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Plant breeding in Brazil: Retrospective of the past 50 years 巴西植物育种:过去50年的回顾
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-06-21 DOI: 10.1590/1984-70332021v21sa16
M. Ramalho, T. L. Marques, Roxane do Carmo Lemos
Abstract: The importance of plant breeding in Brazilian agriculture has grown a lot in the last 50 years. This occurred mainly because of the: increase in graduate programs, which qualified hundreds of professionals; creation of EMBRAPA and other research institutes or state companies, with an emphasis on the production of new cultivars and; promulgation of the cultivar protection law, which stimulates investments in seed production. The retrospective of what happened, enabling the country to move from being an importer of grains, fruits, and fibers to one of the largest exporters of these products worldwide, was the focus of this work. Taking as reference some agricultural products, this article highlights the significant contribution of plant breeding in recent years. Also, some of the enormous challenges that still have to be overcome, in which the participation of Brazilian breeders will be fundamental to continue the progress of agriculture in the coming years.
摘要:近50年来,植物育种在巴西农业中的重要性不断提高。这主要是因为:研究生项目的增加,使数百名专业人员获得了资格;创建EMBRAPA和其他研究机构或国有公司,重点生产新品种;颁布了品种保护法,刺激了对种子生产的投资。这项工作的重点是回顾所发生的事情,使该国从谷物、水果和纤维的进口国转变为全球最大的这些产品出口国之一。本文以一些农产品为参考,着重介绍了近年来植物育种的重大贡献。此外,一些仍然需要克服的巨大挑战,巴西饲养员的参与将是未来几年农业继续进步的根本。
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引用次数: 7
Environmental stratification and genotype recommendation toward the soybean ideotype: a Bayesian approach 大豆理想型的环境分层和基因型推荐:贝叶斯方法
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-05-21 DOI: 10.1590/1984-70332021V21N1A11
J. S. P. C. Evangelista, M. A. Peixoto, I. Coelho, R. S. Alves, F. F. Silva, M. Resende, F. L. Silva, L. L. Bhering
Abstract The genotype × environment (G×E) interaction plays an essential role in phenotypic expression and can lead to difficulties in genotypes recommendation. Thus, the objectives of this study were: i) propose the Multi-Environment Index Based on Factor Analysis and Ideotype-Design/Markov Chain Monte Carlo (FAI/MCMC index), and ii) apply it for soybean genotypes recommendation. To this end, a data set with 30 soybean genotypes evaluated in 10 environments for grain yield trait was used. Variance components, genetic parameters and genetic values were estimated through MCMC algorithm. Environmental stratification was conducted by factor analyses and the selection of soybean genotypes was performed using the FAI/MCMC index. The results indicated the existence of genotypic variability and G×E interaction. The environments were grouped into three factors. The predicted genetic gains from indirect selection was 4.81%. Thus, our results suggest that the FAI/MCMC index can be successfully used in soybean breeding.
基因型×环境(G×E)相互作用在表型表达中起着重要作用,可能导致基因型推荐困难。因此,本研究的目的是:i)提出基于因子分析和Ideotype Design/Markov Chain Monte Carlo的多环境指数(FAI/MMC指数),以及ii)将其应用于大豆基因型推荐。为此,使用了一个包含30个大豆基因型的数据集,在10个环境中评估了粮食产量性状。通过MCMC算法估计方差分量、遗传参数和遗传值。通过因子分析进行环境分层,并使用FAI/MMCC指数进行大豆基因型的选择。结果表明存在基因型变异和G×E相互作用。环境分为三个因素。间接选择的遗传增益预测值为4.81%,表明FAI/MMCC指数可以成功应用于大豆育种。
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引用次数: 4
SNP genotyping for fast and consistent clustering of maize inbred lines into heterotic groups 玉米自交系快速一致聚类的SNP基因分型
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-05-21 DOI: 10.1590/1984-70332021V21N1A10
Lander Santos de Oliveira, I. Schuster, E. Novaes, W. A. Pereira
Abstract Advances in genotyping technologies have transformed the way breeding programs manage their genetic resources. The identification of single nucleotide polymorphisms (SNPs) can improve understanding of the genetic diversity of maize (Zea mays) inbred lines and their classification into heterotic groups, which is useful in determining certain crosses to obtain hybrids with higher yield performance. The genetic diversity of 293 inbred lines was investigated with 5252 SNPs with minor allele frequency (MAF)>5%. There was an average of 525 SNPs per chromosome. Polymorphism information content (PIC) averaged 0.297. The unweighted pair group method with arithmetic mean analysis (UPGMA) and principal component analysis (PCA) based on the genetic distance matrix revealed four similar clusters and high cophenetic correlation coefficients (0.953 and 0.863, respectively). The results showed consistency between genetic distance-based grouping and the heterotic groups previously established using pedigree and topcross information for the inbred lines studied.
摘要基因分型技术的进步改变了育种项目管理其遗传资源的方式。单核苷酸多态性(SNPs)的鉴定可以提高对玉米自交系遗传多样性及其杂种群分类的理解,这有助于确定某些杂交组合,以获得更高产的杂交种。用5252个等位基因频率(MAF)>5%的SNPs对293个自交系的遗传多样性进行了研究。每个染色体平均有525个SNPs。多态性信息含量(PIC)平均为0.297。基于遗传距离矩阵的算术平均分析(UPGMA)和主成分分析(PCA)的未加权对群方法显示了四个相似的聚类,并且具有很高的协方差相关系数(分别为0.953和0.863)。结果表明,对于所研究的自交系,基于遗传距离的分组与先前使用系谱和顶交信息建立的杂种群之间是一致的。
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引用次数: 0
Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva 合成cry1Ab基因的组成表达赋予了马铃薯块茎蛾(Phthorimaea operculella Zeller)幼虫的抗性
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-05-21 DOI: 10.1590/1984-70332021V21N1A9
H. Salehian, H. Rahnama, S. Dezhsetan, S. Babaei
Abstract The potato tuber moth (Phthorimaea operculella Zeller) (PTM) is a destructive potato pest. Bacillus thuringiensis cry genes encode crystal proteins that have toxic characteristics against PTM. The aim of the present study was to develop a PTM resistant potato cv. Agria using the synthetic Bt cry1Ab gene. The transformation vector was constructed by inserting the cry1Ab gene into the pBI121 binary expression vector. The Agrobacterium tumefaciens strain AGL1 harboring the pBI35SCry1Ab construct was used for potato transformation. The results indicated that out of 97 putative transformed lines, 30 lines were PCR positive. Southern blot analysis revealed that one to three copies of transgenes were inserted into transgenic lines. The immunoassay test revealed the expression of the Cry protein. Furthermore, the results of bioassays confirmed the high mortality level of neonate larvae. The results of this study suggested that the transgenic lines have significant potential in pest control.
摘要马铃薯块茎蛾是一种破坏性的马铃薯害虫。苏云金芽孢杆菌cry基因编码对PTM具有毒性特征的晶体蛋白。本研究的目的是利用合成的Bt cry1Ab基因培育抗PTM的马铃薯品种Agria。将cry1Ab基因插入pBI121二元表达载体中构建转化载体。将携带pBI35SCry1Ab构建体的根癌农杆菌菌株AGL1用于马铃薯转化。结果表明,在97个假定的转化系中,有30个系为PCR阳性。Southern印迹分析显示,将一到三个拷贝的转基因插入转基因系中。免疫测定显示Cry蛋白的表达。此外,生物测定结果证实了新生幼虫的高死亡率。本研究结果表明,转基因株系在害虫防治方面具有显著的潜力。
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引用次数: 1
Evaluation of families derived from backcrosses of processed tomato with dwarfism gene 加工番茄矮化基因回交家系评价
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-05-21 DOI: 10.1590/1984-70332021V21N1N13
P. Y. Cavasin, Leandro Augusto Andrade Fumes, A. R. Fonseca, D. Silva
Abstract In comparison to tomato plants without the dwarfism gene, dwarf tomato plants were shorter, had a smaller canopy diameter and remained upright during maturation, thereby reducing the fruit-soil contact, resulting in healthier fruits. This study evaluated tomato families of a BC1F3 population carrying the dwarf gene for industrial yield. The 150 BC1F3 families and the two parents (hybrid H-9889 and accession BGH-9889) as controls were assessed in an augmented block design with four replications. The data were subjected to variance analysis and the F test. A selection index, based on the sum of ranks of Mulamba and Mock (1978), was used for the selection of genotypes, considering the most relevant traits, i.e., canopy diameter, yield, lodging and fruit firmness. Thus, selection resulted in a yield gain of 8.04%.
摘要与没有矮化基因的番茄植株相比,矮化番茄植株更短,冠层直径更小,在成熟过程中保持直立,从而减少了果土接触,从而获得更健康的果实。本研究评估了携带矮化基因的BC1F3群体的番茄家族的工业产量。150个BC1F3家族和两个亲本(杂交H-9889和登录BGH-9889)作为对照,在具有四个复制的扩增块设计中进行评估。对数据进行方差分析和F检验。选择指数基于Mulamba和Mock(1978)的等级总和,用于基因型的选择,考虑了最相关的性状,即冠层直径、产量、倒伏和果实硬度。因此,选择的产量增加了8.04%。
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引用次数: 2
Phylogenetic inference applied to germplasm bank characterization and interspecific breeding in passionfruit 系统发育推断在百香果种质库鉴定和种间育种中的应用
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-05-21 DOI: 10.1590/1984-70332021V21N1A12
P. Inglis, G. Bellon, N. Junqueira, F. Faleiro, M. E. Ferreira
Abstract Interspecific crosses between the more than 520 Passiflora species may or may not be compatible. The sequences ITS, matK, psbA-trnH, rbcL and trnL-F were used to confirm species identity and to estimate the genetic distances among 48 Passiflora accessions of a germplasm bank. Twenty species were used for crosses within and between distinct Passiflora subgenera. The phylogenetic resolution based on ITS data was superior to that of any single chloroplast marker, recommending it as a DNA barcode for this genus. However, the tree topology based on Bayesian phylogenetic analysis using the combination of the four chloroplast markers possessed greater support for subclades within the subgenus Passiflora, which contains more species of interest for breeders. We could not identify a clear cutoff value of pairwise Kimura two-parameter (K2P) distances to predict success or failure of crosses. Rather, the clustering of species pairs together within the same or closely related phylogenetic subclades predicted the probability of wide cross compatibility more reliably.
摘要520多种西番莲之间的种间杂交可能是相容的,也可能不是相容的。利用ITS、matK、psbA-trnH、rbcL和trnL-F序列对一个种质库的48份西番莲材料进行了物种鉴定和遗传距离估计。20个物种被用于不同西番莲亚属内部和之间的杂交。基于ITS数据的系统发育分辨率优于任何单一叶绿体标记,推荐将其作为该属的DNA条形码。然而,基于贝叶斯系统发育分析的树状拓扑结构使用了四个叶绿体标记的组合,对西番莲亚属中的子属有更大的支持,西番莲亚属包含了更多育种家感兴趣的物种。我们无法确定成对Kimura双参数(K2P)距离的明确截止值来预测杂交的成功或失败。相反,在相同或密切相关的系统发育子系统中,物种配对的聚类更可靠地预测了广泛交叉兼容性的可能性。
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引用次数: 0
Heritability and combined parental information to define the number of crosses in circulant diallels 遗传力和结合亲本信息来确定循环双列的杂交数
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-01 DOI: 10.1590/1984-70332021v21n2c34
Gabriela Inocente, D. D. Garbuglio, P. Araújo, P. Ruas
In many agricultural crops, the number of parents used in cross breeding make it difficult to obtain all possible hybrids in diallels. Therefore, using maize as a model plant and based on traits with high and low heritabilities, the aim of the present study was to quantify the correlation estimates of the general combining ability (GCA) and specific combining ability (SCA) between a complete diallel and a circulant diallel, with and without the inclusion of parents. For the high heritability trait, the GCA estimates can be obtained with low s values, whereas for the SCA estimates, s values close to half the number of parents should be used. For the low heritability trait, information from parents must be used to obtain the SCA estimates. For the GCA estimates, considering the stabilization of r above 0.70 for s=7, s values greater than half the number of parents must be used in the circulant models.
在许多农作物中,杂交育种中使用的亲本数量使获得所有可能的双列杂交品种变得困难。因此,本研究以玉米为模式植物,在高遗传力和低遗传力性状的基础上,对完全双列和循环双列,包括亲本和不包括亲本之间的一般配合力(GCA)和特定配合力(SCA)的相关估计进行了量化。对于高遗传力性状,可以用较低的s值获得GCA估计值,而对于SCA估计值,应该使用接近亲本数量一半的s值。对于低遗传力性状,必须使用来自亲本的信息来获得SCA估计值。对于GCA估计,考虑到s=7时r大于0.70的稳定性,循环模型中必须使用大于父母数量一半的s值。
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引用次数: 1
UENF WS01: popcorn hybrid with water use efficiency for the State of Rio de Janeiro unenf WS01:巴西里约热内卢州用水效率高的爆米花杂交品种
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-01 DOI: 10.1590/1984-70332021v21n4c60
Valter Jário de Lima, A. T. A. Amaral Júnior, S. Kamphorst, Adriano dos Santos, K. Schmidt, V. C. Azeredo, J. T. Leite, Divino Rosa Dos Santos Junior, T. D. O. Santos, R. Bispo, Carolina Macedo Carvalho, Yure Pequeno de Souza, F. T. D. Oliveira, Marcelo Moura Chaves, Uéliton Alves de Oliveira, G. S. Mafra, P. H. Santos
The UENF WS01 is a hybrid that has an early cycle and high grain yield capacity under low water and nitrogen availability. It maintains popping expansion even under abiotic stress, thus being recommended for cultivation in the North and Northwest of the State of Rio de Janeiro.
unenf WS01是一种循环早、低水氮有效度条件下高产的杂交种。它即使在非生物胁迫下也能保持膨大,因此被推荐在巴西里约热内卢州北部和西北部种植。
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引用次数: 4
Obtaining okra hybrids through partial diallel analysis 通过部分双列分析获得秋葵杂交种
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-01 DOI: 10.1590/1984-70332021v21n4a50
E. H. C. Silva, W. Candido, L. T. Braz
The exploration of heterosis can be an efficient alternative to increase okra yield. The partial diallel strategy optimizes resources by guiding crosses based on genetic diversity between various genotypes. This study aimed to identify potential hybrid combinations through diallel analysis in comparison with commercial cultivars. The hybrids were evaluated by means of a partial diallel scheme based on the genetic divergence of the genotypes. Fourteen hybrids and their reciprocals were evaluated in the winter and summer conditions of Jaboticabal – SP for yield, number of fruits and precocity. No line showed significant general combining ability. The reciprocal effect was not significant for the evaluated characteristics. Four hybrid combinations showed significant and positive specific combining ability for number of fruits, which were considered promising hybrids. In the winter, the experimental hybrids 1 x 8 and 3 x 8 showed superior performance compared to the commercial hybrids. No significant differences were observed in the summer.
探索杂种优势是提高秋葵产量的有效途径。部分双列策略是根据不同基因型之间的遗传多样性,通过引导杂交来优化资源。本研究旨在通过与商品品种的双列杂交分析,确定潜在的杂交组合。根据基因型的遗传差异,采用部分双列杂交方法进行鉴定。在冬季和夏季条件下,对14个Jaboticabal - SP杂交种及其后代的产量、果数和早熟性进行了评价。没有品系表现出显著的一般配合力。评价性状的互反效应不显著。4个杂种组合的果实数特异配合力显著且为正,是有发展前途的杂种组合。在冬季,试验杂交种1 × 8和3 × 8表现出优于商品杂交种的性能。在夏季没有观察到显著差异。
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引用次数: 1
Genetic variability and recurrent selection in corn population with potential for green corn production 具有绿玉米生产潜力的玉米群体的遗传变异和循环选择
IF 1.5 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2021-01-01 DOI: 10.1590/1984-70332021v21n4a58
Maraiza Lima Costa, W. Candido, J. Pinto, C. M. Silva, B. Silva, E. F. Reis
The present study selected progenies, aiming at the production of green corn, by methods of intrapopulation recurrent selection with subsequent recombination, generating open-pollinated populations. To this end, in the 2017/2018 second season, progenies from the PMVJ01 population (originated from the recombination of progenies of the TG-02R2 x AG1051 cross) were evaluated, using PMVJ01 and the hybrid AG1051 as controls. The following parameters were evaluated: flowering, heights, green ear yield, husk cover, ear diameter and length, mass yield, ear shape, number of rows on the ear, alignment of rows, color of kernels, and husk width and length. Genetic parameters were estimated, and the gain was predicted with the selection of 20% of the progenies, using the index of Mulamba and Mock in four selection procedures and a fifth by multivariate method. Genetic variability was found in the population, which allows advances in the selection process. After recombination, two of the populations generated showed potential for use as open-pollinated cultivar.
本研究以绿玉米生产为目标,采用群体内循环选择和再组合的方法选育后代,产生开放授粉群体。为此,在2017/2018第二季,以PMVJ01和杂种AG1051为对照,对PMVJ01群体(TG-02R2与AG1051杂交后代重组而来)的后代进行了评价。评估了以下参数:开花、高度、绿穗产量、谷壳覆盖、穗径和穗长、产量、穗形、穗上行数、行排列、籽粒颜色、谷壳宽度和长度。利用Mulamba和Mock在4个选择过程中的指数和5个选择过程中的多变量方法,估计遗传参数,预测20%的后代的增益。在种群中发现了遗传变异,这使得选择过程得以推进。重组后产生的两个群体显示出作为开放授粉品种使用的潜力。
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引用次数: 1
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Crop Breeding and Applied Biotechnology
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