Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie最新文献

The formation and persistence of latexagglutination-inhibiting (LAH), neutralizing (N), and complement-fixing (CF) antibodies were in Rhesus monkeys after experimental infection with coxsackievirus type B4.

In all 3 animals the LAH antibodies showed a significant decrease between the 49th and 56th day after infection. In 2 cases the CF antibodies declined to 25% of their maximum titer on day 84 after infection, and in 2 animals the N antibodies declined between the 70th and 84th day after infection.

The 2 related mouse strains C3H/HeJ and C3H/Han differ in their response to endotoxin. C3H/HeJ mice are endotoxin-resistant, C3H/Han mice are - like all other mice - endotoxin-sensitive. The uptake of fluorescent intraperitoneally injected lipopolysaccharide was similar in both mouse strains. The intraperitoneal injection of lipopolysaccharide produced a higher mobilization of neutrophil granulocytes in resistant C3H/HeJ mice than in C3H/Han mice. An opposite pattern of mobilization of neutrophil granulocytes could be observed after injection of the prostaglandins E 1, E 2, and F 1 α; when both mouse strains were compared, a normal mobilization was observed in C3H/Han mice C3H/HeJ mice showed a markedly decreased mobilization.

If the difference between the 2 strains resides in a single gene locus it is to be assumed that lipopolysaccharide resistance is controlled by the same gene which also effects an impaired response to neutrophil mobilization after prostaglandin application.

The peripheral blood lymphocyte subpopulations of 18 patients with rheumatoid arthritis were compared with those of 10 healthy donors using minor modifications of the mixed immunofluorescence and rosette test (1), rosette test using human 0 RhD erythrocytes coated with anti-RhD antibodies (2) and the mixed rosette test (3).

It was found that the percentages of lymphocytes exhibiting both immunoglobulin receptors and receptors for sheep erythrocytes, and lymphocytes with receptors for Fc part of IgG in the rheumatoid patients were increased. A hypothesis for the origin of these subpopulations is discussed.

The authors report on the anti-tetanus reimmunization of 76 diabetic children and adolescents hospitalized in the Diabetic Ward for Children in Varshetz. All patients had insulin-deficient diabetes and they were treated with insulin Lente. The diet was limited and physiological. Thirty-nine of the patients had diabetes in a state of compensation, while the remaining 37 patients were in a state of decompensation without acetonuria.

The reimmunization was well tolerated and no strong general and local reactions were observed.

The authors do not establish statistically significant differences during an overnight period in the blood sugar level and the degree of glucosuria before and after reimmunization. They conclude that the immunization does not disturb the metabolic processes.

On the 20th day after the reimmunization 98.6% of the children showed a high antitoxic titre, which suggests that the capacity of diabetics to elaborate a good anti-tetanus immunity is unimpaired.

It is recommended that patients with diabetes should be immunized against tetanus during a stage of stabilized diabetes metabolism.

The mechanism of L-agglutination (LA) was studied by fractionating human sera by means of Sephadex G 200 gel filtration. LA was found to be mediated by 2 factors: the rheumatoid factor (RF) as a macroglobulin, occuring only in sera from patients with rheumatoid arthritis, and an IgG globulin, found also in normal sera, probably a higly prevalent incomplete streptococcal antibody. RF-containing IgM fractions, by themselves devoid of LA activity, can be rendered positive not only by IgG from the same serum, but also by IgG from normal sera or by commercial human IgG. After absorption of sera containing the RF with streptococci LA disappears; by addition of normal serum, IgG from normal serum or human IgG LA titers were attained similar to those of the respective native serum.

A treatment of RF-containing sera with mercaptoethanol leads to a largely parallel disappearance of LA and latex fixation. Three substrains of Streptococcus pyogenes SF 130 (type 1), varying in their endowment with type-specific antigens, reacted similarly in LA.