Pub Date : 2022-04-01DOI: 10.12982/cmujns.2022.026
Saranya Wannachaiyakul, A. Klunklin, N. Sansiriphun, Jirawan Deeluea, S. Niyomkar, W. Kunaviktikul, Prachayaporn Charoenpakdee
Abstract Music is an evidence-based, complementary therapy used as a holistic approach for enhancing the quality of life. The music focuses on all levels of consciousness and promotes healing through sound and physical response which can reduce anxiety, resulting in relaxation. This study aimed to investigate the effectiveness of Lanna music for relaxation and reducing stress. A randomized controlled trial enrolled 52 persons with mild to moderate stress and/or depression. Participants were randomly assigned to either the experimental group, who listened to Lanna music for 60 minutes, or the control group, who were instructed to lie down in a comfortable position without the Lanna music. In addition, demographic data, blood pressure, heart rate, respiration rate, alpha brain wave frequency and the responses to the relaxation visual analog rating scale were collected. Descriptive statistics, a repeated measures ANOVA, a paired t-test and an independent t-test were used to test the effects of Lanna music in reducing stress. The analysis showed a significant reduction (P < .05) in the mean score of heart rate, respiration rate, and systolic and diastolic blood pressure (P < .05) after listening to the traditional Lanna music. Additionally, there was no significant increase in the mean score of the alpha brain wave after 30 minutes. The study results showed that Lanna music for relaxation had an overall significant effect on stress reduction. Listening to Lanna music can reduce heart rate, respiration rate, and blood pressure, especially increasing the alpha brain waves which has the clinical impact of stress reduction and relaxation responses. These findings indicated the positive effect of traditional Lanna music for relaxation and reducing stress. Keywords: Randomized controlled trial, Lanna music, Stress
{"title":"Effects of Lanna Music for Relaxation on Reducing Stress in Adults: A Randomized Controlled Trial","authors":"Saranya Wannachaiyakul, A. Klunklin, N. Sansiriphun, Jirawan Deeluea, S. Niyomkar, W. Kunaviktikul, Prachayaporn Charoenpakdee","doi":"10.12982/cmujns.2022.026","DOIUrl":"https://doi.org/10.12982/cmujns.2022.026","url":null,"abstract":"Abstract Music is an evidence-based, complementary therapy used as a holistic approach for enhancing the quality of life. The music focuses on all levels of consciousness and promotes healing through sound and physical response which can reduce anxiety, resulting in relaxation. This study aimed to investigate the effectiveness of Lanna music for relaxation and reducing stress. A randomized controlled trial enrolled 52 persons with mild to moderate stress and/or depression. Participants were randomly assigned to either the experimental group, who listened to Lanna music for 60 minutes, or the control group, who were instructed to lie down in a comfortable position without the Lanna music. In addition, demographic data, blood pressure, heart rate, respiration rate, alpha brain wave frequency and the responses to the relaxation visual analog rating scale were collected. Descriptive statistics, a repeated measures ANOVA, a paired t-test and an independent t-test were used to test the effects of Lanna music in reducing stress. The analysis showed a significant reduction (P < .05) in the mean score of heart rate, respiration rate, and systolic and diastolic blood pressure (P < .05) after listening to the traditional Lanna music. Additionally, there was no significant increase in the mean score of the alpha brain wave after 30 minutes. The study results showed that Lanna music for relaxation had an overall significant effect on stress reduction. Listening to Lanna music can reduce heart rate, respiration rate, and blood pressure, especially increasing the alpha brain waves which has the clinical impact of stress reduction and relaxation responses. These findings indicated the positive effect of traditional Lanna music for relaxation and reducing stress. Keywords: Randomized controlled trial, Lanna music, Stress","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45215591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.12982/cmujns.2022.021
N. Kristianto, M. Sari, Y. Yusuf
Abstract In this study, hydroxyapatite (HA) from abalone mussel shells (Haliotis asinina) is synthesized using the precipitation method with a stirring time of 30 min and a calcination temperature of 1000°C. HA was used for coating Ti alloy using the electrophoretic deposition dip coating (EP2D) method. The coating applied three variations, including the DC voltages variation of 25 V and 50 V, the withdrawal speeds of 0.1 mm/s, 0.5 mm/s, 1 mm/s, and the calcination temperatures of 750°C and 950°C. Energy dispersive X-Ray spectroscopy (EDS) revealed the Ca/P molar ratio of HA was 1.63, which is close to the stoichiometric ratio of HA at 1.67. The distance between the crystal planes of the HA was 2.81Å. This result also is relative to the crystal plane of the HA at 2.88Å. The EP2D process and the calcination temperature treatment will remove the B-type carbonate apatite phase, so the purity of the HA layer is higher. SEM results show that the HA layer formed was more homogeneous and thicker at the DC voltage of 50 V and the withdrawal velocity of 0.1 mm/s. At this voltage and velocity, minor cracking and agglomeration were produced. The density of the HA layer was higher with increasing calcination temperature and DC voltages. Keywords: Hydroxyapatite, Titanium Alloy, Coating, Electrophoretic Deposition Dip Coating
{"title":"Hydroxyapatite based on Abalone Mussel Shells Coating on Titanium Alloy using Electrophoretic Deposition Dip Coating as a Bone Implant Candidate","authors":"N. Kristianto, M. Sari, Y. Yusuf","doi":"10.12982/cmujns.2022.021","DOIUrl":"https://doi.org/10.12982/cmujns.2022.021","url":null,"abstract":"Abstract In this study, hydroxyapatite (HA) from abalone mussel shells (Haliotis asinina) is synthesized using the precipitation method with a stirring time of 30 min and a calcination temperature of 1000°C. HA was used for coating Ti alloy using the electrophoretic deposition dip coating (EP2D) method. The coating applied three variations, including the DC voltages variation of 25 V and 50 V, the withdrawal speeds of 0.1 mm/s, 0.5 mm/s, 1 mm/s, and the calcination temperatures of 750°C and 950°C. Energy dispersive X-Ray spectroscopy (EDS) revealed the Ca/P molar ratio of HA was 1.63, which is close to the stoichiometric ratio of HA at 1.67. The distance between the crystal planes of the HA was 2.81Å. This result also is relative to the crystal plane of the HA at 2.88Å. The EP2D process and the calcination temperature treatment will remove the B-type carbonate apatite phase, so the purity of the HA layer is higher. SEM results show that the HA layer formed was more homogeneous and thicker at the DC voltage of 50 V and the withdrawal velocity of 0.1 mm/s. At this voltage and velocity, minor cracking and agglomeration were produced. The density of the HA layer was higher with increasing calcination temperature and DC voltages. Keywords: Hydroxyapatite, Titanium Alloy, Coating, Electrophoretic Deposition Dip Coating","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46021980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.12982/cmujns.2022.033
Muhamad Fauzi Antoni, P. S. Rejeki, S. _, A. Pranoto, Kristanti Wanito Wigati, G. Sari, R. Lesmana, Y. Yamaoka
Abstract This study aims to compare moderate-intensity swimming exercise in the morning and at night towards the increment of irisin levels in female mice (Mus musculus). This research is a real experiment with the randomized control group post-test-only design. A total of 24 female mice (Mus musculus), aged 3 months old, and weighing 20-40 grams (Lee’s Index Value > 0.3) were randomized into three groups, namely G1 (n = 8, control without intervention), G2 (n = 8, moderate-intensity swimming exercise in the morning), and G3 (n = 8, moderate-intensity swimming exercise at night). Swimming exercises were carried out 3x/w for 4 weeks with an intensity of 6% of body weight and the duration was 70% of maximum swimming time. Irisin levels were measured using the ELISA method. The data analysis techniques used were one-way ANOVA test and Tukey's HSD post-hoc test. The results that were obtained from this experimental study were mean levels of irisin in G1 (1.86 ± 0.06 ng/mL), G2 (2.66 ± 0.12 ng/mL), G3 (3.43 ± 0.35 ng/mL), and (P ≤ 0.001). The results of Tukey's HSD post-hoc test showed that there was a significant difference in the mean post-training irisin levels between G2 and G1 (P ≤ 0.05), G3 and G1 (P ≤ 0.001), and G3 and G2 (P ≤ 0.05). Based on the results of this study, it was concluded that moderate-intensity swimming exercises in the morning and at night were able to increase irisin levels. However, moderate-intensity swimming exercise at night showed a higher effectiveness in increasing irisin levels of female mice (Mus musculus). Keywords: Obesity, Irisin levels, Morning exercise, Night exercise
{"title":"Effect of Nocturnal and Diurnal Moderate-intensity Swimming Exercise on Increasing Irisin Level of Female Mice (Mus musculus)","authors":"Muhamad Fauzi Antoni, P. S. Rejeki, S. _, A. Pranoto, Kristanti Wanito Wigati, G. Sari, R. Lesmana, Y. Yamaoka","doi":"10.12982/cmujns.2022.033","DOIUrl":"https://doi.org/10.12982/cmujns.2022.033","url":null,"abstract":"Abstract This study aims to compare moderate-intensity swimming exercise in the morning and at night towards the increment of irisin levels in female mice (Mus musculus). This research is a real experiment with the randomized control group post-test-only design. A total of 24 female mice (Mus musculus), aged 3 months old, and weighing 20-40 grams (Lee’s Index Value > 0.3) were randomized into three groups, namely G1 (n = 8, control without intervention), G2 (n = 8, moderate-intensity swimming exercise in the morning), and G3 (n = 8, moderate-intensity swimming exercise at night). Swimming exercises were carried out 3x/w for 4 weeks with an intensity of 6% of body weight and the duration was 70% of maximum swimming time. Irisin levels were measured using the ELISA method. The data analysis techniques used were one-way ANOVA test and Tukey's HSD post-hoc test. The results that were obtained from this experimental study were mean levels of irisin in G1 (1.86 ± 0.06 ng/mL), G2 (2.66 ± 0.12 ng/mL), G3 (3.43 ± 0.35 ng/mL), and (P ≤ 0.001). The results of Tukey's HSD post-hoc test showed that there was a significant difference in the mean post-training irisin levels between G2 and G1 (P ≤ 0.05), G3 and G1 (P ≤ 0.001), and G3 and G2 (P ≤ 0.05). Based on the results of this study, it was concluded that moderate-intensity swimming exercises in the morning and at night were able to increase irisin levels. However, moderate-intensity swimming exercise at night showed a higher effectiveness in increasing irisin levels of female mice (Mus musculus). Keywords: Obesity, Irisin levels, Morning exercise, Night exercise","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43920759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.12982/cmujns.2022.031
A. Khangtragool, K. Sunkonkit, A. Lucksiri, S. Seetaboot
Abstract There is limited information available regarding clinical outcome and rate of mortality in relation to invasive methicillin-resistant Staphylococcus aureus (MRSA) infection in pediatric intensive care unit (PICU) patients treated with vancomycin in this tertiary hospital in northern Thailand. Therapeutic drug monitoring (TDM) is recommended vancomycin prescription; however, it is important to investigate the outcome of this monitoring in PICU patients. This study aims to evaluate the mortality and factors related to these in PICU patients treated with vancomycin. A retrospective study was conducted in PICU patients given vancomycin from April 2018 to April 2019. The following variables were included: age, sex, underlying disease, diagnosis, length of stay (LOS) in PICU, Pediatric Index of Mortality 2 (PIM 2) score, mechanical ventilator use, renal replacement therapy (RRT), laboratory data, vancomycin dose, trough serum vancomycin concentration (Ctrough) and mortality rate. One hundred and sixty pediatric patients were enrolled into the study (median age 12 months, range 2-180 months, 69.4% male). Ctrough of vancomycin (10–20 mg/L) was recorded in 32.5% (n = 52) of cases. Septic shock was the most common diagnosis (49.3%) and the mortality rate was 39.4%. Results indicated that children who had a vancomycin Ctrough outside the therapeutic range, mechanical ventilator use and RRT use were statistically significantly associated with higher mortality rate (adjusted OR 3.29, 95% CI, 1.41-7.69; P <0.05), (adjusted OR 6.22, 95% CI, 1.67-23.16; P < 0.05) and (adjusted OR 10.41, 95% CI, 2.62-41.37; P < 0.05). These factors were related to mortality and further studies are needed to determine if this outcome can be improved. Keywords: Vancomycin, Vancomycin trough concentration, Pediatric patients, Intensive care, Mortality, Factors related to mortality
{"title":"Mortality and Factors Related in Pediatric Intensive Care Unit Patients Treated with Vancomycin","authors":"A. Khangtragool, K. Sunkonkit, A. Lucksiri, S. Seetaboot","doi":"10.12982/cmujns.2022.031","DOIUrl":"https://doi.org/10.12982/cmujns.2022.031","url":null,"abstract":"Abstract There is limited information available regarding clinical outcome and rate of mortality in relation to invasive methicillin-resistant Staphylococcus aureus (MRSA) infection in pediatric intensive care unit (PICU) patients treated with vancomycin in this tertiary hospital in northern Thailand. Therapeutic drug monitoring (TDM) is recommended vancomycin prescription; however, it is important to investigate the outcome of this monitoring in PICU patients. This study aims to evaluate the mortality and factors related to these in PICU patients treated with vancomycin. A retrospective study was conducted in PICU patients given vancomycin from April 2018 to April 2019. The following variables were included: age, sex, underlying disease, diagnosis, length of stay (LOS) in PICU, Pediatric Index of Mortality 2 (PIM 2) score, mechanical ventilator use, renal replacement therapy (RRT), laboratory data, vancomycin dose, trough serum vancomycin concentration (Ctrough) and mortality rate. One hundred and sixty pediatric patients were enrolled into the study (median age 12 months, range 2-180 months, 69.4% male). Ctrough of vancomycin (10–20 mg/L) was recorded in 32.5% (n = 52) of cases. Septic shock was the most common diagnosis (49.3%) and the mortality rate was 39.4%. Results indicated that children who had a vancomycin Ctrough outside the therapeutic range, mechanical ventilator use and RRT use were statistically significantly associated with higher mortality rate (adjusted OR 3.29, 95% CI, 1.41-7.69; P <0.05), (adjusted OR 6.22, 95% CI, 1.67-23.16; P < 0.05) and (adjusted OR 10.41, 95% CI, 2.62-41.37; P < 0.05). These factors were related to mortality and further studies are needed to determine if this outcome can be improved. Keywords: Vancomycin, Vancomycin trough concentration, Pediatric patients, Intensive care, Mortality, Factors related to mortality","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45070813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.12982/cmujns.2022.034
Fahsai Kantawong, Suraiya Sadeeyamoo, Peeraya Wongsit, M. Tungjai, P. Wanachantararak, S. Udomsom, Jianghua Yang, Ataya Sathirachinda
Abstract This study focused on observing change of gene expression after long-term treatment with high dose of crude water extract from seeds of Cuscuta japonica Choisy (Japanese dodder). The aim of this study was to study the protective and osteogenic effects of dodder seed extract at gene expression level in human gingival cells. In this research, dodder seeds were blended and boiled in water before freeze-drying to preserve as dried powder. Total phenolic content and antioxidant assay were performed. MTT assay was performed with human gingival cells. The concentrations of 100, 250 and 500 µg / mL had no effect on cell viability when cultivated for 48 hours. The extract at the concentration of 250 µg / mL was chosen to treat human gingival cells for many time points to observe the expressions of osteogenic, inflammatory, antioxidant and cancer gene markers by real-time PCR. The results showed that dodder seed water extract could increase the expressions of osteogenic markers; OPN, OCN and Col-I genes. Moreover, dodder seed water extract induced mineralization of human gingival cells cultured in 3D structure. Change of BCL2, CAS3 and LC3 expression indicated involvement in apoptotic process of dodder seed water extract. Significant changes in gene expression of antioxidant markers (GST1, SOD1 and TXNRD1), inflammatory marker (Cox-2) and epithelial cell marker (α-SMA) without change of important cancer genes were observed. These evidences suggested that seeds of Cuscuta japonica Choisy could benefit the application in regenerative medicine and alternative medicine. Keywords: Cuscuta japonica Choisy, Antioxidant, Osteogenic effects, Gene expression
{"title":"Protective and Osteogenic Effects of Crude Water Extract from Cuscuta japonica Choisy at Gene Expression Level in Human Gingival Cells","authors":"Fahsai Kantawong, Suraiya Sadeeyamoo, Peeraya Wongsit, M. Tungjai, P. Wanachantararak, S. Udomsom, Jianghua Yang, Ataya Sathirachinda","doi":"10.12982/cmujns.2022.034","DOIUrl":"https://doi.org/10.12982/cmujns.2022.034","url":null,"abstract":"Abstract This study focused on observing change of gene expression after long-term treatment with high dose of crude water extract from seeds of Cuscuta japonica Choisy (Japanese dodder). The aim of this study was to study the protective and osteogenic effects of dodder seed extract at gene expression level in human gingival cells. In this research, dodder seeds were blended and boiled in water before freeze-drying to preserve as dried powder. Total phenolic content and antioxidant assay were performed. MTT assay was performed with human gingival cells. The concentrations of 100, 250 and 500 µg / mL had no effect on cell viability when cultivated for 48 hours. The extract at the concentration of 250 µg / mL was chosen to treat human gingival cells for many time points to observe the expressions of osteogenic, inflammatory, antioxidant and cancer gene markers by real-time PCR. The results showed that dodder seed water extract could increase the expressions of osteogenic markers; OPN, OCN and Col-I genes. Moreover, dodder seed water extract induced mineralization of human gingival cells cultured in 3D structure. Change of BCL2, CAS3 and LC3 expression indicated involvement in apoptotic process of dodder seed water extract. Significant changes in gene expression of antioxidant markers (GST1, SOD1 and TXNRD1), inflammatory marker (Cox-2) and epithelial cell marker (α-SMA) without change of important cancer genes were observed. These evidences suggested that seeds of Cuscuta japonica Choisy could benefit the application in regenerative medicine and alternative medicine. Keywords: Cuscuta japonica Choisy, Antioxidant, Osteogenic effects, Gene expression","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46366434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.12982/cmujns.2022.024
G. El-Sawaf, Sara M.A.I. Adlan, D. Metwally, M. M. Abo-Ollo, Medhat Mohammed Anwar Hamed, M. S. Abdel-Latif
Abstract Staphylococcus epidermidis has gained a substantial importance in recent years because it is one of the main causative agents of nosocomial infections. It requires a predisposed host in order to switch from a normal inhabitant of the human skin to a pathogenic flora. This study aimed to use icaA/icaD genes as biomarkers in differentiating biofilm-forming S. epidermidis, obtained from patients with intravascular catheter (IVC) infections, from other saprophytic strains. Twenty isolates of S. epidermidis obtained from 100 cases of intravascular catheter infections were investigated for the presence of the intracellular adhesion icaA and icaD genes by polymerase chain reaction (PCR) and for phenotypic biofilm production by qualitative Congo red agar assay (CRA). Results: Nine (~45%) S. epidermidis isolates out of 20 isolates collected from IVC infections were positive for both CRA (produce black colonies) and icaA/icaD genes; while 11 (~55%) S. epidermidis isolates were negative for CRA and icaA/icaD genes. Detection of icaA/icaD gene is a reliable, efficient, and more rapid tool for characterizing biofilm-forming strains of S. epidermidis. Keywords: Staphylococcus epidermidis; icaA; icaD; biofilm
{"title":"Identifying biofilm-forming strains of Staphylococcus epidermidis isolated from intravascular-catheterized patients by icaA and icaD genes","authors":"G. El-Sawaf, Sara M.A.I. Adlan, D. Metwally, M. M. Abo-Ollo, Medhat Mohammed Anwar Hamed, M. S. Abdel-Latif","doi":"10.12982/cmujns.2022.024","DOIUrl":"https://doi.org/10.12982/cmujns.2022.024","url":null,"abstract":"Abstract Staphylococcus epidermidis has gained a substantial importance in recent years because it is one of the main causative agents of nosocomial infections. It requires a predisposed host in order to switch from a normal inhabitant of the human skin to a pathogenic flora. This study aimed to use icaA/icaD genes as biomarkers in differentiating biofilm-forming S. epidermidis, obtained from patients with intravascular catheter (IVC) infections, from other saprophytic strains. Twenty isolates of S. epidermidis obtained from 100 cases of intravascular catheter infections were investigated for the presence of the intracellular adhesion icaA and icaD genes by polymerase chain reaction (PCR) and for phenotypic biofilm production by qualitative Congo red agar assay (CRA). Results: Nine (~45%) S. epidermidis isolates out of 20 isolates collected from IVC infections were positive for both CRA (produce black colonies) and icaA/icaD genes; while 11 (~55%) S. epidermidis isolates were negative for CRA and icaA/icaD genes. Detection of icaA/icaD gene is a reliable, efficient, and more rapid tool for characterizing biofilm-forming strains of S. epidermidis. Keywords: Staphylococcus epidermidis; icaA; icaD; biofilm","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45530134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-21DOI: 10.12982/cmujns.2022.003
Debby Saputera, Intan Nirwana, Michael Josef Kridanto, M. Surboyo
Abstract The objective of this study was to determine the intervention mechanism of Ellagic Acid (ES) against inflammatory proteins NFKB1 and HSP70. Target Protein Analysis using SeaTarget (http://sea.bkslab.org). The compound is then analyzed for its target protein in the human body using the STITCH database (http://stitch.embl.de/). Then analyzed the interactions between proteins using the STRINGdb database (https://string-db.org/cgi/input.pl). In the analysis results with SEA, Ellagic Acid can target HSP70. Based on the results of interactions using STITCH, ellagic acid can target the Alpha Protein Kinase Catalytic Sub-unit (PRKCA). Protein Kinase Catalytic Sub-unit Alpha facilitates ellagic acid interactions with Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) and Heat Shock 60kDa Protein 1 (HSPD1). Where NFKB1 is a pleiotropic transcription factor in various processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. Ellagic acid can help reduce inflammation. It is predicted that the active compound in ellagic acid acts as an anti-inflammatory. The NFKB1 and HSP70 pathways play a role in inflammation and have activity for inflammation regulation. Keywords: Ellagic acid, HSP70, Human & Health; Inflammation, NFKB1, STITCH
{"title":"The Role of Ellagic Acid on Inflammatory Protein NFKB1 and HSP70 with STITCH Prediction","authors":"Debby Saputera, Intan Nirwana, Michael Josef Kridanto, M. Surboyo","doi":"10.12982/cmujns.2022.003","DOIUrl":"https://doi.org/10.12982/cmujns.2022.003","url":null,"abstract":"Abstract The objective of this study was to determine the intervention mechanism of Ellagic Acid (ES) against inflammatory proteins NFKB1 and HSP70. Target Protein Analysis using SeaTarget (http://sea.bkslab.org). The compound is then analyzed for its target protein in the human body using the STITCH database (http://stitch.embl.de/). Then analyzed the interactions between proteins using the STRINGdb database (https://string-db.org/cgi/input.pl). In the analysis results with SEA, Ellagic Acid can target HSP70. Based on the results of interactions using STITCH, ellagic acid can target the Alpha Protein Kinase Catalytic Sub-unit (PRKCA). Protein Kinase Catalytic Sub-unit Alpha facilitates ellagic acid interactions with Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) and Heat Shock 60kDa Protein 1 (HSPD1). Where NFKB1 is a pleiotropic transcription factor in various processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. Ellagic acid can help reduce inflammation. It is predicted that the active compound in ellagic acid acts as an anti-inflammatory. The NFKB1 and HSP70 pathways play a role in inflammation and have activity for inflammation regulation. Keywords: Ellagic acid, HSP70, Human & Health; Inflammation, NFKB1, STITCH","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41803254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-21DOI: 10.12982/cmujns.2022.012
B. Balqis, B. Lukiati, M. Amin, Siti Nur Arifah, Mochammad Fitri Atho'illah, N. Widodo
Abstract Current cancer treatment methods are still inadequate due to the complexity of the cancer progression mechanism, which involves multiple genes, proteins, and signaling pathways. The discovery and validation of novel anticancer compounds remains challenging. Garlic has many medicinal properties that can combat various diseases. Organosulfur present in garlic has been shown to induce apoptosis in cancer cells; however, the underlying mechanism of action of non-organosulfur compounds from garlic in controlling cancer cells remains unclear. The present study aimed to analyze the efficacy of organosulfur and non-organosulfur compounds, including the flavonoid, terpenoid, and saponin groups, as inhibitors of C-C chemokine receptor type 5 (CCR5) and C-X-C chemokine receptor type 4 (CXCR4), which play significant roles in the progression of cancer. To determine the interactions between the active compounds of garlic and these receptors (CCR5 and CXCR4), molecular docking was performed using the PyRx v.0.8 software. Amino acid residues were analyzed and visualized using Biovia Discovery Studio and PyMol, respectively. Non-organosulfur compounds exhibited better results than the organosulfur compounds in binding affinity analysis. Tigogenin (from the saponin group) is considered to be a CCR5 inhibitor, while lupeol (from the terpenoid group) is considered to be a CXCR4 inhibitor. In conclusion, our results suggest that garlic compounds could be promising inhibitors of CCR5 and CXCR4, which are highly expressed in cancer. However, further research is needed to validate the in vitro and in vivo activities of garlic compounds for the inhibition of cancer progression. Keywords: Anticancer agents, CCR5, CXCR4, Garlic, Organosulfur compounds, Non-Organosulfur compounds]
{"title":"Computational Study of Garlic Compounds as Potential Anti-Cancer Agents for the Inhibition of CCR5 and CXCR4","authors":"B. Balqis, B. Lukiati, M. Amin, Siti Nur Arifah, Mochammad Fitri Atho'illah, N. Widodo","doi":"10.12982/cmujns.2022.012","DOIUrl":"https://doi.org/10.12982/cmujns.2022.012","url":null,"abstract":"Abstract Current cancer treatment methods are still inadequate due to the complexity of the cancer progression mechanism, which involves multiple genes, proteins, and signaling pathways. The discovery and validation of novel anticancer compounds remains challenging. Garlic has many medicinal properties that can combat various diseases. Organosulfur present in garlic has been shown to induce apoptosis in cancer cells; however, the underlying mechanism of action of non-organosulfur compounds from garlic in controlling cancer cells remains unclear. The present study aimed to analyze the efficacy of organosulfur and non-organosulfur compounds, including the flavonoid, terpenoid, and saponin groups, as inhibitors of C-C chemokine receptor type 5 (CCR5) and C-X-C chemokine receptor type 4 (CXCR4), which play significant roles in the progression of cancer. To determine the interactions between the active compounds of garlic and these receptors (CCR5 and CXCR4), molecular docking was performed using the PyRx v.0.8 software. Amino acid residues were analyzed and visualized using Biovia Discovery Studio and PyMol, respectively. Non-organosulfur compounds exhibited better results than the organosulfur compounds in binding affinity analysis. Tigogenin (from the saponin group) is considered to be a CCR5 inhibitor, while lupeol (from the terpenoid group) is considered to be a CXCR4 inhibitor. In conclusion, our results suggest that garlic compounds could be promising inhibitors of CCR5 and CXCR4, which are highly expressed in cancer. However, further research is needed to validate the in vitro and in vivo activities of garlic compounds for the inhibition of cancer progression. Keywords: Anticancer agents, CCR5, CXCR4, Garlic, Organosulfur compounds, Non-Organosulfur compounds]","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43192009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-21DOI: 10.12982/cmujns.2022.017
S. Chotchutima, P. Boonsaen, S. Jenweerawat, Sutus Pleangkai, J. Romkaew, C. Jompuk, E. Sarobol, S. Tudsri
Abstract The objectives of the experiment were to determine plant spacing and variety to produce the suitable growth, yield components, biomass yield of field corn and nutritional value of corn silage in paddy field condition. The field experiment was conducted at the Lopburi Research Station, Khok Charoen district, Lopburi province, Thailand in 2018-2019 and was arranged in a split plot design in randomized complete block design (RCBD) with 4 replications. The main plot contained 3 plant spacings viz. 75×15, 75×20 and 75×25 cm and sub plot consisted of 5 field corn varieties (CP888, NS3, S7328, SW4452 and SW5). The results showed that wide spacing tended to produce higher ears per plant than narrow spacing. CP888 was the highest ears per plant variety. NS3 was the highest leaf/stem ratio variety. SW5 showed high leaf, stem and fresh biomass yield and it also produce ear yield closing to a high ear yield variety at 75×25 cm spacing. Moreover, SW5 also had high crude protein (CP) content, while plant spacing did not significantly affect acid detergent fiber (ADF), acid detergent lignin (ADL), ash, and lactic acid of corn silage. The widest spacing (75×25 cm) showed lower NDF than the narrowest spacing (75×15 cm). It was evident that SW5 and 75×25 cm spacing may be considered optimum for biomass yields without negatively affecting the corn silage quality. Keywords: Nutritional value, Paddy field, Plant spacing, Silage corn, Variety
摘要本试验旨在确定稻田条件下大田玉米适宜生长、产量组成、生物量产量和青贮玉米营养价值的株距和品种。田间试验于2018-2019年在泰国华富里省Khok Charoen区华富里研究站进行，采用随机完全区设计(RCBD)的分区设计，设4个重复。主样地包括75×15、75×20和75×25 cm 3个株距，子样地包括5个大田玉米品种(CP888、NS3、S7328、SW4452和SW5)。结果表明，宽间距的单株穗数高于窄间距。CP888是单株穗数最高的品种。NS3是叶片/茎比最高的品种。SW5的叶、茎、鲜生物量产量均较高，穗产量在75×25 cm间距上也接近高产品种。SW5粗蛋白质(CP)含量较高，株距对玉米青贮酸性洗涤纤维(ADF)、酸性洗涤木质素(ADL)、灰分和乳酸的影响不显著。最宽间距(75×25 cm)的NDF低于最窄间距(75×15 cm)。可见，SW5和75×25 cm间距可被认为是生物量产量的最佳选择，而不会对玉米青贮品质产生负面影响。关键词:营养价值，稻田，株距，青贮玉米，品种
{"title":"Influence of Varieties and Spacings on Growth, Biomass Yield and Nutritional Value of Corn Silage in Paddy Field","authors":"S. Chotchutima, P. Boonsaen, S. Jenweerawat, Sutus Pleangkai, J. Romkaew, C. Jompuk, E. Sarobol, S. Tudsri","doi":"10.12982/cmujns.2022.017","DOIUrl":"https://doi.org/10.12982/cmujns.2022.017","url":null,"abstract":"Abstract The objectives of the experiment were to determine plant spacing and variety to produce the suitable growth, yield components, biomass yield of field corn and nutritional value of corn silage in paddy field condition. The field experiment was conducted at the Lopburi Research Station, Khok Charoen district, Lopburi province, Thailand in 2018-2019 and was arranged in a split plot design in randomized complete block design (RCBD) with 4 replications. The main plot contained 3 plant spacings viz. 75×15, 75×20 and 75×25 cm and sub plot consisted of 5 field corn varieties (CP888, NS3, S7328, SW4452 and SW5). The results showed that wide spacing tended to produce higher ears per plant than narrow spacing. CP888 was the highest ears per plant variety. NS3 was the highest leaf/stem ratio variety. SW5 showed high leaf, stem and fresh biomass yield and it also produce ear yield closing to a high ear yield variety at 75×25 cm spacing. Moreover, SW5 also had high crude protein (CP) content, while plant spacing did not significantly affect acid detergent fiber (ADF), acid detergent lignin (ADL), ash, and lactic acid of corn silage. The widest spacing (75×25 cm) showed lower NDF than the narrowest spacing (75×15 cm). It was evident that SW5 and 75×25 cm spacing may be considered optimum for biomass yields without negatively affecting the corn silage quality. Keywords: Nutritional value, Paddy field, Plant spacing, Silage corn, Variety","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43963723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-21DOI: 10.12982/cmujns.2022.009
Muhammad Zainuddin, D. Pringgenies, O. Radjasa, H. -
Abstract Sponges are marine organisms that have associations with microorganisms. The association fungi in sponges have a chemical and ecological relationship. These bioactive compounds have potential in the pharmaceutical field. This research aimed to explore bioactive compounds of association fungi in sponges that live in coral, seaweed and mangrove ecosystems. The research consisted of isolation, purification, extraction, antibacterial testing, molecular identification, antioxidant testing and toxicity testing. The research was conducted on March to September 2020 in Karimunjawa Island. The results showed that the sponge association fungi isolates had antibacterial activity against the Multi Drug Resistant (MDR) pathogens Staphylococcus aureus (Sa), Escherichia coli (Ec), and Staphylococcus epidermidis (Se), Pseudomonas aeruginosa (Pa) and the fungi Candida albicans (Ca). The identification results of the active association fungi were Circinelloides sp., Xylariaceae sp., Trichoderma asperellum, Aspergillus sp. and Pleosporales sp. . The extracts of these fungi had antioxidant activity with an IC50 value of 1071,62; 643,35; 1020,32; 805,70 and 784,31 mg/L. Furthermore, they also had an LC50 value of 462,67; 355,47; 504,92; 482,15 and 435,88 mg/L. Keywords: Antimicrobial, Antioxidant, Fungi, Sponge, Symbiosis
{"title":"Natural Bioactive Compounds of Sponge-Associated Fungi with Three Marine Ecosystems in Karimunjawa Island, Indonesia","authors":"Muhammad Zainuddin, D. Pringgenies, O. Radjasa, H. -","doi":"10.12982/cmujns.2022.009","DOIUrl":"https://doi.org/10.12982/cmujns.2022.009","url":null,"abstract":"Abstract Sponges are marine organisms that have associations with microorganisms. The association fungi in sponges have a chemical and ecological relationship. These bioactive compounds have potential in the pharmaceutical field. This research aimed to explore bioactive compounds of association fungi in sponges that live in coral, seaweed and mangrove ecosystems. The research consisted of isolation, purification, extraction, antibacterial testing, molecular identification, antioxidant testing and toxicity testing. The research was conducted on March to September 2020 in Karimunjawa Island. The results showed that the sponge association fungi isolates had antibacterial activity against the Multi Drug Resistant (MDR) pathogens Staphylococcus aureus (Sa), Escherichia coli (Ec), and Staphylococcus epidermidis (Se), Pseudomonas aeruginosa (Pa) and the fungi Candida albicans (Ca). The identification results of the active association fungi were Circinelloides sp., Xylariaceae sp., Trichoderma asperellum, Aspergillus sp. and Pleosporales sp. . The extracts of these fungi had antioxidant activity with an IC50 value of 1071,62; 643,35; 1020,32; 805,70 and 784,31 mg/L. Furthermore, they also had an LC50 value of 462,67; 355,47; 504,92; 482,15 and 435,88 mg/L. Keywords: Antimicrobial, Antioxidant, Fungi, Sponge, Symbiosis","PeriodicalId":10049,"journal":{"name":"Chiang Mai University journal of natural sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45834357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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