Environmental DNA technology develops rapidly in the field of biodiversity detection. Selecting appropriate primers may be one of the key questions. However, there is currently a lack of systematic studies on differences in primer detection efficiencies. This research investigates the efficiency of six universal primers (12S, 16S, 18S, MiFish, Cytb, and COI) in detecting fish species across diverse aquatic ecosystems using an environmental DNA approach. The research spans five study areas, representing marine, river, wetland, lake, and reservoir ecosystems. Illumina MiSeq sequencing and bioinformatics tools were employed for primer performance evaluation. Results indicate that MiFish consistently outperforms other primers, detecting the highest number of fish species across all ecosystems and exhibiting superior taxonomic coverage. Furthermore, marine ecosystems consistently show higher detection numbers across all primers. The absence of commonly identified species detected by all primers emphasizes the necessity of using multiple primers for a comprehensive assessment. This study provides valuable insights into the strengths and limitations of universal primers, highlighting the importance of primer selection for accurate eDNA-based fish monitoring. The findings contribute to the scientific basis for the comprehensive management of aquatic ecosystems, assisting researchers and ecosystem managers in screening suitable fish universal primers for eDNA methods. The study also calls for further research into factors influencing primer performance and encourages the refinement of primers to enhance biodiversity monitoring precision in various ecosystems.
Microplastics (MPs) are recognized as emerging contaminants due to their small size, hazardous nature, and widespread abundance in the environmental compartments. As the largest sink of MPs, oceans are currently the main focus. However, the question about MP particles applies to all environmental niches, including natural wetlands. Unfortunately, natural wetlands have been not explored much in comparison to the ocean and the terrestrial ecosystem for MP occurrence and its toxicity. Meanwhile, these natural wetlands offer important ecological services and have a high biodiversity, both of which could be compromised by the rising levels of MP contamination in such systems. However, due to limited research in natural wetlands possible ecological repercussions are still lacking. Even there are uncertainties about MPs in the wetlands regarding their distribution and fate. Since these wetlands have the potential to trap plastics and could act as a sink for MPs, it becomes essential to investigate the natural wetland for MP contamination and its possible toxicological risks in wetlands. This review summarised the source, potential sink and toxicological consequences of MPs in natural wetlands. The review also illustrated the advancements in the technologies aiming to remove plastic debris from the natural environment.
In recent years, Fenhe River Basin (FRB) wetlands have been facing severe challenges due to climate change and human activities. It was of great significance to understand the dynamic changes of FRB wetlands and their driving factors for ecological protection. Based on the land use data, runoff, rainfall and GDP data of FRB from 1980 to 2020, the evolution characteristics and driving factors of wetland pattern in FRB were analyzed. The results showed that the wetland types in FRB mainly included paddy, reservoir, river, beach and marsh. Among them, natural wetlands accounted for 72.46 %, and constructed wetlands accounted for 27.53 %. The main types of wetlands in FRB were river, reservoirs and beach, which account for 92.26 % of the whole wetland area. However, the dominant position of reservoir patch was more obvious than the others during 1980–2020. Landscape index showed that the shape of landscape tended to be regular and became more fragmentation. The transfer between wetlands and other land use types mainly occurred in the northern and southern parts of the FRB, and the main conversion types were occurred between dry land and wetlands, grassland and wetlands. Both natural and human factors drove the evolution pattern of wetlands, but the emphases were different. Runoff had a significant effect on wetland pattern at landscape level, while rainfall had a more significant effect on wetland evolution at class level. Urbanization rate and GDP had important effects on the evolution of wetland pattern at both class and landscape levels.
The Tutua-Bura-Angoben Shelter Belt project in Ghana, aimed at combating desertification and land degradation, faces an unexpected threat from heavy metal contamination. This study investigates the levels and spatial distribution of arsenic (As), lead (Pb), copper (Cu), and zinc (Zn) within the forest reserve, focusing on the implications for environmental health and sustainable development goals (SDGs). Using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), 195 soil samples from the forest reserve were analyzed for heavy metal concentrations. The results reveal significant contamination, with arsenic levels ranging from 3.19 to 138.63 ppm, and an alarming 26 % of the reserve exceeding the 20 ppm threshold for arsenic. Copper, lead, and zinc showed lower contamination levels, with mean concentrations of 13.83 ppm, 7.63 ppm, and 27.53 ppm, respectively. Spatial interpolation using kriging in ArcGIS highlighted localized hotspots of arsenic contamination, primarily influenced by nearby Artisanal Small-Scale Gold Mining (ASGM) activities. The study underscores the urgent need for targeted environmental management strategies, including stricter waste management protocols, sustainable mining practices, and community engagement to mitigate the contamination risks and preserve the ecological integrity of the Tutua-Bura-Angoben Shelter Belt.
Aquatic habitats serve as pollutant reservoirs and experience massive pollution disasters due to anthropogenic pressure. The effect of the Water-Soluble Fraction (WSF) of burnt tire ash (BTA) was investigated on Clarias gariepinus juvenile using blood biomarkers as well as liver and gill histology. The model fish were exposed to 5 % (0.56 g/L), 10 % (1.12 g/L) and 20 % (2.24 g/L) of 96-hr LC50 (11.2 g/L) value of BTA sub-chronic doses during the experiment. Hematological indices, the liver, and gill histology were studied throughout the 28-day exposure study, which was followed by a 14-day post-exposure trial. The studies revealed that BTA-exposed fish had reduced red blood cells, packed cell volume, and hemoglobin levels with time, whereas white blood cells and leukocyte numbers increased. Concentration-dependent histopathologic abnormalities such as hyperplasia, telangiectasis, hemorrhage, desquamation, lamellar edema, and vacuolation were observed in the gill of the affected fish, in contrast to the control. Likewise, the presence of histopathologic abnormalities in the liver of BTA-exposed fish, such as vacuolar degeneration, hyperplasia, dilated portal vein, pyknotic nucleus formations, hypertrophy hepatocytes, and aggregated macrophages, was concentration-dependent. Following apparent histologic deformities that persisted in the tissues after recovery, healing from BTA exposure was only partial. C. gariepinus is an essential bioindicator of burnt tire ash’s ecotoxicological impact. Point and non-point traces of burnt-tire ash to the aquatic systems may harm aquatic animal species, thus necessitating preventative actions.
Potentially toxic elements (PTEs) are naturally available in the environment; however, anthropogenic activities encourage their high concentration, posing environmental and human health risks. The ecotoxicological status, source apportionment and health risk assessment of PTEs (Iron (Fe), Nickel (Ni), Cadmium (Cd), Selenium (Se), Zinc (Zn), Lead (Pb), Chromium (Cr) and Copper (Cu)) in creeks (Fibiri and Iwoama) along Bonny River were examined through laboratory analysis (American Public Health Association standard), indicators and statistical techniques. The PTEs concentration of surface water and sediment for both creeks exceeded the WHO allowable limit, and the surface water trended as Cr > Pb > Fe > Ni > Cd > Zn > Cu > Se for Fibiri creeks, Fe > Cr > Pb > Ni > Cd > Zn > Cu > Se for Iwoama creek while the sediment trended as Fe > Pb > Ni > Cr > Zn > Cd > Cu > Se for both creeks. The contamination factor (CF) of the creek’s sediment showed low CF (Zn and Cu), moderate CF (Ni, Pb and Cr) and very high CF (Fe and Cd), while risk index status ranged from low risk (Ni, Zn, Pb, Cr and Cu), moderate risk (Fe) and significantly high risk (Cd). The human health risk revealed that children could be exposed to potential non-carcinogenic effect (HI > 1) with Zn (1.64 and 2.03), and the Total Carcinogenic Risk (TCR) for children indicated high carcinogenic risk (TCR > 1 × 10−4) for Ni, Pb, Cr and Cd of the creeks’ environmental mediums. The similarity in the trend and status of the creeks suggested that both environments are exposed to similar anthropogenic activities impact.