rahman","doi":"10.1016/j.carpta.2024.100582","DOIUrl":"10.1016/j.carpta.2024.100582","url":null,"abstract":"<div><div>In this research, we developed two alternative packaging materials: collagen-sodium alginate-pomegranate peel powder (CSP) and collagen-sodium alginate-chitosan (CSC), leveraging solid waste from both the leather and food industries. A comparative analysis was done between CSP and CSC film to evaluate their efficacy and potentiality as sustainable packaging material. CSC film showed reduced water solubility (39.4 %), water vapor transmission rate (27.3 gh<sup>-1</sup>m<sup>-2</sup>), and opacity (0.6 A<sub>600</sub>/mm) along with improved contact angle (69.7°) compared to CSP film. However, CSP film exhibited improved mechanical strength (10.9 MPa) and biodegradability (72.1 %) along with reduced oxygen permeability (0.6 meq/kg) compared to CSC film. Furthermore, CSP and CSC films showed significant inhibitory effect against two commonly available pathogen strains. A hybrid Entropy-Combined Compromise Solution (CoCoSo) analysis results demonstrated the superior performance of CSC films (γi=3.049) as packaging material. The edibility of the film was confirmed through pathogen test, <em>in vitro</em> cytotoxicity and stimulated digestibility assays. Additionally, from the thermal analysis it is apparent that the optimum degradation temperature for CSC film was 584 °C which is higher than any other green packaging material reported in the literature. Therefore, this study can pave the way for the fabrication of thermally stable edible packaging material from waste sources.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100582"},"PeriodicalIF":6.2,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Chitosan-Maize Starch based Co-processed excipient: As release retardant material 开发壳聚糖-玉米淀粉共处理辅料：作为缓释材料

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-10-02 DOI: 10.1016/j.carpta.2024.100580

Smit Patel, Dinal Patel, Himani Patel, Dr. Mansi Dholakia, Dr. Tejal Soni

In the present study, co-processed excipients from chitosan, and maize starch were prepared by co-dispersion, and co-granulation methods in 1:1 ratio. The resultant products were then characterized physically by their micrometric properties and hygroscopic characters. The co-processed excipient prepared by co-granulation was chosen and was added to a directly compressible oral sustained-release tablet (Batch F1-F4) containing ibuprofen as a model drug. A well-known marketed ibuprofen tablet (Motrin) was taken for comparison purposes of the dissolution characteristics. Pre-compression parameters, as well as post-compression parameters of all the batches, were performed. The pre-compression parameters, and post-compression parameters of F3, containing chitosan to maize in the ratio of 2:1, were found to be comparable with the marketed product. F3 batch showed 74.7 % of drug release up to 8 h, which reflect the release retardant properties of co-processed excipient. Collectively, co-processed excipients of chitosan, and maize starch improve the physical properties of individual materials, and can be used as a directly compressible excipient as a release retardant material for oral sustained-release tablets.

本研究采用共分散和共制粒方法，以 1:1 的比例制备了壳聚糖和玉米淀粉共处理辅料。然后通过微量特性和吸湿特性对所得产品进行物理表征。选择共制粒法制备的共加工辅料，并将其添加到含有布洛芬作为模型药物的直接可压缩口服缓释片（批次 F1-F4）中。为了比较溶出特性，采用了一种知名的市场上销售的布洛芬片剂（Motrin）。对所有批次的压缩前参数和压缩后参数进行了测定。结果发现，含有壳聚糖和玉米（比例为 2:1）的 F3 批次的压缩前参数和压缩后参数与市售产品相当。F3 批次在 8 小时内的药物释放率为 74.7%，这反映了共处理辅料的缓释特性。总之，壳聚糖和玉米淀粉共加工辅料改善了单个材料的物理性质，可作为直接可压缩辅料作为缓释材料用于口服缓释片。

{"title":"Development of Chitosan-Maize Starch based Co-processed excipient: As release retardant material","authors":"Smit Patel, Dinal Patel, Himani Patel, Dr. Mansi Dholakia, Dr. Tejal Soni","doi":"10.1016/j.carpta.2024.100580","DOIUrl":"10.1016/j.carpta.2024.100580","url":null,"abstract":"<div><div>In the present study, co-processed excipients from chitosan, and maize starch were prepared by co-dispersion, and co-granulation methods in 1:1 ratio. The resultant products were then characterized physically by their micrometric properties and hygroscopic characters. The co-processed excipient prepared by co-granulation was chosen and was added to a directly compressible oral sustained-release tablet (Batch F1-F4) containing ibuprofen as a model drug. A well-known marketed ibuprofen tablet (Motrin) was taken for comparison purposes of the dissolution characteristics. Pre-compression parameters, as well as post-compression parameters of all the batches, were performed. The pre-compression parameters, and post-compression parameters of F3, containing chitosan to maize in the ratio of 2:1, were found to be comparable with the marketed product. F3 batch showed 74.7 % of drug release up to 8 h, which reflect the release retardant properties of co-processed excipient. Collectively, co-processed excipients of chitosan, and maize starch improve the physical properties of individual materials, and can be used as a directly compressible excipient as a release retardant material for oral sustained-release tablets.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100580"},"PeriodicalIF":6.2,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive matrix scaffold of oxidized sacchachitin via green catalyst of siliacat tempo for enhancing bone regeneration 通过绿色催化剂 Siliacat tempo 氧化囊壳素的生物活性基质支架用于促进骨再生

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-09-30 DOI: 10.1016/j.carpta.2024.100576

Ming-Thau Sheu , Shyr-Yi Lin , Wei-Jie Cheng , Hong-Liang Lin , Chien-Ming Hsieh , Hsiu-O Ho , Ling-Chun Chen , Meng-Huang Wu

Bone regeneration remains a critical challenge in orthopedic medicine. Extracellular matrix proteins play important roles in bionic mineralization and osteogenesis. Sacchachitin nanofibers (SCNFs) oxidized via TEMPO have shown great potential as biomaterial scaffolds due to the introduction of carboxylic groups that mimic the natural extracellular matrix. However, TEMPO is harmful to the environment, which calls for greener and sustainable alternatives. Therefore, this study explored the green fabrication of oxidized-SCNFs through SiliaCat-TEMPO (STEMPO) as a renewable catalyst with various amount of oxidation agents to evaluate their bone regeneration ability. We developed an optimal STEMPO oxidation process, enabling efficient removal of most STEMPO particles through centrifugation with minimal residue. The oxidation efficiency of recovered STEMPO particles remained comparable to fresh ones. STEMPO-oxidized SCNFs, oxidized at increasing NaClO levels, showed an increase in carboxylate content, from 0.1360 mmol/g to 3.2415 mmol/g. Additionally, STEMPO-oxidized SCNFs exhibited excellent biocompatibility with MC3T3-E1 osteoblastic cells, facilitating calcium precipitation, cell migration, and osteogenic differentiation. Mixed composites of β-tricalcium phosphate (BCP) and STEMPO-oxidized SCNFs with higher oxidation levels further enhanced osteogenic differentiation. In vivo studies using a rat femur defect model showed that SCNFs at the highest oxidation level (ST100SC) significantly promoted bone regeneration to full recovery, achieving a bone volume to total tissue volume (BV/TV) ratio of 75.6 %, compared to 48.3 % in the control. These results demonstrated that STEMPO-oxidized SCNFs with higher oxidation levels can be used as a bioactive matrix scaffold to achieve full recovery of bone regeneration.

骨再生仍然是骨科医学面临的一项严峻挑战。细胞外基质蛋白在仿生矿化和成骨过程中发挥着重要作用。通过 TEMPO 氧化的 Sacchachitin 纳米纤维（SCNFs）由于引入了模拟天然细胞外基质的羧基而显示出作为生物材料支架的巨大潜力。然而，TEMPO 对环境有害，因此需要更环保、更可持续的替代品。因此，本研究通过 SiliaCat-TEMPO（SiliaCat-TEMPO，STEMPO）作为可再生催化剂，并加入不同量的氧化剂，探索了氧化-SCNFs 的绿色制备方法，以评估其骨再生能力。我们开发了一种最佳 STEMPO 氧化工艺，可通过离心有效去除大部分 STEMPO 颗粒，残留物极少。回收的 STEMPO 粒子的氧化效率与新鲜粒子相当。STEMPO 氧化的 SCNFs 在 NaClO 含量增加的情况下，羧酸含量从 0.1360 mmol/g 增加到 3.2415 mmol/g。此外，STEMBO 氧化的 SCNF 与 MC3T3-E1 成骨细胞具有良好的生物相容性，可促进钙沉淀、细胞迁移和成骨分化。氧化程度较高的β-磷酸三钙（BCP）和 STEMPO 氧化 SCNF 的混合复合材料进一步促进了成骨分化。使用大鼠股骨缺损模型进行的体内研究表明，最高氧化水平（ST100SC）的 SCNFs 能显著促进骨再生，使骨完全恢复，骨体积与组织总体积（BV/TV）之比达到 75.6%，而对照组为 48.3%。这些结果表明，氧化程度较高的 STEMPO 氧化 SCNFs 可用作生物活性基质支架，实现骨再生的完全恢复。

{"title":"Bioactive matrix scaffold of oxidized sacchachitin via green catalyst of siliacat tempo for enhancing bone regeneration","authors":"Ming-Thau Sheu , Shyr-Yi Lin , Wei-Jie Cheng , Hong-Liang Lin , Chien-Ming Hsieh , Hsiu-O Ho , Ling-Chun Chen , Meng-Huang Wu","doi":"10.1016/j.carpta.2024.100576","DOIUrl":"10.1016/j.carpta.2024.100576","url":null,"abstract":"<div><div>Bone regeneration remains a critical challenge in orthopedic medicine. Extracellular matrix proteins play important roles in bionic mineralization and osteogenesis. Sacchachitin nanofibers (SCNFs) oxidized via TEMPO have shown great potential as biomaterial scaffolds due to the introduction of carboxylic groups that mimic the natural extracellular matrix. However, TEMPO is harmful to the environment, which calls for greener and sustainable alternatives. Therefore, this study explored the green fabrication of oxidized-SCNFs through SiliaCat-TEMPO (STEMPO) as a renewable catalyst with various amount of oxidation agents to evaluate their bone regeneration ability. We developed an optimal STEMPO oxidation process, enabling efficient removal of most STEMPO particles through centrifugation with minimal residue. The oxidation efficiency of recovered STEMPO particles remained comparable to fresh ones. STEMPO-oxidized SCNFs, oxidized at increasing NaClO levels, showed an increase in carboxylate content, from 0.1360 mmol/g to 3.2415 mmol/g. Additionally, STEMPO-oxidized SCNFs exhibited excellent biocompatibility with MC3T3-E1 osteoblastic cells, facilitating calcium precipitation, cell migration, and osteogenic differentiation. Mixed composites of β-tricalcium phosphate (BCP) and STEMPO-oxidized SCNFs with higher oxidation levels further enhanced osteogenic differentiation. <em>In vivo</em> studies using a rat femur defect model showed that SCNFs at the highest oxidation level (ST100SC) significantly promoted bone regeneration to full recovery, achieving a bone volume to total tissue volume (BV/TV) ratio of 75.6 %, compared to 48.3 % in the control. These results demonstrated that STEMPO-oxidized SCNFs with higher oxidation levels can be used as a bioactive matrix scaffold to achieve full recovery of bone regeneration.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100576"},"PeriodicalIF":6.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness evaluation of hyaluronic acid-based commercial eye drops to treat ophthalmic dry eye disease 基于透明质酸的商用滴眼液治疗眼科干眼症的效果评估

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-09-30 DOI: 10.1016/j.carpta.2024.100577

Jon Andrade del Olmo, Virginia Sáez Martínez, Nagore Martínez de Cestafe, José María Alonso, Cristina Olavarrieta, Miguel Ucelay López de Heredia, Sandra Benito Cid, Raúl Pérez González

Nowadays, hyaluronic acid (HA) eye drops are widely used to combat ophthalmic dry eye disease (DED). Till the date, almost all commercialized HA eye drops are single-ingredient despite not displaying significantly different clinical effects (Artelac Splash®). Consequently, scientists are making great efforts to combine HA with other compounds to overcome more successfully DED (trometamol in Hyabak®, sorbitol in HyloComod®). Gamma-polyglutamic acid (γ-PGA) is another novel polymer that could induce a powerful synergistic effect on HA for DED treatment. To the best of our knowledge, eye drops combining HA and γ-PGA neither have been marketed nor scientific studies have been published so far. Fortunately, DayDrop Advanced® eye drop has been recently commercialized with HA + γ-PGA technology. In this work, DayDrop Advanced®, Hyabak®, HyloComod® and Artelac Splash® were compared evaluating their efficacy to treat DED and residence time in eye due to their distinct physicochemical and rheological properties. As key research point, DayDrop Advanced® demonstrated an improved immediate hydration, protection against corneal dehydration, anti-inflammatory, anti-hyaluronidase and ocular mucoadhesion activities than the rest of artificial tears. These phenomena highlight new generation DayDrop Advanced® combining HA + γ-PGA technology as the leading eye drops to treat more durably and effectively DED.

如今，透明质酸（HA）滴眼液被广泛用于防治眼科干眼症（DED）。迄今为止，几乎所有商品化的透明质酸滴眼液都是单一成分的，尽管其临床效果并无明显差异（Artelac Splash®）。因此，科学家们正努力将 HA 与其他化合物结合起来，以更成功地克服 DED（Hyabak® 中的曲美他莫、HyloComod® 中的山梨醇）。γ-聚谷氨酸（γ-PGA）是另一种新型聚合物，可在治疗 DED 时与 HA 产生强大的协同效应。据我们所知，结合了 HA 和 γ-PGA 的滴眼液至今既未在市场上销售，也未发表过科学研究报告。幸运的是，采用 HA + γ-PGA 技术的 DayDrop Advanced® 滴眼液已于近期上市。在这项研究中，对 DayDrop Advanced®、Hyabak®、HyloComod® 和 Artelac Splash® 进行了比较，评估了它们治疗 DED 的疗效以及因其不同的理化和流变特性而导致的在眼内的停留时间。作为研究的关键点，与其他人工泪液相比，DayDrop Advanced® 在即时水合、防止角膜脱水、抗炎、抗透明质酸酶和眼部粘附等方面都有更好的表现。这些现象凸显了结合 HA + γ-PGA 技术的新一代 DayDrop Advanced® 是更持久、更有效治疗 DED 的领先滴眼液。

{"title":"Effectiveness evaluation of hyaluronic acid-based commercial eye drops to treat ophthalmic dry eye disease","authors":"Jon Andrade del Olmo, Virginia Sáez Martínez, Nagore Martínez de Cestafe, José María Alonso, Cristina Olavarrieta, Miguel Ucelay López de Heredia, Sandra Benito Cid, Raúl Pérez González","doi":"10.1016/j.carpta.2024.100577","DOIUrl":"10.1016/j.carpta.2024.100577","url":null,"abstract":"<div><div>Nowadays, hyaluronic acid (HA) eye drops are widely used to combat ophthalmic dry eye disease (DED). Till the date, almost all commercialized HA eye drops are single-ingredient despite not displaying significantly different clinical effects (Artelac Splash®). Consequently, scientists are making great efforts to combine HA with other compounds to overcome more successfully DED (trometamol in Hyabak®, sorbitol in HyloComod®). Gamma-polyglutamic acid (γ-PGA) is another novel polymer that could induce a powerful synergistic effect on HA for DED treatment. To the best of our knowledge, eye drops combining HA and γ-PGA neither have been marketed nor scientific studies have been published so far. Fortunately, DayDrop Advanced® eye drop has been recently commercialized with HA + γ-PGA technology. In this work, DayDrop Advanced®, Hyabak®, HyloComod® and Artelac Splash® were compared evaluating their efficacy to treat DED and residence time in eye due to their distinct physicochemical and rheological properties. As key research point, DayDrop Advanced® demonstrated an improved immediate hydration, protection against corneal dehydration, anti-inflammatory, anti-hyaluronidase and ocular mucoadhesion activities than the rest of artificial tears. These phenomena highlight new generation DayDrop Advanced® combining HA + γ-PGA technology as the leading eye drops to treat more durably and effectively DED.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100577"},"PeriodicalIF":6.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142418590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing emulsion stability and adsorption of Pickering emulsions using alkylated cellulose nanofibers 使用烷基化纤维素纳米纤维增强皮克林乳液的稳定性和吸附性

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-09-25 DOI: 10.1016/j.carpta.2024.100574

Miyu Tanzawa , Noriko Kanai , Takahiro Sakai , Kohei Yamada , Sari Kumagai , Batsaikhan Mijiddorj , Izuru Kawamura

Cellulose nanofibers (CNFs) extracted from plant cell walls form a three-dimensional network in water and stabilize the dispersion of droplets, which are used as emulsion stabilizers. In this study, four types of alkyl-grafted CNFs (ACNFs) with different lengths of dialkyl chains (diC4, diC6, diC8, and diC10) were synthesized by the surface modification of 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-oxidized CNF (TOCNF) to improve the stability of oil-in-water Pickering emulsions (PEs). The ACNF-stabilized emulsions with a 20/80 ratio of oil/water were prepared using two different oils (olive oil and eucalyptus oil) and the ACNF 1.0 % dispersion, and the changes over time were investigated at approximately 25 °C. Compared to TOCNF, the ACNFs provided more stable olive oil-PE (o-PE) and eucalyptus oil-PE (e-PE) although the stabilization behaviors of o-PE and e-PE differed. Olive oil, which contained rich long-chain fatty acids, was more likely to interact with the ACNFs, thereby promoting the interfacial adsorption of oil droplets. On the other hand, eucalyptus oil is mainly composed of 1,8-cineole and does not have alkyl chains, so the interaction with the ACNFs was weak and therefore, sufficient interfacial adsorption could not be obtained. The results of the particle size distribution and viscosity measurements also showed that the ACNFs contributed significantly to the stabilization of o-PE. Based on the overall experimental results, it was determined that the diC6-ACNF gave the most significant stability improvement. Therefore, we designed an alkylated CNF that mimics diC6-ACNF for MD simulations. MD simulations showed that hydrophobic modification created a hydrophobic interaction between the alkyl chain and dodecane molecules as the oil, leading to the enhancing adsorption of ACNF at the oil droplet interfaces.

从植物细胞壁中提取的纤维素纳米纤维（CNFs）可在水中形成三维网络，稳定液滴的分散，可用作乳液稳定剂。本研究通过对 2,2,6,6-四甲基哌啶-1-氧自由基（TEMPO）氧化的 CNF（TOCNF）进行表面改性，合成了四种具有不同长度二烷基链（diC4、diC6、diC8 和 diC10）的烷基接枝 CNF（ACNF），以提高水包油型皮克林乳液（PE）的稳定性。使用两种不同的油（橄榄油和桉树油）和 ACNF 1.0 % 分散液制备了油水比为 20/80 的 ACNF 稳定乳液，并在约 25 °C 的温度下研究了其随时间的变化。与 TOCNF 相比，ACNF 提供了更稳定的橄榄油-聚乙烯（o-PE）和桉树油-聚乙烯（e-PE），尽管 o-PE 和 e-PE 的稳定行为有所不同。橄榄油含有丰富的长链脂肪酸，更容易与 ACNFs 发生相互作用，从而促进油滴的界面吸附。另一方面，桉树油的主要成分是 1,8-蒎烯，不含烷基链，因此与 ACNF 的相互作用较弱，无法获得充分的界面吸附。粒度分布和粘度测量的结果也表明，ACNFs 对邻丙醚的稳定作用很大。根据总体实验结果，我们确定 diC6-ACNF 对稳定性的改善最为显著。因此，我们设计了一种模仿 diC6-ACNF 的烷基化 CNF 进行 MD 模拟。MD 模拟结果表明，疏水改性在烷基链和作为油的十二烷分子之间产生了疏水相互作用，从而增强了 ACNF 在油滴界面的吸附性。

{"title":"Enhancing emulsion stability and adsorption of Pickering emulsions using alkylated cellulose nanofibers","authors":"Miyu Tanzawa , Noriko Kanai , Takahiro Sakai , Kohei Yamada , Sari Kumagai , Batsaikhan Mijiddorj , Izuru Kawamura","doi":"10.1016/j.carpta.2024.100574","DOIUrl":"10.1016/j.carpta.2024.100574","url":null,"abstract":"<div><div>Cellulose nanofibers (CNFs) extracted from plant cell walls form a three-dimensional network in water and stabilize the dispersion of droplets, which are used as emulsion stabilizers. In this study, four types of alkyl-grafted CNFs (ACNFs) with different lengths of dialkyl chains (diC4, diC6, diC8, and diC10) were synthesized by the surface modification of 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-oxidized CNF (TOCNF) to improve the stability of oil-in-water Pickering emulsions (PEs). The ACNF-stabilized emulsions with a 20/80 ratio of oil/water were prepared using two different oils (olive oil and eucalyptus oil) and the ACNF 1.0 % dispersion, and the changes over time were investigated at approximately 25 °C. Compared to TOCNF, the ACNFs provided more stable olive oil-PE (o-PE) and eucalyptus oil-PE (e-PE) although the stabilization behaviors of o-PE and e-PE differed. Olive oil, which contained rich long-chain fatty acids, was more likely to interact with the ACNFs, thereby promoting the interfacial adsorption of oil droplets. On the other hand, eucalyptus oil is mainly composed of 1,8-cineole and does not have alkyl chains, so the interaction with the ACNFs was weak and therefore, sufficient interfacial adsorption could not be obtained. The results of the particle size distribution and viscosity measurements also showed that the ACNFs contributed significantly to the stabilization of o-PE. Based on the overall experimental results, it was determined that the diC6-ACNF gave the most significant stability improvement. Therefore, we designed an alkylated CNF that mimics diC6-ACNF for MD simulations. MD simulations showed that hydrophobic modification created a hydrophobic interaction between the alkyl chain and dodecane molecules as the oil, leading to the enhancing adsorption of ACNF at the oil droplet interfaces.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100574"},"PeriodicalIF":6.2,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gelatin-based ballistic gel formulated with phytosynthesized nanocellulose from Arundo donax for alpha-amylase enzyme inhibition activity 明胶基弹道凝胶与植物合成纳米纤维素配制而成，具有抑制α-淀粉酶的活性

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-09-24 DOI: 10.1016/j.carpta.2024.100575

Jaison Jeevanandam, Rita Castro, João Rodrigues

Arundo donax, an indigenous plant in the Mediterranean region, has affected the growth of native vegetation as an invasive plant, ultimately reducing soil quality and affecting the food chain in several areas of the world. Thus, the present work aims to obtain cellulose from the A. donax leaf extract via organosolv fractionation and bleaching method, followed by centrifugal fractionation to yield nanocellulose of moderately monodispersed (0.428 PDI) 91.2 nm in size and a zeta potential of -35.5 mV of high stability in aqueous medium (water). In addition, the resultant nanocellulose was formulated in ballistic gelatin hydrogel, and their antidiabetic effect via alpha-amylase enzyme inhibition activity was also investigated. Systematic characterization of the hydrogel samples (different volume ratios of gelatin and nanocellulose) revealed that the sample with 18:2 (v/v) gelatin:nanocellulose content is thermally stable until 152 °C, releasing nanocellulose for three days from hydrogel until 45 °C. This study confirms the feasibility and potential of utilizing invasive plant species to extract nanocellulose for medical purposes. Furthermore, the gelatin-formulated nanocellulose, with its demonstrated alpha-amylase inhibition activity at a dosage of 80 µg/mL, holds the promise of being a potential revolutionary medical gummy for sustained reduction of blood glucose levels.

唐蜡（Arundo donax）是地中海地区的一种本地植物，作为一种入侵植物，它影响了本地植被的生长，最终降低了土壤质量，并影响了世界多个地区的食物链。因此，本研究旨在通过有机溶胶分馏和漂白法从唐蜡叶提取物中获得纤维素，然后进行离心分馏，得到中等单分散（0.428 PDI）的纳米纤维素，粒径为 91.2 nm，Zeta 电位为 -35.5 mV，在水介质（水）中具有高稳定性。此外，还将制得的纳米纤维素配制成弹道明胶水凝胶，并通过α-淀粉酶抑制活性研究了其抗糖尿病效果。对水凝胶样品（明胶和纳米纤维素的不同体积比）进行的系统表征表明，明胶与纳米纤维素含量为 18:2 (v/v) 的样品在 152 °C 前具有热稳定性，在 45 °C 前，纳米纤维素可从水凝胶中释放三天。这项研究证实了利用入侵植物物种提取纳米纤维素用于医疗目的的可行性和潜力。此外，明胶配制的纳米纤维素在剂量为 80 µg/mL 时具有抑制α-淀粉酶的活性，有望成为一种潜在的革命性医疗软糖，用于持续降低血糖水平。

{"title":"Gelatin-based ballistic gel formulated with phytosynthesized nanocellulose from Arundo donax for alpha-amylase enzyme inhibition activity","authors":"Jaison Jeevanandam, Rita Castro, João Rodrigues","doi":"10.1016/j.carpta.2024.100575","DOIUrl":"10.1016/j.carpta.2024.100575","url":null,"abstract":"<div><div><em>Arundo donax</em>, an indigenous plant in the Mediterranean region, has affected the growth of native vegetation as an invasive plant, ultimately reducing soil quality and affecting the food chain in several areas of the world. Thus, the present work aims to obtain cellulose from the <em>A. donax</em> leaf extract via organosolv fractionation and bleaching method, followed by centrifugal fractionation to yield nanocellulose of moderately monodispersed (0.428 PDI) 91.2 nm in size and a zeta potential of -35.5 mV of high stability in aqueous medium (water). In addition, the resultant nanocellulose was formulated in ballistic gelatin hydrogel, and their antidiabetic effect via alpha-amylase enzyme inhibition activity was also investigated. Systematic characterization of the hydrogel samples (different volume ratios of gelatin and nanocellulose) revealed that the sample with 18:2 (v/v) gelatin:nanocellulose content is thermally stable until 152 °C, releasing nanocellulose for three days from hydrogel until 45 °C. This study confirms the feasibility and potential of utilizing invasive plant species to extract nanocellulose for medical purposes. Furthermore, the gelatin-formulated nanocellulose, with its demonstrated alpha-amylase inhibition activity at a dosage of 80 µg/mL, holds the promise of being a potential revolutionary medical gummy for sustained reduction of blood glucose levels.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100575"},"PeriodicalIF":6.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functionalized cellulose nanofiber films as potential substitutes for Japanese paper 作为日本纸潜在替代品的功能化纤维素纳米纤维薄膜

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-09-24 DOI: 10.1016/j.carpta.2024.100573

Ricardo Almeida , Ana Ramos , Verner Håkonsen , Thaddeus Maloney , José Gamelas

Japanese papers (JPs) are among the most common materials used for the restoration of historical papers. However, they have low transparency and may structure the paper surface. To overcome the limitations of JPs, in the present work films of cellulose nanofibers with and without lignin (LCNFs and CNFs, respectively) were explored. For this, chemically different LCNFs (carboxylated by TEMPO-mediated oxidation using variable oxidant amount and sulfated with deep eutectic solvent) and two reference CNFs were produced from Eucalyptus kraft pulps. Lignin slightly hindered the introduction of carboxyl groups into the cellulose structure, but did not affect the incorporation of sulfate groups. (L)CNFs gave films with better mechanical properties than those determined for three types of JP (tensile strength of 79–142 MPa (±11) vs 3.5–13.0 MPa (±0.4) and Young's modulus of 4900–5900 MPa (±300) vs 240–1800 MPa (±70)). Additionally, the (L)CNF films exhibited lower water vapor transmission rates (132–312 g.m⁻².day⁻¹) compared to the JPs (685–719 g.m⁻².day⁻¹), and a higher transparency (up to 89% and ≤82% for JPs). Except for the thermal stability, all properties studied favored (L)CNF films over JPs, indicating their strong potential for restoration/conservation applications. This systematic comparison between the properties of (L)CNF films and JPs has never been conducted before.

日本纸（JP）是修复历史纸张最常用的材料之一。然而，它们的透明度较低，而且可能会使纸张表面产生结构。为了克服日本纸的局限性，本研究探讨了含木质素和不含木质素的纤维素纳米纤维薄膜（分别为 LCNFs 和 CNFs）。为此，我们从桉树牛皮浆中制备了化学性质不同的 LCNFs（通过 TEMPO 介导的氧化作用，使用不同量的氧化剂进行羧化，并使用深共晶溶剂进行硫化）和两种参考 CNFs。木质素略微阻碍了纤维素结构中羧基的引入，但并不影响硫酸基团的加入。(与三种 JP 相比，(L)CNF 所制成的薄膜具有更好的机械性能（拉伸强度为 79-142 兆帕（±11）比 3.5-13.0 兆帕（±0.4），杨氏模量为 4900-5900 兆帕（±300）比 240-1800 兆帕（±70））。此外，(L)CNF 薄膜的水蒸气透过率（132-312 g.m-2.day-1）低于日本涂料（685-719 g.m-2.day-1），透明度更高（日本涂料高达 89%，而≤82%）。除热稳定性外，所研究的所有特性都表明，(L)CNF 薄膜都优于 JP，这表明它们在修复/保护应用方面具有很大的潜力。这种对（L）CNF 薄膜和 JP 的性能进行系统比较的做法以前从未有过。

{"title":"Functionalized cellulose nanofiber films as potential substitutes for Japanese paper","authors":"Ricardo Almeida , Ana Ramos , Verner Håkonsen , Thaddeus Maloney , José Gamelas","doi":"10.1016/j.carpta.2024.100573","DOIUrl":"10.1016/j.carpta.2024.100573","url":null,"abstract":"<div><div>Japanese papers (JPs) are among the most common materials used for the restoration of historical papers. However, they have low transparency and may structure the paper surface. To overcome the limitations of JPs, in the present work films of cellulose nanofibers with and without lignin (LCNFs and CNFs, respectively) were explored. For this, chemically different LCNFs (carboxylated by TEMPO-mediated oxidation using variable oxidant amount and sulfated with deep eutectic solvent) and two reference CNFs were produced from <em>Eucalyptus</em> kraft pulps. Lignin slightly hindered the introduction of carboxyl groups into the cellulose structure, but did not affect the incorporation of sulfate groups. (L)CNFs gave films with better mechanical properties than those determined for three types of JP (tensile strength of 79–142 MPa (±11) vs 3.5–13.0 MPa (±0.4) and Young's modulus of 4900–5900 MPa (±300) vs 240–1800 MPa (±70)). Additionally, the (L)CNF films exhibited lower water vapor transmission rates (132–312 g.m<sup>−2</sup>.day<sup>−1</sup>) compared to the JPs (685–719 g.m<sup>−2</sup>.day<sup>−1</sup>), and a higher transparency (up to 89% and ≤82% for JPs). Except for the thermal stability, all properties studied favored (L)CNF films over JPs, indicating their strong potential for restoration/conservation applications. This systematic comparison between the properties of (L)CNF films and JPs has never been conducted before.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100573"},"PeriodicalIF":6.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice 细菌协同发酵介导的蝲蛄壳聚糖合成可增强水稻的抗病性

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2024-09-21 DOI: 10.1016/j.carpta.2024.100571

Lirong Wei , Yiqiong Sun , Yingqiao Wan , Shangyu Gao , Sun Tae Kim , Ravi Gupta , Yiming Wang

The increasing dietary demand for red swamp crayfish (Procambarus clarkii) in China has resulted in a significant rise in the quantity of its by-products, which are currently underutilized or discarded. Chitosan, a widely used plant immune inducer, is one of the major components found in red swamp crayfish shells (RSCS). However, the utilization of chitosan derived from RSCS in agriculture is hindered by the lack of an efficient microbial fermentation-based production system. To address this issue, this study aims to establish a chitosan fermentation procedure by using RSCS as substrate. The efficiency by using Bacillus followed with Acetobacter for a continuous co-fermentation tested. Bacillus NJ-B2 or NJ-B3 with Acetobacter NJ-A1 demonstrated the highest co-fermentation efficacy, achieving 95 % deproteinization and 90 % demineralization of RSCS, respectively. The extracted chitosan was characterized by fourier transform infrared and scanning electron microscopy. Notably, the application of this RSCS-derived chitosan significantly improves rice resistance against Xanthomonas oryzae pv. oryzae, Magnaporthe oryzae, and Ustilaginoidea virens. These findings collectively indicate that this established method can effectively produce chitosan from RSCS via microbial fermentation, providing an environmentally friendly way for plant protection.

中国人对红沼泽小龙虾（Procambarus clarkii）的饮食需求不断增加，导致其副产品的数量大幅上升，而这些副产品目前尚未得到充分利用或被丢弃。甲壳素是一种广泛使用的植物免疫诱导剂，也是红沼泽小龙虾壳（RSCS）中的主要成分之一。然而，由于缺乏高效的微生物发酵生产系统，从红沼泽螯虾壳中提取的壳聚糖在农业中的应用受到阻碍。为解决这一问题，本研究旨在建立一种以 RSCS 为底物的壳聚糖发酵程序。测试了使用芽孢杆菌和醋酸纤维菌进行连续共发酵的效率。芽孢杆菌 NJ-B2 或 NJ-B3 与醋酸纤维菌 NJ-A1 共同发酵的效率最高，分别实现了 RSCS 95% 的脱蛋白和 90% 的脱矿物质。傅立叶变换红外光谱和扫描电子显微镜对提取的壳聚糖进行了表征。值得注意的是，应用这种从 RSCS 提取的壳聚糖可显著提高水稻对黄单胞菌（Xanthomonas oryzae pv. oryzae）、木格氏球菌（Magnaporthe oryzae）和 Ustilaginoidea virens 的抗性。这些研究结果共同表明，这种成熟的方法可以通过微生物发酵从 RSCS 中有效地生产壳聚糖，为植物保护提供了一种环境友好型方法。

{"title":"Bacterial co-fermentation mediated synthesis of chitosan from Procambarus clarkii enhances disease resistance in rice","authors":"Lirong Wei , Yiqiong Sun , Yingqiao Wan , Shangyu Gao , Sun Tae Kim , Ravi Gupta , Yiming Wang","doi":"10.1016/j.carpta.2024.100571","DOIUrl":"10.1016/j.carpta.2024.100571","url":null,"abstract":"<div><div>The increasing dietary demand for red swamp crayfish (<em>Procambarus clarkii</em>) in China has resulted in a significant rise in the quantity of its by-products, which are currently underutilized or discarded. Chitosan, a widely used plant immune inducer, is one of the major components found in red swamp crayfish shells (RSCS). However, the utilization of chitosan derived from RSCS in agriculture is hindered by the lack of an efficient microbial fermentation-based production system. To address this issue, this study aims to establish a chitosan fermentation procedure by using RSCS as substrate. The efficiency by using <em>Bacillus</em> followed with <em>Acetobacter</em> for a continuous co-fermentation tested. <em>Bacillus</em> NJ-B2 or NJ-B3 with <em>Acetobacter</em> NJ-A1 demonstrated the highest co-fermentation efficacy, achieving 95 % deproteinization and 90 % demineralization of RSCS, respectively. The extracted chitosan was characterized by fourier transform infrared and scanning electron microscopy. Notably, the application of this RSCS-derived chitosan significantly improves rice resistance against <em>Xanthomonas oryzae</em> pv. <em>oryzae, Magnaporthe oryzae</em>, and <em>Ustilaginoidea virens</em>. These findings collectively indicate that this established method can effectively produce chitosan from RSCS via microbial fermentation, providing an environmentally friendly way for plant protection.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"8 ","pages":"Article 100571"},"PeriodicalIF":6.2,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0