Pub Date : 2024-12-16DOI: 10.1016/j.cropro.2024.107089
Yu-Ru Wang, Bo-Ying Huang, Hafiz Muhammad Usman, Khadija Javed, Fatimah Al-Otibi, Kevin D. Hyde, Yong Wang
Plums (Prunus salicina) are highly valued for their diverse flavors and nutritional benefits, making them essential ingredients in processed foods such as juices and jams, making them one of the world's most popular fruits. However, their postharvest short shelf life, compounded by rapid deterioration and rot, presents significant challenges to maintaining fruit quality and extending shelf stability, resulting in substantial economic losses for the industry. This study employed both morphological and molecular biology approaches to identify and characterize pathogens from diseased plum samples collected in major production regions. Key pathogens responsible for postharvest fruit rot in plums in Guizhou, China, were identified as Botryosphaeria wangensis, Colletotrichum nymphaeae, Diaporthe eres, and Geotrichum candidum. The comprehensive identification of these causative agents is crucial for developing targeted disease management strategies. This research contributes crucial insights for enhancing plum quality and extending shelf stability in the industry, addressing essential needs in fruit preservation and economic sustainability.
{"title":"First report of Botryosphaeria wangensis, Colletotrichum nymphaeae, Diaporthe eres, and Geotrichum candidum causing postharvest fruit rot of plums (Prunus salicina) in China","authors":"Yu-Ru Wang, Bo-Ying Huang, Hafiz Muhammad Usman, Khadija Javed, Fatimah Al-Otibi, Kevin D. Hyde, Yong Wang","doi":"10.1016/j.cropro.2024.107089","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107089","url":null,"abstract":"Plums (<ce:italic>Prunus salicina</ce:italic>) are highly valued for their diverse flavors and nutritional benefits, making them essential ingredients in processed foods such as juices and jams, making them one of the world's most popular fruits. However, their postharvest short shelf life, compounded by rapid deterioration and rot, presents significant challenges to maintaining fruit quality and extending shelf stability, resulting in substantial economic losses for the industry. This study employed both morphological and molecular biology approaches to identify and characterize pathogens from diseased plum samples collected in major production regions. Key pathogens responsible for postharvest fruit rot in plums in Guizhou, China, were identified as <ce:italic>Botryosphaeria wangensis</ce:italic>, <ce:italic>Colletotrichum nymphaeae</ce:italic>, <ce:italic>Diaporthe eres</ce:italic>, and <ce:italic>Geotrichum candidum</ce:italic>. The comprehensive identification of these causative agents is crucial for developing targeted disease management strategies. This research contributes crucial insights for enhancing plum quality and extending shelf stability in the industry, addressing essential needs in fruit preservation and economic sustainability.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"12 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The sweet potato whitefly, Bemisia tabaci (Gennadius) is one of the most devastating pests, inflicting severe damage on a wide range of crops. The tetramic acid insecticides, spirotetramat and spiropidion, act as inhibitors of lipid biosynthesis by targeting acetyl-coenzyme A carboxylase (ACCase), disrupting fatty acid biosynthesis and energy metabolism. In the present study, a total of 47 field populations were collected across China in 2021 and 2022, and their susceptibilities to spirotetramat and spiropidion were determined in the laboratory. The results showed that in contrast to their toxicities against nymphs, spirotetramat and spiropidion exhibited minimal toxicity against B. tabaci adults. B. tabaci nymphs from field populations exhibited susceptibility or low resistance to spirotetramat, with LC50 values ranging from 2.85 to 13.58 mg L−1 and resistance ratio (RR) from 1.7 to 8.2. There was a variation in the sensitivity of B. tabaci field populations towards spiropidion, with LC50 values ranging from 13.12 to 120.13 mg L−1 and RR from 3.5 to 23.4. The baseline susceptibility of B. tabaci nymphs to spiropidion was determined to be 24.06 mg L⁻1, corresponding to the median lethal concentration (LC50) calculated from 43 field populations. Cross-resistance was observed between spirotetramat and spiropidion, as well as between cyantraniliprole and tetramic acid insecticides. However, no significant cross-resistance was found between neonicotinoids and tetramic acid insecticides. Collectively, these findings improve our knowledge on the toxicity of tetramic acid insecticides to B. tabaci populations in China and provide valuable information for their scientific application in the field.
{"title":"Sensitivity monitoring of Bemisia tabaci to two tetramic acid insecticides and baseline susceptibility to spiropidion in China","authors":"Xiaolan Liu, Xichao Hu, Zanrong Wen, Haoyu Tang, Jianyu Ma, Bo Qiu, Wei Xu, Xiuli Chi, Chao Lv, Lei Guo, Yongjun Zhang","doi":"10.1016/j.cropro.2024.107088","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107088","url":null,"abstract":"The sweet potato whitefly, <ce:italic>Bemisia tabaci</ce:italic> (Gennadius) is one of the most devastating pests, inflicting severe damage on a wide range of crops. The tetramic acid insecticides, spirotetramat and spiropidion, act as inhibitors of lipid biosynthesis by targeting acetyl-coenzyme A carboxylase (ACCase), disrupting fatty acid biosynthesis and energy metabolism. In the present study, a total of 47 field populations were collected across China in 2021 and 2022, and their susceptibilities to spirotetramat and spiropidion were determined in the laboratory. The results showed that in contrast to their toxicities against nymphs, spirotetramat and spiropidion exhibited minimal toxicity against <ce:italic>B. tabaci</ce:italic> adults. <ce:italic>B. tabaci</ce:italic> nymphs from field populations exhibited susceptibility or low resistance to spirotetramat, with LC<ce:inf loc=\"post\">50</ce:inf> values ranging from 2.85 to 13.58 mg L<ce:sup loc=\"post\">−1</ce:sup> and resistance ratio (RR) from 1.7 to 8.2. There was a variation in the sensitivity of <ce:italic>B. tabaci</ce:italic> field populations towards spiropidion, with LC<ce:inf loc=\"post\">50</ce:inf> values ranging from 13.12 to 120.13 mg L<ce:sup loc=\"post\">−1</ce:sup> and RR from 3.5 to 23.4. The baseline susceptibility of <ce:italic>B. tabaci</ce:italic> nymphs to spiropidion was determined to be 24.06 mg L⁻<ce:sup loc=\"post\">1</ce:sup>, corresponding to the median lethal concentration (LC<ce:inf loc=\"post\">50</ce:inf>) calculated from 43 field populations. Cross-resistance was observed between spirotetramat and spiropidion, as well as between cyantraniliprole and tetramic acid insecticides. However, no significant cross-resistance was found between neonicotinoids and tetramic acid insecticides. Collectively, these findings improve our knowledge on the toxicity of tetramic acid insecticides to <ce:italic>B. tabaci</ce:italic> populations in China and provide valuable information for their scientific application in the field.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"132 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-15DOI: 10.1016/j.cropro.2024.107081
Shuqi Yang, Yue Yuan, Xiongwei Zhang, Aoju Feng, Yaqin Zou, Pan Yao, Danni Ye, Kaiyi Zeng, Xiaolei Zhang, Zhihong Xu
<ce:italic>Spodoptera frugiperda</ce:italic> (J.E. Smith) is a major insect pest affecting corn globally. <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> often coincides with maize diseases, as their damage periods overlap. Consequently, <ce:italic>S. frugiperda</ce:italic> may be exposed to varying types and quantities of these fungicides during the growing season. The aim of this study was to determine the effects of difenoconazole and pyraclostrobin on the biological and physiological traits of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Treatment with difenoconazole and pyraclostrobin significantly prolonged the pupal development period in the F<ce:inf loc="post">0</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> compared to the control group. Additionally, exposure to difenoconazole shortened the development periods of the 1st and 6th instar larvae and reduced the adult preoviposition period (APOP) in the F<ce:inf loc="post">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>. However, it extended the development period of the 4th instar larvae and pupae, as well as overall longevity. The pyraclostrobin treatment group exhibited a shorter development period of 2nd instar larvae, as well as longer longevity, and more eggs/per female in the F<ce:inf loc="post">1</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Compared to the control group, the fecundity of the offspring of the pyraclostrobin treatment group increased by 68.24%. Furthermore, treatment with difenoconazole and pyraclostrobin significantly increased the intrinsic rate of increase (<ce:italic>r</ce:italic>) and the finite rate of increase (<ce:italic>λ</ce:italic>) in the F<ce:inf loc="post">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>, while significantly reducing the mean generation time (<ce:italic>T</ce:italic>). Additionally, mRNA expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP321A10</ce:italic>, <ce:italic>CYP341A11</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13059</ce:italic>, <ce:italic>CXE23</ce:italic>, <ce:italic>ABC01998, ABC18240</ce:italic>, and <ce:italic>UGT17742</ce:italic> were significantly elevated in F<ce:inf loc="post">0</ce:inf> individuals from larvae treated with difenoconazole. Meanwhile, the relative expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP314A1</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13095</ce:italic>, <ce:italic>CXE20</ce:italic>, <ce:italic>ABC01998</ce:italic>, <ce:italic>ABC18240</ce:italic>, <ce:italic>ABC22158</ce:italic>, and <ce:italic>ABC22158</ce:italic> in the larvae (F<ce:inf loc="post">0</ce:inf>) exposed to pyraclostrobin were also elevated. Compared to the control and difenoconazole treatment groups, the expression of
{"title":"Effects of difenoconazole and pyraclostrobin on the development, reproduction, and detoxifying metabolism of Spodoptera frugiperda (J.E. Smith)","authors":"Shuqi Yang, Yue Yuan, Xiongwei Zhang, Aoju Feng, Yaqin Zou, Pan Yao, Danni Ye, Kaiyi Zeng, Xiaolei Zhang, Zhihong Xu","doi":"10.1016/j.cropro.2024.107081","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107081","url":null,"abstract":"<ce:italic>Spodoptera frugiperda</ce:italic> (J.E. Smith) is a major insect pest affecting corn globally. <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> often coincides with maize diseases, as their damage periods overlap. Consequently, <ce:italic>S. frugiperda</ce:italic> may be exposed to varying types and quantities of these fungicides during the growing season. The aim of this study was to determine the effects of difenoconazole and pyraclostrobin on the biological and physiological traits of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Treatment with difenoconazole and pyraclostrobin significantly prolonged the pupal development period in the F<ce:inf loc=\"post\">0</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> compared to the control group. Additionally, exposure to difenoconazole shortened the development periods of the 1st and 6th instar larvae and reduced the adult preoviposition period (APOP) in the F<ce:inf loc=\"post\">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>. However, it extended the development period of the 4th instar larvae and pupae, as well as overall longevity. The pyraclostrobin treatment group exhibited a shorter development period of 2nd instar larvae, as well as longer longevity, and more eggs/per female in the F<ce:inf loc=\"post\">1</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Compared to the control group, the fecundity of the offspring of the pyraclostrobin treatment group increased by 68.24%. Furthermore, treatment with difenoconazole and pyraclostrobin significantly increased the intrinsic rate of increase (<ce:italic>r</ce:italic>) and the finite rate of increase (<ce:italic>λ</ce:italic>) in the F<ce:inf loc=\"post\">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>, while significantly reducing the mean generation time (<ce:italic>T</ce:italic>). Additionally, mRNA expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP321A10</ce:italic>, <ce:italic>CYP341A11</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13059</ce:italic>, <ce:italic>CXE23</ce:italic>, <ce:italic>ABC01998, ABC18240</ce:italic>, and <ce:italic>UGT17742</ce:italic> were significantly elevated in F<ce:inf loc=\"post\">0</ce:inf> individuals from larvae treated with difenoconazole. Meanwhile, the relative expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP314A1</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13095</ce:italic>, <ce:italic>CXE20</ce:italic>, <ce:italic>ABC01998</ce:italic>, <ce:italic>ABC18240</ce:italic>, <ce:italic>ABC22158</ce:italic>, and <ce:italic>ABC22158</ce:italic> in the larvae (F<ce:inf loc=\"post\">0</ce:inf>) exposed to pyraclostrobin were also elevated. Compared to the control and difenoconazole treatment groups, the expression of ","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"90 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-15DOI: 10.1016/j.cropro.2024.107079
Yao Huang, Xiuqin Wang, Houding Ou, Lan Wei, Xiaofei Yu, Bo Wang, Yi Mao, Maofa Yang
In the three-level nutritional structure of plant–phytophagous insect–natural enemies, plants exert direct and indirect effects on natural enemies. Additionally, the effect of the host diet on natural enemies also has an indirect impact on pest control. Herein, the effects of two diets of Ephestia elutella on the fitness of its natural enemy Habrobracon hebetor were studied using an age–stage, two-sex life table analysis. The results indicated that the total fecundity, the number of eggs laid during each oviposition day (Ed), oviposition days, and female longevity of the H. hebetor of F0 generation parasitized on E. elutella fed an artificial diet were significantly higher than those on E. elutella fed tobacco leaves. The pre-adult survival, paralysis, and parasitism rates of the H. hebetor F1 generation parasitized on E. elutella fed an artificial diet were also significantly higher than those on E. elutella fed tobacco leaves. The results provide a theoretical basis for the large-scale propagation of H. hebetor in the laboratory and for improving the efficiency of H. hebetor in controlling E. elutella in tobacco warehouses.
{"title":"Effect of Ephestia elutella diets on fitness of the third trophic level, the parasitoid Habrobracon hebetor (Hymenoptera: Braconidae)","authors":"Yao Huang, Xiuqin Wang, Houding Ou, Lan Wei, Xiaofei Yu, Bo Wang, Yi Mao, Maofa Yang","doi":"10.1016/j.cropro.2024.107079","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107079","url":null,"abstract":"In the three-level nutritional structure of plant–phytophagous insect–natural enemies, plants exert direct and indirect effects on natural enemies. Additionally, the effect of the host diet on natural enemies also has an indirect impact on pest control. Herein, the effects of two diets of <ce:italic>Ephestia elutella</ce:italic> on the fitness of its natural enemy <ce:italic>Habrobracon hebetor</ce:italic> were studied using an age–stage, two-sex life table analysis. The results indicated that the total fecundity, the number of eggs laid during each oviposition day (<ce:italic>Ed</ce:italic>), oviposition days, and female longevity of the <ce:italic>H. hebetor</ce:italic> of F<ce:inf loc=\"post\">0</ce:inf> generation parasitized on <ce:italic>E. elutella</ce:italic> fed an artificial diet were significantly higher than those on <ce:italic>E. elutella</ce:italic> fed tobacco leaves. The pre-adult survival, paralysis, and parasitism rates of the <ce:italic>H. hebetor</ce:italic> F<ce:inf loc=\"post\">1</ce:inf> generation parasitized on <ce:italic>E. elutella</ce:italic> fed an artificial diet were also significantly higher than those on <ce:italic>E. elutella</ce:italic> fed tobacco leaves. The results provide a theoretical basis for the large-scale propagation of <ce:italic>H. hebetor</ce:italic> in the laboratory and for improving the efficiency of <ce:italic>H. hebetor</ce:italic> in controlling <ce:italic>E. elutella</ce:italic> in tobacco warehouses.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"41 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Durian (Durio zibethinus L.) is a valuable fruit crop originating from Borneo and widely cultivated in Southeast Asia, including Malaysia, Thailand, and Indonesia. Increasing consumer demand, particularly from China, has boosted its trade and value. However, the durian industry faces significant challenges from the devastating pathogens Phytophthora palmivora and Phytopythium vexans, which cause various forms of rot and dieback, leading to economic losses. Current management strategies include cultural practices, synthetic fungicides, biological controls, and integrated management. While cultural practices and synthetic fungicides are commonly used, they have limitations such as health and environmental risks, labor intensity, and inconsistent effectiveness. Alternative approaches, such as gene silencing of Protein Phosphatase 2A to produce disease-resistant plants and the use of plant extracts with antimicrobial properties, show promise and warrant further exploration. This review aims to guide farmers and beginners in exploring alternative methods for managing durian diseases.
{"title":"Management of Phytophthora and Phytopythium oomycete diseases in durian (Duriozibethinus)","authors":"Ajit Singh, Caryn Chow, Kevin Nathaniel, Yap Lip Vun, Sumera Javad, Khajista Jabeen","doi":"10.1016/j.cropro.2024.107086","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107086","url":null,"abstract":"Durian (<ce:italic>Durio zibethinus</ce:italic> L.) is a valuable fruit crop originating from Borneo and widely cultivated in Southeast Asia, including Malaysia, Thailand, and Indonesia. Increasing consumer demand, particularly from China, has boosted its trade and value. However, the durian industry faces significant challenges from the devastating pathogens <ce:italic>Phytophthora palmivora</ce:italic> and <ce:italic>Phytopythium vexans</ce:italic>, which cause various forms of rot and dieback, leading to economic losses. Current management strategies include cultural practices, synthetic fungicides, biological controls, and integrated management. While cultural practices and synthetic fungicides are commonly used, they have limitations such as health and environmental risks, labor intensity, and inconsistent effectiveness. Alternative approaches, such as gene silencing of Protein Phosphatase 2A to produce disease-resistant plants and the use of plant extracts with antimicrobial properties, show promise and warrant further exploration. This review aims to guide farmers and beginners in exploring alternative methods for managing durian diseases.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"17 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-15DOI: 10.1016/j.cropro.2024.107085
Alexandra Díez-Méndez, Isabel García-Izquierdo, Jorge Poveda
The escalating impacts of climate change are exacerbating the spread and intensity of Botrytis bunch rot (BBR), a devastating grapevine disease caused by the necrotrophic fungus Botrytis cinerea. Growers have traditionally relied on synthetic fungicides to manage BBR, but the increasing resistance of B. cinerea to these chemicals, coupled with growing environmental concerns, has intensified the search for more sustainable and eco-friendly control strategies. Microbial biological control agents (MBCAs) have emerged as a promising alternative for BBR management. This review explores research on using MBCAs to control BBR in grapevine fields. Various microorganisms have shown efficacy, including bacteria such as Bacillus subtilis, B. amyloliquefaciens, B. ginsengihumi, Pantoea agglomerans or Pseudomonas fluorescens, yeasts such as Saccharomyces cerevisiae, Metschnikowia fructicola or Candida sake, and filamentous fungi such as Trichoderma asperellum, T. pseudokoningii, Fusarium solani or Ulocladium atrum. These MBCAs employ diverse mechanisms of action, including direct antagonism through competition for space and nutrients, the production of lytic enzymes that degrade fungal cell walls, and the induction of plant defense mechanisms at both local and systemic levels. However, the effectiveness of these MBCAs can be influenced by environmental factors, with high temperatures and low humidity generally favoring their activity. Further research is needed to optimize MBCA application methods, elucidate the complex interactions between MBCAs, the pathogen, and the grapevine host, and explore the potential of combining multiple MBCAs for enhanced disease control. Integrating MBCAs with other sustainable practices offers a more holistic approach to BBR management, paving the way for a more environmentally friendly and effective strategy to combat this devastating disease.
{"title":"Microbial biological control of Botrytis bunch rot (Botrytis cinerea) of grapevine (Vitis vinifera) crops: A meta-analysis","authors":"Alexandra Díez-Méndez, Isabel García-Izquierdo, Jorge Poveda","doi":"10.1016/j.cropro.2024.107085","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107085","url":null,"abstract":"The escalating impacts of climate change are exacerbating the spread and intensity of Botrytis bunch rot (BBR), a devastating grapevine disease caused by the necrotrophic fungus <ce:italic>Botrytis cinerea</ce:italic>. Growers have traditionally relied on synthetic fungicides to manage BBR, but the increasing resistance of <ce:italic>B. cinerea</ce:italic> to these chemicals, coupled with growing environmental concerns, has intensified the search for more sustainable and eco-friendly control strategies. Microbial biological control agents (MBCAs) have emerged as a promising alternative for BBR management. This review explores research on using MBCAs to control BBR in grapevine fields. Various microorganisms have shown efficacy, including bacteria such as <ce:italic>Bacillus subtilis</ce:italic>, <ce:italic>B. amyloliquefaciens</ce:italic>, <ce:italic>B. ginsengihumi</ce:italic>, <ce:italic>Pantoea agglomerans</ce:italic> or <ce:italic>Pseudomonas fluorescens</ce:italic>, yeasts such as <ce:italic>Saccharomyces cerevisiae</ce:italic>, <ce:italic>Metschnikowia fructicola</ce:italic> or <ce:italic>Candida sake</ce:italic>, and filamentous fungi such as <ce:italic>Trichoderma asperellum</ce:italic>, <ce:italic>T. pseudokoningii</ce:italic>, <ce:italic>Fusarium solani</ce:italic> or <ce:italic>Ulocladium atrum</ce:italic>. These MBCAs employ diverse mechanisms of action, including direct antagonism through competition for space and nutrients, the production of lytic enzymes that degrade fungal cell walls, and the induction of plant defense mechanisms at both local and systemic levels. However, the effectiveness of these MBCAs can be influenced by environmental factors, with high temperatures and low humidity generally favoring their activity. Further research is needed to optimize MBCA application methods, elucidate the complex interactions between MBCAs, the pathogen, and the grapevine host, and explore the potential of combining multiple MBCAs for enhanced disease control. Integrating MBCAs with other sustainable practices offers a more holistic approach to BBR management, paving the way for a more environmentally friendly and effective strategy to combat this devastating disease.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"258 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13DOI: 10.1016/j.cropro.2024.107083
Yan Wang, Dainan Hao, Haozhe Jiang, Zhaoxiong Fei, Rui Zhao, Jiaxin Gao, Guangxin Li, Chunwei Wang
Codonopsis pilosula, an important genuine herb in Shanxi Province, is generally named Lu Dangshen, and is well-known for its high medicinal value. In 2023, Fusarium wilt, a newly observed severe disease on C. pilosula, is responsible for approximately 40% incidence in the field. It was noticed that the diseased C. pilosula roots were usually asymptomatic on the surface. The typical symptoms were vascular discoloration, wilt, and leaves chlorosis. A total of 87 Fusarium isolates were isolated from diseased samples, and identified as Fusarium oxysporum based on morphological features and partial sequences analyses of internal transcribed spacer region of ribosomal DNA (ITS-rDNA), translation elongation factor 1 alpha (TEF-1α) and endopolygalacturonase 1 (pg1). Of the 87 Fusarium oxysporum isolates, the causal agents were separated into three distinct groups (Groups I, II and III). The virulence of each F. oxysporum isolate to C. pilosula was determined by measuring the disease incidence and Fusarium wilt severity. All the 87 Fusarium oxysporum isolates could cause typical symptoms similar to that originally appeared on diseased plants, and present significantly different pathogenic to C. pilosula. All tested isolates were highly sensitive to jinggangmycin, azoxystrobin, fludioxonil and epoxiconazole (EC50 < 10 μg/mL), and insensitive to iprodione and hymexazol. Additionally, F. oxysporum isolates have a low risk for fungicide resistance to jinggangmycin, iprodione and epoxiconazole. Thus, jinggangmycin and epoxiconazole should be recommended for the control of this disease. Our finding in this study should be useful for the understanding of Fusarium wilt of C. pilosula caused by F. oxysporum.
{"title":"Identification and fungicide sensitivity of Fusarium oxysporum, the cause of Fusarium wilt on Codonopsis pilosula in Shanxi Province (Lu Dangshen), China","authors":"Yan Wang, Dainan Hao, Haozhe Jiang, Zhaoxiong Fei, Rui Zhao, Jiaxin Gao, Guangxin Li, Chunwei Wang","doi":"10.1016/j.cropro.2024.107083","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107083","url":null,"abstract":"<ce:italic>Codonopsis pilosula</ce:italic>, an important genuine herb in Shanxi Province, is generally named Lu Dangshen, and is well-known for its high medicinal value. In 2023, Fusarium wilt, a newly observed severe disease on <ce:italic>C</ce:italic>. <ce:italic>pilosula</ce:italic>, is responsible for approximately 40% incidence in the field. It was noticed that the diseased <ce:italic>C. pilosula</ce:italic> roots were usually asymptomatic on the surface. The typical symptoms were vascular discoloration, wilt, and leaves chlorosis. A total of 87 <ce:italic>Fusarium</ce:italic> isolates were isolated from diseased samples, and identified as <ce:italic>Fusarium oxysporum</ce:italic> based on morphological features and partial sequences analyses of internal transcribed spacer region of ribosomal DNA (ITS-rDNA), translation elongation factor 1 alpha (<ce:italic>TEF-1α</ce:italic>) and endopolygalacturonase 1 (<ce:italic>pg1</ce:italic>). Of the 87 <ce:italic>Fusarium oxysporum</ce:italic> isolates, the causal agents were separated into three distinct groups (Groups I, II and III). The virulence of each <ce:italic>F</ce:italic>. <ce:italic>oxysporum</ce:italic> isolate to <ce:italic>C</ce:italic>. <ce:italic>pilosula</ce:italic> was determined by measuring the disease incidence and Fusarium wilt severity. All the 87 <ce:italic>Fusarium oxysporum</ce:italic> isolates could cause typical symptoms similar to that originally appeared on diseased plants, and present significantly different pathogenic to <ce:italic>C. pilosula</ce:italic>. All tested isolates were highly sensitive to jinggangmycin, azoxystrobin, fludioxonil and epoxiconazole (EC<ce:inf loc=\"post\">50</ce:inf> < 10 μg/mL), and insensitive to iprodione and hymexazol. Additionally, <ce:italic>F. oxysporum</ce:italic> isolates have a low risk for fungicide resistance to jinggangmycin, iprodione and epoxiconazole. Thus, jinggangmycin and epoxiconazole should be recommended for the control of this disease. Our finding in this study should be useful for the understanding of Fusarium wilt of <ce:italic>C</ce:italic>. <ce:italic>pilosula</ce:italic> caused by <ce:italic>F. oxysporum</ce:italic>.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"59 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-13DOI: 10.1016/j.cropro.2024.107084
Mohammad Roozkhosh, Mehdi Rastgoo, Kamal Haj mohammadnia Ghalibaf, Behrooz Khalil Tahmasebi, Ahmad Aien
Purple nutsedge (Cyperus rotundus L.) is a major weed problem worldwide, particularly in onion (Allium cepa L.) fields in Iran. No specific herbicide has been registered or reported for controlling purple nutsedge in onion fields in the country. To address this, two field experiments were conducted over two years (2021 and 2022) at the Jiroft Culture and Industry Company in Jiroft, Kerman, Iran, using a randomized complete block design (RCBD) with 13 treatments and three replications. The experiments aimed to evaluate the effectiveness of various chemical control methods on onion and purple nutsedge growth.The results indicated that all herbicides significantly reduced the stem number, tuber number, and both aboveground and belowground dry weight of purple nutsedge. The highest level of weed control (100%) was achieved with a combination of Florasulam + Fluazifop P butyl + Pretilachlor and Allyl isothiocyanate, with no phytotoxic effects on onions. The highest onion yields were observed in plots treated with Florasulam + Fluazifop P butyl + Pretilachlor (ranging from 75.047 to 71.856 t ha-1) and Allyl isothiocyanate (ranging from 73.618 to 71.323 t ha-1). Furthermore, purple nutsedge competition reduced onion yield by 88.97% and 90.80% compared to the weed-free control over the two years of the experiment.While Council and Krismat herbicides effectively suppressed purple nutsedge growth, they caused damage to the onion plants. Based on the results, the application of Florasulam + Fluazifop P butyl + Pretilachlor (800 g per hectare) and Allyl isothiocyanate (280 L per hectare) is recommended for controlling purple nutsedge in onion fields. Although Allyl isothiocyanate, as a bio-herbicide, showed promising results similar to the chemical herbicide, Florasulam + Fluazifop P butyl + Pretilachlor is more cost-effective, easier to apply, and safer for users.
紫苣苔(Cyperus rotundus L.)是世界范围内的主要杂草问题,特别是在伊朗的洋葱(Allium cepa L.)田。国内尚无专门用于防治洋葱田紫苣苔的除草剂登记或报道。为了解决这个问题,在伊朗Kerman的Jiroft文化和工业公司进行了为期两年(2021年和2022年)的两次现场试验,采用随机完全区组设计(RCBD),有13个处理和3个重复。本试验旨在评价各种化学防治方法对洋葱和紫苣苔生长的效果。结果表明,各除草剂均能显著降低紫苣苔茎数、块茎数和地上、地下干重。使用Florasulam +氟唑磷P丁基+ Pretilachlor和异硫氰酸烯丙酯的组合,达到了最高水平的杂草控制(100%),对洋葱没有植物毒性作用。用Florasulam + Fluazifop P butyl + Pretilachlor (75.047 ~ 71.856 t ha-1)和异硫氰酸烯丙酯(73.618 ~ 71.323 t ha-1)处理的小区洋葱产量最高。在2年的试验中,紫苣苔竞争使洋葱产量分别比无草对照降低了88.97%和90.80%。虽然Council和Krismat除草剂有效地抑制了紫苣苔的生长,但它们对洋葱植物造成了损害。在此基础上,建议应用Florasulam + Fluazifop P butyl + Pretilachlor (800 g /公顷)和异硫氰酸烯丙酯(280 L /公顷)防治洋葱田紫苣苔。虽然异硫氰酸丙烯酯作为一种生物除草剂,表现出与化学除草剂类似的良好效果,但Florasulam + Fluazifop P butyl + Pretilachlor更具成本效益,更容易使用,对用户更安全。
{"title":"Evaluating the efficacy of herbicide options in controlling purple nutsedge (Cyperus rotundus L.) in onion (Allium cepa L.) fields","authors":"Mohammad Roozkhosh, Mehdi Rastgoo, Kamal Haj mohammadnia Ghalibaf, Behrooz Khalil Tahmasebi, Ahmad Aien","doi":"10.1016/j.cropro.2024.107084","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107084","url":null,"abstract":"Purple nutsedge (<ce:italic>Cyperus rotundus</ce:italic> L.) is a major weed problem worldwide, particularly in onion (<ce:italic>Allium cepa</ce:italic> L.) fields in Iran. No specific herbicide has been registered or reported for controlling purple nutsedge in onion fields in the country. To address this, two field experiments were conducted over two years (2021 and 2022) at the Jiroft Culture and Industry Company in Jiroft, Kerman, Iran, using a randomized complete block design (RCBD) with 13 treatments and three replications. The experiments aimed to evaluate the effectiveness of various chemical control methods on onion and purple nutsedge growth.The results indicated that all herbicides significantly reduced the stem number, tuber number, and both aboveground and belowground dry weight of purple nutsedge. The highest level of weed control (100%) was achieved with a combination of Florasulam + Fluazifop P butyl + Pretilachlor and Allyl isothiocyanate, with no phytotoxic effects on onions. The highest onion yields were observed in plots treated with Florasulam + Fluazifop P butyl + Pretilachlor (ranging from 75.047 to 71.856 t ha-1) and Allyl isothiocyanate (ranging from 73.618 to 71.323 t ha-1). Furthermore, purple nutsedge competition reduced onion yield by 88.97% and 90.80% compared to the weed-free control over the two years of the experiment.While Council and Krismat herbicides effectively suppressed purple nutsedge growth, they caused damage to the onion plants. Based on the results, the application of Florasulam + Fluazifop P butyl + Pretilachlor (800 g per hectare) and Allyl isothiocyanate (280 L per hectare) is recommended for controlling purple nutsedge in onion fields. Although Allyl isothiocyanate, as a bio-herbicide, showed promising results similar to the chemical herbicide, Florasulam + Fluazifop P butyl + Pretilachlor is more cost-effective, easier to apply, and safer for users.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"47 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meloidogyne enterolobii can reduce yield and quality of chickpea. Thus far, no resistant cultivars have been reported. Hence, a study was carried out to evaluate the relative performance of biological products for the management of M. enterolobii on chickpea. Greenhouse experiments were conducted with ten treatments (five formulations of antagonistic fungi and bacteria applied either individually or in mixtures) and four controls (non-inoculated and inoculated chickpea and tomato plants). Each plant was inoculated with 4000 eggs and second-stage M. enterolobii juveniles (J2). Evaluation was done 60 days after inoculation for gall index, number of eggs per gram of roots, reproduction factor (RF), plant height, shoot, and root weight. None of the treatments fully suppressed infection. However, a subset of formulation mixtures displayed significant reduction in the levels of damage when compared to the untreated check. The treatments with best performance were [Purpureocillium lilacinum + Trichoderma harzanium] (57–74% reduction) and [Pochonia chlamydosporia + P. lilacinum + Bacillus amyloliquefaciens + B. pumilus + B. subtilis] (58–65% reduction), whereas [P. chlamydosporia + P. lilacinum + T. harzanium] and [B. amyloliquefaciens + B. pumilus + B. subtilis + T. harzanium] displayed the lowest levels of suppression (0–42%). Higher plant height and fresh shoot weight were observed with [P. chlamydosporia + P. lilacinum + B. amyloliquefaciens + B. pumilus + B. subtilis]. Hence, considering the low efficiency of the currently available management methods, the employment of these microbiological products might help to reduce the negative impacts of M. enterolobii in infested fields.
肠孢霉属(Meloidogyne enterolobii)会降低鹰嘴豆的产量和质量。迄今为止,还没有关于抗性栽培品种的报道。因此,我们开展了一项研究,以评估生物产品在管理鹰嘴豆上的肠孢霉菌方面的相对性能。温室试验共进行了 10 个处理(单独或混合使用 5 种拮抗真菌和细菌制剂)和 4 个对照(未接种和接种鹰嘴豆和番茄植株)。每株植物都接种了 4000 个虫卵和第二阶段的肠杆菌幼虫(J2)。接种 60 天后,对虫瘿指数、每克根中的虫卵数、繁殖系数 (RF)、株高、芽和根的重量进行评估。没有一种处理能完全抑制感染。不过,与未处理的对照组相比,部分配方混合物的危害程度明显减轻。表现最好的处理是[紫孢蘑菇 + 哈氏毛霉](减少 57-74%)和[Pochonia chlamydosporia + P. lilacinum + Bacillus amyloliquefaciens + B. pumilus + B. subtilis](减少 58-65%)。而[P. chlamydosporia + P. lilacinum + T. harzanium]和[B. amyloliquefaciens + B. pumilus + B. subtilis + T. harzanium]的抑制程度最低(0-42%)。P. chlamydosporia + P. lilacinum + B. amyloliquefaciens + B. pumilus + B. subtilis]的植株高度和鲜芽重量更高。因此,考虑到目前可用的管理方法效率较低,使用这些微生物产品可能有助于减少肠孢霉对受侵染田块的负面影响。
{"title":"Comparative performance of biological formulations for the management of Meloidogyne enterolobii in chickpea (Cicer arietinum)","authors":"Dwillian Firmiano Cunha, Thávio Júnior Barbosa Pinto, Valdir Ribeiro Correia, Aldegundes Batista Miranda Júnior, Felipe Santos Rafael, Leandro Alves Santos, Érica Vicente dos Santos, Juvenil Enrique Cares, Leonardo Silva Boiteux, Jadir Borges Pinheiro","doi":"10.1016/j.cropro.2024.107082","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107082","url":null,"abstract":"<ce:italic>Meloidogyne enterolobii</ce:italic> can reduce yield and quality of chickpea. Thus far, no resistant cultivars have been reported. Hence, a study was carried out to evaluate the relative performance of biological products for the management of <ce:italic>M. enterolobii</ce:italic> on chickpea. Greenhouse experiments were conducted with ten treatments (five formulations of antagonistic fungi and bacteria applied either individually or in mixtures) and four controls (non-inoculated and inoculated chickpea and tomato plants). Each plant was inoculated with 4000 eggs and second-stage <ce:italic>M. enterolobii</ce:italic> juveniles (J2). Evaluation was done 60 days after inoculation for gall index, number of eggs per gram of roots, reproduction factor (RF), plant height, shoot, and root weight. None of the treatments fully suppressed infection. However, a subset of formulation mixtures displayed significant reduction in the levels of damage when compared to the untreated check. The treatments with best performance were [<ce:italic>Purpureocillium lilacinum</ce:italic> + <ce:italic>Trichoderma harzanium</ce:italic>] (57–74% reduction) and [<ce:italic>Pochonia chlamydosporia</ce:italic> + <ce:italic>P. lilacinum</ce:italic> + <ce:italic>Bacillus amyloliquefaciens</ce:italic> + <ce:italic>B. pumilus</ce:italic> + <ce:italic>B. subtilis</ce:italic>] (58–65% reduction), whereas [<ce:italic>P. chlamydosporia</ce:italic> + <ce:italic>P. lilacinum</ce:italic> + <ce:italic>T. harzanium</ce:italic>] and [<ce:italic>B. amyloliquefaciens</ce:italic> + <ce:italic>B. pumilus</ce:italic> + <ce:italic>B. subtilis</ce:italic> + <ce:italic>T. harzanium</ce:italic>] displayed the lowest levels of suppression (0–42%). Higher plant height and fresh shoot weight were observed with [<ce:italic>P. chlamydosporia</ce:italic> + <ce:italic>P. lilacinum</ce:italic> + <ce:italic>B. amyloliquefaciens</ce:italic> + <ce:italic>B. pumilus</ce:italic> + <ce:italic>B. subtilis</ce:italic>]. Hence, considering the low efficiency of the currently available management methods, the employment of these microbiological products might help to reduce the negative impacts of <ce:italic>M. enterolobii</ce:italic> in infested fields.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"30 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-12DOI: 10.1016/j.cropro.2024.107075
Francisco Garibaldi-Márquez, Daniel A. Martínez-Barba, Luis E. Montañez-Franco, Gerardo Flores, Luis M. Valentín-Coronado
Detection of weeds is essential to implement an intelligent weed control system in natural corn fields. Then, to address this issue, the Swin-UNet, Segmenter, and SegFormer deep learning transformer architectures have been implemented and compared. Furthermore, a simple thresholding method has been performed to enhance the segmentation. Moreover, a large pixel-level annotated image dataset acquired under natural field conditions is introduced to train the models. In addition, the well-known Precision, Dice Similarity Coefficient (DSC), Intersection over Union (IoU), and mean Intersection over Union (mIoU) metrics have been used to evaluate the implemented models’ performance. According to the experimental results, the SegFormer architecture was the best model on each of the three proposed weed detection approaches, achieving a macro performance of up to 94.49%, 95.30%, and 91.26% for Precision, DSC, and mIoU, respectively.
{"title":"Enhancing site-specific weed detection using deep learning transformer architectures","authors":"Francisco Garibaldi-Márquez, Daniel A. Martínez-Barba, Luis E. Montañez-Franco, Gerardo Flores, Luis M. Valentín-Coronado","doi":"10.1016/j.cropro.2024.107075","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107075","url":null,"abstract":"Detection of weeds is essential to implement an intelligent weed control system in natural corn fields. Then, to address this issue, the Swin-UNet, Segmenter, and SegFormer deep learning transformer architectures have been implemented and compared. Furthermore, a simple thresholding method has been performed to enhance the segmentation. Moreover, a large pixel-level annotated image dataset acquired under natural field conditions is introduced to train the models. In addition, the well-known Precision, Dice Similarity Coefficient (DSC), Intersection over Union (IoU), and mean Intersection over Union (mIoU) metrics have been used to evaluate the implemented models’ performance. According to the experimental results, the SegFormer architecture was the best model on each of the three proposed weed detection approaches, achieving a macro performance of up to 94.49%, 95.30%, and 91.26% for Precision, DSC, and mIoU, respectively.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"53 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}