Brazil is a leading producer and exporter of Galia melon (Cucumis melo L.), a variety renowned for its exceptional sensory qualities. However, postharvest diseases caused by Fusarium spp. Lead to significant economic losses, estimated at up to US$22 million annually. The current reliance on fungicides, such as Imazalil, is unsustainable due to regulatory restrictions and the increasing fungal resistance to current chemistry, which calls for the development of alternative control strategies. In this study, we investigated the effectiveness of thermotherapy combined with ripening phytoregulators for managing rots caused by F. falciforme and F. sulawesiense in Galia melons. Inoculated fruits underwent eight different treatments [Mock (control), HWT (hot water treatment), 1-MCP (1-methylcyclopropene), HWT + 1-MCP, GA (gibberellin), HWT + GA, GRAD (Graduate A+®), and HWT + GRAD] and were stored for 20 days at 10 ± 2 °C. We evaluated disease severity, melon fruit physicochemical parameters, and the activity of plant defense enzymes. Most treatments significantly reduced rot severity compared to the control. Notably, Graduate A+® achieved up to a 72.9 % reduction, and its efficacy was enhanced when combined with thermotherapy. Furthermore, 1- methylcyclopropene (1-MCP) treatment, alone and with thermotherapy, preserved postharvest quality and enhanced defense-related enzyme activity. These findings suggest that the combination of thermotherapy and 1-MCP represents a promising and sustainable approach to reducing Fusarium rot in Galia melons destined for export, with no associated regulatory risks.
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