Fish & shellfish immunology最新文献

{"title":"Mechanisms underlying differential utilization of carbohydrates from diverse structures and sources in Nile tilapia (Oreochromis niloticus): Insights from glycolipid metabolism, protein deposition and liver health","authors":"Meng-Ting Liu ,&nbsp;Chen-Chen Wu ,&nbsp;Si-Miao Pan ,&nbsp;Samwel Mchele Limbu ,&nbsp;Yu-Yang Liang ,&nbsp;Ying-Man Yu ,&nbsp;Fang Qiao ,&nbsp;Mei-Ling Zhang ,&nbsp;Zhen-Yu Du ,&nbsp;Yuan Luo","doi":"10.1016/j.fsi.2026.111187","DOIUrl":"10.1016/j.fsi.2026.111187","url":null,"abstract":"<div><div>The proper structure and source of dietary carbohydrates are vital for farmed fish health, yet their metabolic responses to varied carbohydrates are not clear. To explore fish metabolic responses to varied carbohydrates, Nile tilapia was fed with five different carbohydrate diets for eight weeks. The results showed that polysaccharides significantly enhanced the growth performance of fish (<em>P</em> &lt; 0.05). The fish fed with polysaccharides showed lower serum glucose, pyruvate and lactate, while their liver glucose uptake and catabolic capacity were significantly enhanced (<em>P</em> &lt; 0.05). Furthermore, the PDK4-PDHE1α axis, which linked glycolysis to the TCA cycle, exhibited a stronger response to polysaccharides, particularly in the corn starch group. The total lipid of the whole fish, as well as the TG in the liver and serum, were significantly elevated in the fish fed with wheat and tapioca starch (<em>P</em> &lt; 0.05). Additionally, the fish fed with wheat and tapioca starch exhibited a significant higher expression of PPARγ, DGAT and FAS (<em>P</em> &lt; 0.05). The serum levels of AST and ALT, as well as the liver MDA content, significantly decreased in the fish fed with polysaccharides (<em>P</em> &lt; 0.05). Meanwhile, the expression levels of pro-inflammatory (TNFα and IL12) and apoptosis (caspase8 and caspase9) -related genes in the liver were significantly downregulated, while the antioxidant capacity markedly increased (<em>P</em> &lt; 0.05). The total protein of the whole fish, muscle protein content, and muscle fiber diameter of fish fed polysaccharides were significantly increased, particularly in the corn starch group (<em>P</em> &lt; 0.05). Furthermore, the fish fed with polysaccharides exhibited stronger mTOR signaling response and higher protein synthesis capacity in the muscle, particularly in the corn starch group (<em>P</em> &lt; 0.05). This study demonstrated that the PDK4-PDHE1α axis and mTOR exhibit a stronger response to polysaccharides, boosting carbohydrate breakdown and protein synthesis for growth. PPARγ responds more robustly to wheat and tapioca starch, facilitating the esterification of free fatty acids into TG, which reduced lipid toxicity and maintained liver health.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111187"},"PeriodicalIF":3.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146124258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA-seq reveals cellular dynamics and emergency hematopoiesis in banana shrimp Penaeus merguiensis hemocytes upon Yellow Head Virus infection","authors":"Keiichiro Koiwai ,&nbsp;Phattarunda Jaree ,&nbsp;Kanjanaporn Nanthajak ,&nbsp;Masachika Harada ,&nbsp;Hiroki Kusaka ,&nbsp;Ammara Sinprasertporn ,&nbsp;Hajime Yuasa ,&nbsp;Kayo Konishi ,&nbsp;Reiko Nozaki ,&nbsp;Hidehiro Kondo ,&nbsp;Jumroensri Thawonsuwan ,&nbsp;Kunlaya Somboonwiwat ,&nbsp;Ikuo Hirono","doi":"10.1016/j.fsi.2026.111178","DOIUrl":"10.1016/j.fsi.2026.111178","url":null,"abstract":"<div><div>As global aquaculture of penaeid shrimp expands, the banana shrimp (<em>Penaeus merguiensis</em>) has gained attention as a promising sustainable alternative to the non-native <em>P. vannamei</em>. However, its cellular immune response to major pathogens like yellow head virus (YHV) remains poorly understood, limiting effective disease management. In this study, we established a comprehensive reference transcriptome for <em>P. merguiensis</em> by integrating data from 13 major tissues and conducted single-cell RNA sequencing (scRNA-seq) on hemocytes under normal and YHV-infected conditions. We identified eight distinct hemocyte clusters (cluster 0–7) and demonstrated that YHV infection triggers a massive turnover of the hemocyte landscape. Specifically, mature immune-active clusters expressing <em>anti-lipopolysaccharide factor</em>s (<em>ALF</em>s) and <em>lysozyme</em>s were significantly depleted, likely due to virus-induced apoptosis. Conversely, a marked expansion of an immature hemocyte progenitor cluster (cluster 4) was observed, indicating a host-driven emergency hematopoiesis response. Our analysis further revealed that global transcriptional changes are heavily biased by these composition shifts: 30.7% of the total 114 globally regulated genes were identified as cluster-specific markers. These findings provide a high-resolution model of crustacean immune dynamics and highlight the necessity of single-cell analysis for accurately deciphering host-pathogen interactions.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111178"},"PeriodicalIF":3.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indole-3-propionic acid enhances intestinal health and protects against Aeromonas hydrophila infection in high-fat-diet fed Nile tilapia (Oreochromis niloticus)","authors":"Fei-Fei Ding ,&nbsp;Shi-Qi Deng ,&nbsp;Yu-Xin Fan ,&nbsp;Shi-Yan Qiao ,&nbsp;Gang Wang","doi":"10.1016/j.fsi.2026.111180","DOIUrl":"10.1016/j.fsi.2026.111180","url":null,"abstract":"<div><div>High fat diet (HFD) is widely utilized in aquaculture to promote growth and feed protein retention, but overconsumption of fat will induce systemic metabolic disorders of fish accompanied by impaired intestinal health and lower pathogen resistance. Indole-3-propionic acid (IPA) has been widely reported to enhance intestinal health in mammals. Our previous study has proven that IPA alleviated lipid metabolism disorder and lipotoxic liver injury in Nile tilapia (<em>Oreochromis niloticus</em>). However, it remains unclear whether IPA can sustain fish intestinal health. In this study, 540 Nile tilapia (2.53 ± 0.02 g) were allotted in 6 groups with 3 tanks each and fed with control diet or high-fat diet supplemented with different levels of IPA (0, 20, 40, 80, 160 mg/kg) respectively. After 8 weeks, it was observed that 80 mg/kg IPA proved the most beneficial for decreasing intestinal myeloperoxidase (MPO) enzyme activity and improving the survival rate of Nile tilapia infected with <em>Aeromonas hydrophila</em>. Dietary IPA restored intestinal barrier integrity impaired by HFD and increased the genes expression of tight junctional proteins (tjps), such as zona occludens 1 (<em>zo-1</em>) and <em>occludin</em>. The results showed that dietary IPA effectively mitigated HFD caused intestinal oxidative stress, manifesting as lower levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and higher activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Dietary IPA promoted the gene and protein expression of aryl hydrocarbon receptor (Ahr) in intestine. Further investigation suggested that IPA could activate Ahr and affect nuclear factor-E2-related factor 2 (NRF2) signaling to reduce oxidative stress. Moreover, IPA might suppress enteritis by inhibiting the Ahr-mediated nuclear factor kappa light-chain-enhancer of activated B (NF-κB) signaling pathway. These findings indicated that IPA has the potential to improve intestinal health and pathogen resistance in HFD-fed fish, offering crucial insights for the application of innovative additives in aquatic feeds.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111180"},"PeriodicalIF":3.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two CC motif chemokine 20-like genes differentially regulate leukocyte migration and survival via CC chemokine receptor 6 in ayu (Plecoglossus altivelis)","authors":"Jia-Feng Cao ,&nbsp;Yan Zhou ,&nbsp;Li-Jun Duan ,&nbsp;Shi-Li Shang ,&nbsp;Qian-Jin Zhou ,&nbsp;Jiong Chen","doi":"10.1016/j.fsi.2026.111176","DOIUrl":"10.1016/j.fsi.2026.111176","url":null,"abstract":"<div><div>Chemokine ligand 20 (CCL20) is a key mediator of inflammatory and homeostatic responses in mammal, acting through its receptor CC chemokine receptor 6 (CCR6). However, its functional roles in teleost fish remain poorly defined. In this study, we identified two <em>CCL20</em>-like genes (<em>PaCCL20l1</em> and <em>PaCCL20l2</em>) from ayu (<em>Plecoglossus altivelis</em>). Phylogenetic analysis confirmed their classification within the CCL20 subgroup. Tissue distribution analysis revealed ubiquitous expression of both genes, which were significantly upregulated in multiple tissues (liver, spleen, head kidney, gill, skin, and intestine) following infection with <em>Vibrio anguillarum</em>, albeit with distinct expression profiles. We produced the recombinant mature peptide of PaCCL20ls (rPaCCL20l1 and rPaCCL20l2) and generated specific antibodies. Functional assays demonstrated that both rPaCCL20l1 and rPaCCL20l2 exhibit chemotactic, and anti-apoptosis activities, but with differing cell specificities. rPaCCL20l1 attracted lymphocytes and neutrophils and inhibited lymphocyte apoptosis. In contrast, rPaCCL20l2 attracted monocytes/macrophages (MO/MΦ), lymphocytes, and neutrophils, and suppressed apoptosis in both MO/MΦ and lymphocytes. Crucially, neutralizing PaCCR6 completely abolished all effects of both rPaCCL20l1 and rPaCCL20l2. In conclusion, our findings demonstrate that <em>PaCCL20l1</em> and <em>PaCCL20l2</em> play pivotal but distinct roles in ayu immunity by modulating leukocyte recruitment and survival via PaCCR6 pathway.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111176"},"PeriodicalIF":3.9,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146104451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and expression profile of serotonin receptors in the developmental stages and hemocytes of the Pacific oyster (Crassostrea gigas)","authors":"Minhui Xu ,&nbsp;Miren Dong ,&nbsp;Xiaolong Gao ,&nbsp;Wei Wu ,&nbsp;Lingling Wang ,&nbsp;Chunlin Wang","doi":"10.1016/j.fsi.2026.111148","DOIUrl":"10.1016/j.fsi.2026.111148","url":null,"abstract":"<div><div>Serotonin (5-hydroxytryptamine, 5-HT), a pleiotropic biogenic monoamine, modulates immune response through interactions with its distinct receptors (5-HTRs). In this study, thirteen 5-HTRs were identified in the Pacific oyster (<em>Crassostrea gigas</em>) to elucidate their functions in immune response to <em>Vibrio</em> stimulation. Except for the unique ligand-gated cation channels (<em>Cg</em>5-HTR3A-like), the other twelve 5-HTRs (Two <em>Cg</em>5-HTR, <em>Cg</em>5-HTR-like, <em>Cg</em>5-HTR1, <em>Cg</em>5-HTR1A-like, <em>Cg</em>5-HTR1B, <em>Cg</em>5-HTR1B-like, <em>Cg</em>5-HTR2A-like, <em>Cg</em>5-HTR2B, <em>Cg</em>5-HTR4, <em>Cg</em>5-HTR5, and <em>Cg</em>5-HTR6) all belong to G protein-coupled receptor (GPCR) superfamily. Among those twelve 5-HTRs, most are non-canonical members lacking complete seven-transmembrane (7TM) domains; only <em>Cg</em>5-HTR2B and <em>Cg</em>5-HTR6 retain the canonical GPCR architecture. Ten conserved motifs were identified in oyster 5-HTRs, with motif 2 being universal across all receptors and motif 10 unique to <em>Cg</em>5-HTR3A-like, indicating subtype-specific divergence. Molecular docking revealed significant differences in binding affinity and residue interaction among <em>Cg</em>5-HTRs, with <em>Cg</em>5-HTR6 exhibiting the highest binding affinity for 5-HT. Analysis across developmental stages revealed that <em>Cg</em>5-HTR1A-like transcripts were enriched in early gastrula, gastrula and trochophore, <em>Cg</em>5-HTR4, <em>Cg</em>5-HTR1B, and <em>Cg</em>5-HTR-like enriched specifically in the D-shape stage, while <em>Cg</em>5-HTR2B and <em>Cg</em>5-HTR1 transcripts enriched during the pediveliger, spat, and juvenile stages. Furthermore, <em>Cg</em>5-HTR-like and <em>Cg</em>5-HTR1 were primarily expressed in granulocytes, <em>Cg</em>5-HTR1A-like and <em>Cg</em>5-HTR2B in agranulocytes, and <em>Cg</em>5-HTR6 and <em>Cg</em>5-HTR1B in semi-granulocytes. Crucially, following secondary <em>Vibrio splendidus</em> stimulation, the expression levels of <em>Cg</em>5-HTR-like, <em>Cg</em>5-HTR1, <em>Cg</em>5-HTR1A-like, <em>Cg</em>5-HTR1B and <em>Cg</em>5-HTR2B mRNA in hemocytes upregulated significantly, indicating their potential role in immune priming. These findings suggested the potential mechanisms of 5-HTRs in regulating the immune response of hemocytes in <em>C. gigas</em>, which offered insights into the evolutionary conservation and immunoregulation divergence of these receptors in invertebrates.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"172 ","pages":"Article 111148"},"PeriodicalIF":3.9,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and efficacy of autogenous multivalent vaccine to prevent Motile Aeromonas Septicemia (MAS) and Bacillary Necrosis of Pangasius (BNP) in striped catfish (Pangasianodon hypophthalmus)","authors":"Le Minh Khoi ,&nbsp;Thea Aldiano ,&nbsp;Nguyen Bao Trung ,&nbsp;Tran Thi Tuyet Hoa ,&nbsp;Tu Thanh Dung","doi":"10.1016/j.fsi.2026.111177","DOIUrl":"10.1016/j.fsi.2026.111177","url":null,"abstract":"<div><div>Vaccines are central to disease prevention in farmed fish and help reduce antimicrobial use in aquaculture. Autogenous vaccines represent a practical solution for managing pathogens with high genetic variability, particularly in urgent outbreaks. In this study, 28 bacterial strains were isolated from striped catfish (<em>P. hypophthalmus</em>) suffering from Motile Aeromonas Septicaemia (MAS) and Bacillary Necrosis of Pangasius (BNP). Strains were identified using specific primers, 16S rRNA gene sequencing, and DNA fingerprinting, followed by virulence gene screening and pathogenicity testing. Two strains of <em>Aeromonas</em> VH31, VH74, and two strains of <em>Edwardsiella ictaluri</em> VH83, VH116 were selected to formulate injectable autogenous oil-emulsion vaccines. Experimental trials included four groups of bivalent vaccines (VC-VH31/83, VC-VH31/116, VC-VH74/83, VC-VH74/116), one multivalent vaccine (VC-VHMix), and a PBS control, evaluated over 60 days. The multivalent vaccine conferred the highest relative percent survival (91.7%), while VC-VH31/83 and VC-VH31/116 showed moderate protection (66.7% and 70.8%, respectively). In contrast, VC-VH74/83 and VC-VH74/116 yielded minimal protection (12.5%). Serum IgM antibody analysis showed that the fish had an immune response to <em>Aeromonas</em> and <em>Edwardsiella</em> antigens after 15–30 days of vaccination in the vaccine injection treatments. The concentration of total WBCs, lymphocytes, and granulocytes in the blood of the fish increased and peaked after 45 days. Overall growth performance in the most effective vaccine groups (VC-VHMix, VC-VH31/83, VC-VH31/116) was not significantly affected compared to the control. These results demonstrate that autogenous vaccines are a promising approach to control dual infections of MAS and BNP in striped catfish and highlight their potential role in sustainable aquaculture disease management.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111177"},"PeriodicalIF":3.9,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146104477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of intraperitoneal miconazole nitrate against Saprolegnia parasitica in Labeo rohita under feeding refusal conditions","authors":"Mukta Singh ,&nbsp;Ratan Kumar Saha ,&nbsp;Himadri Saha","doi":"10.1016/j.fsi.2026.111167","DOIUrl":"10.1016/j.fsi.2026.111167","url":null,"abstract":"<div><div>Fungal infections caused by <em>Saprolegnia parasitica</em> are a major concern in freshwater aquaculture, particularly during advanced stages of disease when affected fish often exhibit feeding refusal, rendering oral medication ineffective. This study investigates the therapeutic potential of intraperitoneally administered miconazole nitrate (MCZ) in <em>Labeo rohita</em> fingerlings under such critical conditions. The 96-h LD₅₀ of MCZ was determined to be 0.20 ± 0.13 mg kg⁻¹ body weight (BW). Three sub-lethal doses (0.01, 0.02, and 0.04 mg MCZ kg⁻¹ BW) were evaluated alongside a DMSO control over eight time points (0–240 h), analyzing haematological and immuno-biochemical parameters including erythrocyte and leukocyte counts, hemoglobin, hematocrit, Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), glucose, plasma ion concentrations, lysozyme activity, total protein, and respiratory burst activity. A dose-dependent physiological response was observed, with lower doses (especially 0.01 mg MCZ kg⁻¹ BW) exhibiting minimal adverse effects while enhancing immune indicators such as lysozyme and respiratory burst activity. Challenge trials with <em>S. parasitica</em> zoospores further confirmed higher survival and recovery in the low-dose group. These findings highlight the efficacy of injectable MCZ as a vital therapeutic alternative during feeding suppression, offering a targeted strategy for managing saprolegniasis in aquaculture.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111167"},"PeriodicalIF":3.9,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel stomach-predominant C-type lectin (LvnCTL) triggers immune response against Vibrio parahaemolyticus and WSSV in shrimp Litopenaeus vannamei","authors":"Shengwen Niu ,&nbsp;Ning Fu ,&nbsp;Yuyu Wang ,&nbsp;Mengxin Xing ,&nbsp;Meiling An ,&nbsp;Hongli Xu ,&nbsp;Bang Xiao ,&nbsp;Weiyan Song ,&nbsp;Yongqing Zeng ,&nbsp;Orlando Borras-Hidalgo ,&nbsp;Hui Wang","doi":"10.1016/j.fsi.2026.111158","DOIUrl":"10.1016/j.fsi.2026.111158","url":null,"abstract":"<div><div>C-type lectins (CTLs) are members of pattern recognition receptors (PRRs), which can bind to carbohydrates on the surface of pathogens and take part in the immune response of shrimp. In this study, we identified and characterized a novel stomach-predominant C-type lectin (named LvnCTL) in <em>Litopenaeus vannamei</em> (<em>L. vannamei</em>). LvnCTL consists of a signal peptide at the N-terminal and a typical C-type lectin-like domain (CTLD) at the C-terminal. Phylogenetic analysis revealed that LvnCTL clusters into a distinct clade more closely related to vertebrate CTLs than to invertebrate CTLs. LvnCTL was highly expressed in the stomach, and its expression was significantly induced by immune stimuli and pathogen infections. Functional assays showed that silencing of LvnCTL enhanced the replication of <em>Vibrio parahaemolyticus</em> and white spot syndrome virus (WSSV), thereby increasing shrimp susceptibility. In contrast, recombinant LvnCTL effectively agglutinated both the Gram-negative bacterium <em>V. parahaemolyticus</em> and the Gram-positive bacterium <em>Staphylococcus aureus</em> in a Ca²⁺-dependent manner. Furthermore, LvnCTL silencing markedly altered the expression of immune-related genes, including antimicrobial peptides, cytokines, and prophenoloxidase (proPO) genes. Taken together, these findings suggest that LvnCTL plays a crucial role in the shrimp immune response to inhibit pathogen invasion.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111158"},"PeriodicalIF":3.9,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146096966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on hemolymph sampling methods: Monthly variations in hemocyte characteristics of farmed oyster Crassostrea corteziensis (Bivalvia: ostreidae)","authors":"Miguel Ángel Hurtado–Oliva ,&nbsp;Elena Palacios–Mechetnov","doi":"10.1016/j.fsi.2026.111153","DOIUrl":"10.1016/j.fsi.2026.111153","url":null,"abstract":"<div><div>Immune bivalve mollusk response is closely related to environmental conditions (i.e. seawater temperature and salinity, availability and food quality); including routine handling that occurs during sea farming, such as manipulation for size sorting and cleaning epibionts from shells. Storage (hypoxia, dry conditions, etc.), transportation, and acclimatization to laboratory conditions of wild or reared organisms are also known to exert significant changes in bivalve mollusk immune cell responses. Therefore, the aim of the present study is to assess the effect of hemolymph withdrawal handled in two different conditions (field vs. laboratory) in monthly variations of both morphological and functional hemocyte characteristics in oyster <em>C. corteziensis</em>. Hemolymph samples were extracted from oysters immediately sampled at the farming site (field), and from oysters stored in dry-cold conditions and terrestrially transported during 2 h to the laboratory facilities. Total (THC) and differential (DHC) hemocyte counts, hemocyte mortality (HM), and anion superoxide production (ASO/hemocyte) were assessed in hemolymph samples obtained from oysters in both handled conditions (field vs. laboratory). Sea surface temperature (SST), salinity, pH, seston (total, inorganic, and organic particulate matter), and chlorophyll-a (Chl-a) were monthly assessed in seawater samples. THC, DHC, HM, and ASO/hemocyte production were not different between hemolymph samples withdrawal from oysters handled in both conditions. All immune parameters assessed were significantly different between sampling dates, but most were not significantly correlated with physicochemical seawater parameters assessed, except THC and hyalinocyte count (HC), which were positively correlated with phytoplankton availability (i.e. Chl-a) that increased during winter, and granulocytes that increased with SST rise but negatively correlated with OCI, along with seston availability. HM decreased with THC and HC increase, suggesting a link with hemocyte proliferation but without compromising immune response. Hemocyte oxidative capacity (i.e. ASO/hemocyte) increased significantly with oyster condition, which coincided with the increase of phytoplankton availability (i.e. Chl-a). Although increasing energy allocation improves oyster physiological condition –including immune response– circulating hemocyte increased and their respective oxidative capacity were more likely to be due to a response to availability and consumption on certain toxin-producing phytoplankton species (dinoflagellates). Hemolymph withdrawal from oysters directly in field could be advantageous, mainly when no other tissues than immune cells and plasma are required to be analyzed, avoiding whole oyster transportation.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"171 ","pages":"Article 111153"},"PeriodicalIF":3.9,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146092607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Identification and functional characterization of cGAS and cGAS-like in snakehead Channa argus” [Fish Shellfish Immunol. 165 (2025) 110566]","authors":"Hong Qing Zhang ,&nbsp;Long Wei Cai ,&nbsp;Yu Hang Deng ,&nbsp;Meng Xin Yan ,&nbsp;Peng Fei Mu ,&nbsp;Bo Li ,&nbsp;Lan Hao Liu ,&nbsp;P. Nie","doi":"10.1016/j.fsi.2026.111149","DOIUrl":"10.1016/j.fsi.2026.111149","url":null,"abstract":"","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"170 ","pages":"Article 111149"},"PeriodicalIF":3.9,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146085025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}