Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献_第10页

Developmental toxicity of functionalized polystyrene microplastics and their inhibitory effects on fin regeneration in zebrafish 功能化聚苯乙烯微塑料的发育毒性及其对斑马鱼鳍再生的抑制作用。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-09-19 DOI: 10.1016/j.cbpc.2025.110358

Qian Li , Wenjing Cheng , Hongyu Wang , Jun Chen , Yueyun Qin , Ruozhu Sheng , Shaohong Peng , Zaitian Li , Tao Lu , Liwei Sun

The hazardous effects of microplastics on aquatic biota have been extensively documented. However, the impact of surface functionalization on the toxicological profiles of microplastics, particularly in fish, requires further investigation. In the present study, the effects of polystyrene microplastics (50–100 nm in diameter), either non-functionalized or functionalized with carboxyl (-COOH) or amino (−NH₂) groups, on zebrafish were explored. Exposure to all three types of microplastics (0.1, 1, and 10 mg/L) resulted in developmental toxicity in zebrafish at early life stages. This included increased mortality, reduced larval body length, decreased heart rate, impaired swimming ability, as well as disruptions in redox homeostasis, nervous system development, and immune responses. Moreover, all types of microplastics (1 mg/L) inhibited the regenerative capacity of larval zebrafish after caudal fin amputation, both morphologically and functionally. This inhibition was likely due to alterations in immune response, excessive production of reactive oxygen species, and disruptions in the signaling networks that regulate fin regeneration. Among the tested microplastics, amino-modified particles generally exhibited greater developmental toxicity and stronger inhibitory effects on fin regeneration. Our findings confirm that the functionalization of microplastics plays a crucial role in their interactions with biological systems and highlight concerns regarding the potential effects of functionalization, whether intentional or resulting from natural weathering processes.

微塑料对水生生物群的有害影响已被广泛记载。然而，表面功能化对微塑料毒理学特征的影响，特别是对鱼类的影响，需要进一步研究。在本研究中，研究了聚苯乙烯微塑料（直径50-100 nm），无论是非官能团还是羧基（-COOH）或氨基（-NH2）官能团，对斑马鱼的影响。暴露于所有三种类型的微塑料（0.1、1和10 mg/L）会导致斑马鱼在生命早期出现发育毒性。这包括死亡率增加，幼虫体长缩短，心率下降，游泳能力受损，以及氧化还原稳态，神经系统发育和免疫反应的破坏。此外，所有类型的微塑料（1 mg/L）在形态和功能上都抑制了斑马鱼尾鳍切除后的再生能力。这种抑制可能是由于免疫反应的改变、活性氧的过量产生以及调节鳍再生的信号网络的中断。在所测试的微塑料中，氨基修饰颗粒普遍表现出更大的发育毒性和更强的鳍再生抑制作用。我们的研究结果证实，微塑料的功能化在它们与生物系统的相互作用中起着至关重要的作用，并强调了功能化的潜在影响，无论是故意的还是自然风化过程的结果。

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引用次数: 0

The effects of pulse exposures to road salt at various stages of early development in rainbow trout (Oncorhynchus mykiss) 脉冲暴露于道路盐对虹鳟鱼早期发育不同阶段的影响。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-08-27 DOI: 10.1016/j.cbpc.2025.110334

Carley E. Winter , Clare L. Kilgour , Colin J. Brauner , Patricia M. Schulte , Chris M. Wood

Salmonids spawn in freshwater streams including those in urban areas that are impacted by human activities. In the Vancouver region of British Columbia, Canada, the extensive use of road salt (primarily NaCl) is associated with frequent 24-h “pulses” of salt in streams, some of which may exceed the provincial acute guideline for maximum chloride concentrations (600 mg L⁻¹ Cl⁻) by up to 11-fold. For some salmonids, road salting coincides with critical developmental stages, as many species spawn between October and January. We explored the concentration-dependent effects of a 24-h salt pulse (600–9600 mg L⁻¹ Cl⁻) on salmonid development using rainbow trout (Oncorhynchus mykiss). Salt pulses were imposed at one of three developmental time points: <1 h post-fertilization, the eyed-stage or 7 days post-hatch. Significant mortality occurred only in the <1 h post-fertilization treatment, at 2400, 4800 and 9600 mg L⁻¹ Cl⁻, all environmentally relevant salt concentrations. Significant differences in whole-embryo ion concentrations at the end of the salt exposure and at the eyed-stage (17 days post-salt exposure) indicated lasting ionoregulatory effects on embryos. Co-exposure to CaCO₃ during the salt pulse, at a level that increased dissolved Ca²⁺ by 2-to 3-fold in the ion poor Vancouver water, greatly reduced mortality and altered whole-embryo ion levels. These findings support the need for site-specific water quality guidelines, as toxicity varies with water's ionic composition. This research also highlights the need for improved road salting practices to reduce salt contamination and its potential adverse effects on developing salmonids.

鲑鱼在淡水溪流中产卵，包括受人类活动影响的城市地区的淡水溪流。在加拿大不列颠哥伦比亚省的温哥华地区，道路盐（主要是NaCl）的广泛使用与溪流中盐的频繁24小时“脉冲”有关，其中一些可能超过最高氯化物浓度的省级急性指南（600 mg L-1 Cl-）高达11倍。对于一些鲑科鱼类来说，道路上的盐腌正好是它们发育的关键阶段，因为许多物种在10月到1月之间产卵。研究了24小时盐脉冲（600-9600 mg L-1 Cl-）对虹鳟（Oncorhynchus mykiss）发育的浓度依赖性影响。在三个发育时间点之一施加盐脉冲：-1 Cl-，所有与环境相关的盐浓度。盐暴露结束时和眼期（盐暴露后17 天）全胚胎离子浓度的显著差异表明，盐对胚胎有持续的离子调节作用。在盐脉冲期间共同暴露于CaCO3，在离子贫乏的温哥华水中，溶解Ca2+的水平增加了2至3倍，大大降低了死亡率并改变了全身离子水平。这些发现支持了特定地点水质指南的必要性，因为毒性随水的离子组成而变化。这项研究还强调了改善道路盐化做法的必要性，以减少盐污染及其对鲑鱼发育的潜在不利影响。

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引用次数: 0

Artificial light at night disrupts fertility in Drosophila melanogaster 夜间的人造光会干扰果蝇的生育能力。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-09-06 DOI: 10.1016/j.cbpc.2025.110349

Margherita Martelli , Raffaella Lazzarini , Francesco Piva , Gianmaria Salvio , Alessandro Ciarloni , Lory Santarelli , Massimo Bracci

Artificial light at night (ALAN) can disrupt numerous biological processes, and is increasingly studied in animal models. Here, we evaluated the impact of red and blue ALAN on Drosophila melanogaster, focusing on fertility, development, circadian rhythms, and gene expression. All results were compared to those of a control group maintained under a 12 h white light/12 h dark cycle.

Red ALAN exposure increased the number of eggs laid but reduced the hatching rate and shortened the larval period. Conversely, blue ALAN led to fewer eggs laid, fewer emerging adults, and lower hatching success. Significant alterations in circadian rhythm and the sleep–wake cycle were observed in flies exposed to both red and blue ALAN, including a reduction in mean locomotor activity over 24 h and during the daytime period, increased sleep duration during the day, and reduced sleep duration at night. Effects were more pronounced under blue ALAN, which disrupted circadian rhythm by eliminating morning and evening activity peaks and increasing nocturnal activity. Gene expression analyses revealed that red ALAN upregulated ecdysone-induced protein 74EF (E74) and the ecdysone receptor (EcR) expression in adults, while juvenile hormone binding protein 1 (Jhbp1) was elevated under both light conditions. In larvae, both ALAN spectra increased expression of E74 and Jhbp1.

These findings demonstrate that red and blue ALAN can significantly disrupt fertility and development in Drosophila melanogaster. Given the rising prevalence of light pollution and night-shift work, further studies are needed to investigate ALAN-related reproductive impairments in other animals, including vertebrates and humans.

夜间人造光（ALAN）可以破坏许多生物过程，并且越来越多地在动物模型中进行研究。在这里，我们评估了红色和蓝色ALAN对黑腹果蝇的影响，重点是生育力、发育、昼夜节律和基因表达。所有结果都与对照组在12 h白光/12 h暗循环下的结果进行了比较。赤色艾伦暴露增加了产卵数，但降低了孵化率，缩短了幼虫期。相反，蓝色ALAN导致产卵量减少，成虫数量减少，孵化成功率降低。在暴露于红色和蓝色ALAN的果蝇中，观察到昼夜节律和睡眠-觉醒周期的显著变化，包括24 h和白天平均运动活动减少，白天睡眠时间增加，夜间睡眠时间减少。蓝色ALAN的效果更明显，它通过消除早晚活动高峰和增加夜间活动来扰乱昼夜节律。基因表达分析显示，红色ALAN上调蜕皮激素诱导蛋白74EF （E74）和蜕皮激素受体（EcR）的表达，而幼体激素结合蛋白1 （Jhbp1）在两种光照条件下均升高。在幼虫中，ALAN光谱均增加了E74和Jhbp1的表达。这些发现表明，红色和蓝色ALAN可以显著破坏果蝇的生育和发育。鉴于光污染和夜班工作的日益普遍，需要进一步研究包括脊椎动物和人类在内的其他动物与alan相关的生殖障碍。

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引用次数: 0

Effects of short-term exposure to environmentally relevant pesticides mixture on morphological alterations, oxidative-nitrative stress biomarkers, cellular apoptosis, and antioxidant expression in kidneys of goldfish 短期暴露于环境相关农药混合物对金鱼肾脏形态改变、氧化-硝化应激生物标志物、细胞凋亡和抗氧化剂表达的影响

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-09-01 DOI: 10.1016/j.cbpc.2025.110337

Esmirna Cantu , Md Saydur Rahman

Chemical stressors are pervasive, affecting both terrestrial and aquatic environments. The continual influx of these toxins is damaging ecosystems and the organisms that inhabit them. The abundance of environmental toxins makes aquatic habitats inhospitable for aquatic life. These chemical stressors consistently disrupt the life processes of aquatic organisms, particularly their physiological functions. This study examined on the effects of environmentally relevant pesticides mixture (low-dose and high-dose: S-metolachlor: 2.4 go/L, 12 μg/L; linuron: 2.0 μg/L, 10 μg/L; isoproturon: 1.2 μg/L, 6.0 μg/L; tebucanozole: 1.2 μg/L, 6.0 μg/L; aclonifen: 0.8 μg/L, 4.0 μg/L; atrazine: 0.4 μg/L, 2.0 μg/L; pendimethalin: 0.4 μg/L, 2.0 μg/L, and azinphos-methyl: 0.8 μg/L, 4.0 μg/L) on tissue morphology, cellular apoptosis, and nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), renin, superoxide dismutase (SOD), and catalase (CAT) expression in the kidneys of goldfish (Carassius auratus) exposed for one week under controlled laboratory conditions. Histopathological analysis revealed severe tissue damage in the kidneys, while silver staining identified melano-macrophage centers, and immunohistochemistry and real-time PCR shed light on the expression of molecular biomarkers in these tissues. Exposure to pesticide mixtures caused fish to exhibit glomerular shrinkage, enlargement of Bowman's space, and degradation of glomerular cells. Moreover, the expression of renin, DNP, NTP, SOD, and apoptotic nuclei increased in kidney tissues, while CAT expression decreased. Overall, our findings indicate that exposure of goldfish to an environmentally relevant pesticide mixture leads to increased cellular damage and altered osmoregulatory and antioxidant enzyme expressions, which may impair physiological functions, including growth, reproduction, and development in teleost fish.

化学压力源无处不在，影响着陆地和水生环境。这些毒素的不断涌入正在破坏生态系统和栖息在其中的生物。丰富的环境毒素使水生生物的栖息地不适宜生存。这些化学应激源不断破坏水生生物的生命过程，特别是它们的生理功能。这项研究检查了相关环保农药混合物的影响(低剂量和高剂量:S-metolachlor: 2.4 / L, 12 μg / L;利谷隆: 2.0μg / L, 10 μg / L; isoproturon: 1.2μg / L, 6.0μg / L; tebucanozole: 1.2μg / L, 6.0μg / L; aclonifen: 0.8μg / L, 4.0μg / L;莠去津: 0.4μg / L, 2.0μg / L;在实验室条件下，二甲萘：0.4 μg/L, 2.0 μg/L，甲基氮磷：0.8 μg/L, 4.0 μg/L，对鲫鱼（Carassius auratus）肾脏组织形态、细胞凋亡及硝基酪氨酸蛋白（NTP）、二硝基苯蛋白（DNP）、肾素、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）表达的影响。组织病理学分析显示肾脏严重的组织损伤，而银染色鉴定了黑素巨噬细胞中心，免疫组织化学和实时PCR揭示了这些组织中分子生物标志物的表达。暴露于农药混合物中使鱼表现出肾小球收缩、鲍曼间隙增大和肾小球细胞降解。肾组织中肾素、DNP、NTP、SOD、凋亡核表达升高，CAT表达降低。总的来说，我们的研究结果表明，金鱼暴露于与环境相关的农药混合物中会导致细胞损伤增加，渗透调节和抗氧化酶表达改变，这可能会损害硬骨鱼的生理功能，包括生长、繁殖和发育。

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引用次数: 0

Transcriptomic profiling reveals tetrabromobisphenol A-induced dysregulation of hepatic lipid homeostasis in common carp (Cyprinus carpio) 转录组学分析揭示了四溴双酚a诱导的鲤鱼肝脏脂质稳态失调。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-08-26 DOI: 10.1016/j.cbpc.2025.110336

Yimin Li , Chunnuan Zhang , Zihao Meng , Mengkang You , Huajuan Shi

TBBPA, a ubiquitously distributed brominated flame retardant, poses significant ecological risks due to its bioaccumulative potential and toxicity. Previous investigations have predominantly focused on untargeted metabolomics and lipidomics to characterize the effects of TBBPA on metabolic end products. However, limited data are available regarding the mechanisms underlying TBBPA-induced disruption of lipid metabolism in aquatic organisms. This study systematically investigated the dose-dependent effects of TBBPA on lipid metabolism in common carp through integrated biochemical, histochemical evaluations, and transcriptomic profiling. Fish were exposed to environmentally relevant TBBPA concentrations (0, 0.005, 0.05, and 0.5 mg/L) for a 14-day duration. Serum biochemical analyses revealed dose-dependent hyperlipidemia, manifested by elevated TG and T-CHO levels, suppressed SOD and CAT activities, and increased MDA levels. Oil Red O staining demonstrated hepatic lipid droplet accumulation despite no significant (P > 0.05) alterations in intrahepatic TG/T-CHO content, with the 0.5 mg/L group exhibited the most severe histopathological phenotype. Transcriptomic profiling identified the AMPK and PPAR signaling pathways as pivotal regulators of TBBPA-induced metabolic dysregulation. Under low-dose TBBPA exposure (0.05 mg/L), lipid accumulation triggered compensatory activation of the AMPK/PPAR-α/CPT1 axis, enhancing fatty acid β-oxidation to partially counteract lipid deposition. Conversely, high-dose TBBPA (0.5 mg/L) disrupted lipid metabolism through coordinated upregulation of genes associated with lipid synthesis, transport, and storage, culminating in pathological lipid deposition. This study provides critical insights into the molecular cascades underlying TBBPA-induced metabolic dysregulation in aquatic species, highlights the dose-dependent disruption of lipid homeostasis.

TBBPA是一种普遍存在的溴化阻燃剂，具有生物蓄积性和毒性，对生态环境具有重大危害。以前的研究主要集中在非靶向代谢组学和脂质组学上，以表征TBBPA对代谢终产物的影响。然而，关于tbbpa诱导的水生生物脂质代谢破坏的机制的数据有限。本研究通过综合生化、组织化学评估和转录组学分析，系统地研究了TBBPA对鲤鱼脂质代谢的剂量依赖性作用。鱼暴露于与环境相关的TBBPA浓度（0、0.005、0.05和0.5 mg/L）中14天。血清生化分析显示剂量依赖性高脂血症，表现为TG和T-CHO水平升高，SOD和CAT活性抑制，MDA水平升高。尽管肝内TG/T-CHO含量无显著变化（P > 0.05），但油红O染色显示肝脂滴积聚，其中0.5 mg/L组表现出最严重的组织病理表型。转录组学分析发现AMPK和PPAR信号通路是tbbpa诱导的代谢失调的关键调节因子。低剂量TBBPA暴露（0.05 mg/L）下，脂质积累触发AMPK/PPAR-α/CPT1轴代偿激活，增强脂肪酸β-氧化，部分抵消脂质沉积。相反，高剂量TBBPA（0.5 mg/L）通过协调上调与脂质合成、运输和储存相关的基因来破坏脂质代谢，最终导致病理性脂质沉积。这项研究为水生物种中tbbpa诱导的代谢失调的分子级联提供了重要的见解，强调了脂质稳态的剂量依赖性破坏。

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引用次数: 0

Unveiling excessive feed-sources copper-induced ileitis in chickens: Insights into tight junction damage and ROS/NLRP3/pyroptosis axis 揭示过量饲料来源铜诱导的鸡回肠炎：对紧密连接损伤和ROS/NLRP3/焦亡轴的见解。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-09-13 DOI: 10.1016/j.cbpc.2025.110357

Boran Zhou , Yufei Cao , Yingxue Zhang, Mingwei Xing, Yu Wang

Copper, widely used as a growth promoter and antibacterial agent, is commonly added to livestock and poultry feed. However, this widespread use leads to its accumulation in the animals' bodies, resulting in intestinal toxicity. The specific mechanisms of copper-induced ileitis in broilers remain unclear. In this study, broilers were fed diets containing 0, 100, or 300 mg/kg CuSO₄ over a five-week period. Results showed that the high‑copper group (300 mg/kg) exhibited significant suppression of antioxidant defenses, including reduced heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO1), total superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels, while catalase (CAT) activity was paradoxically elevated. Notably, NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome complex activation (NLRP3, apoptosis-associated speck-like protein containing a CARD [ASC], Caspase-1) and upregulated pyroptosis markers (Caspase-1, interleukin-1β [IL-1β]) indicated reactive oxygen species (ROS)–NLRP3 axis involvement. Concurrently, pro-inflammatory mediators (interleukin-7 [IL-7], interleukin-17 [IL-17], inducible nitric oxide synthase [iNOS], tumor necrosis factor-α [TNF-α]) were dysregulated, accompanied by diminished expression of tight junction proteins (zonula occludens-1 [ZO-1], Claudin-3, Occludin) and inhibition of the Wnt/β-catenin pathway. These findings demonstrate that copper-induced intestinal inflammation and pyroptosis are driven by ROS–NLRP3 axis activation, while simultaneously compromising mucosal barrier integrity. This study elucidates critical mechanisms of copper toxicity in broilers and highlights risks associated with excessive copper exposure.

铜被广泛用作生长促进剂和抗菌剂，通常添加到畜禽饲料中。然而，这种广泛使用导致其在动物体内积累，导致肠道毒性。铜诱发肉鸡回肠炎的具体机制尚不清楚。在本研究中，肉鸡在5周内分别饲喂含有0、100或300 mg/kg硫酸铜的饲粮。结果表明，高铜组（300 mg/kg）显著抑制了抗氧化防御，包括血红素氧化酶-1 （HO-1）、NAD(P)H醌脱氢酶1 （NQO1）、总超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）水平降低，而过氧化氢酶（CAT）活性却升高。值得注意的是，nod样受体家族pyrin结构域包含3 （NLRP3）炎性体复合物激活（NLRP3，凋亡相关斑点样蛋白含有CARD [ASC]， Caspase-1）和上调的焦亡标志物（Caspase-1，白细胞介素-1β [IL-1β]）表明活性氧(ROS)-NLRP3轴参与。同时，促炎介质（白细胞介素-7 [IL-7]、白细胞介素-17 [IL-17]、诱导型一氧化氮合酶[iNOS]、肿瘤坏死因子-α [TNF-α]）出现异常，并伴有紧密连接蛋白（闭塞带-1 [ZO-1]、Claudin-3、Occludin）表达减少，Wnt/β-catenin通路受到抑制。这些发现表明，铜诱导的肠道炎症和焦亡是由ROS-NLRP3轴激活驱动的，同时损害了粘膜屏障的完整性。本研究阐明了肉仔鸡铜中毒的关键机制，并强调了过量铜暴露的相关风险。

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引用次数: 0

Neuroprotective effect of ferulic acid in valproic acid induced autism like behaviour in zebrafish via modulation of PI3K/AKT/mTOR pathway 阿威酸通过调节PI3K/AKT/mTOR通路对丙戊酸诱导的斑马鱼自闭症样行为的神经保护作用

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-09-14 DOI: 10.1016/j.cbpc.2025.110347

Dhrita Chatterjee , Shamsher Singh

Valproic acid (VPA), a widely used antiepileptic and mood stabilizing drug, is known to induce autism-like features when administered during neurodevelopment. Recent evidence suggests that VPA exposure during adulthood may also elicit autism spectrum disorder (ASD)-like features by altering key signalling pathways, such as phosphoinositide 3-kinase/AKT/mammalian target of rapamycin (PI3K-AKT-mTOR), and cause behavioural and neuromorphological deficits. The study explored the neuroprotective properties of ferulic acid (FA) in VPA-induced cognitive and behavioural impairments. Zebrafish were exposed to VPA at 500 μM for four consecutive days to induce ASD-like features. After 4 days of VPA exposure, they were treated with FA (50, 100, and 200 mg/kg) and risperidone (0.5 mg/kg) for 4 days. Behavioural (T-maze, Novel Tank Driving Test (NTDT), and social interaction), biochemical (oxidative markers), molecular changes (PI3K, mTOR by ELISA, and AKT by immunohistochemistry), and histopathological analyses were performed to confirm the neuroprotective properties of ferulic acid (FA). VPA (500 μM) exposure significantly deteriorated behavioural and molecular alteration levels (p < 0.001 vs. normal control group) in zebrafish. However, FA (100 and 200 mg/kg) significantly improved cognitive and behavioural alterations, as well as oxidative marker and neurotransmitter levels (p < 0.05 vs. VPA group) in zebrafish. Treatment also improved histopathological changes and AKT levels (p < 0.001 vs. the VPA group) in zebrafish. Our results demonstrated that the therapeutic effect of FA in VPA induced autism like symptoms in zebrafish was mediated by its antioxidant, anti-inflammatory, and anti-apoptotic properties through modulation of the PI3K-AKT-mTOR pathway, offering a promising therapeutic strategy for ASD-like symptoms.

丙戊酸（VPA）是一种广泛使用的抗癫痫和情绪稳定药物，已知在神经发育期间服用丙戊酸会诱发自闭症样特征。最近的证据表明，成年期暴露于VPA也可能通过改变关键信号通路，如磷酸肌肽3-激酶/AKT/哺乳动物雷帕霉素靶点（PI3K-AKT-mTOR），引发自闭症谱系障碍（ASD）样特征，并导致行为和神经形态学缺陷。本研究探讨阿魏酸（FA）对vpa诱导的认知和行为障碍的神经保护作用。将斑马鱼暴露在500 μM的VPA中，连续4天诱导asd样特征。VPA暴露4 天后，分别给予FA（50、100和200 mg/kg）和利培酮（0.5 mg/kg）治疗4 天。通过行为学（t迷宫、新型坦克驾驶测试（NTDT）和社会互动）、生化（氧化标志物）、分子变化（ELISA检测PI3K、mTOR和免疫组化检测AKT）和组织病理学分析来证实阿魏酸（FA）的神经保护作用。VPA（500 μM）暴露显著恶化行为和分子改变水平(p

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引用次数: 0

Effects of cypermethrin on the clams Ruditapes philippinarum (A. Adams & Reeve, 1850) 氯氰菊酯对菲律宾蛤的影响（A. Adams & Reeve, 1850）。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-10-02 DOI: 10.1016/j.cbpc.2025.110366

Lihua Qi , Chengang Hua , Zongqi Ye , Xu Shen , Yuefeng Cai , Xin Shen

Cypermethrin, highly toxic to aquatic life, impacts Ruditapes philippinarum (A. Adams & Reeve, 1850), an economically valuable species. Thus, studying its toxicity mechanism to R. philippinarum is crucial. In this research, R. philippinarum was exposed to 5000 ng/L cypermethrin and sampled on days 0, 1, 3, 6, 10, 15, and 21 to assess enzymatic activities and conduct transcriptome sequencing. The enzymatic activity results showed that cypermethrin could increase the activity of the ethoxyresorufin-O-deethylase (EROD), turn on the antioxidant defenses, cause an initial increase in total antioxidant capacity (T-AOC) and then a decrease, increase the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), and inhibit superoxide dismutase (SOD). Furthermore, the contents of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) increased in the body at 10, 15, and 21 days of exposure. Transcriptome sequencing was carried out to analyze the responses of cypermethrin stress for 1 day and 21 days. Differentially expressed genes (DEGs) were linked to detoxification metabolism and oxidative stress, according to Gene Ontology (GO) enrichment analysis. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis demonstrated that cypermethrin had toxic effects through key factors affecting detoxification metabolism, oxidative stress, immune response, and apoptosis-related pathways. This study gives insights into the harmful mechanism of cypermethrin stress on R. philippinarum, as well as a theoretical basis for assessing the ecological danger of cypermethrin in Chinese coastal waters.

氯氰菊酯对水生生物有剧毒，对菲律宾Ruditapes philippinarum （A. Adams & Reeve, 1850）具有经济价值。因此，对其毒性机制的研究具有重要意义。本研究采用5000 ng/L的氯氰菊酯对菲律宾褐藻进行处理，并于第0、1、3、6、10、15和21天取样，评估其酶活性并进行转录组测序。酶活性研究结果表明，氯氰菊酯能提高乙氧基间苯二酚- o -去乙基酶（EROD）活性，开启抗氧化防御，使总抗氧化能力（T-AOC）先升高后降低，提高过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性，抑制超氧化物歧化酶（SOD）活性。此外，暴露后10、15和21 天，体内丙二醛（MDA）和8-羟基脱氧鸟苷（8-OHdG）含量增加。转录组测序分析1 d和21 d对氯氰菊酯胁迫的响应。根据基因本体（GO）富集分析，差异表达基因（DEGs）与解毒代谢和氧化应激有关。此外，京都基因与基因组百科（KEGG）富集分析表明，氯氰菊酯通过影响解毒代谢、氧化应激、免疫反应和细胞凋亡相关途径的关键因素具有毒性作用。本研究揭示了氯氰菊酯胁迫对菲律宾绒螯蟹的危害机理，为我国沿海水域氯氰菊酯生态危害评价提供了理论依据。

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引用次数: 0

Fish cancer and its assistance to human cancer research: A fresh perspective 鱼类癌症及其对人类癌症研究的帮助：一个新的视角。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-10-13 DOI: 10.1016/j.cbpc.2025.110369

Hao Dan , Wentong Wang , Xuhong Chen , Mariam N. Goda , Mohamed S. Kisha , Quanquan Cao

Cancer is a complex disease affecting various species, including fish, which have emerged as valuable models for studying cancer mechanisms. Recent studies highlight the similarities and differences between fish and mammalian tumors, offering insights for novel human cancer therapies. Fish models, particularly zebrafish, replicate key cancer phenotypes and molecular pathways seen in humans, making them powerful tools for cancer research. This review explores the transition from fish to human cancer studies, focusing on the molecular basis, diagnosis, and treatment of fish cancer. Key findings include the role of blood vessel formation in fish tumors and the application of zebrafish tumor models to understand human cancer. These studies provide new perspectives and technologies for human cancer research, though challenges remain in fully leveraging fish models for future therapeutic advancements.

癌症是一种影响各种物种的复杂疾病，包括鱼类，它们已成为研究癌症机制的有价值的模型。最近的研究强调了鱼类和哺乳动物肿瘤之间的异同，为新的人类癌症治疗提供了见解。鱼类模型，特别是斑马鱼，复制了在人类中看到的关键癌症表型和分子途径，使它们成为癌症研究的有力工具。本文综述了从鱼类到人类癌症研究的转变，重点介绍了鱼类癌症的分子基础、诊断和治疗。主要发现包括血管形成在鱼类肿瘤中的作用，以及应用斑马鱼肿瘤模型来了解人类癌症。这些研究为人类癌症研究提供了新的视角和技术，尽管在充分利用鱼类模型促进未来治疗进展方面仍存在挑战。

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The plastic threat to marine gastropods: Oxidative stress, energy metabolism and impaired recovery in juvenile abalone (Haliotis discus hannai) exposed to polystyrene microplastics 塑料对海洋腹足类动物的威胁：暴露于聚苯乙烯微塑料的幼鲍鱼（Haliotis discus hannai）的氧化应激、能量代谢和恢复受损

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2026-01-01 Epub Date: 2025-09-19 DOI: 10.1016/j.cbpc.2025.110361

Chaoyi Xie , Hongce Song , Junjie Li , Shuai Xu , Liqun Ren , Chunnuan Zhao , Yuping Wu , Xiaotong Wang , Biao Wu , Shubao Zhang , Haiying Han , Tao Yu

Microplastic (MP) pollution challenges marine ecosystems, with gastropods like Haliotis discus hannai being potentially vulnerable. However, the reversibility of MP-induced physiological damage in gastropods remains poorly understood. This study investigated the effects of polystyrene microplastics (PS-MPs; 0, 100, and 1000 μg/L) on H. discus hannai in a 14-day exposure followed by a 7-day recovery experimental design. The analysis focused on antioxidant status, energy metabolism, and immune pathways. Results showed dose-dependent oxidative stress during PS-MPs exposure, indicated by significantly (P < 0.05) elevated antioxidant enzyme activities (Superoxide dismutase, Catalase and Glutathione peroxidase), which decreased but did not normalize during recovery. Exposure to a low concentration of PS-MPs (100 μg/L) resulted in metabolic adjustments consistent with homeostatic maintenance, whereas a high concentration (1000 μg/L) significantly increased lactate dehydrogenase and Na⁺/K⁺-ATPase activities, suggesting metabolic disruption. These energy metabolism alterations persisted, showing incomplete recovery. Immune pathway analysis revealed minimal changes at low PS-MPs concentrations but significant enrichment of pathways like IL-17 and Fc-Epsilon-RI signaling at high concentrations during exposure. Notably, sustained activation of immune pathways, including NOD-like receptor, Toll-like receptor, and IL-17 signaling (particularly in the high-concentration group), persisted during recovery, potentially indicating prolonged cellular stress or links to apoptosis. Furthermore, Integrated Biomarker Response (IBR) analysis confirmed that H. discus hannai remained severely impacted even after the recovery period. This study provides crucial evidence on the complex toxicodynamics of PS-MPs in H. discus hannai, highlighting incomplete physiological recovery and significant damage during the recovery period following exposure, especially at high concentrations.

微塑料污染对海洋生态系统构成了挑战，像盘藻这样的腹足类动物可能很脆弱。然而，mp诱导的腹足类生理损伤的可逆性仍然知之甚少。本研究研究了聚苯乙烯微塑料（PS-MPs； 0、100和1000 μg/L）在14天的暴露下对虹桃的影响，然后进行了7天的恢复实验设计。分析的重点是抗氧化状态、能量代谢和免疫途径。结果显示，PS-MPs暴露期间存在剂量依赖性氧化应激，表现为抗氧化酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）活性显著（P < 0.05）升高，恢复期间活性下降但未恢复正常。暴露于低浓度PS-MPs （100 μg/L）下导致代谢调节符合稳态维持，而高浓度PS-MPs （1000 μg/L）显著增加乳酸脱氢酶和Na+/K+- atp酶活性，提示代谢紊乱。这些能量代谢改变持续存在，表现为不完全恢复。免疫途径分析显示，低PS-MPs浓度下的变化很小，但高浓度暴露时IL-17和Fc-Epsilon-RI信号通路显著富集。值得注意的是，免疫通路的持续激活，包括nod样受体、toll样受体和IL-17信号传导（特别是在高浓度组），在恢复过程中持续存在，可能表明细胞应激延长或与凋亡有关。此外，综合生物标志物反应（IBR）分析证实，即使在恢复期后，铁饼猴仍然受到严重影响。本研究提供了重要的证据，证明了pps - mps在铁饼鱼体内的复杂毒理学，强调了暴露后的不完全生理恢复和恢复期的显著损害，特别是在高浓度下。

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