Pub Date : 2025-10-20DOI: 10.1016/j.devcel.2025.09.014
Keqiang Wu
In this issue of Developmental Cell, Ge et al. 1 reveal that drought-induced calcium signaling in tomato activates the SlCBL11-SlCIPK10 complex, which phosphorylates the chromatin protein SlLHP1b. This modification enhances H3K27me3-mediated repression of stamen SlYUC genes, lowering auxin levels and collapsing the abscission zone gradient to trigger flower drop.
{"title":"When flowers fall: Calcium-driven epigenetic silencing links drought to reproductive loss in tomato.","authors":"Keqiang Wu","doi":"10.1016/j.devcel.2025.09.014","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.09.014","url":null,"abstract":"<p><p>In this issue of Developmental Cell, Ge et al. <sup>1</sup> reveal that drought-induced calcium signaling in tomato activates the SlCBL11-SlCIPK10 complex, which phosphorylates the chromatin protein SlLHP1b. This modification enhances H3K27me3-mediated repression of stamen SlYUC genes, lowering auxin levels and collapsing the abscission zone gradient to trigger flower drop.</p>","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"60 20","pages":"2699-2700"},"PeriodicalIF":8.7,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-15DOI: 10.1016/j.devcel.2025.09.021
Meng-Jia Wang, Yi-Fang Tsay
The nitrate transceptor NRT1.1 detects external nitrate levels to regulate nitrate-induced transcriptional responses, auxin transport, and phosphate starvation responses in plants. A study by Ma et al.1 redefines our understanding of abscisic acid (ABA) perception and reveals NRT1.1 as a signaling hub that integrates the nutrient status with abiotic stresses.
{"title":"NRT1.1 is a versatile coordinator of nutrient and hormone signaling in plants","authors":"Meng-Jia Wang, Yi-Fang Tsay","doi":"10.1016/j.devcel.2025.09.021","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.09.021","url":null,"abstract":"The nitrate transceptor NRT1.1 detects external nitrate levels to regulate nitrate-induced transcriptional responses, auxin transport, and phosphate starvation responses in plants. A study by Ma et al.<span><span><sup>1</sup></span></span> redefines our understanding of abscisic acid (ABA) perception and reveals NRT1.1 as a signaling hub that integrates the nutrient status with abiotic stresses.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"1 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145289268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fertilization of the egg cell by more than one sperm cell results in aneuploidy and genomic dysfunction. To avoid this, flowering plants have intricate polytubey block mechanisms that prevent additional pollen tubes from entering the ovule. In one mechanism for blocking polytubey, the fertilized egg cell secretes the aspartic endopeptidases EGG CELL-SPECIFIC1 (ECS1) and ECS2, which degrade pollen tube attractants. However, the mechanism underlying the egg-cell-specific expression of ECS1 and ECS2 was unknown. Here, we demonstrate that in Arabidopsis thaliana egg cells, SMALL RNA DEGRADING NUCLEASE (SDN) degrades microRNAs (miRNAs) targeting ECS genes, thereby allowing ECS1 and ECS2 to accumulate in egg cells to establish the polytubey block. Like Arabidopsis ecs1 ecs2 mutants, sdn1 sdn2 sdn3 mutants exhibited polytubey, as did plants specifically overexpressing miRNAs targeting ECS genes in egg cells. Therefore, the SDN-miRNA-ECS module allows ECS accumulation in egg cells to block polytubey.
{"title":"Degradation of detrimental microRNAs safeguards the fertilized egg cells to establish an ECS-dependent polytubey block in Arabidopsis","authors":"Kun Shen, Mengxue Qu, Xuemei Zhou, Yingying Guo, Yicheng Zhong, Meng-xiang Sun, Peng Zhao","doi":"10.1016/j.devcel.2025.09.013","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.09.013","url":null,"abstract":"Fertilization of the egg cell by more than one sperm cell results in aneuploidy and genomic dysfunction. To avoid this, flowering plants have intricate polytubey block mechanisms that prevent additional pollen tubes from entering the ovule. In one mechanism for blocking polytubey, the fertilized egg cell secretes the aspartic endopeptidases EGG CELL-SPECIFIC1 (ECS1) and ECS2, which degrade pollen tube attractants. However, the mechanism underlying the egg-cell-specific expression of <em>ECS1</em> and <em>ECS2</em> was unknown. Here, we demonstrate that in <em>Arabidopsis thaliana</em> egg cells, SMALL RNA DEGRADING NUCLEASE (SDN) degrades microRNAs (miRNAs) targeting <em>ECS</em> genes, thereby allowing ECS1 and ECS2 to accumulate in egg cells to establish the polytubey block. Like <em>Arabidopsis ecs1 ecs2</em> mutants, <em>sdn1 sdn2 sdn3</em> mutants exhibited polytubey, as did plants specifically overexpressing miRNAs targeting <em>ECS</em> genes in egg cells. Therefore, the SDN-miRNA-ECS module allows ECS accumulation in egg cells to block polytubey.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"120 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-09DOI: 10.1016/j.devcel.2025.09.011
Aurélie Lardenois, Antonio Suglia, Chad Lewis Moore, Bertrand Evrard, Laurence Noël, Paul Rivaud, Aurore Besson, Maryne Toupin, Joséphine Blévinal, Corentin Dumortier, Simon Léonard, Laurianne Lesné, Isabelle Coiffec, Serge Nef, Vincent Lavoué, Anthony Bretaudeau, Alain Chédotal, Séverine Mazaud-Guittot, Frédéric Chalmel, Antoine Dominique Rolland
Gonad development is an exciting model to study cell fate commitment. A better understanding of sex determination requires the identification of all involved cell types and their dynamic expression programs. Here, we present an atlas of 128,000 single cells from human gonads between 5 and 12 post-conceptional weeks. A focused analysis of somatic cells uncovered a population of bipotential progenitors derived from the coelomic epithelium of both testes and ovaries, which may have the capacity to commit to either a steroidogenic or a supporting fate. Moreover, our analyses suggest that early supporting cells, prior to differentiation into Sertoli or pre-granulosa cells, also give rise to the rete testis/ovarii and that the ovary retains the capacity to feed the supporting cell pool for an extended period of time, directly from the surface epithelium. Finally, the potential involvement of the gonadotropin releasing hormone (GnRH) signaling pathway in regulating testis differentiation was assessed ex vivo.
{"title":"Single-cell exploration of gonadal somatic cell lineage specification during human sex determination","authors":"Aurélie Lardenois, Antonio Suglia, Chad Lewis Moore, Bertrand Evrard, Laurence Noël, Paul Rivaud, Aurore Besson, Maryne Toupin, Joséphine Blévinal, Corentin Dumortier, Simon Léonard, Laurianne Lesné, Isabelle Coiffec, Serge Nef, Vincent Lavoué, Anthony Bretaudeau, Alain Chédotal, Séverine Mazaud-Guittot, Frédéric Chalmel, Antoine Dominique Rolland","doi":"10.1016/j.devcel.2025.09.011","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.09.011","url":null,"abstract":"Gonad development is an exciting model to study cell fate commitment. A better understanding of sex determination requires the identification of all involved cell types and their dynamic expression programs. Here, we present an atlas of 128,000 single cells from human gonads between 5 and 12 post-conceptional weeks. A focused analysis of somatic cells uncovered a population of bipotential progenitors derived from the coelomic epithelium of both testes and ovaries, which may have the capacity to commit to either a steroidogenic or a supporting fate. Moreover, our analyses suggest that early supporting cells, prior to differentiation into Sertoli or pre-granulosa cells, also give rise to the <em>rete testis</em>/<em>ovarii</em> and that the ovary retains the capacity to feed the supporting cell pool for an extended period of time, directly from the surface epithelium. Finally, the potential involvement of the gonadotropin releasing hormone (GnRH) signaling pathway in regulating testis differentiation was assessed <em>ex vivo</em>.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"50 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-09DOI: 10.1016/j.devcel.2025.09.012
Zuoxing Wu, Na Li, Zhengqiong Luo, Zihan Chen, Xuemei He, Fan Shi, Jie Han, Haitao Huang, Baohong Shi, Long Zhang, Yu Li, Junmin Shen, Seoyeon Bok, Jun Sun, Xing Niu, Ke Mo, Pengbin Yin, Lige Leng, Xin Wang, Jie Zhang, Ren Xu
Low bone mass is frequently observed in Alzheimer’s disease (AD), yet the underlying mechanisms remain poorly understood. In this study, we demonstrate that sensory nerves constitute a critical component of the skeletal stem cell (SSC) niche. Deletion of the neurotrophin receptor p75NTR in neurons or sensory-specific cells, but not in osteogenic or sympathetic cells, resulted in reduced sensory innervation, disrupted SSC homeostasis, and significant bone loss. Although a cell-intrinsic role of p75NTR in SSCs cannot be ruled out, further experiments involving sensory denervation or transplantation into hosts with sensory-neuron-specific p75NTR deficiency confirmed impaired SSC osteogenesis. Mechanistically, p75NTR controls the expression of neuronal osteopontin (SPP1), which in turn promotes SSC self-renewal and osteogenic differentiation. Notably, this p75NTR-SPP1 signaling axis was found to be disrupted in AD mouse models, offering a direct mechanistic explanation for AD-associated osteopenia and highlighting the therapeutic potential of targeting neural control of SSCs.
{"title":"The p75 neurotrophin receptor controls the skeletal stem cell niche through sensory innervation","authors":"Zuoxing Wu, Na Li, Zhengqiong Luo, Zihan Chen, Xuemei He, Fan Shi, Jie Han, Haitao Huang, Baohong Shi, Long Zhang, Yu Li, Junmin Shen, Seoyeon Bok, Jun Sun, Xing Niu, Ke Mo, Pengbin Yin, Lige Leng, Xin Wang, Jie Zhang, Ren Xu","doi":"10.1016/j.devcel.2025.09.012","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.09.012","url":null,"abstract":"Low bone mass is frequently observed in Alzheimer’s disease (AD), yet the underlying mechanisms remain poorly understood. In this study, we demonstrate that sensory nerves constitute a critical component of the skeletal stem cell (SSC) niche. Deletion of the neurotrophin receptor p75NTR in neurons or sensory-specific cells, but not in osteogenic or sympathetic cells, resulted in reduced sensory innervation, disrupted SSC homeostasis, and significant bone loss. Although a cell-intrinsic role of p75NTR in SSCs cannot be ruled out, further experiments involving sensory denervation or transplantation into hosts with sensory-neuron-specific p75NTR deficiency confirmed impaired SSC osteogenesis. Mechanistically, p75NTR controls the expression of neuronal osteopontin (SPP1), which in turn promotes SSC self-renewal and osteogenic differentiation. Notably, this p75NTR-SPP1 signaling axis was found to be disrupted in AD mouse models, offering a direct mechanistic explanation for AD-associated osteopenia and highlighting the therapeutic potential of targeting neural control of SSCs.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"9 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-06DOI: 10.1016/j.devcel.2025.08.006
Darya Khantakova, Marco Colonna
In this issue of Developmental Cell, Ma et al. show that embryonically derived VSIG4⁺ macrophages suppress CD8⁺ T cell responses across cancers. They identify IL-11 as a key effector and MEF2C as a transcriptional regulator of VSIG4⁺ macrophages, highlighting new therapeutic avenues for targeting immunosuppressive tumor-associated macrophages to improve immunotherapy outcomes.
{"title":"Dual threat: VSIG4⁺ macrophages use IL-11 and VSIG4 to silence T cells","authors":"Darya Khantakova, Marco Colonna","doi":"10.1016/j.devcel.2025.08.006","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.006","url":null,"abstract":"In this issue of <em>Developmental Cell</em>, Ma et al. show that embryonically derived VSIG4⁺ macrophages suppress CD8⁺ T cell responses across cancers. They identify IL-11 as a key effector and MEF2C as a transcriptional regulator of VSIG4⁺ macrophages, highlighting new therapeutic avenues for targeting immunosuppressive tumor-associated macrophages to improve immunotherapy outcomes.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"158 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-06DOI: 10.1016/j.devcel.2025.07.009
Brigida Novello, Cédric Blanpain
In this issue of Developmental Cell, Journot et al. identify a conserved mechanism promoting the development and lineage segregation of multipotent stem cells across different glandular epithelia. p63, YAP, and Notch control symmetry breaking, cell positioning, and cell-fate decision during development and regeneration, illustrating how spatial cues orchestrate tissue self-organization.
{"title":"Common mechanisms regulate stem cell self-organization and symmetry breaking across various glandular epithelia","authors":"Brigida Novello, Cédric Blanpain","doi":"10.1016/j.devcel.2025.07.009","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.07.009","url":null,"abstract":"In this issue of <em>Developmental Cell</em>, Journot et al. identify a conserved mechanism promoting the development and lineage segregation of multipotent stem cells across different glandular epithelia. p63, YAP, and Notch control symmetry breaking, cell positioning, and cell-fate decision during development and regeneration, illustrating how spatial cues orchestrate tissue self-organization.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"82 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-06DOI: 10.1016/j.devcel.2025.06.011
Wenhui Chen, Yanjie Shen, Guangshuai Jia
Chronic inflammation shapes the tumor microenvironment and influences cancer stem cell behavior. In this issue of Developmental Cell, Gu et al. identify TNFAIP6 as a key responder to tumor necrosis factor alpha (TNF-α) that promotes self-renewal of glioblastoma stem cells and reprograms pro-inflammatory macrophages toward an immunosuppressive phenotype, identifying a therapeutic vulnerability in glioblastoma.
{"title":"How glioblastoma stem cells turn inflammatory cues into tumor growth and immune suppression","authors":"Wenhui Chen, Yanjie Shen, Guangshuai Jia","doi":"10.1016/j.devcel.2025.06.011","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.06.011","url":null,"abstract":"Chronic inflammation shapes the tumor microenvironment and influences cancer stem cell behavior. In this issue of <em>Developmental Cell</em>, Gu et al. identify TNFAIP6 as a key responder to tumor necrosis factor alpha (TNF-α) that promotes self-renewal of glioblastoma stem cells and reprograms pro-inflammatory macrophages toward an immunosuppressive phenotype, identifying a therapeutic vulnerability in glioblastoma.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"52 4 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-06DOI: 10.1016/j.devcel.2025.09.006
Fangxia Wang, Yuu Kimata
Cells exiting quiescence must simultaneously prepare for DNA replication and boost metabolism. Paul et al.1 now show that mitogen-activated mTOR transiently suppresses APC/C-CDH1, unleashing the glycolytic activator PFKFB3 to provide an energetic pulse that jump-starts proliferation before APC/C is reactivated.
{"title":"Kickstart the cell cycle with a sugar boost: mTOR brake on APC/C-CDH1 triggers a glycolytic pulse","authors":"Fangxia Wang, Yuu Kimata","doi":"10.1016/j.devcel.2025.09.006","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.09.006","url":null,"abstract":"Cells exiting quiescence must simultaneously prepare for DNA replication and boost metabolism. Paul et al.<span><span><sup>1</sup></span></span> now show that mitogen-activated mTOR transiently suppresses APC/C-CDH1, unleashing the glycolytic activator PFKFB3 to provide an energetic pulse that jump-starts proliferation before APC/C is reactivated.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"75 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145229364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-02DOI: 10.1016/j.devcel.2025.09.009
Rongfeng Huang, Jiacheng Wang, Mingzeng Chang, Wenxin Tang, Yongqiang Yu, Yi Zhang, Yao Peng, Yanan Wang, Yihan Guo, Ting Lu, Yang Cao, Yewei Zhou, Qinglin Zhang, Yuehong Huang, Angxiao Wu, Luyan Ren, Michelle Gallei, Juan Dong, Haodong Chen, Jun He, Tongda Xu
The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis.
植物激素生长素在调节植物生长发育中的关键作用通常与其定向运输有关，这种定向运输依赖于质膜（PM）上的极化PIN- formed （PIN）生长素出口蛋白。几十年来，生长素被认为促进PIN极化，产生自我调节反馈，介导了植物的大部分发育，但缺乏对这种调节的机制认识。在这里，我们在PM发现了生长素诱导的蛋白复合物，包含生长素共受体跨膜激酶（TMKs）和生长素输出PIN1，作为这种反馈调节的核心机制。生长素通过TMKs促进PIN1磷酸化，调节PIN1的极化和运输活性。我们还提供证据表明，pin1输出的细胞外生长素对TMK激活和细胞伸长至关重要，从而形成最简单的二元自我调节反馈回路。因此，这些发现为研究生长素信号和运输的潜在自组织回路提供了直接的机制见解，以确保拟南芥植物的正常发育。
{"title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","authors":"Rongfeng Huang, Jiacheng Wang, Mingzeng Chang, Wenxin Tang, Yongqiang Yu, Yi Zhang, Yao Peng, Yanan Wang, Yihan Guo, Ting Lu, Yang Cao, Yewei Zhou, Qinglin Zhang, Yuehong Huang, Angxiao Wu, Luyan Ren, Michelle Gallei, Juan Dong, Haodong Chen, Jun He, Tongda Xu","doi":"10.1016/j.devcel.2025.09.009","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.09.009","url":null,"abstract":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in <em>Arabidopsis</em>.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"214 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145204003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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