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Effect of integration of chemical fertilizers with organic manures on growth, yield and nutrient uptake in hybrid maize (Zea mays L.) ​ ​ 有机肥配施对杂交玉米生长、产量及养分吸收的影响
Pub Date : 2022-11-15 DOI: 10.31830/2454-1761.2022.cr-834
G. Baradhan, M. Saranya, S. S. Kumar, R. Immanuel, G. S. Rao
Maize (Zea mays L.) becoming very popular cereal crop in India because of high production potential of hybrid varieties and it requires heavy doses of fertilizers. But the excessive and continuous use of chemical fertilizers has damaged the soil health, at the same time, the sole use of organic manure cannot compensate the produce obtained by inorganic application. Hence, it is important to reduce the usage of chemical fertilizers and supplementing the same through organic manures. Therefore, field experiment was conducted in Experimental Farm of Annamalai University during 2020 to evaluate the effect of different levels of inorganic fertilizers with different sources of organic manures on the growth, yield and nutrient uptake in hybrid maize. The experiment was laid out in randomized block design with nine treatments viz., 100% recommended dose of fertilizer (RDF), 100% RDF + Seaweed granules @ 20 kg/ha, 100% RDF + Pressmud granules @ 125 kg/ha, 100% RDF + Poultry manure @ 2.5 t/ha, 100% RDF + Urban waste compost @ 10 t/ha, 75% RDF + Seaweed granules @ 20 kg/ha, 75% RDF + Pressmud granules @ 125 kg/ha, 75% RDF + Poultry manure @ 2.5 t/ha, 75% RDF + Urban waste compost @ 10 t/ha and replicated thrice. The results revealed that plant height, leaf area index, dry matter production, grain and stover yield and benefit : cost ratio were favourably influenced by the application of 100% RDF+Poultry manure @ 2.5 t/ha. In nutrient status, uptake of nitrogen and phosphorus was found to be significantly higher with application of 100% RDF+Poultry manure @ 2.5 t/ha, whereas potassium uptake was higher under application of 100% RDF+Pressmud granules @ 125 kg/ha. Thus, for achieving higher productivity, integrated application of 100% RDF with poultry manure @ 2.5 t/ha may be preferred to the hybrid maize cultivating farmers.
玉米(Zea mays L.)在印度成为非常受欢迎的谷类作物,因为杂交品种具有很高的生产潜力,而且需要大量的肥料。但是化肥的过量和持续使用破坏了土壤的健康,同时,单一使用有机肥不能补偿无机施用所获得的产量。因此,重要的是减少化学肥料的使用,并用有机肥料补充化学肥料。为此,本研究于2020年在安纳玛莱大学实验农场进行田间试验,评价不同水平无机肥配不同来源有机肥对杂交玉米生长、产量和养分吸收的影响。实验是在与九治疗即随机区组设计,推荐剂量的肥料(RDF) 100%, 100% RDF +海藻颗粒@ 20公斤/公顷,100% RDF + Pressmud颗粒@ 125公斤/公顷,100% RDF +家禽粪便@ 2.5吨/公顷,100% RDF +城市垃圾堆肥@ 10吨/公顷,75% RDF +海藻颗粒@ 20公斤/公顷,75% RDF + Pressmud颗粒@ 125公斤/公顷,75% RDF +家禽粪便@ 2.5吨/公顷,75% RDF +城市垃圾堆肥@ 10吨/公顷,重复三次。结果表明,施用100% RDF+ 2.5 t/ha的禽粪对水稻株高、叶面积指数、干物质产量、籽粒和秸秆产量及效益成本比均有显著影响。在养分状态下,施用100% RDF+家禽粪(2.5 t/ha)显著提高了氮和磷的吸收量,而施用100% RDF+压力颗粒(125 kg/ha)的钾吸收量更高。因此,为了获得更高的生产力,100% RDF与2.5 t/ha禽粪的综合施用可能更适合杂交玉米种植农民。
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引用次数: 1
Efficacy of new generation herbicidal combination for weed management in direct seeded rice (Oryza sativa L.) 新一代除草组合对直播水稻杂草治理效果的研究
Pub Date : 2022-11-15 DOI: 10.31830/2454-1761.2022.cr-874
R. Suganya, S. S. Kumar, G. Baradhan
Weeds are the main yield limiting factor that can potentially reduce crop yields and is one of the main constraints in the larger scale implementation of direct seeded rice. Therefore, the present experiment was conducted at Periyanarkunam during Navarai 2021 to study the efficacy of new generation herbicidal combination for weed management in direct seeded rice. The experiment was laid out in split plot design replicated thrice with main plot treatments comprised of three direct seeded rice establishment methods viz., dry seeded, drum seeded and manual wet seeded rice. The sub-treatments were nine weed management practices with combination of herbicides with hand weeding viz., Pre-emergence application of bensulfuron methyl 0.6%+pretilachlor 6% GR @ 10 kg/ha on 8 DAS (Days after sowing), early post- emergence application of triafamone 20%+ethoxysulfuron 10% WG @ 200 g/ha on 12 DAS, post-emergence application of metsulfuron methyl 10%+chlorimuron ethyl 10% @ 20 g/ha on 25 DAS, twice hand weeding on 25 and 45 DAS and un-weeded check. Among the main treatments, drum seeded rice recorded lower weed population and dry matter production, higher weed control efficiency, weed control index, crop resistance index and herbicide efficiency index and higher grain yield which was at par with manual wet seeded rice. Regarding the weed management practices evaluated pre-emergence application of bensulfuron methyl 0.6%+pretilachlor 6% GR @ 10 kg/ha on 8 DAS fb (followed by) post-emergence application of metsulfuron methyl 10%+chlorimuron ethyl 10% @ 20 g/ha on 25 DAS fb hand weeding on 45 DAS (S8) registered lower weed population, dry matter and higher yield compared to unweeded check. Hence, drum seeded rice coupled with pre-emergence application on 8 DAS fb post-emergence application on 25 DAS fb hand weeding on 45 DAS (M2S8) is an economically feasible method for effective control of weeds.
杂草是可能降低作物产量的主要产量限制因素,也是大规模实施直接播种水稻的主要制约因素之一。因此,本试验在Navarai 2021期间在Periyanarkunam进行,研究新一代除草剂组合对直播水稻杂草管理的效果。试验采用3次重复的分割小区设计,主要小区处理包括3种直接播种法,即干播、鼓播和手工湿播水稻。分处理为9种除草剂与手除草相结合的杂草管理措施,即:苗期前施用甲基苯磺隆0.6%+苯甲草胺6% GR @ 10 kg/ha, 8日(播种后),苗期早期施用曲曲霉酮20%+乙氧基磺隆10% WG @ 200 g/ha, 12日(播种后),苗期后施用甲基甲磺隆10%+乙基氯脲10% @ 20 g/ha, 25日(播种后),两次手除草25日和45日(播种后)和未除草检查。在主要处理中,套筒播种水稻的杂草数量和干物质产量较低,杂草防治效率、杂草防治指数、作物抗性指数和除草剂效率指数较高,籽粒产量与人工湿播水稻相当。在杂草管理方面,评估了苗期前在8株DAS fb上施用甲基苯磺隆0.6%+甲酰草胺6% GR @ 10 kg/ha(其次是苗期后在25株DAS fb上施用甲基甲磺隆10%+乙基氯脲10% @ 20 g/ha)与未除草相比,在45株DAS (S8)上进行手动除草,发现杂草数量、干物质和产量均较低。因此,稻筒播种配合苗期前施用8 DAS fb,苗期后施用25 DAS fb,手除草45 DAS (M2S8)是一种经济可行的有效防治杂草的方法。
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引用次数: 0
Influence of integrated nutrient management on the physiology of Chinnar ‘a local land race’ of rice (Oryza sativa L.) 综合营养管理对中国稻(Oryza sativa L.)生理的影响
Pub Date : 2022-11-15 DOI: 10.31830/2454-1761.2022.cr-875
Challa Venkata Nitesh Reddy, S. A. H. Hussainy, R. Singh, V. Paulpandi
Rice is the staple cereal crop of India as well as Tamil Nadu. Consumers tend to prefer local cultivars and landraces of rice which have region-specific importance. Chinnar is such a local landrace of rice that is preferred by the consumers of Madurai because of its nutritional quality. Like other cultivars, Chinnar responds to added nutrients for enhancement of growth. Integrated nutrient management (INM) is one of the suitable options in crop and soil health management where inorganic and organic sources of nutrients are used. This study was carried out during 2022 at Agricultural College and Research Institute, Madurai to evaluate the influence of INM on the growth of the Chinnar landrace of rice. Recommended dose of fertilizer (RDF) with respect to inorganic nutrient sources viz., fertilizers (Urea, SSP and MOP) @ 50% and the remaining with organic manures through poultry manure, goat manure, farm yard manure, enriched farm yard manure and vermicompost were applied as per nitrogen equivalent basis. Results revealed higher leaf area index, chlorophyll content of Chinnar at various stages of growth phase. Thus, from the study, it is concluded that INM can be preferred to obtain higher growth attributes of Chinnar rice in Madurai conditions of Tamil Nadu.
水稻是印度和泰米尔纳德邦的主要谷物作物。消费者倾向于选择对地区具有特殊重要性的当地品种和地方品种的大米。Chinnar是一种当地的地方大米品种,由于其营养质量而受到马杜赖消费者的青睐。像其他品种一样,金盏花对添加的营养物质有反应,以促进生长。综合养分管理(INM)是作物和土壤健康管理的合适选择之一,其中使用无机和有机养分来源。本研究于2022年在马杜赖农业学院和研究所进行,旨在评估INM对中国地方水稻生长的影响。无机营养源(即肥料(尿素、SSP和MOP))的推荐用量(RDF)为50%,其余为禽粪、羊粪、农场粪肥、强化农场粪肥和蚯蚓堆肥等有机粪肥,按氮当量施用。结果表明:在不同的生长阶段,金盏花的叶面积指数和叶绿素含量较高。因此,本研究认为,在泰米尔纳德邦的马杜赖条件下,INM可以优先获得中国水稻更高的生长属性。
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引用次数: 0
Response of maize (Zea mays L.) to different nitrogen doses under the mild tropical plain agroclimatic zone of North-East India 印度东北部温和热带平原农业气候带下玉米对不同施氮量的响应
Pub Date : 2022-11-15 DOI: 10.31830/2454-1761.2022.cr-878
Mayanglambam Sanjit Singh, Chowlani Manpoong
Formulating optimum quantity of nitrogen fertilization is highly necessary to attain higher yields in maize. As per soil fertility, the right dosage has to be formulated for each region with consciousness on the soil and environmental health. A field experiment was conducted during 2018-19 under mild tropical plain agroclimatic conditions of Arunachal Pradesh, India to investigate the effect of different nitrogen doses on growth and yield of maize. The experiment was laid out under randomized block design with five replicated plots for each treatment. The nitrogen through urea was supplied with different doses of 0, 100, 120, 150 and 180 kg/ha. The crop was evaluated for plant height, number of leaves, leaf length, number of kernels per cob, number of rows per cob, and total grain yield. The results revealed that the variables such as plant height, number of leaves, leaf length, number of kernels per cob, number of rows per cob and total grain yield were significantly (P less than 0.05) affected by the different levels of nitrogen doses. Number of leaves, leaf length and crop yield were highest in 180 kg/ha (nitrogen dose), while lowest in control which significantly increased with higher nitrogen doses. This result showed that nitrogen with higher doses was more effective than low doses for maize crop. Nitrogen fertilization at 180 kg/ha exerted a significantly higher and positive effect on maize growth and can be recommended for the region.
制定最佳施氮量对玉米产量的提高是十分必要的。根据土壤肥力,必须为每个具有土壤和环境健康意识的地区制定合适的剂量。本试验于2018- 2019年在印度**温和热带平原农业气候条件下,研究不同施氮量对玉米生长和产量的影响。试验采用随机区组设计,每个处理设5个重复区。分别以0、100、120、150和180 kg/ hm2的不同剂量尿素供氮。对作物的株高、叶数、叶长、每芯粒数、每芯行数和总产量进行了评价。结果表明,不同施氮量对水稻株高、叶数、叶长、每芯粒数、每芯行数和籽粒总产量均有显著影响(P < 0.05)。叶片数、叶长和产量在180 kg/ha(施氮量)时最高,而对照最低,且随施氮量的增加而显著增加。结果表明,高剂量氮肥对玉米作物的施用效果优于低剂量氮肥。施氮量为180 kg/ hm2的氮肥对玉米生长有显著的正向促进作用,可在该地区推广。
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引用次数: 0
Yield enhancement in sesame (Sesamum indicum L.) var. TMV 7 through weed management practices  通过杂草管理措施提高芝麻(Sesamum indicum L.) var. TMV 7产量
Pub Date : 2022-11-15 DOI: 10.31830/2454-1761.2022.cr-880
P. Elakkiyapriya, M. Meyyappan, P. Sudhakar, A. Angayarkanni
A field experiment was conducted during summer season of 2021 with a view to find out a suitable weed management practice for sesamum by excluding commonly used herbicides and including botanical herbicides along with hand weeding. The experiment was conducted in randomized block design with 10 treatments and was replicated thrice. The treatments included one pre-emergence herbicide metribuzin at two levels, one early post- emergence herbicide imazethapyr and one post-emergence herbicide quizalofop ethyl and were applied individually and in sequence along with HW. Two plant products Ageratum conizoides and Ocimum sanctum were tried each at two levels along with HW. These treatments were compared with twice HW and unweeded control. Among the treatments, the least weed density (9.92, 9.88), weed DMP (4.96, 13.54 g/m2), highest grain yield (684 kg/ha) and stalk yield (2069 kg/ha) were recorded in pre-emergence application of metribuzin @ 100 g a.i./ha on 3 DAS+Early post-emergence application of imazethapyr @ 30 g a.i./ha on 10 DAS+HW on 20 DAS. Further, this treatment gave the highest BCR of 2.46. Treatments involving herbicides fb HW were better than plant products fb HW treatments in terms of weed density and yield.
在2021年夏季进行了田间试验,通过排除常用除草剂,采用植物除草剂和人工除草,找出适合芝麻杂草管理的做法。试验采用随机区组设计,共10个处理,重复3次。苗期前除草剂甲曲霉嗪、苗期后除草剂吡唑乙酯和苗期后除草剂吡曲霉乙酯分别为2个水平,按不同剂量和不同顺序施用。两种植物产品荆刺和荆刺随HW分别在两个水平上进行试验。这些处理与两次高施和未除草对照进行了比较。拔苗期前施用100 g a.i./ha 3个DAS+拔苗期早期施用30 g a.i./ha 10个DAS+HW 20个DAS的处理,杂草密度最低(9.92、9.88),杂草DMP分别为4.96、13.54 g/ha,籽粒产量最高(684 kg/ha),茎秆产量最高(2069 kg/ha)。该处理的BCR最高,为2.46。在杂草密度和产量方面,除草剂和除草剂处理均优于植物产品和除草剂处理。
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引用次数: 0
Performance of different sources of nitrogen under different establishment methods of rice (Oryza sativa  不同氮素源在不同建立方法下的表现
Pub Date : 2022-11-15 DOI: 10.31830/2454-1761.2022.cr-873
Sai Kumar Midde, M. Perumal, G. Murugan, R. Sudhagar
Currently, conventional fertilizers are used in large quantities to deliver nutrients to crops in India. It may result in soil health decline and fertilizer waste. Different techniques, such as nano fertilizers, are required to address this issue. Therefore, a field experiment was laid out in a farmer's field in the Chidambaram block during the kuruvai season of 2021-22 to determine the "Performance of different sources of nitrogen under different establishment methods of rice." The experiment was carried out in a split-plot design, which included three methods of crop establishment viz., mechanical transplanting, the system of rice intensification (SRI) and conventional transplanting in the main plot, and five nitrogen management methods viz., 100% RDN through urea, 100% RDN through vermicompost, 50% RDN through urea+50% RDN through nano urea, 50% RDN through vermicompost + 50% RDN through nano urea, and 100% RDN through nano urea in sub-plot replicated thrice. The experiment findings demonstrated that among the establishment techniques and nitrogen management practices tested, the system of rice intensification and application of 50% inorganic nitrogen in combination with 50% nano nitrogen was the most productive in terms of plant height (90.29 ,105.39 cm), number of tillers/m2 (337.39, 403.15), dry matter production (10135, 12331 kg/ha) and crop growth rate (10.35, 12.41 g/m2/day) resulting in higher grain (4794, 5972 kg/ha) and straw yield (7216, 8692 kg/ha). Nano fertilizer application promoted the growth and development activity in rice and demonstrated the potential to improve crop production and plant nutrition.
目前,印度大量使用传统肥料来向农作物输送养分。它可能导致土壤健康下降和肥料浪费。解决这个问题需要不同的技术,比如纳米肥料。因此,在2021-22年库鲁瓦伊季节,在Chidambaram区块的一个农民田间进行了一项田间试验,以确定“不同氮源在不同水稻建立方法下的性能”。试验采用分畦设计,主田采用机械移栽、水稻集约化系统(SRI)和常规移栽3种建种方式,分畦采用100% RDN尿素、100% RDN蚯蚓堆肥、50% RDN尿素+50% RDN纳米尿素、50% RDN蚯蚓堆肥+50% RDN纳米尿素、100% RDN纳米尿素5种氮肥管理方式,重复3次。试验结果表明,在所试验的水稻栽培技术和氮素管理措施中,50%无机氮与50%纳米氮结合的水稻强化施用体系在株高(90.29、105.39 cm)、分蘖数(337.39、403.15)、干物质产量(10135、12331 kg/ha)和作物生长率(10.35、12.41 g/m2/day)方面效果最佳,籽粒产量(4794、5972 kg/ha)和秸秆产量(7216、8692公斤/公顷)。施用纳米肥料促进了水稻的生长发育活动,显示出改善作物产量和植物营养的潜力。
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引用次数: 0
 Physiological characterization for abiotic stress tolerance in rice (Oryza sativa) genotypes  水稻(Oryza sativa)基因型非生物抗逆性的生理特性
Pub Date : 2022-08-17 DOI: 10.31830/2454-1761.2022.808
V. N, S. T, R. V
Abiotic stresses in rice restrict the growth and potential of the crop hampering the production and productivity. Screening for abiotic stress tolerance was conducted for salinity and water stress (1% and 2%) during Kharif 2019 at Regional Agricultural Research Station, Maruteru, Andhra Pradesh, India. The physiological characterization of selected genotypes showed that among all treatments, seed germination, root length, shoot length and seedling vigour index was greater under control followed by 1% mannitol, 2% mannitol and salinity stress. The entries IET 27773, IET 27750 and IET 26861 performed well under moderate water stress (1% mannitol and 2% mannitol induced stress) and hence can be identified as relatively tolerant. Under salinity stress AC 1303 followed by IET 27750, IET 27772 and IET 27773 performed well. The above cultures can be used as physiological donors for respective stresses. The seedling vigour was highest in IET 27773 under all the stress treatments. IET 27750 and IET 27773 showed tolerance to both salinity and water stress. In the field experiment, IET 26961 (6230 kg/ha), IET 27768 (6060 kg/ha) and IET 27773 (6050 kg/ha) recorded the highest grain yield. Among all the genotypes tested, IET 27773 was found to be a good performing genotype as it exhibited tolerance to abiotic stress in lab conditions and recorded higher yields under field conditions.
水稻的非生物胁迫限制了作物的生长和潜力,阻碍了产量和生产力。2019年哈里夫期间,在印度安得拉邦马鲁特鲁区域农业研究站对盐度和水分胁迫(1%和2%)进行了非生物抗逆性筛选。所选基因型的生理特性表明,在所有处理中,1%甘露醇处理、2%甘露醇处理和盐胁迫处理的种子发芽率、根长、茎长和幼苗活力指数均高于对照处理。条目IET 27773、IET 27750和IET 26861在中度水分胁迫(1%甘露醇和2%甘露醇诱导的胁迫)下表现良好,因此可以确定为相对耐受性。在盐度胁迫下,AC 1303表现较好,IET 27750、IET 27772和IET 27773表现较好。上述培养物可作为相应应激的生理供体。各胁迫处理下,IET 27773幼苗活力最高。IET 27750和IET 27773对盐度和水分胁迫均表现出耐受性。在田间试验中,IET 26961 (6230 kg/ha)、IET 27768 (6060 kg/ha)和IET 27773 (6050 kg/ha)的产量最高。在所有测试的基因型中,IET 27773被认为是表现较好的基因型,因为它在实验室条件下表现出对非生物胁迫的耐受性,在田间条件下取得了较高的产量。
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引用次数: 1
Effect of plant growth regulators on growth, yield and quality attributes of watermelon (Citrullus lanatus Thunb.) 植物生长调节剂对西瓜生长、产量和品质性状的影响
Pub Date : 2022-08-12 DOI: 10.31830/2454-1761.2022.801
P. Kumar, M. C. S. Rao, A. Tamang, U. S. Kumar
Plant growth regulators are called as magic chemicals which are new generation agrochemicals. Exogenous application of plant growth regulators in small quantity can alter the sequence of male and female flowers, if applied at 2 or 4 leaf stages, the critical stage at which the suppression or promotion of either sex is possible. Therefore, a field experiment was carried out during Rabi, 2021 at Horticulture Research Farm, M.S. Swaminathan School of Agriculture, Centurion University of Technology and Management, Parlakhemundi, Odisha to study the influence of plant growth regulators on growth, yield and quality attributes of watermelon.Theexperiment was laid out under randomized block design with nine treatments namely 2, 4-D (25 and 50 ppm), NAA (100 and 150 ppm), GA3 (30 and 40 ppm), TIBA (20 and 25 ppm) and control (distilled water)replicated thrice. Two sprays of plant growth regulators were done at 2nd and 4th true leaf stages. The results revealed that, growth characters such as length of the main creeper (2.08 m), number of sub creepers per plant (4.40) and chlorophyll content (61.93 and 48.91 at 30 and 60 DAS) were highest in treatment (GA3 @ 40 ppm). The chlorophyll content at 90 DAS was highest (21.39) in treatment (NAA @ 100 ppm). The highest fruit yield plant-1 (17.51 kg) and fruit yield per hectare (44.17 t) was recorded in treatment (GA3 @ 40 ppm). The quality attributes such as Total Soluble Solid (TSS) (10.66%) and total sugars (15.03 %) were noted highest in treatment (GA3 @ 40 ppm). Among the different plant growth regulators spraying of GA3 @ 40 ppm found effective in enhancing the growth, yield and quality attributes of watermelon.
植物生长调节剂被称为神奇化学品,是新一代农用化学品。少量外源施用植物生长调节剂可以改变雄花和雌花的序列,如果在2或4叶期施用,这是可能抑制或促进任何性别的关键阶段。因此,在2021年拉比期间,在奥里萨邦Parlakhemundi百夫长技术与管理大学M.S. Swaminathan农业学院园艺研究农场进行了田间试验,研究植物生长调节剂对西瓜生长、产量和品质属性的影响。试验采用随机区组设计,设2,4 - d(25和50 ppm)、NAA(100和150 ppm)、GA3(30和40 ppm)、TIBA(20和25 ppm)和对照(蒸馏水)9个处理,重复3次。植物生长调节剂在第2和第4真叶期喷施2次。结果表明,在GA3 @ 40 ppm处理下,植株主匍匐茎长(2.08 m)、副匍匐茎数(4.40 m)和叶绿素含量(61.93和48.91)最高。叶绿素含量在90 DAS时以NAA @ 100 ppm处理最高(21.39)。在GA3 @ 40 ppm处理下,植株-1果实产量最高(17.51 kg),每公顷果实产量最高(44.17 t)。总可溶性固形物(TSS)(10.66%)和总糖(15.03%)等品质属性在处理(GA3 @ 40 ppm)时最高。在不同的植物生长调节剂中,喷施40 ppm的GA3对西瓜的生长、产量和品质有显著的促进作用。
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引用次数: 0
Standardization of an efficient protocol for transformation in Indian cauliflower (Brassica oleracea) var. botrytis  印度花椰菜(芸苔)芽孢变种高效转化方案的标准化
Pub Date : 2022-07-28 DOI: 10.31830/2454-1761.2022.cr-11088
Shalini Rawat, P. Kalia, N. Goel
Cauliflower is an important Cole crop grown all over the world for its nutritional benefits. But it is encountered by a serious problem caused by the insect- pest attack. Plutella xylostella is one such insect which causes huge losses. An efficient and simple method for genetic transformation and regeneration of Indian cauliflower, Brassica oleracea var. botrytis has to be developed. Hence, optimization of the plant transformation is a pre- requisite for successful transformation process. Therefore, we worked on this objective at the Indian Agricultural Research Institute, New Delhi, India, and optimized the Agrobacterium-mediated transformation protocol in Indian cauliflower, Pusa Snowball K1 and Pusa Snowball KT25 varieties for developing insect resistance against Plutella xylostella using Bt genes. The Agrobacterium tumefaciens mediated transformation process was utilized for carrying out the transformation and the explant was obtained from the seedlings germinated from the seeds of the respective variety and used for genetic transformation process. The transgene integration and expression were later confirmed by PCR and RT-qPCR, respectively. Moreover, insect bioassay showed that transgenic plants effectively controlled the Diamond Back moth infestation concerning to the control plants indicating the presence of resistance developed against the insect. Hence, this protocol efficiently regenerated transgenic plants that lead to the resistance against biotic stress, specific to the insect Diamond back moth.
菜花因其丰富的营养价值,是一种重要的油菜籽作物。但是却遇到了一个严重的问题,那就是虫害的侵袭。小菜蛾就是造成巨大损失的昆虫之一。一种高效、简便的印度菜花(Brassica oleracea var. botrytis)遗传转化和再生方法有待开发。因此,植物转化的优化是成功转化过程的先决条件。为此,我们在印度新德里的印度农业研究所(Indian Agricultural Research Institute)开展了这一工作,优化了农杆菌介导的印度菜花、Pusa Snowball K1和Pusa Snowball KT25品种转化方案,利用Bt基因培养对小菜蛾的抗性。利用农杆菌介导的转化过程进行转化,从各品种种子萌发的幼苗中获得外植体,进行遗传转化。随后分别用PCR和RT-qPCR验证转基因的整合和表达。昆虫生物测定结果表明,转基因植株对菱形背蛾的侵染有较好的控制作用,表明转基因植株对菱形背蛾产生了抗性。因此,该方案有效地再生转基因植物,导致对生物胁迫的抗性,特别是对昆虫钻石背蛾。
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引用次数: 0
Study on the invasive alien species in ponds of Jalaun district of Uttar Pradesh, India 印度北方邦贾劳恩地区池塘外来入侵物种研究
Pub Date : 2022-07-21 DOI: 10.31830/2454-1761.2022.cr-822
V. Yadav, Sonam Sharma
Invasive alien plant species are widely vulnerable to invasion and recognized as stresses altering the ecosystem properties and competing with native flora of pond. During the investigation a total of 30 invasive alien species belonging to 26 genera under 17 families were recorded. Asteraceae was the most dominant family with 5 species and about 79% of these invasive alien species were introduced from Tropical America. Among invase alien species, major potential threat in the near future is by Argemone maxicana and Parthenium histophorus. It was observed that 18 plant species were being used by local inhabitant for medicinal purposes. In order to resist the spread, a better planning is needed for early detection of control, report infestation of spread of new and naturalized invasive alien species to be monitored.
外来入侵植物是一种易受入侵的物种,被认为是改变池塘生态系统特性和与本地植物区系竞争的压力。调查共发现外来入侵植物30种,隶属于17科26属。菊科为优势科,共有5种,其中79%来自热带美洲。在外来入侵物种中,近期的潜在威胁主要是银顶草和柏顶草。据观察,当地居民将18种植物用作医药用途。为了抵御其蔓延,需要制定更好的计划,及早发现控制,报告外来入侵新物种和归化物种的侵扰传播,并对其进行监测。
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Crop research
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