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Small-sample cucumber disease identification based on multimodal self-supervised learning 基于多模态自监督学习的小样本黄瓜病害识别
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-30 DOI: 10.1016/j.cropro.2024.107006
Yiyi Cao , Guangling Sun , Yuan Yuan , Lei Chen
It is difficult and costly to obtain large-scale, labeled crop disease data in the field of agriculture. How to use small samples of unlabeled data for feature learning has become an urgent problem that needs to be solved. The emergence of self-supervised contrastive learning methods and self-supervised mask learning methods can solve the problem of missing labels on the training data. However, each of these paradigms comes with its own advantages and drawbacks. At the same time, the features learned by dataset in a single modality are limited, ignoring the correlation with other modal information. Hence, this paper introduced an effective framework for multimodal self-supervised learning, denoted as MMSSL, to address the task of identifying cucumber diseases with small sample sizes. Integrating image self-supervised mask learning, image self-supervised contrastive learning, and multimodal image-text contrastive learning, the model can not only learn disease feature information from different modalities, but also capture global and local disease feature information. Simultaneously, the mask learning branch was enhanced by introducing a prompt learning module based on a cross-attention network. This module aided in approximately locating the masked regions in the image data in advance, facilitating the decoder in making accurate decoding predictions. Experimental results demonstrate that the proposed method achieves a 95% accuracy in cucumber disease identification in the absence of labels. The approach effectively uncovers high-level semantic features within multimodal small-sample cucumber disease data. GradCAM is also employed for visual analysis to further understand the decision-making process of the model in disease identification. In conclusion, the proposed method in this paper is advantageous for enhancing the classification accuracy of small-sample cucumber data in a multimodal, unlabeled context, demonstrating good generalization performance.
在农业领域,获取大规模、有标记的作物病害数据既困难又昂贵。如何利用未标记的小样本数据进行特征学习已成为亟待解决的问题。自监督对比学习方法和自监督掩码学习方法的出现可以解决训练数据中标签缺失的问题。然而,这些范式各有利弊。同时,单一模态数据集学习到的特征是有限的,忽略了与其他模态信息的相关性。因此,本文介绍了一种有效的多模态自监督学习框架(简称 MMSSL),以解决样本量较小的黄瓜病害识别任务。该模型集成了图像自监督掩码学习、图像自监督对比学习和多模态图像-文本对比学习,不仅能学习不同模态的疾病特征信息,还能捕捉全局和局部疾病特征信息。同时,通过引入基于交叉注意网络的提示学习模块,掩膜学习分支得到了增强。该模块有助于提前在图像数据中大致定位掩码区域,从而帮助解码器做出准确的解码预测。实验结果表明,所提出的方法在没有标签的情况下识别黄瓜疾病的准确率达到 95%。该方法有效地发现了多模态小样本黄瓜疾病数据中的高级语义特征。此外,还利用 GradCAM 进行了可视化分析,以进一步了解该模型在疾病识别中的决策过程。总之,本文提出的方法有利于提高多模态、无标记背景下黄瓜小样本数据的分类准确性,并表现出良好的泛化性能。
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引用次数: 0
Labor-saving application of thifluzamide and tricyclazole to seedling trays for integrated control of rice blast and sheath blight 在秧盘中施用噻虫嗪和三环唑综合防治稻瘟病和鞘枯病,节省劳动力
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-29 DOI: 10.1016/j.cropro.2024.107004
Xugen Shi , Kang Qiao , Yong Zhang , Shouan Zhang , Yong Liu , Xianpeng Zhang , Baotong Li , Ruqiang Cui
Rice blast (Magnaporthe grisea) and sheath blight (Rhizoctonia solani) are limiting factors for rice production. Co-infection of these pathogens results in a disease complex which is difficult to control. In China, growers are accustomed to applying individual fungicides to manage blast and sheath blight, which require large amounts of labor. Application to seedling trays is a new promising solution for saving labor. In this study, application of combined fungicides with different modes of action to seedling trays to integrate control rice blast and sheath blight was evaluated in vitro assays and field trials. The results showed that the combination of thifluzamide and tricyclazole in the 1:2 ratio had significant synergistic inhibitory effects on the mycelial growth of M. grisea and R. solani, with a synergistic ratio (SR) of 2.17 and 1.49. Results from field trials revealed that thifluzamide + tricyclazole at 1107 and 958.5 g/ha applied to seedling trays was the most effective treatment to reduce the disease index of rice blast with control effects of 83.74–84.96% and 81.34–83.26% in 2022 and 2023, respectively, and no significant differences were observed from tricyclazole at 300 g/ha as foliar sprays twice. Compared to the untreated control, disease index of rice sheath blight was notably reduced by all treatments containing thifluzamide. The highest control was recorded in the treatment of thifluzamide + tricyclazole applied at 1107 g/ha to seedling trays. Moreover, compared to the untreated control, all treatments significantly enhanced rice grain yield by 7.67–17.86% and 3.38–18.91% in 2022 and 2023, respectively. The greatest yield (7429.73 and 7404.73 kg/ha in 2022 and 2023, respectively) was observed from the treatment of thifluzamide + tricyclazole at 1107 g/ha applied to seedling trays, with no significant differences among all the treatments containing tricyclazole. Taken together, these results indicated that seedling tray application of thifluzamide + tricyclazole could be a labor-saving approach to the integrated control of rice blast and sheath blight disease complex, while increasing rice grain yield.
稻瘟病(Magnaporthe grisea)和鞘枯病(Rhizoctonia solani)是限制水稻产量的因素。这些病原体的共同侵染导致了难以控制的复合病害。在中国,种植者习惯于使用单独的杀菌剂来防治稻瘟病和鞘枯病,这需要大量的劳动力。在育苗盘中施用杀菌剂是一种节省劳动力的新型解决方案。本研究在体外试验和田间试验中评估了在秧盘中施用不同作用模式的复合杀菌剂来综合防治稻瘟病和鞘枯病的效果。结果表明,噻虫嗪和三环唑以 1:2 的比例复配,对 M. grisea 和 R. solani 的菌丝生长有显著的增效抑制作用,增效比(SR)分别为 2.17 和 1.49。田间试验结果表明,噻虫嗪+三环唑1107克/公顷和958.5克/公顷施用于秧盘是降低稻瘟病发病指数最有效的处理方法,在2022年和2023年的防治效果分别为83.74-84.96%和81.34-83.26%,与三环唑300克/公顷叶面喷施两次的防治效果无显著差异。与未处理的对照相比,含氟虫酰胺的所有处理都显著降低了水稻鞘枯病的发病指数。对秧盘施用 1107 克/公顷的噻虫胺+三环唑处理的防治效果最高。此外,与未处理的对照相比,所有处理都显著提高了 2022 年和 2023 年的稻谷产量,增幅分别为 7.67-17.86% 和 3.38-18.91%。在秧盘中施用 1107 克/公顷的噻虫嗪+三环唑处理的产量最高(2022 年和 2023 年分别为 7429.73 千克/公顷和 7404.73 千克/公顷),而含有三环唑的所有处理之间没有显著差异。总之,这些结果表明,秧盘施用噻虫胺+三环唑是一种省力的方法,可综合防治稻瘟病和鞘病复合病害,同时提高水稻产量。
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引用次数: 0
EQID: Entangled quantum image descriptor an approach for early plant disease detection EQID:纠缠量子图像描述符--植物早期病害检测方法
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-29 DOI: 10.1016/j.cropro.2024.107005
Ishana Attri, Lalit Kumar Awasthi (Prof) , Teek Parval Sharma
In present day agriculture, early and accurate identification of plant diseases is essential for prompt response, which protects crop quality and output. This paper presents the Entangled Quantum-Inspired Deep learning model (EQID), a unique method that improves feature representation and classification in plant disease prediction by utilizing the concepts of quantum computing. Two different datasets with images of potatoes and tomatoes as leaves were used to test the EQID model, which performed better than traditional models. EQID obtained 98.96% accuracy, 98.98% precision, 98.96% recall, and 98.90% F1 score on images of potato leaves. For tomato leaves, comparable outcomes were noted, with accuracy, precision, recall, and F1 score all above 99.61%. The accuracy of disease prediction is greatly increased by the efficient and effective feature representation made possible by the EQID model's inclusion of quantum computing techniques. Additionally, the model outperformed other cutting-edge models such as DenseNet-121, VGGNet 16, and Xception Net, illustrating the potentially revolutionary effects of quantum-inspired models in agriculture. Future work will focus on applying the EQID model to a broader range of crops and plant diseases, as well as incorporating additional data sources to further enhance the model's predictive capabilities.
在当今农业领域,及早准确地识别植物病害对于及时采取应对措施、保护作物质量和产量至关重要。本文介绍了纠缠量子启发深度学习模型(EQID),这是一种利用量子计算概念改进植物病害预测中特征表示和分类的独特方法。EQID 模型使用了两个不同的数据集,分别以马铃薯和西红柿的叶片为图像进行测试,其表现优于传统模型。EQID 在马铃薯叶片图像上获得了 98.96% 的准确率、98.98% 的精确率、98.96% 的召回率和 98.90% 的 F1 分数。在番茄叶片上,准确率、精确率、召回率和 F1 分数均高于 99.61%,结果与之相当。EQID 模型采用量子计算技术,实现了高效的特征表示,从而大大提高了疾病预测的准确性。此外,该模型的性能还优于 DenseNet-121、VGGNet 16 和 Xception Net 等其他前沿模型,这说明量子启发模型在农业领域具有潜在的革命性影响。未来的工作重点是将 EQID 模型应用到更广泛的作物和植物病害中,并纳入更多数据源,以进一步增强模型的预测能力。
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引用次数: 0
Few-shot agricultural pest recognition based on multimodal masked autoencoder 基于多模态掩码自动编码器的农业害虫识别技术
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-28 DOI: 10.1016/j.cropro.2024.106993
Yinshuo Zhang , Lei Chen , Yuan Yuan
Visual recognition methods based on deep convolutional neural networks have performed well in pest diagnosis and have gradually become a research hotspot. However, agricultural pest recognition faces challenges such as few-shot learning, category imbalance, similarity in appearance, and small pest targets. Existing deep learning-based pest recognition methods typically rely solely on unimodal image data, which results in a model whose recognition performance is heavily dependent on the size and quality of the annotated training dataset. However, the construction of large-scale, high-quality pest datasets requires significant economic and technical costs, limiting the practical generalization of existing methods for pest recognition. To address these challenges, this paper proposes a few-shot pest recognition model called MMAE (multimodal masked autoencoder). Firstly, the masked autoencoder of MMAE integrates self-supervised learning, which can be applied to few-shot datasets and improves recognition accuracy. Secondly, MMAE embeds textual modal information on top of image modal information, thus improving the performance of pest recognition by utilizing the correlation and complementarity between the two modalities. The experimental results show that MMAE is the most effective for pest identification compared with the existing excellent models, and the identification accuracy is as high as 98.12%, which is 1.61 percentage points higher than the current state-of-the-art MAE method. The work in this paper shows that the introduction of textual information can assist the visual coder in capturing agricultural pest characterization information at a higher level of granularity, providing a methodological reference for solving the problem of agricultural pest recognition under few-shot conditions.
基于深度卷积神经网络的视觉识别方法在害虫诊断中表现出色,逐渐成为研究热点。然而,农业害虫识别面临着学习次数少、类别不平衡、外观相似性和害虫目标小等挑战。现有的基于深度学习的害虫识别方法通常仅依赖于单模态图像数据,这导致模型的识别性能严重依赖于标注训练数据集的规模和质量。然而,构建大规模、高质量的害虫数据集需要大量的经济和技术成本,这限制了现有害虫识别方法的实际推广。为了应对这些挑战,本文提出了一种名为 MMAE(多模态掩蔽自动编码器)的少量害虫识别模型。首先,MMAE 的遮蔽自编码器集成了自监督学习,可应用于少量数据集并提高识别准确率。其次,MMAE 在图像模态信息的基础上嵌入了文本模态信息,从而利用两种模态之间的相关性和互补性提高了虫害识别的性能。实验结果表明,与现有的优秀模型相比,MMAE 是最有效的害虫识别方法,识别准确率高达 98.12%,比目前最先进的 MAE 方法高出 1.61 个百分点。本文的工作表明,文本信息的引入可以帮助视觉编码器捕捉到更高粒度的农业害虫特征信息,为解决少镜头条件下的农业害虫识别问题提供了方法参考。
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引用次数: 0
Early plant disease detection by Raman spectroscopy: An open-source software designed for the automation of preprocessing and analysis of spectral dataset 利用拉曼光谱进行早期植物病害检测:为自动预处理和分析光谱数据集而设计的开源软件
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-28 DOI: 10.1016/j.cropro.2024.107003
Moisés R. Vallejo Pérez , Juan J. Cetina Denis , Mariana A. Chan Ley , Jesús A. Sosa Herrera , Juan C. Delgado Ortiz , Ángel G. Rodríguez Vázquez , Hugo R. Navarro Contreras
This study introduces a reliable, non-coding software named qREAD-Raman, written in the JavaScript® language, for analyzing and interpreting Raman spectral information. It is designed with a focus on the early detection of diseases in tomato plants (S. lycopersicum) during the asymptomatic stage. The platform integrates a set of machine learning algorithms necessary for the preprocessing consisting of outlier removal, baseline correction, fluorescence removal, smoothing, and normalization. For classification, we applied a Consensus of five different classifiers: Multilayer Perceptron (MLP), Partial Least Squares-Discriminant Analysis (PLS-DA), Linear Discriminant Analysis (LDA), Long Short-Term Memory (LSTM), and K-nearest neighbors (kNN). The experiments were conducted on two bacterial diseases: bacterial canker of tomato induced by Clavibacter michiganesis subsp. michiganensis (Cmm), and the tomato vein-greening associated with Candidatus Liberibacter solanacearum (CLso), a non-culturable bacteria transmitted by Bactericera cockerelli insect. Binary models (Cmm-Healthy and CLso-Healthy) demonstrated excellent classification ability. Asymptomatic Cmm-infected plants were distinguished with an accuracy of 88–95 %, while CLso-infected plants showed an accuracy of 68–77 %. The three-class model (CLso-Cmm-Healthy) exhibited acceptable performance in differentiating between Cmm and CLso, with accuracy rates of 71–83% and 58–67%, respectively. The model's performance highlights differences in the relevant spectral regions associated with the biochemical changes induced by each studied disease. The qREAD-Raman software, implemented for the purpose of this research, was found to be a valuable and comprehensive tool that effectively differentiate diseased tomato plants during their asymptomatic stage.
本研究介绍了一种可靠的非编码软件 qREAD-Raman,它是用 JavaScript® 语言编写的,用于分析和解释拉曼光谱信息。该软件主要用于番茄植物(S. lycopersicum)无症状阶段的早期病害检测。该平台集成了一套必要的机器学习算法,用于预处理,包括离群点去除、基线校正、荧光去除、平滑和归一化。在分类方面,我们应用了五种不同分类器的共识:多层感知器 (MLP)、部分最小二乘判别分析 (PLS-DA)、线性判别分析 (LDA)、长短期记忆 (LSTM) 和 K 最近邻 (kNN)。实验针对两种细菌性病害进行:由密歇根氏棒状杆菌亚种(Cmm)诱发的番茄细菌性腐烂病,以及由鸡冠霉菌(Bactericera cockerelli)昆虫传播的一种不可培养的细菌--茄自由杆菌(CLso)引起的番茄脉绿病。二元模型(Cmm-健康和 CLso-健康)显示了出色的分类能力。区分无症状 Cmm 感染植物的准确率为 88-95%,而 CLso 感染植物的准确率为 68-77%。三类模型(CLso-Cmm-Healthy)在区分 Cmm 和 CLso 方面的表现尚可,准确率分别为 71-83% 和 58-67%。该模型的性能突出显示了与所研究的每种疾病引起的生化变化相关的光谱区域的差异。为本研究目的而实施的 qREAD-Raman 软件被认为是一种有价值的综合工具,能在番茄无症状阶段有效地区分患病的番茄植株。
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引用次数: 0
Cassava crop disease prediction and localization using object detection 利用物体检测进行木薯作物病害预测和定位
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-26 DOI: 10.1016/j.cropro.2024.107001
Josephat Kalezhi, Langtone Shumba
In agriculture, early detection and localization of plant diseases in time using deep learning techniques can help farmers contain the spread of plant diseases. In this work, we apply object detection models to identify and localize various categories of cassava plant leaf diseases. These include You Only Look Once (YOLO) as well as Generalized Efficient Layer Aggregation Network(GELAN) models. We applied YOLO v9-e, YOLO v9-c, as well as GELAN-e and GELAN-c models. The models were successfully trained using a custom cassava dataset. Several evaluation indicators that include precision, recall and mean average precision(mAP) were analysed result. The results have been compared with an earlier version of YOLO model and show an improvement in evaluation indicators reaching above 80% in the majority of diseases.
在农业领域,利用深度学习技术及时对植物病害进行早期检测和定位,可以帮助农民遏制植物病害的蔓延。在这项工作中,我们应用对象检测模型来识别和定位各类木薯植物叶片病害。这些模型包括 "只看一次"(YOLO)模型和广义高效层聚合网络(GELAN)模型。我们应用了 YOLO v9-e、YOLO v9-c,以及 GELAN-e 和 GELAN-c 模型。我们使用定制的木薯数据集成功地训练了这些模型。结果分析了多个评估指标,包括精确度、召回率和平均精确度(mAP)。将结果与早期版本的 YOLO 模型进行了比较,结果表明,在大多数病害中,评估指标的改进幅度超过了 80%。
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引用次数: 0
High-efficiency fungicide screening and field control efficacy of maize southern corn rust 玉米南方锈病的高效杀菌剂筛选和田间防治效果
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-24 DOI: 10.1016/j.cropro.2024.106997
Yigeng Wang , Yifei Shao , Xiaoyan Zhao , Qinghui Pei , Ning Nan , Fengwen Zhang , Xingyin Jiang , Xiangdong Li
Southern corn rust caused by Puccinia dothidea is becoming one of the main diseases of maize planting in China, which seriously threatens the yield of maize in China. Currently, there is no registered pesticide to control southern corn rust in China. Therefore, it is urgent to screen out efficient pesticides to control southern corn rust and reduce the harm of multistem rust to maize yield in China. In the present study, the indoor virulence determination of spore-germination method and 2 years of field efficacy tests were used to screen the fungicides with good prevention and control efficacy on maize southern rust. The results of the indoor toxicity test in 2023 showed that the EC50 of P. dothidea to benzoflumizole was 0.0085 mg/L, and the sensitivity to thifluzamide was relatively poor, with an EC50 of 1.2585 mg/L. The mixture of pyraclostrobin and benzoflumizole at a ratio of 4:1 had the optimal synergistic effect, with an EC50 of 0.0181 mg/L and a cotoxicity coefficient of 128.36. The results of the field efficacy test revealed that 60 g/ha benzovinfluconazole had a good field control effect, and the control effect was 83.09%–85.95%. The combination of 30 g/ha benzodifluconazole and 93.75 g/ha pyraclostrobin had the optimal control effect, and the control effect was 86.92%–88.58% and safe for corn. The results indicated that the application of benzovindiflupyr and its mixture with pyraclostrobin had a good effect on the prevention and control of southern corn rust, which could provide a theoretical basis for the prevention and control of southern corn rust in production and scientific medication.
由 Puccinia dothidea 引起的南方玉米锈病正在成为中国玉米种植的主要病害之一,严重威胁着中国玉米的产量。目前,中国还没有登记防治南方玉米锈病的农药。因此,筛选出防治南方玉米锈病的高效农药,降低多茎锈病对我国玉米产量的危害迫在眉睫。本研究采用孢子萌发法室内毒力测定和2年田间药效试验,筛选出对玉米南方锈病具有良好防治效果的杀菌剂。2023 年室内毒力试验结果表明,多锈菌对苯并福美唑的 EC50 为 0.0085 mg/L,对噻虫嗪的敏感性相对较差,EC50 为 1.2585 mg/L。吡唑醚菌酯和苯并氟咪唑以 4:1 的比例混合使用,具有最佳的增效作用,EC50 为 0.0181 毫克/升,毒性系数为 128.36。田间药效试验结果表明,60 克/公顷苯醚甲环唑具有良好的田间防治效果,防治效果为 83.09%-85.95%。苯醚甲环唑 30 克/公顷和吡唑醚菌酯 93.75 克/公顷的组合防治效果最佳,防治效果为 86.92%-88.58%,对玉米安全。结果表明,施用苯醚甲环唑及其与吡唑醚菌酯的混剂对南方玉米锈病有较好的防治效果,可为生产上防治南方玉米锈病和科学用药提供理论依据。
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引用次数: 0
Phenotypic characterization of advanced cotton lines for resistance to defoliating pathotype of Verticillium dahliae 先进棉花品系抗大丽轮枝菌落叶病型的表型特征
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-24 DOI: 10.1016/j.cropro.2024.107000
Mehmet Aydoğdu , Metin Durmuş Çetin , Selfinaz Kübra Velioğlu , İlker Kurbetli
Verticillium wilt caused by Verticillium dahliae is one of the most devastating diseases of cotton worldwide but little is known about resistance of cotton to V. dahliae for phenotypic quantifying. The aim of the current study was to phenotypically characterize resistance of 18 advanced cotton lines to defoliating pathotype (Vd-34) of V. dahliae. Experiments were set up in a greenhouse in two consecutive years. Phenotyping of plant response to V. dahliae was quantified by assessing five agronomic traits (defoliation rate, plant height, root weight, stem diameter, biomass) and internal vascular discoloration. Reaction types were established using area under the disease progress curve (AUDPC). The defoliating pathotype Vd-34 induced significant (P ˂ 0.01) reductions in all the examined agrononic traits. Overall reductions in root weight, defoliation rate, plant height, biomass and stem diameter were detected as 42.33, 34.13, 33.56, 32.31 and 26.63%, respectively. Based on the AUDPC values, of the 18 advanced cotton lines, AntV-17, AntV-19 and AntV-20 were detected as moderately resistant to the defoliating pathotype of V. dahliae, while the others showed moderately susceptible, susceptible and very susceptible reactions. The disease quantifying parameters (AUDPC, rAUDPC and vascular disease score) significantly (P ˂ 0.01) and positively correlated with the reductions in the examined agronomic traits. To our knowledge, this is the first detailed phenotyping for resistance to defoliating pathotype of V. dahliae in cotton and reveals new knowledge to quantify cotton resistance to V. dahliae.
由大丽轮枝菌(Verticillium dahliae)引起的轮枝枯萎病是全球棉花最具毁灭性的病害之一,但人们对棉花对大丽轮枝菌抗性的表型量化知之甚少。本研究旨在从表型上描述 18 个先进棉花品系对大丽轮枝菌脱叶病型(Vd-34)的抗性。实验连续两年在温室中进行。通过评估五个农艺性状(落叶率、株高、根重、茎直径、生物量)和内部维管变色,对植物对大丽蚜的反应进行表型量化。通过病害进程曲线下面积(AUDPC)确定反应类型。脱叶病原型 Vd-34 导致所有受检农艺性状显著降低(P ˂ 0.01)。根重、落叶率、株高、生物量和茎径的总体下降率分别为 42.33%、34.13%、33.56%、32.31% 和 26.63%。根据 AUDPC 值,在 18 个先进棉花品系中,AntV-17、AntV-19 和 AntV-20 被检测出对大丽花落叶病病原型具有中度抗性,而其他品系则表现出中度感病、感病和极感病反应。病害量化参数(AUDPC、rAUDPC 和维管束病害评分)与所考察的农艺性状的减少呈显著正相关(P ˂ 0.01)。据我们所知,这是首次对棉花对大丽花病毒(V. dahliae)落叶病病原型的抗性进行详细的表型分析,揭示了量化棉花对大丽花病毒(V. dahliae)抗性的新知识。
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引用次数: 0
Surveys of virus diseases and molecular identification of viruses affecting pepper crops (Capsicum spp.) in southern Benin 贝宁南部辣椒作物(辣椒属)病毒病调查和病毒分子鉴定
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-24 DOI: 10.1016/j.cropro.2024.106999
Antoine Abel Missihoun , André Antoine Fanou , Chimène Nadège Mahoussi Nanoukon , Ignace Relique Agbo , Paulin Sedah , Mongane Fays , Nicolas Desoignies
Boosting the production of market garden products is a top priority for agricultural development in Benin. Chili pepper (Capsicum spp.), an economically important spice, is widely cultivated for its fruits in different parts of the country. Viral diseases are known to have a devastating impact on production. The objective of this study was to identify the viruses associated with pepper cultivation in southern Benin. Surveys and sample collections were conducted in five districts (Ouidah, Kpomassè, Sèmè, Cotonou, and Abomey-Calavi) from three departments (Atlantic, Littoral, and Ouémè) in September 2021. Disease incidence and severity of infection were assessed. Leaf samples from symptomatic and asymptomatic plants were collected randomly from farmers’ fields and were analyzed for viruses by RT-PCR using twelve primer pairs for different viruses sought. The overall high incidence of diseases varied according to the localities between 84.76% and 100% with an average of 94.46%. As for the severity of the disease, it varied significantly depending on the location of 3.24 (in Sèmè-Kpodji) to 2.56 (in Ouidah Cotonou) on average. The analysis of the data obtained by the detection of the presence of viruses in the various samples by RT-PCR shows the following occurrences: potato virus X (PVX) 77.36%, potato virus Y veinal necrosis strain (PVYn) 52.83%, cucumber mosaic virus (CMV) 47.17%, pepper vein virus (PeVYV) 32.08%, potato yellow mosaic virus (PYMV) 32.08%, Polerovirus 32.08%, Begomovirus 32.08%, tomato spoon yellow leaf (TYLCV) 18.87%, tomato spotted wilt virus (TSWV) 15.09%, pepper mottle virus (PepMoV) 11.32%, pepper mild mottle virus (PMMoV) 03.77%, and PVY 0.00% in the five districts surveyed during the periods under survey. Of the twelve groups of viruses searched for in the samples, eleven were found. Each of the samples is infected with one to seven of the twelve viruses sought. Mixed infections were common in most samples, and the high incidence suggests that the cultivars are highly susceptible to viral infections.
促进市场园艺产品的生产是贝宁农业发展的重中之重。辣椒(辣椒属)是一种具有重要经济价值的香料,因其果实而在贝宁各地广泛种植。众所周知,病毒性疾病会对生产造成破坏性影响。本研究旨在确定与贝宁南部辣椒种植相关的病毒。2021 年 9 月,在三个省(大西洋省、滨海省和韦梅省)的五个地区(维达、克波马塞、塞梅、科托努和阿波美-卡拉维)进行了调查和样本采集。对病害发生率和感染严重程度进行了评估。有症状和无症状植株的叶片样本从农民田间随机采集,并通过 RT-PCR 分析病毒,使用 12 对引物对所寻找的不同病毒进行分析。不同地区的总体高发病率在 84.76% 到 100% 之间,平均为 94.46%。至于疾病的严重程度,各地的差异很大,平均为 3.24(塞梅-克波吉)到 2.56(维达-科托努)。通过 RT-PCR 检测各种样本中是否存在病毒所获得的数据分析显示,病毒发生率如下:马铃薯病毒 X(PVX)77.36%、马铃薯病毒 Y 脊髓坏死株(PVYn)52.83%、黄瓜花叶病毒(CMV)47.17%、辣椒叶脉病毒(PeVYV)32.在所调查的五个地区中,黄瓜花叶病毒(CMV)占 52.83%,黄瓜花叶病毒(CMV)占 47.17%,辣椒细脉病毒(PeVYV)占 32.08%,马铃薯黄花叶病毒(PYMV)占 32.08%,多角体病毒(Polerovirus)占 32.08%,Begomovirus 占 32.08%,番茄匙形黄叶病毒(TYLCV)占 18.87%,番茄斑萎病毒(TSWV)占 15.09%,辣椒斑驳病毒(PepMoV)占 11.32%,辣椒轻度斑驳病毒(PMMoV)占 03.77%,PVY 占 0.00%。在样本中搜索的 12 组病毒中,发现了 11 组。每个样本都感染了 12 种病毒中的 1 至 7 种。混合感染在大多数样本中很常见,高发生率表明栽培品种极易受到病毒感染。
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引用次数: 0
First report of cucurbit yellow stunting disorder virus causing yellowing disease on major gourds in India 首次报告葫芦黄化病病毒在印度主要瓜类上引起黄化病
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-24 DOI: 10.1016/j.cropro.2024.106998
B.S. Bharath , K. Nagendran , S. Harish , G. Karthikeyan
Cucurbitaceous crops are a key group of vegetables widely cultivated in India. Characterized yellowing symptoms along with interveinal chlorosis, mottling and downward curling were observed in ridge gourd, snake gourd, and bottle gourd along with the presence of whiteflies (Bemisia tabaci). To investigate the infecting virus, RT-PCR assays followed by sequencing and Transmission Electron Microscopy (TEM) analysis were performed. Sanger sequencing, nucleotide sequence analysis, and TEM imaging confirmed the association of cucurbit yellow stunting disorder virus (CYSDV), genus Crinivirus within the family Closteroviridae. This study reports the natural occurrence of CYSDV in ridge gourd, snake gourd, and bottle gourd for the first time in India.
葫芦科作物是印度广泛种植的一类重要蔬菜。在脊瓠瓜、蛇瓠瓜和瓶瓠瓜上观察到了特征性的黄化症状以及叶脉间萎黄、斑驳和向下卷曲,同时还发现了粉虱(Bemisia tabaci)。为了研究感染病毒,进行了 RT-PCR 检测,然后进行了测序和透射电子显微镜(TEM)分析。桑格测序、核苷酸序列分析和透射电子显微镜成像证实了葫芦黄矮病病毒(CYSDV)与瘤胃病毒科瘤胃病毒属(Crinivirus)的关系。本研究首次报道了 CYSDV 在印度脊瓜、蛇瓜和瓶瓜中的自然发生。
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引用次数: 0
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Crop Protection
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