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Interlaboratory Performance Study of Cyanobacteria DNA Reference Materials Using a qPCR Format for Monitoring Cyanobacterial Blooms 使用qPCR格式监测蓝藻华的蓝藻DNA参考物质的实验室间性能研究
IF 1.8 Pub Date : 2025-03-10 DOI: 10.1002/aws2.70018
Leonardo B. Pinheiro, Mark Van Asten, Luminita Antin, Hunter Adams, Judy Y. Qiu, Mary Robinson, Suzane DeLorenzo, Robert Holmes, Megan Hurd, Rueyjing Tang, Kale Clausen, Justin Seikel, Rahana Sudhi, Paul Wright, Konstanze Steiner, Anne Gérard, Somanath Bhat, Anna Baoutina, Kerry Emslie

Digital PCR (dPCR) has increasingly been used as a primary measurement method for the characterization of nucleic acid reference materials. Nucleic acid reference materials are particularly useful when used for the validation and calibration of quantitative PCR (qPCR). In this study, we describe the development and characterization of Cyanobacteria DNA reference materials (RM) using dPCR. An international interlaboratory study involving 14 laboratories was conducted using the Cyanobacteria DNA RM in combination with a lyophilized PCR reagent designed for the monitoring of Cyanobacteria bloom events. Of the 55 scored study results obtained using qPCR-based techniques, 62% were within the 8% relative expanded uncertainty based on dPCR measurements, while 100% of the study results returned satisfactory z scores calculated using a set performance coefficient of variation equivalent to one Ct value. The study participants' results indicate that the cyanobacteria DNA RM is fit for the purpose of method validation and quality control of the qPCR format used for monitoring toxic cyanobacteria algae bloom events. Most importantly, the study results demonstrated that the use of standardized reagents combined with highly characterized nucleic acid RMs allows qPCR-based DNA quantification technology to reach levels of accuracy and reproducibility comparable to those achieved with digital PCR technology.

数字PCR (dPCR)已越来越多地被用作核酸标准物质表征的主要测量方法。当用于定量PCR (qPCR)的验证和校准时,核酸参考物质特别有用。在这项研究中,我们描述了蓝藻DNA参考物质(RM)的开发和使用dPCR表征。一项涉及14个实验室的国际实验室间研究使用蓝藻DNA RM结合冻干PCR试剂设计用于监测蓝藻华事件。在使用基于qpcr的技术获得的55个评分研究结果中,62%在基于dPCR测量的8%的相对扩展不确定性范围内,而100%的研究结果返回满意的z分数,使用相当于一个Ct值的一组性能变异系数计算。研究参与者的结果表明,蓝藻DNA RM适合用于监测有毒蓝藻华事件的qPCR格式的方法验证和质量控制。最重要的是,研究结果表明,使用标准化试剂结合高度表征的核酸RMs,基于qpcr的DNA定量技术可以达到与数字PCR技术相当的准确性和可重复性。
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引用次数: 0
Robustness and Related Concepts for Climate Adaptation in Drinking Water Treatment Systems 饮用水处理系统气候适应的鲁棒性和相关概念
IF 1.8 Pub Date : 2025-02-20 DOI: 10.1002/aws2.70017
Kirti S. Nemani, Sigrid Peldszus, Peter M. Huck

Climate change intensifies extreme weather events, potentially posing significant challenges to the quality and quantity of surface water available for drinking water treatment. Quantifying and substantiating a treatment system's capacity and vulnerability in handling a range of raw water conditions is crucial for preparing for future climate scenarios. Concepts like resilience and reliability and related tools have been applied to drinking water treatment plants (DWTPs), but often fail to capture the operational boundaries of treatment processes. Robustness offers a complementary approach, focusing on the range of conditions a system can effectively manage, thereby laying the foundation for improving the system and thus bridging a critical gap in adaptation strategies. This review examines the interconnections between robustness, resilience, reliability, risk, and vulnerability, providing tailored definitions for DWTPs. It also introduces visual diagrams to further illustrate their link and collective role in climate adaptation planning.

气候变化加剧了极端天气事件,可能对可用于饮用水处理的地表水的质量和数量构成重大挑战。量化和证实处理系统在处理一系列原水条件方面的能力和脆弱性,对于为未来的气候情景做好准备至关重要。复原力和可靠性等概念以及相关工具已应用于饮用水处理厂,但往往无法捕捉处理过程的操作边界。稳健性提供了一种补充方法,侧重于系统能够有效管理的条件范围,从而为改进系统奠定基础,从而弥补适应战略中的关键差距。本文考察了鲁棒性、弹性、可靠性、风险和脆弱性之间的相互联系,并为dwtp提供了量身定制的定义。它还引入了可视化图表,以进一步说明它们在气候适应规划中的联系和集体作用。
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引用次数: 0
Lithium Removal From Drinking Water 从饮用水中去除锂
IF 1.8 Pub Date : 2025-02-10 DOI: 10.1002/aws2.70016
Asher E. Keithley, Page Jordan, Christy Muhlen, Matthew Pinelli, Darren A. Lytle

Lithium (Li) is listed on the U.S. Environmental Protection Agency's fifth Contaminant Candidate List (CCL 5), and its occurrence in drinking water is being quantified under the fifth Unregulated Contaminant Monitoring Rule (UCMR5). Little information is available on its removal from drinking water. The current study evaluated 19 historical pilot- and full-scale studies and conducted sampling at 13 water treatment plants. The 32 sites included 3 surface water and 29 groundwater sources, and 8 treatment process categories. Conventional surface water treatment, adsorptive media, biological aerobic groundwater treatment, and manganese removal filters were not effective at removing Li. Cation exchange sometimes achieved Li removal, but removals were inconsistent. Lime softening often removed 11%–54% Li, but treated water Li typically was > 10 μg/L. RO removed > 90% of Li, although finished water concentrations depended on blending rates. This study fills a critical gap in evaluating Li treatability through the existing water treatment infrastructure.

锂(Li)被列入美国环境保护署的第五种污染物候选清单(CCL 5),其在饮用水中的出现情况正在根据第五种不受管制的污染物监测规则(UCMR5)进行量化。关于将其从饮用水中去除的信息很少。目前的研究评估了19个历史试点和全面研究,并在13个水处理厂进行了采样。32个场址包括地表水3个,地下水29个,处理工艺类别8个。常规地表水处理、吸附介质、地下水生物好氧处理和除锰过滤器均不能有效去除锂。阳离子交换有时能脱除锂,但脱除效果不一致。石灰软化可去除11% ~ 54%的锂,但处理后的水锂一般为10 μg/L。虽然成品水的浓度取决于混合率,但反渗透去除了90%的锂。本研究填补了通过现有水处理基础设施评估锂可处理性的关键空白。
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引用次数: 0
Automated Acquisition of Historical Water Distribution Pipe Repair Location Data 历史配水管维修位置数据的自动获取
IF 1.8 Pub Date : 2025-01-29 DOI: 10.1002/aws2.70015
Charles Scawthorn, Joseph McGlinchy, Keith Porter

We report an innovative method of extracting water distribution network (WDN) historical repair location data from images of paper repair data maps, to provide usable geo-referenced digitally formatted data. For most water utilities, repair location data typically fall into two eras: pre- and post-GIS, approximately corresponding to pre- and post-2000. Automated conversion of pre-2000 paper maps to a geo-referenced digital format provides additional data to clarify trends in pipe repair causative factors, such as material defects, corrosive or creeping soils, and traffic. We applied the methodology to more than 3,000 maps of the Los Angeles Department of Water and Power WDN, thereby extending the record of repairs backward from 2000 to 1975, almost doubling the number of repair records. The methodology's value, when using the resulting data for analysis, lies in the following: (a) large volumes of hard copy data can now be acquired in an automated manner, saving significant time and effort, (b) specific repair locations are accurately captured, resulting in (c) more reliable, confident, analyses, and results, (d) ongoing problem areas, due to corrosive or creeping soils for example, can be more specifically understood.

我们报告了一种从纸质维修数据图图像中提取供水网络(WDN)历史维修位置数据的创新方法,以提供可用的地理参考数字格式数据。对于大多数水务公司,维修位置数据通常分为两个时代:前和后gis,大约对应于前和后2000年。将2000年以前的纸质地图自动转换为地理参考的数字格式,提供了额外的数据,以阐明管道维修原因的趋势,例如材料缺陷、腐蚀或蠕动的土壤以及交通。我们将该方法应用于洛杉矶水电局WDN的3000多张地图,从而将维修记录从2000年追溯至1975年,几乎将维修记录的数量增加了一倍。当使用结果数据进行分析时,该方法的价值在于:(a)现在可以以自动化的方式获取大量的硬拷贝数据,节省了大量的时间和精力;(b)准确捕获特定的修复位置,从而产生(c)更可靠、更自信的分析和结果;(d)可以更具体地了解由于腐蚀或蠕动土壤等原因导致的持续问题区域。
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引用次数: 0
Understanding the Cost of Basic Drinking Water Services in the United States: A National Assessment 了解美国基本饮用水服务的成本:一项国家评估
IF 1.8 Pub Date : 2025-01-23 DOI: 10.1002/aws2.70014
Sara Hughes, Christine J. Kirchhoff, Michelle Lee, David Switzer

The cost of basic drinking water services has implications for affordability, investment capacity, and public health. The fragmentation of drinking water services in the United States makes it difficult to reliably track and compare what customers pay for basic drinking water services. This paper uses a new, national dataset to examine the social, political, environmental, and institutional drivers of the cost of basic drinking water services, measured as the cost to households of 6000 gal of water per month. We find basic drinking water service costs vary widely across the United States. Costs are generally higher in smaller and more liberal cities and lower in places that rely on groundwater sources. Our findings provide a unique national perspective on variation in, and drivers of, the cost of basic water services and can inform efforts to improve the affordability, accessibility, and quality of drinking water services in the United States.

基本饮用水服务的成本对负担能力、投资能力和公共卫生都有影响。美国饮用水服务的分散性使得很难可靠地跟踪和比较客户为基本饮用水服务支付的费用。本文使用一个新的国家数据集来考察基本饮用水服务成本的社会、政治、环境和制度驱动因素,以家庭每月6000加仑水的成本来衡量。我们发现,美国各地的基本饮用水服务成本差异很大。在更自由的小城市,成本通常更高,而在依赖地下水资源的地方,成本则更低。我们的研究结果为基本供水服务成本的变化和驱动因素提供了一个独特的国家视角,并可以为提高美国饮用水服务的可负担性、可及性和质量提供信息。
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引用次数: 0
Method Development, Interlaboratory Comparison, and Occurrence Study for 19 Taste and Odor Compounds by Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry 用固相微萃取-气相色谱-质谱法测定19种味觉和气味化合物的方法发展、实验室间比较和发生研究
IF 1.8 Pub Date : 2025-01-07 DOI: 10.1002/aws2.70013
Hunter Adams, Sam Reeder, Katie Kohoutek, Christiane Hoppe-Jones, Susheera Pochiraju, Mark Southard, Keisuke Ikehata, Carlos A. Espindola Jr, Andrea M. Dietrich, Gary A. Burlingame, Daniel K. Nix, Ruth Marfil-Vega, Terry Jeffers, I. H. (Mel) Suffet, Michelle Ashman, Kandé Duncan, Eduardo Morales, William C. Lipps

Public water systems (PWSs) need robust taste and odor (T&O) methods for a diverse range of compounds to proactively monitor their systems from source to tap and make informed treatment decisions. In this study, Standard Method 6040D T&O compounds by solid-phase microextraction gas chromatography–mass spectrometry was revised to include 19 T&O compounds with various odor descriptors including earthy, musty, grassy, woody, fishy, septic, fruity, and sweet. An interlaboratory comparison was performed to determine method accuracy, precision, reproducibility, and ruggedness. Three laboratories achieved passing quality control (QC) acceptance criteria for all 19 compounds, and one laboratory achieved passing QC acceptance criteria for 14 compounds. In this article, occurrence data and method applications are also discussed, which will allow PWSs to monitor diverse classes of T&O compounds and make informed, proactive treatment decisions to maintain high aesthetic quality for their customers.

公共水系统(PWSs)需要强大的味道和气味(T&;O)方法来检测各种化合物,从源头到水龙头主动监测其系统,并做出明智的处理决策。在本研究中,通过固相微萃取气相色谱-质谱法对标准方法6040D t&o化合物进行了修订,以包括19种具有不同气味描述的t&o化合物,包括泥土味、霉味、草味、木味、鱼味、化脓味、水果味和甜味。进行实验室间比较以确定方法的准确性、精密度、重现性和坚固性。3个实验室的19种化合物全部达到了质量控制合格标准,1个实验室的14种化合物达到了质量控制合格标准。在本文中,还讨论了发生数据和方法应用,这将允许PWSs监测不同类别的T&;O化合物,并做出明智的、主动的处理决策,以保持其客户的高美学质量。
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引用次数: 0
Mixed Adsorbents: Synergic Effects Improve Problematic Compounds Removal During Drinking Water Treatment 混合吸附剂:协同效应改善饮用水处理过程中问题化合物的去除
IF 1.8 Pub Date : 2025-01-07 DOI: 10.1002/aws2.70011
Katerina Sichrova, Lenka Cermakova, Katerina Novotna, Lenka Pivokonska, Veronika Zustakova, Martin Pivokonsky

This study investigated the adsorption using mixed adsorbents comprising different proportions of commercially available granular activated carbons (GACs) and compared their performance to single GACs for removing algal organic matter (AOM) and pesticides. In addition to diverse GAC ratios, the effects of adsorbent dosage and solution pH were investigated for AOM, pesticides, and an AOM-pesticide mixture. The mixed adsorbents can achieve overall higher removal of AOM and pesticides in a mixture. Surprisingly, even the removal of single adsorbates increased when mixed adsorbents were used. The AOM removal increased from 25%–29% using single GACs to 44% using a suitable adsorbent mixture; similarly, the pesticide removal increased from 52%–67% to 74%. The adsorbent ratio was the key influential factor. Additionally, the pH value also significantly affected the adsorption. Using a mixture of different adsorbents is a promising method for treating drinking water.

研究了不同比例的市售颗粒活性炭(GACs)混合吸附剂的吸附性能,并比较了它们与单一颗粒活性炭对藻类有机物(AOM)和农药的去除效果。除了不同的GAC比例外,还研究了吸附剂用量和溶液pH对AOM、农药和AOM-农药混合物的影响。混合吸附剂可以在混合物中实现更高的AOM和农药的整体去除。令人惊讶的是,当使用混合吸附剂时,即使是单一吸附剂的去除率也增加了。对AOM的去除率从单一GACs的25% ~ 29%提高到合适的吸附混合物的44%;同样,农药去除率从52%-67%增加到74%。吸附剂配比是影响吸附效果的关键因素。此外,pH值对吸附也有显著影响。使用不同吸附剂的混合物是一种很有前途的处理饮用水的方法。
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引用次数: 0
Pilot-Scale Analysis of Stagnation and Flushing in Premise Plumbing 住宅水管堵塞和冲水的中试分析
IF 1.8 Pub Date : 2025-01-06 DOI: 10.1002/aws2.70012
Derek Hogue, McKenzie Steele, Treavor H. Boyer

Research has demonstrated that water quality degrades in commercial and institutional (C&I) building premise plumbing leading to increased risk to consumers. This study aimed to bridge the gap between real premise plumbing systems and theory by using a pilot scale pipe rig representative of C&I premise plumbing. The research examined changes in key water quality parameters, including chlorine, copper, trihalomethanes (THMs), and cellular ATP (cATP) across different flushing and stagnation conditions. Results indicated significant degradation during periods of stagnation found in real premise plumbing, with reductions in chlorine levels and increases in copper and THM concentrations. Conversely, flushing effectively renewed water quality, though the extent varied with system size and flow dynamics. Correlations were found between key water quality variables. The findings emphasize the need for strategic water management practices to mitigate risks associated with poor water quality in building plumbing systems.

研究表明,在商业和机构(C&;I)建筑前提管道中,水质会下降,导致消费者的风险增加。本研究旨在通过使用C&;I前提管道的中试规模管道钻机,弥合实际前提管道系统与理论之间的差距。该研究检查了关键水质参数的变化,包括氯、铜、三卤甲烷(THMs)和细胞ATP (cATP)在不同冲洗和停滞条件下的变化。结果表明,在实际住宅管道中发现的停滞期间,氯水平降低,铜和THM浓度增加,显著降解。相反,冲洗有效地更新了水质,尽管程度随系统规模和流动动力学而变化。关键水质变量之间存在相关性。研究结果强调需要战略性的水管理实践,以减轻建筑管道系统中与水质差有关的风险。
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引用次数: 0
Impact of flocculated and softened particles on UV254 inactivation of indigenous spores 絮凝和软化颗粒对本地孢子UV254失活的影响
IF 1.8 Pub Date : 2024-12-22 DOI: 10.1002/aws2.70010
Judith Straathof, Zuzana Bohrerova, Natalie M. Hull

US regulatory ultraviolet (UV) disinfection credit is typically granted when turbidity is ≤1 NTU. However, studies show turbidity does not always correlate well with UV dose responses. This study examined the impact of worst-case high turbidity scenarios at drinking water treatment plants on UV254 inactivation of indigenous spores from unfiltered source water and unsettled flocculation and softening steps. Flocculated water (turbidity = 6.49–164 NTU) had the lowest dose response with a significantly lower Geeraerd-tail maximum inactivation rate (kmax = 0.021 cm2/mJ) and higher residual population density (Nres = 7.081 SFU/mL). Raw source water (kmax = 0.027 cm2/mJ, Nres = 1.168 SFU/mL, turbidity = 0.978–215 NTU) and softened water (kmax = 0.030 cm2/mJ, Nres = 0.216 SFU/mL, turbidity = 318–495 NTU) had similar dose responses despite significantly different water quality. Particle size and the degree of particle-associated spores best explained the differences in dose responses. Almost all spores were associated with flocculated particles instead of free-floating, which increased tailing and negatively impacted UV inactivation. Based on regulatory reduction equivalent dose bias factors and UV sensitivities of spiked Bacillus subtilis spores, Cryptosporidium would be 4-log inactivated in these raw, flocculated, or softened waters if UV transmission were ≥65%, 90%, or 80%, respectively, even though turbidity was grossly >1 NTU. Depending on particle characteristics, partial inactivation credit when turbidity is >1 NTU should be considered to avoid high-tier violations while still protecting public health.

当浊度≤1 NTU时,通常授予美国监管紫外线(UV)消毒信用。然而,研究表明,浊度并不总是与紫外线剂量反应密切相关。本研究考察了饮用水处理厂最坏情况下的高浊度情况对未过滤水源水中原生孢子UV254失活的影响,以及不稳定的絮凝和软化步骤。混凝水(浊度为6.49 ~ 164 NTU)的剂量响应最低,其最大灭活率(kmax = 0.021 cm2/mJ)显著降低,残留种群密度(Nres = 7.081 SFU/mL)较高。原水(kmax = 0.027 cm2/mJ, Nres = 1.168 SFU/mL,浊度= 0.978 ~ 215 NTU)和软化水(kmax = 0.030 cm2/mJ, Nres = 0.216 SFU/mL,浊度= 318 ~ 495 NTU)尽管水质差异显著,但剂量反应相似。颗粒大小和颗粒相关孢子的程度最好地解释了剂量反应的差异。几乎所有孢子都与絮凝颗粒结合,而不是自由漂浮的颗粒,这增加了尾矿,并对紫外线灭活产生了不利影响。根据调控减少等效剂量偏置因子和加标枯草芽孢杆菌孢子的紫外线敏感性,如果紫外线透过率分别≥65%、90%或80%,即使浊度约为1 NTU,隐孢子虫在这些未经处理、絮凝或软化的水中的失活率为4-log。根据颗粒特性,当浊度为1 NTU时,应考虑部分失活信用,以避免高级别违规,同时仍然保护公众健康。
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引用次数: 0
Lithium in drinking water: Review of chemistry, analytical methods, and treatment technologies 饮用水中的锂:化学、分析方法和处理技术综述
IF 1.8 Pub Date : 2024-12-17 DOI: 10.1002/aws2.70009
Julie A. Korak, Philip J. Brandhuber, Joseph E. Goodwill

Lithium was included in the fifth Unregulated Contaminants Monitoring Rule, signaling the Environmental Protection Agency's interest in regulating lithium. Many questions regarding occurrence, health effects, and treatability of lithium exist. This review primarily focuses on the relationship between lithium chemistry and treatability. Sampling indicates nationwide lithium occurrence in drinking water. Yet, lithium is not included in the Integrated Risk Information System, reflecting a lack of censuses regarding its health effects. Aqueous lithium is a monovalent cation with size, charge density, and solubility properties that present treatment challenges. Lithium's growing economic value is stimulating new extraction and isolation technologies, but these may not be transferable to drinking water treatment. Currently, reverse osmosis is the only full-scale drinking water treatment technology that can reliably remove significant levels (>50%) of lithium. Focusing future research efforts on electrodialysis and inorganic ion sieves may yield significant gains in effectiveness and readiness for the drinking water industry.

锂被列入第五项不受管制的污染物监测规则,这标志着环境保护局对监管锂的兴趣。关于锂的发生、健康影响和可治疗性存在许多问题。本文主要综述了锂化学与可治疗性之间的关系。抽样显示全国饮用水中存在锂。然而,锂不包括在综合风险信息系统中,这反映了缺乏关于其健康影响的人口普查。含水锂是一种单价阳离子,其大小、电荷密度和溶解度都给处理带来了挑战。锂日益增长的经济价值刺激了新的提取和分离技术,但这些技术可能无法转移到饮用水处理中。目前,反渗透是唯一能够可靠地去除大量(50%)锂的全面饮用水处理技术。将未来的研究重点放在电渗析和无机离子筛上,可能会在饮用水工业的有效性和准备性方面取得重大进展。
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引用次数: 0
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AWWA water science
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