Clinical and Experimental Dental Research最新文献

{"title":"Impact of Traumatic Dental Injuries on Oral Health-Related Quality of Life Among Primary School Children in Dar es Salaam","authors":"Gloria Leo,&nbsp;Kasusu Nyamuryekung'e,&nbsp;Febronia Kokulengya Kahabuka","doi":"10.1002/cre2.70105","DOIUrl":"https://doi.org/10.1002/cre2.70105","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Traumatic dental injuries (TDIs) are a significant concern in pediatric populations, affecting children's oral health-related quality of life (OHRQoL) and overall development. This study aims to examine TDI prevalence, types, risk factors, and impacts on OHRQoL among Tanzanian school children.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Employing a cross-sectional analytical approach, the study assessed TDIs in school children aged 8–10 in Ilala district, Tanzania, using a multistage cluster sampling technique. Data collection employed structured questionnaires and clinical examinations, applying Ellis and Davey's classification and the Child Perception Questionnaire (CPQ 8–10) for TDIs and OHRQoL assessments, respectively. Statistical analyses included frequency distributions, chi-square, Mann–Whitney and Kruskal–Wallis tests, and binary logistic regression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Among 501 participants, 25.1% reported previous oral trauma with a TDI prevalence of 37.5%. Crown fractures were the most common injury (79.8%), with significant associations found between TDI prevalence and inadequate lip coverage (<i>p</i> = 0.037) and overjet (<i>p</i> &lt; 0.001). Over 60% experienced an oral impact, notably in oral symptoms (52.5%), emotional well-being (25.1%), and functional limitation (22.8%) domains. Children with TDIs showed higher OHRQoL impacts on emotional and social well-being domains.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The high prevalence of TDIs among Tanzanian school children significantly impacts their OHRQoL, emphasizing the need for comprehensive pediatric dental care approaches that encompass preventive strategies and address the multifaceted aspects of oral health.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Xylitol and Fluoride Varnish on Biofilm and Saliva in Orthodontic Patients: A Triple-Blind Randomized Clinical Trial","authors":"Neda Babanouri,&nbsp;Sarina Sahmeddini,&nbsp;Setayesh Khadang,&nbsp;Abdollah Bazargani","doi":"10.1002/cre2.70062","DOIUrl":"https://doi.org/10.1002/cre2.70062","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Fixed orthodontic appliances are associated with higher levels of plaque and saliva bacteria, which contribute to dental caries. The effects of combining xylitol and fluoride, both used in caries prevention, are uncertain. Thus, this study assessed the combined impact of fluoride and xylitol varnish on bacteria in saliva and biofilms around orthodontic brackets.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>A single-center, four-arm, parallel-group, triple-blind, randomized clinical trial was conducted. A total of 120 patients who required fixed orthodontic treatment were included. Patients were sorted into one of the following groups at random: fluoride, xylitol, combined fluoride and xylitol, and control. Biofilm and saliva sampling was performed at two intervals: T0 (first session of bracket bonding, before application of the varnish) and T1 (6 weeks after application of the varnish). The number of <i>Streptococcus mutans</i> and <i>Lactobacillus</i> was counted using the CFU method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The relative number of <i>S. mutans</i> and <i>lactobacilli</i> in saliva and biofilm significantly decreased following the application of fluoride and combined fluoride/xylitol varnish (<i>p</i> &lt; 0.05) and were more effective than xylitol varnish. There was no significant difference between fluoride and combined fluoride/xylitol varnishes regarding changes in <i>S. mutans</i> and <i>Lactobacillus</i> colonies in saliva and dental biofilms. Additionally, there was no significant difference between xylitol and the two other active varnishes regarding reduction in biofilm <i>S. mutans</i> and <i>Lactobacillus</i>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>There was no significant difference between fluoride and combined fluoride/xylitol varnishes regarding changes in the studied bacteria in saliva and dental biofilms, and they were more effective than xylitol varnish.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>The Iranian Registry of Clinical Trial identifier: IRCT20181121041713N4; https://fa.irct.ir/trial/58543</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in Tooth Development in Patients With Unilateral Cleft Lip and Palate","authors":"Marie Schwarting,&nbsp;Heinrich Wehrbein,&nbsp;Irene Schmidtmann,&nbsp;Christina Erbe,&nbsp;Susanne Wriedt","doi":"10.1002/cre2.70086","DOIUrl":"https://doi.org/10.1002/cre2.70086","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aim of this study was to investigate tooth development in patients with unilateral cleft lip and palate (UCLP) and unilateral cleft lip and alveolus (UCLA).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A retrospective case–control study was carried out; 180 panoramic radiographs (OPGs) from non-syndromic patients (160 UCLP, 20 UCLA) treated at the University Medical Center Mainz (2019–2022) were analyzed. Patients were matched to a control group by calendar age, gender, and ethnicity. Inclusion criteria were verified through clinical data, photographs, and radiographs. No follow-up was conducted for this study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The cleft group showed a significantly lower dental age compared to the control group (10.72 ± 2.65 vs. 11.41 ± 2.79; <i>p</i> = 0.017), with a mean difference of 0.69 years (95% CI: 0.13–1.25 years). Tooth development was slower on the cleft side (<i>p</i> = 0.001), and maxillary teeth lagged behind mandibular teeth (<i>p</i> &lt; 0.001). The difference in the control group was somewhat smaller, with a mean difference of 0.11 degrees of mineralization in the control group compared to 0.25 degrees of mineralization in the cleft group. In UCLP patients, significant mineralization differences were noted for the lateral incisors (<i>p</i> = 0.004), the central incisor (<i>p</i> = 0.047), and canine (<i>p</i> = 0.030).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Patients with unilateral clefts show delayed tooth development and dental age, particularly in the affected quadrant. In everyday treatment, attention should be paid to a later start of orthodontic tooth movement to avoid damaging the slower developing roots.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Success of a Mineral Trioxide Aggregate and a Pre-Mixed Bioceramic in Mature Teeth With Irreversible Pulpitis With Full Pulpotomy: A Randomized Clinical Trial","authors":"Sarang Suresh,&nbsp;Feroze A. Kalhoro,&nbsp;Priya Rani,&nbsp;Mahwish Memon","doi":"10.1002/cre2.70090","DOIUrl":"https://doi.org/10.1002/cre2.70090","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The aim of this study is to compare the clinical and radiographic success of MTA versus EBRRM in pulpotomy of permanent teeth with irreversible pulpitis without apical periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Clinical and radiographic assessments were conducted at baseline, 6 days, 6 weeks, and 6 months. After administration of anesthesia and coronal pulp removal, pulp was capped with MTA or Endo Sequence Bioceramic Root Repair, followed by restoration with a glass ionomer and resin composite.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The overall success rate for pulpotomy was 71.9%, with MTA and the bioceramic showing success rates of 32.8% and 39.1%, respectively. There was no significant relationship with the type of cavity and failure of pulpotomy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>MTA and EBBRRM are both practical choices for pulpotomy and there is no notable difference between them in the success rate and pain level. EBBRRM may be more effective in Class 1 cavities than in Class 2 cavities.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70090","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity of Different Concentrations of Silver Nanoparticles and Calcium Hydroxide for MC3T3-E1 Preosteoblast Cell Line","authors":"Farzaneh Afhkami,&nbsp;Paniz Ahmadi,&nbsp;Golriz Rostami","doi":"10.1002/cre2.70075","DOIUrl":"https://doi.org/10.1002/cre2.70075","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>With the advances in nanotechnology, nanomaterials are increasingly used in various fields due to their antibacterial properties; therefore, assessing the benefits and risks associated with the application of medicaments is imperative. This study evaluated the cytotoxicity of different concentrations of silver nanoparticles (AgNPs) and calcium hydroxide (CH) for MC3T3-E1 preosteoblast cell line.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>The MC3T3-E1 preosteoblast cells were exposed to triple antibiotic paste (TAP), AgNPs, CH, and different concentrations of AgNPs mixed with CH in 1:1, 1:2, and 1:3 ratios for 24, 48, and 72 h. Cytotoxicity was evaluated by the methyl thiazolyl tetrazolium (MTT) assay, and also the colony formation assay (CFA) was performed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>At 24 h, the TAP and AgNPs groups showed the highest and the CH-AgNPs/1:3 group had the lowest cell viability percentage in comparison to the other experimental groups. At 48 h, the TAP group showed the highest and the CH-AgNPs/1:3 group showed the lowest cell viability. At 72 h, the AgNPs and CH groups showed the highest viability, while the lowest viability was noted in the CH-AgNPs/1:3 and CH-AgNPs/1:2 groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>AgNPs showed the least cytotoxic effects in all periods. The addition of AgNPs to CH increases the cytotoxic effects of CH on experimental cells. After 48 and 72 h, CH-AgNPs/1:1 showed significantly higher cell viability in comparison to higher concentrations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radicular Cysts and Periapical Granulomas: Data Documentation for 696 Cases and Findings on Fibrosis, and Porphyromonas gingivalis and Fusobacterium nucleatum in These Lesions","authors":"Sirke Virkkunen,&nbsp;Terhi Kaarela,&nbsp;Merja Laine,&nbsp;Auli Suominen,&nbsp;Jaana Hagström,&nbsp;Timo Sorsa,&nbsp;Caj Haglund","doi":"10.1002/cre2.70098","DOIUrl":"https://doi.org/10.1002/cre2.70098","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The objective of our study was to re-evaluate periapical lesions, including radicular cysts (RCs) and periapical granulomas (PGs) for locations, histopathological features, and degree of fibrosis in relation to the inflammatory response. In addition, we examined the presence of <i>Porphyromonas gingivalis</i> (Pg) and <i>Fusobacterium nucleatum</i> (Fn) since both are widely recognized pathogens in periodontal infections.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We re-evaluated samples of RCs and PGs (<i>n</i> = 728) and collected data for analyses by IBM's SPSS Statistics. Among these samples, we stained 93 samples to determine the immunoexpression of Pg and Fn. For immunostaining, we used Gingipain R1 antibody for Pg and Rabbit anti-Fn antibody for Fn.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Fibrosis is associated with mild inflammation. We found a significant positive correlation between Pg and Fn. Thus, these pathogens are likely to occur together in periapical inflammatory lesions. We additionally noted that these periodontopathic pathogens are more likely to be present in RCs than in PGs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Asymptomatic radiologically diagnosed periapical lesions may not necessarily need root canal retreatment in healthy patients since these lesions may represent scar tissue rather than active apical periodontitis. Clinical and radiological follow-up is still needed. Yet, periapical lesions, especially cysts, may contain dystopic periodontopathic pathogens, and Pg and Fn often occur together in periapical lesions.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Outcome and Predictors of Customized Polyetheretherketone Post-and-Cores for Residual Root and Crown: A Retrospective Study","authors":"Xin Wang,&nbsp;Chen Liu,&nbsp;Yuchen Liu,&nbsp;Dan Ma,&nbsp;Ruifang Ren,&nbsp;Mingxing Zhang,&nbsp;Jiawen Guo,&nbsp;Yimin Zhao,&nbsp;Dongmei Li,&nbsp;Shizhu Bai","doi":"10.1002/cre2.70066","DOIUrl":"https://doi.org/10.1002/cre2.70066","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This retrospective, non-interventional study aimed to evaluate the longevity and factors influencing the success of custom-fabricated polyetheretherketone (PEEK) post-and-cores in endodontically treated teeth (ETT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>During the observation period (2019–2022), 63 patients received 100 customized PEEK post-and-cores. Clinical outcomes were analyzed based on the following parameters: age, gender, tooth type, tooth position, proximal contacts, remaining cavity walls, antagonist contacts, and final restoration. Kaplan–Meier analysis was used for the survival probability. Log-rank tests were used to identify univariate associations between failure rates and other potential factors. Cox regression was used to assess the risk of failure and determine possible risks between the identified factors at a cut-off point <i>p</i>-value (0.25 in univariate analysis).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The median follow-up duration was 20.62 months, with a maximum of 40.57 months. 93 restorations were judged as successful and 99 teeth survived. The main failure type was decementation of the restoration (<i>n</i> = 4). The annual failure rate was 3.4%. Gender, remaining tooth structure, and final restoration were found to be correlated with success rates in the preliminary univariate analysis (Log-rank tests) to determine the association between failure rates and potential factors. Multifactorial survival analysis (Cox regression) showed that teeth with coronal walls had a significantly lower failure risk than deprived teeth, even with the ferrule effect. (HR = 0.04; 95% CI for HR = 0.01–0.29; <i>p</i> = 0.002).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>PEEK post-and-cores adapt well to clinical restorative needs and offer favorable short-term clinical outcomes. The remaining cavity wall was a significant success rate predictor.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Effect of High-Translucent Zirconia Thickness and Substrate Shade on the Final Color of the Restoration","authors":"Mahnaz Hatami,&nbsp;Elham Jalali,&nbsp;Mohamad Hossein Lotfi Kamran,&nbsp;Alireza Danesh Kazemi,&nbsp;Amirhossein Fathi","doi":"10.1002/cre2.70091","DOIUrl":"https://doi.org/10.1002/cre2.70091","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>The purpose of this study was to evaluate the effect of high-translucent zirconia thickness and substrate shade on the final color of the restoration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>A total of 60 high-translucent monolithic zirconia disks were prepared using a CAD/CAM system. They were placed on composite substrates with A2, A3.5, and C3 colors (<i>n</i> = 10). The color differences (Δ<i>E</i>) of the ceramic disks on the A3.5 and C3 substrate compared to the control group (A2) were calculated and compared with acceptable thresholds (Δ<i>E</i> = 5.5) and perceptible thresholds (Δ<i>E</i> = 2/6). A one-sample <i>t</i>-test and repeated measures ANOVA were used to analyze the data statistically.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The highest Δ<i>E</i> values were observed when comparing two zirconia disk thicknesses on the A3.2 substrate without cement (Δ<i>E</i> = 5/65). The lowest value of Δ<i>E</i> compared to the control group (A2) was related to the disk with 1 mm thickness on the A3.5 substrate (Δ<i>E</i> = 2/54), and the highest value was for the disk with 0.6 mm thickness on the C3 substrate (Δ<i>E</i> = 4/88).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Using the zirconia disk with a 1 mm thickness on the C3 structure and a disk with a 0.6 mm thickness on the A3.5 structure with the presence of F2 cement can achieve an acceptable color difference (value of 5.5).</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the 53-Year Epidemiological Trends of Oral Squamous Cell Carcinoma (OSCC) in Northeastern Iran","authors":"Nasrollah Saghravanian,&nbsp;Yasamin Tajdini,&nbsp;Pooya Saeedi,&nbsp;Mahsa Ghorbani","doi":"10.1002/cre2.70092","DOIUrl":"https://doi.org/10.1002/cre2.70092","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>This study aimed to assess the epidemiological trends of oral squamous cell carcinoma (OSCC) in Northeast Iran over a 53-year period.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>In this retrospective study, we obtained data on OSCC cases in Northeast Iran spanning 53 years (1970–2022), including demographic details, such as age, sex, site, and histopathological grade. Descriptive analysis was conducted, and frequencies were compared using the chi-squared test to evaluate differences between sexes, age groups, cancer sites, and histopathological grades. Additionally, we assessed the associations of each OSCC site with age, sex, and histopathological grade. Statistical significance was set at <i>p</i> &lt; 0.05, with a confidence interval of 95%.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>This study examined 13,769 cases, among which 507 were diagnosed with OSCC, accounting for 3.68% of the total cases. The mean age was 59.27 ± 14.94 years, with a significantly higher prevalence of OSCC among individuals older than 50 years (<i>p</i> &lt; 0.001). There were nearly equal proportions of males and females, with the tongue being the most common site (28.4%), followed by the gingiva (26.4%) and buccal mucosa (19.5%). Grade 1 (well differentiated) OSCC was significantly more prevalent (54%) than other grades. There was a significant association between sex and tongue SCC in females (<i>p</i> = 0.006) and between sex and lip SCC in males (<i>p</i> = 0.008). Prevalence in individuals above 50 was significant for the tongue, buccal mucosa, gingiva, and lip (<i>p</i> = 0.001, &lt; 0.001, &lt; 0.001, 0.028, respectively). In the tongue, buccal mucosa, gingiva, and floor of the mouth, grade 1 was significantly more prevalent (<i>p</i> &lt; 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our study revealed that OSCC predominantly affects individuals older than 50 years, with nearly equal prevalence between sexes. The most commonly affected sites are the tongue and gingiva, often exhibiting grade 1 histopathological findings. Enhanced community awareness of risk factors and regular oral examinations are essential for reducing the incidence of OSCC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Vesicles Derived From Lipopolysaccharide-Challenged Gingival Fibroblast Reveal Distinct miRNA Expression Patterns Associated With Reduced Cancer Survival","authors":"Daniel Diehl,&nbsp;Charlotte Lauren Brauer,&nbsp;Hagen S. Bachmann,&nbsp;Daniel Pembaur,&nbsp;Patrick Philipp Weil,&nbsp;Anton Friedmann","doi":"10.1002/cre2.70099","DOIUrl":"https://doi.org/10.1002/cre2.70099","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>Periodontitis is a prevalent inflammatory disease with established systemic implications. Extracellular vesicles (EVs) have emerged as key mediators of intercellular communication, potentially linking periodontitis to systemic diseases. However, the molecular cargo of EVs from inflamed periodontal cells remains poorly characterized. This study investigates the EV cargo of human gingival fibroblasts (hGF-hTERT) following lipopolysaccharide (LPS) stimulation and explores their potential role in cancer progression.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>EVs were isolated from LPS-treated and untreated fibroblasts via ultracentrifugation. Dynamic light scattering and scanning electron microscopy characterized EV size and morphology. RNA sequencing identified differentially expressed miRNAs, validated by qPCR. Functional pathway enrichment and in-silico analyses using The Cancer Genome Atlas (TCGA) were performed to assess EV-associated miRNA impact on tumorigenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>EV size and concentration remained unchanged after LPS stimulation. However, LPS-derived EVs exhibited a 2.6-fold increase in miRNA content, with three significantly upregulated miRNAs: miR-146a-5p, miR-486-5p, and miR-451a. Functional enrichment analysis revealed their involvement in inflammation, immune modulation, and cancer pathways. In vitro, LPS-derived EVs significantly enhanced prostate cancer (LnCap) cell proliferation. TCGA analysis linked the upregulated miRNAs to poor cancer prognosis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>LPS stimulation alters the miRNA cargo of gingival fibroblast-derived EVs, enhancing pathways associated with inflammation and cancer progression. These findings suggest a mechanistic role for periodontal EVs in systemic disease pathogenesis, warranting further investigation into their diagnostic and therapeutic potential.</p>\u0000 </section>\u0000 </div>","PeriodicalId":10203,"journal":{"name":"Clinical and Experimental Dental Research","volume":"11 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cre2.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}