Comparative Biochemistry and Physiology C-toxicology & Pharmacology最新文献_第9页

Carbamate insecticide bendiocarb induces complex embryotoxic effects, including morphological, behavioral, transcriptional, and immunological alterations in zebrafish 氨基甲酸酯类杀虫剂苯二威在斑马鱼中诱导复杂的胚胎毒性作用，包括形态、行为、转录和免疫改变。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-10 DOI: 10.1016/j.cbpc.2025.110368

Bence Ivánovics , Gyöngyi Gazsi , Zoltán K. Varga , Ádám Staszny , Eszter Váradi , Zsófia Varga , András Ács , Márta Tóth , Apolka Domokos , Márta Reining , Erna Vásárhelyi , Szilárd Póliska , Róbert Kovács , Ferenc Baska , Zoltán Filep , Attila Bácsi , Julianna Kobolák , Béla Urbányi , István Szabó , Tamás Müller , Zsolt Czimmerer

The emergence and spread of vector-borne diseases necessitate the increased use of insecticides, such as carbamates, raising concerns about their potential toxicological risks to non-target organisms, including humans. Bendiocarb, frequently applied in indoor spraying operations and detected in maternal and fetal circulation, warrants particular attention for its developmental toxicity. This study aimed to assess transcriptional and phenotypic effects of sublethal bendiocarb exposure at concentrations of 0.035, 0.2, 0.4, 0.75, and 1.5 mg/L, using zebrafish embryos, a vertebrate model for developmental toxicity testing. Our analyses revealed acetylcholinesterase inhibition-associated morphological and behavioral abnormalities, including reduced locomotor activity in response to both visual and tactile stimuli, as well as impaired non-associative learning. Transcriptomic analysis indicated activation of muscle, immune, and metabolic pathways, while neurodevelopmental, phototransduction, and cell proliferation processes were suppressed. Consistent with these molecular findings, structural damage was observed in the retina, skeletal muscle, and notochord. Furthermore, bendiocarb exposure disrupted neutrophil granulocyte distribution and impaired inflammatory responses. Altogether, our results provide new insights into the embryotoxic effects of bendiocarb, highlighting its potential to disrupt early vertebrate development. These findings provide mechanistic insight that may support more informed evaluations of potential public health risks associated with developmental exposure to carbamates.

由于病媒传播疾病的出现和传播，必须更多地使用氨基甲酸酯等杀虫剂，这引起了人们对它们对包括人类在内的非目标生物的潜在毒性风险的关注。恶虫威经常用于室内喷洒作业，并在母体和胎儿循环中检测到，其发育毒性值得特别注意。本研究旨在评估浓度为0.035、0.2、0.4、0.75和1.5 mg/L的亚致死苯二威暴露对斑马鱼胚胎（一种用于发育毒性测试的脊椎动物模型）的转录和表型影响。我们的分析揭示了乙酰胆碱酯酶抑制相关的形态和行为异常，包括对视觉和触觉刺激的运动活动减少，以及非联想学习受损。转录组学分析表明，肌肉、免疫和代谢途径被激活，而神经发育、光传导和细胞增殖过程被抑制。与这些分子发现一致，在视网膜、骨骼肌和脊索中观察到结构损伤。此外，苯并威暴露会破坏中性粒细胞的分布并损害炎症反应。总之，我们的研究结果为苯虫威的胚胎毒性作用提供了新的见解，突出了其破坏早期脊椎动物发育的潜力。这些发现提供了机制上的见解，可能支持对与发育暴露于氨基甲酸酯相关的潜在公共卫生风险进行更明智的评估。

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引用次数: 0

Effects of cypermethrin on the clams Ruditapes philippinarum (A. Adams & Reeve, 1850) 氯氰菊酯对菲律宾蛤的影响（A. Adams & Reeve, 1850）。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-02 DOI: 10.1016/j.cbpc.2025.110366

Lihua Qi , Chengang Hua , Zongqi Ye , Xu Shen , Yuefeng Cai , Xin Shen

Cypermethrin, highly toxic to aquatic life, impacts Ruditapes philippinarum (A. Adams & Reeve, 1850), an economically valuable species. Thus, studying its toxicity mechanism to R. philippinarum is crucial. In this research, R. philippinarum was exposed to 5000 ng/L cypermethrin and sampled on days 0, 1, 3, 6, 10, 15, and 21 to assess enzymatic activities and conduct transcriptome sequencing. The enzymatic activity results showed that cypermethrin could increase the activity of the ethoxyresorufin-O-deethylase (EROD), turn on the antioxidant defenses, cause an initial increase in total antioxidant capacity (T-AOC) and then a decrease, increase the activities of antioxidant enzymes catalase (CAT) and glutathione peroxidase (GPx), and inhibit superoxide dismutase (SOD). Furthermore, the contents of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) increased in the body at 10, 15, and 21 days of exposure. Transcriptome sequencing was carried out to analyze the responses of cypermethrin stress for 1 day and 21 days. Differentially expressed genes (DEGs) were linked to detoxification metabolism and oxidative stress, according to Gene Ontology (GO) enrichment analysis. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis demonstrated that cypermethrin had toxic effects through key factors affecting detoxification metabolism, oxidative stress, immune response, and apoptosis-related pathways. This study gives insights into the harmful mechanism of cypermethrin stress on R. philippinarum, as well as a theoretical basis for assessing the ecological danger of cypermethrin in Chinese coastal waters.

氯氰菊酯对水生生物有剧毒，对菲律宾Ruditapes philippinarum （A. Adams & Reeve, 1850）具有经济价值。因此，对其毒性机制的研究具有重要意义。本研究采用5000 ng/L的氯氰菊酯对菲律宾褐藻进行处理，并于第0、1、3、6、10、15和21天取样，评估其酶活性并进行转录组测序。酶活性研究结果表明，氯氰菊酯能提高乙氧基间苯二酚- o -去乙基酶（EROD）活性，开启抗氧化防御，使总抗氧化能力（T-AOC）先升高后降低，提高过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性，抑制超氧化物歧化酶（SOD）活性。此外，暴露后10、15和21 天，体内丙二醛（MDA）和8-羟基脱氧鸟苷（8-OHdG）含量增加。转录组测序分析1 d和21 d对氯氰菊酯胁迫的响应。根据基因本体（GO）富集分析，差异表达基因（DEGs）与解毒代谢和氧化应激有关。此外，京都基因与基因组百科（KEGG）富集分析表明，氯氰菊酯通过影响解毒代谢、氧化应激、免疫反应和细胞凋亡相关途径的关键因素具有毒性作用。本研究揭示了氯氰菊酯胁迫对菲律宾绒螯蟹的危害机理，为我国沿海水域氯氰菊酯生态危害评价提供了理论依据。

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引用次数: 0

miR-23a modulates chlorantraniliprole susceptibility by targeting SfGSTs3 in Spodoptera frugiperda (Smith) miR-23a通过靶向frugiperda中的SfGSTs3调节氯虫腈的敏感性（Smith）。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-10-02 DOI: 10.1016/j.cbpc.2025.110367

Fan-Bin Kong , Yong-Po Lv , Bai-Zhong Zhang , Meng-Yuan Zhang , Ling-Ling Cui , Ren-Jie Li , Dong-Mei Chen , Yu-Yang Peng , Run-Qiang Liu

The fall armyworm Spodoptera frugiperda (Smith) can damage many crops distributed worldwide, and chemical insecticide application is the main control strategy. However, the frequent application of insecticides can lead to severe insecticide resistance in S. frugiperda. Glutathione S-transferases (GSTs) play a critical role in insecticide resistance in pests. In this study, it was found that the expression of SfGSTs3 was significantly up-regulated after exposure to chlorantraniliprole. After injection of dsSfGSTs3, the susceptibility of chlorantraniliprole was improved, and microRNA-23a binding on 3’UTR of SfGSTs3 was found. Luciferase reporter assays revealed that the effects of miR-23a on SfGSTs3 expression were suppressed via this binding site in S. frugiperda. Injection of the miR-23a agomir significantly reduced SfGSTs3 expression, together with increased chlorantraniliprole susceptibility. In contrast, injection of the miR-23a antagomir significantly improved SfGSTs3 expression and thus decreased chlorantraniliprole susceptibility in the larvae of S. frugiperda. These findings provide a theoretical foundation for better understanding the posttranscriptional regulation of SfGSTs3 and can be used to further study the mechanism by which miRNAs regulate insecticide susceptibility in pests.

秋粘虫（Spodoptera frugiperda, Smith）是一种危害多种作物的害虫，化学杀虫剂是其主要的防治策略。然而，频繁使用杀虫剂会导致果叶蝉产生严重的抗药性。谷胱甘肽s -转移酶（GSTs）在害虫对杀虫剂的抗性中起着至关重要的作用。本研究发现，暴露于氯虫腈后，SfGSTs3的表达显著上调。注射dsSfGSTs3后，对氯虫腈的敏感性提高，发现microRNA-23a结合在SfGSTs3的3'UTR上。荧光素酶报告基因检测显示，miR-23a对SfGSTs3表达的影响通过这个结合位点在S. frugiperda中被抑制。注射miR-23a agomir可显著降低SfGSTs3的表达，同时增加氯虫腈的敏感性。相比之下，注射miR-23a拮抗剂可显著提高SfGSTs3的表达，从而降低果螨幼虫对氯虫酰胺的敏感性。这些发现为更好地理解SfGSTs3的转录后调控提供了理论基础，并可用于进一步研究miRNAs调控害虫杀虫剂敏感性的机制。

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引用次数: 0

Effects of bisphenol A on freshwater mussels: Insights into the protective mechanisms of selenium 双酚A对淡水贻贝的影响：硒的保护机制

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-09-24 DOI: 10.1016/j.cbpc.2025.110362

Oumaima Abidi , Abdelhafidh Khazri , Rihab Belgacem , Tamara García-Barrera , Ezzeddine Mahmoudi , Mohamed Dellali

Bisphenol A (BPA), a widespread environmental pollutant, is known for its endocrine-disrupting and pro-oxidant effects, posing serious risks to aquatic organisms. This study evaluated the impact of BPA on biochemical markers in the digestive gland of the freshwater mussel Unio ravoisieri, and assessed the potential protective role of selenium (Se), a trace element with antioxidant properties. Under laboratory conditions, mussels were exposed for 14 days to two BPA concentrations (C₁ = 10 μg/L and C₂ = 100 μg/L), either alone or combined with 100 μg/L Se. The enzymatic activity of acetylcholinesterase (AChE), catalase (CAT), and glutathione S-transferase (GST), along with the levels of malondialdehyde (MDA), reduced glutathione (GSH), and hydrogen peroxide (H₂O₂), were quantified as indicators of neurotoxicity and oxidative stress. BPA exposure led to a significant dose-dependent inhibition of AChE activity, indicating neurotoxicity, while GST activity increased, suggesting an upregulated detoxification response. An increase in CAT activity was observed at the lower BPA dose, while inhibition occurred at the higher dose. Importantly, BPA significantly GSH levels, indicating oxidative stress and impaired antioxidant and detoxification defenses. Additionally, BPA exposure at both 10 μg/L and 100 μg/L significantly elevated MDA and H₂O₂ levels, with a more pronounced increase observed at the higher dose (100 μg/L), confirming enhanced lipid peroxidation and reactive oxygen species (ROS). Notably, Se co-treatment mitigated these adverse effects by partially restoring AChE and CAT activities, normalizing GST responses, preserving GSH content, and reducing oxidative damage. These findings demonstrate the intertwined oxidative and detoxification responses of U. ravoisieri to BPA exposure and underscore the protective role of Se in counteracting BPA-induced toxicity. This study reinforces the utility of biochemical biomarkers in environmental monitoring and supports the potential use of Se in pollution mitigation strategies.

双酚A （BPA）是一种广泛存在的环境污染物，具有内分泌干扰和促氧化作用，对水生生物构成严重威胁。本研究评估了BPA对淡水贻贝消化腺生化指标的影响，并评估了具有抗氧化特性的微量元素硒（Se）的潜在保护作用。在实验室条件下，贻贝暴露于两种浓度的BPA （C1 = 10 μg/L和C2 = 100 μg/L）中，分别单独或与100 μg/L Se联合暴露14天。乙酰胆碱酯酶（AChE）、过氧化氢酶（CAT）和谷胱甘肽s -转移酶（GST）的酶活性以及丙二醛（MDA）、还原性谷胱甘肽（GSH）和过氧化氢（H₂O₂）的水平被量化为神经毒性和氧化应激的指标。BPA暴露导致乙酰胆碱酯酶活性明显的剂量依赖性抑制，表明神经毒性，而GST活性增加，表明解毒反应上调。在较低BPA剂量下观察到CAT活性增加，而在较高剂量下则发生抑制。重要的是，BPA显著GSH水平，表明氧化应激和抗氧化和解毒防御受损。此外，BPA暴露在10 μg/L和100 μg/L时，MDA和H₂O₂水平均显著升高，剂量越高（100 μg/L）， MDA和H₂O₂水平升高越明显，证实了脂质过氧化和活性氧（ROS）的增强。值得注意的是，硒共处理通过部分恢复AChE和CAT活性、使GST反应正常化、保留GSH含量和减少氧化损伤，减轻了这些不利影响。这些研究结果表明，暴露在双酚a下的乌氏菌具有复杂的氧化和解毒反应，并强调了硒在对抗双酚a诱导的毒性中的保护作用。该研究加强了生物化学标志物在环境监测中的应用，并支持硒在污染缓解策略中的潜在应用。

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引用次数: 0

Differential toxic phenotypes and liver injury induced by Atractylenolides (I, II, and III): Insights from zebrafish (Danio rerio) models and network toxicology 苍术内酯（I， II和III）诱导的不同毒性表型和肝损伤：来自斑马鱼（Danio rerio）模型和网络毒理学的见解

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-09-23 DOI: 10.1016/j.cbpc.2025.110365

Yue Li , Zihao Jiang , Zhuoshuo Zhou , Naitian Zhang , Ximing Cui , Xiaoyan Yu , Yanli Zhao , Zhong Wang , Jinlian Li , Dongmei Wu

Atractylenolides (I, II, and III), active sesquiterpene lactones from Atractylodes macrocephala Koidz, exhibit diverse pharmacological activities but have been reported to impair drug-metabolizing enzymes and hepatocellular function. However, a comprehensive safety assessment of these compounds remains lacking. In this study, we investigated the developmental toxicity profile of Atractylenolides (I, II, and III) in zebrafish embryos, with a particular focus on hepatotoxicity and its underlying mechanisms. Exposure to Atractylenolides (I, II, and III) resulted in concentration-dependent mortality, with 96-h median lethal concentrations (LC₅₀) of 81.64 μM, 138.40 μM, and 151.90 μM, respectively. Atractylenolides (I, II) induced multiple developmental abnormalities, among which Atractylenolide-I uniquely led to neuronal developmental arrest and diminished locomotor activity. Importantly, Atractylenolides (I, II) exhibited marked hepatotoxicity, evidenced by liver shrinkage, reduced liver-specific fluorescence intensity, and elevated levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). In contrast, exposure to Atractylenolide-III did not induce significant toxic effects. Network toxicology analysis revealed that cytochrome P450 (CYP450) metabolism and apoptosis were closely associated with Atractylenolides (I, II) induced hepatotoxicity. qRT-PCR analysis revealed that Atractylenolides (I, II) suppressed mRNA expression of key drug-metabolizing genes, including cyp3c1 and cyp3a65. Simultaneously, Atractylenolides (I, II) downregulated genes associated with cell proliferation (top2α, uhrf1). Co-treatment with the hepatoprotective agent silybin partially reversed the liver injury and the alterations in drug metabolism gene expression induced by Atractylenolides (I, II). Collectively, our results provide important insights into the safety evaluation of Atractylenolides (I, II, and III).

苍术内酯（I， II和III）是来自苍术的活性倍半萜内酯，具有多种药理活性，但有报道称会损害药物代谢酶和肝细胞功能。然而，对这些化合物的综合安全性评估仍然缺乏。在这项研究中，我们研究了白术内酯（I， II和III）在斑马鱼胚胎中的发育毒性，特别关注肝毒性及其潜在机制。暴露于苍术内酯（I， II和III）导致浓度依赖的死亡率，96小时的中位致死浓度（LC₅0）分别为81.64 μM， 138.40 μM和151.90 μM。苍术内酯（I， II）诱导多种发育异常，其中苍术内酯-I唯一导致神经元发育停滞和运动活动减少。重要的是，苍术内酯（I， II）表现出明显的肝毒性，肝脏萎缩，肝脏特异性荧光强度降低，天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平升高。相比之下，暴露于苍术内酯- iii没有引起明显的毒性作用。网络毒理学分析显示，细胞色素P450 （CYP450）代谢和凋亡与苍术内酯（I， II）引起的肝毒性密切相关。qRT-PCR分析显示，苍术内酯（I， II）抑制了关键药物代谢基因cyp3c1和cyp3a65的mRNA表达。同时，苍术内酯（I， II）下调与细胞增殖相关的基因（top2α, uhrf1）。与保肝剂水飞蓟宾共同治疗可部分逆转苍术内酯引起的肝损伤和药物代谢基因表达的改变（I， II）。总的来说，我们的结果为白术内酯（I， II和III）的安全性评估提供了重要的见解。

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引用次数: 0

Amitriptyline and nortriptyline induce ocular toxicity in early life stage zebrafish (Danio rerio) 阿米替林和去甲替林可引起早期斑马鱼的眼部毒性。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-09-23 DOI: 10.1016/j.cbpc.2025.110363

Marwin Jafari , Jason T. Magnuson , Fabian Essfeld , Sebastian Eilebrecht , Katharina Brotzmann , Daniela M. Pampanin

The global use of antidepressants has steadily increased, raising concern to aquatic ecosystems due to the incomplete removal during wastewater treatment. Tricyclic antidepressants (TCAs) act on the neuronal system by inhibiting the reuptake of serotonin and norepinephrine. However, despite visual function being heavily dependent on the neuronal system, a knowledge gap remains regarding the ocular toxicity of TCAs. To bridge this knowledge gap, zebrafish (Danio rerio) embryos were exposed to sublethal test concentrations of amitriptyline (AMI, 0.3 to 300 μg/L nominal, 2.04 to 234 μg/L measured) and nortriptyline (NOR, 0.03 to 300 μg/L nominal, >0.107 to 20.7 μg/L measured), with the lowest test concentrations being environmentally relevant. Visual function was assessed with the optokinetic response assay, eye structure development was assessed histologically, and gene expression changes were analysed via transcriptomic profiling. Larval zebrafish (120 h post fertilization (hpf)) exposed to 4.99 and 234 μg/L of AMI exhibited a 26 % and 86 % decrease in the number of eye saccades respectively, with zebrafish exposed to 20.7 μg/L of NOR exhibiting a 65 % decrease. Histological analysis indicated a significant increase of the retinal pigment epithelium thickness after exposure to 234 μg/L of AMI and 20.7 μg/L of NOR. Transcriptomic analysis resulted in 1207 and 2742 differentially expressed genes across both AMI and NOR treatment groups respectively, including genes involved in vision, synaptic signaling, and neuronal development. These findings demonstrate that sublethal concentrations of AMI and NOR affect early life stage zebrafish visual development, which may be sensitive endpoint that could be incorporated into ecological risk assessments.

抗抑郁药的全球使用稳步增加，由于废水处理过程中不完全去除，引起了对水生生态系统的关注。三环抗抑郁药（TCAs）通过抑制血清素和去甲肾上腺素的再摄取作用于神经系统。然而，尽管视觉功能严重依赖于神经元系统，但关于TCAs的眼毒性仍然存在知识差距。为了弥补这一知识差距，斑马鱼（Danio rerio）胚胎暴露于亚致死试验浓度的阿米替林（AMI， 0.3至300 μg/L标称，2.04至234 μg/L测量）和去甲替林（NOR， 0.03至300 μg/L标称，bb0 0.107至20.7 μg/L测量），最低试验浓度与环境有关。用光动力学反应法评估视觉功能，用组织学评估眼结构发育，并通过转录组学分析基因表达变化。在受精后120 h （hpf）仔鱼中，AMI浓度分别为4.99和234 μg/L时，其眼跳次数分别减少了26% %和86% %，而NOR浓度为20.7 μg/L时，其眼跳次数减少了65 %。组织学分析显示，AMI浓度为234 μg/L， NOR浓度为20.7 μg/L后，视网膜色素上皮厚度显著增加。转录组学分析结果显示，AMI和NOR治疗组分别有1207个和2742个差异表达基因，包括与视觉、突触信号和神经元发育有关的基因。这些发现表明，亚致死浓度的AMI和NOR会影响斑马鱼生命早期的视觉发育，这可能是一个敏感的终点，可以纳入生态风险评估。

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引用次数: 0

Endotoxin lipopolysaccharide challenge triggers gut microbiota dysbiosis and host immune remodeling in the tiger grouper Epinephelus fuscoguttatus 内毒素脂多糖挑战引发斑石斑鱼肠道菌群失调和宿主免疫重塑。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-09-23 DOI: 10.1016/j.cbpc.2025.110364

Yafei Duan , Yukai Yang , Ruijie Zhu , Meng Xiao

Lipopolysaccharide (LPS) is a bacterial endotoxin that can trigger immune responses in aquatic animals. The gut microbiota is crucial for nutrient absorption and immune regulation in fish. In this study, the tiger grouper Epinephelus fuscoguttatus was selected as an animal model to investigate the effects of LPS challenge on the composition and function of gut microbiota and the gene transcriptional profiles of head kidney. The results showed that LPS challenge slightly increased gut microbial diversity indices (ACE, Simpson) and decreased Chao1 and Shannon indices; it also increased the abundances of Proteobacteria and harmful bacteria (Vibrio, Pseudomonas), while decreased those of Firmicutes, Actinobacteria and beneficial bacteria (Collinsella, Romboutsia) that produce short-chain fatty acids. The predicted carbohydrate and protein digestion/absorption functions of gut microbiota were significantly weakened. Additionally, LPS challenge induced 757 up- and 379 down-regulated genes in the head kidney, mainly involved in DNA replication, aminoacyl-tRNA biosynthesis and two-component system. Immune-related genes including pattern recognition receptors TLR1/2 was up-regulated but TLR3 was down-regulated; immune regulation (PIgR, MHC2), apoptosis (Cytc, Casp3, BIRC5), stress response (HSP60/70/90, GRP94, Bip, HURP1) and iron homeostasis (Mfn1, TFR1, Hepc) related genes were up-regulated; complement (C1QL3, C1QBP, C7), chemokines and their receptors (LECT2, CXCR3/1/4, CCR2) related genes showed disordered expression. Several bacterial genera groups, such as Collinsella, Romboutsia, Phyllobacterium, Prevotella 9, and Prevotellaceae NK3B31, were positively associated with the changes in host immune function. These findings demonstrated that LPS challenge induced the structural perturbations of gut microbiota, and disrupted the immune homeostasis of the head kidney of the grouper.

脂多糖（LPS）是一种细菌内毒素，可以引发水生动物的免疫反应。肠道菌群对鱼类的营养吸收和免疫调节至关重要。本研究以斑石斑鱼（Epinephelus fuscoguttatus）为动物模型，研究LPS对斑石斑鱼肠道菌群组成、功能及头肾基因转录谱的影响。结果表明：LPS刺激使肠道微生物多样性指数（ACE、Chao1、Simpson）略有升高，Shannon指数略有降低；它还增加了变形菌门和有害细菌（弧菌，假单胞菌）的丰度，而减少了厚壁菌门，放线菌门和产生短链脂肪酸的有益细菌（Collinsella, Romboutsia）的丰度。预测肠道菌群的碳水化合物和蛋白质消化吸收功能显著减弱。此外，LPS诱导头肾757个上调基因和379个下调基因，主要涉及DNA复制、氨基酰基- trna生物合成和双组分系统。模式识别受体TLR1/2等免疫相关基因上调，TLR3下调；免疫调节（PIgR、MHC2）、凋亡（Cytc、Casp3、BIRC5）、应激反应（HSP60/70/90、GRP94、Bip、HURP1）和铁稳态（Mfn1、TFR1、Hepc）相关基因上调；补体（C1QL3、C1QBP、C7）、趋化因子及其受体（LECT2、CXCR3/1/4、CCR2）相关基因表达紊乱。Collinsella、Romboutsia、Phyllobacterium、Prevotella 9和Prevotellaceae NK3B31等细菌属群与宿主免疫功能的变化呈正相关。这些结果表明，LPS刺激引起了石斑鱼肠道微生物群的结构扰动，并破坏了石斑鱼头肾的免疫稳态。

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引用次数: 0

Parental exposure to CdSe/ZnS QDs affects otolith development and locomotor behavior in rare minnow (Gobiocypris rarus) offspring 父母暴露于CdSe/ZnS QDs会影响稀有鲦鱼后代的耳石发育和运动行为。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-09-20 DOI: 10.1016/j.cbpc.2025.110360

Weiwei Xie , Qiaoqiao Zhang , Li Jin

Quantum dots (QDs) influence otolith development and locomotor behavior, although the underlying mechanism remain unclear. This study evaluated the toxicity of CdSe/ZnS QDs on otolith development and locomotor behavior in the next generation (F1) using rare minnows (Gobiocypris rarus) as model organisms. The sexually mature parents of rare minnows (F0) were exposed to varying concentrations of CdSe/ZnS QDs (0, 100, 200, 400 and 800 nmol/L) for four days, after which embryos were collected through artificial insemination. The results indicated that exposure to varying concentrations of QDs inhibited the growth of utricular and saccular otoliths in F1 larvae, evidenced by a decrease in otolith lateral area. Furthermore, an increase in malformations and mortality rate were observed, alongside reductions in swimming speed, movement distance and overall calcium content, ultimately resulting in loss of balance control in F1 larvae. Exposure to CdSe/ZnS QDs also suppressed the transcript expression of the atp2b1a, stm and sparc genes, while promoting the transcription of cahz, ecac and omp1 genes. Notably, the adverse effects of CdSe/ZnS QDs on otolith growth and swimming behavior could be mitigated by supplementing Ca²⁺ in the environment. Following calcium supplementation, the damage to otolith development in larvae was alleviated to some extent, and locomotor behavior returned to normal. Overall, the results suggest that CdSe/ZnS QDs can adversely affect otolith development and locomotor behavior in F1 larvae of rare minnows at both individual and molecular levels, ultimately leading to impaired swimming abilities and balance control.

量子点（QDs）影响耳石发育和运动行为，尽管潜在的机制尚不清楚。本研究以稀有鲦鱼（Gobiocypris rarus）为模型生物，评估了CdSe/ZnS量子点对下一代（F1）耳石发育和运动行为的毒性。将不同浓度的CdSe/ZnS QDs（0、100、200、400和800 nmol/L）处理4 d，人工授精收集胚胎。结果表明，不同浓度的QDs对F1幼虫的卵状和囊状耳石生长均有抑制作用，耳石外侧面积减小。此外，观察到畸形和死亡率的增加，以及游泳速度、移动距离和总体钙含量的降低，最终导致F1幼虫失去平衡控制。暴露于CdSe/ZnS QDs还抑制了atp2b1a、stm和sparc基因的转录表达，同时促进了cahz、ecac和omp1基因的转录。值得注意的是，CdSe/ZnS量子点对耳石生长和游泳行为的不利影响可以通过在环境中补充Ca2+来缓解。补钙后，幼体耳石发育损伤有所减轻，运动行为恢复正常。综上所述，CdSe/ZnS量子点可在个体和分子水平上对稀有鲦鱼F1幼虫的耳石发育和运动行为产生不利影响，最终导致游泳能力和平衡控制能力受损。

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引用次数: 0

Developmental toxicity of functionalized polystyrene microplastics and their inhibitory effects on fin regeneration in zebrafish 功能化聚苯乙烯微塑料的发育毒性及其对斑马鱼鳍再生的抑制作用。

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-09-19 DOI: 10.1016/j.cbpc.2025.110358

Qian Li , Wenjing Cheng , Hongyu Wang , Jun Chen , Yueyun Qin , Ruozhu Sheng , Shaohong Peng , Zaitian Li , Tao Lu , Liwei Sun

The hazardous effects of microplastics on aquatic biota have been extensively documented. However, the impact of surface functionalization on the toxicological profiles of microplastics, particularly in fish, requires further investigation. In the present study, the effects of polystyrene microplastics (50–100 nm in diameter), either non-functionalized or functionalized with carboxyl (-COOH) or amino (−NH₂) groups, on zebrafish were explored. Exposure to all three types of microplastics (0.1, 1, and 10 mg/L) resulted in developmental toxicity in zebrafish at early life stages. This included increased mortality, reduced larval body length, decreased heart rate, impaired swimming ability, as well as disruptions in redox homeostasis, nervous system development, and immune responses. Moreover, all types of microplastics (1 mg/L) inhibited the regenerative capacity of larval zebrafish after caudal fin amputation, both morphologically and functionally. This inhibition was likely due to alterations in immune response, excessive production of reactive oxygen species, and disruptions in the signaling networks that regulate fin regeneration. Among the tested microplastics, amino-modified particles generally exhibited greater developmental toxicity and stronger inhibitory effects on fin regeneration. Our findings confirm that the functionalization of microplastics plays a crucial role in their interactions with biological systems and highlight concerns regarding the potential effects of functionalization, whether intentional or resulting from natural weathering processes.

微塑料对水生生物群的有害影响已被广泛记载。然而，表面功能化对微塑料毒理学特征的影响，特别是对鱼类的影响，需要进一步研究。在本研究中，研究了聚苯乙烯微塑料（直径50-100 nm），无论是非官能团还是羧基（-COOH）或氨基（-NH2）官能团，对斑马鱼的影响。暴露于所有三种类型的微塑料（0.1、1和10 mg/L）会导致斑马鱼在生命早期出现发育毒性。这包括死亡率增加，幼虫体长缩短，心率下降，游泳能力受损，以及氧化还原稳态，神经系统发育和免疫反应的破坏。此外，所有类型的微塑料（1 mg/L）在形态和功能上都抑制了斑马鱼尾鳍切除后的再生能力。这种抑制可能是由于免疫反应的改变、活性氧的过量产生以及调节鳍再生的信号网络的中断。在所测试的微塑料中，氨基修饰颗粒普遍表现出更大的发育毒性和更强的鳍再生抑制作用。我们的研究结果证实，微塑料的功能化在它们与生物系统的相互作用中起着至关重要的作用，并强调了功能化的潜在影响，无论是故意的还是自然风化过程的结果。

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引用次数: 0

The plastic threat to marine gastropods: Oxidative stress, energy metabolism and impaired recovery in juvenile abalone (Haliotis discus hannai) exposed to polystyrene microplastics 塑料对海洋腹足类动物的威胁：暴露于聚苯乙烯微塑料的幼鲍鱼（Haliotis discus hannai）的氧化应激、能量代谢和恢复受损

IF 4.3 3区环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology C-toxicology & Pharmacology

Pub Date : 2025-09-19 DOI: 10.1016/j.cbpc.2025.110361

Chaoyi Xie , Hongce Song , Junjie Li , Shuai Xu , Liqun Ren , Chunnuan Zhao , Yuping Wu , Xiaotong Wang , Biao Wu , Shubao Zhang , Haiying Han , Tao Yu

Microplastic (MP) pollution challenges marine ecosystems, with gastropods like Haliotis discus hannai being potentially vulnerable. However, the reversibility of MP-induced physiological damage in gastropods remains poorly understood. This study investigated the effects of polystyrene microplastics (PS-MPs; 0, 100, and 1000 μg/L) on H. discus hannai in a 14-day exposure followed by a 7-day recovery experimental design. The analysis focused on antioxidant status, energy metabolism, and immune pathways. Results showed dose-dependent oxidative stress during PS-MPs exposure, indicated by significantly (P < 0.05) elevated antioxidant enzyme activities (Superoxide dismutase, Catalase and Glutathione peroxidase), which decreased but did not normalize during recovery. Exposure to a low concentration of PS-MPs (100 μg/L) resulted in metabolic adjustments consistent with homeostatic maintenance, whereas a high concentration (1000 μg/L) significantly increased lactate dehydrogenase and Na⁺/K⁺-ATPase activities, suggesting metabolic disruption. These energy metabolism alterations persisted, showing incomplete recovery. Immune pathway analysis revealed minimal changes at low PS-MPs concentrations but significant enrichment of pathways like IL-17 and Fc-Epsilon-RI signaling at high concentrations during exposure. Notably, sustained activation of immune pathways, including NOD-like receptor, Toll-like receptor, and IL-17 signaling (particularly in the high-concentration group), persisted during recovery, potentially indicating prolonged cellular stress or links to apoptosis. Furthermore, Integrated Biomarker Response (IBR) analysis confirmed that H. discus hannai remained severely impacted even after the recovery period. This study provides crucial evidence on the complex toxicodynamics of PS-MPs in H. discus hannai, highlighting incomplete physiological recovery and significant damage during the recovery period following exposure, especially at high concentrations.

微塑料污染对海洋生态系统构成了挑战，像盘藻这样的腹足类动物可能很脆弱。然而，mp诱导的腹足类生理损伤的可逆性仍然知之甚少。本研究研究了聚苯乙烯微塑料（PS-MPs； 0、100和1000 μg/L）在14天的暴露下对虹桃的影响，然后进行了7天的恢复实验设计。分析的重点是抗氧化状态、能量代谢和免疫途径。结果显示，PS-MPs暴露期间存在剂量依赖性氧化应激，表现为抗氧化酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）活性显著（P < 0.05）升高，恢复期间活性下降但未恢复正常。暴露于低浓度PS-MPs （100 μg/L）下导致代谢调节符合稳态维持，而高浓度PS-MPs （1000 μg/L）显著增加乳酸脱氢酶和Na+/K+- atp酶活性，提示代谢紊乱。这些能量代谢改变持续存在，表现为不完全恢复。免疫途径分析显示，低PS-MPs浓度下的变化很小，但高浓度暴露时IL-17和Fc-Epsilon-RI信号通路显著富集。值得注意的是，免疫通路的持续激活，包括nod样受体、toll样受体和IL-17信号传导（特别是在高浓度组），在恢复过程中持续存在，可能表明细胞应激延长或与凋亡有关。此外，综合生物标志物反应（IBR）分析证实，即使在恢复期后，铁饼猴仍然受到严重影响。本研究提供了重要的证据，证明了pps - mps在铁饼鱼体内的复杂毒理学，强调了暴露后的不完全生理恢复和恢复期的显著损害，特别是在高浓度下。

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引用次数: 0