首页 > 最新文献

Crop Protection最新文献

英文 中文
Identification of tomato leaf diseases based on DGP-SNNet 基于 DGP-SNNet 的番茄叶片病害鉴定
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-05 DOI: 10.1016/j.cropro.2024.106975
Existing deep learning techniques for tomato leaf disease recognition face several challenges, including external environmental interference, limited dataset size, imbalanced sample distribution, and overlapping characteristics among different diseases, which complicate accurate disease identification. Furthermore, complex models with a high number of parameters are often difficult to deploy on resource-constrained embedded devices. To address these challenges, this paper proposes a novel tomato leaf disease recognition method based on DGP-SNNet. Initially, to mitigate issues related to imbalanced samples and overfitting, we introduce a two-stage transfer learning technique alongside a partial convolution module (PConv) to decrease data dependency and enhance model stability. Subsequently, we propose a Global Grouped Location Attention (GGLA) mechanism that dynamically adapts to capture fine-grained disease information, thereby addressing the similarities between disease categories. Finally, we employ a joint compression method utilizing Network Slimming and Neuron Selectivity Transfer, which significantly reduces model size with minimal loss in accuracy. Experimental results demonstrate a classification accuracy of 99.55%, with FLOPs of 1011.88 MB and a parameter count of 4.93 MB. Compared to the baseline model, accuracy improved by 2.23%, FLOPs decreased by 63.39%, and the parameter count was reduced by 75.13%. Additionally, we achieved optimal performance through comparative analyses with other classical and state-of-the-art models, generalization experiments, and module effectiveness tests. In conclusion, the proposed method effectively recognizes various diseases in tomato leaves and offers a practical solution for the integration of deep learning into agricultural production processes.
现有的番茄叶片疾病识别深度学习技术面临着一些挑战,包括外部环境干扰、数据集规模有限、样本分布不平衡以及不同疾病之间的特征重叠,这些都使疾病的准确识别变得复杂。此外,具有大量参数的复杂模型往往难以在资源有限的嵌入式设备上部署。为应对这些挑战,本文提出了一种基于 DGP-SNNet 的新型番茄叶病识别方法。首先,为了缓解与样本不平衡和过拟合相关的问题,我们引入了两阶段迁移学习技术和部分卷积模块(PConv),以降低数据依赖性并增强模型稳定性。随后,我们提出了全局分组位置关注(GGLA)机制,该机制可动态调整以捕捉细粒度疾病信息,从而解决疾病类别之间的相似性问题。最后,我们采用了一种利用网络瘦身和神经元选择性转移的联合压缩方法,该方法在大幅减小模型大小的同时将准确性损失降至最低。实验结果表明,分类准确率为 99.55%,FLOP 为 1011.88 MB,参数数为 4.93 MB。与基线模型相比,准确率提高了 2.23%,FLOPs 减少了 63.39%,参数数量减少了 75.13%。此外,我们还通过与其他经典模型和最先进模型的对比分析、泛化实验和模块有效性测试,实现了最佳性能。总之,所提出的方法能有效识别番茄叶片上的各种病害,为将深度学习融入农业生产流程提供了一种实用的解决方案。
{"title":"Identification of tomato leaf diseases based on DGP-SNNet","authors":"","doi":"10.1016/j.cropro.2024.106975","DOIUrl":"10.1016/j.cropro.2024.106975","url":null,"abstract":"<div><div>Existing deep learning techniques for tomato leaf disease recognition face several challenges, including external environmental interference, limited dataset size, imbalanced sample distribution, and overlapping characteristics among different diseases, which complicate accurate disease identification. Furthermore, complex models with a high number of parameters are often difficult to deploy on resource-constrained embedded devices. To address these challenges, this paper proposes a novel tomato leaf disease recognition method based on DGP-SNNet. Initially, to mitigate issues related to imbalanced samples and overfitting, we introduce a two-stage transfer learning technique alongside a partial convolution module (PConv) to decrease data dependency and enhance model stability. Subsequently, we propose a Global Grouped Location Attention (GGLA) mechanism that dynamically adapts to capture fine-grained disease information, thereby addressing the similarities between disease categories. Finally, we employ a joint compression method utilizing Network Slimming and Neuron Selectivity Transfer, which significantly reduces model size with minimal loss in accuracy. Experimental results demonstrate a classification accuracy of 99.55%, with FLOPs of 1011.88 MB and a parameter count of 4.93 MB. Compared to the baseline model, accuracy improved by 2.23%, FLOPs decreased by 63.39%, and the parameter count was reduced by 75.13%. Additionally, we achieved optimal performance through comparative analyses with other classical and state-of-the-art models, generalization experiments, and module effectiveness tests. In conclusion, the proposed method effectively recognizes various diseases in tomato leaves and offers a practical solution for the integration of deep learning into agricultural production processes.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sublethal effects of emamectin benzoate on biological characteristics and the expression of vitellogenin (SgVg) and its receptor in Sclerodermus guani 苯甲酸阿维菌素对硬皮豚鼠卵黄素(SgVg)及其受体的生物特征和表达的亚致死效应
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-04 DOI: 10.1016/j.cropro.2024.106971
Emamectin benzoate (EMB) is a commonly used insecticide for controlling agricultural and forest pests. To facilitate its rational use in integrated pest management (IPM), the sublethal effects of EMB on the development and reproduction of Scleroderma guani were evaluated. Sublethal doses of EMB (LD10 and LD30) significantly reduced the longevity and fecundity of F0 females. In the F1 generation, sublethal doses significantly reduced larval development duration and male longevity but significantly extended female longevity. Additionally, the LD30 treatment significantly decreased the number of laid eggs in F1 females by 20.72% compared with the control group. The net reproductive rate (R0) was significantly declined by the LD30 treatment compared with the control group (CK). Furthermore, the mean generation time (T) was significantly extended in both LD10 and LD30 treatment groups. Compared to the control group, the expression of SgVg and SgVgR genes in the LD10 and LD30 groups was significantly lower, which may be a crucial factor for the reduced fecundity of the females. These results indicate that sublethal doses of EMB significantly reduced adult longevity and fecundity of S. guani. The findings are practical for developing IPM strategies for forestry pests using S. guani and EMB.
苯甲酸阿维菌素(EMB)是一种常用的杀虫剂,用于控制农业和森林害虫。为促进其在害虫综合治理(IPM)中的合理使用,研究人员评估了 EMB 对瓜氏硬皮虫发育和繁殖的亚致死效应。亚致死剂量的 EMB(LD10 和 LD30)显著降低了 F0 代雌虫的寿命和繁殖力。在 F1 代中,亚致死剂量显著缩短了幼虫发育时间和雄性寿命,但显著延长了雌性寿命。此外,与对照组相比,LD30处理使F1雌虫的产卵数显著减少了20.72%。与对照组(CK)相比,LD30 处理明显降低了净生殖率(R0)。此外,LD10 和 LD30 处理组的平均世代时间(T)都明显延长。与对照组相比,LD10 和 LD30 组中 SgVg 和 SgVgR 基因的表达量明显降低,这可能是导致雌性繁殖力降低的关键因素。这些结果表明,亚致死剂量的 EMB 能明显降低瓜氏鳖的成虫寿命和繁殖力。这些研究结果对于利用瓜氏天牛和 EMB 制定林业害虫的 IPM 战略非常实用。
{"title":"Sublethal effects of emamectin benzoate on biological characteristics and the expression of vitellogenin (SgVg) and its receptor in Sclerodermus guani","authors":"","doi":"10.1016/j.cropro.2024.106971","DOIUrl":"10.1016/j.cropro.2024.106971","url":null,"abstract":"<div><div>Emamectin benzoate (EMB) is a commonly used insecticide for controlling agricultural and forest pests. To facilitate its rational use in integrated pest management (IPM), the sublethal effects of EMB on the development and reproduction of <em>Scleroderma guani</em> were evaluated. Sublethal doses of EMB (LD<sub>10</sub> and LD<sub>30</sub>) significantly reduced the longevity and fecundity of F<sub>0</sub> females. In the F<sub>1</sub> generation, sublethal doses significantly reduced larval development duration and male longevity but significantly extended female longevity. Additionally, the LD<sub>30</sub> treatment significantly decreased the number of laid eggs in F<sub>1</sub> females by 20.72% compared with the control group. The net reproductive rate (<em>R</em><sub>0</sub>) was significantly declined by the LD<sub>30</sub> treatment compared with the control group (CK). Furthermore, the mean generation time (<em>T</em>) was significantly extended in both LD<sub>10</sub> and LD<sub>30</sub> treatment groups. Compared to the control group, the expression of <em>SgVg</em> and <em>SgVgR</em> genes in the LD<sub>10</sub> and LD<sub>30</sub> groups was significantly lower, which may be a crucial factor for the reduced fecundity of the females. These results indicate that sublethal doses of EMB significantly reduced adult longevity and fecundity of <em>S. guani</em>. The findings are practical for developing IPM strategies for forestry pests using <em>S</em>. <em>guani</em> and EMB.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Garlic as a companion plant for suppressing Myzus persicae infestation in Brassica rapa 将大蒜作为抑制柿蝇蛆侵袭甘蓝型油菜的伴生植物
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-04 DOI: 10.1016/j.cropro.2024.106970
Companion planting, the practice of growing different plant species together, can be a sustainable pest management strategy. However, the specific role of garlic as a companion plant for Brassica in aphid suppression, particularly against Myzus persicae (Sulzer) (Hemiptera: Aphididae), is not well understood. This study investigated the potential of planting garlic (Allium sativum L., Asparagales: Amaryllidaceae) with Brassica rapa L. (Brassicales: Brassicaceae) to reduce M. persicae infestations and explores its impact on the biocontrol agent Harmonia axyridis Pallas (Coleoptera: Coccinellidae). We hypothesized that the combination of A. sativum and B. rapa would synergistically reduce aphid infestations compared to Brassica monocultures. To test this, M. persicae performance was evaluated on Brassica plants under three conditions: a single Brassica plant (B), two Brassica plants (BB), and a Brassica plant with garlic (BG). Parameters such as aphid survival, fecundity, developmental time, and population increase were measured. Additionally, Y-olfactometer bioassays assessed the behavioral responses of M. persicae and H. axyridis. The results showed that the BG combination significantly reduced aphid survival, fecundity, and population growth while delaying developmental time compared to B and BB. M. persicae preferred volatiles from B and BB plants, while H. axyridis was more attracted to BG volatiles, indicating garlic's potential to enhance biocontrol agent recruitment. This study highlights the potential of garlic as a companion plant to improve Brassica crop protection against M. persicae and enhance the effectiveness of biocontrol agents.
伴生种植是将不同植物物种种植在一起的做法,是一种可持续的害虫管理策略。然而,大蒜作为芸苔属植物的伴生植物在抑制蚜虫,特别是抑制蚜蝇(Myzus persicae (Sulzer))(半翅目:蚜科)方面的具体作用还不十分清楚。本研究调查了大蒜(Allium sativum L.,Asparagales: Amaryllidaceae)与芸苔属植物 Brassica rapa L.(Brassicales: Brassicaceae)搭配种植以减少柿蚜虫害的潜力,并探讨了其对生物控制剂 Harmonia axyridis Pallas(鞘翅目:蚜科)的影响。我们假设,与单一栽培芸苔属植物相比,A. sativum 和 B. rapa 的组合将协同减少蚜虫的侵扰。为了验证这一假设,我们在三种条件下评估了 M. persicae 在芸苔属植物上的表现:单一芸苔属植物 (B)、两种芸苔属植物 (BB) 和一种芸苔属植物与大蒜 (BG) 的组合。对蚜虫的存活率、繁殖力、发育时间和种群数量增加等参数进行了测量。此外,Y-olfactometer 生物测定评估了 M. persicae 和 H. axyridis 的行为反应。结果表明,与 B 和 BB 相比,BG 组合能显著降低蚜虫的存活率、繁殖率和种群增长,同时延缓发育时间。M.persicae更喜欢B和BB植物的挥发物,而H. axyridis则更喜欢BG的挥发物,这表明大蒜具有增强生物控制剂招募的潜力。这项研究强调了大蒜作为一种伴生植物的潜力,它可以改善芸苔属作物对M. persicae的保护,并提高生物防治剂的效果。
{"title":"Garlic as a companion plant for suppressing Myzus persicae infestation in Brassica rapa","authors":"","doi":"10.1016/j.cropro.2024.106970","DOIUrl":"10.1016/j.cropro.2024.106970","url":null,"abstract":"<div><div>Companion planting, the practice of growing different plant species together, can be a sustainable pest management strategy. However, the specific role of garlic as a companion plant for <em>Brassica</em> in aphid suppression, particularly against <em>Myzus persicae</em> (Sulzer) (Hemiptera: Aphididae), is not well understood. This study investigated the potential of planting garlic (<em>Allium sativum</em> L., Asparagales: Amaryllidaceae) with <em>Brassica rapa</em> L. (Brassicales: Brassicaceae) to reduce <em>M. persicae</em> infestations and explores its impact on the biocontrol agent <em>Harmonia axyridis</em> Pallas (Coleoptera: Coccinellidae). We hypothesized that the combination of <em>A. sativum</em> and <em>B. rapa</em> would synergistically reduce aphid infestations compared to Brassica monocultures. To test this, <em>M. persicae</em> performance was evaluated on Brassica plants under three conditions: a single <em>Brassica</em> plant (B), two <em>Brassica</em> plants (BB), and a <em>Brassica</em> plant with garlic (BG). Parameters such as aphid survival, fecundity, developmental time, and population increase were measured. Additionally, Y-olfactometer bioassays assessed the behavioral responses of <em>M. persicae</em> and <em>H. axyridis</em>. The results showed that the BG combination significantly reduced aphid survival, fecundity, and population growth while delaying developmental time compared to B and BB. <em>M. persicae</em> preferred volatiles from B and BB plants, while <em>H. axyridis</em> was more attracted to BG volatiles, indicating garlic's potential to enhance biocontrol agent recruitment. This study highlights the potential of garlic as a companion plant to improve <em>Brassica</em> crop protection against <em>M. persicae</em> and enhance the effectiveness of biocontrol agents.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cover crop and herbicides can control purple nutsedge (Cyperus rotundus L.) and increase crop yields in conservation agriculture-based crop rotations 在以保护性农业为基础的轮作中,覆盖作物和除草剂可控制紫花地丁(Cyperus rotundus L.)并提高作物产量
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-04 DOI: 10.1016/j.cropro.2024.106974
<div><div>Adoption of conservation agriculture (CA) practices can lead to the dominance of perennial weeds including purple nutsedge (<em>Cyperus rotundus</em> L.) in different agroecologies over time. Without effective management of this weed, successful adoption of CA in smallholder farming systems is rather unlikely. Therefore, we aimed to develop an integrated strategy for managing <em>C. rotundus</em> in CA-based crop rotations. This study encompassed a two-year (during 2020–21 and 2021–22) field experimentation at Kanpur, India with a split-plot design with three replications. The treatments included purple nutsedge management practices in main plot and crop rotations in sub-plot under CA platform (no-tilled with added crop residues). Six management options were adopted in the summer season (during April to June) as main plots that were super-imposed with two crop rotations such as pigeonpea (<em>Cajanus cajan</em> (L.) Huth) – wheat (<em>Triticum aestivum</em> L.) and pearlmillet (<em>Pennisetum glaucum</em> (L.) R.Br) – chickpea (<em>Cicer arietinum</em> L.) as sub-plot. Growing of cover crop Sesbania (<em>Sesbania aculeata</em> (Willd.) Pers.) during the summer season for 45 days followed by knockdown with 2,4-D 500 g a.i. Ha<sup>−1</sup> (cultural management; Sesbania+2,4-D) and/or summer irrigation followed by application of halosulfuron-methyl 70 g a.i. Ha<sup>−1</sup> at 20 days after irrigation (chemical management; halosulfuron-methyl) reduced the <em>C</em>. <em>rotundus</em> density by 37–42% and 23–64% over years, respectively, than conventional practice (ploughing during summer followed by irrigation and glyphosate 1.5 kg a.e. Ha<sup>−1</sup> application). Besides, these practices could decrease 25–27% total weed density (p < 0.05) than conventional practice (mean of years) in summer season. Importantly, these management practices had a carry-over effect on reduction in density of <em>C</em>. <em>rotundus</em> and total weeds of rainy and winter season crops. For instance, Sesbania +2,4-D and halosulfuron-methyl decreased 35% and 15% density of <em>C</em>. <em>rotundus,</em> respectively, during rainy season than conventional practice. Irrespective of crop rotations, Sesbania +2,4-D and halosulfuron-methyl decreased 30–40% density of <em>C. rotundus</em> (mean of years) in winter season than conventional practice (p < 0.05). A significant reduction in dry weight of <em>C. rotundus</em> was recorded under Sesbania +2,4-D and halosulfuron-methyl in summer season by 13–23%, rainy season by 23–29%, and winter season by 55–72% than conventional practice. Pearlmillet-chickpea rotation had a 10–35% lower infestation of <em>C. rotundus</em> than pigeonpea-wheat (p < 0.05). Management practices such as Sesbania +2,4-D and halosulfuron-methyl had higher system productivity by 15–19% in 2020–21 (p < 0.05), 5–7% in 2021–22 (p > 0.05), and 10–12% in average (p > 0.05) than conventional practice. Summer mungbean cultivation
在不同的农业生态中,采用保护性耕作(CA)方法会导致包括紫花地丁在内的多年生杂草逐渐占据主导地位。如果不对这种杂草进行有效管理,在小农耕作制度中成功采用保护性农业的可能性就相当小。因此,我们的目标是制定一项综合战略,在以 CA 为基础的作物轮作中管理香附子(C. rotundus)。本研究在印度坎普尔进行了为期两年(2020-21 年和 2021-22 年)的田间试验,采用三份重复的分块设计。在 CA 平台(免耕并添加作物秸秆)下,主小区的处理包括紫坚果穗苷管理方法,子小区的处理包括作物轮作。在夏季(4 月至 6 月)采用了六种管理方法,作为主小区,并与鸽子豆(Cajanus cajan (L.) Huth)-小麦(Triticum aestivum L.)和珍珠米(Pennisetum glaucum (L.) R.Br)-鹰嘴豆(Cicer arietinum L.)等两种作物轮作作为副小区叠加。在夏季种植覆盖作物 Sesbania(Sesbania aculeata (Willd.) Pers.)45 天,然后用 2,4-D 500 g a.i.Ha-1(栽培管理;Sesbania+2,4-D)和/或夏季灌溉,然后在灌溉后 20 天施用氟嘧磺隆 70 g a.i.Ha-1(栽培管理;Sesbania+2,4-D)和/或夏季灌溉。与传统方法(夏季犁地,然后灌溉并施用草甘膦 1.5 kg a.e.Ha-1)相比,灌溉后 20 天施用氟嘧磺隆(化学管理;氟嘧磺隆)可使腐霉利密度逐年降低 37-42% 和 23-64%。此外,在夏季,这些方法比传统方法(多年平均值)可降低 25-27% 的杂草总密度(p < 0.05)。重要的是,这些管理方法对降低雨季和冬季作物的轮叶黑麦草密度和杂草总密度具有持续影响。例如,与传统方法相比,谷硫磷 +2,4-D 和甲基吡嘧磺隆在雨季可分别减少 35% 和 15% 的腐竹密度。无论采用哪种轮作方式,石硫合剂+2,4-D 和氟嘧磺隆都能使轮纹草在冬季的密度(年平均值)比常规方法降低 30-40% (p < 0.05)。与传统方法相比,在芝麻+2,4-D 和氟嘧磺隆的作用下,轮纹草的干重在夏季明显减少了 13-23%,在雨季减少了 23-29%,在冬季减少了 55-72%。珍珠米-鹰嘴豆轮作比鸽子豆-小麦轮作的腐霉菌侵扰率低 10-35%(p < 0.05)。与常规方法相比,芝麻+2,4-D 和氟磺隆等管理方法在 2020-21 年的系统生产力提高了 15-19% (p < 0.05),在 2021-22 年提高了 5-7% (p > 0.05),平均提高了 10-12% (p > 0.05)。在 CA 系统中,雨季种植夏绿豆比种植芝麻+2,4-D 和甲基吡嘧磺隆分别增加了 45.2% 和 10.1% 的腐竹密度(p <0.05)。此外,雨季鸽子豆作物下这种杂草的密度比珍珠米作物下高 41.4%(多年平均值)。因此,在种植系统(珍珠米-鹰嘴豆和鸽子豆-小麦)中种植覆盖作物芝麻,然后用 2,4-D 击倒(芝麻 +2,4-D)和/或在夏季通过氟嘧磺隆进行除草剂管理,以及随后推荐的杂草管理方法,都能比传统方法减少轮纹甘蓝和所有杂草的侵扰。
{"title":"Cover crop and herbicides can control purple nutsedge (Cyperus rotundus L.) and increase crop yields in conservation agriculture-based crop rotations","authors":"","doi":"10.1016/j.cropro.2024.106974","DOIUrl":"10.1016/j.cropro.2024.106974","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Adoption of conservation agriculture (CA) practices can lead to the dominance of perennial weeds including purple nutsedge (&lt;em&gt;Cyperus rotundus&lt;/em&gt; L.) in different agroecologies over time. Without effective management of this weed, successful adoption of CA in smallholder farming systems is rather unlikely. Therefore, we aimed to develop an integrated strategy for managing &lt;em&gt;C. rotundus&lt;/em&gt; in CA-based crop rotations. This study encompassed a two-year (during 2020–21 and 2021–22) field experimentation at Kanpur, India with a split-plot design with three replications. The treatments included purple nutsedge management practices in main plot and crop rotations in sub-plot under CA platform (no-tilled with added crop residues). Six management options were adopted in the summer season (during April to June) as main plots that were super-imposed with two crop rotations such as pigeonpea (&lt;em&gt;Cajanus cajan&lt;/em&gt; (L.) Huth) – wheat (&lt;em&gt;Triticum aestivum&lt;/em&gt; L.) and pearlmillet (&lt;em&gt;Pennisetum glaucum&lt;/em&gt; (L.) R.Br) – chickpea (&lt;em&gt;Cicer arietinum&lt;/em&gt; L.) as sub-plot. Growing of cover crop Sesbania (&lt;em&gt;Sesbania aculeata&lt;/em&gt; (Willd.) Pers.) during the summer season for 45 days followed by knockdown with 2,4-D 500 g a.i. Ha&lt;sup&gt;−1&lt;/sup&gt; (cultural management; Sesbania+2,4-D) and/or summer irrigation followed by application of halosulfuron-methyl 70 g a.i. Ha&lt;sup&gt;−1&lt;/sup&gt; at 20 days after irrigation (chemical management; halosulfuron-methyl) reduced the &lt;em&gt;C&lt;/em&gt;. &lt;em&gt;rotundus&lt;/em&gt; density by 37–42% and 23–64% over years, respectively, than conventional practice (ploughing during summer followed by irrigation and glyphosate 1.5 kg a.e. Ha&lt;sup&gt;−1&lt;/sup&gt; application). Besides, these practices could decrease 25–27% total weed density (p &lt; 0.05) than conventional practice (mean of years) in summer season. Importantly, these management practices had a carry-over effect on reduction in density of &lt;em&gt;C&lt;/em&gt;. &lt;em&gt;rotundus&lt;/em&gt; and total weeds of rainy and winter season crops. For instance, Sesbania +2,4-D and halosulfuron-methyl decreased 35% and 15% density of &lt;em&gt;C&lt;/em&gt;. &lt;em&gt;rotundus,&lt;/em&gt; respectively, during rainy season than conventional practice. Irrespective of crop rotations, Sesbania +2,4-D and halosulfuron-methyl decreased 30–40% density of &lt;em&gt;C. rotundus&lt;/em&gt; (mean of years) in winter season than conventional practice (p &lt; 0.05). A significant reduction in dry weight of &lt;em&gt;C. rotundus&lt;/em&gt; was recorded under Sesbania +2,4-D and halosulfuron-methyl in summer season by 13–23%, rainy season by 23–29%, and winter season by 55–72% than conventional practice. Pearlmillet-chickpea rotation had a 10–35% lower infestation of &lt;em&gt;C. rotundus&lt;/em&gt; than pigeonpea-wheat (p &lt; 0.05). Management practices such as Sesbania +2,4-D and halosulfuron-methyl had higher system productivity by 15–19% in 2020–21 (p &lt; 0.05), 5–7% in 2021–22 (p &gt; 0.05), and 10–12% in average (p &gt; 0.05) than conventional practice. Summer mungbean cultivation","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro and Silico Exploration of the Insecticidal Properties of Lavandula multifida L. Essential Oil and its Binary Combinations with Cyantraniliprole and Emamectin benzoate on Spodoptera frugiperda (Lepidoptera: Noctuidae) 多裂叶薰衣草精油及其与氰戊菊酯和苯甲酸阿维菌素二元组合对鞘翅目蚜虫(鳞翅目:夜蛾科)的体外和硅学杀虫特性探索
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-04 DOI: 10.1016/j.cropro.2024.106969
Control methods have faced challenges in effectively managing the spread and damage caused by the fall armyworm (FAW), Spodoptera frugiperda, across various crops worldwide, highlighting the need for more sustainable and integrated approaches. This study explored the insecticidal effect of the essential oil (EO) of lavender (LV) (Lavandula multifida L.), cyantraniliprole (CY), and emamectin benzoate (EM) insecticides on FAW larvae. Toxicity, biological, and biochemical assays were employed to elucidate the impact of the two insecticides individually or combined with LV EO. Additionally, in silico molecular docking investigations were performed for eucalyptol (the major component of LV EO) and for CY against the acetylcholine esterase (AChE) enzyme (PDB ID: 6xyu) and cytochrome P450 (PDB ID: 2q6n). EM was the most toxic compound to FAW larvae with an LC50 of 0.0073 mg L−1, followed by CY and LV EO. The chi-square (χ2) test confirmed the synergistic effect of the LV EO/CY combination and the antagonistic effect LV EO/EM combination on FAW larvae. Biological development studies revealed that both LV EO/CY and LV EO/EM combinations impacted the larval and pupal durations. The biochemical assays indicated that the P450-specific activity was activated by LV EO (at LC25), CY (at LC25 & LC50), and their binary combination (at LC25:LC25). In addition, the LV EO/CY combination significantly activated the AChE and GST-specific activity, and the LV EO/CY combination and CY (at LC50) significantly activated α-esterase- specific activity. Molecular docking investigations revealed energy scores (S) of −6.7729 and −7.7644 kcal/mol for eucalyptol and CY, compared to −7.0993 kcal/mol for PDB ID: 6xyu ligand. The current results can serve as baseline data for utilizing the LV EO/CY combination in the integrated pest management (IPM) of FAW.
在全球范围内,有效控制秋梢虫(FAW)(Spodoptera frugiperda)在各种作物上的传播和危害的控制方法面临着挑战,这凸显了对更具可持续性的综合方法的需求。本研究探讨了薰衣草(Lavandula multifida L.)精油(EO)、氰戊菊酯(CY)和苯甲酸阿维菌素(EM)杀虫剂对FAW幼虫的杀虫效果。通过毒性、生物和生化试验,阐明了这两种杀虫剂单独使用或与 LV EO 合用的影响。此外,还对桉叶油醇(LV EO 的主要成分)和 CY 与乙酰胆碱酯酶(AChE)(PDB ID:6xyu)和细胞色素 P450(PDB ID:2q6n)的分子对接进行了硅学研究。EM 是对一窝蜂幼虫毒性最大的化合物,半数致死浓度为 0.0073 毫克/升,其次是 CY 和 LV EO。秩方(χ2)检验证实了 LV EO/CY 组合的协同效应和 LV EO/EM 组合对草翅虫幼虫的拮抗作用。生物发育研究表明,LV EO/CY 和 LV EO/EM 组合都会影响幼虫和蛹的持续时间。生化试验表明,LV EO(LC25)、CY(LC25 & LC50)以及它们的二元组合(LC25:LC25)激活了 P450 特异性活性。此外,LV EO/CY 组合能显著激活 AChE 和 GST 的特异性活性,LV EO/CY 组合和 CY(LC50 时)能显著激活 α-酯酶的特异性活性。分子对接研究显示,桉叶油醇和 CY 的能量分数(S)分别为 -6.7729 和 -7.7644 kcal/mol,而 PDB ID: 6xyu 配体的能量分数(S)为 -7.0993 kcal/mol。目前的研究结果可作为在一窝蜂虫害综合防治(IPM)中使用 LV EO/CY 组合的基准数据。
{"title":"In vitro and Silico Exploration of the Insecticidal Properties of Lavandula multifida L. Essential Oil and its Binary Combinations with Cyantraniliprole and Emamectin benzoate on Spodoptera frugiperda (Lepidoptera: Noctuidae)","authors":"","doi":"10.1016/j.cropro.2024.106969","DOIUrl":"10.1016/j.cropro.2024.106969","url":null,"abstract":"<div><div>Control methods have faced challenges in effectively managing the spread and damage caused by the fall armyworm (FAW), <em>Spodoptera frugiperda,</em> across various crops worldwide, highlighting the need for more sustainable and integrated approaches. This study explored the insecticidal effect of the essential oil (EO) of lavender (LV) (<em>Lavandula multifida</em> L.), cyantraniliprole (CY), and emamectin benzoate (EM) insecticides on FAW larvae. Toxicity, biological, and biochemical assays were employed to elucidate the impact of the two insecticides individually or combined with LV EO. Additionally, in silico molecular docking investigations were performed for eucalyptol (the major component of LV EO) and for CY against the acetylcholine esterase (AChE) enzyme (PDB ID: <span><span>6xyu</span><svg><path></path></svg></span>) and cytochrome P450 (PDB ID: <span><span>2q6n</span><svg><path></path></svg></span>). EM was the most toxic compound to FAW larvae with an LC<sub>50</sub> of 0.0073 mg L<sup>−1</sup>, followed by CY and LV EO. The chi-square (χ<sup>2</sup>) test confirmed the synergistic effect of the LV EO/CY combination and the antagonistic effect LV EO/EM combination on FAW larvae. Biological development studies revealed that both LV EO/CY and LV EO/EM combinations impacted the larval and pupal durations. The biochemical assays indicated that the P450-specific activity was activated by LV EO (at LC<sub>25</sub>), CY (at LC<sub>25</sub> &amp; LC<sub>50</sub>), and their binary combination (at LC<sub>25</sub>:LC<sub>25</sub>). In addition, the LV EO/CY combination significantly activated the AChE and GST-specific activity, and the LV EO/CY combination and CY (at LC<sub>50</sub>) significantly activated α-esterase- specific activity. Molecular docking investigations revealed energy scores (S) of −6.7729 and −7.7644 kcal/mol for eucalyptol and CY, compared to −7.0993 kcal/mol for PDB ID: <span><span>6xyu</span><svg><path></path></svg></span> ligand. The current results can serve as baseline data for utilizing the LV EO/CY combination in the integrated pest management (IPM) of FAW.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Automatic visual recognition, detection and classification of weeds in cotton fields based on machine vision 基于机器视觉的棉田杂草自动视觉识别、检测和分类
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-02 DOI: 10.1016/j.cropro.2024.106966
Crops and weeds are involved in a continuous competition for equal resources, which may result in a potential decrease in crop yields by up to 31% and an increase in the costs of agricultural inputs by up to 22% of cultivation. Weeds further impact crop production, and their detection is crucial for effective crop management. In this research, we targeted common weeds of cotton field, specifically i) Digitaria sanguinalis (L.) Scop, ii) Amaranthus retroflexus L., iii) Acalypha australis, L., iv) Cephalanoplos segetum, and v) Chenopodium album L. Additionally, image processing techniques such as grayscale conversion, binarization, and Gaussian and morphological filters were also utilized. These methods are based on machine vision and facilitate rapid and straightforward weed detection by segmenting, scrutinizing, and comparing input images. The plant height and area were obtained during cotton planting within 32 days and fitted to develop the growth law concerning planting days for achieving the function of distinguishing cotton from weeds. We conducted recognition experiments by dividing images into four quadrants and categorizing weeds as either inter-row or intra-row. Meanwhile, the inter-row planting information was used to identify weeds, and the leaf pixel area and circularity were used as the identification methods for intra-row weeds, which reduced the algorithm's running time and improved real-time performance. The experimental results indicated that the inter-row weed recognition rate was 89.4%, with an average processing time of 102ms. Whereas in the case of intra-row weeds, the recognition rate was measured at 84.6%, and the overall recognition rate for cotton was 85.0%, with a mean time consumption of 437ms. Furthermore, the present research underscores recent advancements such as machine vision and high-resolution imaging, which have significantly improved the accuracy of automated weed identification in cotton fields while acknowledging ongoing challenges and outlining future opportunities. By Integrating state-of-the-art technology with sustainable agricultural practices, implementing an intelligent system offers a viable approach toward efficient and environmentally friendly weed management in modern agriculture.
农作物和杂草不断争夺同等资源,这可能导致农作物减产达 31%,农业投入成本增加达 22%。杂草会进一步影响作物产量,发现杂草对有效管理作物至关重要。在这项研究中,我们以棉田常见杂草为目标,特别是 i) Digitaria sanguinalis (L.) Scop、ii) Amaranthus retroflexus L.、iii) Acalypha australis, L.、iv) Cephalanoplos segetum 和 v) Chenopodium album L.。此外,我们还利用了灰度转换、二值化、高斯滤波器和形态滤波器等图像处理技术。这些方法以机器视觉为基础,通过分割、仔细检查和比较输入图像,可快速、直接地检测杂草。我们获取了棉花种植 32 天内的株高和面积,并对其进行了拟合,以制定有关种植天数的生长规律,从而实现区分棉花和杂草的功能。我们将图像分为四个象限,并将杂草分为行间杂草和行内杂草,进行了识别实验。同时,利用行间种植信息识别杂草,利用叶片像素面积和圆度作为行内杂草的识别方法,减少了算法的运行时间,提高了实时性。实验结果表明,行间杂草识别率为 89.4%,平均处理时间为 102ms。行内杂草的识别率为 84.6%,棉花的总体识别率为 85.0%,平均处理时间为 437ms。此外,本研究还强调了机器视觉和高分辨率成像等最新进展,这些技术显著提高了棉田杂草自动识别的准确性,同时也承认了当前面临的挑战,并概述了未来的机遇。通过将最先进的技术与可持续农业实践相结合,实施智能系统为现代农业中高效、环保的杂草管理提供了一种可行的方法。
{"title":"Automatic visual recognition, detection and classification of weeds in cotton fields based on machine vision","authors":"","doi":"10.1016/j.cropro.2024.106966","DOIUrl":"10.1016/j.cropro.2024.106966","url":null,"abstract":"<div><div>Crops and weeds are involved in a continuous competition for equal resources, which may result in a potential decrease in crop yields by up to 31% and an increase in the costs of agricultural inputs by up to 22% of cultivation. Weeds further impact crop production, and their detection is crucial for effective crop management. In this research, we targeted common weeds of cotton field, specifically i) <em>Digitaria sanguinalis</em> (L.) Scop, ii) <em>Amaranthus retroflexus</em> L., iii) <em>Acalypha australis</em>, L., iv) <em>Cephalanoplos segetum</em>, and v) <em>Chenopodium album</em> L. Additionally, image processing techniques such as grayscale conversion, binarization, and Gaussian and morphological filters were also utilized. These methods are based on machine vision and facilitate rapid and straightforward weed detection by segmenting, scrutinizing, and comparing input images. The plant height and area were obtained during cotton planting within 32 days and fitted to develop the growth law concerning planting days for achieving the function of distinguishing cotton from weeds. We conducted recognition experiments by dividing images into four quadrants and categorizing weeds as either inter-row or intra-row. Meanwhile, the inter-row planting information was used to identify weeds, and the leaf pixel area and circularity were used as the identification methods for intra-row weeds, which reduced the algorithm's running time and improved real-time performance. The experimental results indicated that the inter-row weed recognition rate was 89.4%, with an average processing time of 102ms. Whereas in the case of intra-row weeds, the recognition rate was measured at 84.6%, and the overall recognition rate for cotton was 85.0%, with a mean time consumption of 437ms. Furthermore, the present research underscores recent advancements such as machine vision and high-resolution imaging, which have significantly improved the accuracy of automated weed identification in cotton fields while acknowledging ongoing challenges and outlining future opportunities. By Integrating state-of-the-art technology with sustainable agricultural practices, implementing an intelligent system offers a viable approach toward efficient and environmentally friendly weed management in modern agriculture.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Soft soap and linalool as potential management tools for Philaenus spumarius (Hemiptera: Aphrophoridae), vector of Xylella fastidiosa 软肥皂和芳樟醇作为潜在的管理工具,可用于防治 Xylella fastidiosa 病媒 Philaenus spumarius(半翅目:蚜蝇科)。
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-01 DOI: 10.1016/j.cropro.2024.106968
Philaenus spumarius (Hemiptera: Aphrophoridae) is one of the most polyphagous xylem-feeding pests. It recently acquired bad fame when it was identified as the main vector of the bacterium Xylella fastidiosa, the causal agent of the Olive Quick Decline Syndrome that, within ten years, has affected and led to the death of more than 21 million olive trees in the Southern Italian region Apulia alone. Despite the agricultural practices and synthetic insecticides available, as not sufficiently effective, more strategies to target all the developmental instars of the pest and eco-friendly formulations, especially in organic orchards, are urgently needed. Therefore, the main goals of this work were to test the toxicity of a solution of potassium salts of fatty acids (soft soap) on P. spumarius juveniles and assess the attractiveness exerted by linalool on adults in a planar olfactometer. According to our results, the average neanids/nymphs’ mortality was 82.2 ± 10.4% 24h after spraying the soft soap on them at the labelled dose. Regarding the adults, linalool was significantly attractive to both sexes in a concentrations range between 0.01 and 0.12 μL linalool L−1 air. Our purpose is to potentially suggest a dual, integrated control approach against P. spumarius, with the soft soap as a biopesticide to reduce the immature stages of the pest and attractive, linalool-activated traps to numerically cull the population of the meadow spittlebug as part of a broader management system.
Philaenus spumarius(半翅目:蚜蝇科)是最多食木质部的害虫之一。最近,这种害虫被确认为 Xylella fastidiosa 细菌的主要传播媒介,从而声名狼藉。Xylella fastidiosa 是橄榄树快速衰退综合症的病原体,仅在意大利南部的阿普利亚地区,十年内就有超过 2100 万棵橄榄树受到影响并死亡。尽管现有的农业措施和合成杀虫剂不够有效,但迫切需要更多针对害虫所有发育阶段的策略和生态友好型配方,尤其是在有机果园中。因此,这项工作的主要目标是测试脂肪酸钾盐溶液(软肥皂)对蚜虫幼虫的毒性,并在平面嗅觉仪中评估芳樟醇对成虫的吸引力。结果表明,按标示剂量喷洒软肥皂 24 小时后,稚虫/幼虫的平均死亡率为 82.2 ± 10.4%。至于成虫,在 0.01 至 0.12 μL 芳樟醇 L-1 的空气浓度范围内,芳樟醇对雌雄成虫都有明显的吸引力。我们的目的是建议一种双重的综合防治方法,即用软肥皂作为生物杀虫剂来减少害虫的未成熟阶段,同时用具有吸引力的芳樟醇活化诱捕器从数量上消灭草地唾液蝽种群,以此作为更广泛的管理系统的一部分来防治草地唾液蝽。
{"title":"Soft soap and linalool as potential management tools for Philaenus spumarius (Hemiptera: Aphrophoridae), vector of Xylella fastidiosa","authors":"","doi":"10.1016/j.cropro.2024.106968","DOIUrl":"10.1016/j.cropro.2024.106968","url":null,"abstract":"<div><div><em>Philaenus spumarius</em> (Hemiptera: Aphrophoridae) is one of the most polyphagous xylem-feeding pests. It recently acquired bad fame when it was identified as the main vector of the bacterium <em>Xylella fastidiosa</em>, the causal agent of the Olive Quick Decline Syndrome that, within ten years, has affected and led to the death of more than 21 million olive trees in the Southern Italian region Apulia alone. Despite the agricultural practices and synthetic insecticides available, as not sufficiently effective, more strategies to target all the developmental instars of the pest and eco-friendly formulations, especially in organic orchards, are urgently needed. Therefore, the main goals of this work were to test the toxicity of a solution of potassium salts of fatty acids (soft soap) on <em>P. spumarius</em> juveniles and assess the attractiveness exerted by linalool on adults in a planar olfactometer. According to our results, the average neanids/nymphs’ mortality was 82.2 ± 10.4% 24h after spraying the soft soap on them at the labelled dose. Regarding the adults, linalool was significantly attractive to both sexes in a concentrations range between 0.01 and 0.12 μL linalool L<sup>−1</sup> air. Our purpose is to potentially suggest a dual, integrated control approach against <em>P. spumarius</em>, with the soft soap as a biopesticide to reduce the immature stages of the pest and attractive, linalool-activated traps to numerically cull the population of the meadow spittlebug as part of a broader management system.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In-vitro and field evaluation of foliar fungicides for the management of Alternaria leaf blight in Radish (Raphanus sativus L.) 用于防治萝卜(Raphanus sativus L.)Alternaria 叶枯病的叶面杀菌剂的体外和田间评估
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-09-30 DOI: 10.1016/j.cropro.2024.106967
Radish, a member of the Brassicaceae family, is a widely cultivated edible root vegetable. Despite its popularity, radish cultivation faces significant challenges due to various pests and diseases, majorly Alternaria leaf blight (ALB) caused by Alternaria brassicicola. To overcome this challenge, an evaluation of eight fungicides consisting of three contact (Chlorothalonil, Propineb, Copper oxychloride), four systemic (Azoxystrobin, Hexaconazole, Propiconazole, Tebuconazole) and a combination (Metalaxyl-M + Mancozeb), was carried out against ALB under in-vitro and field conditions. The efficacy of eight fungicides at varying concentrations (100, 250, 500 and 750 ppm) in inhibiting mycelial growth and conidial germination of A. brassicicola was evaluated. The results showed Tebuconazole to be the most effective fungicide in inhibiting both mycelial growth (100%) and conidial germination (90.70%) at the highest concentration, followed by Propiconazole, Azoxystrobin and Hexaconazole with EC90 of 89.9, 98.8, 105.6, and 101.2 μg mL−1, respectively. Fungicides such as Tebuconazole, Propiconazole and Azoxystrobin had higher conidial germination inhibition, with lower EC50 of 177.2, 198.2 and 200.7 μg mL−1, respectively. To confirm these findings, field trials were conducted over two growing seasons. The foliar spraying of Tebuconazole at 1 ml/L significantly outperformed other treatments, resulting in the lowest PDI (7.67%) and highest radish root yield (13.62 tons/ha). Propiconazole and Azoxystrobin treatments also showed promising results, while untreated controls exhibited the lowest yields of 8.83 tons/ha. Based on the study and information generated, Tebuconazole at 1 ml/L could be recommended and incorporated as a preventive or curative measure in radish ALB management programs.
萝卜是十字花科植物,是一种广泛种植的食用根菜。尽管萝卜很受欢迎,但由于受到各种病虫害的影响,主要是由黄铜交替丝核菌引起的交替丝核菌叶枯病(ALB),萝卜种植面临着巨大的挑战。为了克服这一挑战,研究人员对八种杀菌剂进行了评估,包括三种接触型杀菌剂(百菌清、丙森锌、氧氯化铜)、四种内吸型杀菌剂(唑螨酯、己唑醇、丙环唑、戊唑醇)和一种复配杀菌剂(Metalaxyl-M + Mancozeb)。评估了不同浓度(100、250、500 和 750 ppm)的八种杀菌剂在抑制铜绿菌菌丝生长和分生孢子萌发方面的功效。结果表明,在最高浓度下,戊唑醇是抑制菌丝生长(100%)和分生孢子萌发(90.70%)最有效的杀菌剂,其次是丙环唑、唑螨酯和己唑醇,EC90 分别为 89.9、98.8、105.6 和 101.2 μg mL-1。戊唑醇、丙环唑和唑菌酰胺等杀菌剂对分生孢子发芽的抑制率较高,EC50 分别为 177.2、198.2 和 200.7 μg mL-1。为了证实这些发现,在两个生长季节进行了田间试验。叶面喷施 1 毫升/升的戊唑醇明显优于其他处理,使 PDI 最低(7.67%),萝卜根产量最高(13.62 吨/公顷)。丙环唑和 Azoxystrobin 处理也显示出良好的效果,而未处理的对照组产量最低,为 8.83 吨/公顷。根据这项研究和所获得的信息,可以推荐使用 1 毫升/升的戊唑醇作为萝卜 ALB 管理计划中的预防或治疗措施。
{"title":"In-vitro and field evaluation of foliar fungicides for the management of Alternaria leaf blight in Radish (Raphanus sativus L.)","authors":"","doi":"10.1016/j.cropro.2024.106967","DOIUrl":"10.1016/j.cropro.2024.106967","url":null,"abstract":"<div><div>Radish, a member of the Brassicaceae family, is a widely cultivated edible root vegetable. Despite its popularity, radish cultivation faces significant challenges due to various pests and diseases, majorly Alternaria leaf blight (ALB) caused by <em>Alternaria brassicicola</em>. To overcome this challenge, an evaluation of eight fungicides consisting of three contact (Chlorothalonil, Propineb, Copper oxychloride), four systemic (Azoxystrobin, Hexaconazole, Propiconazole, Tebuconazole) and a combination (Metalaxyl-M + Mancozeb), was carried out against ALB under <em>in-vitro</em> and field conditions. The efficacy of eight fungicides at varying concentrations (100, 250, 500 and 750 ppm) in inhibiting mycelial growth and conidial germination of <em>A. brassicicola</em> was evaluated. The results showed Tebuconazole to be the most effective fungicide in inhibiting both mycelial growth (100%) and conidial germination (90.70%) at the highest concentration, followed by Propiconazole, Azoxystrobin and Hexaconazole with EC<sub>90</sub> of 89.9, 98.8, 105.6, and 101.2 μg mL<sup>−1</sup>, respectively. Fungicides such as Tebuconazole, Propiconazole and Azoxystrobin had higher conidial germination inhibition, with lower EC<sub>50</sub> of 177.2, 198.2 and 200.7 μg mL<sup>−1</sup>, respectively. To confirm these findings, field trials were conducted over two growing seasons. The foliar spraying of Tebuconazole at 1 ml/L significantly outperformed other treatments, resulting in the lowest PDI (7.67%) and highest radish root yield (13.62 tons/ha). Propiconazole and Azoxystrobin treatments also showed promising results, while untreated controls exhibited the lowest yields of 8.83 tons/ha. Based on the study and information generated, Tebuconazole at 1 ml/L could be recommended and incorporated as a preventive or curative measure in radish ALB management programs.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curvularia soli causes a new leaf spot disease on Pereskia aculeata Curvularia soli 在 Pereskia aculeata 上引起一种新的叶斑病
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-09-26 DOI: 10.1016/j.cropro.2024.106965
Pereskia aculeata (Barbados gooseberry) is a vegetable member of the Cactaceae family. Pereskia aculeata leaves are highly valued for culinary use in some Brazilian regions, mainly in Minas Gerais. In March 2023, severe leaf spot disease affecting P. aculeata plants was observed in Viçosa, Minas Gerais, Brazil. Notably, we found that Curvularia sp. was associated with disease symptoms. However, the fungus Curvularia has not been reported to cause any diseases in P. aculeata. Given the absence of any previous records documenting this fungus as a pathogen for this host, this study aimed to identify Curvularia species associated with leaf spots on P. aculeata and elucidate the etiology of the disease. The fungus was identified as Curvularia soli based on its morphological characteristics and molecular features using glyceraldeyde-3-phosphate dehydrogenase (GAPDH) and translation elongation factor 1-α (TEF1-α) for phylogenetic analyses. Healthy P. aculeata plants were inoculated with a representative isolate of C. soli and the same symptoms as observed in the field were reproduced. All steps were performed to fulfill Koch's postulates and confirm the etiology of the disease.
Pereskia aculeata(巴巴多斯醋栗)是仙人掌科植物。在巴西的一些地区,主要是米纳斯吉拉斯州,Pereskia aculeata 的叶子具有很高的烹饪价值。2023 年 3 月,在巴西米纳斯吉拉斯州的维索萨发现了严重的叶斑病,影响到 P. aculeata 植物。值得注意的是,我们发现 Curvularia sp. 与疾病症状有关。但是,还没有报道过 Curvularia 真菌会导致 P. aculeata 发生任何病害。鉴于以前没有任何记录表明这种真菌是这种寄主的病原体,本研究旨在确定与 P. aculeata 叶斑病相关的 Curvularia 物种,并阐明这种疾病的病因。根据真菌的形态特征和分子特征,利用甘油三酯脱氢酶(GAPDH)和翻译伸长因子 1-α (TEF1-α)进行系统发育分析,确定该真菌为 Curvularia soli。用具有代表性的 C. soli 分离物接种健康的 P. aculeata 植物,再现田间观察到的相同症状。所有步骤均符合科赫假说,并确认了病原学。
{"title":"Curvularia soli causes a new leaf spot disease on Pereskia aculeata","authors":"","doi":"10.1016/j.cropro.2024.106965","DOIUrl":"10.1016/j.cropro.2024.106965","url":null,"abstract":"<div><div><em>Pereskia aculeata</em> (Barbados gooseberry) is a vegetable member of the Cactaceae family. <em>Pereskia aculeata</em> leaves are highly valued for culinary use in some Brazilian regions, mainly in Minas Gerais. In March 2023, severe leaf spot disease affecting <em>P. aculeata</em> plants was observed in Viçosa, Minas Gerais, Brazil. Notably, we found that <em>Curvularia</em> sp. was associated with disease symptoms. However, the fungus <em>Curvularia</em> has not been reported to cause any diseases in <em>P. aculeata</em>. Given the absence of any previous records documenting this fungus as a pathogen for this host, this study aimed to identify <em>Curvularia</em> species associated with leaf spots on <em>P. aculeata</em> and elucidate the etiology of the disease. The fungus was identified as <em>Curvularia soli</em> based on its morphological characteristics and molecular features using glyceraldeyde-3-phosphate dehydrogenase (<em>GAPDH</em>) and translation elongation factor 1-α (<em>TEF1-α)</em> for phylogenetic analyses. Healthy <em>P. aculeata</em> plants were inoculated with a representative isolate of <em>C</em>. <em>soli</em> and the same symptoms as observed in the field were reproduced. All steps were performed to fulfill Koch's postulates and confirm the etiology of the disease.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multigenerational hormetic effects of different insecticides on Spodoptera frugiperda (J.E. smith) (Lepidoptera: Noctuidae) 不同杀虫剂对 Spodoptera frugiperda (J.E. smith) (鳞翅目:夜蛾科)的多代激素效应
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-09-24 DOI: 10.1016/j.cropro.2024.106962
To protect crops from pest insects, farmers continuously use insecticides, which eventually degrade into residue due to abiotic or biotic factors. These residual effects of pesticides may cause low lethal and/or sublethal impacts on the exposed pest insect populations, leading to the induction of pest resurgence through hormesis and ultimately the development of resistance. Hormesis is a beneficial bi-phasic effect generally characterized by low-dose reproductive stimulation and high-dose inhibition. In the present study, we investigated the impact of low lethal and sublethal concentrations (LC10, LC20, and LC30) of spinetoram, chlorantraniliprole, and thiodicarb on the F0 generation as well as their multigenerational hormesis effects on the F1 and F2 generations of Fall Armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). The study focused on reproductive parameters, fecundity, fertility, and life index parameters such as net reproductive rate (R0), intrinsic rate of increase (rm), finite rate of increase (λ), mean length of generation (Tc), and gross reproductive rate (GRR). Fecundity of S. frugiperda increased by 4.82%, 4.63% and 4.58%, while eggs fertility improved by 7.77%, 6.78% and 6.52% at LC10 of thiodicarb, chlorantraniliprole and spinetoram, respectively, from the F1 to F2 generation. Further, interaction analysis (Concentration × Insecticidal treatments) revealed that important vital parameters like pre-oviposition period (POP), oviposition period (OP), post-oviposition period (PtOP), R0, rm, λ, Tc and GRR of F0 were negatively impacted by LC30 of all the tested insecticides and LC20 of only spinetoram. However, LC10 of any of the tested insecticides did not affect these parameters when compared with control. Furthermore, interactive analysis in multigenerational exposure experiments (Concentration × Insecticidal treatments × Generation(s)) showed hermetic effects of LC10 of all tested insecticides which is more positive in F2 as compared to F1 generation. These unwanted positive hermetic effects of low lethal concentrations (LC10) of tested insecticides in offspring of exposed parental population of S. frugiperda indicated its resistance and/or resurgence. Furthernore, present study emphasizes the necessity for additional research to elucidate the underlying mechanisms responsible for these effects.
为了保护农作物免受害虫侵害,农民不断使用杀虫剂,但由于非生物或生物因素,杀虫剂最终会降解成残留物。杀虫剂的这些残留效应可能会对暴露在有害昆虫种群中的害虫造成低致死率和/或亚致死率影响,从而通过激素作用诱导害虫复发,并最终产生抗药性。激素作用是一种有益的双相效应,一般具有低剂量生殖刺激和高剂量抑制的特点。在本研究中,我们调查了低致死浓度和亚致死浓度(LC10、LC20 和 LC30)的辛硫磷、氯虫苯甲酰胺和硫代双脒对秋陆虫(Spodoptera frugiperda (J.E. Smith))(鳞翅目:夜蛾科)F0 代的影响,以及它们对 F1 代和 F2 代的多代激效作用。研究的重点是繁殖参数、繁殖力、生育力和生命指数参数,如净繁殖率(R0)、内在增长率(rm)、有限增长率(λ)、平均世代长度(Tc)和总繁殖率(GRR)。从 F1 代到 F2 代,在硫丹、氯虫苯甲酰胺和辛硫磷的 LC10 浓度下,S. frugiperda 的繁殖力分别提高了 4.82%、4.63% 和 4.58%,卵的受精率分别提高了 7.77%、6.78% 和 6.52%。此外,交互作用分析(浓度×杀虫处理)显示,F0 的重要生命参数,如卵着前期(POP)、卵着期(OP)、卵着后期(PtOP)、R0、rm、λ、Tc 和 GRR 均受到所有测试杀虫剂的 LC30 和仅刺五加的 LC20 的负面影响。然而,与对照组相比,任何一种测试杀虫剂的 LC10 都不会影响这些参数。此外,多代接触实验(浓度×杀虫处理×世代)中的交互分析表明,与 F1 代相比,所有测试杀虫剂的 LC10 对 F2 代的气密性影响更大。受试杀虫剂的低致死浓度(LC10)对暴露亲本种群的后代产生的这些不需要的正封闭效应表明其具有抗性和/或复性。此外,本研究强调有必要开展更多研究,以阐明造成这些影响的潜在机制。
{"title":"Multigenerational hormetic effects of different insecticides on Spodoptera frugiperda (J.E. smith) (Lepidoptera: Noctuidae)","authors":"","doi":"10.1016/j.cropro.2024.106962","DOIUrl":"10.1016/j.cropro.2024.106962","url":null,"abstract":"<div><div>To protect crops from pest insects, farmers continuously use insecticides, which eventually degrade into residue due to abiotic or biotic factors. These residual effects of pesticides may cause low lethal and/or sublethal impacts on the exposed pest insect populations, leading to the induction of pest resurgence through hormesis and ultimately the development of resistance. Hormesis is a beneficial bi-phasic effect generally characterized by low-dose reproductive stimulation and high-dose inhibition. In the present study, we investigated the impact of low lethal and sublethal concentrations (LC<sub>10</sub>, LC<sub>20</sub>, and LC<sub>30</sub>) of spinetoram, chlorantraniliprole, and thiodicarb on the F<sub>0</sub> generation as well as their multigenerational hormesis effects on the F<sub>1</sub> and F<sub>2</sub> generations of Fall Armyworm (FAW), <em>Spodoptera frugiperda</em> (J.E. Smith) (Lepidoptera: Noctuidae). The study focused on reproductive parameters, fecundity, fertility, and life index parameters such as net reproductive rate (<em>R</em><sub><em>0</em></sub>), intrinsic rate of increase (<em>r</em><sub><em>m</em></sub>), finite rate of increase (<em>λ</em>), mean length of generation (<em>T</em><sub><em>c</em></sub>), and gross reproductive rate (<em>GRR</em>). Fecundity of <em>S. frugiperda</em> increased by 4.82%, 4.63% and 4.58%, while eggs fertility improved by 7.77%, 6.78% and 6.52% at LC<sub>10</sub> of thiodicarb, chlorantraniliprole and spinetoram, respectively, from the F<sub>1</sub> to F<sub>2</sub> generation. Further, interaction analysis (Concentration × Insecticidal treatments) revealed that important vital parameters like pre-oviposition period (POP), oviposition period (OP), post-oviposition period (PtOP), <em>R</em><sub><em>0,</em></sub> <em>r</em><sub><em>m,</em></sub> <em>λ, T</em><sub><em>c</em></sub> and <em>GRR</em> of F<sub>0</sub> were negatively impacted by LC<sub>30</sub> of all the tested insecticides and LC<sub>20</sub> of only spinetoram. However, LC<sub>10</sub> of any of the tested insecticides did not affect these parameters when compared with control. Furthermore, interactive analysis in multigenerational exposure experiments (Concentration × Insecticidal treatments × Generation(s)) showed hermetic effects of LC<sub>10</sub> of all tested insecticides which is more positive in F<sub>2</sub> as compared to F<sub>1</sub> generation. These unwanted positive hermetic effects of low lethal concentrations (LC<sub>10</sub>) of tested insecticides in offspring of exposed parental population of <em>S. frugiperda</em> indicated its resistance and/or resurgence. Furthernore, present study emphasizes the necessity for additional research to elucidate the underlying mechanisms responsible for these effects.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Crop Protection
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1