Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献_第7页

From metabolites to tissues: A comprehensive analysis of salinity-driven modulation of tetracycline effects in Mytilus galloprovincialis 从代谢物到组织：盐度驱动的四环素在加洛野贻贝中的调节作用的综合分析。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-04 DOI: 10.1016/j.cbpc.2025.110383

Marta Cunha , Alessio Lenzi , Constança Figueiredo , Lucia De Marchi , Carla Leite , Tania Russo , Gianfranca Monni , Valentina Meucci , Amadeu M.V.M. Soares , Gianluca Polese , Eduarda Pereira , Carlo Pretti , Rosa Freitas

Environmental salinity shifts, intensified by climate change, can influence the toxicity of pollutants such as antibiotics in marine organisms. In this study, specimens of the mussel Mytilus galloprovincialis were exposed for 28 days to three salinity levels (20, 30, and 40) in the presence or absence of tetracycline (TC) (1 mg/L). At the end of the exposure period, the effects were evaluated using an integrated metabolomic, biochemical, and histopathological approach. Tetracycline bioaccumulation did not differ significantly across salinities, indicating that biological effects were driven by stressor interactions rather than uptake. Metabolomic profiling showed that salinity and salinity-TC combinations had stronger impacts than TC alone. At salinity 20, mussels exhibited early oxidative stress and metabolic adjustments, along with tissue atrophy and lipofuscin buildup. Mussels at salinity 30 displayed relative physiological stability despite moderate histological changes under TC. In contrast, salinity 40 caused severe cellular damage, including membrane remodeling, lipid peroxidation, depleted antioxidants, and neurotoxic responses. The integrated multi-level analysis revealed coordinated stress responses involving oxidative stress, altered energy metabolism, and detoxification. Overall, these findings highlight salinity 30 as the optimal condition for M. galloprovincialis and emphasize the synergistic effects of climate-driven salinity changes and antibiotic pollution, underscoring the need to account for abiotic stressors in ecotoxicological assessments.

由于气候变化而加剧的环境盐度变化可以影响海洋生物中抗生素等污染物的毒性。在这项研究中，在四环素（1 mg/L）存在或不存在的情况下，将贻贝（Mytilus galloprovincialis）标本暴露在三种盐度水平（20、30和40）下28 天。在暴露期结束时，使用综合代谢组学、生化和组织病理学方法评估其影响。TC的生物积累在不同盐度下没有显著差异，表明生物效应是由应激源相互作用驱动的，而不是由吸收驱动的。代谢组学分析显示，盐度和盐度-TC组合比单独使用TC有更强的影响。盐度为20时，贻贝表现出早期的氧化应激和代谢调节，以及组织萎缩和脂褐素的积累。盐度为30的贻贝在TC下表现出相对的生理稳定性，但组织学变化不大。相反，盐度40引起严重的细胞损伤，包括膜重塑、脂质过氧化、抗氧化剂耗尽和神经毒性反应。综合多层次分析揭示了包括氧化应激、能量代谢改变和解毒在内的协调应激反应。总的来说，这些发现强调了盐度30是加洛省分枝杆菌生存的最佳条件，并强调了气候驱动的盐度变化和抗生素污染的协同效应，强调了在生态毒理学评估中考虑非生物应激源的必要性。

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引用次数: 0

Effects of short-chain chlorinated paraffins on feeding, oxidative status, and multigenerational parameters in the water flea Moina macrocopa 短链氯化石蜡对大水蚤取食、氧化状态和多代参数的影响

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-11-01 DOI: 10.1016/j.cbpc.2025.110384

Jaehee Kim , Seong Duk Do , Jae-Sung Rhee

Despite being persistent pollutants of global concern and frequently detected in aquatic environments, the detrimental effects of short-chain chlorinated paraffins (SCCPs) on aquatic crustaceans remain limited. Here, we analyzed the acute and chronic effects of SCCPs on the freshwater flea Moina macrocopa. The no observed effect concentration (NOEC) value of SCCPs for 48 h was determined to be 0.24 μg L⁻¹, while the 10 % (LC10) and 50 % (LC50) lethality values were measured as 3.7 and 36 μg L⁻¹, respectively. In the acute exposure experiment, exposure to the LC10 value of SCCPs reduced feeding performance, acetylcholinesterase activity, and thoracic limb movement. In response to the LC10 value, reactive oxygen species levels increased, accompanied by elevated concentrations of malondialdehyde and glutathione. Enzymatic activities of glutathione S-transferase, catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase were significantly elevated at the LC10 value, indicating active involvement of the antioxidant defense system in mitigating oxidative stress. Long-term exposure to the 1/10 NOEC and NOEC values across three generations showed multigenerational detrimental impacts of SCCPs, including reductions in survival, growth, and reproduction in the second and/or third generations. Taken together, our results suggest that even sublethal concentrations of SCCPs can acutely induce cholinergic impairment and oxidative stress, while chronically impairing population maintenance in M. macrocopa.

尽管短链氯化石蜡（SCCPs）是全球关注的持久性污染物，并且经常在水生环境中被检测到，但对水生甲壳类动物的有害影响仍然有限。在此，我们分析了sccp对淡水蚤Moina macrocopa的急性和慢性影响。测定48 h SCCPs的无效应浓度（NOEC）值为0.24 μ L-1, 10 % （LC10）和50 % （LC50）致死值分别为3.7和36 μ L-1。在急性暴露实验中，暴露于LC10值的SCCPs降低了摄食性能、乙酰胆碱酯酶活性和胸部肢体运动。随着LC10值的升高，活性氧水平升高，丙二醛和谷胱甘肽浓度升高。在LC10值时，谷胱甘肽s -转移酶、过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性显著升高，表明抗氧化防御系统积极参与缓解氧化应激。长期暴露在1/10的NOEC和NOEC值下的三代显示出sccp的多代有害影响，包括第二代和/或第三代的生存、生长和繁殖减少。综上所述，我们的研究结果表明，即使是亚致死浓度的SCCPs也能急性诱导胆碱能损伤和氧化应激，同时慢性损害巨巨支原体的种群维持。

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引用次数: 0

Triclosan induces spermatogenic damage via the oxidative stress-P53-apoptosis pathway in zebrafish 三氯生通过氧化应激- p53 -凋亡通路诱导斑马鱼生精损伤。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-31 DOI: 10.1016/j.cbpc.2025.110379

Fan Wang, Yiran Liu, Huiwen Yang, Ying Zhang, Fei Liu

Triclosan (TCS), a widely used antimicrobial agent, has been identified as a reproductive endocrine disruptor. However, the mechanisms underlying TCS-induced gonadal damage remain incompletely understood. In this study, zebrafish were chronically exposed to TCS from 4 h post-fertilization (hpf) to 120 days post-fertilization (dpf). A comprehensive analysis was conducted, including assessment of testicular cell apoptosis in adult fish, evaluation of apoptosis-related genes and proteins in both adult testes and 50-day-old juveniles, and transcriptome sequencing. The results showed that TCS exposure downregulated mRNA expression of oxidative stress-related and anti-apoptotic genes, while upregulating pro-apoptotic gene expression along with P53 and Caspase 3 protein levels, ultimately leading to a significant increase in testicular apoptotic cells. Transcriptome sequencing analysis revealed enrichment of the P53 signaling pathway, apoptosis pathway, response to oxidative stress biological process. These findings indicate that postembryonic exposure to TCS causes gonadal damage primarily by inducing oxidative stress, which increases P53 expression, subsequently regulating pro-apoptotic gene expression and suppressing anti-apoptotic proteins, thereby activating the mitochondrial apoptosis pathway and death receptor pathway, potentially affecting growth, development, and reproductive toxicity. This study systematically elucidates the molecular mechanism by which TCS induces reproductive toxicity through the oxidative stress-P53 axis, which triggers both the mitochondrial and death receptor apoptotic pathways. Our findings provide important experimental evidence and a theoretical reference for the scientific assessment of TCS-related reproductive health risks.

三氯生（TCS）是一种广泛使用的抗菌剂，已被确定为生殖内分泌干扰物。然而，tcs诱导性腺损伤的机制仍不完全清楚。在本研究中，斑马鱼从受精后4 h （hpf）到受精后120 d （dpf）长期暴露于TCS。我们进行了全面的分析，包括评估成鱼睾丸细胞凋亡，评估成鱼睾丸和50日龄幼鱼中凋亡相关基因和蛋白，以及转录组测序。结果表明，TCS暴露可下调氧化应激相关基因和抗凋亡基因的mRNA表达，上调促凋亡基因的表达以及P53和Caspase 3蛋白水平，最终导致睾丸凋亡细胞显著增加。转录组测序分析显示P53信号通路、凋亡通路富集，响应氧化应激的生物过程。这些研究结果表明，胚胎后暴露于TCS主要通过诱导氧化应激导致性腺损伤，氧化应激增加P53表达，随后调节促凋亡基因表达，抑制抗凋亡蛋白，从而激活线粒体凋亡途径和死亡受体途径，可能影响生长发育和生殖毒性。本研究系统阐明了TCS通过氧化应激- p53轴诱导生殖毒性的分子机制，该轴触发线粒体和死亡受体凋亡途径。本研究结果为科学评估tcs相关生殖健康风险提供了重要的实验依据和理论参考。

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引用次数: 0

Behavioral and biochemical effects of benzophenone-3 ingestion in dusky damselfish Stegastes fuscus 食入二苯甲酮-3对暗鲷的行为和生化影响。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-31 DOI: 10.1016/j.cbpc.2025.110386

Jéssica Ferreira de Souza , Mayara Moura Silveira , Ana Luisa Pires Moreira , Juliana Alves Costa Ribeiro Souza , Rafael Xavier Martins , Davi Farias , Francisco Carlos da Silva Junior , Ana Carolina Luchiari

Oxybenzone (also known as benzophenone-3 or BP-3) is an organic ultraviolet (UV) filter commonly used in personal care products. BP-3 has been detected in various aquatic environments and is a major concern in reef areas due to their biological richness and vital role in marine ecosystems. This research focused on investigating the effects of BP-3 exposure in dusky damselfish Stegastes fuscus, an endemic species of the Brazilian coast, analyzing behavioral responses, enzymatic biomarkers on encephalon and liver (catalase (CAT), glutathione S-transferase (GST), acetylcholinesterase (AChE), and lactate dehydrogenase (LDH)), and general health indicators (growth rate and hepatosomatic index). Adults of S. fuscus were fed a diet containing BP-3 at concentrations of 10 μg/g food and 20 μg/g food for 44 days, with behavioral tests starting after 30 days of exposure. Light-dark preference, novel tank and aggressiveness tests were conducted. Our results showed that BP-3 exposure decreased health indicators and altered fish behavior, decreasing risk-perception and locomotion, although agonistic behavior remained unaffected. Enzymatic assays revealed changes that varied depending on the tissue analyzed. These findings highlight the potential of BP-3 to impair behavioral and physiological processes in reef fish, emphasizing the need for regulations on UV filters to protect marine ecosystems and reef life.

氧苯酮（也称为二苯甲酮-3或BP-3）是一种有机紫外线（UV）过滤器，通常用于个人护理产品。BP-3已在各种水生环境中被检测到，由于其丰富的生物多样性和在海洋生态系统中的重要作用，它是珊瑚礁地区的主要关注点。本研究旨在研究BP-3暴露对巴西海岸特有物种褐豆鲷的影响，分析其行为反应、脑和肝脏酶生物标志物（过氧化氢酶（CAT）、谷胱甘肽s -转移酶（GST）、乙酰胆碱酯酶（AChE）和乳酸脱氢酶（LDH））以及一般健康指标（生长速度和肝体指数）。以10 μg和20 μg/g的BP-3为饵料饲喂褐曲虫成虫44 天，暴露30 天后开始行为测试。进行了光暗偏好、新槽和侵袭性试验。我们的研究结果表明，BP-3暴露降低了鱼的健康指标，改变了鱼的行为，降低了风险感知和运动，尽管激动行为不受影响。酶分析揭示了不同组织的变化。这些发现强调了BP-3对珊瑚鱼行为和生理过程的潜在影响，强调了对紫外线过滤器进行监管以保护海洋生态系统和珊瑚礁生物的必要性。

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引用次数: 0

Effects of environmental factors on host-microbiota interactions in the guts of aquatic organisms: A review 环境因素对水生生物肠道宿主-微生物相互作用的影响

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-31 DOI: 10.1016/j.cbpc.2025.110381

Deok-Seo Yoon , Jin-Hyoung Kim , Il-Chan Kim , Youji Wang , Zhou Yang , Min-Chul Lee , Jae-Seong Lee

Aquatic environments are dynamic systems where multiple factors influence the intricate interactions between hosts and their gut microbiomes. This review explores how various stressors alter the gut microbiota of fish and aquatic invertebrates, by examining factors that include water characteristics, photoperiod, external pollutants such as heavy metals and microplastics, food availability, and practical aquaculture feed additives, for example, ethoxyquin. Across these diverse factors, common patterns emerge, including disruptions to microbial diversity, compromised gut barrier integrity, and the induction of oxidative stress. Conversely, beneficial additives like probiotics and astaxanthin are shown to mitigate these negative effects by reinforcing gut structure and modulating the microbial community. Collectively, these findings underscore the critical role of the gut microbiota in mediating host responses to environmental changes. Future research should therefore focus on elucidating specific toxicological pathways like the gut-organ axis, investigating the transgenerational effects of pollutants, and developing probiotic-based strategies to enhance the resilience and sustainability of aquaculture.

水生环境是一个动态系统，其中多种因素影响宿主与其肠道微生物群之间复杂的相互作用。这篇综述探讨了各种压力源如何改变鱼类和水生无脊椎动物的肠道微生物群，通过检查包括水特性、光周期、外部污染物（如重金属和微塑料）、食物供应和实用的水产养殖饲料添加剂（如乙氧基醌）在内的因素。在这些不同的因素中，出现了共同的模式，包括微生物多样性的破坏，肠道屏障完整性的破坏以及氧化应激的诱导。相反，益生菌和虾青素等有益添加剂通过加强肠道结构和调节微生物群落来减轻这些负面影响。总的来说，这些发现强调了肠道微生物群在介导宿主对环境变化的反应中的关键作用。因此，未来的研究应侧重于阐明特定的毒理学途径，如肠道器官轴，调查污染物的跨代效应，并开发基于益生菌的策略，以增强水产养殖的复原力和可持续性。

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引用次数: 0

Prothioconazole induced stereoselective developmental toxicity and liver injury in zebrafish embryos via ferroptosis 原硫康唑通过铁下垂诱导斑马鱼胚胎立体选择性发育毒性和肝损伤。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-31 DOI: 10.1016/j.cbpc.2025.110380

Jinhao Bian , Hanshuang Zhao , Wenping Xu , Zhong Li , Yang Zhang

The widespread use of prothioconazole (PTCZ), a globally applied triazole fungicide, raises concerns regarding ecological risks from environmental residues and highlights the critical gap in pesticide safety assessment concerning enantiomeric differences in toxicity. This study investigated the stereoselective toxicity and molecular mechanisms of PTCZ enantiomers in an aquatic model using a zebrafish embryo exposure system. The toxic effects were systematically analyzed through multidimensional endpoint assessments, which examined developmental malformations, liver histopathology, lipid metabolism indicators, and lipid peroxidation. The underlying molecular mechanisms were explored through GPX4 immunofluorescence, as well as qPCR and Western blot analyses of ferroptosis-related genes. A ferroptosis inhibitor rescue experiment utilizing Ferrostatin-1 was conducted to investigate the role of ferroptosis in the observed toxicity. Our findings demonstrate that the S-(+)-PTCZ enantiomer induced significantly more severe developmental toxicity and liver injury compared to its counterpart. Mechanistically, S-(+)-PTCZ triggered hepatic damage by activating the lipid peroxidation-ferroptosis axis, as evidenced by inhibition of GPX4 protein expression and an upregulation of the pro-ferroptotic gene acsl4. Crucially, Ferrostatin-1 significantly reversed these effects, reducing lipid peroxidation. Our results confirm that traditional risk assessments based on the racemate (Rac-PTCZ) would substantially underestimate the actual environmental risk posed by the highly non-target bioactive S-(+)-enantiomer. This work provides a critical theoretical basis for the precise regulation and low-toxicity design of chiral pesticides.

prothioconazole （PTCZ）是一种全球通用的三唑类杀菌剂，它的广泛使用引起了人们对环境残留生态风险的关注，并突出了农药安全性评估中对映体毒性差异的关键空白。本研究利用斑马鱼胚胎暴露系统研究了PTCZ对映体在水生模型中的立体选择性毒性及其分子机制。通过多维终点评估系统地分析了毒性作用，包括发育畸形、肝脏组织病理学、脂质代谢指标和脂质过氧化。通过GPX4免疫荧光、qPCR和Western blot分析，探讨了其潜在的分子机制。利用铁抑素-1 （Ferrostatin-1）进行了一项铁下垂抑制剂拯救实验，以研究铁下垂在观察到的毒性中的作用。我们的研究结果表明，S-(+)- ptcz对映体比其对应物诱导更严重的发育毒性和肝损伤。从机制上讲，S-(+)- ptcz通过激活脂质过氧化-铁下垂轴引发肝损伤，这可以通过抑制GPX4蛋白表达和上调前铁下垂基因acsl4来证明。至关重要的是，铁抑素-1显著逆转了这些作用，减少了脂质过氧化。我们的研究结果证实，基于外消旋体（Rac-PTCZ）的传统风险评估将大大低估高度非靶向生物活性S-(+)-对映体所带来的实际环境风险。本研究为手性农药的精确调控和低毒性设计提供了重要的理论依据。

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引用次数: 0

Lipopolysaccharides increase the resorption levels and affect zebrafish scales de novo bone formation 脂多糖增加吸收水平，影响斑马鱼鳞片新生骨形成。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-31 DOI: 10.1016/j.cbpc.2025.110385

Gil Martins , Sunil Poudel , Ana Portela , Gonçalo Pinto , Tamára F. Santos , Francisco A. Guardiola , Ana Marreiros , Paulo J. Gavaia

Zebrafish is a relevant model in skeletal research, enabling insights into bone development and regeneration. Inflammation supports tissue regeneration; however, excessive or chronic inflammation can delay the healing process and contribute to the development of skeletal disorders. Bacterial infections or LPS exposure exacerbate inflammation, hindering bone regeneration. Here, we tested the effects of LPS (1 and 10 μg/mL) as an inducer of an inflammatory response and evaluated its impact on the bone using zebrafish regenerating scales as a model. Results showed that exposure to LPS leads to an inflammatory process that affects scale regenerative ability. Exposure to LPS (10 μg/mL) led to a reduction in scale area, increased scale aspect ratio, osteoclast activity with scale demineralization, as well as overexpression of osteoclastic markers (acp5 and oc-stamp) and downregulation of the osteoblastic marker sp7. Our data suggest that zebrafish regenerating scales exposed to LPS can be further developed as an in vivo screening method to elucidate the mechanisms involved in the increased bone resorption associated with inflammatory processes, to evaluate the effects on osteoblast-osteoclast interaction in fish, and to search for novel therapeutic compounds for skeletal disorders and diseases.

斑马鱼是骨骼研究中的一个相关模型，可以深入了解骨骼的发育和再生。炎症支持组织再生；然而，过度或慢性炎症会延缓愈合过程，并导致骨骼疾病的发展。细菌感染或脂多糖暴露会加剧炎症，阻碍骨再生。在这里，我们测试了LPS（1和10 μg/mL）作为炎症反应诱导剂的作用，并以斑马鱼再生鳞片为模型评估了其对骨骼的影响。结果表明，暴露于LPS会导致炎症过程，影响鳞片再生能力。LPS（10 μg/mL）可导致鳞片面积减少，鳞片长径比增加，破骨细胞活性增加，鳞片脱矿，破骨细胞标志物（acp5和oc-stamp）过表达，成骨细胞标志物sp7下调。我们的数据表明，暴露于LPS下的斑马鱼再生鳞片可以进一步发展为一种体内筛选方法，以阐明与炎症过程相关的骨吸收增加的机制，评估对鱼类成骨细胞-破骨细胞相互作用的影响，并寻找新的治疗骨骼疾病和疾病的化合物。

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引用次数: 0

PFAS-induced immunotoxicity in freshwater fish of inland China: mechanisms and ecological risks pfas诱导的中国内陆淡水鱼免疫毒性：机制和生态风险。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-31 DOI: 10.1016/j.cbpc.2025.110382

Helei Cai , Qizhuan Lin , Changyong Gong , Fan Yu , Libo Jin , Renyi Peng

Per-and polyfluoroalkyl substances (PFAS) are widely distributed across freshwater systems in mainland China, with concentrations showing marked spatial heterogeneity—particularly in eastern regions with intensive industrial activity. Fish not only play a vital ecological role but also serve as an important source of protein for humans. Due to the biomagnification factors (BMF > 1) of PFAS in the food chain, the risk of exposure increases for high-trophic-level fish, other predators, and ultimately humans. Co-exposure with other environmental pollutants further amplifies PFAS-induced immunotoxic effects. The immunotoxicity of PFAS is influenced by carbon chain length and functional groups, with long-chain PFAS and sulfonic acid groups generally exhibiting stronger immunotoxic effects in fish. These compounds significantly suppress both innate and adaptive immune responses by interfering with Toll-like receptor signaling pathways, inducing oxidative stress, and impairing immune cell function. Although current technologies are available for PFAS removal, they still face considerable limitations and challenges. This review summarizes the characteristics and spatial distribution of PFAS contamination in mainland China's aquatic environments and focuses on the mechanisms of PFAS-induced immunotoxicity in fish. It offers valuable insights for future research into the synergistic/antagonistic and time-dependent effects of combined PFAS and multi-pollutant exposure. Moreover, it provides important references for the development of mitigation technologies targeting PFAS-related ecotoxicity in aquatic food chains and for informing relevant policy formulation.

全氟烷基和多氟烷基物质（PFAS）广泛分布在中国大陆的淡水系统中，其浓度表现出明显的空间异质性，特别是在工业活动密集的东部地区。鱼类不仅扮演着重要的生态角色，也是人类重要的蛋白质来源。由于食物链中PFAS的生物放大因子（BMF > 1），高营养水平鱼类、其他捕食者以及最终人类的暴露风险增加。与其他环境污染物的共暴露进一步放大了pfas诱导的免疫毒性作用。PFAS的免疫毒性受碳链长度和官能团的影响，其中长链PFAS和磺酸基团对鱼类的免疫毒性一般较强。这些化合物通过干扰toll样受体信号通路、诱导氧化应激和损害免疫细胞功能，显著抑制先天和适应性免疫反应。尽管目前的技术可用于去除PFAS，但它们仍然面临相当大的局限性和挑战。本文综述了中国大陆水生环境中PFAS污染的特征和空间分布，重点探讨了PFAS诱导鱼类免疫毒性的机制。它为未来研究PFAS和多污染物联合暴露的协同/拮抗效应和时间依赖性提供了有价值的见解。此外，它还为开发针对水生食物链中pfas相关生态毒性的缓解技术和制定相关政策提供了重要参考。

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引用次数: 0

Detoxification metabolic pathways and hepatotoxicity mechanisms of B[a]P in reproductive clam Ruditapes philippinarum B[a]P在繁殖蛤体内的解毒代谢途径及肝毒性机制

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-22 DOI: 10.1016/j.cbpc.2025.110378

Yueyao Zhou, Zhiheng He, Qiuhong Xu, Songhui Xie, Pengfei Li, Qiaoqiao Wang, Jingjing Miao, Luqing Pan

The liver is a major organ of digestion and detoxification metabolism in animals, and the occurrence of most liver diseases is closely associated with environmental pollution. Besides, liver is a non-reproductive primary target organ regulated by sex steroid hormone signaling. In this study, we elucidated the detoxification metabolism pathways of B[a]P in the clam Ruditapes philippinarum and identified the hepatotoxicity mechanism of B[a]P using technical approaches such as transcriptomics, computer simulation and experimental validation. We found that the AhR signaling pathway and CYP450 family (CYP1A1, CYP2B1, CYP3A4) and FMO in the digestive gland of clam play important roles in the detoxification metabolism of B[a]P, but the performance varied between male and female clams. SOD1 and CAT, GPX, PRDX play antioxidant function but PRDX pathway did not function in females. The level of detoxification metabolism in reproductive clams under B[a]P stress was female < male, oxidative stress was female > male, and oxidative damage was female < male. Cell death (apoptosis, pyroptosis and ferroptosis) was aggravated in the digestive gland of both males and females, with a reduced level of hepatic function health and an increase in the level of inflammatory factors, but males presented a more pronounced tendency toward hepatic fibrosis. In summary, the results of this study enrich the research perspectives on the metabolic pathways of POPs in aquatic invertebrates and lay the foundation for the study of POPs-induced hepatotoxicity, which is of great significance for the conservation of marine biological resources and the monitoring of POPs pollution.

肝脏是动物消化和解毒代谢的主要器官，大多数肝脏疾病的发生与环境污染密切相关。此外，肝脏是受性类固醇激素信号调控的非生殖性主要靶器官。本研究通过转录组学、计算机模拟和实验验证等技术手段，阐明了B[a]P在菲律宾蛤（Ruditapes philippinarum）中的解毒代谢途径，并确定了B[a]P的肝毒性机制。我们发现，蛤蜊消化腺中的AhR信号通路、CYP450家族（CYP1A1、CYP2B1、CYP3A4）和FMO在B[a]P的解毒代谢中发挥重要作用，但雌雄蛤蜊的表现不同。SOD1和CAT、GPX、PRDX具有抗氧化作用，而PRDX通路在雌性中不起作用。生殖蛤在B[a]P胁迫下解毒代谢水平为雌性<雄性，氧化应激为雌性>雄性，氧化损伤为雌性<雄性。男性和女性消化腺细胞死亡（凋亡、焦亡和铁亡）加重，肝功能健康水平降低，炎症因子水平升高，但男性肝纤维化倾向更明显。综上所述，本研究结果丰富了POPs在水生无脊椎动物体内代谢途径的研究视角，为POPs致肝毒性研究奠定了基础，对海洋生物资源保护和POPs污染监测具有重要意义。

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引用次数: 0

Combined effects of cadmium and antibiotics on the immune defense system of the wolf spider Pardosa pseudoannulata 镉和抗生素对假环狼蛛免疫防御系统的联合影响。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-20 DOI: 10.1016/j.cbpc.2025.110376

Juan Wang , Yingying Lu , Xinyi Wu , Xinru Zhang , Qu Cai , Chenbo Huang , Zhi Wang

This study investigated the compound stresses of cadmium (Cd) and antibiotics on the immune defense system of wolf spiders, Pardosa pseudoannulata, which is a vital predator for pest control in the paddy ecosystem. Overall, the synergistic effect of Cd and antibiotics on the inhibition of immune response was identified in spiderlings, which manifested by decreased resistance to a pathogen, reduced concentration of immune effectors, and altered concentrations of antioxidants. Similarly, changes in the relative abundances of several antimicrobial peptides, such as lycosins, lycotoxins, and pardosins, were consistent with the change of immune effectors. Specific responsive genes of compound stresses suggested a broader disturbance in the immune system, like the antioxidase system, phenoloxidase, and lysosome, as well as changes in the developmental events like the biosynthesis of molting and juvenile hormones in spiderlings, which were acknowledged as potential contributors to the combined exposure toxicity. Another group of genes involved in ribosome, energy metabolism, and phagosome can act as co-damage markers in response to alone or combined exposures of Cd and antibiotics. These results expanded the insights into the ecotoxicology assessment of combined pollutants in the paddy field.

研究了镉（Cd）和抗生素复合胁迫对稻田生态系统中重要害虫捕食者假环狼蛛（Pardosa pseudoannulata）免疫防御系统的影响。总之，Cd和抗生素在抑制蜘蛛免疫应答方面具有协同作用，表现为对病原体的抵抗力降低，免疫效应物浓度降低，抗氧化剂浓度改变。同样，一些抗菌肽，如lycoins、lycotoxins和pardosin的相对丰度的变化与免疫效应物的变化是一致的。复合应激的特异性应答基因提示免疫系统受到更广泛的干扰，如抗氧化酶系统、酚氧化酶和溶酶体，以及发育事件的变化，如蜘蛛蜕皮和幼崽激素的生物合成，这些都被认为是联合暴露毒性的潜在贡献者。另一组涉及核糖体、能量代谢和吞噬体的基因可以作为单独或联合暴露于Cd和抗生素的共同损伤标记物。这些结果扩大了对稻田复合污染物生态毒理学评价的认识。

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