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Effect of spray aeration on unregulated drinking water disinfection byproducts 喷雾曝气对非调节饮用水消毒副产物的影响
Pub Date : 2023-05-22 DOI: 10.1002/aws2.1341
Kathleen R. Beaudoin, Jeanne M. VanBriesen, David A. Dzombak

Spray aeration is a comparatively low-cost option to reduce trihalomethane (THM) concentrations in distribution system storage. The effect of spray aeration on unregulated, toxic disinfection by-products was investigated in a bench-scale apparatus in closed-tank and open-tank experiments. In the closed-tank experiments, initially over 90% of THMs, trichloroacetonitrile, and chloropicrin were removed, while 36% of dihaloacetonitriles were removed. However, due to the buildup of concentrations in the tank headspace, removal rates for all compounds decreased significantly with time, falling to 44% removal of THMs, 76% removal of trichloroacetonitrile, and 58% removal of chloropicrin. Removal of dihaloacetonitriles decreased to zero. In the open-tank experiments, THM removal rates were high and did not decrease with time. Considering the results with open and closed tanks as approximate bounds for performance expected under real-world conditions in storage tanks, the findings suggest that spray aeration may not significantly reduce haloacetonitrile-related toxicity while halonitromethane-related toxicity may be reduced.

喷雾曝气是降低分配系统储存中三卤甲烷(THM)浓度的一种相对低成本的选择。在封闭池和开放池实验中,在实验室规模的装置中研究了喷雾曝气对未调节的有毒消毒副产物的影响。在密闭罐实验中,最初去除了90%以上的THM、三氯乙腈和氯化苦,同时去除了36%的二卤代乙腈。然而,由于罐顶部空间中浓度的增加,所有化合物的去除率都随着时间的推移而显著下降,THMs的去除率降至44%,三氯乙腈的去除率为76%,氯化苦的去除率则降至58%。二卤代乙腈的去除率降至零。在开罐实验中,THM的去除率很高,并且不会随着时间的推移而降低。考虑到开放式和封闭式储罐的结果是储罐在真实世界条件下预期性能的近似界限,研究结果表明,喷雾曝气可能不会显著降低卤代乙腈相关的毒性,而卤代硝基甲烷相关的毒性可能会降低。
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引用次数: 0
Interlaboratory comparisons of cyanotoxin analysis by ELISA and LC–MS/MS 用ELISA和LC–MS/MS进行蓝藻毒素分析的实验室间比较
Pub Date : 2023-05-19 DOI: 10.1002/aws2.1342
Matthew D. Prescott, Ai Jia, Yingbo C. Guo, George D. Di Giovanni, Wei L. Li, Eduardo A. Garcia, Brett J. Vanderford, Andrew D. Eaton

Two commonly used methods for cyanotoxin analysis are enzyme-linked immunosorbent assay (ELISA) and liquid chromatography/tandem mass spectrometry (LC–MS/MS). Two rounds of interlaboratory comparisons of ELISA and LC–MS/MS analyses were conducted with 12 participating laboratories to evaluate method performances in various matrices, including cyanobacterial bloom and drinking water samples. Fifteen cyanotoxins, including 12 microcystin variants, nodularin, anatoxin-a, and cylindrospermopsin were evaluated. The impact of sample matrices, preservatives, and quenching reagents was assessed, and no substantial effects were observed. Overall, comparable results were obtained among laboratories performing ELISA and LC–MS/MS analyses, respectively. ELISA results for fortified samples matched more closely with those from LC–MS/MS when microcystin cross-reactivities were considered, providing data 26% closer to theoretical values on average. This study demonstrates that understanding the effect of cross-reactivities when comparing ELISA and LC–MS/MS results and considering potential variabilities in commercial standards is important when interpreting data from these two methods.

两种常用的蓝藻毒素分析方法是酶联免疫吸附测定法(ELISA)和液相色谱/串联质谱法(LC–MS/MS)。与12个参与实验室进行了两轮ELISA和LC–MS/MS分析的实验室间比较,以评估方法在各种基质中的性能,包括蓝藻水华和饮用水样品。评估了15种蓝藻毒素,包括12种微囊藻毒素变体、结节藻毒素、类毒素-a和柱状藻毒素。评估了样品基质、防腐剂和淬火试剂的影响,未观察到实质性影响。总体而言,分别在进行ELISA和LC–MS/MS分析的实验室中获得了可比较的结果。当考虑微囊藻毒素的交叉反应时,强化样品的ELISA结果与LC–MS/MS的结果更接近,提供的数据平均接近理论值26%。这项研究表明,在解释这两种方法的数据时,在比较ELISA和LC–MS/MS结果时,理解交叉反应的影响并考虑商业标准中的潜在变异性是很重要的。
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引用次数: 0
Impact of nitrogen and phosphorus on the growth and microcystin-related gene expression of Microcystis aeruginosa PCC 7806 氮磷对铜绿微囊藻PCC 7806生长及微囊藻毒素相关基因表达的影响
Pub Date : 2023-05-19 DOI: 10.1002/aws2.1343
Arash Jafarzadeh, Duc Phan, Vikram Kapoor

Harmful algal blooms (HABs) have incurred numerous health problems through drinking water or exposure. A better understanding of the growth kinetics and toxin production under different environmental conditions will help develop tools for early-warning indicators for predicting HABs. Lab-scale experiments were performed with Microcystis aeruginosa PCC 7806 under varying N:P ratios and temperatures. We observed different growth behaviors for cultures growing at the three temperatures (20, 25, and 30°C). M. aeruginosa exhibited higher growth rates at higher N:P ratios during the incubation at 25 and 30°C. Microcystis 16S rRNA gene abundance correlated well with the cell growth parameters. Based on RT-qPCR data, the transcripts of mcyA, mcyD, and mcyE were occasionally detected with N:P of 76.86 showing highest detections of all gene transcripts. Intracellular microcystin (MC) production corresponded well with the presence of gene transcripts, while no extracellular MC was detected. These findings would provide valuable insights and beneficial guidance to further explore and predict the impacts of cyanobacterial blooms in freshwater systems.

有害藻华(HABs)由于饮用水或暴露在环境中而引发了许多健康问题。更好地了解不同环境条件下的生长动力学和毒素产生,将有助于开发预测赤潮的预警指标工具。用铜绿微囊藻PCC 7806在不同的氮磷比和温度下进行实验室规模的实验。我们观察到在三种温度(20、25和30°C)下生长的培养物的不同生长行为。在25和30°C的培养过程中,铜绿假单胞菌在较高的氮磷比下表现出较高的生长速率。微囊藻16S rRNA基因丰度与细胞生长参数呈正相关。基于RT-qPCR数据,偶尔检测到mcyA、mcyD和mcyE的转录物,N:P为76.86,显示出所有基因转录物中最高的检测率。细胞内微囊藻毒素(MC)的产生与基因转录物的存在非常一致,而没有检测到细胞外微囊藻毒素。这些发现将为进一步探索和预测淡水系统中蓝藻水华的影响提供有价值的见解和有益的指导。
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引用次数: 0
Microcystins at the tap: A closer look at unregulated drinking water contaminants 水龙头上的微囊藻毒素:仔细观察不受监管的饮用水污染物
Pub Date : 2023-05-12 DOI: 10.1002/aws2.1337
Beckye Stanton, Amy Little, Lisa Miller, Gina Solomon, Sarah Ryan, Susan Paulukonis, Stefan Cajina

This case study compared microcystins in tap water from 18 public water systems (PWSs) and 46 households with private intakes or nearshore wells (called self-supplied water systems [SSWS]) from Clear Lake, California during a major cyanobacterial bloom in 2021. Microcystin concentrations were below the US EPA health advisory (HA; 0.3 μg/L) for PWS with Clear Lake intakes and for SSWS drawing from wells. However, for SSWS with Clear Lake intakes (intake SSWS), 24 of 79 microcystin samples collected were at or above the HA (maximum 3.9 μg/L). Further, microscopy showed that 20 of 28 tap samples (post-treatment) from intake SSWS contained either cyanobacteria or algal cells. This demonstrates that robust multi-barrier treatment systems at PWS reliably removed microcystins, while the less sophisticated treatment systems at intake SSWS were compromised during a major cyanobacterial bloom. Intake SSWS should consider alternative sources of drinking water, including physically connecting to an adjacent PWS or drilling a well.

本案例研究比较了2021年加州克利尔湖18个公共供水系统(PWS)和46个拥有私人取水口或近岸水井(称为自备供水系统[SSWS])的家庭的自来水中的微囊藻毒素。微囊藻毒素浓度低于美国环保局健康建议(HA;0.3 μg/L),用于带有Clear Lake进水口的PWS和从井中抽取的SSWS。然而,对于有Clear Lake摄入量的SSWS(摄入量SSWS),在收集的79个微囊藻毒素样本中,有24个达到或超过HA(最大3.9 μg/L)。此外,显微镜检查显示,来自摄入SSWS的28个水龙头样品(后处理)中有20个含有蓝藻或藻类细胞。这表明,PWS的强大的多屏障处理系统可靠地去除了微囊藻毒素,而SSWS的不太复杂的处理系统在一次严重的蓝藻爆发期间受到了损害。取水SSWS应考虑替代饮用水源,包括物理连接到相邻PWS或钻井。
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引用次数: 0
Treatability of 18 taste and odor compounds in drinking water using oxidation 氧化法处理饮用水中18种味觉和气味化合物
Pub Date : 2023-05-03 DOI: 10.1002/aws2.1340
Susheera Pochiraju, Craig Adams, Christiane Hoppe-Jones, Samar Maalouf, Lauren Weinrich

The occurrence of taste and odor (T&O) compounds in drinking water can lead to public concern about quality when left unaddressed, therefore suitable treatment is needed. This study evaluated the treatability of 18 T&O compounds using chlorine, permanganate, and ozone in MilliQ and river water. Nine T&O compounds showed complete oxidation (>99% removal) in all water matrices by ozone. Most of the remaining T&O compounds were removed to >50% by ozone. Alkalinity, pH, and natural organic matter (NOM) had a significant impact on the decay of ozone. Chlorine and permanganate were ineffective (removal <30%) for 2-methylisoborneol (2-MIB), geosmin, hexanal, pyrazines, and haloanisoles. Although chlorine achieved complete removal for alkyl sulfides and indole, it was only partially effective for aldehydes and ketones. Permanganate was more effective than chlorine in oxidizing unsaturated aldehydes and ketones. Increased NOM and pH reduced oxidation effectiveness for chlorine, but not permanganate.

饮用水中味道和气味(T&;O)化合物的出现,如果不加以处理,会导致公众对水质的担忧,因此需要适当的处理。这项研究评估了18 T&;O在MilliQ和河水中使用氯、高锰酸盐和臭氧的化合物。九个T&;O化合物在所有的水基质中显示出被臭氧完全氧化(>99%去除)。剩下的大部分T&;O化合物被去除至>;50%的臭氧。碱度、pH值和天然有机物(NOM)对臭氧的衰变有显著影响。氯和高锰酸盐对2-甲基异戊醇(2-MIB)、geosmin、己醛、吡嗪和卤代苯甲醚无效(去除率<30%)。尽管氯对烷基硫化物和吲哚实现了完全去除,但对醛和酮仅部分有效。在氧化不饱和醛和酮方面,高锰酸钾比氯更有效。NOM和pH的增加降低了氯的氧化效率,但没有降低高锰酸盐的氧化效率。
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引用次数: 0
A literature-engaged Delphi approach for water quality management in building water systems 建筑供水系统水质管理的德尔菲方法
Pub Date : 2023-05-03 DOI: 10.1002/aws2.1339
Md Rasheduzzaman, Rajveer Singh, Charles N. Haas, Mira S. Olson, Patrick L. Gurian

We used the Delphi method to identify areas of consensus and disagreement in managing building water systems. One hundred and six experts were invited, 25 of whom completed both rounds. More than 95% of experts agreed that current plumbing codes are not adequate and return loop temperature should be monitored. A notably low percentage of experts agreed on the required level of chloramine (i.e., 32%) and flushing frequency (22%), despite these being common control strategies. Consensus was not achieved for temperature and disinfectant residuals, but a plurality of respondents indicated that a temperature of 55°C was appropriate and that low but detectable levels of disinfectant residuals are appropriate. After Round 2, a majority of the experts agreed that the knowledge syntheses provided were helpful and unbiased. Findings from this study will help stakeholders by providing helpful insights on plumbing code, design factors, operational factors, and water quality monitoring practices.

我们使用德尔菲方法来确定在管理建筑供水系统方面存在共识和分歧的领域。共邀请了106名专家,其中25人完成了两轮培训。超过95%的专家一致认为,目前的管道规范不够,应监测回路温度。尽管氯胺的要求水平(即32%)和冲洗频率(22%)是常见的控制策略,但达成一致的专家比例明显较低。在温度和消毒剂残留方面没有达成共识,但许多受访者表示,55°C的温度是合适的,低但可检测的消毒剂残留水平是合适的。在第二轮会议之后,大多数专家一致认为,所提供的综合知识是有益和公正的。这项研究的结果将通过提供对管道规范、设计因素、操作因素和水质监测实践的有益见解来帮助利益相关者。
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引用次数: 0
Evaluation of sequential dosing of potassium permanganate for microcystin-LR removal 高锰酸钾序贯给药去除微囊藻毒素LR的评价
Pub Date : 2023-04-26 DOI: 10.1002/aws2.1338
Maycee Hurd, Allison A. MacKay, Juliana R. Laszakovits

Permanganate can be scavenged by dissolved organic matter (DOM), which decreases microcystin (MC) removal efficiency, and permanganate attack on cyanobacterial cells can damage cells, resulting in the release of additional MCs. Here, the impact of sequential permanganate doses on MC-LR removal in the presence of two DOM isolates (terrestrial- and algal-derived) was examined. Although sequential permanganate dosing reduced the competition for permanganate from each DOM isolate, the overall MC removal efficiency was not as high when multiple smaller doses of permanganate were applied compared to one single dose. Potassium permanganate was not observed to lyse Microcystis cells up to 10 mg L−1. Sequential permanganate dosing did not alter the extent of lysis observed for a model bacterium. Mathematical simulations of permanganate oxidation in the presence of DOM suggested that sequential dosing would be most beneficial for enhancing MC-LR removal when a small portion of highly reactive DOM exists.

溶解有机物(DOM)可以清除高锰酸盐,从而降低微囊藻毒素(MC)的去除效率,高锰酸盐对蓝藻细胞的攻击会损伤细胞,导致额外的MC释放。在此,研究了在存在两种DOM分离物(陆生和藻类衍生)的情况下,连续高锰酸盐剂量对MC-LR去除的影响。尽管连续高锰酸盐给药减少了每个DOM分离物对高锰酸盐的竞争,但与单次给药相比,当使用多个较小剂量的高锰酸盐时,总的MC去除效率没有那么高。高达10的高锰酸钾不能裂解微囊藻细胞 毫克 L−1。连续给药高锰酸盐不会改变模型细菌的裂解程度。DOM存在下高锰酸盐氧化的数学模拟表明,当存在一小部分高反应性DOM时,顺序给药对提高MC-LR的去除最为有利。
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引用次数: 0
Management of point-of-use and point-of-entry for regulatory compliance: Survey of state administrators 管理法规遵从性的使用点和进入点:对州行政人员的调查
Pub Date : 2023-04-26 DOI: 10.1002/aws2.1335
Katherine Alfredo, Madelyn Wilson, Alan Roberson
The USEPA (United States Environmental Protection Agency) Lead and Copper Rule Revisions allow the use of distributed treatment approaches such as point‐of‐use (POU) and point‐of‐entry (POE) treatment for systems with 10,000 connections or less as a compliance strategy. However, this poses an opportunity for the USEPA to reevaluate system size recommendations for distributed treatment. The current research uses online surveys and semi‐structured interviews (SSIs) to highlight the general sentiment of state regulators managing POU/POE devices and inquiries. Analysis of the 43 survey responses and 13 SSIs revealed that most state regulators described systems of approximately 30–50 connections as the most successful. Resident cooperation, operation and maintenance, monitoring, and the actual implementation of distributed treatment approaches were repeatedly listed as the greatest concerns. As the use of distributed treatment continues to expand, the water sector must devote research efforts to quantitatively determining the drivers of success as well as highlighting clear indicators of potential failure.
USEPA(美国环境保护局)铅和铜规则修订允许使用分布式处理方法,如使用点(POU)和入口点(POE)处理,将10000个或更少连接的系统作为合规策略。然而,这为美国环保局重新评估分布式治疗的系统规模建议提供了机会。目前的研究使用在线调查和半结构化访谈(SSI)来突出管理POU/POE设备和查询的州监管机构的普遍情绪。对43份调查回复和13份SSI的分析显示,大多数州监管机构将大约30-50个连接的系统描述为最成功的系统。居民合作、运营和维护、监测以及分布式治疗方法的实际实施一再被列为最大的关切。随着分布式处理的使用不断扩大,水务部门必须投入研究工作,从数量上确定成功的驱动因素,并突出潜在失败的明确指标。
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引用次数: 1
Multi-objective optimization models for the renewal planning of multiple asset classes 多资产类别更新规划的多目标优化模型
Pub Date : 2023-04-25 DOI: 10.1002/aws2.1336
Thomas Ying-Jeh Chen, Eric Wang, Nicole Pasch, Amin Ganjidoost

Managing the aging infrastructure of water distribution systems presents a challenge for many utilities. With various asset types competing for limited dollars, designing an effective asset management program is a resource allocation problem. Mobilization of equipment and crew is a significant cost (typically 2%–10%) within any capital improvement program. Therefore, selecting projects that target multiple asset classes together can reduce mobilization and help utilities stretch their budgets further. This research presents a process for modeling the joint renewal planning of multiple asset classes. The problem is framed as a dual-objective optimization, where the selection of project areas aims to maximize lead service line removal and water meter changeout together. A case study from a Midwest utility is presented, and empirical data suggests the dual-objective approach effectively reduces duplicate interventions in the same regions. Equity considerations are also examined, where constraints are added to enforce system-wide project selection. Results show that the sensitivity of the objective toward equity is dependent on the underlying spatial distribution of the target asset itself, where uneven spread of the target asset leads to greater negative impact on model performance.

管理老化的配水系统基础设施对许多公用事业公司来说是一项挑战。在各种资产类型争夺有限资金的情况下,设计一个有效的资产管理计划是一个资源分配问题。在任何资本改善计划中,设备和人员的调动都是一项巨大的成本(通常为2%-10%)。因此,选择同时针对多个资产类别的项目可以减少动员,并帮助公用事业公司进一步扩大预算。本研究提出了一个对多个资产类别的联合更新计划进行建模的过程。该问题被定义为一个双目标优化问题,其中项目区域的选择旨在最大限度地减少引线服务线和更换水表。一个中西部公用事业公司的案例研究表明,双目标方法有效地减少了同一地区的重复干预。还审查了公平考虑因素,其中增加了限制因素,以强制全系统项目选择。结果表明,目标对权益的敏感性取决于目标资产本身的潜在空间分布,其中目标资产的不均匀分布会对模型性能产生更大的负面影响。
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引用次数: 0
The importance of uranium isotope composition for gross alpha activity regulation 铀同位素组成对总α活性调节的重要性
Pub Date : 2023-04-19 DOI: 10.1002/aws2.1329
Sean R. Scott, Amy K. Wiersma, Gary Krinke

Uranium is a regulated contaminant (maximum contaminant level [MCL] 30 μg/L) that contributes to the gross alpha activity of groundwater. The adjusted gross alpha activity (MCL 15 pCi/L) is determined by subtracting the total uranium activity from the measured gross alpha activity. U.S. water utilities can use mass-based and radiochemical analysis methods for compliance monitoring of uranium. Mass-based measurements use a conversion factor of 0.67 pCi/μg of uranium to calculate the adjusted gross alpha activity. This conversion factor assumes that the activity of 234U equals 238U. Here, we present two decades of uranium isotope data measured by alpha spectrometry that shows 234U activity typically exceeds 238U. Using mass-based measurements, the total uranium activity is biased low causing artificial exceedances of the adjusted gross alpha activity. Therefore, water utilities with gross alpha activities >15 pCi/L should utilize radiochemical analyses for uranium for the most accurate calculation of adjusted gross alpha activity.

铀是一种受管制的污染物(最大污染物水平[MCL]30 μg/L),这有助于地下水的总α活性。调整后的总α活性(MCL 15 pCi/L)通过从测量的总α活性中减去总铀活性来确定。美国自来水公司可以使用基于质量的放射化学分析方法对铀进行合规性监测。基于质量的测量使用0.67的转换系数 pCi/μg铀,以计算调整后的总α活性。这个转换因子假定234U的活度等于238U。在这里,我们展示了20年来通过阿尔法光谱法测量的铀同位素数据,显示234U的活性通常超过238U。使用基于质量的测量,总铀活性被偏置为较低,导致人工超过调整后的总α活性。因此,具有总α活性的供水设施>;15 pCi/L应利用铀的放射化学分析来最准确地计算调整后的总α活性。
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引用次数: 0
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AWWA water science
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