Pub Date : 2024-12-01DOI: 10.1016/j.envexpbot.2024.106054
Tania Ho-Plágaro , Jonathan D. Avilés-Cárdenas , Nuria Molinero-Rosales, José M. García-Garrido
Recent research has highlighted the role of GRAS family transcription factors (TFs) in arbuscular mycorrhizal (AM) formation, particularly in tomato plants. This study conducted a functional analysis of the SCL32 GRAS transcription factor SlGRAS38 during arbuscular mycorrhizal formation in tomatoes, confirming its positive regulatory effect on mycorrhiza formation. Experiments reveal that overexpression of SlGRAS38 accelerates mycorrhizal colonization and enhances nutrient uptake, flowering, and fruit yield. Transcriptomic data indicate that SlGRAS38 may function as a new transcriptional regulator during mycorrhization. SlGRAS38 has previously been described as a transcriptional activator in ripening fruits, and here we demonstrate its regulatory role in mycorrhizal roots, suggesting potential cross-talk between these physiological processes. However, the exact mechanisms remain to be fully understood. Further research is needed to explore the interplay between SlGRAS38's roles in mycorrhization and fruit ripening, and to confirm its interaction within transcriptional complexes critical for arbuscule formation. This study underscores the importance of SlGRAS38 in AM development and opens avenues for future investigations into its multifaceted functions.
{"title":"Overexpression of SlGRAS38 in tomato roots accelerates arbuscular mycorrhiza formation","authors":"Tania Ho-Plágaro , Jonathan D. Avilés-Cárdenas , Nuria Molinero-Rosales, José M. García-Garrido","doi":"10.1016/j.envexpbot.2024.106054","DOIUrl":"10.1016/j.envexpbot.2024.106054","url":null,"abstract":"<div><div>Recent research has highlighted the role of GRAS family transcription factors (TFs) in arbuscular mycorrhizal (AM) formation, particularly in tomato plants. This study conducted a functional analysis of the SCL32 GRAS transcription factor SlGRAS38 during arbuscular mycorrhizal formation in tomatoes, confirming its positive regulatory effect on mycorrhiza formation. Experiments reveal that overexpression of <em>SlGRAS38</em> accelerates mycorrhizal colonization and enhances nutrient uptake, flowering, and fruit yield. Transcriptomic data indicate that <em>SlGRAS38</em> may function as a new transcriptional regulator during mycorrhization. SlGRAS38 has previously been described as a transcriptional activator in ripening fruits, and here we demonstrate its regulatory role in mycorrhizal roots, suggesting potential cross-talk between these physiological processes. However, the exact mechanisms remain to be fully understood. Further research is needed to explore the interplay between SlGRAS38's roles in mycorrhization and fruit ripening, and to confirm its interaction within transcriptional complexes critical for arbuscule formation. This study underscores the importance of SlGRAS38 in AM development and opens avenues for future investigations into its multifaceted functions.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106054"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.envexpbot.2024.106050
Samujjal Bhattacharjee , Arun Kumar Mishra
Regulated cell death (RCD) in cyanobacteria is a post-stress phenomenon necessary for stress adaptability and eco-physiology. However, the sequential modulations in cellular processes conferring regulated forms of death remained elusive. In this study, we evaluated morpho-physiological and biochemical determinants of cell death in a time-dependent manner to understand the sequential cellular events during the process. The death was instigated by exposing heterocytous cyanobacterium Anabaena sp. PCC 7120–1 mM H2O2, 250 mM NaCl and sulfur-starvation. A significant changes in the sequences of morpho-physiological and biochemical events were observed in different stress conditions. Upon H2O2 treatment rapid oxidation of cytosol and disintegration of cell envelop was observed prior to DNA fragmentation. On the other hand, salt-stress and sulfur-deprivation induced phosphatidylserine (PS) externalisation, gradual rise in redox potential, and DNA fragmentation prior to membrane disintegration. Furthermore, we analysed the time-dependent expression of five orthocaspases, the putative executioner of cyanobacterial cell death. Out of five, only two orthocaspases anaOC2 and anaOC6 exhibit expression pattern synergistic and coherent with DNA fragmentation in salt treated and sulfur-deficient cells. However, no orthocaspases expression was evident during H2O2 treatment. Considering these parameters, H2O2 induced death was regarded as accidental cell death (ACD), whereas in salt and sulfur stresses mediated two different RCD subroutines, which were orthocaspases-dependent. Overall for the first time, we demonstrated the existence of three death modalities in cyanobacteria based on sequential cellular events and relative expression of orthocaspases.
{"title":"Unveiling abiotic stress-induced cell death modalities in the heterocytous cyanobacterium Anabaena sp. PCC 7120","authors":"Samujjal Bhattacharjee , Arun Kumar Mishra","doi":"10.1016/j.envexpbot.2024.106050","DOIUrl":"10.1016/j.envexpbot.2024.106050","url":null,"abstract":"<div><div>Regulated cell death (RCD) in cyanobacteria is a post-stress phenomenon necessary for stress adaptability and eco-physiology. However, the sequential modulations in cellular processes conferring regulated forms of death remained elusive. In this study, we evaluated morpho-physiological and biochemical determinants of cell death in a time-dependent manner to understand the sequential cellular events during the process. The death was instigated by exposing heterocytous cyanobacterium <em>Anabaena</em> sp. PCC 7120–1 mM H<sub>2</sub>O<sub>2</sub>, 250 mM NaCl and sulfur-starvation. A significant changes in the sequences of morpho-physiological and biochemical events were observed in different stress conditions. Upon H<sub>2</sub>O<sub>2</sub> treatment rapid oxidation of cytosol and disintegration of cell envelop was observed prior to DNA fragmentation. On the other hand, salt-stress and sulfur-deprivation induced phosphatidylserine (PS) externalisation, gradual rise in redox potential, and DNA fragmentation prior to membrane disintegration. Furthermore, we analysed the time-dependent expression of five orthocaspases, the putative executioner of cyanobacterial cell death. Out of five, only two orthocaspases <em>anaOC2</em> and <em>anaOC6</em> exhibit expression pattern synergistic and coherent with DNA fragmentation in salt treated and sulfur-deficient cells. However, no orthocaspases expression was evident during H<sub>2</sub>O<sub>2</sub> treatment. Considering these parameters, H<sub>2</sub>O<sub>2</sub> induced death was regarded as accidental cell death (ACD), whereas in salt and sulfur stresses mediated two different RCD subroutines, which were orthocaspases-dependent. Overall for the first time, we demonstrated the existence of three death modalities in cyanobacteria based on sequential cellular events and relative expression of orthocaspases.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106050"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.envexpbot.2024.106053
R. Juan-Ovejero , J. Castro , F.B. Navarro , J.M. Moreno-Rojas , M.N. Jiménez , A.B. Leverkus , J.I. Querejeta
Functional traits are essential for predicting plant responses to environmental changes, yet understanding within-species trait variability is complex due to multiple influencing factors. Long-lived trees, like the holm oak (Quercus ilex L.), are expected to respond to abiotic variations largely through phenotypic plasticity, so a better understanding of these plastic responses is key to managing climate change impacts on forests. To investigate intra-specific variations in ecophysiological performance under dry Mediterranean conditions, we measured isotopic, nutrient, and growth traits in 240 four-year-old holm oaks from various populations and mother trees grown in a common garden experiment. Contrary to expectations, we found little ecotypic differentiation in isotopic, nutrient, or growth traits among geographically distant populations with contrasting climates, elevations, and lithologies. Leaf Δ¹⁸Oenrichment and δ13C values ranged widely across and within populations, indicating large variability in time-integrated stomatal conductance and water-use efficiency among neighboring oaks grown under the same dry conditions. Both Δ¹⁸Oenrichment and δ13C exhibited negative relationships with leaf C/P and C/K ratios, revealing a trade-off between water-use efficiency and nutrient-use efficiency that was primarily driven by changes in stomatal regulation stringency depending on leaf nutrient status. Holm oaks from all populations were capable of fine-tuning their leaf gas exchange to prioritize efficient use of the most limiting resource for photosynthesis and growth (water versus nutrients). Phosphorus deficiency and stoichiometric N/P imbalance led to lower water-use efficiency and poorer growth. We conclude that all studied holm oak populations possess sufficient phenotypic plasticity and/or genetic diversity to withstand heat and drought stress intensification through adaptive adjustments of their physiological and nutrient traits. Nonetheless, phosphorus fertilization could greatly enhance forest restoration success amid increasing climatic aridity and human-driven N/P imbalance. These findings hold important implications for a better understanding of holm oaks persistence under rapid climate warming and aridification across the Mediterranean region.
{"title":"Large physiological plasticity of water- and nutrient-use traits in Quercus ilex L. within and across populations: Implications for Mediterranean forest persistence under global change","authors":"R. Juan-Ovejero , J. Castro , F.B. Navarro , J.M. Moreno-Rojas , M.N. Jiménez , A.B. Leverkus , J.I. Querejeta","doi":"10.1016/j.envexpbot.2024.106053","DOIUrl":"10.1016/j.envexpbot.2024.106053","url":null,"abstract":"<div><div>Functional traits are essential for predicting plant responses to environmental changes, yet understanding within-species trait variability is complex due to multiple influencing factors. Long-lived trees, like the holm oak (<em>Quercus ilex</em> L.), are expected to respond to abiotic variations largely through phenotypic plasticity, so a better understanding of these plastic responses is key to managing climate change impacts on forests. To investigate intra-specific variations in ecophysiological performance under dry Mediterranean conditions, we measured isotopic, nutrient, and growth traits in 240 four-year-old holm oaks from various populations and mother trees grown in a common garden experiment. Contrary to expectations, we found little ecotypic differentiation in isotopic, nutrient, or growth traits among geographically distant populations with contrasting climates, elevations, and lithologies. Leaf Δ¹⁸O<sub>enrichment</sub> and δ<sup>13</sup>C values ranged widely across and within populations, indicating large variability in time-integrated stomatal conductance and water-use efficiency among neighboring oaks grown under the same dry conditions. Both Δ¹⁸O<sub>enrichment</sub> and δ<sup>13</sup>C exhibited negative relationships with leaf C/P and C/K ratios, revealing a trade-off between water-use efficiency and nutrient-use efficiency that was primarily driven by changes in stomatal regulation stringency depending on leaf nutrient status. Holm oaks from all populations were capable of fine-tuning their leaf gas exchange to prioritize efficient use of the most limiting resource for photosynthesis and growth (water <em>versus</em> nutrients). Phosphorus deficiency and stoichiometric N/P imbalance led to lower water-use efficiency and poorer growth. We conclude that all studied holm oak populations possess sufficient phenotypic plasticity and/or genetic diversity to withstand heat and drought stress intensification through adaptive adjustments of their physiological and nutrient traits. Nonetheless, phosphorus fertilization could greatly enhance forest restoration success amid increasing climatic aridity and human-driven N/P imbalance. These findings hold important implications for a better understanding of holm oaks persistence under rapid climate warming and aridification across the Mediterranean region.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106053"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.envexpbot.2024.106048
Tahir Mahmood , Shoupu He , De Zhu , Hongge Li , Xiaoli Geng , Baojun Chen , Xianpeng Xiong , Xuai Dai , Xiongfeng Ma , Xiongming Du , Guanjing Hu
Drought stress significantly impacts plant growth and agricultural productivity. Elucidating the molecular mechanisms underlying drought stress response and plant tolerance is crucial for developing resilient crops. In Gossypium barbadense (G. barbadense), the specific genetic responses to drought stress remain underexplored. To provide insights into the transcriptomic dynamics and tolerance mechanisms in G. barbadense, we screened a diverse panel of G. barbadense accessions to identify drought-tolerant genotypes and investigate drought-stress responses across root and shoot tissues at two distinct time points. Differentially expressed genes (DEGs) analysis revealed diverse drought-responsive genes across tissue types and treatment time points. Functional enrichment and predictive protein-protein interaction (PPI) network analyses elucidated intricate patterns of drought-stress signaling pathways and transcriptional regulatory mechanisms. These upregulated DEGs were enriched in functional categories such as hormone signal transduction, phosphatidylinositol signaling system, ubiquitin-mediated proteolysis, phenylpropanoid biosynthesis, glutathione metabolism, and carbon metabolism pathways. The PPI network analysis underscores the activation of key signaling genes such as plant U-box E3 ubiquitin ligases (PUBs), protein phosphatase 2 C (PP2Cs), and F-Box genes, as well as transcriptional factors (CBF/NFYA) and various effector genes. These networks revealed the activation of effector genes involved in phenylpropanoid biosynthesis (Thioredoxin like 2–1, 1-Cys), glutathione metabolism (Thioredoxin, GPX6), and carbohydrate/sugar metabolism (GBSSI, AMY1.1). Gene silencing experiments validated the regulatory roles predicted for PUBs and PP2Cs in stress signaling and NFYA transcriptional factor in modifying the plant morphology and physiology to enhance drought tolerance. This research provides critical insights into the genetic signatures of stress signaling and regulatory pathways associated with drought tolerance in G. barbadense. The identified candidate genes are valuable for targeted breeding efforts to enhance drought tolerance and crop yield.
干旱胁迫显著影响植物生长和农业生产力。阐明干旱胁迫响应和植物耐受性的分子机制对培育抗旱性作物至关重要。在巴巴多斯棉（G. barbadense）中，对干旱胁迫的特定遗传反应尚未得到充分的研究。为了深入了解巴巴多斯（G. barbadense）的转录组动力学和耐旱机制，我们筛选了不同种类的巴巴多斯（G. barbadense）材料，以鉴定耐旱基因型，并研究了两个不同时间点根系和茎部组织对干旱胁迫的响应。差异表达基因（DEGs）分析显示，不同组织类型和处理时间点的干旱响应基因存在差异。功能富集和预测蛋白-蛋白相互作用（PPI）网络分析揭示了干旱胁迫信号通路的复杂模式和转录调控机制。这些上调的deg在激素信号转导、磷脂酰肌醇信号系统、泛素介导的蛋白质水解、苯丙素生物合成、谷胱甘肽代谢和碳代谢途径等功能类别中富集。PPI网络分析强调了关键信号基因的激活，如植物U-box E3泛素连接酶（pub）、蛋白磷酸酶2 C （pp2c）和F-Box基因，以及转录因子（CBF/NFYA）和各种效应基因。这些网络揭示了参与苯丙素生物合成（Thioredoxin like 2 - 1,1 - cys）、谷胱甘肽代谢（Thioredoxin, GPX6）和碳水化合物/糖代谢（GBSSI, AMY1.1）的效应基因的激活。基因沉默实验验证了预测的pub和pp2c在胁迫信号中的调控作用以及NFYA转录因子在改变植物形态和生理以增强耐旱性方面的调控作用。这项研究为巴贝登斯干旱耐受性相关的胁迫信号和调控途径的遗传特征提供了重要的见解。所鉴定的候选基因对提高作物抗旱性和产量的有针对性育种工作具有重要价值。
{"title":"Transcriptomic insights into the stress signaling and drought tolerance mechanisms in sea-island cotton (Gossypium barbadense)","authors":"Tahir Mahmood , Shoupu He , De Zhu , Hongge Li , Xiaoli Geng , Baojun Chen , Xianpeng Xiong , Xuai Dai , Xiongfeng Ma , Xiongming Du , Guanjing Hu","doi":"10.1016/j.envexpbot.2024.106048","DOIUrl":"10.1016/j.envexpbot.2024.106048","url":null,"abstract":"<div><div>Drought stress significantly impacts plant growth and agricultural productivity. Elucidating the molecular mechanisms underlying drought stress response and plant tolerance is crucial for developing resilient crops. In <em>Gossypium barbadense</em> (<em>G. barbadense</em>), the specific genetic responses to drought stress remain underexplored. To provide insights into the transcriptomic dynamics and tolerance mechanisms in <em>G. barbadense</em>, we screened a diverse panel of <em>G. barbadense</em> accessions to identify drought-tolerant genotypes and investigate drought-stress responses across root and shoot tissues at two distinct time points. Differentially expressed genes (DEGs) analysis revealed diverse drought-responsive genes across tissue types and treatment time points. Functional enrichment and predictive protein-protein interaction (PPI) network analyses elucidated intricate patterns of drought-stress signaling pathways and transcriptional regulatory mechanisms. These upregulated DEGs were enriched in functional categories such as hormone signal transduction, phosphatidylinositol signaling system, ubiquitin-mediated proteolysis, phenylpropanoid biosynthesis, glutathione metabolism, and carbon metabolism pathways. The PPI network analysis underscores the activation of key signaling genes such as plant U-box E3 ubiquitin ligases (PUBs), protein phosphatase 2 C (PP2Cs), and F-Box genes, as well as transcriptional factors (CBF/NFYA) and various effector genes. These networks revealed the activation of effector genes involved in phenylpropanoid biosynthesis (<em>Thioredoxin like 2–1, 1-Cys</em>), glutathione metabolism (<em>Thioredoxin, GPX6</em>), and carbohydrate/sugar metabolism (<em>GBSSI, AMY1.1</em>). Gene silencing experiments validated the regulatory roles predicted for PUBs and PP2Cs in stress signaling and <em>NFYA</em> transcriptional factor in modifying the plant morphology and physiology to enhance drought tolerance. This research provides critical insights into the genetic signatures of stress signaling and regulatory pathways associated with drought tolerance in <em>G. barbadense</em>. The identified candidate genes are valuable for targeted breeding efforts to enhance drought tolerance and crop yield.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106048"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-24DOI: 10.1016/j.envexpbot.2024.106052
Fengqiong Chen , Yating Wang , Yiyang Liu , Qiusen Chen , Hanlin Liu , Jin Tian , Mengxue Wang , Chunyuan Ren , Qiang Zhao , Fengjun Yang , Jinpeng Wei , Gaobo Yu , Yuxian Zhang
γ-aminobutyric acid (GABA) influences various physiological processes in plants, particularly in carbon and nitrogen metabolism. However, the mechanism underlying carbon (sucrose and unsaturated fatty acid) metabolism in vegetable soybeans was still unknown. In this study, a foliar spray of GABA (10 mM) elevated the level of Ca2+ by up-regulating the expression of calmodulin (GmCaM), which increased glutamate decarboxylase (GAD) activity and boosted endogenous GABA content. This, in turn, enhanced the expression of coding genes of GABA transferase (GmGABA-T) and succinic semialdehyde dehydrogenase (GmSSADH), as well as the activity of GABA transferase (GABA-T), activated the GABA shunt to supply carbon to the tricarboxylic acid (TCA) cycle, thus improved carbon metabolism. The gene expression and activity of sucrose metabolism-related enzymes were also enhanced, leading to the increased accumulation of total soluble sugars, sucrose, glucose, etc. Additionally, exogenous GABA treatment elevated the level of unsaturated fatty acids, including omega-3 arachidonic acid, linoleic acid, alpha-linolenic acid, etc. However, these effects were attenuated by 3-mercaplopropionic acid (3-MP), an inhibitor of GABA synthesis. In summary, exogenous GABA provides a carbon skeleton that promotes the accumulation of sugar and unsaturated fatty acids in vegetable soybean seeds. This research provides a valuable theory for further improving the yield and quality of vegetable soybeans.
{"title":"Exogenous γ-aminobutyric acid (GABA) provides a carbon skeleton to promote the accumulation of sugar and unsaturated fatty acids in vegetable soybean seeds","authors":"Fengqiong Chen , Yating Wang , Yiyang Liu , Qiusen Chen , Hanlin Liu , Jin Tian , Mengxue Wang , Chunyuan Ren , Qiang Zhao , Fengjun Yang , Jinpeng Wei , Gaobo Yu , Yuxian Zhang","doi":"10.1016/j.envexpbot.2024.106052","DOIUrl":"10.1016/j.envexpbot.2024.106052","url":null,"abstract":"<div><div>γ-aminobutyric acid (GABA) influences various physiological processes in plants, particularly in carbon and nitrogen metabolism. However, the mechanism underlying carbon (sucrose and unsaturated fatty acid) metabolism in vegetable soybeans was still unknown. In this study, a foliar spray of GABA (10 mM) elevated the level of Ca<sup>2+</sup> by up-regulating the expression of calmodulin (<em>GmCaM</em>), which increased glutamate decarboxylase (GAD) activity and boosted endogenous GABA content. This, in turn, enhanced the expression of coding genes of GABA transferase (<em>GmGABA-T</em>) and succinic semialdehyde dehydrogenase (<em>GmSSADH</em>), as well as the activity of GABA transferase (GABA-T), activated the GABA shunt to supply carbon to the tricarboxylic acid (TCA) cycle, thus improved carbon metabolism. The gene expression and activity of sucrose metabolism-related enzymes were also enhanced, leading to the increased accumulation of total soluble sugars, sucrose, glucose, etc. Additionally, exogenous GABA treatment elevated the level of unsaturated fatty acids, including omega-3 arachidonic acid, linoleic acid, alpha-linolenic acid, etc. However, these effects were attenuated by 3-mercaplopropionic acid (3-MP), an inhibitor of GABA synthesis. In summary, exogenous GABA provides a carbon skeleton that promotes the accumulation of sugar and unsaturated fatty acids in vegetable soybean seeds. This research provides a valuable theory for further improving the yield and quality of vegetable soybeans.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106052"},"PeriodicalIF":4.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-23DOI: 10.1016/j.envexpbot.2024.106049
Ping Luo , Huanyu Zhang , Yeni Chen , Yongyi Cui , Wen Chen
Drought increasingly constitutes a significant constraint that detrimentally affects plant growth and the productivity of agricultural crops. The bHLHs is pivotal in enabling plants to withstand various abiotic stresses. However, the specific roles of bHLHs in stress remain limited. Here, we explore the role of RhbHLH92 from the Rosa hybrida according to the previous RNA-seq data. The expression of RhbHLH92 was enhanced under several abiotic stress conditions, especially dehydration. RhbHLH92 is located in the nucleus. Enhanced dehydration and drought tolerance were observed in tobacco and rose petals overexpressing RhbHLH92. These genetically modified plants maintained better water balance, showed decreased levels of reactive oxygen species, and exhibited elevated activity of antioxidant enzymes along with increased expression of drought resistance genes compared to WT. Conversely, suppression of RhbHLH92 in rose petals using virus-induced gene silencing (VIGS) heightened their vulnerability to dehydration and reduced the expression of genes associated with stress tolerance. Yeast two-hybrid and BiFC confirmed that RhbHLH92 physically interacts with RhMYB123, a R2R3-type TF. RhMYB123 overexpression in rose petals similarly boosted dehydration tolerance. RhbHLH92 and RhMYB123 could directly bind to the Δ-1-pyrroline-5-carboxylate synthetase (RhP5CS) promoter, the RhbHLH92-RhMYB123 complex led to higher transcript levels of RhP5CS. These findings elucidate a new pathway through which RhbHLH92 enhances drought tolerance in roses, offering potential strategies for the development of drought-resistant crop varieties.
{"title":"RhbHLH92 positively regulates the dehydration tolerance by interacting with RhMYB123 in rose petals (Rosa hybrida)","authors":"Ping Luo , Huanyu Zhang , Yeni Chen , Yongyi Cui , Wen Chen","doi":"10.1016/j.envexpbot.2024.106049","DOIUrl":"10.1016/j.envexpbot.2024.106049","url":null,"abstract":"<div><div>Drought increasingly constitutes a significant constraint that detrimentally affects plant growth and the productivity of agricultural crops. The bHLHs is pivotal in enabling plants to withstand various abiotic stresses. However, the specific roles of bHLHs in stress remain limited. Here, we explore the role of <em>RhbHLH92</em> from the <em>Rosa hybrida</em> according to the previous RNA-seq data. The expression of <em>RhbHLH92</em> was enhanced under several abiotic stress conditions, especially dehydration. RhbHLH92 is located in the nucleus. Enhanced dehydration and drought tolerance were observed in tobacco and rose petals overexpressing <em>RhbHLH92.</em> These genetically modified plants maintained better water balance, showed decreased levels of reactive oxygen species, and exhibited elevated activity of antioxidant enzymes along with increased expression of drought resistance genes compared to WT. Conversely, suppression of <em>RhbHLH92</em> in rose petals using virus-induced gene silencing (VIGS) heightened their vulnerability to dehydration and reduced the expression of genes associated with stress tolerance. Yeast two-hybrid and BiFC confirmed that RhbHLH92 physically interacts with RhMYB123, a R2R3-type TF. <em>RhMYB123</em> overexpression in rose petals similarly boosted dehydration tolerance. RhbHLH92 and RhMYB123 could directly bind to the <em>Δ-1-pyrroline-5-carboxylate synthetase</em> (<em>RhP5CS</em>) promoter, the RhbHLH92-RhMYB123 complex led to higher transcript levels of <em>RhP5CS</em>. These findings elucidate a new pathway through which <em>RhbHLH92</em> enhances drought tolerance in roses, offering potential strategies for the development of drought-resistant crop varieties.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106049"},"PeriodicalIF":4.5,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142720402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-22DOI: 10.1016/j.envexpbot.2024.106051
Jia Hu , Siyuan Nan , Lieding Zhou , Changhong Yu , Yajing Li , Kai Zhao , Shuhui Du , Youzhi Han , Shengji Wang
Poplar (Populus L.) is a fast-growing economic timber plant that is susceptible to salt stress. Here, PagbZIP75 (Potri.014G120800), which was isolated from 84 K poplar and upregulated in response to salt treatment, was investigated by generating overexpression (OE) and repression (RNAi) transgenic lines to elucidate its role in poplar salt stress tolerance through molecular and physiological approaches. PagbZIP75 localized in the nucleus and cell membrane but lacked transcriptional activation activity in yeast cells. Expression pattern analysis revealed that PagbZIP75 was induced by salt stress, peaking at 12 hours in roots and stems and 24 hours in leaves. Under salt stress, OE exhibited enhanced growth and a more robust root system compared to non-transgenic 84 K poplar (WT) and RNAi. DAB and NBT staining results demonstrated lower levels of reactive oxygen species (ROS) in OE leaves, alongwith reduced electrolyte leakage rate and superoxide anion (O2-) content, while the proline content and superoxide dismutase (SOD) activity were significantly elevated under salt stress. Based on the RNA-seq data, multilayered hierarchical gene regulatory network (ML-hGRN) around bZIP75 was illustrated and indicated that PagbZIP75 was induced by ABA hormone along with 10 salt-related co-expressed genes. Yeast one-hybrid (Y1H) experiments indicated the binding of PagAREB1 protein to the 0–208 bp upstream fragments of PagbZIP75, and dual luciferase assays (LUC) confirmed a negative interaction between AREB1 and bZIP75. Overall, this study provides a theoretical foundation for the enhancement of poplar salt tolerance by PagbZIP75 through the reduction of ROS accumulation via ABA signaling.
杨树（Populus L.）是一种快速生长的经济用材植物，易受盐胁迫影响。在此，研究人员通过产生过表达（OE）和抑制（RNAi）转基因品系，研究了从 84 K 杨树中分离并在盐处理中上调的 PagbZIP75（Potri.014G120800），以通过分子和生理方法阐明其在杨树耐盐胁迫中的作用。PagbZIP75 定位于细胞核和细胞膜，但在酵母细胞中缺乏转录激活活性。表达模式分析显示，PagbZIP75受到盐胁迫的诱导，在根和茎的12小时和叶的24小时达到峰值。在盐胁迫下，与非转基因 84 K 杨树（WT）和 RNAi 相比，OE 表现出更强的生长能力和更健壮的根系。DAB 和 NBT 染色结果表明，在盐胁迫下，OE 叶片中活性氧（ROS）水平降低，电解质渗漏率和超氧阴离子（O2-）含量减少，而脯氨酸含量和超氧化物歧化酶（SOD）活性显著升高。基于RNA-seq数据，绘制了围绕bZIP75的多层分级基因调控网络（ML-hGRN），表明PagbZIP75与10个与盐相关的共表达基因一起被ABA激素诱导。酵母单杂交（Y1H）实验表明，PagAREB1蛋白与PagbZIP75上游0-208 bp片段结合，双荧光素酶测定（LUC）证实了AREB1与bZIP75之间的负作用。总之，本研究为 PagbZIP75 通过 ABA 信号减少 ROS 积累增强杨树耐盐性提供了理论依据。
{"title":"PagbZIP75 decreases the ROS accumulation to enhance salt tolerance of poplar via the ABA signaling","authors":"Jia Hu , Siyuan Nan , Lieding Zhou , Changhong Yu , Yajing Li , Kai Zhao , Shuhui Du , Youzhi Han , Shengji Wang","doi":"10.1016/j.envexpbot.2024.106051","DOIUrl":"10.1016/j.envexpbot.2024.106051","url":null,"abstract":"<div><div>Poplar (<em>Populus</em> L.) is a fast-growing economic timber plant that is susceptible to salt stress. Here, <em>PagbZIP75</em> (<em>Potri.014G120800</em>), which was isolated from 84 K poplar and upregulated in response to salt treatment, was investigated by generating overexpression (OE) and repression (RNAi) transgenic lines to elucidate its role in poplar salt stress tolerance through molecular and physiological approaches. PagbZIP75 localized in the nucleus and cell membrane but lacked transcriptional activation activity in yeast cells. Expression pattern analysis revealed that <em>PagbZIP75</em> was induced by salt stress, peaking at 12 hours in roots and stems and 24 hours in leaves. Under salt stress, OE exhibited enhanced growth and a more robust root system compared to non-transgenic 84 K poplar (WT) and RNAi. DAB and NBT staining results demonstrated lower levels of reactive oxygen species (ROS) in OE leaves, alongwith reduced electrolyte leakage rate and superoxide anion (O<sub>2</sub><sup>-</sup>) content, while the proline content and superoxide dismutase (SOD) activity were significantly elevated under salt stress. Based on the RNA-seq data, multilayered hierarchical gene regulatory network (ML-hGRN) around <em>bZIP75</em> was illustrated and indicated that <em>PagbZIP75</em> was induced by ABA hormone along with 10 salt-related co-expressed genes. Yeast one-hybrid (Y1H) experiments indicated the binding of PagAREB1 protein to the 0–208 bp upstream fragments of <em>PagbZIP75</em>, and dual luciferase assays (LUC) confirmed a negative interaction between AREB1 and bZIP75. Overall, this study provides a theoretical foundation for the enhancement of poplar salt tolerance by <em>PagbZIP75</em> through the reduction of ROS accumulation via ABA signaling.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106051"},"PeriodicalIF":4.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142720401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Light and nutrients are vital environmental factors shaping plant growth and metabolism, yet their interactive effects on leaf dynamics, nitrogen assimilation, and cellular energetics remain largely unexplored. We aimed to investigate these processes in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze under two light levels; high-light (HL, 0 % shade) and low-light (LL, 50 % shade) combined with four nutrient levels; low (NPK1, 40 mg kg−1), moderate (NPK2, 80 mg kg−1), high (NPK3, 120 mg kg−1) and very high (NPK4, 160 mg kg−1). High-light conditions and high-nutrient levels (HL-NPK3) synergistically enhanced leaf mass area by 44 % with net photosynthesis rates and nitrate reductase activity increasing by up to 17.62 ± 0.89 µmol CO2 m−2 s−1 and 0.34 ± 0.02 μmol NO2 cm−2 h−1 each. Low-light and moderate-nutrient levels (LL-NPK2) triggered a 42 % increase in specific leaf area and threefold higher photosynthetic nitrogen use efficiency. Unexpectedly, high-light and moderate-nutrient levels (HL-NPK2) elicited peak vacuolar H+-ATPase and H+-pyrophosphatase activities at 15.6 % and 53.1 % each. This study also found significant positive correlations between chlorophyll content, nitrate reductase (r = 0.62, P < 0.01), and vacuolar H+-ATPase activity (r = 0.58, P < 0.01), suggesting a mechanism for maintaining high photosynthetic capacity and efficient nitrogen assimilation. The clustering of leaf area index, specific leaf area, and photosynthetic nitrogen use efficiency (similarity of > 70 %) suggests optimized leaf structure and nitrogen use in light-limited but nutrient-rich environments. Our findings show how A. rugosa adjusts its physiology in response to environmental conditions, with implications for understanding plant adaptation and improving cultivation practices.
{"title":"Light-nutrient interaction orchestrates leaf dynamics, nitrogen assimilation, and cellular energetics in Agastache rugosa (Fisch. & C.A.Mey.) Kuntze","authors":"Khairul Azree Rosli , Azizah Misran , Latifah Saiful Yazan , Puteri Edaroyati Megat Wahab","doi":"10.1016/j.envexpbot.2024.106044","DOIUrl":"10.1016/j.envexpbot.2024.106044","url":null,"abstract":"<div><div>Light and nutrients are vital environmental factors shaping plant growth and metabolism, yet their interactive effects on leaf dynamics, nitrogen assimilation, and cellular energetics remain largely unexplored. We aimed to investigate these processes in <em>Agastache rugosa</em> (Fisch. & C.A.Mey.) Kuntze under two light levels; high-light (HL, 0 % shade) and low-light (LL, 50 % shade) combined with four nutrient levels; low (NPK1, 40 mg kg<sup>−1</sup>), moderate (NPK2, 80 mg kg<sup>−1</sup>), high (NPK3, 120 mg kg<sup>−1</sup>) and very high (NPK4, 160 mg kg<sup>−1</sup>). High-light conditions and high-nutrient levels (HL-NPK3) synergistically enhanced leaf mass area by 44 % with net photosynthesis rates and nitrate reductase activity increasing by up to 17.62 ± 0.89 µmol CO<sub>2</sub> m<sup>−2</sup> s<sup>−1</sup> and 0.34 ± 0.02 μmol NO<sub>2</sub> cm<sup>−2</sup> h<sup>−1</sup> each. Low-light and moderate-nutrient levels (LL-NPK2) triggered a 42 % increase in specific leaf area and threefold higher photosynthetic nitrogen use efficiency. Unexpectedly, high-light and moderate-nutrient levels (HL-NPK2) elicited peak vacuolar H<sup>+</sup>-ATPase and H<sup>+</sup>-pyrophosphatase activities at 15.6 % and 53.1 % each. This study also found significant positive correlations between chlorophyll content, nitrate reductase (r = 0.62, P < 0.01), and vacuolar H<sup>+</sup>-ATPase activity (r = 0.58, P < 0.01), suggesting a mechanism for maintaining high photosynthetic capacity and efficient nitrogen assimilation. The clustering of leaf area index, specific leaf area, and photosynthetic nitrogen use efficiency (similarity of > 70 %) suggests optimized leaf structure and nitrogen use in light-limited but nutrient-rich environments. Our findings show how <em>A</em>. <em>rugosa</em> adjusts its physiology in response to environmental conditions, with implications for understanding plant adaptation and improving cultivation practices.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"229 ","pages":"Article 106044"},"PeriodicalIF":4.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142745156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-19DOI: 10.1016/j.envexpbot.2024.106045
Xiulong Zhang , Fanglan Li , Lulu Xie , Weikai Bao
Functional relationships between photosynthetic behavior and hydraulic properties are essential to characterize plant drought adaptation strategies. However, little is known about such relationships in response to varying rock fragment content (RFC), which could induce severe soil water deficit. We evaluated the leaf hydraulic properties and the timing of diurnal depression of photosynthesis in three xerophytic shrub species grown under different RFC levels (0, 25, 50, 75 % v v−1). We found that studied species grown in 25 % RFC soil conditions had higher leaf hydraulic conductance (Kleaf) and reached maximum photosynthetic rate (Amax) in the morning, while those grown in 75 % RFC soil conditions had lower Kleaf, but reached their Amax in the afternoon. In addition, species in 75 % RFC soil conditions also exhibited low leaf hydraulic vulnerability and narrow leaf hydraulic safety margins. Our results indicate that RFC modifies the diurnal gas exchange dynamics of xerophytic species by decreasing leaf hydraulic vulnerability and hydraulic safety margins. Specifically, species surviving in 75 % RFC soils are less vulnerable to drought induced water loss, and carbon assimilation depression were later than in 25 % or 0 % RFC soil conditions. However, when faced with severe drought, these species with latter CO2 uptake depression are at higher risk of hydraulic failure, because their safety margins are relatively narrow. Our results contribute to the knowledge of drought adaptation strategies in xerophytic species native to dry-hot rocky mountains.
{"title":"Rocky soils alter the diurnal photosynthetic behavior of xerophytic species by regulating hydraulic properties","authors":"Xiulong Zhang , Fanglan Li , Lulu Xie , Weikai Bao","doi":"10.1016/j.envexpbot.2024.106045","DOIUrl":"10.1016/j.envexpbot.2024.106045","url":null,"abstract":"<div><div>Functional relationships between photosynthetic behavior and hydraulic properties are essential to characterize plant drought adaptation strategies. However, little is known about such relationships in response to varying rock fragment content (RFC), which could induce severe soil water deficit. We evaluated the leaf hydraulic properties and the timing of diurnal depression of photosynthesis in three xerophytic shrub species grown under different RFC levels (0, 25, 50, 75 % v v<sup>−1</sup>). We found that studied species grown in 25 % RFC soil conditions had higher leaf hydraulic conductance (<em>K</em><sub>leaf</sub>) and reached maximum photosynthetic rate (<em>A</em><sub>max</sub>) in the morning, while those grown in 75 % RFC soil conditions had lower <em>K</em><sub>leaf</sub>, but reached their <em>A</em><sub>max</sub> in the afternoon. In addition, species in 75 % RFC soil conditions also exhibited low leaf hydraulic vulnerability and narrow leaf hydraulic safety margins. Our results indicate that RFC modifies the diurnal gas exchange dynamics of xerophytic species by decreasing leaf hydraulic vulnerability and hydraulic safety margins. Specifically, species surviving in 75 % RFC soils are less vulnerable to drought induced water loss, and carbon assimilation depression were later than in 25 % or 0 % RFC soil conditions. However, when faced with severe drought, these species with latter CO<sub>2</sub> uptake depression are at higher risk of hydraulic failure, because their safety margins are relatively narrow. Our results contribute to the knowledge of drought adaptation strategies in xerophytic species native to dry-hot rocky mountains.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106045"},"PeriodicalIF":4.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-19DOI: 10.1016/j.envexpbot.2024.106047
Sumitra Pantha , Benjamin Kilian , Hakan Özkan , Frederike Zeibig , Michael Frei
Drought stress negatively influences the growth, development, and grain yield of wheat by disrupting its morphological, physiological, and biochemical processes. This study examined the effects of drought stress during the stem elongation and anthesis developmental stages of species within the Triticum genus along with their drought adaptation mechanisms under fully watered and drought conditions. We tested the following two hypotheses: (1) drought tolerance mechanisms for osmotic and stomatal regulation that lead to oxidative stress are correlated between the stem elongation and anthesis stages and affect grain yield loss, and (2) compared with modern cultivars, wild wheat cultivars exhibit greater drought tolerance. To test these hypotheses, a greenhouse pot experiment was conducted using 17 genotypes of wild wheat relatives and landraces, with modern cultivars included for comparison. Drought stress was induced during the stem elongation and anthesis stages until the average soil moisture was approximately 15 % and 18 %, respectively, of the pot’s water holding capacity. The soil moisture was maintained at 80–90 % for the fully watered treatment. An examination of physiological and biochemical traits revealed that drought significantly reduced stomatal conductance (gsw) and relative water content (RWC) during both developmental stages. However, significant increases occurred in the malondialdehyde (MDA) content during both stages and in the proline content during the anthesis stage. Drought stress significantly decreased the number of days to heading and anthesis, indicating that drought escape occurs under severe drought stress. Furthermore, drought significantly decreased morphological and yield-related traits, with the greatest reduction (51 %) occurring in grain yield. Weakly significant positive associations of biochemical and some physiological traits between the stem elongation and anthesis stages partially confirmed our first hypothesis, whereas our results relating to the second hypothesis were inconclusive. We observed genotype-dependent responses to drought stress during both stages for various measured traits. No associations of RWC, proline, or MDA with grain yield were found. However, stomatal conductance was negatively correlated with grain yield under drought stress at the anthesis stage. Certain wild wheat genotypes and landraces exhibited drought avoidance, escape, and tolerance mechanisms, which positively contributed to grain yield. We identified T. monococcum subsp. sinskajae, T. boeoticum and T. dicoccoides as the most drought-tolerant genotypes. The findings of this study provide important insight for understanding the drought adaptation traits and their use in wheat breeding programs.
{"title":"Physiological and biochemical changes induced by drought stress during the stem elongation and anthesis stages in the Triticum genus","authors":"Sumitra Pantha , Benjamin Kilian , Hakan Özkan , Frederike Zeibig , Michael Frei","doi":"10.1016/j.envexpbot.2024.106047","DOIUrl":"10.1016/j.envexpbot.2024.106047","url":null,"abstract":"<div><div>Drought stress negatively influences the growth, development, and grain yield of wheat by disrupting its morphological, physiological, and biochemical processes. This study examined the effects of drought stress during the stem elongation and anthesis developmental stages of species within the <em>Triticum</em> genus along with their drought adaptation mechanisms under fully watered and drought conditions. We tested the following two hypotheses: (1) drought tolerance mechanisms for osmotic and stomatal regulation that lead to oxidative stress are correlated between the stem elongation and anthesis stages and affect grain yield loss, and (2) compared with modern cultivars, wild wheat cultivars exhibit greater drought tolerance. To test these hypotheses, a greenhouse pot experiment was conducted using 17 genotypes of wild wheat relatives and landraces, with modern cultivars included for comparison. Drought stress was induced during the stem elongation and anthesis stages until the average soil moisture was approximately 15 % and 18 %, respectively, of the pot’s water holding capacity. The soil moisture was maintained at 80–90 % for the fully watered treatment. An examination of physiological and biochemical traits revealed that drought significantly reduced stomatal conductance (gsw) and relative water content (RWC) during both developmental stages. However, significant increases occurred in the malondialdehyde (MDA) content during both stages and in the proline content during the anthesis stage. Drought stress significantly decreased the number of days to heading and anthesis, indicating that drought escape occurs under severe drought stress. Furthermore, drought significantly decreased morphological and yield-related traits, with the greatest reduction (51 %) occurring in grain yield. Weakly significant positive associations of biochemical and some physiological traits between the stem elongation and anthesis stages partially confirmed our first hypothesis, whereas our results relating to the second hypothesis were inconclusive. We observed genotype-dependent responses to drought stress during both stages for various measured traits. No associations of RWC, proline, or MDA with grain yield were found. However, stomatal conductance was negatively correlated with grain yield under drought stress at the anthesis stage. Certain wild wheat genotypes and landraces exhibited drought avoidance, escape, and tolerance mechanisms, which positively contributed to grain yield. We identified <em>T. monococcum</em> subsp. <em>sinskajae</em>, <em>T. boeoticum</em> and <em>T. dicoccoides</em> as the most drought-tolerant genotypes. The findings of this study provide important insight for understanding the drought adaptation traits and their use in wheat breeding programs.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"228 ","pages":"Article 106047"},"PeriodicalIF":4.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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