This study used three-dimensional (3D) finite-element analysis (FEA) to investigate the effect of different implant placement strategies on the biomechanical behavior of implant-supported maxillary overdentures, and provide an initial guide to clinical treatment.
Materials and Methods
For an edentulous maxilla, six different implant-supported overdenture models with various implant placement strategies were created using CATIA software. The reference model (5R-3R-3L-5L) featured symmetrical implant placement in the canine and second premolar regions bilaterally. Five additional models incorporated asymmetrical implant placement strategies: 5R-3R-1L-3L, 5R-2R-3L-5L, 5R-4R-3L-5L, 4R-3R-3L-5L, and 6R-3R-3L-5L. All models had identical bone properties, prosthetic components, material characteristics, and loading conditions. The geometric models were analyzed using ANSYS 24.0 Workbench software. The maximum principal stress for bone, and stress distribution patterns were analyzed, and the performance of the models was compared with the symmetrical reference model.
Results
The quantitative and qualitative results showed that the implant placement strategy significantly influenced the magnitude and distribution of stress. The symmetrical implant placement strategy demonstrated the most favorable stress distribution, with the lowest maximum stress values in positions 5 R (2.69 MPa), 3 R (2.25 MPa), 3 L (2.16 MPa), and 5 L (3.24 MPa). Placement of implants in the anterior region resulted in stress concentration in the anterior region with maximum stress values at positions 5 R (3.24 MPa), 3 L (3.96 MPa), 1 L (5.09 MPa), and 3 L (5.57 MPa). Asymmetrical implant placement strategies with increased anteroposterior distribution and more posterior placement also demonstrated favorable biomechanical performance. Certain asymmetrical patterns induced fulcrum effects, leading to heterogeneous stress distribution.
Conclusions
The symmetrical (5R-3R-3L-5L) implant placement may provide a more uniform stress distribution, which may enhance peri-implant bone preservation and long-term implant stability. Implant placement in the canine region should be prioritized, while mesially-positioned implants warrant clinical caution due to higher stress levels in bilaterally symmetrical implant placement strategies.
目的：采用三维有限元分析（3D）研究不同种植策略对种植支撑上颌覆盖义齿生物力学行为的影响，为临床治疗提供初步指导。材料与方法：针对无牙上颌，采用CATIA软件制作6种不同种植体放置策略的种植支撑覆盖义齿模型。参考模型（5R-3R-3L-5L）在犬齿和第二前磨牙区域两侧对称放置种植体。另外五个模型采用不对称种植策略：5R-3R-1L-3L， 5R-2R-3L-5L, 5R-4R-3L-5L， 4R-3R-3L-5L和6R-3R-3L-5L。所有模型具有相同的骨特性、假体部件、材料特性和加载条件。采用ANSYS 24.0 Workbench软件对几何模型进行分析。分析了骨的最大主应力和应力分布规律，并与对称参考模型的性能进行了比较。结果：定量和定性结果表明，种植体放置策略对应力的大小和分布有显著影响。对称种植体的应力分布最有利，最大应力值在5 R （2.69 MPa）、3 R （2.25 MPa）、3 L （2.16 MPa）和5 L （3.24 MPa）位置最小。在前牙区放置种植体导致前牙区应力集中，最大应力值在5 R （3.24 MPa）、3 L （3.96 MPa）、1 L （5.09 MPa）和3 L （5.57 MPa）位置。增加前后位分布和更多后位的不对称种植体放置策略也显示出良好的生物力学性能。某些不对称图案引起支点效应，导致应力分布不均匀。结论：对称（5R-3R-3L-5L）种植体放置可以提供更均匀的应力分布，可以增强种植体周围骨保存和长期种植体稳定性。种植体放置在犬区应优先考虑，而中间位置的种植体需要临床谨慎，因为在双侧对称种植体放置策略中，压力水平较高。
{"title":"Influence of Various Implant Placement Strategies on Stress Distribution in Maxillary Implant-Retained Overdenture: A 3D Finite-Element Analysis","authors":"Zahra Ghorbani, Ebrahim Shojaei, Hamid Neshandar Asli, Mehran Falahchai","doi":"10.1002/cre2.70283","DOIUrl":"10.1002/cre2.70283","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study used three-dimensional (3D) finite-element analysis (FEA) to investigate the effect of different implant placement strategies on the biomechanical behavior of implant-supported maxillary overdentures, and provide an initial guide to clinical treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>For an edentulous maxilla, six different implant-supported overdenture models with various implant placement strategies were created using CATIA software. The reference model (5R-3R-3L-5L) featured symmetrical implant placement in the canine and second premolar regions bilaterally. Five additional models incorporated asymmetrical implant placement strategies: 5R-3R-1L-3L, 5R-2R-3L-5L, 5R-4R-3L-5L, 4R-3R-3L-5L, and 6R-3R-3L-5L. All models had identical bone properties, prosthetic components, material characteristics, and loading conditions. The geometric models were analyzed using ANSYS 24.0 Workbench software. The maximum principal stress for bone, and stress distribution patterns were analyzed, and the performance of the models was compared with the symmetrical reference model.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The quantitative and qualitative results showed that the implant placement strategy significantly influenced the magnitude and distribution of stress. The symmetrical implant placement strategy demonstrated the most favorable stress distribution, with the lowest maximum stress values in positions 5 R (2.69 MPa), 3 R (2.25 MPa), 3 L (2.16 MPa), and 5 L (3.24 MPa). Placement of implants in the anterior region resulted in stress concentration in the anterior region with maximum stress values at positions 5 R (3.24 MPa), 3 L (3.96 MPa), 1 L (5.09 MPa), and 3 L (5.57 MPa). Asymmetrical implant placement strategies with increased anteroposterior distribution and more posterior placement also demonstrated favorable biomechanical performance. Certain asymmetrical patterns induced fulcrum effects, leading to heterogeneous stress distribution.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The symmetrical (5R-3R-3L-5L) implant placement may provide a more uniform stress distribution, which may enhance peri-implant bone preservation and long-term implant stability. Implant placement in the canine region should be prioritized, while mesially-positioned implants warrant clinical caution due to higher stress levels in bilaterally symmetrical implant placement strategies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70283","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145932259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José A Falcón-Flores, María E Jiménez-Corona, Ileana G Rangel-Nieto, Marisela Vazquez-Duran, Aida Jiménez-Corona
Objective
We assessed the reliability and validity of a self-report instrument to ascertain periodontitis risk.
Materials and Methods
A cross-sectional population-based study was conducted in 2013 involving 454 adult people. The instrument included items on periodontal status such as self-perception of gum health, bleeding and gingival infection, halitosis, tooth mobility, and tooth loss. The periodontal clinical condition was assessed using the Periodontal Screening and Recording Index. Construct validity was evaluated by exploratory factor analysis (EFA). Sensitivity, specificity, and area under the receiver operating characteristic curve (AUC-ROC) were calculated as well. Reliability was assessed using Cronbach's alpha.
Results
The prevalence of clinically evaluated moderate (MP) and severe (SP) periodontitis was 49.3% and 28.9%, respectively. Two factors for both types of periodontitis were identified using EFA. The combination of self-report items and risk factors for periodontitis showed an AUC-ROC of 0.660 (sensitivity 97.3%, specificity 3%) for MP and 0.804 (sensitivity 96.9%, specificity 28.3%) for SP.
Conclusions
EFA showed two factors that accounted for the baseline and outcome stage of periodontitis and better predicted the risk of SP. This instrument can be an alternative for monitoring this disease at the population level.
{"title":"Exploratory Evaluation of Self-Reported Periodontitis Among Adult Population From Comitán Chiapas, Mexico","authors":"José A Falcón-Flores, María E Jiménez-Corona, Ileana G Rangel-Nieto, Marisela Vazquez-Duran, Aida Jiménez-Corona","doi":"10.1002/cre2.70274","DOIUrl":"10.1002/cre2.70274","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>We assessed the reliability and validity of a self-report instrument to ascertain periodontitis risk.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A cross-sectional population-based study was conducted in 2013 involving 454 adult people. The instrument included items on periodontal status such as self-perception of gum health, bleeding and gingival infection, halitosis, tooth mobility, and tooth loss. The periodontal clinical condition was assessed using the Periodontal Screening and Recording Index. Construct validity was evaluated by exploratory factor analysis (EFA). Sensitivity, specificity, and area under the receiver operating characteristic curve (AUC-ROC) were calculated as well. Reliability was assessed using Cronbach's alpha.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The prevalence of clinically evaluated moderate (MP) and severe (SP) periodontitis was 49.3% and 28.9%, respectively. Two factors for both types of periodontitis were identified using EFA. The combination of self-report items and risk factors for periodontitis showed an AUC-ROC of 0.660 (sensitivity 97.3%, specificity 3%) for MP and 0.804 (sensitivity 96.9%, specificity 28.3%) for SP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>EFA showed two factors that accounted for the baseline and outcome stage of periodontitis and better predicted the risk of SP. This instrument can be an alternative for monitoring this disease at the population level.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12789814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145942650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kim N. Stolte, Anna Fedorova, Sameh Attia, Kerstin Danker, Henrik Dommisch
Objectives
The Janus kinase (JAK)/Signal Transducer and Activator of Transcription (STAT) pathway plays a crucial role in oral inflammatory diseases such as oral lichen planus (OLP) and periodontitis. Interferon-gamma (IFN-γ) is a cytokine that activates the JAK/STAT pathway. This study aimed to evaluate the effect of the JAK1/2 inhibitor ruxolitinib on IFN-γ-driven inflammation in oral keratinocytes.
Material and Methods
Human oral keratinocytes (OKG4) were stimulated with 10 ng/mL IFN-γ to assess the expression and phosphorylation status of JAK1, JAK2, STAT1, STAT2, and human leukocyte antigen (HLA)-DRB1. Ruxolitinib (1 µM) was used to inhibit IFN-γ-induced signaling. Analyses were performed using Western blotting, immunofluorescence microscopy, and cell viability assays.
Results
IFN-γ significantly upregulated pSTAT1 at 30 min (control: 1.00 ± 0.00, IFN-γ: 29.17 ± 9.26, p = 0.0013), with sustained activation after 24 h (IFN-γ: 29.49 ± 18.26, p = 0.0127). HLA-DRB1 expression was also increased (control: 1.00 ± 0.00, IFN-γ: 3.51 ± 1.28, p = 0.0127). Co-treatment with IFN-γ and ruxolitinib reduced both pSTAT1 (p = 0.3448) and HLA-DRB1 (p = 0.3448) expression to baseline levels. Ruxolitinib had no effect on cell viability (p > 0.9999).
Conclusions
IFN-γ induces robust and sustained activation of STAT1 and upregulation of HLA-DRB1 in oral keratinocytes, both effectively suppressed by ruxolitinib. These findings underscore the therapeutic potential of targeting the JAK/STAT pathway in oral inflammatory diseases.
{"title":"Ruxolitinib Suppresses Interferon-γ-Induced JAK/STAT Activation in Oral Keratinocytes","authors":"Kim N. Stolte, Anna Fedorova, Sameh Attia, Kerstin Danker, Henrik Dommisch","doi":"10.1002/cre2.70281","DOIUrl":"10.1002/cre2.70281","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The Janus kinase (JAK)/Signal Transducer and Activator of Transcription (STAT) pathway plays a crucial role in oral inflammatory diseases such as oral lichen planus (OLP) and periodontitis. Interferon-gamma (IFN-γ) is a cytokine that activates the JAK/STAT pathway. This study aimed to evaluate the effect of the JAK1/2 inhibitor ruxolitinib on IFN-γ-driven inflammation in oral keratinocytes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Human oral keratinocytes (OKG4) were stimulated with 10 ng/mL IFN-γ to assess the expression and phosphorylation status of JAK1, JAK2, STAT1, STAT2, and human leukocyte antigen (HLA)-DRB1. Ruxolitinib (1 µM) was used to inhibit IFN-γ-induced signaling. Analyses were performed using Western blotting, immunofluorescence microscopy, and cell viability assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>IFN-γ significantly upregulated pSTAT1 at 30 min (control: 1.00 ± 0.00, IFN-γ: 29.17 ± 9.26, <i>p</i> = 0.0013), with sustained activation after 24 h (IFN-γ: 29.49 ± 18.26, <i>p</i> = 0.0127). HLA-DRB1 expression was also increased (control: 1.00 ± 0.00, IFN-γ: 3.51 ± 1.28, <i>p</i> = 0.0127). Co-treatment with IFN-γ and ruxolitinib reduced both pSTAT1 (<i>p</i> = 0.3448) and HLA-DRB1 (<i>p</i> = 0.3448) expression to baseline levels. Ruxolitinib had no effect on cell viability (<i>p</i> > 0.9999).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>IFN-γ induces robust and sustained activation of STAT1 and upregulation of HLA-DRB1 in oral keratinocytes, both effectively suppressed by ruxolitinib. These findings underscore the therapeutic potential of targeting the JAK/STAT pathway in oral inflammatory diseases.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145942721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This in vitro investigation evaluated the microshear bond strength (µSBS) of composite resin to mineral trioxide aggregate (MTA) and a modified polycarboxylate cement containing potassium nitrate (PCC/KNO3), both utilized as pulpotomy materials. The performance of two universal adhesives was compared, applied using self-etch (SE) and etch-and-rinse (E&R) strategies.
Materials and Methods
A total of 192 cylindrical acrylic specimens (2 cm height × 1 cm diameter) with a central cavity (4 mm diameter × 2 mm depth) were fabricated and filled with either MTA or PCC/KNO3 (n = 96 each). These were further divided into eight experimental subgroups (n = 12) based on adhesive type (All-Bond Universal or Gluma Bond Universal), adhesive application method (SE or E&R), and storage duration (24 h or 7 days). After resin composite application, all specimens were stored in 100% humidity at 37°C for 24 h prior to µSBS testing. Data were analyzed using four-way ANOVA and independent t-tests (α = 0.05).
Results
PCC/KNO3 exhibited significantly greater bond strength than MTA at both 24-h (p < 0.001) and 7-day (p = 0.030) intervals. Neither the adhesive type (p = 0.355) nor the application method (p = 0.358) significantly affected bond strength. Over time, µSBS values increased for MTA in some groups, while a significant decline was observed in most PCC/KNO3 subgroups.
Conclusion
Due to its superior early bond strength and compatibility with immediate restoration, PCC/KNO3 shows potential as a viable alternative to MTA in vital pulp therapy, regardless of the adhesive strategy employed.
目的：本研究评估了复合树脂与矿物三氧化物骨料（MTA）和含硝酸钾改性聚羧酸盐水泥（PCC/KNO3）的微剪切结合强度（µSBS），这两种材料均可作为切髓材料。比较了两种通用胶粘剂的性能，采用自蚀刻（SE）和蚀刻-漂洗（E&R）策略。材料和方法：制作192个圆柱形丙烯酸样品（高2 cm ×直径1 cm），中心腔（直径4 mm × 2 mm深），填充MTA或PCC/KNO3 （n = 96）。根据粘接类型（All-Bond Universal或Gluma Bond Universal）、粘接方式（SE或E&R）和保存时间（24 h或7 d）将其分为8个实验亚组（n = 12）。树脂复合材料应用后，所有样品在37°C的100%湿度下保存24 h，然后进行µSBS测试。数据分析采用四因素方差分析和独立t检验（α = 0.05）。结果：PCC/KNO3在两个24小时亚组的结合强度均显著高于MTA。结论：由于PCC/KNO3具有优越的早期粘结强度和与即时修复的相容性，无论采用何种粘结策略，PCC/KNO3都有可能成为MTA在重要牙髓治疗中的可行替代方案。
{"title":"Microshear Bond Strength of Composite Resin to Mineral Trioxide Aggregate and Potassium Nitrate-Modified Polycarboxylate Cement at Two Time Points Using Different Adhesive Approaches: An In Vitro Study","authors":"Alireza Adl, Zahra Jowkar, Mahdi Zerafat, Fereshte Sobhnamayan","doi":"10.1002/cre2.70287","DOIUrl":"10.1002/cre2.70287","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This in vitro investigation evaluated the microshear bond strength (µSBS) of composite resin to mineral trioxide aggregate (MTA) and a modified polycarboxylate cement containing potassium nitrate (PCC/KNO<sub>3</sub>), both utilized as pulpotomy materials. The performance of two universal adhesives was compared, applied using self-etch (SE) and etch-and-rinse (E&R) strategies.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A total of 192 cylindrical acrylic specimens (2 cm height × 1 cm diameter) with a central cavity (4 mm diameter × 2 mm depth) were fabricated and filled with either MTA or PCC/KNO<sub>3</sub> (<i>n</i> = 96 each). These were further divided into eight experimental subgroups (<i>n</i> = 12) based on adhesive type (All-Bond Universal or Gluma Bond Universal), adhesive application method (SE or E&R), and storage duration (24 h or 7 days). After resin composite application, all specimens were stored in 100% humidity at 37°C for 24 h prior to µSBS testing. Data were analyzed using four-way ANOVA and independent <i>t</i>-tests (<i>α</i> = 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>PCC/KNO<sub>3</sub> exhibited significantly greater bond strength than MTA at both 24-h (<i>p</i> < 0.001) and 7-day (<i>p</i> = 0.030) intervals. Neither the adhesive type (<i>p</i> = 0.355) nor the application method (<i>p</i> = 0.358) significantly affected bond strength. Over time, µSBS values increased for MTA in some groups, while a significant decline was observed in most PCC/KNO<sub>3</sub> subgroups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Due to its superior early bond strength and compatibility with immediate restoration, PCC/KNO<sub>3</sub> shows potential as a viable alternative to MTA in vital pulp therapy, regardless of the adhesive strategy employed.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145932291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathan E. Estrin, Troy B. Tran, Alan Rene Espinoza, Paras Ahmad, Nima Farshidfar, Ryan Holmes, Yufeng Zhang, Richard J. Miron
<div> <section> <h3> Introduction</h3> <p>Platelet-rich fibrin (PRF) has been commonly utilized for ridge preservation techniques either to introduce supraphysiological concentrations of autologous growth factors to the defect area (typically when mixed within a bone graft) or utilized alone as a solo “barrier” membrane. Noteworthy, however, one of the commonly reported drawbacks of PRF is its relatively short resorption period characterized by lasting roughly 2 weeks. This may therefore be insufficient for complete soft tissue closure and/or preventing soft tissue cells from infiltrating into the bony compartment. Recently, it was discovered that by heating plasma and denaturing albumin using the Bio-Heat technology, the resorption properties of PRF could be extended from a standard 2–3 week period toward 4–6 months. The aim of the present human case series was to investigate for the first time the safety and applicability of utilizing this novel 100% autologous extended-PRF (e-PRF) membrane for ridge preservation.</p> </section> <section> <h3> Materials and Methods</h3> <p>Twenty-two patients requiring 22 single tooth posterior extractions were included in this case series. In all cases, atraumatic extractions were performed, and the sites were grafted using a combination of bone allograft and standard PRF to create “sticky bone.” Noteworthy, the barrier membrane utilized over top of the bone graft was the novel e-PRF, which was utilized as a solo membrane in place of standard collagen or polytetrafluoroethylene (PTFE) membranes. Cone-beam computed tomography scans were taken immediately after extractions and at 3 months postoperatively. Ridge width at 1, 3, and 5 mm apical to the crest, and buccal and lingual height dimensions were recorded at both time intervals. Additionally, buccal bone thickness at 1, 3, and 5 mm apical to the crest was recorded at baseline.</p> </section> <section> <h3> Results</h3> <p>All extraction sites healed uneventfully without any postoperative complications. No clinical signs of infection or other complications were detected. The mean change in ridge width at 1, 3, and 5 mm apical to the crest was −1.27 ± 0.70, −0.94 ± 0.80, and −0.69 ± 0.79 mm, respectively. The mean change in buccal height and lingual height was −1.25 ± 1.16 and −0.94 ± 1.07 mm, respectively.</p> </section> <section> <h3> Conclusions</h3> <p>The use of e-PRF membranes in place of collagen membranes for ridge preservation was shown to be an effective, safe, and predictable treatment modality. The e-PRF membranes can be fabricated at low cost with a
{"title":"Safety and Feasibility of Extended Platelet-Rich Fibrin as a Solo Barrier Membrane for Ridge Preservation: A Case Series","authors":"Nathan E. Estrin, Troy B. Tran, Alan Rene Espinoza, Paras Ahmad, Nima Farshidfar, Ryan Holmes, Yufeng Zhang, Richard J. Miron","doi":"10.1002/cre2.70282","DOIUrl":"10.1002/cre2.70282","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Platelet-rich fibrin (PRF) has been commonly utilized for ridge preservation techniques either to introduce supraphysiological concentrations of autologous growth factors to the defect area (typically when mixed within a bone graft) or utilized alone as a solo “barrier” membrane. Noteworthy, however, one of the commonly reported drawbacks of PRF is its relatively short resorption period characterized by lasting roughly 2 weeks. This may therefore be insufficient for complete soft tissue closure and/or preventing soft tissue cells from infiltrating into the bony compartment. Recently, it was discovered that by heating plasma and denaturing albumin using the Bio-Heat technology, the resorption properties of PRF could be extended from a standard 2–3 week period toward 4–6 months. The aim of the present human case series was to investigate for the first time the safety and applicability of utilizing this novel 100% autologous extended-PRF (e-PRF) membrane for ridge preservation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Twenty-two patients requiring 22 single tooth posterior extractions were included in this case series. In all cases, atraumatic extractions were performed, and the sites were grafted using a combination of bone allograft and standard PRF to create “sticky bone.” Noteworthy, the barrier membrane utilized over top of the bone graft was the novel e-PRF, which was utilized as a solo membrane in place of standard collagen or polytetrafluoroethylene (PTFE) membranes. Cone-beam computed tomography scans were taken immediately after extractions and at 3 months postoperatively. Ridge width at 1, 3, and 5 mm apical to the crest, and buccal and lingual height dimensions were recorded at both time intervals. Additionally, buccal bone thickness at 1, 3, and 5 mm apical to the crest was recorded at baseline.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All extraction sites healed uneventfully without any postoperative complications. No clinical signs of infection or other complications were detected. The mean change in ridge width at 1, 3, and 5 mm apical to the crest was −1.27 ± 0.70, −0.94 ± 0.80, and −0.69 ± 0.79 mm, respectively. The mean change in buccal height and lingual height was −1.25 ± 1.16 and −0.94 ± 1.07 mm, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The use of e-PRF membranes in place of collagen membranes for ridge preservation was shown to be an effective, safe, and predictable treatment modality. The e-PRF membranes can be fabricated at low cost with a","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70282","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145932408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giulia Brunello, Direm Ilter, Florian Fürst, Jürgen Becker, Charlotte von Gall, Kathrin Becker, Beryl Schwarz-Herzke
Objectives
Acellular xenogeneic matrices are becoming increasingly popular for periodontal and peri-implant soft tissue augmentation and recession coverage. This study aimed to investigate the behavior of bone-derived stromal cells cultured onto three commercially available collagen membranes (Fibro-Gide®, mucoderm®, and NovoMatrix®).
Methods
Cells were isolated from alveolar bone chips of four adult healthy patients and were separately seeded on the membranes. Cells cultured in the absence of biomaterials were used as a control. Number and viability of cells on and around the membranes were evaluated at different timepoints. Actin cytoskeleton change was visualized with phalloidin staining. Markers for adhesion (VCAM-1, FAK, and fibronectin) were assessed by immunofluorescence.
Results
The number and viability of cells grown on the membranes were significantly lower than those of the controls at D7. Moreover, cells grown on and around all three membranes showed changes in actin cytoskeleton reminiscent of stress fibers. Cells grown around NovoMatrix® also show reduced viability. The three membranes had no effect on the adhesion markers of cells growing around them.
Conclusions
All three membranes resulted in a reduction in viability and increased cell stress of adherent cells compared to the control. A reduced viability was detected, in particular, in cells growing around NovoMatrix®. Morphologically, the cells showed signs of stress. This reaction indicates that the membranes may release substances that could impair the healing process in vivo, especially in the case of fast degradation.
{"title":"Proliferation, Adhesion, and Morphology of Bone-Derived Stromal Cells on Xenogenic Collagen Matrices: An In Vitro Study","authors":"Giulia Brunello, Direm Ilter, Florian Fürst, Jürgen Becker, Charlotte von Gall, Kathrin Becker, Beryl Schwarz-Herzke","doi":"10.1002/cre2.70288","DOIUrl":"10.1002/cre2.70288","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Acellular xenogeneic matrices are becoming increasingly popular for periodontal and peri-implant soft tissue augmentation and recession coverage. This study aimed to investigate the behavior of bone-derived stromal cells cultured onto three commercially available collagen membranes (Fibro-Gide®, mucoderm®, and NovoMatrix®).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Cells were isolated from alveolar bone chips of four adult healthy patients and were separately seeded on the membranes. Cells cultured in the absence of biomaterials were used as a control. Number and viability of cells on and around the membranes were evaluated at different timepoints. Actin cytoskeleton change was visualized with phalloidin staining. Markers for adhesion (VCAM-1, FAK, and fibronectin) were assessed by immunofluorescence.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The number and viability of cells grown on the membranes were significantly lower than those of the controls at D7. Moreover, cells grown on and around all three membranes showed changes in actin cytoskeleton reminiscent of stress fibers. Cells grown around NovoMatrix® also show reduced viability. The three membranes had no effect on the adhesion markers of cells growing around them.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>All three membranes resulted in a reduction in viability and increased cell stress of adherent cells compared to the control. A reduced viability was detected, in particular, in cells growing around NovoMatrix®. Morphologically, the cells showed signs of stress. This reaction indicates that the membranes may release substances that could impair the healing process in vivo, especially in the case of fast degradation.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"12 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145932330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iman Rajaei, Azadeh Babaei, Shirin Djalalinia, Mostafa Qorbani
Objectives
Children with autism spectrum disorder (ASD) exhibit a higher prevalence of oral health diseases. These oral health issues significantly impair quality of life and increase caregiver burden. Despite emerging interventions like specialized behavioral guidance and caregiver training, a comprehensive evaluation of their effectiveness remains absent. This systematic review and meta-analysis was designed to pool the effectiveness of Oral health interventions in children with ASD.
Materials and Methods
In this systematic review, some international databases, including PubMed, ISI/WOS, CENTRAL, and Scopus, were searched via appropriate keywords until January 1, 2025. All clinical trials that assessed the effect of interventions on oral health indices including Simplified Oral Hygiene Index (OHI-S), plaque index (PI), and gingival index (GI) without any language or time restrictions were included in the study. Search strategy process, screening, data extraction, and quality assessment were performed by two experts independently. Heterogeneity between studies was assessed using I2 and Q-Cochrane test. Random effect meta-analysis was performed to pool the standardized mean difference (SMD) with 95% confidence intervals (CIs).
Results
Overall, 27 studies with 1918 participants were included in this study. Interventions are categorized into the educational (visual, verbal, booklet/leaflet) and procedural approaches. Random effect meta-analysis showed significant improvements for PI (SMD = −0.73, 95% CI: [−1.02 to −0.44]) and OHI-S (SMD = −1.44, 95% CI: [−2.79 to −0.08]), with video interventions notably effective for PI (SMD = −0.69, 95% CI: [−1.2 to −0.14]). The GI index also improved (SMD = −0.74, 95% CI: [−1.34 to −0.14]).
Conclusions
Visual pedagogy, particularly videos, and parental involvement significantly improved oral health in children with ASD. Video-based interventions should be added to traditional methods such as verbal or picture-based approaches to improve oral health interventions in ASD.
{"title":"Oral Health Interventions in Children With Autism Spectrum Disorder: A Systematic Review and Meta-Analysis of Interventional Studies","authors":"Iman Rajaei, Azadeh Babaei, Shirin Djalalinia, Mostafa Qorbani","doi":"10.1002/cre2.70258","DOIUrl":"10.1002/cre2.70258","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Children with autism spectrum disorder (ASD) exhibit a higher prevalence of oral health diseases. These oral health issues significantly impair quality of life and increase caregiver burden. Despite emerging interventions like specialized behavioral guidance and caregiver training, a comprehensive evaluation of their effectiveness remains absent. This systematic review and meta-analysis was designed to pool the effectiveness of Oral health interventions in children with ASD.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>In this systematic review, some international databases, including PubMed, ISI/WOS, CENTRAL, and Scopus, were searched via appropriate keywords until January 1, 2025. All clinical trials that assessed the effect of interventions on oral health indices including Simplified Oral Hygiene Index (OHI-S), plaque index (PI), and gingival index (GI) without any language or time restrictions were included in the study. Search strategy process, screening, data extraction, and quality assessment were performed by two experts independently. Heterogeneity between studies was assessed using I<sup>2</sup> and Q-Cochrane test. Random effect meta-analysis was performed to pool the standardized mean difference (SMD) with 95% confidence intervals (CIs).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Overall, 27 studies with 1918 participants were included in this study. Interventions are categorized into the educational (visual, verbal, booklet/leaflet) and procedural approaches. Random effect meta-analysis showed significant improvements for PI (SMD = −0.73, 95% CI: [−1.02 to −0.44]) and OHI-S (SMD = −1.44, 95% CI: [−2.79 to −0.08]), with video interventions notably effective for PI (SMD = −0.69, 95% CI: [−1.2 to −0.14]). The GI index also improved (SMD = −0.74, 95% CI: [−1.34 to −0.14]).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Visual pedagogy, particularly videos, and parental involvement significantly improved oral health in children with ASD. Video-based interventions should be added to traditional methods such as verbal or picture-based approaches to improve oral health interventions in ASD.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Artificial Intelligence (AI) has emerged as a transformative tool in various scientific disciplines.
Objective
The study aims to provide a comprehensive bibliometric analysis of the literature on AI and root canal morphology, examining publication trends, author contributions, and thematic focus areas.
Methods
Data were retrieved from the Scopus database using the search string “Artificial Intelligence” OR “AI” AND “Root” OR “Root canal morphology,” covering publications from 1997 to 2024. Only articles and reviews were included. Vosviewer software was employed to visualize co-authorship networks and perform co-word analysis.
Results
The analysis encompassed 81 papers, 433 authors, and 274 departmental contributions across 31 countries. The documents were published in 52 journals. The analysis revealed a global collaboration network with significant contributions from diverse institutions and countries. By co-word analysis, the focus of the 81 papers is categorized into 25 distinct research areas. The thematic analysis identified key research areas and emerging trends within the literature. The findings highlight AI applications' increasing interest and interdisciplinary nature in root canal morphology research.
Conclusion
This study provides a detailed overview of the research landscape concerning AI and root canal morphology. It identified leading contributors and institutions and presented a structured view of research themes.
{"title":"AI in Root Canal Morphology: A Detailed Bibliometric Analysis of Research Trends and Global Contributions","authors":"Waseem Hassan, Vini Mehta, Niher Tabassum Singdha, Mohmed Isaqali Karobari","doi":"10.1002/cre2.70269","DOIUrl":"10.1002/cre2.70269","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Artificial Intelligence (AI) has emerged as a transformative tool in various scientific disciplines.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The study aims to provide a comprehensive bibliometric analysis of the literature on AI and root canal morphology, examining publication trends, author contributions, and thematic focus areas.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Data were retrieved from the Scopus database using the search string “Artificial Intelligence” OR “AI” AND “Root” OR “Root canal morphology,” covering publications from 1997 to 2024. Only articles and reviews were included. Vosviewer software was employed to visualize co-authorship networks and perform co-word analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis encompassed 81 papers, 433 authors, and 274 departmental contributions across 31 countries. The documents were published in 52 journals. The analysis revealed a global collaboration network with significant contributions from diverse institutions and countries. By co-word analysis, the focus of the 81 papers is categorized into 25 distinct research areas. The thematic analysis identified key research areas and emerging trends within the literature. The findings highlight AI applications' increasing interest and interdisciplinary nature in root canal morphology research.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study provides a detailed overview of the research landscape concerning AI and root canal morphology. It identified leading contributors and institutions and presented a structured view of research themes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shengjile Deari, Monika Gothwal, Kay Gränicher, Thomas Thurnheer, Thomas Attin, Lamprini Karygianni
Objectives
Oral biofilms initiate with the formation of an acquired pellicle on dental surfaces, a thin layer of salivary glycoproteins that provides a substrate for microbial adhesion. This study aimed to assess the necessity of a preformed pellicle for biofilm growth in vitro by analyzing the development of a standardized six-species biofilm, comprising Actinomyces oris, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, Streptococcus oralis, and Candida albicans.
Materials and Methods
Biofilms were cultivated on bovine enamel discs under two conditions: (1) precoated with human saliva to simulate a pellicle and (2) without a preformed pellicle. Colony-forming units (CFUs) of each microbial species were quantified after incubation in either human saliva or a NaCl-based medium at 16 and 64 h.
Results
The analysis revealed no significant differences in CFU counts between discs with or without a preformed pellicle, regardless of whether biofilms were grown in human saliva or NaCl medium, with one exception: S. oralis in pellicle/NaCl (6.7 Log10) medium at 16 h showed a slight decrease in the absence of a pellicle (5.9 Log10).
Conclusions
These findings suggest that microbial adhesion and subsequent biofilm development occurred independently of an initial pellicle. The preformed salivary pellicle does not seem to play a significant role in the initial development of this in vitro biofilm model. Biofilm testing in laboratory settings, especially for studies on antimicrobial efficacy, could be simplified, as pellicle formation may not be an essential requirement. Although no significant differences in biofilm development were observed between pellicle and no-pellicle conditions, the growth medium may have influenced pellicle interactions, warranting further investigation of media effects on pellicle formation. Existing assumptions about pellicle dependence in biofilm formation are challenged, and suggest that in vitro models without a pellicle may still provide valid platforms for studying biofilms and testing antimicrobial agents effectively.
{"title":"The Effect of Pellicle on Biofilm Formation in a Supragingival Biofilm Model","authors":"Shengjile Deari, Monika Gothwal, Kay Gränicher, Thomas Thurnheer, Thomas Attin, Lamprini Karygianni","doi":"10.1002/cre2.70276","DOIUrl":"10.1002/cre2.70276","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Oral biofilms initiate with the formation of an acquired pellicle on dental surfaces, a thin layer of salivary glycoproteins that provides a substrate for microbial adhesion. This study aimed to assess the necessity of a preformed pellicle for biofilm growth in vitro by analyzing the development of a standardized six-species biofilm, comprising <i>Actinomyces oris</i>, <i>Veillonella dispar</i>, <i>Fusobacterium nucleatum</i>, <i>Streptococcus sobrinus</i>, <i>Streptococcus oralis</i>, and <i>Candida albicans</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Biofilms were cultivated on bovine enamel discs under two conditions: (1) precoated with human saliva to simulate a pellicle and (2) without a preformed pellicle. Colony-forming units (CFUs) of each microbial species were quantified after incubation in either human saliva or a NaCl-based medium at 16 and 64 h.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The analysis revealed no significant differences in CFU counts between discs with or without a preformed pellicle, regardless of whether biofilms were grown in human saliva or NaCl medium, with one exception: S. oralis in pellicle/NaCl (6.7 Log<sub>10</sub>) medium at 16 h showed a slight decrease in the absence of a pellicle (5.9 Log<sub>10</sub>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings suggest that microbial adhesion and subsequent biofilm development occurred independently of an initial pellicle. The preformed salivary pellicle does not seem to play a significant role in the initial development of this in vitro biofilm model. Biofilm testing in laboratory settings, especially for studies on antimicrobial efficacy, could be simplified, as pellicle formation may not be an essential requirement. Although no significant differences in biofilm development were observed between pellicle and no-pellicle conditions, the growth medium may have influenced pellicle interactions, warranting further investigation of media effects on pellicle formation. Existing assumptions about pellicle dependence in biofilm formation are challenged, and suggest that in vitro models without a pellicle may still provide valid platforms for studying biofilms and testing antimicrobial agents effectively.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yan Chen, Chen‑xi Li, Rui Xue, Zhao-xia Cong, Yi Bian, Yuan Liu
Objectives
This study aimed to investigate the differentially expressed microRNAs in erosive oral lichen planus, followed by analyzing how the overexpression of identified miR-516a-5p influences human oral mucosal fibroblasts.
Material and Methods
High-throughput sequencing using tissues from patients and healthy individuals identified varying microRNA expression profiles in erosive oral lichen planus tissues. Bioinformatics analysis subsequently revealed the enriched pathways and targeted genes involved. In vitro experiments were performed to confirm the validity of bioinformatic findings.
Results
A total of 82 microRNAs were differentially expressed in erosive oral lichen planus tissues. These microRNAs are mainly linked to T helper cell differentiation that enriched in MAPK signaling pathways, as well as targeting mRNAs like MAPK11. Overexpression of miR-516a-5p resulted in a statistically significant decrease in MAPK11 mRNA level in human oral mucosal fibroblasts. Overexpression of miR-516a-5p increased TNF-α and IFN-γ levels, whereas it decreased IL-4, IL-6, IL-10, and IL-13 levels. Overexpression of miR-516a-5p enhanced both the proliferation and migration of human oral mucosal fibroblasts.
Conclusions
The miR-516a-5p overexpression in patients with erosive oral lichen planus may contribute to the imbalance of T helper 1/2 cell-associated inflammatory cytokine expression in human oral mucosal fibroblasts by targeting MAPK11 mRNA, promoting their proliferative and migratory capacities.
{"title":"Overexpression of miR-516a-5p Promotes Erosive Oral Lichen Planus: In Vitro Study Based on Bioinformatics Analyses","authors":"Yan Chen, Chen‑xi Li, Rui Xue, Zhao-xia Cong, Yi Bian, Yuan Liu","doi":"10.1002/cre2.70270","DOIUrl":"10.1002/cre2.70270","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to investigate the differentially expressed microRNAs in erosive oral lichen planus, followed by analyzing how the overexpression of identified miR-516a-5p influences human oral mucosal fibroblasts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>High-throughput sequencing using tissues from patients and healthy individuals identified varying microRNA expression profiles in erosive oral lichen planus tissues. Bioinformatics analysis subsequently revealed the enriched pathways and targeted genes involved. In vitro experiments were performed to confirm the validity of bioinformatic findings.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 82 microRNAs were differentially expressed in erosive oral lichen planus tissues. These microRNAs are mainly linked to T helper cell differentiation that enriched in MAPK signaling pathways, as well as targeting mRNAs like MAPK11. Overexpression of miR-516a-5p resulted in a statistically significant decrease in MAPK11 mRNA level in human oral mucosal fibroblasts. Overexpression of miR-516a-5p increased TNF-α and IFN-γ levels, whereas it decreased IL-4, IL-6, IL-10, and IL-13 levels. Overexpression of miR-516a-5p enhanced both the proliferation and migration of human oral mucosal fibroblasts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The miR-516a-5p overexpression in patients with erosive oral lichen planus may contribute to the imbalance of T helper 1/2 cell-associated inflammatory cytokine expression in human oral mucosal fibroblasts by targeting MAPK11 mRNA, promoting their proliferative and migratory capacities.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 6","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145848954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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