Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献_第6页

Rare earth element lanthanum induces inflammatory response in zebrafish through TLR4/NF-κB signaling pathway 稀土元素镧通过TLR4/NF-κB信号通路诱导斑马鱼炎症反应。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-20 DOI: 10.1016/j.cbpc.2025.110391

Xinhao Ye , Shiyi Duan , Hao Wang , Shimei Xiao , Mijia Li , Wei Yuan , Yan Zhao , Yiyue Zhang , Keyuan Zhong

The inflammatory response is a core protective physiological process against stimuli like infection or injury, and can be initiated by autoimmune disorders. It is primarily characterized by neutrophil-dominated leukocytosis and may lead to multiple organ dysfunction in severe cases. Environmental factors play an important role in the inflammatory response. Rare earth elements are not essential elements for living organisms. However, owing to large-scale mining and use, their concentrations in the environment have increased. Thus, rare earth elements are now considered emerging environmental pollutants, and the risks that rare earth elements pose to human health need further investigation. In this study, zebrafish were used as experimental animals, and zebrafish embryos were exposed to the different concentrations of lanthanum chloride (0, 5, 15, and 25 mg/L) to analyze its effect on embryo development and immune system. The number and distribution of zebrafish neutrophils as well as changes in oxidative stress and the expression of genes related to inflammation were analyzed. The results indicated that lanthanum chloride exposure reduced the heart rate, shortened the body length, and increased the yolk area of zebrafish embryos. In addition, exposure to lanthanum chloride caused the diffusion of neutrophils, leading to inflammation in zebrafish. Concurrently, the exposure led to the accumulation of reactive oxygen species in zebrafish, which subsequently resulted in the upregulation of malondialdehyde, catalase, and superoxide dismutase levels. Further experiments revealed that exposure to lanthanum chloride led to the upregulation of several inflammation-related genes, such as il-6, il-8, il-10, and cxcl-c1c, as well as certain TLR4/NF-κB signaling-related genes, including tlr4, myd88, nf-κb p65, il-1β, and tnf-α. The TLR4/NF-κB signaling pathway inhibitor andrographolide can alleviate the inflammatory response induced by lanthanum chloride exposure. In conclusion, lanthanum chloride induced inflammation in zebrafish by activating the TLR4/NF-κB signaling pathway. The study results can provide a reference for evaluating the health risks of rare earth elements in humans.

炎症反应是针对感染或损伤等刺激的核心保护性生理过程，可由自身免疫性疾病引发。它主要以中性粒细胞为主的白细胞增多为特征，严重者可导致多器官功能障碍。环境因素在炎症反应中起重要作用。稀土元素不是生物体的必需元素。但是，由于大规模开采和使用，它们在环境中的浓度有所增加。因此，稀土元素现在被认为是新兴的环境污染物，稀土元素对人类健康构成的风险需要进一步调查。本研究以斑马鱼为实验动物，将斑马鱼胚胎暴露于不同浓度的氯化镧（0、5、15和25 mg/L）中，分析其对胚胎发育和免疫系统的影响。分析斑马鱼中性粒细胞的数量和分布，以及氧化应激和炎症相关基因的表达变化。结果表明，氯化镧使斑马鱼胚胎心率降低，体长缩短，卵黄面积增大。此外，暴露于氯化镧引起中性粒细胞的扩散，导致斑马鱼炎症。同时，暴露导致斑马鱼体内活性氧积累，进而导致丙二醛、过氧化氢酶和超氧化物歧化酶水平上调。进一步的实验表明，暴露于氯化镧会导致几种炎症相关基因，如IL-6、IL-8、IL-10和cxcl-c1c，以及某些TLR4/NF-κB信号相关基因，包括TLR4、MyD88、NF-κB P65、IL-1β和TNF-α的上调。TLR4/NF-κB信号通路抑制剂穿心莲内酯可减轻氯化镧暴露引起的炎症反应。综上所述，氯化镧通过激活TLR4/NF-κB信号通路诱导斑马鱼炎症。研究结果可为评价稀土元素对人体的健康风险提供参考。

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引用次数: 0

K channels and action potential in dorsal root ganglion of diverse animals 不同动物背根神经节的K通道和动作电位。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-20 DOI: 10.1016/j.cbpc.2025.110398

Sodikdjon A. Kodirov

The phenotype of action potentials (AP) in mammalian dorsal root ganglion (DRG) neurons is biphasic and thereby distinct from those in the CNS and spinal cord. The sensation of pain by DRG and its prevention may occur via many types of channels, receptors, and neurotransmitters; these are at least Cav, Kv, Nav, and TRP. The Cav, Kv, and Nav channels are prevailingly involved in the excitability of DRG neurons, while the TRP family enables the mechanosensitivity. The latter are the main family of channels, and thereby the list is extensive because of the presence of many distinct α subunits among them. Also, all major receptor channels are described in DRG, but purinergic ones could be considered important because of sensitization to ATP as a neurotransmitter. This work presents a comparative and detailed synthesis of the electrophysiological properties of intact DRG and isolated neurons, with an emphasis on the K channels involved in action potential generation.

哺乳动物背根神经节（DRG）神经元的动作电位（AP）表型是双相的，因此与中枢神经系统和脊髓中的动作电位不同。DRG对疼痛的感觉及其预防可能通过多种通道、受体和神经递质发生；这些至少是Cav， Kv， Nav和TRP。Cav、Kv和Nav通道主要参与DRG神经元的兴奋性，而TRP家族则参与机械敏感性。后者是主要的通道族，由于其中存在许多不同的α亚基，因此该列表很广泛。此外，DRG中描述了所有主要的受体通道，但嘌呤能通道可能被认为是重要的，因为它对ATP作为一种神经递质敏感。本研究对完整DRG和分离神经元的电生理特性进行了比较和详细的综合，重点研究了参与动作电位产生的K通道。

引用次数: 0

Phenol exposure promotes tumor-related signaling and blood vessel formation through the extracellular signal-regulated kinase/p38/hypoxia-inducible factor-1α pathway in cellular and zebrafish models 在细胞和斑马鱼模型中，苯酚暴露通过细胞外信号调节激酶/p38/缺氧诱导因子-1α途径促进肿瘤相关信号传导和血管形成。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-19 DOI: 10.1016/j.cbpc.2025.110396

Chung-Yu Lin , Wangta Liu , Pei-Hsuan Chen , Chia C. Wang , Che-Hsin Lee

Phenol is a common aquatic contaminant originating from industrial discharge, plastics, and personal care products, and is frequently detected due to its high solubility and environmental persistence. Although its acute toxicity is well documented, the effects of phenol at environmentally relevant concentrations on cellular mechanisms linked to tumor progression remain underexplored. In this study, we investigated the impact of phenol exposure (0–125 μM) on cancer-related cellular behaviors using B16F10 melanoma and LL2 lung carcinoma cells, as well as zebrafish xenograft models, which serve as an integrated aquatic toxicology platform. Phenol exposure activated extracellular signal-regulated kinase (ERK) and p38 pathways, upregulated hypoxia-inducible factor 1α (HIF-1α), increased vascular endothelial growth factor (VEGF) expression, and induced epithelial–mesenchymal transition (EMT). These molecular events collectively enhanced tumor cell migration and angiogenesis both in vitro and in vivo. Our findings provide mechanistic evidence that environmentally relevant phenol exposure can modulate conserved stress and signaling pathways associated with tumor-related phenotypes. This work underscores the importance of combining molecular biomarkers with aquatic vertebrate models to assess the ecological and toxicological risks of persistent organic pollutants such as phenol.

苯酚是一种常见的水生污染物，源于工业排放、塑料和个人护理产品，由于其高溶解度和环境持久性，经常被检测到。尽管其急性毒性已被充分证明，但环境相关浓度下苯酚对肿瘤进展相关细胞机制的影响仍未得到充分研究。在这项研究中，我们研究了苯酚暴露（0-125 μM）对癌症相关细胞行为的影响，使用B16F10黑色素瘤和LL2肺癌细胞以及斑马鱼异种移植模型，作为综合水生毒理学平台。苯酚暴露激活细胞外信号调节激酶（ERK）和p38通路，上调缺氧诱导因子1α (HIF-1α)，增加血管内皮生长因子（VEGF）表达，诱导上皮-间质转化（EMT）。这些分子事件共同增强了肿瘤细胞在体内和体外的迁移和血管生成。我们的研究结果提供了机制证据，表明环境相关的苯酚暴露可以调节与肿瘤相关表型相关的保守应激和信号通路。这项工作强调了将分子生物标志物与水生脊椎动物模型结合起来评估持久性有机污染物（如苯酚）的生态和毒理学风险的重要性。

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引用次数: 0

Polystyrene microplastics and nanoplastics induce neurotoxicity in zebrafish via oxidative stress and neurotransmitter disruption 聚苯乙烯微塑料和纳米塑料通过氧化应激和神经递质破坏诱导斑马鱼神经毒性。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-19 DOI: 10.1016/j.cbpc.2025.110397

Jiejie Li , Yingjie Chen , Yuequn Chen , Han Xie , Ganglong Wu , Yiming Zhang , Kusheng Wu

The widespread use of plastic products has led to the global accumulation of microplastics (MPs) and nanoplastics (NPs) in aquatic and terrestrial environments, posing significant risks to ecosystems and human health. This study investigated the neurodevelopmental toxicity of polystyrene MPs (PS-MPs, 5 μm) and NPs (PS-NPs, 60 nm) in zebrafish (Danio rerio) and explored the underlying mechanisms. Zebrafish embryos were exposed to 0.05–50 mg/L PS-MPs/PS-NPs from 2 hour post-fertilization (hpf) to 7 days post-fertilization (dpf). Morphological, behavioral, and molecular endpoints were analyzed. Exposure to polystyrene MPs and NPs (PS-MNPs) induced dose-dependent developmental malformations, including spinal curvature, pericardial edema, and abnormal body pigmentation, accompanied by increased heart rate and body length. Behavioral assays revealed reduced spontaneous tail-coiling in embryos and hyperactive swimming in larvae, particularly under light stimulation. Mechanistic studies showed PS-MNPs disrupted neurotransmitter homeostasis (reduced dopamine, acetylcholine, GABA, and serotonin levels) and altered neurodevelopment-related gene expression (e.g., mbpa, ache, gfap). Oxidative stress was evident via elevated reactive oxygen species (ROS) and upregulated antioxidant genes (sod1, cat) in PS-NP-exposed larvae. These findings demonstrate that PS-MNPs induce neurodevelopmental toxicity in zebrafish through oxidative stress and neurotransmitter system dysfunction, highlighting the potential risks of plastic pollution to aquatic organisms and human health via trophic transfer.

塑料产品的广泛使用导致全球水生和陆地环境中微塑料（MPs）和纳米塑料（NPs）的积累，对生态系统和人类健康构成重大风险。本研究研究了聚苯乙烯MPs （PS-MPs, 5 μm）和NPs （PS-NPs, 60 nm）对斑马鱼的神经发育毒性，并探讨了其机制。斑马鱼胚胎在受精后2 小时（hpf）至7 天（dpf）暴露于0.05-50 mg/L的PS-MPs/PS-NPs中。形态学、行为学和分子终点分析。暴露于聚苯乙烯MPs和NPs （PS-MNPs）会引起剂量依赖性发育畸形，包括脊柱弯曲、心包水肿和异常的身体色素沉着，并伴有心率和体长增加。行为分析显示，在光刺激下，胚胎自发尾卷减少，幼虫过度活跃游泳。机制研究表明，PS-MNPs破坏神经递质稳态（降低多巴胺、乙酰胆碱、GABA和血清素水平），并改变神经发育相关基因表达（如mbpa、ache、gfap）。在ps - np暴露的幼虫中，氧化应激通过活性氧（ROS）的升高和抗氧化基因（sod1, cat）的上调而明显。这些研究结果表明，PS-MNPs通过氧化应激和神经递质系统功能障碍诱导斑马鱼神经发育毒性，突出了塑料污染通过营养转移对水生生物和人类健康的潜在风险。

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引用次数: 0

Developmental hepatotoxicity induced by flusilazole in zebrafish: Mechanistic insights into mitochondrial dysfunction, oxidative stress, ferroptosis, and regenerative impairment 氟美唑诱导斑马鱼发育性肝毒性：线粒体功能障碍、氧化应激、铁下垂和再生损伤的机制

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-19 DOI: 10.1016/j.cbpc.2025.110393

Hojun Lee , Jisoo Song , Garam An , Seung-Min Bae , Gwonhwa Song , Whasun Lim , Sunwoo Park

Flusilazole is a triazole-based fungicide that persists in various environments because of its high stability and solubility, raising concerns about its developmental and ecological impacts. Although numerous studies have reported flusilazole-induced toxicity, the specific effects and mechanisms of flusilazole-induced hepatotoxicity during development remain unclear. In this study, we examined the in vivo and in vitro toxicities in Danio rerio (zebrafish) and zebrafish-derived liver (ZFL) cells. Morphological changes in the liver and alterations in liver regeneration were evaluated using fabp10a:dsRed and fabp10a:CFP-NTR transgenic models. Flusilazole exposure was shown to deteriorate hepatic structure and regenerative capacity, with potential long-term consequences for aquatic organisms. Moreover, in ZFL cells, flusilazole treatment induced oxidative stress, mitochondrial malfunction, and disruption of calcium and iron homeostasis, leading to the induction of apoptosis and ferroptosis. Transcriptomic analysis supported these findings. Additionally, disturbances in ERK and Akt signaling indicated interference with pathways central to cell survival, growth, and tissue repair. Together, these findings establish that flusilazole exerts developmental hepatotoxic effects and highlight its potential hazards to ecosystems.

氟咪唑是一种基于三唑的杀菌剂，由于其高稳定性和溶解度，在各种环境中持续存在，引起了人们对其发育和生态影响的关注。尽管许多研究报道了氟拉唑诱导的毒性，但在发育过程中，氟拉唑诱导的肝毒性的具体作用和机制尚不清楚。在这项研究中，我们检测了在斑马鱼和斑马鱼源性肝脏（ZFL）细胞中的体内和体外毒性。采用fabp10a:dsRed和fabp10a:CFP-NTR转基因模型评估肝脏形态学变化和肝脏再生的改变。氟西拉唑暴露被证明会使肝脏结构和再生能力恶化，对水生生物具有潜在的长期后果。此外，在ZFL细胞中，氟唑唑处理诱导氧化应激、线粒体功能障碍、钙和铁稳态破坏，导致细胞凋亡和铁凋亡。转录组学分析支持了这些发现。此外，ERK和Akt信号的干扰表明细胞存活、生长和组织修复的核心途径受到干扰。综上所述，这些发现表明氟美唑具有发育性肝毒性作用，并强调了其对生态系统的潜在危害。

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引用次数: 0

Astaxanthin mitigates the inflammatory toxicity of microcystin-LR on zebrafish embryos 虾青素减轻微囊藻毒素lr对斑马鱼胚胎的炎症毒性。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-19 DOI: 10.1016/j.cbpc.2025.110399

Yahui Wu , Qing Huang

Astaxanthin (AST) as a natural carotenoid exhibits potent antioxidant and anti-inflammatory capacities. This work investigated AST’s protective effects against microcystin-LR (MC-LR) toxicity to zebrafish embryos. When the zebrafish embryos were exposed to a sublethal, environmentally relevant concentration of MC-LR (10 μg/L, approximately half of the LC₅₀ value), AST (100 μg/L) could significantly reduce MC-LR-induced mortality by 39.8% and deformity rates by 60.0%. Furthermore, AST decreased ROS and MDA levels by 11.0% and 14.5%, respectively, and enhanced the activities of superoxide dismutase (SOD, 4.4-fold), catalase (CAT, 1.2-fold), and glutathione reductase (GR, 1.6-fold). It also ameliorated MC-LR-induced inflammatory responses, as evidenced by a 49.1% reduction in neutral red staining, a 42.0% to 42.9% improvement in host resistance, and a significant down-regulation of major cytokines (IL-1β, IL-6, IL-8, TNF-α) by 0.4 to 0.6-fold. Analysis of the transcriptome revealed that AST can inhibit the C-type lectin receptor signaling pathway and others to counteract the inflammatory and oxidative stress induced by MC-LR. Our findings confirm that AST neutralizes the toxicity of MC-LR through the mechanisms of antagonizing oxidative stress, exhibiting anti-inflammatory and immunomodulatory effects, which may pave the way for AST being used in aquaculture and environmental health.

虾青素（AST）作为一种天然类胡萝卜素，具有很强的抗氧化和抗炎能力。本研究探讨了AST对斑马鱼胚胎微囊藻毒素（MC-LR）毒性的保护作用。当斑马鱼胚胎暴露于亚致死的环境相关浓度的MC-LR（10 μg/L，约为LC50值的一半）时，AST（100 μg/L）可以显著降低MC-LR诱导的死亡率39.8% %和畸形率60.0 %。此外，AST降低ROS和MDA水平分别为11.0 %和14.5 %，提高超氧化物歧化酶（SOD， 4.4倍）、过氧化氢酶（CAT， 1.2倍）和谷胱甘肽还原酶（GR， 1.6倍）的活性。它还改善了mc - lr诱导的炎症反应，证明中性红染色降低49.1% %，宿主抗性提高42.0% %至42.9% %，主要细胞因子（IL-1β, IL-6, IL-8, TNF-α）显著下调0.4至0.6倍。转录组分析显示，AST可以抑制c型凝集素受体信号通路等，对抗MC-LR诱导的炎症和氧化应激。我们的研究结果证实，AST通过拮抗氧化应激机制中和MC-LR的毒性，具有抗炎和免疫调节作用，这可能为AST在水产养殖和环境健康中的应用铺平道路。

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引用次数: 0

Concurrent endoplasmic reticulum stress and demyelination in DEHP-exposed zebrafish larvae at the early developmental stages 暴露于dehp的斑马鱼幼体在早期发育阶段并发内质网应激和脱髓鞘。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-19 DOI: 10.1016/j.cbpc.2025.110394

Garima Jindal , Anuradha Mangla , Mehjbeen Javed , Mohd. Anas Saifi , Iqra Mazahir , Padmshree Mudgal , Shiekh Raisuddin

Di-ethylhexyl phthalate (DEHP) is an endocrine disruptor with established neurotoxic as well as potential neurodegenerative effects. The myelin sheath plays a crucial role in maintaining the health of the nervous system, whereas demyelination contributes to the onset of brain diseases. This study investigated the effect of DEHP on the neurological development with special reference to endoplasmic reticulum (ER) stress, inflammation, and concurrently with demyelination and cellular apoptotic development in zebrafish larvae. Results indicated that DEHP exposure can lead to demyelination through ER stress and inflammation, as evident from the decreased expression of myelin basic protein (Mbp) in both the brain and spinal cord of zebrafish larvae analyzed through immunofluorescent assay. The mRNA expression of axon marker nfl significantly increased, while tuba1a was decreased with DEHP exposure. Western blotting analysis revealed that ER stress markers such as phosphorylated inositol-requiring enzyme 1 alpha (p-Ire1α), activating transcription factor 4 (Atf4), binding immunoglobulin protein (Bip), phosphorylated e-IF2 alpha (p-eIF2α), CCAAT/enhancer-binding protein homologous protein (Chop), and inflammatory markers (nuclear factor kappa B subunit p65; Nf-κb p65), ionized calcium-binding adaptor molecule 1 (Iba1), and glial fibrillary acid protein (Gfap), were significantly upregulated on exposure to DEHP. Scototaxis, a behavioral assay, showed an altered anxiety-like behaviour in DEHP-treated larvae. Oxidative stress markers, such as superoxide dismutase (SOD), catalase, and monoamine oxidase (MAO) were also elevated. Apoptotic cells were observed in DEHP-treated zebrafish larvae in acridine orange staining. Overall, the DEHP exposure to zebrafish larvae caused myelin sheath degeneration and axonal dysfunction due to the generation of ER stress and inflammation.

邻苯二甲酸二乙基己酯（DEHP）是一种内分泌干扰物，具有神经毒性和潜在的神经退行性作用。髓鞘在维持神经系统健康方面起着至关重要的作用，而脱髓鞘则会导致脑部疾病的发生。本研究探讨了DEHP对斑马鱼幼体神经发育的影响，特别是内质网应激、炎症以及同时发生的脱髓鞘和细胞凋亡发育。结果表明，DEHP暴露可通过内质网应激和炎症导致脱髓鞘脱髓鞘，免疫荧光分析显示斑马鱼幼鱼脑和脊髓中髓鞘碱性蛋白（Mbp）表达降低。DEHP暴露后，轴突标记物nfl mRNA表达显著升高，tuba1a mRNA表达降低。内质网应激标志物如肌醇要求酶1 α (Ire1)、激活转录因子4 （Atf4）、结合免疫球蛋白蛋白（Bip）、磷酸化e-IF2 α （p-eIF2α)、CCAAT/增强子结合蛋白同源蛋白（Chop）、炎症标志物（核因子κ B亚基p65； Nf-κb p65）、离子钙结合接头分子1 （Iba1）和胶质原纤维酸蛋白（Gfap））在DEHP暴露时显著上调。在dehp处理的幼虫中，行为性测定也发生了变化。氧化应激标志物如超氧化物歧化酶（SOD）、过氧化氢酶和单胺氧化酶（MAO）也升高。用吖啶橙染色观察dehp处理的斑马鱼幼虫细胞凋亡。总体而言，DEHP暴露于斑马鱼幼虫后，由于内质网应激和炎症的产生，导致髓鞘变性和轴突功能障碍。

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引用次数: 0

Non-lethal heat shock induces cross-tolerance to different stressors in two strains of Brachionus koreanus (Rotifera: Monogononta): Mechanisms of increased tolerance to hydrogen peroxide 非致死性热休克诱导两株朝鲜臂尾虫对不同应激源的交叉耐受性：过氧化氢耐受性增加的机制。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-14 DOI: 10.1016/j.cbpc.2025.110389

Luana Granada , Inês F.C. Morão , Marco F.L. Lemos , Peter Bossier , Sara C. Novais

The rotifer Brachionus sp. is of great importance for aquaculture, as the reproduction cycle under rearing conditions of many economically important species larvae depends on the use of rotifers as first live feed. Establishing a protocol that results in an improved tolerance of rotifers to environmental stressors will allow for a more stable rotifer production. The exposure to non-lethal heat shocks (NLHS) already proved to enhance the tolerance, not only to heat stress, but also to other stressors in several aquatic species, by activating the heat shock response and epigenetic mechanisms. This study aimed to determine the potential of a single NLHS to induce tolerance to different abiotic stressors in two strains of B. koreanus (MRS10 and IBA3) and to evaluate possible molecular mechanisms involved in the achievement of increased tolerance to hydrogen peroxide induced by NLHS. Cross-tolerance was achieved for both strains, namely to high salinity, cadmium chloride, and hydrogen peroxide. Scale-up tests resulted in increased tolerance to hydrogen peroxide only for MRS10. During the exposure to this substance, heat-shocked MRS10 rotifers showed an up-regulation of genes related to oxidative stress response and histone modifications, increased production of HSP70, and higher levels of total acetylation of histone H3. A single NLHS proved to induce epigenetic effects when rotifers were exposed to other stressor later in life. However, further studies should elucidate if the NLHS conditions used in this study can yield a persistent outcome, allowing the establishment of tolerant rotifer strain lines and, consequently, a more stable production.

轮虫Brachionus sp.对水产养殖具有重要意义，因为许多经济上重要的物种幼虫在饲养条件下的繁殖周期依赖于将轮虫作为第一活饲料。建立一种方案，提高轮虫对环境压力的耐受性，将使轮虫的生产更加稳定。暴露于非致死性热冲击（NLHS）已经被证明不仅可以通过激活热冲击反应和表观遗传机制来增强对热应激的耐受性，而且还可以通过激活热冲击反应和其他应激源来增强对几种水生物种的耐受性。本研究旨在确定单一NLHS诱导两株韩国双歧杆菌（MRS10和IBA3）对不同非生物应激源耐受性的潜力，并评估NLHS诱导过氧化氢耐受性增强的可能分子机制。这两种菌株对高盐、氯化镉和过氧化氢具有交叉耐受性。扩大试验结果表明，只有MRS10对过氧化氢的耐受性增加。在暴露于这种物质期间，热休克MRS10轮虫表现出与氧化应激反应和组蛋白修饰相关的基因上调，HSP70的产生增加，组蛋白H3的总乙酰化水平更高。当轮虫在以后的生活中暴露于其他压力源时，单个NLHS被证明会诱导表观遗传效应。然而，进一步的研究应该阐明本研究中使用的NLHS条件是否能产生持久的结果，从而建立耐受性轮虫品系，从而实现更稳定的生产。

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引用次数: 0

Ancestral exposure to amitriptyline disrupts the behavior and gene expression in zebrafish F2 offspring 祖先接触阿米替林会破坏斑马鱼F2后代的行为和基因表达。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-13 DOI: 10.1016/j.cbpc.2025.110390

Anqi Liu , Kun Chen , Xuchun Qiu , Yuki Takai , Yohei Shimasaki , Yuji Oshima

Amitriptyline (AMI), a commonly used tricyclic antidepressant, has been identified as a significant pharmaceutical contaminant in aquatic environments. Although parental exposure of zebrafish to AMI has been found to induce changes in the development, behavior, and gene expression of their F1 offspring, it is unclear whether such adverse effects will be further extended to subsequent generations. In the current study, we explored the effects of ancestral exposure to AMI at environmentally relevant concentrations (0 and 0.8 μg/L) on the early life stages of zebrafish F2 offspring. The results showed that ancestral exposure to AMI had no significant effect on the survival and development of the zebrafish F2 offspring. However, significant hyperactivity was observed in the F2 larvae in the ancestral AMI exposure group during the dark periods of a light-dark locomotion assay. Transcription analysis revealed that ancestral exposure to AMI significantly disrupted pathways associated with xenobiotic biodegradation and metabolism, as well as the metabolism of cofactors and vitamins. Furthermore, ancestral exposure to AMI significantly decreased the level of cytochrome P450 and the activity of glutathione S-transferase within the F2 larvae, which are critical enzymes involved in xenobiotic metabolism. These findings provide valuable insights into the multigenerational effects of AMI exposure in zebrafish, emphasizing the importance of assessing the risks posed by such pollutants to fish populations.

阿米替林（AMI）是一种常用的三环抗抑郁药，已被确定为水生环境中的重要药物污染物。虽然已经发现父母接触AMI会诱导F1后代的发育、行为和基因表达发生变化，但尚不清楚这种不良影响是否会进一步延伸到后代。在本研究中，我们探讨了祖先暴露于环境相关浓度（0和0.8 μg/L）的AMI对斑马鱼F2后代早期生命阶段的影响。结果表明，祖先接触AMI对斑马鱼F2后代的生存和发育没有显著影响。然而，在光-暗运动试验的黑暗时期，祖先AMI暴露组的F2幼虫观察到显著的过度活跃。转录分析显示，祖先暴露于AMI显著破坏了与外源生物降解和代谢相关的途径，以及辅助因子和维生素的代谢。此外，祖先暴露于AMI导致F2幼虫体内参与外源代谢的关键酶水平显著降低，特别是细胞色素P450和谷胱甘肽s -转移酶。这些发现为了解AMI暴露对斑马鱼的多代影响提供了有价值的见解，强调了评估此类污染物对鱼类种群构成的风险的重要性。

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引用次数: 0

Anti-sea lice products azamethiphos and hydrogen peroxide effects on five coastal marine organisms 抗海虱产品偶氮硫磷和过氧化氢对五种沿海海洋生物的影响。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-13 DOI: 10.1016/j.cbpc.2025.110392

Davide Asnicar , Benjamin de Jourdan

Azamethiphos and hydrogen peroxide are active ingredients (AI) of formulations used as water-bath pesticides in Atlantic salmon aquaculture to remove ectoparasitic copepods. Despite their long-term use, unknowns and concerns are still present, particularly regarding the toxicity towards non-target commercially and ecologically important species in Atlantic Canada, and potential differences in toxicity between the AI and the formulated product.

Here, we tested the acute effects of azamethiphos and hydrogen peroxide on five marine species. Hazard data (half maximal effective and lethal concentrations, respectively EC50 and LC50) were determined for 10 endpoints, assessed at various timepoints, during the exposure of Stage I larval American lobster Homarus americanus, green sea urchin Strongylocentrotus droebachiensis gametes, haemocytes and adults of blue mussel Mytilus edulis, common periwinkle snail Littorina littorea, and copepod Acartia tonsa.

To investigate whether formulations had the same effect as the AI, for azamethiphos, both the AI and the formulation (Salmosan® Vet) were tested. For hydrogen peroxide, potential differences in the toxicity of three formulations (purchased solutions 50 %, 35 %, and 3 %) were compared.

Results suggest no differences between the azamethiphos and Salmosan® Vet, with similar L/EC50 values. Little differences were found among the three hydrogen peroxide formulations. For azamethiphos, the most sensitive species was the American lobster, followed by sea urchin, whereas for hydrogen peroxide, sea urchin was the most sensitive, followed by A. tonsa. L. littorea was the least sensitive species tested. Overall, results showed that all the endpoints were greater than the Environmental Quality Standard previously determined for the two compounds.

Azamethiphos和过氧化氢是大西洋鲑鱼养殖中用于去除外寄生桡足类动物的水浴农药配方的活性成分。尽管它们长期使用，但未知因素和担忧仍然存在，特别是对加拿大大西洋地区非目标商业和生态重要物种的毒性，以及人工智能和配方产品之间毒性的潜在差异。在这里，我们测试了azamethiphos和过氧化氢对五种海洋物种的急性影响。在不同的时间点，测定了10个终点的危害数据（最大有效浓度和致死浓度的一半，分别为EC50和LC50），评估了暴露于美洲小龙虾（Homarus americanus）、绿海胆（strongylocentrrotus droebacachiensis）配子、蓝贻贝（Mytilus edulis）、常青螺（Littorina littorea）和桡足动物（Acartia tonsa）的I期幼虫。为了研究制剂是否与人工智能具有相同的效果，对噻虫磷进行了人工智能和制剂（Salmosan®Vet）的测试。对于过氧化氢，比较了三种配方（购买的溶液50 %，35 %和3 %）毒性的潜在差异。结果表明，azamethiphos和Salmosan®Vet之间没有差异，L/EC50值相似。三种过氧化氢配方之间差异不大。对偶氮硫磷最敏感的是美洲龙虾，其次是海胆；对双氧水最敏感的是海胆，其次是沙棘。littorea是最不敏感的物种。总体而言，结果表明所有终点都大于先前确定的两种化合物的环境质量标准。

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