O. Gorenskaya, V. Navrotskaya, N. Volkova, N. S. Filiponenko
Aim. To compare reproductive indices and stress resistance of Drosophila at outbreeding and inbreeding. Methods. Drosophila melanogaster congenic strains with incomplete development of the radial wing vein – radius incompletus – were used: the laboratory one and the strain, in which the mutation was placed into the genetic background of wild type strain, which originates from the natural population from radiation contaminated territory. Before the experiment strains have passed 65 generations of inbreeding. Viability (number of individuals, pupa stage mortality), dominant lethal mutations frequency and life span of imago at starvation were analysed. Results. After inbreeding, there was a decrease in the frequency of dominant lethal mutations and an increase in viability of the strain, which originates from the natural population, and a decrease of mortality at the pupal stage in both strains. Decreased life span of imago at starvation has been shown only for the inbred strain, which originates from the natural population. Conclusions. Inbreeding for 65 generations has no significant negative effect on reproductive indices; reduction of stress resistance during inbreeding has been shown only for the strain, which originates from the radiation contaminated territory. Keywords: Drosophila, viability, dominant lethal mutations, life span of imago at starvation, inbreeding.
{"title":"Inbreeding effects in Drosophila congenic strains: the influence of genetic background of different origin","authors":"O. Gorenskaya, V. Navrotskaya, N. Volkova, N. S. Filiponenko","doi":"10.7124/feeo.v28.1369","DOIUrl":"https://doi.org/10.7124/feeo.v28.1369","url":null,"abstract":"Aim. To compare reproductive indices and stress resistance of Drosophila at outbreeding and inbreeding. Methods. Drosophila melanogaster congenic strains with incomplete development of the radial wing vein – radius incompletus – were used: the laboratory one and the strain, in which the mutation was placed into the genetic background of wild type strain, which originates from the natural population from radiation contaminated territory. Before the experiment strains have passed 65 generations of inbreeding. Viability (number of individuals, pupa stage mortality), dominant lethal mutations frequency and life span of imago at starvation were analysed. Results. After inbreeding, there was a decrease in the frequency of dominant lethal mutations and an increase in viability of the strain, which originates from the natural population, and a decrease of mortality at the pupal stage in both strains. Decreased life span of imago at starvation has been shown only for the inbred strain, which originates from the natural population. Conclusions. Inbreeding for 65 generations has no significant negative effect on reproductive indices; reduction of stress resistance during inbreeding has been shown only for the strain, which originates from the radiation contaminated territory. \u0000Keywords: Drosophila, viability, dominant lethal mutations, life span of imago at starvation, inbreeding.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88056436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The aim of research was to analyze the activation of Triticum aestivum L. non-specific resistance by the effect of ferulic acid and NO on H2O2 content against fungal pathogen from environment in field trials. Methods. Content of endogenous H2O2 was measured in elicitor treated and infected wheat plants (cv. Oberig myronivskij and Svitanok myronivskij) during different ontogenesis phases. The extent of disease development, morphometric parameters and yield structure were analyzed. Results. The data obtained suggest that different levels of endogenous hydrogen peroxide were induced in wheat leaves by treatment. The growth and yield were stimulated. The infection damage decreased. Conclusions. The role of endogenous hydrogen peroxide is crucial for wheat defense during all vegetation period. The elicitor and donor NO induced effective defense responses and resistance in winter wheat against Erysiphe graminis. Keywords: Triticum aestivum L., ferulic acid, NO, induced resistance, powdery mildew.
{"title":"The activation of wheat resistance against powdery mildew by combination of biotic elicitor and NO donor","authors":"I. Zhuk, Yu. V. Shylina, A. P. Dmytriev","doi":"10.7124/feeo.v28.1379","DOIUrl":"https://doi.org/10.7124/feeo.v28.1379","url":null,"abstract":"Aim. The aim of research was to analyze the activation of Triticum aestivum L. non-specific resistance by the effect of ferulic acid and NO on H2O2 content against fungal pathogen from environment in field trials. Methods. Content of endogenous H2O2 was measured in elicitor treated and infected wheat plants (cv. Oberig myronivskij and Svitanok myronivskij) during different ontogenesis phases. The extent of disease development, morphometric parameters and yield structure were analyzed. Results. The data obtained suggest that different levels of endogenous hydrogen peroxide were induced in wheat leaves by treatment. The growth and yield were stimulated. The infection damage decreased. Conclusions. The role of endogenous hydrogen peroxide is crucial for wheat defense during all vegetation period. The elicitor and donor NO induced effective defense responses and resistance in winter wheat against Erysiphe graminis. \u0000Keywords: Triticum aestivum L., ferulic acid, NO, induced resistance, powdery mildew.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85173600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The aim of the study was to identify and systematize methodological problems in the diagnosis of anthropogenic transformation of forest ecosystem. Methods. Theoretical methods of system-structural, retrospective analysis, interdisciplinary, ecosystem approaches, special methods of forestry, synecology, ornithology and mycology. Results. Changes in functional indicators and parameters of communities were recorded depending on the genesis of the anthropogenic factor, pattern, intensity and time-scale of its impact and reproducibility of ecosystems. These changes were established in the presence of close correlation between the most sensitive structural and functional components (grass layer, leaf-litter, surface of soil, stand) and preserving the integrity of consorted links. Conclusions. A conceptual model for diagnostic human transformation of forest ecosystems for various functional according to the state and development of consorted links of producers, consumers, reducers (for example, plants, fungi, birds) through qualitative and quantitative parameters of relevant diversity structures and principles of its application was proposed. Keywords: ecosystem, communities, anthropogenic factor, transformation, biodiversity, consorted links.
{"title":"Problem issues of methodology of synecological diagnostics of anthropogenic transformation of forest ecosystems","authors":"O. Blinkova","doi":"10.7124/feeo.v28.1392","DOIUrl":"https://doi.org/10.7124/feeo.v28.1392","url":null,"abstract":"Aim. The aim of the study was to identify and systematize methodological problems in the diagnosis of anthropogenic transformation of forest ecosystem. Methods. Theoretical methods of system-structural, retrospective analysis, interdisciplinary, ecosystem approaches, special methods of forestry, synecology, ornithology and mycology. Results. Changes in functional indicators and parameters of communities were recorded depending on the genesis of the anthropogenic factor, pattern, intensity and time-scale of its impact and reproducibility of ecosystems. These changes were established in the presence of close correlation between the most sensitive structural and functional components (grass layer, leaf-litter, surface of soil, stand) and preserving the integrity of consorted links. Conclusions. A conceptual model for diagnostic human transformation of forest ecosystems for various functional according to the state and development of consorted links of producers, consumers, reducers (for example, plants, fungi, birds) through qualitative and quantitative parameters of relevant diversity structures and principles of its application was proposed. \u0000Keywords: ecosystem, communities, anthropogenic factor, transformation, biodiversity, consorted links.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86405016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Aim of the study was to microscopically investigate the interaction of Acholeplasma laidlawii var. granulum 118 with the target plant Medicago sativa, as well as to determine the role of Rhizobium meliloti strains with natural and defective polysaccharides in the plant’s resistance occurrence. Methods. Cultivation of plants and their inoculation with bacteria was performed under conditions of microvegetation experiment. The study of the ultrastructure of alfalfa roots and nodules was carried out with both light and electron microscopy. Results. The rhizobial mutant strain, defective in the synthesis of lipopolysaccharides, more often formed atypical nodules on M. sativa, which aged faster. In the variant with double inoculation of alfalfa with rhizobia together with acholeplasma changes in the morphology of the lateral roots of plants, as well as deformation of the nodules were observed. Conclusions. Results of this study indicate not only the ability of phytoplasmas to penetrate the root system and migrate to plant aboveground organs, but also demonstrate the possibility of their presence in the nodules formed by rhizobia. Keywords: Mollicutes, acholeplasma, rhizobia, mutant, lipopolysaccharides.
{"title":"Microscopic studies of the phytoplasma infection effect on the symbiotic system Medicago sativa – Rhizobium meliloti in simulated conditions","authors":"K. S. Korobkova, T. V. Zatovska, M. Kharchuk","doi":"10.7124/feeo.v28.1370","DOIUrl":"https://doi.org/10.7124/feeo.v28.1370","url":null,"abstract":"Aim. Aim of the study was to microscopically investigate the interaction of Acholeplasma laidlawii var. granulum 118 with the target plant Medicago sativa, as well as to determine the role of Rhizobium meliloti strains with natural and defective polysaccharides in the plant’s resistance occurrence. Methods. Cultivation of plants and their inoculation with bacteria was performed under conditions of microvegetation experiment. The study of the ultrastructure of alfalfa roots and nodules was carried out with both light and electron microscopy. Results. The rhizobial mutant strain, defective in the synthesis of lipopolysaccharides, more often formed atypical nodules on M. sativa, which aged faster. In the variant with double inoculation of alfalfa with rhizobia together with acholeplasma changes in the morphology of the lateral roots of plants, as well as deformation of the nodules were observed. Conclusions. Results of this study indicate not only the ability of phytoplasmas to penetrate the root system and migrate to plant aboveground organs, but also demonstrate the possibility of their presence in the nodules formed by rhizobia. \u0000Keywords: Mollicutes, acholeplasma, rhizobia, mutant, lipopolysaccharides.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78639154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Buhaiov, V. Horenskyy, V. S. Mamalyga, I. V. Smulskaya
Aim. Evaluation of the source material on a set of economically valuable features and the creation with its participation of a synthetic variety of alfalfa. Methods. Field, laboratory, statistical. Results. The results of research and creation of a synthetic variety of sown alfalfa Rodena at the Institute of Forage and Agriculture of Podillya NAAS are presented. Entered into the State Register of Plant Varieties Suitable for Distribution in Ukraine (hereinafter - the Register of Plant Varieties of Ukraine). When evaluating the forage and seed productivity in the experimental fields of the institute, the advantage of the Roden variety to the Sinyukha standard variety was established. Extensive testing of the variety's suitability for propagation (PSP) in 9 research points by the Ukrainian Institute of Plant Variety Examination confirmed higher indicators of fodder and seed productivity of this variety in comparison with the conditional standard. Conclusions. The use of clonal genotypes, which, along with a set of economically valuable traits are distinguished by high overall combining ability, allows you to effectively create high-yielding varieties of synthetic alfalfa. Created in this way, the synthetic variety of alfalfa Rodena is recommended for use in pure and mixed crops with other perennial grasses for the production of high quality feed in the soil-climatic zone of the Steppe of Ukraine. Keywords: selection, alfalfa sowing, clone, synthetic variety, winter hardiness, dry matter, seeds.
{"title":"New generation synthetic variety of lucerne (Medicago sativa L.)","authors":"V. Buhaiov, V. Horenskyy, V. S. Mamalyga, I. V. Smulskaya","doi":"10.7124/feeo.v29.1400","DOIUrl":"https://doi.org/10.7124/feeo.v29.1400","url":null,"abstract":"Aim. Evaluation of the source material on a set of economically valuable features and the creation with its participation of a synthetic variety of alfalfa. Methods. Field, laboratory, statistical. Results. The results of research and creation of a synthetic variety of sown alfalfa Rodena at the Institute of Forage and Agriculture of Podillya NAAS are presented. Entered into the State Register of Plant Varieties Suitable for Distribution in Ukraine (hereinafter - the Register of Plant Varieties of Ukraine). When evaluating the forage and seed productivity in the experimental fields of the institute, the advantage of the Roden variety to the Sinyukha standard variety was established. Extensive testing of the variety's suitability for propagation (PSP) in 9 research points by the Ukrainian Institute of Plant Variety Examination confirmed higher indicators of fodder and seed productivity of this variety in comparison with the conditional standard. Conclusions. The use of clonal genotypes, which, along with a set of economically valuable traits are distinguished by high overall combining ability, allows you to effectively create high-yielding varieties of synthetic alfalfa. Created in this way, the synthetic variety of alfalfa Rodena is recommended for use in pure and mixed crops with other perennial grasses for the production of high quality feed in the soil-climatic zone of the Steppe of Ukraine. \u0000Keywords: selection, alfalfa sowing, clone, synthetic variety, winter hardiness, dry matter, seeds.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90937691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Chorna, H. Makukh, D. Zastavna, Ya.Yu. Zaganyach, O. Kolodiy, O. Kovtun
Aim. Despite numerous scientific studies of possible causes of miscarriage, their etiology remains unclear in approximately 50% of cases. Investigate the prevalence of thrombophilia associated gene polymorphism FGB 455G/A, FII 20210 G/A, FV 1691G/A, ITGA2 807C/T, PAI-1 5G/4G and MTHFR 677C/T in women with sporadic and recurrent miscarriages. Methods. Group I included 35 women with sporadic miscarriage (SM), group II consisted of 57 women with recurrent miscarriage (RM) and 55 women of control group. Genetic testing was performed by PCR-RFLP. Results. In group I of women with SM the 455GA genotype of the FGB gene was more common and its presence in the genotype increases the risk of SM by 4 times and the presence of the 455A allele by 2 times. The Leiden mutation increases the risk of SM by 5 times. In II group of women with RM, the frequency of the 455 AA genotype of the FGB gene was more prevalent and the risk of RM was increased 2.5 times. It is shown that the risk of RM increases 4 times in the presence of the Leiden mutation. The 4G allele of the PAI-1 5G/4G polymorphism leads to a 2-fold increase in the risk of RM, and the presence of the 677TT genotype of the MTHFR gene increases the risk of RM by 3 times. Conclusions. Genetic factors of inherited thrombophilia alleles 455A of the FGB gene, 1691A of the FV gene, 4G of the PAI-1 gene and 677T of the MTHFR gene are alleles of significant risk of reproductive losses both sporadic and, to a greater extent, recurrent. Keywords: sporadic miscarriage, recurrent.miscarriage, inherited thrombophilia, genetic polymorphism.
{"title":"Association of inherited thrombophilia gene polymorphism with sporadic and recurrent miscarriages","authors":"L. Chorna, H. Makukh, D. Zastavna, Ya.Yu. Zaganyach, O. Kolodiy, O. Kovtun","doi":"10.7124/feeo.v29.1426","DOIUrl":"https://doi.org/10.7124/feeo.v29.1426","url":null,"abstract":"Aim. Despite numerous scientific studies of possible causes of miscarriage, their etiology remains unclear in approximately 50% of cases. Investigate the prevalence of thrombophilia associated gene polymorphism FGB 455G/A, FII 20210 G/A, FV 1691G/A, ITGA2 807C/T, PAI-1 5G/4G and MTHFR 677C/T in women with sporadic and recurrent miscarriages. Methods. Group I included 35 women with sporadic miscarriage (SM), group II consisted of 57 women with recurrent miscarriage (RM) and 55 women of control group. Genetic testing was performed by PCR-RFLP. Results. In group I of women with SM the 455GA genotype of the FGB gene was more common and its presence in the genotype increases the risk of SM by 4 times and the presence of the 455A allele by 2 times. The Leiden mutation increases the risk of SM by 5 times. In II group of women with RM, the frequency of the 455 AA genotype of the FGB gene was more prevalent and the risk of RM was increased 2.5 times. It is shown that the risk of RM increases 4 times in the presence of the Leiden mutation. The 4G allele of the PAI-1 5G/4G polymorphism leads to a 2-fold increase in the risk of RM, and the presence of the 677TT genotype of the MTHFR gene increases the risk of RM by 3 times. Conclusions. Genetic factors of inherited thrombophilia alleles 455A of the FGB gene, 1691A of the FV gene, 4G of the PAI-1 gene and 677T of the MTHFR gene are alleles of significant risk of reproductive losses both sporadic and, to a greater extent, recurrent. \u0000Keywords: sporadic miscarriage, recurrent.miscarriage, inherited thrombophilia, genetic polymorphism.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87953186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To investigate the functionality of the transgene in the seed generation (T2) of genetically modified winter wheat plants (Triticum aestivum L.) in terms of productivity in conditions of normal and insufficient water supply. Methods. Determination of yield structure and protein content. Results. The indicators of productivity of control and T2 biotechnological plants under normal growing conditions and water deficit are analyzed. The quantitative composition of protein in the conditions before and after osmotic stress and in the period of rehydration was determined. Conclusions. It is shown that under the action of water deficit there is a decrease in productivity for all studied plants. In this case, genetically modified plants had an advantage in the main elements of the crop structure over the original form under both analyzed growing conditions. It was found that under normal conditions of hydration, the amount of protein in the studied plants did not differ significantly. An increase in its content in control plants under the action of osmotic stress was recorded, which may indicate the synthesis of stress response proteins. Decreased protein content during rehydration may indicate the passage of normal metabolic processes. Keywords: Triticum aestivum L., biotechnological plants, proline dehydrogenase gene, structural analysis of yield, osmostability.
{"title":"Study of transgen functionality in T2 biotechnological plants of winter wheat on the sign of osmoresistance","authors":"A. Komisarenko, S. Mykhalska, V. M. Kurchii","doi":"10.7124/feeo.v28.1381","DOIUrl":"https://doi.org/10.7124/feeo.v28.1381","url":null,"abstract":"Aim. To investigate the functionality of the transgene in the seed generation (T2) of genetically modified winter wheat plants (Triticum aestivum L.) in terms of productivity in conditions of normal and insufficient water supply. Methods. Determination of yield structure and protein content. Results. The indicators of productivity of control and T2 biotechnological plants under normal growing conditions and water deficit are analyzed. The quantitative composition of protein in the conditions before and after osmotic stress and in the period of rehydration was determined. Conclusions. It is shown that under the action of water deficit there is a decrease in productivity for all studied plants. In this case, genetically modified plants had an advantage in the main elements of the crop structure over the original form under both analyzed growing conditions. It was found that under normal conditions of hydration, the amount of protein in the studied plants did not differ significantly. An increase in its content in control plants under the action of osmotic stress was recorded, which may indicate the synthesis of stress response proteins. Decreased protein content during rehydration may indicate the passage of normal metabolic processes. \u0000Keywords: Triticum aestivum L., biotechnological plants, proline dehydrogenase gene, structural analysis of yield, osmostability.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90535233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The investigation of the influence of the constituents of Izatison, DMSO and PEG400, on the oat growth and its grain productivity during 2 generations after the seed treatment became the aim of this study for the elucidation of mechanism of Izatison stimulant influence. Methods. The broad scale of the concentrations of the solvents composition in the proportion, to be as in Izatison, was used. The plants were grown in the field. The growth processes were evaluated by the stem height on the tubing stage. The grain productivity was characterized by the panicle length, the grains number in the panicle and of theirs weight. Results. The dependence of influence of the solvents composition on the stem growth from its concentration was detected in the first generation. In some variants the negative influence was revealed on the grains number in the panicle and on theirs weight. The stimulation of the growth regardless from the solvents concentration was observed in the second generation and the more pronounced negative effect on the grain productivity. Conclusions. The solvents DMSO and PEG400 are able to influence on the oat growth and its productivity, and that effect was manifested also in 2 generation after the seed treatment. Keywords: Izatison, DMSO, PEG400, oat growth and grain productivity.
{"title":"Influence of the constituents of Izatison, DMSO and PEG400, on the growth and the grain productivity of the oat cultivar Nezlamny during two generations after the seed treatment","authors":"V. Katsan, A. Potopalsky, B. O. Zadorozhnii","doi":"10.7124/feeo.v29.1404","DOIUrl":"https://doi.org/10.7124/feeo.v29.1404","url":null,"abstract":"Aim. The investigation of the influence of the constituents of Izatison, DMSO and PEG400, on the oat growth and its grain productivity during 2 generations after the seed treatment became the aim of this study for the elucidation of mechanism of Izatison stimulant influence. Methods. The broad scale of the concentrations of the solvents composition in the proportion, to be as in Izatison, was used. The plants were grown in the field. The growth processes were evaluated by the stem height on the tubing stage. The grain productivity was characterized by the panicle length, the grains number in the panicle and of theirs weight. Results. The dependence of influence of the solvents composition on the stem growth from its concentration was detected in the first generation. In some variants the negative influence was revealed on the grains number in the panicle and on theirs weight. The stimulation of the growth regardless from the solvents concentration was observed in the second generation and the more pronounced negative effect on the grain productivity. Conclusions. The solvents DMSO and PEG400 are able to influence on the oat growth and its productivity, and that effect was manifested also in 2 generation after the seed treatment. \u0000Keywords: Izatison, DMSO, PEG400, oat growth and grain productivity.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87510085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Obtaining and analysis of cytogenetic parameters in meristem cells of Allium cepa L. seedling sprouting from unirradiated seeds, while germinating them with seeds, that were exposed to γ-radiation in different doses. Methods. Cytogenetic analysis included analysis of mitotic activity, anaphase method of chromosome mutations, nucleolar analysis. The F-test was used to compare the control and experimental samples. Results. The formation of the bystander effect is shown in the case of joint germination of irradiated and non-irradiated seeds. The severity of bystander effect increases with increasing irradiation dose. Conclusions. Irradiation of seeds with a dose of gamma radiation of 40 Gy causes the formation of radiation hormesis by the criterion of mitotic activity in the root meristem cells of seedlings, and the irradiation with a dose of 10 Gy does not affect the level of cell proliferation. The bystander effect formation occurs during the joint germination of intact and irradiated seeds as an increase in the level of mitotic activity, and an increase in the number of chromosomal mutations. Keywords: bystander effect, γ-radiation, meristem, mitotic activity, chromosomal abnormalities, micronuclei.
{"title":"The bystander effect formation in the meristem of Allium cepa L. plants with different genotype","authors":"O. German, A. M. Bratchenko, Ye. O. Lytovchenko","doi":"10.7124/feeo.v28.1368","DOIUrl":"https://doi.org/10.7124/feeo.v28.1368","url":null,"abstract":"Aim. Obtaining and analysis of cytogenetic parameters in meristem cells of Allium cepa L. seedling sprouting from unirradiated seeds, while germinating them with seeds, that were exposed to γ-radiation in different doses. Methods. Cytogenetic analysis included analysis of mitotic activity, anaphase method of chromosome mutations, nucleolar analysis. The F-test was used to compare the control and experimental samples. Results. The formation of the bystander effect is shown in the case of joint germination of irradiated and non-irradiated seeds. The severity of bystander effect increases with increasing irradiation dose. Conclusions. Irradiation of seeds with a dose of gamma radiation of 40 Gy causes the formation of radiation hormesis by the criterion of mitotic activity in the root meristem cells of seedlings, and the irradiation with a dose of 10 Gy does not affect the level of cell proliferation. The bystander effect formation occurs during the joint germination of intact and irradiated seeds as an increase in the level of mitotic activity, and an increase in the number of chromosomal mutations. \u0000Keywords: bystander effect, γ-radiation, meristem, mitotic activity, chromosomal abnormalities, micronuclei.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77779547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To determine genealogical and cytogenetic features in girls aged 12-17 years with secondary amenorrhea. Methods. The analysis of pedigrees was conducted in 25 families of girls with secondary amenorrhea (main group) and in 25 families of healthy girls in the laboratory of medical genetics of SI "ICAHC NAMS". Cytogenetic analysis was carried out in the blood lymphocytes of the girls of the main and control groups in vitro. The control group consisted of 25 healthy peer girls with a regular menstrual cycle without somatic pathology. The data obtained were analyzed statistically using the Student's t-test in Excel programs. Results. The hereditary burden on non-inflammatory (hormone-dependent) gynecological diseases was found in 60.0% of families, in 86.6% of cases – along the maternal line, in 6.7% – along the paternal line, in 6.7% – on both lines at the same time; 40.0% of girls had no hereditary burden. The total incidence of gynecological (non-inflammatory) diseases among relatives of three degrees of kinship was 13.6%, which was almost three times higher than the frequency in relatives of healthy girls (5.1%, p < 0.001). Cytogenetic analysis conducted in girls of the main group showed an increase in both the overall level of chromosomal disorders (6.2%), and their individual types (3.2%. 3.0%, 1.56%) compared to the frequency in healthy girls. Conclusions. Family accumulation of gynecological (non-inflammatory) diseases in the pedigree of girls with secondary amenorrhea has been established. Cytogenetic features in the blood lymphocytes of sick girls are revealed compared to healthy peers. Keywords: girls, pedigrees, cytogenetic indices, secondary amenorrhea.
{"title":"Secondary amenorrhea in girls: genealogical and cytogenetic features","authors":"N. Bagatska, V. E. Nefidova, S.V. Novohatska","doi":"10.7124/feeo.v29.1421","DOIUrl":"https://doi.org/10.7124/feeo.v29.1421","url":null,"abstract":"Aim. To determine genealogical and cytogenetic features in girls aged 12-17 years with secondary amenorrhea. Methods. The analysis of pedigrees was conducted in 25 families of girls with secondary amenorrhea (main group) and in 25 families of healthy girls in the laboratory of medical genetics of SI \"ICAHC NAMS\". Cytogenetic analysis was carried out in the blood lymphocytes of the girls of the main and control groups in vitro. The control group consisted of 25 healthy peer girls with a regular menstrual cycle without somatic pathology. The data obtained were analyzed statistically using the Student's t-test in Excel programs. Results. The hereditary burden on non-inflammatory (hormone-dependent) gynecological diseases was found in 60.0% of families, in 86.6% of cases – along the maternal line, in 6.7% – along the paternal line, in 6.7% – on both lines at the same time; 40.0% of girls had no hereditary burden. The total incidence of gynecological (non-inflammatory) diseases among relatives of three degrees of kinship was 13.6%, which was almost three times higher than the frequency in relatives of healthy girls (5.1%, p < 0.001). Cytogenetic analysis conducted in girls of the main group showed an increase in both the overall level of chromosomal disorders (6.2%), and their individual types (3.2%. 3.0%, 1.56%) compared to the frequency in healthy girls. Conclusions. Family accumulation of gynecological (non-inflammatory) diseases in the pedigree of girls with secondary amenorrhea has been established. Cytogenetic features in the blood lymphocytes of sick girls are revealed compared to healthy peers. \u0000Keywords: girls, pedigrees, cytogenetic indices, secondary amenorrhea.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72847938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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