Developmental cell最新文献

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IF 11.8 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-22 DOI: 10.1016/j.devcel.2025.11.008

Vanessa Weichselberger, Gareth Moore, Sham Tlili, Matthias Merkel, Pierre-François Lenne, Vikas Trivedi

Organoids, 3D in vitro structures derived from embryonic or adult stem cells, offer powerful models for studying tissue patterning, development, morphogenesis, organ physiology, and disease. These systems replicate biological processes, such as cell differentiation, symmetry breaking, and tissue organization, while revealing species-specific developmental variations. Biophysical factors, such as extracellular matrix composition, cell motility, tissue flows, and stiffness, interact with biochemical signals to drive organoid formation, revealing complex multiscale phenomenon during growth, patterning, and homeostasis. Physics-based approaches provide a framework to understand these processes from first principles. In recent years, a growing community of researchers has been exploring what can be termed the “biophysics of organoids.” This review covers a broad range of approaches—mechanical, kinetic, information-based, statistical, and artificial intelligence (AI)-driven—to study organoid development, offering insights into organogenesis, disease modeling, and regenerative medicine.

类器官是来源于胚胎或成体干细胞的3D体外结构，为研究组织模式、发育、形态发生、器官生理学和疾病提供了强大的模型。这些系统复制生物过程，如细胞分化、对称性破坏和组织组织，同时揭示物种特异性发育变异。生物物理因素，如细胞外基质组成、细胞运动、组织流动和硬度，与生化信号相互作用，驱动类器官形成，揭示生长、模式和稳态过程中复杂的多尺度现象。基于物理的方法提供了一个从第一性原理理解这些过程的框架。近年来，越来越多的研究人员一直在探索所谓的“类器官生物物理学”。本综述涵盖了机械、动力学、信息、统计和人工智能（AI）驱动等广泛的方法来研究类器官的发育，为器官发生、疾病建模和再生医学提供了见解。

引用次数: 0

Generating 3D mouse organoids for cortex development and evo-devo. 生成用于皮层发育和进化的三维小鼠类器官。

IF 8.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-15 DOI: 10.1016/j.devcel.2025.11.006

Hanwen Yu, Yan Liu

How do ci-regulatory differences, across mouse subspecies and over evolutionary timescales, shape cell-type specification and maturation during corticogenesis? In this issue of Developmental Cell, Medina-Cano et al. establish a scalable mouse organoid platform that recapitulates developmental dynamics and enables mapping of allele-specific expression, linking cis-regulatory variation with neurodevelopmental mechanisms and disease-associated genetics.

在皮质发生过程中，不同小鼠亚种和进化时间尺度上的ci调节差异如何影响细胞类型规范和成熟？在本期《发育细胞》中，Medina-Cano等人建立了一个可扩展的小鼠类器官平台，该平台概括了发育动力学，并能够绘制等位基因特异性表达图谱，将顺式调控变异与神经发育机制和疾病相关遗传学联系起来。

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Single-cell transcriptomic analysis highlights specific cell types manipulated by Fusarium head blight fungus leading to wheat susceptibility. 单细胞转录组学分析强调了小麦对赤霉病的敏感性。

IF 8.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-15 DOI: 10.1016/j.devcel.2025.09.015

Wan-Qian Wei, Shuang Li, Dong Zhang, Wei-Hua Tang

引用次数: 0

Rapid adaptation of the basement membrane composition to mechanical needs. 基膜组成对机械需求的快速适应。

IF 8.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-15 DOI: 10.1016/j.devcel.2025.09.005

Uwe Töpfer, Anne Holz

The mechanisms by which basement membranes (BMs) adjust their mechanical properties to meet the biophysical needs of organs remain unclear. In this issue of Developmental Cell, Soh et al. establish the Caenorhabditis spermatheca as a model for repeated stretching and relaxation of the BM during the continuous ovulation process and demonstrate high, fibulin-dependent elasticity.

基底膜（basal membrane, BMs）调节其力学性能以满足器官生物物理需求的机制尚不清楚。在本期的《发育细胞》中，Soh等人建立了精膜Caenorhabditis spermatheca作为连续排卵过程中BM反复拉伸和松弛的模型，并显示出高度的纤维蛋白依赖性弹性。

引用次数: 0

A matter of selectivity: ER-phagy suppression at the onset of pancreatic cancer. 选择性问题：胰腺癌发病时er吞噬抑制。

IF 8.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-15 DOI: 10.1016/j.devcel.2025.11.005

Carmine Settembre

KRAS mutations drive pancreatic ductal adenocarcinoma (PDAC). In this issue of Developmental Cell, Salomó Coll et al.¹ reveal that KRAS suppresses endoplasmic reticulum (ER)-phagy in pancreatic acinar cells by inhibiting CCPG1 transcription. Impaired ER-phagy triggers protein aggregation, inflammation, and acinar-to-ductal metaplasia, promoting tumorigenesis. These findings highlight selective autophagy's role in cancer, with possible therapeutic implications.

KRAS突变驱动胰腺导管腺癌（PDAC）。在这一期的Developmental Cell中，Salomó Coll等人1发现KRAS通过抑制CCPG1转录抑制胰腺腺泡细胞的内质网（ER）吞噬。受损的er吞噬触发蛋白质聚集、炎症和腺泡到导管的化生，促进肿瘤发生。这些发现强调了选择性自噬在癌症中的作用，可能具有治疗意义。

引用次数: 0

Primary cilia as architects of the neocortex: Roles in brain development, function, and microcephaly 初级纤毛作为新皮质的建筑师：在大脑发育、功能和小头畸形中的作用

IF 11.8 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-04 DOI: 10.1016/j.devcel.2025.11.002

Oskar Kaaber Thomsen, Jindřiška Leischner Fialová, Canan Doganli, Cristian Herrera-Cid, Kjeld Møllgård, Alexandre Benmerah, Lars Allan Larsen, Søren Tvorup Christensen

The etiology of primary hereditary microcephaly (MCPH), a condition closely linked to neocortex development, remains poorly understood. Initially, MCPH genes were thought to regulate a limited set of cellular processes, but recent studies reveal that many encode multifunctional proteins, often converging on primary cilia, organelles that orchestrate the development of most vertebrate tissues and organs. In this perspective article, we examine the role of primary cilia in brain development and explore how disruptions in MCPH and microcephaly-associated proteins compromise ciliary dynamics and function. We highlight additional cilia-related proteins with potential influence on neurodevelopment. By elucidating the connections between primary cilia and neural development, we aim to provide insights into mechanisms of neuroregeneration. Ultimately, advancing our understanding of primary cilia may help develop therapeutic strategies to restore neuronal function and improve outcomes for individuals with neurodevelopmental disorders.

原发性遗传性小头畸形（MCPH）是一种与新皮质发育密切相关的疾病，其病因尚不清楚。最初，MCPH基因被认为调节有限的细胞过程，但最近的研究表明，许多基因编码多功能蛋白质，通常聚集在初级纤毛上，这是协调大多数脊椎动物组织和器官发育的细胞器。在这篇前瞻性文章中，我们研究了初级纤毛在大脑发育中的作用，并探讨了MCPH和小头症相关蛋白的破坏如何损害纤毛的动力学和功能。我们强调了对神经发育有潜在影响的其他纤毛相关蛋白。通过阐明初级纤毛与神经发育之间的联系，我们旨在为神经再生的机制提供见解。最终，推进我们对初级纤毛的理解可能有助于制定治疗策略，以恢复神经功能并改善神经发育障碍患者的预后。

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引用次数: 0

Dual role of Oct4 and Sox2 in controlling the developmental capacity and timing of tissue morphogenesis in the embryonic lineage Oct4和Sox2在胚胎谱系中控制发育能力和组织形态发生时间的双重作用

IF 11.8 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-02 DOI: 10.1016/j.devcel.2025.11.003

Deepthi Chandramohan, Rongge Yan, Kai Kruse, Heike Brinkmann, Hyun-Woo Jeong, Yanlin Hou, Kenjiro Adachi, Hans R. Schöler, Ivan Bedzhov

The fundamental processes of cell fate specification, differentiation, and morphogenesis must be finely synchronized to enable proper developmental progression, yet the molecular factors coordinating these processes are not well understood. A key driver of embryonic morphogenesis is the establishment of epithelial polarity, which organizes and structures the early cell layers. Here, we investigated factors controlling the epithelialization in epiblast cells and implemented sequential loss-of-function approaches in mouse embryos to define the timing of developmental significance. We found that the expression wave of the core pluripotency factors Oct4 and Sox2 following the 8-cell stage plays a critical role in this process. In this context, one of the key shared functions of these factors is to prevent premature activation of the epithelial program until the completion of the second lineage segregation. Thus, Oct4 and Sox2 simultaneously govern developmental capacity and regulate the developmental timing of tissue morphogenesis of the embryonic lineage.

细胞命运规范、分化和形态发生的基本过程必须精细同步才能实现适当的发育进程，然而协调这些过程的分子因素尚未得到很好的理解。胚胎形态发生的一个关键驱动因素是上皮极性的建立，这是早期细胞层的组织和结构。在这里，我们研究了控制外胚层细胞上皮化的因素，并在小鼠胚胎中实施了顺序功能丧失方法，以确定发育意义的时间。我们发现核心多能因子Oct4和Sox2在8细胞期之后的表达波在这一过程中起着关键作用。在这种情况下，这些因子的一个关键共享功能是防止上皮程序的过早激活，直到完成第二谱系分离。因此，Oct4和Sox2同时调控胚胎谱系组织形态发生的发育能力和发育时间。

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引用次数: 0

Un-LOX-ing tumor immune barriers 消除肿瘤免疫屏障

IF 11.8 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-01 DOI: 10.1016/j.devcel.2025.10.016

Tuyen T. Dang, Srinivas Malladi

In this issue of Developmental Cell, Zhang et al. report the identification of two transcriptionally distinct renal cell carcinoma tumor thrombus subtypes. The aggressive TT1 subtype is characterized by LOX-expressing cancer cells within an immunosuppressive microenvironment dominated by THBS2⁺ fibroblasts and GPNMB⁺ macrophages that exclude CD8⁺ T cells.

在这一期的Developmental Cell上，Zhang等报道了两种转录不同的肾细胞癌肿瘤血栓亚型的鉴定。侵袭性TT1亚型的特征是在免疫抑制微环境中表达lox的癌细胞，该微环境由THBS2+成纤维细胞和GPNMB+巨噬细胞主导，不包括CD8+ T细胞。

引用次数: 0

The symphony of shape: A feedback loop conducting zebrafish heart formation 形状的交响乐：一个引导斑马鱼心脏形成的反馈回路

IF 11.8 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-01 DOI: 10.1016/j.devcel.2025.10.003

Changchang Cao, Zheng Li, Li Wang

Organ structures formed during development dictate their physiological functions. In this issue of Developmental Cell, Andrews et al.¹ demonstrate a cellular feedback loop linking trabeculation and compact layer stretching, which depends on actomyosin remodeling mediated by the Notch signaling pathway and orchestrates ventricular morphogenesis in zebrafish.

发育过程中形成的器官结构决定了它们的生理功能。在本期《发育细胞》（Developmental Cell）杂志上，Andrews等人1展示了一个连接小梁形成和致密层拉伸的细胞反馈回路，该回路依赖于Notch信号通路介导的肌动yosin重塑，并协调斑马鱼的心室形态发生。

引用次数: 0

Combinatorial BMP4 and activin direct the choice between alternate routes to endoderm in a stem cell model of human gastrulation. 在人原肠胚干细胞模型中，组合BMP4和激活素指导通往内胚层的替代途径的选择。

IF 8.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell

Pub Date : 2025-12-01 Epub Date: 2025-09-09 DOI: 10.1016/j.devcel.2025.08.009

Oliver C K Inge, Elias Copin, Jake Cornwall-Scoones, Borzo Gharibi, Irene Rodriguez-Hernandez, Pablo Soro-Barrio, Molly Strom, Probir Chakravarty, James Briscoe, Silvia D M Santos

Lineage specification requires accurate interpretation of multiple signaling cues. However, how combinatorial signaling histories influence fate outcomes remains unclear. We combined single-cell transcriptomics, live-cell imaging, and mathematical modeling to explore how activin and bone morphogenetic protein 4 (BMP4) guide fate specification during human gastrulation. We see that these signals interact both synergistically and antagonistically to drive fate decisions. We find that definitive endoderm arises from lineage convergence: a direct route from pluripotency and an indirect route via a mesoderm progenitor state. Cells pass through temporal windows of signaling competency, and the relative concentration of activin and BMP4 dictates the trajectory choice. The efficiency between routes is underpinned by a dual role of BMP4 in inducing mesoderm genes while promoting pluripotency exit. This work underscores that the combination of signals a cell is exposed to not only directs its final fate but also the developmental route taken, suggesting lineage convergence enhances robustness in fate specification.

谱系规范需要对多个信号线索进行准确的解释。然而，组合信号历史如何影响命运结果仍不清楚。我们结合单细胞转录组学、活细胞成像和数学模型来探索激活素和骨形态发生蛋白4 （BMP4）如何指导人类原肠胚形成过程中的命运规范。我们看到，这些信号相互作用，协同和对抗，以驱动命运的决定。我们发现最终的内胚层起源于谱系趋同：多能性的直接途径和中胚层祖细胞状态的间接途径。细胞通过信号能力的时间窗口，激活素和BMP4的相对浓度决定了轨迹的选择。BMP4在诱导中胚层基因和促进多能性退出的双重作用支持了途径之间的效率。这项工作强调，细胞所暴露的信号组合不仅指导其最终命运，而且还指导其所采取的发育路线，这表明谱系收敛增强了命运规范的稳健性。

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