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Electrostatic technology for pesticide application. Prototype characterization and validation in super-intensive olive crops 静电农药应用技术。超集约化橄榄作物的原型特性和验证
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-10-02 DOI: 10.1016/j.cropro.2025.107429
Alba Vigo-Morancho , María Videgain , Sergio Artero , Mariano Vidal , Francisco César Páez-Cano , Julián Sánchez-Hermosilla , Francisco Javier García-Ramos
Electrostatic technology for agricultural crops has been studied in recent decades to improve pesticide deposition by applying voltage to droplets, which maximizes the amount of product reaching the deepest areas of the canopy through attractive forces. However, few studies perform a comprehensive laboratory characterization of a sprayer followed by field validation in order to explore the possibility of reduced amount of chemicals, specifically for super-intensive olive cultivation.
This study aimed to conduct a laboratory evaluation of an electrostatic sprayer prototype designed for three-dimensional hedge crops and validate its performance through field experiments. Sprayer configurations associated with activated or deactivated electrostatic system (ES vs. NES, respectively) were tested. The characterization included airflow evaluation, product distribution profile, charge-to-mass ratio (CMR) quantification, and deposition trials, varying application parameters such as liquid pressure, airflow setup, and distance under controlled environmental conditions. A differentiation between adaxial and abaxial areas of the artificial target during deposition assays was also carried out. Finally, field trials compared the electrostatic sprayer to conventional application methods, providing practical insights into pesticide reduction efficiency. Laboratory results showed no differences in flow rate (L min−1) between ES and NES configurations at any tested pressure. The total accumulated volume from the vertical distribution profile showed variations associated with airflow configuration and liquid pressure as a function of distance. Deposition results revealed higher coverage and deposition when the electrostatic system was activated, regardless of distance or height, with a 70 % increase in abaxial areas compared to the NES. Field trials in super-intensive olive orchards revealed good performance in terms of coverage and deposition on artificial receptors and leaves after application with the electrostatic equipment, particularly at the highest application dose (163.3 L ha−1) compared to the conventional spraying (466.3 L ha−1), highlighting a 60 % reduction in application volume. Additionally, ground losses were significantly lower with the electrostatic sprayer, as coverage and deposition at the base of the trunk were substantially reduced compared to conventional equipment.
近几十年来,人们对农作物静电技术进行了研究,通过对液滴施加电压来改善农药沉积,从而使产品通过吸引力最大限度地到达冠层最深处。然而,很少有研究对喷雾器进行全面的实验室表征,然后进行现场验证,以探索减少化学品数量的可能性,特别是对于超集约化橄榄种植。本研究旨在对设计的立体篱篱作物静电喷雾器样机进行实验室评估,并通过田间试验验证其性能。与激活或停用静电系统(分别为ES和NES)相关的喷雾器配置进行了测试。表征包括气流评估、产品分布、电荷质量比(CMR)量化、沉积试验、不同的应用参数,如液体压力、气流设置和受控环境条件下的距离。在沉积测定期间,还进行了人工靶的正面和背面区域的区分。最后,通过田间试验将静电喷雾器与常规施用方法进行了比较,为农药减量效率提供了实际见解。实验室结果显示,在任何测试压力下,ES和NES配置之间的流量(L min - 1)没有差异。垂直分布剖面的总累积体积随气流结构和液体压力随距离的变化而变化。沉积结果显示,当静电系统被激活时,无论距离或高度如何,覆盖率和沉积量都更高,与NES相比,背面面积增加了70%。在超集约化橄榄果园进行的田间试验表明,静电设备在施用后,在人工受体和叶片上的覆盖和沉积方面表现良好,特别是在最高施用剂量(163.3 L ha - 1)下,与传统喷洒(466.3 L ha - 1)相比,突出表明施用量减少了60%。此外,静电喷雾器的接地损失显著降低,因为与传统设备相比,主干底部的覆盖和沉积大大减少。
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引用次数: 0
Contamination by systemic insecticide harms the dispersal ability of the lady beetle Hippodamia variegata 系统杀虫剂污染会损害斑姬瓢虫的扩散能力
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-09-23 DOI: 10.1016/j.cropro.2025.107426
Menghao Zhang , Hongchun Yv , Changchun Dai , Yanhui Lu
The lady beetle Hippodamia variegata (Coleoptera: Coccinellidae) is a generalist predator in Xinjiang, China, preying on aphids such as Aphis gossypii (Hemiptera: Aphididae) and other arthropods in agricultural ecosystems. Imidacloprid, the most widely used neonicotinoid insecticide globally, is commonly used to manage sucking pests such as aphids; however, it can have detrimental sublethal effects on some natural enemies. The development of IPM programs against pests requires an evaluation of the side effects of insecticides on important natural enemies. We found that exposure to aphids treated with sublethal levels of imidacloprid (LC20 and LC50) reduced longevity, fecundity, and flight performance of H. variegata adults. Specifically, exposure to aphids treated at the LC50 level reduced the flight distance and flight duration of the lady beetle by 81.9 % and 78.5 %, respectively, compared with the control group. Carbohydrate metabolism in lady beetles fed aphids in the LC20 and LC50 groups was significantly impaired, leading to higher trehalose and glycogen content compared to beetles in the control group that were fed untreated aphids. Moreover, the activity of metabolism enzymes was significantly reduced, although there was no significant effect on the levels of metabolism enzymes. In mark-release-recapture experiments conducted in cotton fields, sublethal concentrations of imidacloprid significantly reduced the dispersal distance of these beetles during the first two days after release. These results demonstrate that sublethal concentrations of imidacloprid have a negative impact on the flight dispersal ability of H. variegata, which could impair the regional biological control services provided by this predator.
斑姬瓢虫(鞘翅目:瓢虫科)是新疆农业生态系统中捕食棉蚜(半翅目:蚜虫科)等蚜虫和节肢动物的一种多面手捕食者。吡虫啉是全球使用最广泛的新烟碱类杀虫剂,通常用于防治蚜虫等吸吮性害虫;然而,它可以对一些天敌产生有害的亚致死效果。害虫综合防治计划的发展需要对杀虫剂对重要天敌的副作用进行评估。我们发现,暴露于亚致死水平的吡虫啉(LC20和LC50)处理的蚜虫会降低斑叶蚜成虫的寿命、繁殖力和飞行性能。与对照组相比,暴露于LC50水平的蚜虫使瓢虫的飞行距离和飞行时间分别减少了81.9%和78.5%。饲喂LC20和LC50蚜虫组的瓢虫碳水化合物代谢明显受损,导致海藻糖和糖原含量高于饲喂未处理蚜虫的对照组。此外,代谢酶活性显著降低,但对代谢酶水平无显著影响。在棉田进行的标记-释放-再捕获实验中,亚致死浓度的吡虫啉在释放后的头两天内显著减少了这些甲虫的扩散距离。上述结果表明,亚致死浓度的吡虫啉会对斑家鼠的飞行扩散能力产生负面影响,从而影响斑家鼠的区域生物防治功能。
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引用次数: 0
First report of Phyllosticta capitalensis causing black leaf spot on Camellia oleifera in Hunan, China 湖南油茶黑叶斑病发生初报
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-10-06 DOI: 10.1016/j.cropro.2025.107436
Zhaohui Hu , Liangxuan Qu , Xiaomao Zhou , Hong Liu , Guoying Zhou
Camellia oleifera is a multifunctional woody edible crop and is widely cultivated in China. Since 2024, black leaf spots were commonly observed on C. oleifera in Changde City and Zhuzhou City, Hunan Province, China. The pathogen was isolated from the symptomatic leaves and identified as Phyllosticta capitalensis based on morphological characteristics (colonies and conidia) as well as multi-locus phylogenetic analysis using the internal transcribed spacer region (ITS), translation elonation factor 1-alpha gene (tef1), and actin gene (act). Leaves inoculated with the representative isolates cause disease symptoms on C. oleifera leaves that are similar to those observed in the field. Koch's postulates were supported by pathogen re-isolation and identification from the inoculated leaves. As far as we know, this is the first report of P. capitalensis causing foliar disease on C. oleifera in Hunan Province, China. These findings will contribute to making scientific management strategies for this disease.
油茶是一种多功能木本食用作物,在中国广泛种植。自2024年以来,湖南常德市和株洲市的油桐普遍存在黑斑。通过形态学特征(菌落和分生孢子)和内部转录间隔区(ITS)、翻译扩增因子1- α基因(tef1)和肌动蛋白基因(act)的多位点系统发育分析,将病原菌从有症状的叶片中分离出来,鉴定为毛竹。用代表性分离株接种的叶片在油桐叶片上引起的疾病症状与田间观察到的症状相似。科赫的假设得到了从接种叶片中重新分离和鉴定病原体的支持。据我们所知,这是中国湖南省首次报道白顶霉引起油桐叶面病害的报道。这些发现将有助于制定科学的疾病管理策略。
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引用次数: 0
Fungicides for the management of blackleg disease of canola caused by Leptosphaeria maculans in the Western Cape province of South Africa 南非西开普省由斑点细螺旋体引起的油菜籽黑腿病的杀菌剂管理
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-09-16 DOI: 10.1016/j.cropro.2025.107404
Huibrecht Maria Schreuder , Gerhardus Johannes van Coller , Beatrix Coetzee , Diane Mostert
Blackleg disease, caused by Leptosphaeria maculans, severely impacts canola production in the Western Cape. Fungicides are an important component of integrated management, but little information is available on fungicide efficacy under South African conditions. With decreased sensitivity reported for L. maculans to the demethylation inhibitor (DMI) class fungicides in Australia and Europe, it is important that fungicide sensitivity is also evaluated locally. This study screened 118 L. maculans isolates, collected between 2007 and 2022 from canola growing regions of the Western Cape, for in vitro sensitivity to two active ingredients frequently used in canola production, namely tebuconazole and prothioconazole. Field trials were conducted at four locations in 2022 and 2023 to assess the efficacy of six fungicide products on two canola cultivars. Tebuconazole 50% effective concentrations (EC50) ranged from 144.9 ng/mL to 985.3 ng/mL, which was within the range of EC50 values found for sensitive isolates in similar studies. Tebuconazole EC50 values steadily increased from 2020 to 2022, suggesting a shift in sensitivity. Prothioconazole EC50 values were between 270.3 ng/mL and 1620.4 ng/mL. In the field trials, all fungicide products, except the unregistered product Bumper, were effective in lowering blackleg severity. The cultivar Diamond showed a greater fungicide response than the more resistant cultivar 44Y94. Under higher disease pressure, however, fungicide applications also reduced blackleg severity in 44Y94. When sequences of the CYP51 promotor region were screened for inserts associated with reduced DMI sensitivity, no inserts were detected in local isolates. This study provides a foundation for the effective and sustainable use of fungicides in an integrated blackleg management strategy in South Africa.
由斑点细螺旋体引起的黑腿病严重影响了西开普省的油菜籽生产。杀菌剂是综合管理的重要组成部分,但关于在南非条件下杀菌剂功效的资料很少。在澳大利亚和欧洲,有报道称L. maculans对去甲基化抑制剂(DMI)类杀菌剂的敏感性降低,因此对当地杀菌剂的敏感性进行评估是很重要的。本研究筛选了2007年至2022年间从西开普省油菜籽种植区收集的118株maculans分离株,对油菜籽生产中常用的两种活性成分(即戊康唑和原硫康唑)进行了体外敏感性测试。2022年和2023年在4个地点进行了田间试验,以评估6种杀菌剂产品对两种油菜品种的药效。戊康唑50%有效浓度(EC50)范围为144.9 ~ 985.3 ng/mL,在类似研究中敏感分离株的EC50值范围内。从2020年到2022年,戊康唑的EC50值稳步上升,表明敏感性发生了变化。Prothioconazole的EC50值在270.3 ~ 1620.4 ng/mL之间。在田间试验中,除未注册产品Bumper外,所有杀菌剂均能有效降低黑腿的严重程度。品种Diamond对杀菌剂的反应强于抗病品种44Y94。然而,在较高的疾病压力下,施用杀菌剂也降低了44Y94的黑腿严重程度。当筛选CYP51启动子区域序列中与DMI敏感性降低相关的插入物时,在本地分离株中未检测到插入物。本研究为在南非黑腿综合管理战略中有效和可持续地使用杀菌剂提供了基础。
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引用次数: 0
Antifeedant activities of some plant secondary metabolites on cotton leafworm (Spodoptera littoralis (Boisduval, 1833)) (Lepidoptera: Noctuidae) 几种植物次生代谢物对棉花叶虫(Spodoptera littoralis (Boisduval, 1833))的拒食活性(鳞翅目:夜蛾科)
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-09-11 DOI: 10.1016/j.cropro.2025.107401
Zekeriya Köker , Ali Arda Işıkber
This study evaluated the antifeedant activities of selected plant secondary metabolites (PSMs)—allyl isothiocyanate, geraniol, allyl disulfide, eugenol, citronellal, carvacrol, tannin, gossypol, α-pinene, and carvone— against fourth-instar larvae of the cotton leafworm (Spodoptera littoralis (Boisduval, 1833)) under controlled laboratory conditions. These compounds were incorporated into artificial diets at 10, 100, and 1000 ppm concentrations, and their effects were assessed using both choice and no-choice feeding tests. The no-choice feeding tests showed that allyl isothiocyanate, geraniol, allyl disulfide, and carvacrol exhibited stronger antifeedant activity compared to the other PSMs. The antifeedant index (AFI) values of allyl isothiocyanate, eugenol, carvacrol, α-pinene, and carvone increased with higher concentrations whereas the AFI values of citronellal, geraniol, tannin, and gossypol showed no significant change across concentrations. All tested PSMs demonstrated positive efficiency of conversion of ingested food (ECI) values at 10 and 100 ppm, indicating efficient nutrient utilization at lower doses. However, at 1000 ppm, allyl isothiocyanate, allyl disulfide, carvacrol, and carvone caused negative ECI values, suggesting impaired assimilation and potential toxicity. In choice feeding tests, carvacrol, allyl isothiocyanate, and allyl disulfide consistently exhibited significantly higher AFI values across all feeding periods at 10 and 100 ppm, outperforming the remaining PSMs. Among them, carvacrol displayed the highest and most stable AFI values across all concentrations and feeding times. In conclusion, both bioassay approaches consistently identified allyl isothiocyanate, allyl disulfide, and carvacrol as the most potent antifeedants against S. littoralis larvae, suggesting their potential for further evaluation as botanical deterrents in integrated pest management programs.
本研究在实验室条件下评价了植物次生代谢物(psm)——异硫氰酸烯丙酯、香叶醇、二硫烯丙酯、丁香酚、香茅醛、香芹醇、单宁、棉酚、α-蒎烯和香芹酮对棉花叶虫(Spodoptera littoralis (Boisduval, 1833)) 4龄幼虫的反食活性。将这些化合物以10、100和1000 ppm的浓度加入人工饲料中,并通过选择和不选择饲养试验评估其效果。无选择饲喂试验表明,异硫氰酸烯丙酯、香叶醇、二硫化烯丙酯和香芹酚具有较强的拒食活性。异硫氰酸烯丙酯、丁香酚、香芹酚、α-蒎烯和香芹酮的拒食指数随浓度升高而升高,香茅醛、香叶醇、单宁和棉酚的拒食指数在不同浓度下变化不显著。所有测试的psm在10和100 ppm时均显示出摄入食物(ECI)值的正转换效率,表明在较低剂量下有效利用营养物质。然而,在1000ppm时,异硫氰酸烯丙酯、二硫烯丙酯、香芹酚和香芹酮的ECI值为负,表明同化受损和潜在毒性。在选择性饲喂试验中,香芹酚、异硫氰酸烯丙酯和二硫化烯丙酯在10 ppm和100 ppm的饲喂条件下始终表现出显著较高的AFI值,优于其他psm。其中,香芹酚在各浓度和饲喂时间的AFI值最高且最稳定。总之,两种生物测定方法一致鉴定出异硫氰酸烯丙酯、二硫化物烯丙酯和香芹酚是对滨海夜蛾幼虫最有效的抗食剂,这表明它们在害虫综合治理计划中作为植物威慑物的潜力有待进一步评估。
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引用次数: 0
Erioglossum rubiginosum, a new alternative host of rubber tree powdery mildew Erysiphe quercicola 橡胶树白粉病的一种新的替代寄主
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-10-11 DOI: 10.1016/j.cropro.2025.107443
Zheyu Zhang , Shanshan Yin , Jonathan S. West , Daquan Luo , Xueren Cao
Erioglossum rubiginosum (synonym as Lepisanthes rubiginosa), is a shrub-like plant belonging to the family Sapindaceae. This species is a common undergrowth plant species in rubber tree plantations, which provide more than 90% of the total natural rubber production. Powdery mildew was found to occur seriously on E. rubiginosum during an investigation on powdery mildew of rubber tree caused by Erysiphe quercicola. In this study, leaves of E. rubiginosum with powdery mildew symptoms were collected and the pathogen was identified using morphological and molecular analyses using the internal transcribed spacer (ITS) and 28 S rDNA regions. The results indicated that E. quercicola was the causal agent of E. rubiginosum powdery mildew. Based on cross-pathogenicity analysis, E. quercicola from E. rubiginosum and rubber tree could cause typical symptoms on each other, which confirmed that E. rubiginosum is an alternative host of rubber tree powdery mildew. To our knowledge, this is the first report of E. quercicola causing powdery mildew on E. rubiginosum. Whether E. rubiginosum can be one of the primary sources of the rubber tree powdery mildew epidemics needs future studies.
红毛藓(Lepisanthes rubiginosa)是一种灌木状植物,属于皂科。本种是橡胶树人工林中常见的林下植物,占天然橡胶总产量的90%以上。通过对橡胶树白粉病的调查,发现橡胶树白粉病发生严重。本研究收集了具有白粉病症状的红荷叶,利用其内部转录间隔区(ITS)和28个S rDNA区进行形态学和分子鉴定。结果表明,槲寄生菌是导致褐皮杆菌白粉病的病原菌。交叉致病性分析表明,橡胶树的槲寄生菌与橡胶树的槲寄生菌可相互引起典型症状,证实了橡胶树的槲寄生菌是橡胶树白粉病的替代寄主。据我们所知,这是第一次报道槲皮蚧引起红毛螨白粉病。橡胶树白粉病是否可能是橡胶树白粉病流行的主要来源之一还有待进一步研究。
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引用次数: 0
Stability analyses of charcoal rot resistance and yield-related traits in post-rainy sorghum genotypes using AMMI and MTSI in multi-environment trials 多环境试验中利用AMMI和MTSI分析雨后高粱基因型抗炭腐及产量相关性状的稳定性
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-09-30 DOI: 10.1016/j.cropro.2025.107427
I.K. Das , R. Madhusudhana , Baswaraj R , V.M. Gholve , M.R. Govindappa , N. Kannababu , G. Girish
Charcoal rot (CR), caused by Macrophomina phaseolina, is the most economically significant soil-borne disease of sorghum in many countries. The disease is highly influenced by the environment and known to be severe in high-yielding cultivars. Therefore, developing a CR resistant cultivar combined with high yield is challenging. Information on the relationship between traits associated with CR resistance and yield is lacking. This multi-environment study analysed genetic variation and relationships among the major traits associated with CR resistance—namely, CR index (CRI), CR lesion length (LL), and lodging (LOG); and yield—namely, grain yield (GY), stover yield (SY), and harvest index (HI) in popular post-rainy (Rabi) sorghum cultivars in India. The objective was to identify cultivar(s) that balance(s) stability and performance for both CR resistance and yield traits for pan-India presence. Eight cultivars were evaluated in replicated field trials across eight environments in India during the post-rainy seasons of 2018–2020. Most of the traits showed significant positive correlations with each other (p < 0.05 to p < 0.001). Environment-wise individual analysis of variance indicated significant variations for all traits in nearly all environments. The additive main effects and multiplicative interaction (AMMI) analysis of variance revealed highly significant (p < 0.001) genotypic (G), environmental (E), and G × E interaction effects for all the traits. Based on performance on mean and stability, the top two genotypes in AMMI analysis were CSV26 and CSV29R. Analysis using the multi-trait stability index (MTSI) identified the cultivar CSV26 for combined performance on mean and stability, as well as stability alone, whereas CSV29R was identified for performance on mean alone. Among the eight tested cultivars, CSV26 was identified as the best genotype for performance on mean and stability in AMMI analysis, multi-trait stability index (MTSI) analysis, and based on predicted mean values in best linear unbiased prediction (BLUP). CSV26 will serve as an important source material for developing stable sorghum cultivars for pan-India performance. The simultaneous use of data analysis methods, namely AMMI, MTSI, and BLUP, would be highly beneficial for plant breeders, enhancing the precision of selecting superior genotypes from multi-environment trials.
木炭腐病(CR)是许多国家高粱土传病害中最具经济意义的一种,由phaseolina Macrophomina引起。该病受环境影响很大,已知在高产品种中较为严重。因此,培育抗CR的高产品种具有一定的挑战性。目前缺乏与CR抗性相关性状与产量之间关系的信息。这项多环境研究分析了与CR抗性相关的主要性状,即CR指数(CRI)、CR病变长度(LL)和倒伏(LOG)之间的遗传变异和关系;和产量,即粮食产量(GY),秸秆产量(SY)和收获指数(HI)在印度流行的雨后高粱品种。目标是确定在泛印度存在的CR抗性和产量性状方面平衡稳定性和性能的品种。2018-2020年雨季结束后,在印度8个环境中对8个品种进行了重复田间试验。大部分性状之间呈显著正相关(p < 0.05 ~ p < 0.001)。环境方面的个体方差分析表明,在几乎所有环境中,所有性状都存在显著差异。加性主效应和乘性互作(AMMI)方差分析显示,所有性状的基因型(G)、环境(E)和G × E互作效应均极显著(p < 0.001)。根据平均性能和稳定性,AMMI分析中排名前两位的基因型为CSV26和CSV29R。利用多性状稳定指数(MTSI)对CSV26和CSV29R分别进行了平均性能和稳定性综合及稳定性单性状分析。在8个被试品种中,CSV26在AMMI分析、多性状稳定指数(MTSI)分析和最佳线性无偏预测(BLUP)预测均值方面均表现最佳。CSV26将成为培育具有泛印度性能的稳定高粱品种的重要原料。同时使用AMMI、MTSI和BLUP等数据分析方法,可以提高植物育种者从多环境试验中选择优良基因型的精度。
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引用次数: 0
Plant volatiles from Mentha haplocalyx repel Grapholita molesta and decrease the infestation rate of apple fruits 薄荷挥发物对苹果果品有驱虫作用,可降低苹果果品侵染率
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-10-02 DOI: 10.1016/j.cropro.2025.107430
Yunhe Zhang , Fan Wang , Xiaotong Zhou , Wenhui Zhang , Ziyu Hu , Hengqiu Jia , Zhaotan Li , Xiaoxiao Zhang , Donghai Zhang , Zhiping Cai
Grapholita molesta is a globally prevalent fruit-boring pest characterized by its overlapping generations, resulting in annual economic losses. Mentha haplocalyx, as a functional plant, exhibits effective pest-repelling properties against a range of pests. To date, no studies have investigated the repellent effects of M. haplocalyx on G. molesta. through both field and laboratory investigations, M. haplocalyx reduced G. molesta fruit infestation from 33.00 % to 9.67 % in the field and from 77.78 % to 56.67 % in the laboratory. In the behavioral response experiment, the repellency rate of M. haplocalyx against 1-day-old unmated male and female G. molesta adults exceeded 80 %. Eight and five compounds that elicited electroantennographic detection responses in female and male G. molesta adults, respectively, were identified through gas chromatography-mass spectrometry. The electroantennogram responses revealed that all of the tested active compounds elicited antennal sensitivity. The olfactory behavioral response experiments revealed that among the 8 electroantennographic detection active compounds tested in female adults, 6-methylhept-5-en-2-one (0.1, 1, and 10 μL/mL), ether (0.1 μL/mL), 1,1,2,2-tetrachloroethane (0.1 and 1 μL/mL), ethyl 3-methyl-2-butenoate (1 μL/mL), decanal (1 μL/mL), p-xylene (1 and 10 μL/mL), and octamethylcyclotetrasiloxane (10 μL/mL) exhibited significant repellent effects on female G. molesta adults, but decamethylcyclopentasiloxane did not demonstrate tropism at all of the tested concentrations. In male adults, ethyl isovalerate (0.1, 1, and 10 μL/mL), benzoylthiocarbimide (0.1 μL/mL), ethyl carbonate (0.1 and 1 μL/mL), nonanal (0.1 and 1 μL/mL), and 6-methylhept-5-en-2-one (1 and 10 μL/mL) showed significant repellent effects. This study proposes an innovative intercropping strategy that can effectively suppress G. molesta, thereby providing valuable insights for sustainable and eco-friendly pest management in agriculture.
小蠹蛾是一种全球普遍存在的蛀果害虫,其特点是世代重叠,每年造成经济损失。薄荷作为一种功能性植物,对多种害虫具有有效的驱虫特性。到目前为止,还没有研究调查了单叶蝉对大叶蝉的驱避作用。通过田间和室内调查,单叶蝉的田间侵染率由33.00%降至9.67%,室内侵染率由77.78%降至56.67%。在行为反应实验中,单斑姬螨对1日龄未交配雌雄大鼠成虫的驱避率超过80%。通过气相色谱-质谱联用技术,分别鉴定了8种和5种能在雌鼠和雄鼠成虫中引起触角电位检测反应的化合物。天线图反应显示,所有测试的活性化合物都引起了天线敏感性。嗅觉行为反应实验表明,在雌性成虫的触角电检测活性化合物中,6-甲基庚-5-烯-2-酮(0.1、1和10 μL/mL)、醚(0.1 μL/mL)、1,1,2,2-四氯乙烷(0.1和1 μL/mL)、3-甲基-2-丁烯酸乙酯(1 μL/mL)、癸醛(1 μL/mL)、对二甲苯(1和10 μL/mL)和八甲基环四硅氧烷(10 μL/mL)对雌性成虫具有显著的驱避作用;但在所有测试浓度下,十甲基环五硅氧烷均未表现出向性。在雄性成虫中,异戊酸乙酯(0.1、1和10 μL/mL)、苯甲酰硫脲(0.1 μL/mL)、碳酸乙酯(0.1和1 μL/mL)、壬醛(0.1和1 μL/mL)和6-甲基庚-5-烯-2-酮(1和10 μL/mL)的驱避效果显著。本研究提出了一种创新的间作策略,可以有效地抑制害虫,从而为农业害虫的可持续和生态管理提供有价值的见解。
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引用次数: 0
Virtual IAPPS Governing Board meeting – Sept 5, 2025 虚拟IAPPS管理委员会会议- 2025年9月5日
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-10-04 DOI: 10.1016/j.cropro.2025.107428
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引用次数: 0
Evidence of Pyrethroid Resistance in the bruchid beetle, Bruchus rufimanus, in Ireland 爱尔兰Bruchus rufimanus对拟除虫菊酯抗性的证据
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2026-01-01 Epub Date: 2025-09-20 DOI: 10.1016/j.cropro.2025.107407
Antoine Pichon , Stephen Byrne , Sheila Alves , Eamon Nolan , Louise McNamara
Bruchus rufimanus Boh. (Coleoptera; Chrysomelidae) is a significant pest of Vicia faba L. (faba bean), adversely affecting yield and grain quality. The primary control method currently employed against this pest involves the application of a pyrethroid insecticide when the initial bean pods emerge in the field and the temperature reaches 20 °C for two consecutive days. However, potential resistance to pyrethroids has been observed in B. rufimanus populations in Europe. This study provides substantial evidence for resistance to a pyrethroid λ-cyhalothrin in an Irish population of B. rufimanus. The results demonstrate resistance with an LD50 of 114 % compared to the maximum individual dose allowed of 7.5g a.i./ha. The mechanism of resistance involves metabolic resistance, as evidenced by an increase in the efficacy of λ-cyhalothrin when combined with Piperonyl Butoxide (PBO), a synergist that inhibits detoxifying enzymes. Additionally, the population was examined for the L1014F mutation in the VGSC gene, which is responsible for most cases of pyrethroid resistance in Chrysomelidae, and no L1014F mutation was identified. Our results describe for the first time the presence of pyrethroid resistance in B. rufimanus in Ireland. This work emphasises the difficulties in controlling B. rufimanus with insecticide and highlights the requirement to study the evolution of resistance in populations to provide an appropriate management strategy to growers.
Bruchus rufimanus。蚕豆蛾(Coleoptera; Chrysomelidae)是蚕豆的重要害虫,严重影响产量和籽粒品质。目前对这种害虫采用的主要防治方法是在田间初出豆荚并且连续两天温度达到20°C时施用拟除虫菊酯类杀虫剂。然而,在欧洲已观察到对拟除虫菊酯类杀虫剂的潜在抗性。本研究为爱尔兰褐毛小蠊种群对拟除虫菊酯类杀虫剂λ-氯氟氰菊酯的抗性提供了有力证据。结果表明,与允许的最大个体剂量7.5g a.i./ha相比,LD50为114%。抗性机制涉及代谢性抗性,如λ-氯氟氰菊酯与抑制解毒酶的增效剂胡椒酰丁醇(PBO)联合使用时,其功效增加。此外,对该种群进行了VGSC基因的L1014F突变检测,该基因是导致金曲蝇对拟除虫菊酯抗性的主要原因,但未发现L1014F突变。我们的研究结果首次描述了在爱尔兰出现的拟除虫菊酯抗性。这项工作强调了用杀虫剂控制红毛小蠊的困难,并强调了研究种群抗性进化的必要性,以便为种植者提供适当的管理策略。
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引用次数: 0
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Crop Protection
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