Environmental toxicology and pharmacology最新文献

Among cereal contaminants, mycotoxins are of concern due to their importance in terms of food and feed safety. The difficulty in establishing a diagnosis for mycotoxicosis relies on the fact that the effects are most often subclinical for chronic exposure and the most common scenario is multi-contamination by various toxins. Mycotoxin co-occurrence is a major food safety concern as additive or even synergic toxic impacts may occur, but also regarding current regulations as they mainly concern individual mycotoxin levels in specific foods and feed in the food chain. However, due to the large number of possible mycotoxin combinations, there is still limited knowledge on co-exposure toxicity data, which depends on several parameters. In this context, this systematic review aims to provide an overview of the toxic effects of two regulated mycotoxins, namely ochratoxin A and fumonisin B1. This review focused on the 2012–2022 period and analysed the occurrence in Europe of the selected mycotoxins in different food matrices (cereals and cereal-derived products), and their toxic impact, alone or in combination, on in vitro intestinal and hepatic human cells. To better understand and evaluate the associated risks, further research is needed using new approach methodologies (NAM), such as in vitro 3D models.

Key contribution

Cereals and their derived products are the most important food source for humans and feed for animals worldwide. This manuscript is a state of the art review of the literature over the last ten years on ochratoxin A and fumonisin B1 mycotoxins in these products in Europe as well as their toxicological effects, alone and in combination, on human cells. Future perspectives and some challenges regarding the assessment of toxicological effects of mycotoxins are also discussed.

We evaluated whether thymol (THY) (30 mg/kg b.wt) could relieve the adverse effects of the neonicotinoid insecticide imidacloprid (IMD) (22.5 mg/kg b.wt) on the liver in a 56-day oral experiment and the probable underlying mechanisms. THY significantly suppressed the IMD-associated increase in hepatic enzyme leakage. Besides, the IMD-induced dyslipidemia was considerably corrected by THY. Moreover, THY significantly repressed the IMD-induced hepatic oxidative stress, lipid peroxidation, DNA damage, and inflammation. Of note, the Feulgen, mercuric bromophenol blue, and PAS-stained hepatic tissue sections analysis declared that treatment with THY largely rescued the IMD-induced depletion of the DNA, total proteins, and polysaccharides. Moreover, THY treatment did not affect the NF-kB p65 immunoexpression but markedly downregulated the Caspase-3 in the hepatocytes of the THY+IMD-treated group than the IMD-treated group. Conclusively, THY could efficiently protect against IMD-induced hepatotoxicity, probably through protecting cellular macromolecules and antioxidant, antiapoptotic, and anti-inflammatory activities.

Endocrine disrupting chemicals or carcinogens have been known for decades for their endocrine signal disruption. Endocrine disrupting chemicals are a serious concern and they have been included in the top priority toxicants and persistent organic pollutants. Therefore, researchers have been working for a long time to understand their mechanisms of interaction in different human organs. Several reports are available about the carcinogen potential of these chemicals. The presented review is an endeavor to understand the hazard identification associated with endocrine disrupting carcinogens in relation to the human body. The paper discusses the major endocrine disrupting carcinogens and their potency for carcinogenesis. It discusses human exposure, route of entry, carcinogenicity and mechanisms. In addition, the paper discusses the research gaps and bottlenecks associated with the research. Moreover, it discusses the limitations associated with the analytical techniques for detection of endocrine disrupting carcinogens.

Plant growth regulators (PGRs) are increasingly used to promote sustainable agriculture, but their unregulated use raises concerns about potential environmental risks. Indole-3-acetic acid (IAA), a commonly used PGR, has been the subject of research on its developmental toxicity in the in-vivo zebrafish model. IAA exposure to zebrafish embryos caused oxidative stress, lipid peroxidation, and cellular apoptosis. The study also revealed that critical antioxidant genes including sod, cat, and bcl2 were downregulated, while pro-apoptotic genes such as bax and p53 were upregulated. IAA exposure also hampered normal cardiogenesis by downregulating myl7, amhc, and vmhc genes and potentially influencing zebrafish neurobehavior. The accumulation of IAA was confirmed by HPLC analysis of IAA-exposed zebrafish tissues. These findings underscore the need for further study on the potential ecological consequences of IAA use and the need for sustainable agricultural practices.

Biochar is currently garnering interest as an alternative to commercial fertilizer and as a tool to counteract global warming. However, its use is increasingly drawing attention, particularly concerning the fine dust that can be developed during its manufacture, transport, and use. This work aimed to assess the toxicity of fine particulate Biochar (<PM₁₀) via in-vitro and in-vivo experiments as a first step for the evaluation of toxicity values. As in-vitro experiments, cell lines showed inhibition of proliferation following the reduction of expression genes involved in cell cycle control, increase in the production of ROS and IL-8, and decrease in intracellular ATP. In-vivo rat exposure induced hyperemia, edema, and inflammatory phenomena with infiltrations of neutrophil granulocytes and macrophages at the alveolar and bronchiolar levels. Both in-vitro and in-vivo studies highlighted how exposure to Biochar particulates leads to an inflammatory condition and oxidative stress.

Microplastics are pollutants of emerging concern and the aquatic biota consumes microplastics (MPs), which has a range of toxicological and environmental effects on aquatic organisms that are not the intended targets. The current study looked into how weathered polyethylene (wPE) MPs affected Danio albolineatus immunological and haematological markers. In this experiment, fish of both sexes were placed in control and exposure groups, and they were exposed for 40 d at the sublethal level (1 μg L^-1) of fragmented wPE, which contained 1074 ± 52 MPs per litre. Similarly, fish exposed to wPE MPs showed significant modifications in lysozyme, antimicrobial, and antiprotease activity, as well as differential counts. Results of the present study show that the male fish were more susceptible than female fish after 40 d of chronic exposure. Further studies are needed to ascertain how the innate and humoral immune systems of the fish respond to MPs exposure.

Studies reported that continuous application of glyphosate can cause disturbance in aquatic/terrestrial environments. As such, the objective of this study is to discuss the risk of exposure to the herbicide in drinking water and to assess the oxidative stress in the consumers rural populations of Casimiro de Abreu/ RJ and Paraguaçu/ MG, Brazil. For this, water samples (n=69) were analysed from the home of volunteers, by FMOC derivatizing- LC-FLD method. The oxidative stress was analysed determining lipid peroxidation (MAD) and defense enzymes (SOD and CAT) in serum samples from rural population (n=42) compared to urban residents (n= 42). Results of the analysis from drinking water, despite the low and moderate risk, by the hazard quotient (HQ), revealed that the population is environmentally exposed to the glyphosate. The relevant findings showed that is important to implement monitoring/ biomonitoring programs to prevent pollution and toxic effects in the rural populations.

The present investigation focuses on the identification of popular PhACs in roots, leaves and rice grains, which are cultivated in soil irrigated with waters and wastewater. The present study reveals the presence of PhACs in rice grains from different brands which are available in the current market, which has thus motivated these experiments. The rice plants were cultivated in garden containers and irrigated with three different water sources. All PhAC compounds were recovered within an 89–111 % range using the extraction technique, reproducibility, and sensitivity (LOQ <25 µg/g). Further, PhAC compounds were identified using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QqTOF-MS). Interestingly, several PhAC compounds were detected in rice grains, aligning with hypotheses and findings from published literature. A total of ten (10) PhACs were found in the root, leaf, and rice grain of the 20 popular PhACs that were targeted. The annual exposure and medical dose equivalent for individual PhACs was negligible. According to our knowledge, this study is the first to show the accumulation of several categories (cocktail) of PhACs in rice grains and show the approximate human health risk assessment by its consumption. The study's results provide valuable insights for researchers, policymakers, and agricultural practitioners working on sustainable agriculture and public health.

Lead toxicity poses a significant environmental concern linked to diverse health issues. This study explores the potential mitigating effects of resveratrol on lead-induced toxicity in Drosophila melanogaster. Adult fruit flies, aged three days, were orally exposed to lead (60 mg/L), Succimer (10 mg), and varying concentrations of resveratrol (50, 100, and 150 mg). The investigation encompassed the assessment of selected biological parameters, biochemical markers, oxidative stress indicators, and antioxidant enzymes. Resveratrol exhibited a dose-dependent enhancement of egg-laying, eclosion rate, filial generation output, locomotor activity, and life span in D. melanogaster, significantly to 150 mg of diet. Most of the investigated biochemical parameters were significantly rescued in lead-exposed fruit flies when co-treated with resveratrol (p < 0.05). However, oxidative stress remained unaffected by resveratrol. The findings suggest that resveratrol effectively protects against lead toxicity in Drosophila melanogaster and may hold therapeutic potential as an agent for managing lead poisoning in humans.

Pharmaceuticals released from municipal effluents discharges pose a risk to aquatic organisms. The toxicity of 5 pharmaceuticals with distinct therapeutic actions were assessed in rainbow trout: olanzapine (antipsychotic), erythromycin (antibiotic), mycophenoate (immunosuppression), pinaverium (anti-inflammatory) and trazodone (sedative). Juveniles were exposed to these drugs for 96 h at concentrations between 64 µg/L up to 40 mg/L to reach lethality. Survival was determined and a suite of biomarkers was analyzed for drug biotransformation, oxidative stress/damage and metabolic activity at sublethal concentrations. The data revealed the following toxicity: olanzapine >trazodone>mycophenolate>pinaverium∼erythromycin based on mortality. The data also revealed that toxicity was associated to mass, pKa and hydrophobicity and the following sublethal effects: GST, LPO and DNA strand breaks. Pharmaceuticals with lower molecular weight, physiological pKa, moderate hydrophobicity, low biotransformation and DNA strand breaks were generally more toxic to fish. However, this should be considered as a general guide in identifying toxic pharmaceuticals in non-target organisms.