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First report of Botryosphaeria wangensis, Colletotrichum nymphaeae, Diaporthe eres, and Geotrichum candidum causing postharvest fruit rot of plums (Prunus salicina) in China 引起李子采后腐果病的王氏芽孢菌、少女炭疽菌、Diaporthe eres和candidhum在国内首次报道
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-16 DOI: 10.1016/j.cropro.2024.107089
Yu-Ru Wang, Bo-Ying Huang, Hafiz Muhammad Usman, Khadija Javed, Fatimah Al-Otibi, Kevin D. Hyde, Yong Wang
Plums (Prunus salicina) are highly valued for their diverse flavors and nutritional benefits, making them essential ingredients in processed foods such as juices and jams, making them one of the world's most popular fruits. However, their postharvest short shelf life, compounded by rapid deterioration and rot, presents significant challenges to maintaining fruit quality and extending shelf stability, resulting in substantial economic losses for the industry. This study employed both morphological and molecular biology approaches to identify and characterize pathogens from diseased plum samples collected in major production regions. Key pathogens responsible for postharvest fruit rot in plums in Guizhou, China, were identified as Botryosphaeria wangensis, Colletotrichum nymphaeae, Diaporthe eres, and Geotrichum candidum. The comprehensive identification of these causative agents is crucial for developing targeted disease management strategies. This research contributes crucial insights for enhancing plum quality and extending shelf stability in the industry, addressing essential needs in fruit preservation and economic sustainability.
李子(Prunus salicina)因其多样的风味和营养价值而受到高度重视,使其成为果汁和果酱等加工食品的重要成分,使其成为世界上最受欢迎的水果之一。然而,它们采后的保质期短,加上快速变质和腐烂,对保持水果质量和延长货架稳定性提出了重大挑战,给该行业造成了巨大的经济损失。本研究采用形态学和分子生物学相结合的方法,对主要产区李子病样进行病原鉴定和鉴定。鉴定了造成贵州李子采后果实腐烂的主要病原菌为王氏芽孢菌(Botryosphaeria wangensis)、淋巴炭疽菌(Colletotrichum nymphaeae)、炭疽菌(Diaporthe eres)和白土霉(Geotrichum candidum)。全面识别这些病原体对于制定有针对性的疾病管理策略至关重要。该研究为提高李子品质和延长工业货架稳定性,解决水果保鲜和经济可持续性的基本需求提供了重要见解。
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引用次数: 0
Sensitivity monitoring of Bemisia tabaci to two tetramic acid insecticides and baseline susceptibility to spiropidion in China 中国烟粉虱对两种四羧酸类杀虫剂的敏感性监测及对螺必啶的基线敏感性
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-16 DOI: 10.1016/j.cropro.2024.107088
Xiaolan Liu, Xichao Hu, Zanrong Wen, Haoyu Tang, Jianyu Ma, Bo Qiu, Wei Xu, Xiuli Chi, Chao Lv, Lei Guo, Yongjun Zhang
The sweet potato whitefly, Bemisia tabaci (Gennadius) is one of the most devastating pests, inflicting severe damage on a wide range of crops. The tetramic acid insecticides, spirotetramat and spiropidion, act as inhibitors of lipid biosynthesis by targeting acetyl-coenzyme A carboxylase (ACCase), disrupting fatty acid biosynthesis and energy metabolism. In the present study, a total of 47 field populations were collected across China in 2021 and 2022, and their susceptibilities to spirotetramat and spiropidion were determined in the laboratory. The results showed that in contrast to their toxicities against nymphs, spirotetramat and spiropidion exhibited minimal toxicity against B. tabaci adults. B. tabaci nymphs from field populations exhibited susceptibility or low resistance to spirotetramat, with LC50 values ranging from 2.85 to 13.58 mg L−1 and resistance ratio (RR) from 1.7 to 8.2. There was a variation in the sensitivity of B. tabaci field populations towards spiropidion, with LC50 values ranging from 13.12 to 120.13 mg L−1 and RR from 3.5 to 23.4. The baseline susceptibility of B. tabaci nymphs to spiropidion was determined to be 24.06 mg L⁻1, corresponding to the median lethal concentration (LC50) calculated from 43 field populations. Cross-resistance was observed between spirotetramat and spiropidion, as well as between cyantraniliprole and tetramic acid insecticides. However, no significant cross-resistance was found between neonicotinoids and tetramic acid insecticides. Collectively, these findings improve our knowledge on the toxicity of tetramic acid insecticides to B. tabaci populations in China and provide valuable information for their scientific application in the field.
甘薯粉虱,烟粉虱(Gennadius)是最具破坏性的害虫之一,对多种作物造成严重损害。四羧酸类杀虫剂螺虫曲麦和螺虫啶通过靶向乙酰辅酶A羧化酶(ACCase),破坏脂肪酸的生物合成和能量代谢,作为脂类生物合成的抑制剂。本研究于2021年和2022年在中国共采集了47个野外种群,并在实验室测定了它们对螺旋体和螺旋体的敏感性。结果表明,与对若虫的毒力相比,螺虫虫和螺虫啶对烟粉虱成虫的毒力最小。田间种群烟粉虱若虫对螺虫呈敏感性或低抗性,LC50值为2.85 ~ 13.58 mg L−1,抗性比(RR)为1.7 ~ 8.2。烟粉虱田间种群对吡虫啶的敏感性存在差异,LC50值为13.12 ~ 120.13 mg L−1,RR值为3.5 ~ 23.4。烟粉虱若虫对螺皮丁的基线敏感性为24.06 mg L - 1,与43个野外种群计算的中位致死浓度(LC50)相对应。螺虫与螺虫腈、氰虫腈与四酸杀虫剂之间存在交叉抗性。新烟碱类杀虫剂与四羧酸类杀虫剂间无明显交叉抗性。总的来说,这些发现提高了我们对四羧酸杀虫剂对中国烟粉虱种群毒性的认识,并为其在野外的科学应用提供了有价值的信息。
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引用次数: 0
Effects of difenoconazole and pyraclostrobin on the development, reproduction, and detoxifying metabolism of Spodoptera frugiperda (J.E. Smith) 异丙唑康唑和嘧菌酯对果夜蛾发育、繁殖和解毒代谢的影响
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-15 DOI: 10.1016/j.cropro.2024.107081
Shuqi Yang, Yue Yuan, Xiongwei Zhang, Aoju Feng, Yaqin Zou, Pan Yao, Danni Ye, Kaiyi Zeng, Xiaolei Zhang, Zhihong Xu
<ce:italic>Spodoptera frugiperda</ce:italic> (J.E. Smith) is a major insect pest affecting corn globally. <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> often coincides with maize diseases, as their damage periods overlap. Consequently, <ce:italic>S. frugiperda</ce:italic> may be exposed to varying types and quantities of these fungicides during the growing season. The aim of this study was to determine the effects of difenoconazole and pyraclostrobin on the biological and physiological traits of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Treatment with difenoconazole and pyraclostrobin significantly prolonged the pupal development period in the F<ce:inf loc="post">0</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic> compared to the control group. Additionally, exposure to difenoconazole shortened the development periods of the 1st and 6th instar larvae and reduced the adult preoviposition period (APOP) in the F<ce:inf loc="post">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>. However, it extended the development period of the 4th instar larvae and pupae, as well as overall longevity. The pyraclostrobin treatment group exhibited a shorter development period of 2nd instar larvae, as well as longer longevity, and more eggs/per female in the F<ce:inf loc="post">1</ce:inf> generation of <ce:italic>S</ce:italic>. <ce:italic>frugiperda</ce:italic>. Compared to the control group, the fecundity of the offspring of the pyraclostrobin treatment group increased by 68.24%. Furthermore, treatment with difenoconazole and pyraclostrobin significantly increased the intrinsic rate of increase (<ce:italic>r</ce:italic>) and the finite rate of increase (<ce:italic>λ</ce:italic>) in the F<ce:inf loc="post">1</ce:inf> generation of <ce:italic>S. frugiperda</ce:italic>, while significantly reducing the mean generation time (<ce:italic>T</ce:italic>). Additionally, mRNA expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP321A10</ce:italic>, <ce:italic>CYP341A11</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13059</ce:italic>, <ce:italic>CXE23</ce:italic>, <ce:italic>ABC01998, ABC18240</ce:italic>, and <ce:italic>UGT17742</ce:italic> were significantly elevated in F<ce:inf loc="post">0</ce:inf> individuals from larvae treated with difenoconazole. Meanwhile, the relative expression levels of <ce:italic>CYP6AN4</ce:italic>, <ce:italic>CYP340L1</ce:italic>, <ce:italic>CYP314A1</ce:italic>, <ce:italic>GST19</ce:italic>, <ce:italic>GST22</ce:italic>, <ce:italic>CE13095</ce:italic>, <ce:italic>CXE20</ce:italic>, <ce:italic>ABC01998</ce:italic>, <ce:italic>ABC18240</ce:italic>, <ce:italic>ABC22158</ce:italic>, and <ce:italic>ABC22158</ce:italic> in the larvae (F<ce:inf loc="post">0</ce:inf>) exposed to pyraclostrobin were also elevated. Compared to the control and difenoconazole treatment groups, the expression of
Spodoptera frugiperda (J.E. Smith)是影响全球玉米的主要害虫。frugiperda通常与玉米病害同时发生,因为它们的危害期重叠。因此,在生长季节,frugiperda可能暴露于不同类型和数量的这些杀菌剂。本研究的目的是研究异丙康唑和吡氯菌酯对果螨的生物学和生理特性的影响。与对照组相比,异丙康唑和吡氯菌酯处理显著延长了F0代frugiperda的蛹期。此外,异苯醚康唑还能显著缩短果螨F1代1龄和6龄幼虫的发育周期,降低成虫产卵前期(APOP)。然而,它延长了4龄幼虫和蛹的发育周期,并延长了整体寿命。吡唑菌酯处理组2龄幼虫发育周期较短,寿命较长,F1代雌虫产卵数较多。与对照组相比,吡唑菌酯处理组的子代繁殖力提高了68.24%。此外,二苯醚康唑和吡氯虫酯处理显著提高了frugiperda F1代的内在增长率(r)和有限增长率(λ),显著缩短了平均一代时间(T)。此外,二苯醚康唑处理F0个个体的CYP6AN4、CYP340L1、CYP321A10、CYP341A11、GST19、GST22、CE13059、CXE23、ABC01998、ABC18240和UGT17742 mRNA表达量显著升高。同时,CYP6AN4、CYP340L1、CYP314A1、GST19、GST22、CE13095、CXE20、ABC01998、ABC18240、ABC22158、ABC22158在pyraclostrobin作用下的幼虫(F0)中的相对表达量也有所升高。pyraclostrobin处理后,与对照组和异苯唑唑处理组相比,卵黄蛋白原基因(Vg)和卵黄蛋白原受体(VgR)表达显著上调。研究了异虫康唑和嘧菌酯对果螨的生物学和繁殖性能的影响,为优化果螨的管理策略提供了依据。
{"title":"Effects of difenoconazole and pyraclostrobin on the development, reproduction, and detoxifying metabolism of Spodoptera frugiperda (J.E. Smith)","authors":"Shuqi Yang, Yue Yuan, Xiongwei Zhang, Aoju Feng, Yaqin Zou, Pan Yao, Danni Ye, Kaiyi Zeng, Xiaolei Zhang, Zhihong Xu","doi":"10.1016/j.cropro.2024.107081","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107081","url":null,"abstract":"&lt;ce:italic&gt;Spodoptera frugiperda&lt;/ce:italic&gt; (J.E. Smith) is a major insect pest affecting corn globally. &lt;ce:italic&gt;S&lt;/ce:italic&gt;. &lt;ce:italic&gt;frugiperda&lt;/ce:italic&gt; often coincides with maize diseases, as their damage periods overlap. Consequently, &lt;ce:italic&gt;S. frugiperda&lt;/ce:italic&gt; may be exposed to varying types and quantities of these fungicides during the growing season. The aim of this study was to determine the effects of difenoconazole and pyraclostrobin on the biological and physiological traits of &lt;ce:italic&gt;S&lt;/ce:italic&gt;. &lt;ce:italic&gt;frugiperda&lt;/ce:italic&gt;. Treatment with difenoconazole and pyraclostrobin significantly prolonged the pupal development period in the F&lt;ce:inf loc=\"post\"&gt;0&lt;/ce:inf&gt; generation of &lt;ce:italic&gt;S&lt;/ce:italic&gt;. &lt;ce:italic&gt;frugiperda&lt;/ce:italic&gt; compared to the control group. Additionally, exposure to difenoconazole shortened the development periods of the 1st and 6th instar larvae and reduced the adult preoviposition period (APOP) in the F&lt;ce:inf loc=\"post\"&gt;1&lt;/ce:inf&gt; generation of &lt;ce:italic&gt;S. frugiperda&lt;/ce:italic&gt;. However, it extended the development period of the 4th instar larvae and pupae, as well as overall longevity. The pyraclostrobin treatment group exhibited a shorter development period of 2nd instar larvae, as well as longer longevity, and more eggs/per female in the F&lt;ce:inf loc=\"post\"&gt;1&lt;/ce:inf&gt; generation of &lt;ce:italic&gt;S&lt;/ce:italic&gt;. &lt;ce:italic&gt;frugiperda&lt;/ce:italic&gt;. Compared to the control group, the fecundity of the offspring of the pyraclostrobin treatment group increased by 68.24%. Furthermore, treatment with difenoconazole and pyraclostrobin significantly increased the intrinsic rate of increase (&lt;ce:italic&gt;r&lt;/ce:italic&gt;) and the finite rate of increase (&lt;ce:italic&gt;λ&lt;/ce:italic&gt;) in the F&lt;ce:inf loc=\"post\"&gt;1&lt;/ce:inf&gt; generation of &lt;ce:italic&gt;S. frugiperda&lt;/ce:italic&gt;, while significantly reducing the mean generation time (&lt;ce:italic&gt;T&lt;/ce:italic&gt;). Additionally, mRNA expression levels of &lt;ce:italic&gt;CYP6AN4&lt;/ce:italic&gt;, &lt;ce:italic&gt;CYP340L1&lt;/ce:italic&gt;, &lt;ce:italic&gt;CYP321A10&lt;/ce:italic&gt;, &lt;ce:italic&gt;CYP341A11&lt;/ce:italic&gt;, &lt;ce:italic&gt;GST19&lt;/ce:italic&gt;, &lt;ce:italic&gt;GST22&lt;/ce:italic&gt;, &lt;ce:italic&gt;CE13059&lt;/ce:italic&gt;, &lt;ce:italic&gt;CXE23&lt;/ce:italic&gt;, &lt;ce:italic&gt;ABC01998, ABC18240&lt;/ce:italic&gt;, and &lt;ce:italic&gt;UGT17742&lt;/ce:italic&gt; were significantly elevated in F&lt;ce:inf loc=\"post\"&gt;0&lt;/ce:inf&gt; individuals from larvae treated with difenoconazole. Meanwhile, the relative expression levels of &lt;ce:italic&gt;CYP6AN4&lt;/ce:italic&gt;, &lt;ce:italic&gt;CYP340L1&lt;/ce:italic&gt;, &lt;ce:italic&gt;CYP314A1&lt;/ce:italic&gt;, &lt;ce:italic&gt;GST19&lt;/ce:italic&gt;, &lt;ce:italic&gt;GST22&lt;/ce:italic&gt;, &lt;ce:italic&gt;CE13095&lt;/ce:italic&gt;, &lt;ce:italic&gt;CXE20&lt;/ce:italic&gt;, &lt;ce:italic&gt;ABC01998&lt;/ce:italic&gt;, &lt;ce:italic&gt;ABC18240&lt;/ce:italic&gt;, &lt;ce:italic&gt;ABC22158&lt;/ce:italic&gt;, and &lt;ce:italic&gt;ABC22158&lt;/ce:italic&gt; in the larvae (F&lt;ce:inf loc=\"post\"&gt;0&lt;/ce:inf&gt;) exposed to pyraclostrobin were also elevated. Compared to the control and difenoconazole treatment groups, the expression of ","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"90 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142841460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Ephestia elutella diets on fitness of the third trophic level, the parasitoid Habrobracon hebetor (Hymenoptera: Braconidae) 乳黄小蜂饵料对第三营养级拟寄主小蜂适合度的影响(膜翅目:小蜂科)
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-15 DOI: 10.1016/j.cropro.2024.107079
Yao Huang, Xiuqin Wang, Houding Ou, Lan Wei, Xiaofei Yu, Bo Wang, Yi Mao, Maofa Yang
In the three-level nutritional structure of plant–phytophagous insect–natural enemies, plants exert direct and indirect effects on natural enemies. Additionally, the effect of the host diet on natural enemies also has an indirect impact on pest control. Herein, the effects of two diets of Ephestia elutella on the fitness of its natural enemy Habrobracon hebetor were studied using an age–stage, two-sex life table analysis. The results indicated that the total fecundity, the number of eggs laid during each oviposition day (Ed), oviposition days, and female longevity of the H. hebetor of F0 generation parasitized on E. elutella fed an artificial diet were significantly higher than those on E. elutella fed tobacco leaves. The pre-adult survival, paralysis, and parasitism rates of the H. hebetor F1 generation parasitized on E. elutella fed an artificial diet were also significantly higher than those on E. elutella fed tobacco leaves. The results provide a theoretical basis for the large-scale propagation of H. hebetor in the laboratory and for improving the efficiency of H. hebetor in controlling E. elutella in tobacco warehouses.
在植物-植食昆虫-天敌的三级营养结构中,植物对天敌有直接和间接的作用。此外,寄主对天敌的食性影响也对害虫防治产生间接影响。本文采用年龄分期、两性寿命表分析法,研究了两种饲料对艾弗estia elutella天敌habrobron hebetor适合度的影响。结果表明:饲喂人工饲料的乳臭虫第0代的总产卵量、产卵日数、产卵日数和雌虫寿命显著高于饲喂烟叶的乳臭虫。寄生在人工饲粮上的褐家鼠F1代成虫前成虫存活率、麻痹率和寄生率均显著高于采食烟叶的褐家鼠。该研究结果为在实验室中大规模繁殖和提高烟仓内黑僵菌的防治效率提供了理论依据。
{"title":"Effect of Ephestia elutella diets on fitness of the third trophic level, the parasitoid Habrobracon hebetor (Hymenoptera: Braconidae)","authors":"Yao Huang, Xiuqin Wang, Houding Ou, Lan Wei, Xiaofei Yu, Bo Wang, Yi Mao, Maofa Yang","doi":"10.1016/j.cropro.2024.107079","DOIUrl":"https://doi.org/10.1016/j.cropro.2024.107079","url":null,"abstract":"In the three-level nutritional structure of plant–phytophagous insect–natural enemies, plants exert direct and indirect effects on natural enemies. Additionally, the effect of the host diet on natural enemies also has an indirect impact on pest control. Herein, the effects of two diets of <ce:italic>Ephestia elutella</ce:italic> on the fitness of its natural enemy <ce:italic>Habrobracon hebetor</ce:italic> were studied using an age–stage, two-sex life table analysis. The results indicated that the total fecundity, the number of eggs laid during each oviposition day (<ce:italic>Ed</ce:italic>), oviposition days, and female longevity of the <ce:italic>H. hebetor</ce:italic> of F<ce:inf loc=\"post\">0</ce:inf> generation parasitized on <ce:italic>E. elutella</ce:italic> fed an artificial diet were significantly higher than those on <ce:italic>E. elutella</ce:italic> fed tobacco leaves. The pre-adult survival, paralysis, and parasitism rates of the <ce:italic>H. hebetor</ce:italic> F<ce:inf loc=\"post\">1</ce:inf> generation parasitized on <ce:italic>E. elutella</ce:italic> fed an artificial diet were also significantly higher than those on <ce:italic>E. elutella</ce:italic> fed tobacco leaves. The results provide a theoretical basis for the large-scale propagation of <ce:italic>H. hebetor</ce:italic> in the laboratory and for improving the efficiency of <ce:italic>H. hebetor</ce:italic> in controlling <ce:italic>E. elutella</ce:italic> in tobacco warehouses.","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"41 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142884073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Management of Phytophthora and Phytopythium oomycete diseases in durian (Duriozibethinus) 榴莲疫霉病和卵菌病的防治
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-15 DOI: 10.1016/j.cropro.2024.107086
Ajit Singh, Caryn Chow, Kevin Nathaniel, Yap Lip Vun, Sumera Javad, Khajista Jabeen
Durian (Durio zibethinus L.) is a valuable fruit crop originating from Borneo and widely cultivated in Southeast Asia, including Malaysia, Thailand, and Indonesia. Increasing consumer demand, particularly from China, has boosted its trade and value. However, the durian industry faces significant challenges from the devastating pathogens Phytophthora palmivora and Phytopythium vexans, which cause various forms of rot and dieback, leading to economic losses. Current management strategies include cultural practices, synthetic fungicides, biological controls, and integrated management. While cultural practices and synthetic fungicides are commonly used, they have limitations such as health and environmental risks, labor intensity, and inconsistent effectiveness. Alternative approaches, such as gene silencing of Protein Phosphatase 2A to produce disease-resistant plants and the use of plant extracts with antimicrobial properties, show promise and warrant further exploration. This review aims to guide farmers and beginners in exploring alternative methods for managing durian diseases.
榴莲(Durio zibethinus L.)是一种珍贵的水果作物,原产于婆罗洲,在马来西亚、泰国和印度尼西亚等东南亚国家广泛种植。日益增长的消费需求,尤其是来自中国的需求,促进了榴莲的贸易和价值。然而,榴莲产业面临着毁灭性病原体棕榈疫霉菌(Phytophthora palmivora)和矢车菊疫霉菌(Phytopythium vexans)的巨大挑战,这两种病原体会造成各种形式的腐烂和枯萎,从而导致经济损失。目前的管理策略包括栽培措施、合成杀菌剂、生物防治和综合管理。文化措施和合成杀菌剂是常用的方法,但它们也有局限性,如健康和环境风险、劳动强度和效果不稳定。其他方法,如通过基因沉默蛋白磷酸酶 2A 来培育抗病植物,以及使用具有抗菌特性的植物提取物,都显示出良好的前景,值得进一步探索。本综述旨在指导农民和初学者探索管理榴莲病害的替代方法。
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引用次数: 0
Microbial biological control of Botrytis bunch rot (Botrytis cinerea) of grapevine (Vitis vinifera) crops: A meta-analysis 葡萄(Vitis vinifera)作物串腐病(Botrytis cinerea)的微生物控制:荟萃分析
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-15 DOI: 10.1016/j.cropro.2024.107085
Alexandra Díez-Méndez, Isabel García-Izquierdo, Jorge Poveda
The escalating impacts of climate change are exacerbating the spread and intensity of Botrytis bunch rot (BBR), a devastating grapevine disease caused by the necrotrophic fungus Botrytis cinerea. Growers have traditionally relied on synthetic fungicides to manage BBR, but the increasing resistance of B. cinerea to these chemicals, coupled with growing environmental concerns, has intensified the search for more sustainable and eco-friendly control strategies. Microbial biological control agents (MBCAs) have emerged as a promising alternative for BBR management. This review explores research on using MBCAs to control BBR in grapevine fields. Various microorganisms have shown efficacy, including bacteria such as Bacillus subtilis, B. amyloliquefaciens, B. ginsengihumi, Pantoea agglomerans or Pseudomonas fluorescens, yeasts such as Saccharomyces cerevisiae, Metschnikowia fructicola or Candida sake, and filamentous fungi such as Trichoderma asperellum, T. pseudokoningii, Fusarium solani or Ulocladium atrum. These MBCAs employ diverse mechanisms of action, including direct antagonism through competition for space and nutrients, the production of lytic enzymes that degrade fungal cell walls, and the induction of plant defense mechanisms at both local and systemic levels. However, the effectiveness of these MBCAs can be influenced by environmental factors, with high temperatures and low humidity generally favoring their activity. Further research is needed to optimize MBCA application methods, elucidate the complex interactions between MBCAs, the pathogen, and the grapevine host, and explore the potential of combining multiple MBCAs for enhanced disease control. Integrating MBCAs with other sustainable practices offers a more holistic approach to BBR management, paving the way for a more environmentally friendly and effective strategy to combat this devastating disease.
由坏死性真菌 Botrytis cinerea 引起的破坏性葡萄病害--丛腐病(BBR)的蔓延和严重程度因气候变化的影响而加剧。种植者传统上一直依赖合成杀菌剂来控制丛腐病,但由于灰葡萄孢菌对这些化学品的抗药性越来越强,再加上对环境的日益关注,人们开始寻求更可持续、更环保的控制策略。微生物生物控制剂(MBCA)已成为一种很有前景的 BBR 管理替代方法。本综述探讨了在葡萄田中使用微生物生物控制剂控制 BBR 的研究。各种微生物已显示出功效,包括枯草芽孢杆菌、淀粉芽孢杆菌、人参酵母菌、泛氏聚合菌或荧光假单胞菌等细菌,酿酒酵母菌、果味酵母菌或清酒念珠菌等酵母菌,以及毛霉菌、假孔菌、茄科镰刀菌或Ulocladium atrum等丝状真菌。这些多囊菌素的作用机制多种多样,包括通过竞争空间和养分直接产生拮抗作用、产生可降解真菌细胞壁的溶解酶,以及在局部和系统水平诱导植物防御机制。不过,这些多溴联苯醚的效果会受到环境因素的影响,高温和低湿度通常有利于它们的活性。还需要进一步研究,以优化多溴联苯醚的施用方法,阐明多溴联苯醚、病原体和葡萄寄主之间复杂的相互作用,并探索将多种多溴联苯醚结合起来加强病害控制的潜力。将多溴联苯醚与其他可持续发展实践相结合,可为BBR管理提供更全面的方法,为采用更环保、更有效的策略防治这种毁灭性病害铺平道路。
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引用次数: 0
Identification and fungicide sensitivity of Fusarium oxysporum, the cause of Fusarium wilt on Codonopsis pilosula in Shanxi Province (Lu Dangshen), China 山西党参枯萎病病原菌尖孢镰刀菌的鉴定及其对杀菌剂的敏感性
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-13 DOI: 10.1016/j.cropro.2024.107083
Yan Wang, Dainan Hao, Haozhe Jiang, Zhaoxiong Fei, Rui Zhao, Jiaxin Gao, Guangxin Li, Chunwei Wang
Codonopsis pilosula, an important genuine herb in Shanxi Province, is generally named Lu Dangshen, and is well-known for its high medicinal value. In 2023, Fusarium wilt, a newly observed severe disease on C. pilosula, is responsible for approximately 40% incidence in the field. It was noticed that the diseased C. pilosula roots were usually asymptomatic on the surface. The typical symptoms were vascular discoloration, wilt, and leaves chlorosis. A total of 87 Fusarium isolates were isolated from diseased samples, and identified as Fusarium oxysporum based on morphological features and partial sequences analyses of internal transcribed spacer region of ribosomal DNA (ITS-rDNA), translation elongation factor 1 alpha (TEF-1α) and endopolygalacturonase 1 (pg1). Of the 87 Fusarium oxysporum isolates, the causal agents were separated into three distinct groups (Groups I, II and III). The virulence of each F. oxysporum isolate to C. pilosula was determined by measuring the disease incidence and Fusarium wilt severity. All the 87 Fusarium oxysporum isolates could cause typical symptoms similar to that originally appeared on diseased plants, and present significantly different pathogenic to C. pilosula. All tested isolates were highly sensitive to jinggangmycin, azoxystrobin, fludioxonil and epoxiconazole (EC50 < 10 μg/mL), and insensitive to iprodione and hymexazol. Additionally, F. oxysporum isolates have a low risk for fungicide resistance to jinggangmycin, iprodione and epoxiconazole. Thus, jinggangmycin and epoxiconazole should be recommended for the control of this disease. Our finding in this study should be useful for the understanding of Fusarium wilt of C. pilosula caused by F. oxysporum.
党参是山西省重要的正宗草药,俗称陆党参,因其极高的药用价值而闻名。在2023年,新发现的一种严重病害镰刀菌枯萎病(Fusarium wilt)在田间的发病率约为40%。我们注意到患病的党参根部在表面上通常是无症状的。典型症状为血管变色、枯萎和叶片褪绿。从病株中分离到87株镰刀菌,通过形态特征和核糖体DNA内部转录间隔区(ITS-rDNA)、翻译延伸因子1α (TEF-1α)和内聚半乳糖酶1 (pg1)的部分序列分析,鉴定为尖孢镰刀菌。将87株尖孢镰刀菌分离物分为3组(I、II和III组),通过测定病原菌发病率和枯萎病严重程度来测定各分离物对党参的毒力。87株尖孢镰刀菌分离株均能引起与原病株相似的典型症状,对草梗镰刀菌的致病性存在显著差异。所有分离株均对井冈霉素、嘧菌酯、氟恶菌腈和环氧康唑高度敏感(EC50 <;10 μg/mL),对异丙酮和噻虫唑不敏感。此外,尖孢F. oxysporum菌株对井冈霉素、异丙二酮和环氧康唑的耐药风险较低。因此,应推荐使用井冈霉素和环氧康唑控制本病。本研究结果将有助于对尖孢镰刀菌引起的党参枯萎病的认识。
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引用次数: 0
Evaluating the efficacy of herbicide options in controlling purple nutsedge (Cyperus rotundus L.) in onion (Allium cepa L.) fields 评价几种除草剂对洋葱紫苣苔(Cyperus rotundus L.)的防治效果
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-13 DOI: 10.1016/j.cropro.2024.107084
Mohammad Roozkhosh, Mehdi Rastgoo, Kamal Haj mohammadnia Ghalibaf, Behrooz Khalil Tahmasebi, Ahmad Aien
Purple nutsedge (Cyperus rotundus L.) is a major weed problem worldwide, particularly in onion (Allium cepa L.) fields in Iran. No specific herbicide has been registered or reported for controlling purple nutsedge in onion fields in the country. To address this, two field experiments were conducted over two years (2021 and 2022) at the Jiroft Culture and Industry Company in Jiroft, Kerman, Iran, using a randomized complete block design (RCBD) with 13 treatments and three replications. The experiments aimed to evaluate the effectiveness of various chemical control methods on onion and purple nutsedge growth.The results indicated that all herbicides significantly reduced the stem number, tuber number, and both aboveground and belowground dry weight of purple nutsedge. The highest level of weed control (100%) was achieved with a combination of Florasulam + Fluazifop P butyl + Pretilachlor and Allyl isothiocyanate, with no phytotoxic effects on onions. The highest onion yields were observed in plots treated with Florasulam + Fluazifop P butyl + Pretilachlor (ranging from 75.047 to 71.856 t ha-1) and Allyl isothiocyanate (ranging from 73.618 to 71.323 t ha-1). Furthermore, purple nutsedge competition reduced onion yield by 88.97% and 90.80% compared to the weed-free control over the two years of the experiment.While Council and Krismat herbicides effectively suppressed purple nutsedge growth, they caused damage to the onion plants. Based on the results, the application of Florasulam + Fluazifop P butyl + Pretilachlor (800 g per hectare) and Allyl isothiocyanate (280 L per hectare) is recommended for controlling purple nutsedge in onion fields. Although Allyl isothiocyanate, as a bio-herbicide, showed promising results similar to the chemical herbicide, Florasulam + Fluazifop P butyl + Pretilachlor is more cost-effective, easier to apply, and safer for users.
紫苣苔(Cyperus rotundus L.)是世界范围内的主要杂草问题,特别是在伊朗的洋葱(Allium cepa L.)田。国内尚无专门用于防治洋葱田紫苣苔的除草剂登记或报道。为了解决这个问题,在伊朗Kerman的Jiroft文化和工业公司进行了为期两年(2021年和2022年)的两次现场试验,采用随机完全区组设计(RCBD),有13个处理和3个重复。本试验旨在评价各种化学防治方法对洋葱和紫苣苔生长的效果。结果表明,各除草剂均能显著降低紫苣苔茎数、块茎数和地上、地下干重。使用Florasulam +氟唑磷P丁基+ Pretilachlor和异硫氰酸烯丙酯的组合,达到了最高水平的杂草控制(100%),对洋葱没有植物毒性作用。用Florasulam + Fluazifop P butyl + Pretilachlor (75.047 ~ 71.856 t ha-1)和异硫氰酸烯丙酯(73.618 ~ 71.323 t ha-1)处理的小区洋葱产量最高。在2年的试验中,紫苣苔竞争使洋葱产量分别比无草对照降低了88.97%和90.80%。虽然Council和Krismat除草剂有效地抑制了紫苣苔的生长,但它们对洋葱植物造成了损害。在此基础上,建议应用Florasulam + Fluazifop P butyl + Pretilachlor (800 g /公顷)和异硫氰酸烯丙酯(280 L /公顷)防治洋葱田紫苣苔。虽然异硫氰酸丙烯酯作为一种生物除草剂,表现出与化学除草剂类似的良好效果,但Florasulam + Fluazifop P butyl + Pretilachlor更具成本效益,更容易使用,对用户更安全。
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引用次数: 0
Comparative performance of biological formulations for the management of Meloidogyne enterolobii in chickpea (Cicer arietinum) 鹰嘴豆肠曲线虫生物制剂的防治效果比较
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-13 DOI: 10.1016/j.cropro.2024.107082
Dwillian Firmiano Cunha, Thávio Júnior Barbosa Pinto, Valdir Ribeiro Correia, Aldegundes Batista Miranda Júnior, Felipe Santos Rafael, Leandro Alves Santos, Érica Vicente dos Santos, Juvenil Enrique Cares, Leonardo Silva Boiteux, Jadir Borges Pinheiro
Meloidogyne enterolobii can reduce yield and quality of chickpea. Thus far, no resistant cultivars have been reported. Hence, a study was carried out to evaluate the relative performance of biological products for the management of M. enterolobii on chickpea. Greenhouse experiments were conducted with ten treatments (five formulations of antagonistic fungi and bacteria applied either individually or in mixtures) and four controls (non-inoculated and inoculated chickpea and tomato plants). Each plant was inoculated with 4000 eggs and second-stage M. enterolobii juveniles (J2). Evaluation was done 60 days after inoculation for gall index, number of eggs per gram of roots, reproduction factor (RF), plant height, shoot, and root weight. None of the treatments fully suppressed infection. However, a subset of formulation mixtures displayed significant reduction in the levels of damage when compared to the untreated check. The treatments with best performance were [Purpureocillium lilacinum + Trichoderma harzanium] (57–74% reduction) and [Pochonia chlamydosporia + P. lilacinum + Bacillus amyloliquefaciens + B. pumilus + B. subtilis] (58–65% reduction), whereas [P. chlamydosporia + P. lilacinum + T. harzanium] and [B. amyloliquefaciens + B. pumilus + B. subtilis + T. harzanium] displayed the lowest levels of suppression (0–42%). Higher plant height and fresh shoot weight were observed with [P. chlamydosporia + P. lilacinum + B. amyloliquefaciens + B. pumilus + B. subtilis]. Hence, considering the low efficiency of the currently available management methods, the employment of these microbiological products might help to reduce the negative impacts of M. enterolobii in infested fields.
肠孢霉属(Meloidogyne enterolobii)会降低鹰嘴豆的产量和质量。迄今为止,还没有关于抗性栽培品种的报道。因此,我们开展了一项研究,以评估生物产品在管理鹰嘴豆上的肠孢霉菌方面的相对性能。温室试验共进行了 10 个处理(单独或混合使用 5 种拮抗真菌和细菌制剂)和 4 个对照(未接种和接种鹰嘴豆和番茄植株)。每株植物都接种了 4000 个虫卵和第二阶段的肠杆菌幼虫(J2)。接种 60 天后,对虫瘿指数、每克根中的虫卵数、繁殖系数 (RF)、株高、芽和根的重量进行评估。没有一种处理能完全抑制感染。不过,与未处理的对照组相比,部分配方混合物的危害程度明显减轻。表现最好的处理是[紫孢蘑菇 + 哈氏毛霉](减少 57-74%)和[Pochonia chlamydosporia + P. lilacinum + Bacillus amyloliquefaciens + B. pumilus + B. subtilis](减少 58-65%)。而[P. chlamydosporia + P. lilacinum + T. harzanium]和[B. amyloliquefaciens + B. pumilus + B. subtilis + T. harzanium]的抑制程度最低(0-42%)。P. chlamydosporia + P. lilacinum + B. amyloliquefaciens + B. pumilus + B. subtilis]的植株高度和鲜芽重量更高。因此,考虑到目前可用的管理方法效率较低,使用这些微生物产品可能有助于减少肠孢霉对受侵染田块的负面影响。
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引用次数: 0
Enhancing site-specific weed detection using deep learning transformer architectures 使用深度学习转换器架构增强特定站点的杂草检测
IF 2.8 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-12-12 DOI: 10.1016/j.cropro.2024.107075
Francisco Garibaldi-Márquez, Daniel A. Martínez-Barba, Luis E. Montañez-Franco, Gerardo Flores, Luis M. Valentín-Coronado
Detection of weeds is essential to implement an intelligent weed control system in natural corn fields. Then, to address this issue, the Swin-UNet, Segmenter, and SegFormer deep learning transformer architectures have been implemented and compared. Furthermore, a simple thresholding method has been performed to enhance the segmentation. Moreover, a large pixel-level annotated image dataset acquired under natural field conditions is introduced to train the models. In addition, the well-known Precision, Dice Similarity Coefficient (DSC), Intersection over Union (IoU), and mean Intersection over Union (mIoU) metrics have been used to evaluate the implemented models’ performance. According to the experimental results, the SegFormer architecture was the best model on each of the three proposed weed detection approaches, achieving a macro performance of up to 94.49%, 95.30%, and 91.26% for Precision, DSC, and mIoU, respectively.
检测杂草对于在天然玉米田中实施智能杂草控制系统至关重要。为解决这一问题,我们采用了 Swin-UNet、Segmenter 和 SegFormer 深度学习变换器架构,并对其进行了比较。此外,还采用了一种简单的阈值处理方法来增强分割效果。此外,还引入了在自然野外条件下获取的大型像素级注释图像数据集来训练模型。此外,还使用了著名的精度、骰子相似系数(DSC)、联合交叉(IoU)和平均联合交叉(mIoU)指标来评估所实现模型的性能。实验结果表明,SegFormer 架构在三种杂草检测方法中都是最佳模型,其精确度、DSC 和 mIoU 的宏观性能分别高达 94.49%、95.30% 和 91.26%。
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引用次数: 0
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Crop Protection
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