European Journal of Nutrition最新文献

Purpose: A transition to more plant-rich diets is an effective way to reduce the climate impact of a diet. Using a whole-diet approach, we studied how partial replacement of animal-sourced with plant-sourced proteins affected the dietary climate impact while simultaneously considering diet-related health biomarkers.

Methods: In a 12-week randomised controlled trial, 107 women and 29 men were assigned into three diet groups (ANIMAL, 50/50, PLANT) with animal-to-plant-protein ratios of 70/30, 50/50, and 30/70, respectively. Life-cycle-assessment-based coefficients for foods were used to assess the climate impact of the diet groups, based on four-day food records. Correlations between climate impact and biomarkers were assessed.

Results: The climate impact (CO₂ eq.) for PLANT was 3.32 kg per day, 3.05 kg per 2,000 kcal, and 0.04 kg per gram of protein, for 50/50 4.34, 4.20, and 0.05 kg, and for ANIMAL 4.93, 4.94, and 0.06 kg, respectively (p < 0.05 for all except ANIMAL vs. 50/50 /g protein and /2,000 kcal). Climate impact correlated weakly positively with colorectal cancer risk markers and a positive status of bone turnover, but not with cardiometabolic risk markers. Animal-based iron intake and climate impact (per 2,000 kcal) had a strong positive correlation 0.70 C.I. [0.60, 0.77], while saturated fat (0.29 [0.13, 0.44]) and calcium (0.37, [0.22, 0.51]) intake had a weak positive correlation, and fibre intake (- 0.37, [- 0.51, - 0.21]) a weak negative correlation with climate impact.

Conclusion: Replacing animal-sourced proteins with plant-sourced proteins reduced the climate impact of the diet. The relationship between climate impact and biomarkers was more ambiguous indicated by both beneficial and harmful indicators within lower climate impacts.

Clinical trial registry: NCT03206827; registration date: 2017-06-30.

Purpose: It is generally believed that refined grains lack nutritional value compared to whole grains. The objective of this study was to investigate whether refined highland barley (RHB) holds the potential to combat obesity-associated insulin resistance.

Methods: Thirty-two male 6-week-old C57BL/6J mice were randomly divided into four groups fed with a normal chow diet, a high-fat diet (HFD), a 30% RHB supplemented HFD diet, and a 30% whole-grain highland barley (WGHB) supplemented HFD diet. We examined the anti-obesity and anti-insulin resistance effects of RHB and compared them with WGHB in mice.

Results: RHB intervention effectively improved obesity and insulin resistance, enhanced the intestinal mucosal barrier, and reduced inflammation. Moreover, it promoted the abundance of beneficial gut bacteria such as Akkermansia, Bifidobacterium, Lachnospiraceae_NK4A136_group, Lachnospiraceae_UCG-001, Alloprevotella, and increased the production of short-chain fatty acids (SCFAs) in faeces. Additionally, RHB intervention modulated liver gene transcription, downregulating inflammatory genes like IRF3/7, STAT1/2, NLRP3, and TLR2.

Conclusions: RHB could effectively alleviate obesity-related insulin resistance by targeting gut microbiota and liver transcriptomics, and its beneficial impacts are comparable to those of WGHB.

Purpose: Recent studies indicate that shift work may affect workers' eating habits and overall well-being. This study aimed to assess differences in eating patterns, sleep quality, and mental health between Italian shift and non-shift workers, with a focus on individual chronotype and the type of shift work (day vs. night shift).

Methods: The cross-sectional study involved 322 subjects (166 shift and 156 non-shift workers). Eating habits were evaluated using a 7-day diary and the Medi-Lite questionnaire. Sleep quality was assessed with the Pittsburgh Sleep Quality Index (PSQI), and mental health with the Depression Anxiety Stress Scales (DASS). Individual chronotype was defined using the Morningness-Eveningness Questionnaire.

Results: No significant differences in daily energy, macronutrient, and micronutrient intake between the two groups, nor in the temporal pattern of eating. However, shift workers had significantly (p < 0.05) lower adherence to the Mediterranean diet (MD) (7.6 ± 2.3 vs 8.1 ± 2.2) compared to non-shift workers. Shift workers also reported significantly poorer sleep quality (mean PSQI score 7.6 ± 3.7 vs. 5.8 ± 3.0) and higher levels of anxiety and stress symptoms. Among shift workers, those with an evening chronotype had significantly lower MD adherence than those with a morning chronotypes. Additionally, night shift workers experienced more sleep disturbances compared to day ones.

Conclusion: Shift workers reported lower MD adherence, poorer sleep quality, and a higher prevalence of anxiety and stress symptoms compared to a similar group of non-shift workers. Evening chronotypes and night shift work were associated with worse eating habits and sleep quality.

Purpose: High consumption of ultra-processed food (UPF) is associated with an increased risk of developing chronic diseases. Inflammation may be one of the underlying mechanisms behind this association. However, only a limited number of studies have investigated the association between UPF consumption and a few selected inflammation biomarkers, yielding inconsistent results. This study aimed to assess the cross-sectional association between UPF consumption (as a whole and 10 sub-categories), and 78 circulating proteins related to inflammation.

Methods: The present study included 1594 adult participants from the STANISLAS cohort. UPF consumption was estimated using the NOVA classification, and linear regression models were used to assess their association with circulating proteins.

Results: UPFs accounted for 28% of the total energy intake and 5.7 servings on average per day. In the unadjusted model, 15 circulating proteins had a significant association with UPF consumption. After adjustment, only (FGF-19) was significantly associated with UPF consumption (β =  - 0.02[- 0.03; - 0.003]).

Conclusion: UPF consumption was negatively associated with Fibroblast Growth Factor 19 (FGF-19) serum levels. When considering UPF sub-categories, no circulating proteins were associated with dairy products and dairy desserts. Of note, circulating proteins were differentially associated depending on the sub-category of UPF. Further studies are needed to better understand the link between UPF and inflammation.

Purpose: A high-fructose diet is supposed to induce the so-called metabolic syndrome, associated with increased fat deposition in adipose tissue. Physical exercise may counteract the induction of the metabolic syndrome. The present study aims to investigate the effect of voluntary physical activity (VPA) on cGMP-mediated lipolysis in retroperitoneal adipose tissue in a metabolic syndrome model induced in rats by a high-fructose diet.

Methods: Male Sprague-Dawley rats in control and fructose (F) groups had free access to either plain drinking water or a solution of 20% D-fructose, combined with a standard diet for 8 wk. Rats in the fructose + activity (F + A) group performed voluntary physical activity with a running wheel. Blood pressure, serum glucose, lipids and natriuretic peptide levels were measured on the last day of the feeding period. In retroperitoneal adipose tissue, cGMP, hormone-sensitive lipase (HSL), perilipin-1, aquaglyceroporin levels, and adipocyte diameter were analyzed.

Results: Systolic blood pressure, glucose, and triacylglycerol were higher in the F groups compared to the control. The C-type natriuretic peptide was higher in the F group compared to the control. The cGMP level in retroperitoneal adipose tissue was higher in the F + A group than F group. Higher HSL and perilipin-1 levels were observed in the F + A group compared to the F and control groups. Adipocyte diameter was lower in the F + A group compared to the F group.

Conclusion: Regular physical exercise triggers lipolytic effects in adipose tissue through cGMP, HSL, and perilipin-1-mediated pathway in fructose-induced metabolic syndrome model in rats, preventing the increase in adipocyte diameter.

Purpose: The risk of inadequate micronutrient intake is a concern of low-climate impact diets. This study analyzes the prevalence of iron deficiency (ID) among adolescents with varying dietary climate impact, with special reference to different types and dietary sources of iron.

Methods: Data on dietary intake (n = 3099) and plasma ferritin (n = 1030) were from Riksmaten Adolescents 2016-2017 survey of Swedish girls and boys aged 11-18 years. Dietary climate impact was estimated with life cycle assessment data. Linear and logistic regression models assessed associations between dietary climate impact, intakes of iron and food groups, and ID.

Results: Higher total iron and heme, but not non-heme, iron intake, was linearly associated with higher dietary climate impact. Compared to girls, boys had higher climate impact and low prevalence of ID. Girls in the highest climate impact quartile had 56% lower odds of ID (OR 0.44, 95% CI 0.24-0.81) compared to the lowest quartile, whereas no association was found in boys. Lower intake of red meat and heme iron was associated with ID in girls, while higher intake of dairy was associated with ID in boys. Menstruating girls and adolescents born outside of Sweden were identified risk groups for ID.

Conclusions: Girls with a more climate-friendly diet and lower intake of red meat/heme iron may be at higher risk of ID compared to girls with higher dietary climate impact. These results highlight the importance of considering risk groups of ID, such as menstruating girls, in the transition to more plant-based diets with lower climate impact.

Purpose: This study aimed to evaluate whether maternal nutritional status, maternal age, mode of delivery, and the infant's sex influence the profiles of amino acids, energetic metabolites, sugars, and fatty acids and as well as the metabolic pathways in mature human milk human milk (HM).

Methods: This was a cross-sectional, prospective, and observational study. HM samples from normal weight (NW, n = 60), overweight (OW, n = 35), and obese (OB, n = 14) women were analyzed using a non-targeted GC-MS method to identify the metabolome. Data obtained were analyzed with Metaboanalyst software (v. 5.0) and SPSS (v.25.0).

Results: OB women HM contains a higher proportion of amino acids such as leucine, lysine, tyrosine, and aspartic acid, energy metabolites such as lactic and succinic acid, and sugars and derivatives such as fucose, rhamnose, and gluconic acid (p < 0.05) compared with normal weight women HM. HM from women > 25 years of age contains a lower proportion of lauric acid and a higher proportion of leucine and tyrosine (p < 0.05) than ≤ 25 years women HM. Also, HM intended for female infants has a higher leucine and gluconic acid content. The main altered metabolic pathways in OB women HM correspond to amino acids and energetic metabolism.

Conclusion: OB women HM provides more amino acids, energetic molecules, and sugars. Increased maternal weight, BMI, and body fat mass predispose to more leucine and aspartic acid in HM. Maternal age influences lauric acid, leucin, and tyrosine levels, while the infant's sex influences leucine and gluconic acid levels in HM. The impact of obese women's HM metabolome on the offspring's physiology needs to be explored.

Trial registration: R-2021-785-096.

Purpose: Nutrition, as a modifiable exposure, seems relevant to prevent depression. Several nutrients have been associated with a lower risk of depression in older adults, but longitudinal studies examining nutrient combinations are lacking. Therefore, we investigated the association between a posteriori nutrient patterns and the odds of depressive symptomatology (DS) in older adults over time.

Methods: The sample included participants from the French Three-City cohort, a prospective population-based study focusing on adults ≥ 65 years. Nutrient intakes were assessed using a 24-hour recall in 2001 and Principal Component Analysis based on 40 nutrients, was performed to identify nutrient patterns. DS was assessed at each visit (up to eight visits until 2018) and defined by a Center for Epidemiologic Studies-Depression score ≥ 16 and/or antidepressant medication. Random effect logistic regression was performed to examine the association between nutrient patterns and DS over time controlled for potential confounding variables.

Results: The study sample comprised 1,063 individuals (mean age 75.6y +/- 4.8) and 39.3% experienced DS at least once during a median follow-up of 11.5 years. Among 4 identified nutrient patterns, the first pattern characterised by high intake of magnesium, hydroxybenzaldehydes, stilbenes, dihydroflavonols, vitamin B6, lignans, tyrosols, folates, vitamins B3, B5, B1 and proanthocyanidins, was significantly associated with lower odds of DS over time (OR + 1 point = 0.85, 95% CI=[0.75;0.96]).

Conclusions: In this large sample of older adults, a mixed nutrient pattern was associated with lower odds of DS over time, offering valuable insights into nutrient potential role in mental health among older adults.

Purpose: To explore the associations of erythrocyte polyunsaturated fatty acids (PUFAs) with the risk of gestational diabetes mellitus (GDM) in pregnant women.

Methods: A total of 951 women in mid-pregnancy (20-28 weeks of gestation) were recruited during 2017-2018. Erythrocyte PUFAs were measured by gas chromatography and expressed as a percentage of total fatty acids. A 75 g oral glucose tolerance test was used for GDM diagnosis. Logistic regression and restricted cubic spline models were conducted.

Results: Among the 951 pregnant women, 180 were diagnosed with GDM (18.93%). For n-3 PUFAs, each standard deviation (SD) increase in α-linolenic acid (ALA) and docosapentaenoic acid (DPA) was associated with multivariable-adjusted odds ratios (ORs) of 0.789 (95% CI: 0.649, 0.961; P = 0.018) and 0.782 (95% CI: 0.638, 0.957; P = 0.017), respectively. However, the associations became marginally significant after post hoc false-discovery rate (FDR) correction (both P_FDR=0.065). A significant nonlinear association was observed for ALA and GDM risk (P_{- nonlinearity}=0.001). For n-6 PUFAs, γ-linolenic acid (GLA) was significantly associated with a 46.0% higher risk of GDM [OR (95%CI): 1.460 (1.195, 1.785), P_FDR=0.003) per SD increase], with a significant non-linear relationship (P_{- nonlinearity}=0.031). Arachidonic acid (AA) showed a borderline significant association with lower GDM risk after FDR correction [OR (95%CI): 0.736 (0.568, 0.953), P = 0.020, P_FDR=0.065].

Conclusion: Erythrocyte GLA was significantly associated with an increased risk of GDM in pregnant women. Furthermore, we observed for the first time significant non-linear associations of erythrocyte ALA and GLA with GDM risk.

Clinical trial registry number: NCT03023293, 2019-01-11.

Purpose: We aimed to examine the associations between plant-based dietary patterns, genetic risk, proteins, and lung cancer risk.

Methods: 189,541 participants of the UK Biobank were included. The Cox proportional hazards models and restricted cubic splines were performed to assess the associations between overall plant-based diet index(PDI), healthy PDI (hPDI), and unhealthy PDI (uPDI) and lung cancer risk. Polygenic risk score (PRS) was constructed to assess its joint effect with PDIs on lung cancer risk. Mediation analysis was conducted to examine which proteins mediated the associations of PDIs with incident lung cancer.

Results: Higher PDI was significantly correlated with reduced lung cancer risk(HR:0.90, 95% CI: 0.85-0.96, per SD). An elevated uPDI showed a significant correlation with an increased lung cancer risk(HR:1.08, 95% CI: 1.01-1.15, per SD). Individuals with a combination of high PRS and low PDI (HR: 2.09 [95% CI: 1.48-2.95]) or high uPDI (HR: 1.86 [95% CI: 1.36-2.53]) exhibited a notably higher lung cancer risk compared to those with low PRS and a high PDI or low uPDI. 42 proteins mediated the effect of PDI on lung cancer risk, and the association between uPDI with lung cancer was mediated by 117 proteins. The number of proteins displaying pathway enrichment within the cytokine-cytokine receptor interaction was the highest of the proteins mediated the effect of associations of PDI and uPDI with lung cancer risk.

Conclusion: A higher PDI correlated with reduced lung cancer risk, while a higher uPDI correlated with an elevated lung cancer risk. Low PDI jointing with high genetic risk increased lung cancer risk. Proteins enriched in cytokine-cytokine receptor interaction may mediate the association between plant-based dietary patterns with lung cancer risk.