Clinical and Experimental Dental Research最新文献_第5页

FOXP3+ T Cells—An Emerging Evidence in Periodontitis Therapeutics FOXP3+ T细胞-牙周炎治疗的新证据。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-15 DOI: 10.1002/cre2.70263

Revan Birke Koca Ünsal, Akira Hasuike, Tamer Badawy, Farah Asa'ad, Bruno Špiljak, Monal Yuwanati, Jelena Roganović, Yinli Liu, Carel Brigi, Danilo Milanes Zambrano, Akhilanand Chaurasia

Objective

To review interaction of FOXP3+ regulatory T cells with Th17 cells in determining the progression of periodontitis.

Material and Methodology

Literature review pertaining to FOXP3+ regulatory T cells, Th17 cells, and periodontitis was analyzed. Descriptive summary is presented.

Results

FOXP3+ regulatory T cells (Tregs) play an essential role in maintaining immune homeostasis and modulating inflammatory responses. The balance between Tregs and pro-inflammatory Th17 cells is crucial in determining the progression of periodontitis, a chronic immune-mediated inflammatory disease. While Tregs are responsible for suppressing excessive immune activation and preventing tissue destruction, an imbalance favoring Th17 cells leads to increased osteoclastic activity and alveolar bone loss through IL-17 and RANKL signaling. The inflammatory microenvironment in periodontitis compromises FOXP3+ Treg stability and function, thereby allowing unregulated immune responses that exacerbate periodontal tissue breakdown. Recent studies suggest that strategies aimed at enhancing Treg-mediated immune regulation, such as IL-2 supplementation, all-trans retinoic acid (ATRA), IL-33 administration, and CCL22-mediated recruitment, could mitigate periodontal inflammation and preserve alveolar bone integrity. Furthermore, systemic conditions like diabetes and obesity play a significant role in disrupting Treg function by promoting a pro-inflammatory environment, impairing immune regulation, and exacerbating immune dysregulation. This dysfunction weakens the protective role of Tregs, leading to an intensified inflammatory response that accelerates periodontal tissue destruction and alveolar bone loss.

Conclusion

Understanding the mechanisms governing FOXP3+ Treg stability and their interaction with pathogenic Th17 responses is essential for developing targeted immunomodulatory therapies. Future research should focus not only on selectively expanding Tregs but also on translational strategies such as adoptive Treg transfer and IL-17 inhibition, while carefully balancing efficacy and the risk of systemic immunosuppression.

目的：探讨FOXP3+调节性T细胞与Th17细胞在牙周炎进展中的相互作用。材料和方法：回顾有关FOXP3+调节性T细胞、Th17细胞和牙周炎的文献进行分析。给出了描述性的总结。结果：FOXP3+调节性T细胞（Tregs）在维持免疫稳态和调节炎症反应中发挥重要作用。Tregs和促炎Th17细胞之间的平衡在决定牙周炎（一种慢性免疫介导的炎症性疾病）的进展中至关重要。虽然treg负责抑制过度免疫激活和防止组织破坏，但有利于Th17细胞的不平衡通过IL-17和RANKL信号传导导致破骨细胞活性增加和牙槽骨丢失。牙周炎的炎症微环境会损害FOXP3+ Treg的稳定性和功能，从而导致不受调节的免疫反应，从而加剧牙周组织的破坏。最近的研究表明，旨在增强treg介导的免疫调节的策略，如补充IL-2、全反式维甲酸（ATRA）、IL-33和ccl22介导的招募，可以减轻牙周炎症并保持牙槽骨完整性。此外，糖尿病和肥胖等全身性疾病通过促进促炎环境、损害免疫调节和加剧免疫失调，在破坏Treg功能方面发挥重要作用。这种功能障碍削弱了Tregs的保护作用，导致炎症反应加剧，加速牙周组织破坏和牙槽骨丢失。结论：了解FOXP3+ Treg稳定性的调控机制及其与致病性Th17反应的相互作用，对于开发靶向免疫调节疗法至关重要。未来的研究不仅应关注Treg的选择性扩增，还应关注过继性Treg转移和IL-17抑制等转化策略，同时仔细平衡功效和全身免疫抑制的风险。

{"title":"FOXP3+ T Cells—An Emerging Evidence in Periodontitis Therapeutics","authors":"Revan Birke Koca Ünsal, Akira Hasuike, Tamer Badawy, Farah Asa'ad, Bruno Špiljak, Monal Yuwanati, Jelena Roganović, Yinli Liu, Carel Brigi, Danilo Milanes Zambrano, Akhilanand Chaurasia","doi":"10.1002/cre2.70263","DOIUrl":"10.1002/cre2.70263","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To review interaction of FOXP3+ regulatory T cells with Th17 cells in determining the progression of periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methodology</h3>\u0000 \u0000 <p>Literature review pertaining to FOXP3+ regulatory T cells, Th17 cells, and periodontitis was analyzed. Descriptive summary is presented.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>FOXP3+ regulatory T cells (Tregs) play an essential role in maintaining immune homeostasis and modulating inflammatory responses. The balance between Tregs and pro-inflammatory Th17 cells is crucial in determining the progression of periodontitis, a chronic immune-mediated inflammatory disease. While Tregs are responsible for suppressing excessive immune activation and preventing tissue destruction, an imbalance favoring Th17 cells leads to increased osteoclastic activity and alveolar bone loss through IL-17 and RANKL signaling. The inflammatory microenvironment in periodontitis compromises FOXP3+ Treg stability and function, thereby allowing unregulated immune responses that exacerbate periodontal tissue breakdown. Recent studies suggest that strategies aimed at enhancing Treg-mediated immune regulation, such as IL-2 supplementation, all-trans retinoic acid (ATRA), IL-33 administration, and CCL22-mediated recruitment, could mitigate periodontal inflammation and preserve alveolar bone integrity. Furthermore, systemic conditions like diabetes and obesity play a significant role in disrupting Treg function by promoting a pro-inflammatory environment, impairing immune regulation, and exacerbating immune dysregulation. This dysfunction weakens the protective role of Tregs, leading to an intensified inflammatory response that accelerates periodontal tissue destruction and alveolar bone loss.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Understanding the mechanisms governing FOXP3+ Treg stability and their interaction with pathogenic Th17 responses is essential for developing targeted immunomodulatory therapies. Future research should focus not only on selectively expanding Tregs but also on translational strategies such as adoptive Treg transfer and IL-17 inhibition, while carefully balancing efficacy and the risk of systemic immunosuppression.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Dental Anatomy: An Ex Vivo Comparative Study Between Photon-Counting Detector CT and Cone-Beam CT 探索口腔解剖学：光子计数检测器CT与锥束CT的离体比较研究。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-15 DOI: 10.1002/cre2.70266

Adib Al-Haj Husain, Fabian Benz, Victor Mergen, Silvio Valdec, Sebastian Winklhofer, Hatem Alkadhi, Harald Essig, Thomas Frauenfelder, Rubens Spin-Neto, Bernd Stadlinger

Objectives

The aim of this ex vivo study was to assess the performance of photon-counting detector computed tomography (PCD-CT) compared with cone-beam computed tomography (CBCT) at equivalent radiation doses for detecting and evaluating mandibular and dental anatomical structures in porcine cadavers.

Material and Methods

This intermodal comparative study evaluated imaging protocols at three radiation dose levels (high: 360 μSv, standard: 145 μSv, low: 20 μSv) in six porcine cadaver heads, analyzing 12 CBCT and 18 PCD-CT volumes. Two blinded observers assessed image quality, artifact susceptibility, and diagnostic interpretability using a 5-point Likert scale (5 = highest, 1 = lowest). Statistical analysis included descriptive statistics and interobserver reliability, assessed by weighted kappa (κ) analysis.

Results

PCD-CT either matched or outperformed CBCT at standard- and low-dose protocols, demonstrating superior anatomy coverage, density, contrast, and less artifact susceptibility. High- and standard-dose protocols achieved perfect scores 5 (SD = 0). At low-dose levels, PCD-CT showed slightly lower scores but still outperformed CBCT. PCD-CT showed minimal artifacts, with no significant artifacts in high and standard doses. Inter- and intra-reader reliability was higher for PCD-CT (κ: 0.694–1; p < 0.001) compared to CBCT (0.55–0.916; p < 0.001), with the difference being largest at low doses.

Conclusion

PCD-CT outperforms CBCT in diagnostic interpretability and artifact reduction across various radiation dose levels, offering a promising alternative for dentomaxillofacial imaging that aligns with the ALADAIP principle.

目的：本离体研究的目的是评估光子计数检测器计算机断层扫描（PCD-CT）与锥束计算机断层扫描（CBCT）在相同辐射剂量下检测和评估猪尸体下颌骨和牙齿解剖结构的性能。材料和方法：本多式联用比较研究评估了6头猪尸体头部在3种辐射剂量水平（高：360 μSv，标准：145 μSv，低：20 μSv）下的成像方案，分析了12个CBCT和18个PCD-CT体积。两名盲法观察者使用5点李克特量表（5 =最高，1 =最低）评估图像质量、伪影易感性和诊断可解释性。统计分析包括描述性统计和观察者间信度，采用加权kappa （κ）分析评估。结果：在标准和低剂量方案下，PCD-CT匹配或优于CBCT，显示出优越的解剖覆盖范围、密度、对比度和更少的伪影敏感性。高剂量和标准剂量方案的评分为5分（SD = 0）。在低剂量水平下，PCD-CT的得分略低，但仍优于CBCT。PCD-CT显示伪影最小，在高剂量和标准剂量下无明显伪影。结论：在不同辐射剂量水平下，PCD-CT在诊断可解释性和伪影减少方面优于CBCT，为符合ALADAIP原则的牙颌面成像提供了一种有希望的替代方案。

{"title":"Exploring Dental Anatomy: An Ex Vivo Comparative Study Between Photon-Counting Detector CT and Cone-Beam CT","authors":"Adib Al-Haj Husain, Fabian Benz, Victor Mergen, Silvio Valdec, Sebastian Winklhofer, Hatem Alkadhi, Harald Essig, Thomas Frauenfelder, Rubens Spin-Neto, Bernd Stadlinger","doi":"10.1002/cre2.70266","DOIUrl":"10.1002/cre2.70266","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aim of this ex vivo study was to assess the performance of photon-counting detector computed tomography (PCD-CT) compared with cone-beam computed tomography (CBCT) at equivalent radiation doses for detecting and evaluating mandibular and dental anatomical structures in porcine cadavers.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>This intermodal comparative study evaluated imaging protocols at three radiation dose levels (high: 360 μSv, standard: 145 μSv, low: 20 μSv) in six porcine cadaver heads, analyzing 12 CBCT and 18 PCD-CT volumes. Two blinded observers assessed image quality, artifact susceptibility, and diagnostic interpretability using a 5-point Likert scale (5 = highest, 1 = lowest). Statistical analysis included descriptive statistics and interobserver reliability, assessed by weighted kappa (κ) analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>PCD-CT either matched or outperformed CBCT at standard- and low-dose protocols, demonstrating superior anatomy coverage, density, contrast, and less artifact susceptibility. High- and standard-dose protocols achieved perfect scores 5 (SD = 0). At low-dose levels, PCD-CT showed slightly lower scores but still outperformed CBCT. PCD-CT showed minimal artifacts, with no significant artifacts in high and standard doses. Inter- and intra-reader reliability was higher for PCD-CT (κ: 0.694–1; <i>p</i> < 0.001) compared to CBCT (0.55–0.916; <i>p</i> < 0.001), with the difference being largest at low doses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>PCD-CT outperforms CBCT in diagnostic interpretability and artifact reduction across various radiation dose levels, offering a promising alternative for dentomaxillofacial imaging that aligns with the ALADAIP principle.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep Learning Models for Evaluating the Anatomical Relationship Between Posterior Maxillary Teeth and Maxillary Sinus in Panoramic Radiographs 深度学习模型评价上颌后牙与上颌窦在全景x线片上的解剖关系。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-15 DOI: 10.1002/cre2.70264

Akram Fallah, Parisa Soltani, Mojdeh Mehdizadeh, Mostafa Riahi Farsani, Mahsa Moannaei, Seyed Amir Hossein Ourang, Maryam Hossaini, Gianrico Spagnuolo, Carlo Rengo

Objectives

Accurate evaluation of the anatomical relationship between posterior maxillary teeth and the maxillary sinus is critical in dental treatments such as orthodontics, surgery, and implantology. This study investigates the efficacy of deep learning models applied to panoramic images for predicting this anatomical relationship.

Materials and Methods

A total of 300 panoramic images and 1760 cropped slices were collected and converted to PNG format at a resolution of 512 × 512 pixels. Three convolutional neural network architectures—VGG, ResNet, and ResNeXt—were trained and evaluated. The dataset was split into training (80%), validation (10%), and testing (10%) sets. Performance metrics included accuracy, precision, recall, F1 score, ROC-AUC, and confusion matrices.

Results

The VGG model achieved an accuracy, precision, recall, and F1 score of 0.84, with an ROC-AUC of 0.89. ResNet and ResNeXt demonstrated superior performance with all metrics at 0.88 and ROC-AUC values of 0.93 and 0.94, respectively. Both ResNet and ResNeXt showed signs of overfitting after 30–50 epochs, suggesting the need for early stopping or regularization. False positives mainly occurred in second molars falsely classified as contacting the sinus.

Conclusion

Deep learning models, particularly ResNet and ResNeXt, provide reliable assessment of the anatomical relationship between posterior maxillary teeth and the maxillary sinus using panoramic radiographs. These models can serve as efficient diagnostic aids when CBCT is unavailable, improving clinical decision-making in panoramic images.

目的：准确评估上颌后牙与上颌窦之间的解剖关系在正畸、外科和种植等牙科治疗中至关重要。本研究探讨了应用于全景图像的深度学习模型预测这种解剖关系的有效性。材料与方法：收集全景图像300张，裁剪切片1760张，转换为PNG格式，分辨率为512 × 512像素。对vgg、ResNet和resnext三种卷积神经网络架构进行了训练和评估。数据集被分成训练集（80%）、验证集（10%）和测试集（10%）。性能指标包括准确性、精密度、召回率、F1分数、ROC-AUC和混淆矩阵。结果：VGG模型的准确率、精密度、召回率和F1得分为0.84，ROC-AUC为0.89。ResNet和ResNeXt表现出优异的性能，所有指标分别为0.88，ROC-AUC值分别为0.93和0.94。ResNet和ResNeXt在30-50个epoch后都显示出过拟合的迹象，这表明需要尽早停止或正则化。假阳性主要发生在第二磨牙误分类为接触鼻窦。结论：深度学习模型，特别是ResNet和ResNeXt，可以通过全景x线片可靠地评估上颌后牙与上颌窦的解剖关系。这些模型可以作为有效的诊断辅助，当CBCT不可用，提高临床决策的全景图像。

{"title":"Deep Learning Models for Evaluating the Anatomical Relationship Between Posterior Maxillary Teeth and Maxillary Sinus in Panoramic Radiographs","authors":"Akram Fallah, Parisa Soltani, Mojdeh Mehdizadeh, Mostafa Riahi Farsani, Mahsa Moannaei, Seyed Amir Hossein Ourang, Maryam Hossaini, Gianrico Spagnuolo, Carlo Rengo","doi":"10.1002/cre2.70264","DOIUrl":"10.1002/cre2.70264","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Accurate evaluation of the anatomical relationship between posterior maxillary teeth and the maxillary sinus is critical in dental treatments such as orthodontics, surgery, and implantology. This study investigates the efficacy of deep learning models applied to panoramic images for predicting this anatomical relationship.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 300 panoramic images and 1760 cropped slices were collected and converted to PNG format at a resolution of 512 × 512 pixels. Three convolutional neural network architectures—VGG, ResNet, and ResNeXt—were trained and evaluated. The dataset was split into training (80%), validation (10%), and testing (10%) sets. Performance metrics included accuracy, precision, recall, F1 score, ROC-AUC, and confusion matrices.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The VGG model achieved an accuracy, precision, recall, and F1 score of 0.84, with an ROC-AUC of 0.89. ResNet and ResNeXt demonstrated superior performance with all metrics at 0.88 and ROC-AUC values of 0.93 and 0.94, respectively. Both ResNet and ResNeXt showed signs of overfitting after 30–50 epochs, suggesting the need for early stopping or regularization. False positives mainly occurred in second molars falsely classified as contacting the sinus.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Deep learning models, particularly ResNet and ResNeXt, provide reliable assessment of the anatomical relationship between posterior maxillary teeth and the maxillary sinus using panoramic radiographs. These models can serve as efficient diagnostic aids when CBCT is unavailable, improving clinical decision-making in panoramic images.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Will L-PRF Be the Future of Endodontic Microsurgery? A Series of Case Reports L-PRF会是牙髓显微外科的未来吗？一系列的案例报告。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-15 DOI: 10.1002/cre2.70198

Natrah Ahmad Fuad, Panagiotis Pitros, Graeme Brown, Eleni Besi

Objectives

This case series aimed to evaluate the healing potential of apical tissues with large periapical radiolucencies (> 10 mm) after apical microsurgery with L-PRF. The secondary objectives were to evaluate L-PRF's benefits and adverse effects as well as to aid in the development of a clinical protocol.

Materials and Methods

This case series was conducted in accordance with the Preferred Reporting Items for Case Reports in Endodontics (PRICE) 2020 guidelines. Thirteen patients with persistent endodontic infections, unresponsive to nonsurgical root canal treatment/retreatment, were treated at the Restorative and Oral Surgery Departments with endodontic microsurgery. L-PRF preparation followed Choukroun et al. (2001) and the L-PRF 2018 guidelines under the supervision of an experienced consultant. Postoperative follow-up included a phone call at 24 h to assess pain, swelling, and daily functions. Sutures were removed at 7 days, and a 6-month clinical and radiographic review was conducted. The clinical assessment included patient-reported symptoms and extraoral and intraoral examinations. Periapical radiographs were assessed for periapical healing based on Rud et al.'s (1972) radiographic criteria. Radiographs were reviewed by one clinician under standardized conditions.

Results

Histopathological analyses identified 76.9% (n = 10) radicular cysts and 23.0% (n = 3) periapical granulomas from the 13 cases. At the 6-month review, 76.9% (n = 10) showed incomplete healing, 15.4% (n = 2) demonstrated complete healing, and 7.7% (n = 1) had incomplete healing at 4 months. All patients remained asymptomatic with no reported complaints. Radiographic assessments showed a significant reduction in the size of periapical radiolucency in all cases. At 24 h, 69.2% (n = 9) reported no pain, while mild pain was noted in 15.4% (n = 2). Swelling was observed in 69.2% (n = 9) and absent in 15.4% (n = 2), with missing records for 15.4% (n = 2).

Conclusion

L-PRF appears beneficial in endodontic microsurgery. However, larger, low-bias studies with extended follow-up periods are needed for definitive conclusions on its application.

目的：本病例系列旨在评估L-PRF根尖显微手术后根尖周围大放射率（bbb10 mm）的根尖组织的愈合潜力。次要目标是评估L-PRF的益处和不良反应，以及帮助制定临床方案。材料和方法：本病例系列按照牙髓学病例报告的首选报告项目（PRICE） 2020指南进行。13例对非手术根管治疗/再治疗无反应的持续性牙髓感染患者，在修复和口腔外科接受牙髓显微手术治疗。L-PRF的编制遵循Choukroun等人（2001）和L-PRF 2018指南，并在经验丰富的顾问的监督下进行。术后随访包括24小时电话评估疼痛、肿胀和日常功能。第7天拆除缝线，进行6个月的临床和影像学检查。临床评估包括患者报告的症状以及口外和口内检查。根据Rud等人（1972）的放射学标准，对根尖周愈合进行评估。x线片由一名临床医生在标准化条件下审查。结果：经组织病理学分析，13例患者中根状囊肿占76.9% (n = 10)，根尖周围肉芽肿占23.0% （n = 3）。在6个月的回顾中，76.9% （n = 10）显示不完全愈合，15.4% （n = 2）显示完全愈合，7.7% （n = 1）在4个月时显示不完全愈合。所有患者均无症状，无报告的投诉。放射学评估显示所有病例根尖周放射透光度明显减小。24小时时，69.2% （n = 9）报告无疼痛，15.4% （n = 2）报告轻度疼痛。69.2% （n = 9）患者出现肿胀，15.4% （n = 2）患者无肿胀，15.4% （n = 2）患者无肿胀记录。结论：L-PRF对牙髓显微手术有益。然而，需要更大的、低偏倚的、延长随访期的研究来得出其应用的明确结论。

{"title":"Will L-PRF Be the Future of Endodontic Microsurgery? A Series of Case Reports","authors":"Natrah Ahmad Fuad, Panagiotis Pitros, Graeme Brown, Eleni Besi","doi":"10.1002/cre2.70198","DOIUrl":"10.1002/cre2.70198","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This case series aimed to evaluate the healing potential of apical tissues with large periapical radiolucencies (> 10 mm) after apical microsurgery with L-PRF. The secondary objectives were to evaluate L-PRF's benefits and adverse effects as well as to aid in the development of a clinical protocol.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This case series was conducted in accordance with the Preferred Reporting Items for Case Reports in Endodontics (PRICE) 2020 guidelines. Thirteen patients with persistent endodontic infections, unresponsive to nonsurgical root canal treatment/retreatment, were treated at the Restorative and Oral Surgery Departments with endodontic microsurgery. L-PRF preparation followed Choukroun et al. (2001) and the L-PRF 2018 guidelines under the supervision of an experienced consultant. Postoperative follow-up included a phone call at 24 h to assess pain, swelling, and daily functions. Sutures were removed at 7 days, and a 6-month clinical and radiographic review was conducted. The clinical assessment included patient-reported symptoms and extraoral and intraoral examinations. Periapical radiographs were assessed for periapical healing based on Rud et al.'s (1972) radiographic criteria. Radiographs were reviewed by one clinician under standardized conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Histopathological analyses identified 76.9% (<i>n</i> = 10) radicular cysts and 23.0% (<i>n</i> = 3) periapical granulomas from the 13 cases. At the 6-month review, 76.9% (<i>n</i> = 10) showed incomplete healing, 15.4% (<i>n</i> = 2) demonstrated complete healing, and 7.7% (<i>n</i> = 1) had incomplete healing at 4 months. All patients remained asymptomatic with no reported complaints. Radiographic assessments showed a significant reduction in the size of periapical radiolucency in all cases. At 24 h, 69.2% (<i>n</i> = 9) reported no pain, while mild pain was noted in 15.4% (<i>n</i> = 2). Swelling was observed in 69.2% (<i>n</i> = 9) and absent in 15.4% (<i>n</i> = 2), with missing records for 15.4% (<i>n</i> = 2).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>L-PRF appears beneficial in endodontic microsurgery. However, larger, low-bias studies with extended follow-up periods are needed for definitive conclusions on its application.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Study on the Fracture Resistance of CAD/CAM–Fabricated Single-Piece Post-Crowns CAD/ cam单件桩冠抗断裂性能的比较研究。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-15 DOI: 10.1002/cre2.70247

Ali Erdem, Mehmet Selim Bilgin, Ibrahim Ersoy, Erhan Dilber, Ebru Nur Işık, Tan Fırat Eyüboğlu, Mutlu Özcan

Objectives

Recently, CAD/CAM materials have become popular in dental clinics; however, information about their fracture resistance as post-crowns remains limited. This study compared the initial fracture resistance of potential single-piece post-crown materials made with CAD/CAM milling to sound teeth.

Materials and Methods

Fifty freshly extracted, non-carious human central incisor teeth underwent endodontic treatment. The roots were then randomly divided into five groups based on the post systems: the control group included teeth filled only with gutta-percha. Monoblock post-crowns were made using four different systems for the other groups: LDS (IPS e.max CAD®, lithium disilicate glass-ceramic), YTZP (inCoris ZI®, Yttrium tetragonal zirconia polycrystal), RNC (Lava Ultimate®, resin-based nanoceramic), and PICN (VITA Enamic®, polymer-infiltrated ceramic network). The post-crowns were cemented with resin cement and tested with a universal testing machine at a crosshead speed of 1.0 mm/min. Data analysis used one-way ANOVA and multiple comparison post-hoc Tukey tests (α = 0.05).

Results

Significant differences were found between the groups (p < 0.001). The control group exhibited the highest fracture resistance (749.25 ± 225.02 N). YTZP showed similar resistance to the control (p = 0.99) and LDS (447.28 ± 168.72 N, p = 0.081), but was significantly higher than RNC (343.79 ± 157.08 N, p = 0.0051) and PICN (348.78 ± 157.44 N, p = 0.0059). LDS, RNC, and PICN did not differ significantly. YTZP experienced more non-repairable fractures (5/10), while PICN predominantly failed in a repairable manner (9/10).

Conclusions

All CAD/CAM post-crowns exceeded functional loads for the anterior region. YTZP and LDS demonstrated greater strength, whereas PICN and RNC, despite being weaker, favored repairable failures—highlighting the importance of balancing strength and clinical manageability.

目的：近年来，CAD/CAM材料在牙科诊所得到广泛应用；然而，关于它们作为后冠抗骨折性的信息仍然有限。本研究比较了采用CAD/CAM铣削技术制作的潜在单件后冠材料的初始抗断裂能力。材料与方法：对50颗新鲜拔牙的无龋人中门牙进行根管治疗。然后根据桩系统将牙根随机分为五组：对照组包括只填充杜仲胶的牙齿。其他组使用四种不同的体系制作单块桩冠：LDS （IPS e.max CAD®，二硅酸锂玻璃陶瓷），YTZP （inCoris ZI®，钇四方氧化锆多晶），RNC （Lava Ultimate®，树脂基纳米陶瓷）和PICN （VITA Enamic®，聚合物渗透陶瓷网络）。桩冠采用树脂水泥胶结，在万能试验机上以1.0 mm/min的十字速度进行测试。数据分析采用单因素方差分析和多重比较事后Tukey检验（α = 0.05）。结果：两组间存在显著差异(p)。结论：所有CAD/CAM后冠均超过前牙区功能负荷。YTZP和LDS表现出更强的强度，而PICN和RNC尽管较弱，但倾向于可修复的失败-强调平衡强度和临床可管理性的重要性。

{"title":"A Comparative Study on the Fracture Resistance of CAD/CAM–Fabricated Single-Piece Post-Crowns","authors":"Ali Erdem, Mehmet Selim Bilgin, Ibrahim Ersoy, Erhan Dilber, Ebru Nur Işık, Tan Fırat Eyüboğlu, Mutlu Özcan","doi":"10.1002/cre2.70247","DOIUrl":"10.1002/cre2.70247","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Recently, CAD/CAM materials have become popular in dental clinics; however, information about their fracture resistance as post-crowns remains limited. This study compared the initial fracture resistance of potential single-piece post-crown materials made with CAD/CAM milling to sound teeth.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Fifty freshly extracted, non-carious human central incisor teeth underwent endodontic treatment. The roots were then randomly divided into five groups based on the post systems: the control group included teeth filled only with gutta-percha. Monoblock post-crowns were made using four different systems for the other groups: LDS (IPS e.max CAD®, lithium disilicate glass-ceramic), YTZP (inCoris ZI®, Yttrium tetragonal zirconia polycrystal), RNC (Lava Ultimate®, resin-based nanoceramic), and PICN (VITA Enamic®, polymer-infiltrated ceramic network). The post-crowns were cemented with resin cement and tested with a universal testing machine at a crosshead speed of 1.0 mm/min. Data analysis used one-way ANOVA and multiple comparison post-hoc Tukey tests (<i>α</i> = 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Significant differences were found between the groups (<i>p</i> < 0.001). The control group exhibited the highest fracture resistance (749.25 ± 225.02 N). YTZP showed similar resistance to the control (<i>p</i> = 0.99) and LDS (447.28 ± 168.72 N, <i>p</i> = 0.081), but was significantly higher than RNC (343.79 ± 157.08 N, <i>p</i> = 0.0051) and PICN (348.78 ± 157.44 N, <i>p</i> = 0.0059). LDS, RNC, and PICN did not differ significantly. YTZP experienced more non-repairable fractures (5/10), while PICN predominantly failed in a repairable manner (9/10).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>All CAD/CAM post-crowns exceeded functional loads for the anterior region. YTZP and LDS demonstrated greater strength, whereas PICN and RNC, despite being weaker, favored repairable failures—highlighting the importance of balancing strength and clinical manageability.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Raman Spectroscopy and Micro-Indentation to Micro-Map the Path and Boundary of NaOCI-Induced Dentine Collagen Changes in an Ex-Vivo Root Canal Irrigation Model 应用拉曼光谱和微压痕技术对离体根管灌洗模型中naoci诱导的牙本质胶原蛋白变化的路径和边界进行微图绘制。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-15 DOI: 10.1002/cre2.70262

Yuan Ng, Michele Recchia, Cristina Pereira, Graham Palmer, Laurent Bozec, Kishor Gulabivala

Objectives

To apply Raman spectroscopy and micro-indentation to micro-map the path and boundary of NaOCl-induced dentine collagen changes in an ex-vivo root canal irrigation model.

Material and Methods

Root canals of extracted single-rooted teeth were prepared and irrigated with NaOCl or saline. Four teeth (NaOCl = 3; saline = 1) embedded in epoxy resin and sectioned transversely into discs were Raman-analyzed on coronal surfaces from inter- and intra-tubular dentine in 4 quadrants over 12, 24, and 48 min-acquisition times. Eight additional teeth stratified by root maturity, irrigated with NaOCl (n = 7) or saline (n = 1), sectioned transversely and then embedded, were Raman-analyzed on apical surfaces at 18 equidistant (50 μm) points/quadrant for Amide bands. Micro-indentation of the corresponding facing sectioned surface was correlated with Amide band changes. Generalized linear and non-linear regression models were used for data analysis.

Results

Spectral quality at 24/48 min was similar and better than at 12 min. Inter-tubular but not intra-tubular spectra were masked by fluorescence. Spectral features near the canal lumen ( < 500 μm) showed more significant collagen alteration and varied by tooth/quadrant but decreasingly towards the cemento-dentinal junction (CDJ) without a clear boundary. Significant (p < 0.0001) changes in Amide I/III bands up to 300 μm from the canal and were accompanied by deeper corresponding indentations upto 200 μm. Canal instrumentation had a significant (p < 0.0001) effect on both Amide-I and Amide-III bands.

Conclusions

NaOCl altered dentinal collagen and reduced microhardness but varied with quadrants/teeth, without a definable boundary; collagen changes were obvious within 300 μm of the canal and microhardness changes within 200 μm but evident to a decreasing extent up to the CDJ.

目的：应用拉曼光谱和微凹痕技术对naocl诱导离体根管灌洗模型牙本质胶原蛋白变化的路径和边界进行微定位。材料与方法：拔出的单根牙根管预备，用NaOCl或生理盐水冲洗。四颗牙（NaOCl = 3，生理盐水= 1）包埋在环氧树脂中，横向切成盘状，在4个象限的冠状面上进行拉曼分析，分别在12、24和48 min采集时间内。另外8颗牙按根成熟度分层，用NaOCl （n = 7）或生理盐水（n = 1）冲洗，横向切片后嵌入，在18个等距（50 μm）点/象限处的根尖表面进行拉曼分析，检测酰胺带。对应面切面的微压痕与酰胺带的变化相关。采用广义线性和非线性回归模型进行数据分析。结果：24/48 min时的光谱质量与12 min时相似且更好。荧光可掩盖管间光谱，而不掩盖管内光谱。结论：NaOCl改变了牙本质胶原蛋白，降低了显微硬度，但随着象限/牙齿的不同而不同，没有明确的边界；胶原蛋白在距离牙管300 μm范围内变化明显，显微硬度在200 μm范围内变化明显，但在CDJ范围内明显降低。

{"title":"Application of Raman Spectroscopy and Micro-Indentation to Micro-Map the Path and Boundary of NaOCI-Induced Dentine Collagen Changes in an Ex-Vivo Root Canal Irrigation Model","authors":"Yuan Ng, Michele Recchia, Cristina Pereira, Graham Palmer, Laurent Bozec, Kishor Gulabivala","doi":"10.1002/cre2.70262","DOIUrl":"10.1002/cre2.70262","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To apply Raman spectroscopy and micro-indentation to micro-map the path and boundary of NaOCl-induced dentine collagen changes in an ex-vivo root canal irrigation model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Root canals of extracted single-rooted teeth were prepared and irrigated with NaOCl or saline. Four teeth (NaOCl = 3; saline = 1) embedded in epoxy resin and sectioned transversely into discs were Raman-analyzed on coronal surfaces from <i>inter-</i> and <i>intra-tubular</i> dentine in 4 quadrants over 12, 24, and 48 min-acquisition times. Eight additional teeth stratified by root maturity, irrigated with NaOCl (<i>n</i> = 7) or saline (<i>n</i> = 1), sectioned transversely and then embedded, were Raman-analyzed on apical surfaces at 18 equidistant (50 μm) points/quadrant for Amide bands. Micro-indentation of the corresponding facing sectioned surface was correlated with Amide band changes. Generalized linear and non-linear regression models were used for data analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Spectral quality at 24/48 min was similar and better than at 12 min. Inter-tubular but not intra-tubular spectra were masked by fluorescence. Spectral features near the canal lumen ( < 500 μm) showed more significant collagen alteration and varied by tooth/quadrant but decreasingly towards the cemento-dentinal junction (CDJ) without a clear boundary. Significant (<i>p</i> < 0.0001) changes in Amide I/III bands up to 300 μm from the canal and were accompanied by deeper corresponding indentations upto 200 μm. Canal instrumentation had a significant (<i>p</i> < 0.0001) effect on both Amide-I and Amide-III bands.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>NaOCl altered dentinal collagen and reduced microhardness but varied with quadrants/teeth, without a definable boundary; collagen changes were obvious within 300 μm of the canal and microhardness changes within 200 μm but evident to a decreasing extent up to the CDJ.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Jaw Function and Mental Health Between Patients With Anterior Disc Displacement With and Without Reduction 前盘移位伴与不伴复位患者颌功能与心理健康的比较。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-15 DOI: 10.1002/cre2.70261

Yuan Yao, Sha Sha Liu, Lei Jin, Ji Ling Ye, Zhong Yi Fang, Yang Yang, Li Li Xu, Bin Cai, Xin Jiang

Objectives

Anterior disc displacement with reduction (ADDWR) and without reduction (ADDWOR) are two common clinical types of temporomandibular disorders. This study compared the levels of anxiety, depression, somatization, and jaw function of patients with ADDWR and ADDWOR.

Material and Methods

From January 2023 to December 2024, 119 patients with ADDWR and 127 patients with ADDWOR were recruited from the Department of Rehabilitation Medicine at the Shanghai Ninth Peoples Hospital. All participants completed a single assessment, including a pain rating and several questionnaires to assess jaw dysfunction, depression, somatization, and anxiety levels. The scores and the grade distribution of somatization, depression, and anxiety of the two groups were compared.

Results

Anxiety, depression, and somatization scores, as well as grade distributions, did not differ significantly between the ADDWR and ADDWOR groups. The scores for jaw function limitation, and pain on mouth opening and chewing were higher in the ADDWOR group than in the ADDWR group.

Conclusions

Patients with ADDWOR demonstrated more severely impaired jaw function and greater pain intensity during mouth opening and mastication compared to the ADDWR group. However, no significant differences were observed between the groups in terms of anxiety, depression, or somatic symptoms.

目的：前盘移位伴复位（ADDWR）和不复位（ADDWOR）是颞下颌关节疾病的两种常见临床类型。本研究比较了ADDWR和ADDWOR患者的焦虑、抑郁、躯体化和颌功能水平。材料与方法：2023年1月至2024年12月，从上海市第九人民医院康复医学科招募ADDWR患者119例，ADDWOR患者127例。所有的参与者都完成了一个单一的评估，包括疼痛评分和一些评估颚功能障碍、抑郁、躯体化和焦虑水平的问卷。比较两组患者躯体化、抑郁、焦虑的得分及等级分布。结果：焦虑、抑郁和躯体化得分以及等级分布在ADDWR组和ADDWOR组之间无显著差异。ADDWOR组颌骨功能受限、开口疼痛和咀嚼疼痛评分高于ADDWR组。结论：与ADDWR组相比，ADDWOR患者表现出更严重的颌功能受损，在张嘴和咀嚼时疼痛强度更大。然而，在焦虑、抑郁或躯体症状方面，两组之间没有观察到显著差异。

{"title":"Comparison of Jaw Function and Mental Health Between Patients With Anterior Disc Displacement With and Without Reduction","authors":"Yuan Yao, Sha Sha Liu, Lei Jin, Ji Ling Ye, Zhong Yi Fang, Yang Yang, Li Li Xu, Bin Cai, Xin Jiang","doi":"10.1002/cre2.70261","DOIUrl":"10.1002/cre2.70261","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Anterior disc displacement with reduction (ADDWR) and without reduction (ADDWOR) are two common clinical types of temporomandibular disorders. This study compared the levels of anxiety, depression, somatization, and jaw function of patients with ADDWR and ADDWOR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>From January 2023 to December 2024, 119 patients with ADDWR and 127 patients with ADDWOR were recruited from the Department of Rehabilitation Medicine at the Shanghai Ninth Peoples Hospital. All participants completed a single assessment, including a pain rating and several questionnaires to assess jaw dysfunction, depression, somatization, and anxiety levels. The scores and the grade distribution of somatization, depression, and anxiety of the two groups were compared.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Anxiety, depression, and somatization scores, as well as grade distributions, did not differ significantly between the ADDWR and ADDWOR groups. The scores for jaw function limitation, and pain on mouth opening and chewing were higher in the ADDWOR group than in the ADDWR group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Patients with ADDWOR demonstrated more severely impaired jaw function and greater pain intensity during mouth opening and mastication compared to the ADDWR group. However, no significant differences were observed between the groups in terms of anxiety, depression, or somatic symptoms.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporomandibular Disorders in 13- and 15-Year-Old Females: A Longitudinal Study 13岁和15岁女性颞下颌疾病：一项纵向研究。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-10 DOI: 10.1002/cre2.70257

Christina Mejersjö, Eva-Karin Bergström, Bengt Wenneberg, Anders Wänman

Objectives

Many adolescents, especially females, suffer from temporomandibular pain and dysfunction (TMD). We investigated the prevalence and progression of TMD symptoms in an early teen cohort in relation to oral parafunctions.

Methods

Girls 13 years of age in 19 middle schools were invited to participate. A total of 630 girls were enrolled in a prospective study at age 13 and 507 girls were followed at the age of 15 years. The girls completed a short questionnaire at the start and at follow-up. Clinical examination was performed at the start in 24% of the girls. All girls had regular check-ups at the Community Dentistry Clinic.

Results

Approximately one fourth of the 13-year-old girls reported TMD symptoms and headache once a week or more often. The frequencies increased during the follow-up to one third of the girls at age 15 years. Headache was the most common complaint with daily headache occurring in 7.5% of the girls at 15 years. At the clinical examination at age 13, one or more TMD signs were noticed in 36% of the girls with pain-related diagnoses in 32% (predominantly of muscle origin) and joint clicking in 4.5% of the girls. The need for some attention/care was estimated at 15%. There was a strong relationship between TMD symptoms and oral parafunctions.

Conclusions

Approximately one third of 13- and 15-year-old girls reported TMD symptoms and had clinical signs. It is crucial to take heed to TMD symptoms in young teenagers.

目的：许多青少年，尤其是女性，患有颞下颌关节疼痛和功能障碍（TMD）。我们调查了青少年早期TMD症状的患病率和进展与口腔功能障碍的关系。方法：选取19所中学13岁女生进行调查。共有630名女孩在13岁时参加了一项前瞻性研究，507名女孩在15岁时接受了随访。女孩们在开始和随访时完成了一份简短的问卷调查。24%的女孩在开始时进行了临床检查。所有女孩都在社区牙科诊所进行定期检查。结果：大约四分之一的13岁女孩报告TMD症状和头痛每周一次或更频繁。在对三分之一15岁女孩的随访中，这种频率增加了。头痛是最常见的主诉，15岁时7.5%的女孩每天都有头痛。在13岁的临床检查中，36%的女孩有一个或多个TMD症状，32%的女孩有疼痛相关的诊断（主要是肌肉起源），4.5%的女孩有关节咔嗒声。需要一些关注/照顾的估计为15%。TMD的症状与口腔功能有很强的关系。结论：大约三分之一的13岁和15岁的女孩报告了TMD症状并有临床体征。注意青少年的TMD症状是至关重要的。

{"title":"Temporomandibular Disorders in 13- and 15-Year-Old Females: A Longitudinal Study","authors":"Christina Mejersjö, Eva-Karin Bergström, Bengt Wenneberg, Anders Wänman","doi":"10.1002/cre2.70257","DOIUrl":"10.1002/cre2.70257","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Many adolescents, especially females, suffer from temporomandibular pain and dysfunction (TMD). We investigated the prevalence and progression of TMD symptoms in an early teen cohort in relation to oral parafunctions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Girls 13 years of age in 19 middle schools were invited to participate. A total of 630 girls were enrolled in a prospective study at age 13 and 507 girls were followed at the age of 15 years. The girls completed a short questionnaire at the start and at follow-up. Clinical examination was performed at the start in 24% of the girls. All girls had regular check-ups at the Community Dentistry Clinic.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Approximately one fourth of the 13-year-old girls reported TMD symptoms and headache once a week or more often. The frequencies increased during the follow-up to one third of the girls at age 15 years. Headache was the most common complaint with daily headache occurring in 7.5% of the girls at 15 years. At the clinical examination at age 13, one or more TMD signs were noticed in 36% of the girls with pain-related diagnoses in 32% (predominantly of muscle origin) and joint clicking in 4.5% of the girls. The need for some attention/care was estimated at 15%. There was a strong relationship between TMD symptoms and oral parafunctions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Approximately one third of 13- and 15-year-old girls reported TMD symptoms and had clinical signs. It is crucial to take heed to TMD symptoms in young teenagers.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145713536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue Biocompatibility and Antimicrobial Properties of Sympathomimetic Nasal Solutions for Potential Use in Dental Pulpal Management 拟交感神经鼻腔溶液在牙髓管理中潜在应用的组织生物相容性和抗菌性能。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-10 DOI: 10.1002/cre2.70259

Robert S. Jones, Roman Garcia, Isha Mutreja, Dhiraj Kumar

Objectives

Sympathomimetic nasal solutions (SNS), containing oxymetazoline or phenylephrine, may be used directly onto dental pulp tissue and initiate hemostasis via activation of alpha-receptors that innervate arteriole smooth muscles. The aim of this study was to assess these SNS's biocompatibility with human dental pulp stem cells (hDPSCs) and ability to kill a deep caries-associated pathogen, Rothia dentocariosa.

Materials and Methods

A Kirby-Bauer disk diffusion susceptibility test assessed SNS zones of inhibition (ZOI) toward R. dentocariosa growth on agar plates. Over-the-counter (OTC) SNS containing 0.025% and 0.05% oxymetazoline (NS-OXY-kids, NS-OXY-original) and 1% phenylephrine (NS-PHE) were tested along with ingredients found in these nasal solutions: 0.025%, 0.05% oxymetazoline; 0.0125%, 0.025% benzalkonium chloride; and 0.2%, 1% phenylephrine (PHE). A second antimicrobial broth inhibition assay examined effects on R. dentocariosa planktonic growth. A 24-h recovery biocompatibility assay with resazurin (alamarBlue) was performed on hDPSC that were exposed to pure respective compound solutions, NS-OXY-kids, NS-PHE, and 1% PHE for 10 min.

Results

NS-OXY-kids, NS-OXY-original, NS-PHE, and 0.025% BKC were bactericidal toward R. dentocariosa with an average ZOI of (16.33 ± 2.22 [average ± SD] mm). NS-PHE had a small but statistically significant difference (17.83 ± 3.189 mm) between NS-OXY-kids (14.67 ± 1.033 mm); 0.0125% BKC had a smaller (p < 0.05) ZOI of 10.5 ± 0.837 mm. Solutions containing solely OXY (0.025%, 0.05%) and PHE (0.2%, 1%) demonstrated no bactericidal activity but had a slight bacteriostatic inhibition in the broth inhibition assay. The commercial OTC formulations of NS-PHE and NS-OXY-kids needed to be diluted 1:10 to have near 50% metabolic activity of hDPSCs normalized to untreated control cells.

Conclusions

NS-PHE and NS-OXY (both original and kids) had antimicrobial activity toward R. dentocariosa and comparable biocompatibility with hDPSCs. SNS may have both hemostatic and antimicrobial properties if used as pulpal medicaments.

目的：含氧美唑啉或苯肾上腺素的拟交感鼻溶液（SNS）可直接应用于牙髓组织，通过激活支配小动脉平滑肌的α受体来启动止血。本研究的目的是评估这些SNS与人牙髓干细胞（hDPSCs）的生物相容性，以及杀死深部龋相关病原体牙齿罗氏菌（rosia dentocariosa）的能力。材料与方法：采用Kirby-Bauer圆盘扩散敏感性试验评价SNS在琼脂平板上对齿状核裂菌生长的抑制区（ZOI）。非处方（OTC） SNS含有0.025%和0.05% oxymetazoline （NS-OXY-kids, NS-OXY-original）和1% phenylephrine (NS-PHE)，以及在这些鼻溶液中发现的成分：0.025%,0.05% oxymetazoline；0.0125%， 0.025%苯扎氯铵；0.2%， 1%苯肾上腺素（PHE）。第二项抑菌肉汤抑制试验考察了对牙齿藻浮游生长的影响。将hDPSC分别暴露于NS-OXY-kids、NS-PHE和1% PHE的纯复合溶液中10分钟，用resazurin （alamarBlue）对hDPSC进行24小时恢复生物相容性测定。结果：NS-OXY-kids、NS-OXY-original、NS-PHE和0.025% BKC对齿状轮虫均有杀菌作用，平均ZOI为（16.33±2.22[平均±SD] mm）。NS-PHE与ns - oxy患儿（14.67±1.033 mm）差异虽小但有统计学意义（17.83±3.189 mm）；结论：NS-PHE和NS-OXY（无论是原始的还是儿童的）对齿状核裂菌都具有抗菌活性，并且与hdpsc具有相当的生物相容性。如果用作牙髓药物，SNS可能同时具有止血和抗菌特性。

{"title":"Tissue Biocompatibility and Antimicrobial Properties of Sympathomimetic Nasal Solutions for Potential Use in Dental Pulpal Management","authors":"Robert S. Jones, Roman Garcia, Isha Mutreja, Dhiraj Kumar","doi":"10.1002/cre2.70259","DOIUrl":"10.1002/cre2.70259","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Sympathomimetic nasal solutions (SNS), containing oxymetazoline or phenylephrine, may be used directly onto dental pulp tissue and initiate hemostasis via activation of alpha-receptors that innervate arteriole smooth muscles. The aim of this study was to assess these SNS's biocompatibility with human dental pulp stem cells (hDPSCs) and ability to kill a deep caries-associated pathogen, <i>Rothia dentocariosa</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A Kirby-Bauer disk diffusion susceptibility test assessed SNS zones of inhibition (ZOI) toward <i>R. dentocariosa</i> growth on agar plates. Over-the-counter (OTC) SNS containing 0.025% and 0.05% oxymetazoline (NS-OXY-kids, NS-OXY-original) and 1% phenylephrine (NS-PHE) were tested along with ingredients found in these nasal solutions: 0.025%, 0.05% oxymetazoline; 0.0125%, 0.025% benzalkonium chloride; and 0.2%, 1% phenylephrine (PHE). A second antimicrobial broth inhibition assay examined effects on <i>R. dentocariosa</i> planktonic growth. A 24-h recovery biocompatibility assay with resazurin (alamarBlue) was performed on hDPSC that were exposed to pure respective compound solutions, NS-OXY-kids, NS-PHE, and 1% PHE for 10 min.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>NS-OXY-kids, NS-OXY-original, NS-PHE, and 0.025% BKC were bactericidal toward <i>R. dentocariosa</i> with an average ZOI of (16.33 ± 2.22 [average ± SD] mm). NS-PHE had a small but statistically significant difference (17.83 ± 3.189 mm) between NS-OXY-kids (14.67 ± 1.033 mm); 0.0125% BKC had a smaller (<i>p</i> < 0.05) ZOI of 10.5 ± 0.837 mm. Solutions containing solely OXY (0.025%, 0.05%) and PHE (0.2%, 1%) demonstrated no bactericidal activity but had a slight bacteriostatic inhibition in the broth inhibition assay. The commercial OTC formulations of NS-PHE and NS-OXY-kids needed to be diluted 1:10 to have near 50% metabolic activity of hDPSCs normalized to untreated control cells.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>NS-PHE and NS-OXY (both original and kids) had antimicrobial activity toward <i>R. dentocariosa</i> and comparable biocompatibility with hDPSCs. SNS may have both hemostatic and antimicrobial properties if used as pulpal medicaments.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70259","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145713542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Outcomes of Titanium Mesh for Alveolar Bone Augmentation: An Umbrella Review 钛网用于牙槽骨增强的临床效果：综述。

IF 2.2 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research

Pub Date : 2025-12-10 DOI: 10.1002/cre2.70250

Ethan Ng, Nikos Donos, Mohammad Adib Jaafar, John Rong Hao Tay, Nikos Mardas

Objectives

To critically evaluate the evidence derived from systematic reviews for titanium mesh as a bone augmentation method for alveolar ridge deficiencies.

Materials and Methods

Five databases were searched for systematic reviews describing any interventions to increase the dimensions of the alveolar ridge using titanium mesh. Included reviews had to report on the primary outcome of vertical and/or horizontal bone gain after augmentation with titanium mesh. The methodological quality of the systematic reviews was assessed with the AMSTAR 2 tool, and the certainty of evidence was evaluated with GRADE.

Results

Eight systematic reviews were identified, including two with meta-analysis. Of the 51 index studies included in the reviews, 20 were RCTs. The weighted mean reported vertical bone gain was 4.05 mm, and horizontal gain was 3.96 mm. However, overlap of primary studies across included reviews limits the precision of these estimates. When stratified by review quality, the single high-quality review showed lower vertical and horizontal bone gain compared to the weighted mean. The average mesh exposure rate was 25%, although bone regeneration was usually still sufficient for implant placement.

Conclusions

Titanium mesh can be effective for bone regeneration but carries a notable risk of site exposure. When choosing the technique for bone augmentation, clinicians should balance achieving optimal bone regeneration with minimal complications to enhance patient care and surgical outcomes.

目的：对钛网作为牙槽嵴缺损骨增强方法的系统评价得出的证据进行批判性评价。材料和方法：我们检索了5个数据库，以系统回顾描述任何使用钛网增加牙槽嵴尺寸的干预措施。纳入的综述必须报告钛网增强后垂直和/或水平骨增加的主要结果。用AMSTAR 2工具评估系统评价的方法学质量，用GRADE评估证据的确定性。结果：确定了8个系统评价，包括2个荟萃分析。在纳入综述的51项指标研究中，有20项为随机对照试验。垂直骨增重的加权平均值为4.05 mm，水平骨增重为3.96 mm。然而，在纳入的综述中，主要研究的重叠限制了这些估计的准确性。当按评价质量分层时，单一高质量评价显示，与加权平均值相比，垂直和水平骨增重较低。平均网片暴露率为25%，尽管骨再生通常仍足以用于种植体放置。结论：钛网可有效修复骨缺损，但有暴露部位的危险。在选择骨增强技术时，临床医生应平衡实现最佳骨再生和最小的并发症，以提高患者护理和手术效果。

{"title":"Clinical Outcomes of Titanium Mesh for Alveolar Bone Augmentation: An Umbrella Review","authors":"Ethan Ng, Nikos Donos, Mohammad Adib Jaafar, John Rong Hao Tay, Nikos Mardas","doi":"10.1002/cre2.70250","DOIUrl":"10.1002/cre2.70250","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To critically evaluate the evidence derived from systematic reviews for titanium mesh as a bone augmentation method for alveolar ridge deficiencies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Five databases were searched for systematic reviews describing any interventions to increase the dimensions of the alveolar ridge using titanium mesh. Included reviews had to report on the primary outcome of vertical and/or horizontal bone gain after augmentation with titanium mesh. The methodological quality of the systematic reviews was assessed with the AMSTAR 2 tool, and the certainty of evidence was evaluated with GRADE.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Eight systematic reviews were identified, including two with meta-analysis. Of the 51 index studies included in the reviews, 20 were RCTs. The weighted mean reported vertical bone gain was 4.05 mm, and horizontal gain was 3.96 mm. However, overlap of primary studies across included reviews limits the precision of these estimates. When stratified by review quality, the single high-quality review showed lower vertical and horizontal bone gain compared to the weighted mean. The average mesh exposure rate was 25%, although bone regeneration was usually still sufficient for implant placement.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Titanium mesh can be effective for bone regeneration but carries a notable risk of site exposure. When choosing the technique for bone augmentation, clinicians should balance achieving optimal bone regeneration with minimal complications to enhance patient care and surgical outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12690612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145713539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0