A. Kvasko, A. S. Lazarets, S. Isayenkov, A. Yemets
Aim. The aim of the study was the obtaining of Camelina sativa (L.) Crantz lines with yeast genes of trehalose synthesis TPS1 and TPS2 to increase their resistance to drought. Methods. Seeds of C. sativa genotype FEORZhYaF-1 were used for in vitro culture establishment. For this hypocotyl segments and shoot meristems of 5-days-old camelina seedlings were cultivated on three different nutrient media for regeneration supplemented with various hormone combinations. Vector constructions pGWB2-TPS1 and pGWB2-TPS2 with TPS1 and TPS2 genes have been used for genetic transformation. Results. The highest efficiency of plant regeneration from hypocotyl explants was found on medium supplemented with 1 mg/l BAP and 0.1 mg/l NAA, and from meristem explants – on medium with 1.5 mg/l BAP and 0.5 mg/l NAA. Agrobacterium-mediated transformation was conducted out, and camelina lines were picked up on corresponding medium with selective concentration of hygromycin. Transgenic nature of obtained plants was confirmed by PCR-analysis. Conclusions. The efficiency of in vitro plant regeneration of C. sativa genotype FEORZhYaF-1 has been investigated. Two types of explants and two vector constructions pGWB2-TPS1 and pGWB2-TPS2 with TPS1 and TPS2 yeast trehalose synthesis genes have been used for obtaining of transgenic camelina lines.
{"title":"Agrobacterium-mediated transformation of spring camelina with yeast genes of trehalose biosynthesis","authors":"A. Kvasko, A. S. Lazarets, S. Isayenkov, A. Yemets","doi":"10.7124/feeo.v30.1465","DOIUrl":"https://doi.org/10.7124/feeo.v30.1465","url":null,"abstract":"Aim. The aim of the study was the obtaining of Camelina sativa (L.) Crantz lines with yeast genes of trehalose synthesis TPS1 and TPS2 to increase their resistance to drought. Methods. Seeds of C. sativa genotype FEORZhYaF-1 were used for in vitro culture establishment. For this hypocotyl segments and shoot meristems of 5-days-old camelina seedlings were cultivated on three different nutrient media for regeneration supplemented with various hormone combinations. Vector constructions pGWB2-TPS1 and pGWB2-TPS2 with TPS1 and TPS2 genes have been used for genetic transformation. Results. The highest efficiency of plant regeneration from hypocotyl explants was found on medium supplemented with 1 mg/l BAP and 0.1 mg/l NAA, and from meristem explants – on medium with 1.5 mg/l BAP and 0.5 mg/l NAA. Agrobacterium-mediated transformation was conducted out, and camelina lines were picked up on corresponding medium with selective concentration of hygromycin. Transgenic nature of obtained plants was confirmed by PCR-analysis. Conclusions. The efficiency of in vitro plant regeneration of C. sativa genotype FEORZhYaF-1 has been investigated. Two types of explants and two vector constructions pGWB2-TPS1 and pGWB2-TPS2 with TPS1 and TPS2 yeast trehalose synthesis genes have been used for obtaining of transgenic camelina lines.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"294 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74541072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The aim of this research was to determine the current state of genetic resources of camelina (Camelina sativa) available in Ukraine, and to assess the prospects of its breeding and cultivation. Methods. A comprehensive review of available research was conducted, as well as resources containing data on cultivation scale of camelina and the availability of its genetic resources were assessed. Results. It was found that majority of the existing varieties of spring camelina appear to be close genetically related, while currently there are no winter camelina varieties in Ukraine. It has been established that insignificant scale of camelina cultivation in Ukraine may be associated with low productivity of existing genotypes. Prospects of double-cropping of camelina with other agricultural plants are considered, and also the main approaches for further camelina breeding are drawn. Conclusions. Breeding of camelina winter forms possess a great interest, as they have higher productivity and are better suited for rotations with other crops, common in Ukraine, including oilseeds such as sunflower. It was found that further camelina breeding will require the involvement of new genetic resources to overcome the limited genetic diversity among existing varieties.
{"title":"Current state and perspectives of false flax (Camelina sativa) cultivation in Ukraine","authors":"R. Blume","doi":"10.7124/feeo.v31.1480","DOIUrl":"https://doi.org/10.7124/feeo.v31.1480","url":null,"abstract":"Aim. The aim of this research was to determine the current state of genetic resources of camelina (Camelina sativa) available in Ukraine, and to assess the prospects of its breeding and cultivation. Methods. A comprehensive review of available research was conducted, as well as resources containing data on cultivation scale of camelina and the availability of its genetic resources were assessed. Results. It was found that majority of the existing varieties of spring camelina appear to be close genetically related, while currently there are no winter camelina varieties in Ukraine. It has been established that insignificant scale of camelina cultivation in Ukraine may be associated with low productivity of existing genotypes. Prospects of double-cropping of camelina with other agricultural plants are considered, and also the main approaches for further camelina breeding are drawn. Conclusions. Breeding of camelina winter forms possess a great interest, as they have higher productivity and are better suited for rotations with other crops, common in Ukraine, including oilseeds such as sunflower. It was found that further camelina breeding will require the involvement of new genetic resources to overcome the limited genetic diversity among existing varieties.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89847079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Study the effect of sodium chloride on the physiological and biochemical characteristics of wheat and maize genotypes, to identify varieties tolerant to salinity. To create new salt-tolerant varieties of wheat and maize, a comparative analysis of the physiological and biochemical parameters of the parental and hybrid plants was carried out. Methods. Physiological parameters such as chlorophyll content, relative water content, and PSII activity have been studied in parental forms and hybrids. Results. When studying salt-tolerance of hybrids and parental forms, differences were detected in the relative amounts of chlorophyll (a+b), carotenoids, RWC, as well as the photochemical activity of chloroplasts. The effect of salt on the amount of chlorophyll a, chlorophyll b, and carotenoids, which are the main physiological indicators, is manifested in different ways in both hybrids and parental forms. Conclusions. Based on the physiological indices determined during the grain filling stage, the highest tolerance was manifested by the parental forms, Garabagh, Gobustan, and the hybrids, ♀Garabagh×♂Gobustan, ♀Garabagh×♂Mirbashir, ♀Garabagh×♂Sharg. Under the action of salt in all genotypes of corn found a slight increase in the content of soluble sugars. The highest sugar content was observed in the hybrid Zagatala 68 x Gurur.
{"title":"Evaluation of physiological, biochemical and yield indicators of wheat and maize genotypes exposed to sodium chloride","authors":"M. A. Khanishova, K. R. Tagieva, I. Azizov","doi":"10.7124/feeo.v31.1494","DOIUrl":"https://doi.org/10.7124/feeo.v31.1494","url":null,"abstract":"Aim. Study the effect of sodium chloride on the physiological and biochemical characteristics of wheat and maize genotypes, to identify varieties tolerant to salinity. To create new salt-tolerant varieties of wheat and maize, a comparative analysis of the physiological and biochemical parameters of the parental and hybrid plants was carried out. Methods. Physiological parameters such as chlorophyll content, relative water content, and PSII activity have been studied in parental forms and hybrids. Results. When studying salt-tolerance of hybrids and parental forms, differences were detected in the relative amounts of chlorophyll (a+b), carotenoids, RWC, as well as the photochemical activity of chloroplasts. The effect of salt on the amount of chlorophyll a, chlorophyll b, and carotenoids, which are the main physiological indicators, is manifested in different ways in both hybrids and parental forms. Conclusions. Based on the physiological indices determined during the grain filling stage, the highest tolerance was manifested by the parental forms, Garabagh, Gobustan, and the hybrids, ♀Garabagh×♂Gobustan, ♀Garabagh×♂Mirbashir, ♀Garabagh×♂Sharg. Under the action of salt in all genotypes of corn found a slight increase in the content of soluble sugars. The highest sugar content was observed in the hybrid Zagatala 68 x Gurur.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"138 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86506091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. H. Sakharova, R. Blume, A. Rabokon, Y. Pirko, Y. Blume
Aim. The aim of this research was to compare the efficiency of DNA isolation methods from herbarium specimens of Camelina microcarpa Andrz. Ex DC., further modification of these methods to increase DNA yield, and determine the method that would provide the best yield of isolated DNA. Methods. Modifications of the DNA isolation methods using the DNeasy Plant Mini Kit (QIAgen) and the CTAB method were used. PCR was performed using degenerate primers for method of β-tubulin intron length polymorphism (TBP). Amplicons were fractionated in polyacrylamide gel followed by visualization by silver nitrate staining. Results. DNA was successfully extracted from C. microcarpa herbarium specimens sampled with leaf parts and seeds, using the modified by CTAB method, and four modified methods using DNeasy Plant Mini Kit (QIAgen). Conclusions. The study revealed that the most effective method tested was the DNeasy Plant Mini Kit (QIAgen) No. 2. Prolongation of the cell lysis stage had the best effect on the increase of DNA yield. We found that the success of DNA isolation was influenced not so much by the age of the herbarium specimen as by the methods of drying and storing the plants in the collection.
的目标。本研究的目的是比较不同方法对小茶树标本DNA分离的效率。前女友。，进一步修改这些方法以提高DNA产量，并确定可以提供最佳分离DNA产量的方法。方法。采用DNeasy Plant Mini Kit (QIAgen)和CTAB法对DNA分离方法进行了改进。采用退化引物进行β-微管蛋白内含子长度多态性(TBP)方法的PCR检测。扩增子在聚丙烯酰胺凝胶中分离，然后用硝酸银染色显示。结果。采用CTAB法和DNeasy Plant Mini Kit (QIAgen)的4种修饰方法，成功提取了小叶柏(C. microcarpa)标本叶部和种子的DNA。结论。研究表明，最有效的方法是DNeasy Plant Mini Kit (QIAgen) No. 2。延长细胞裂解期对DNA产率的提高效果最好。我们发现，DNA分离的成功与否与其说是受植物标本室标本年龄的影响，不如说是受植物标本干燥和储存方法的影响。
{"title":"Comparison of methods of DNA extraction from herbarium specimens of little-pod false flax (Camelina microcarpa Andrz. Ex Dc.)","authors":"V. H. Sakharova, R. Blume, A. Rabokon, Y. Pirko, Y. Blume","doi":"10.7124/feeo.v30.1457","DOIUrl":"https://doi.org/10.7124/feeo.v30.1457","url":null,"abstract":"Aim. The aim of this research was to compare the efficiency of DNA isolation methods from herbarium specimens of Camelina microcarpa Andrz. Ex DC., further modification of these methods to increase DNA yield, and determine the method that would provide the best yield of isolated DNA. Methods. Modifications of the DNA isolation methods using the DNeasy Plant Mini Kit (QIAgen) and the CTAB method were used. PCR was performed using degenerate primers for method of β-tubulin intron length polymorphism (TBP). Amplicons were fractionated in polyacrylamide gel followed by visualization by silver nitrate staining. Results. DNA was successfully extracted from C. microcarpa herbarium specimens sampled with leaf parts and seeds, using the modified by CTAB method, and four modified methods using DNeasy Plant Mini Kit (QIAgen). Conclusions. The study revealed that the most effective method tested was the DNeasy Plant Mini Kit (QIAgen) No. 2. Prolongation of the cell lysis stage had the best effect on the increase of DNA yield. We found that the success of DNA isolation was influenced not so much by the age of the herbarium specimen as by the methods of drying and storing the plants in the collection.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75362659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Investigation of allele frequencies by locus of reserve proteins in groups of wheat varieties created during different selection periods at the leading breeding center of the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine. Methods. The material for the study were 57 varieties of wheat of the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine. Separation of gliadins was performed according to the ISTA method in the Poperel modification. High molecular weight glutenin subunits were analyzed by electrophoresis in the presence of sodium dodecyl sulfate (SDS) by the Lemmley method. Results. The allelic composition of loci of spare proteins Glu-A1, Glu-B1, Glu-D1, Gli-1A, Gli-B1, Gli-1D was determined. At the gliad-encoding loci Gli-A1, Gli-B1 6 alleles were detected, at the locus Gli-D1 - 5 alleles. By gluten-encoding loci, the most polymorphic was the Glu-B1 locus, which is represented by 5 alleles. Loci Glu-A1 and Glu-D1 are represented by 3 and 2 allelic variants. Conclusions. The frequency of the Glu-B1al allele, increased from 0% to 18%, this allele is one of the strongest in terms of positive impact on the quality of flour among the identified alleles of loci of high molecular weight glutenins of wheat. It was found that almost 50% of varieties created in the last decade in IFRG NAS of Ukraine have wheat-rye translocations 1AL.1RS, 1BL.1RS.
{"title":"Diversity of alleles of locuses of spare proteins of wheat varieties IFRG NAS of Ukraine","authors":"N. V. Sandetska, O. M. Radchenko","doi":"10.7124/feeo.v30.1456","DOIUrl":"https://doi.org/10.7124/feeo.v30.1456","url":null,"abstract":"Aim. Investigation of allele frequencies by locus of reserve proteins in groups of wheat varieties created during different selection periods at the leading breeding center of the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine. Methods. The material for the study were 57 varieties of wheat of the Institute of Plant Physiology and Genetics of the National Academy of Sciences of Ukraine. Separation of gliadins was performed according to the ISTA method in the Poperel modification. High molecular weight glutenin subunits were analyzed by electrophoresis in the presence of sodium dodecyl sulfate (SDS) by the Lemmley method. Results. The allelic composition of loci of spare proteins Glu-A1, Glu-B1, Glu-D1, Gli-1A, Gli-B1, Gli-1D was determined. At the gliad-encoding loci Gli-A1, Gli-B1 6 alleles were detected, at the locus Gli-D1 - 5 alleles. By gluten-encoding loci, the most polymorphic was the Glu-B1 locus, which is represented by 5 alleles. Loci Glu-A1 and Glu-D1 are represented by 3 and 2 allelic variants. Conclusions. The frequency of the Glu-B1al allele, increased from 0% to 18%, this allele is one of the strongest in terms of positive impact on the quality of flour among the identified alleles of loci of high molecular weight glutenins of wheat. It was found that almost 50% of varieties created in the last decade in IFRG NAS of Ukraine have wheat-rye translocations 1AL.1RS, 1BL.1RS.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79510709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hordynskyi, A. Postovoitova, A. Rabokon, Y. Pirko, Y. Blume
To develop marker systems based on the study of intron length polymorphism of various genes (ILP) in Ae. tauschii, to test the possibility of their use for genetic differentiation of Ae. tauschii and Ae. biuncialis. Methods. The NCBI database was used to obtain EST sequences, online tools CD-HIT, BLAST and Primer3Plus (for the development of primers). The polymerase chain reaction (PCR) method with the developed primers was done. Amplified fragments were separated by non-denaturing polyacrylamide gel electrophoresis and stained with silver. Results. Molecular markers Aet_ILP1, Aet_ILP6 were developed and tested for different genotypes of Ae. tauschii and Ae. biuncialis. No intraspecific differentiation was observed in all studied samples, but amplicons of introns of different species differed significantly. It was also found that the studied species had one copy of the HO222074.1 gene and 1 to 3 copies of the CX244643.1 gene. Conclusions. The obtained results indicate a low level of intraspecific variability of the developed ILP markers and the possibility of their use for interspecific differentiation of Ae. tauschii and Ae. biuncialis.
{"title":"Development of ILP-markers for Aegilops tauschii and their application in molecular genetic analysis","authors":"S. Hordynskyi, A. Postovoitova, A. Rabokon, Y. Pirko, Y. Blume","doi":"10.7124/feeo.v30.1455","DOIUrl":"https://doi.org/10.7124/feeo.v30.1455","url":null,"abstract":"To develop marker systems based on the study of intron length polymorphism of various genes (ILP) in Ae. tauschii, to test the possibility of their use for genetic differentiation of Ae. tauschii and Ae. biuncialis. Methods. The NCBI database was used to obtain EST sequences, online tools CD-HIT, BLAST and Primer3Plus (for the development of primers). The polymerase chain reaction (PCR) method with the developed primers was done. Amplified fragments were separated by non-denaturing polyacrylamide gel electrophoresis and stained with silver. Results. Molecular markers Aet_ILP1, Aet_ILP6 were developed and tested for different genotypes of Ae. tauschii and Ae. biuncialis. No intraspecific differentiation was observed in all studied samples, but amplicons of introns of different species differed significantly. It was also found that the studied species had one copy of the HO222074.1 gene and 1 to 3 copies of the CX244643.1 gene. Conclusions. The obtained results indicate a low level of intraspecific variability of the developed ILP markers and the possibility of their use for interspecific differentiation of Ae. tauschii and Ae. biuncialis.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"78 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91478945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The investigation was aimed to compare the efficiency of mannitol as a component of spike cold pretreatment solution and of medium for spring barley haploid production via anther culture in vitro. Methods. Field-grown plants of two genotypes with a contrast androgenic capacity were used as donors. Cut tillers were pretreated in water at 4⁰C for 5 days, while aseptically isolated spikes were kept in 0.3 M mannitol for 10 days at the same temperature. Isolated anthers were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose (9.0%); the media with and without 0.1M mannitol differed in solidifying agents. Results. In highly-responsive line DH00-126, there was an almost two-fold increase in mmgreen plant regeneration frequency (from 48 to 58 plants per 100 anthers) in all experimental variants in comparison to the control. In recalcitrant variety Mebere, the combination of tiller pretreatment in water with anther cultivation on inductive starch-gelling media supplemented with 0.1 M mannitol resulted in the highest green plant yield. Conclusions. Both methodical approaches were proved to be applicable to increase the efficiency of haploid production in spring barley anther culture in vitro.
{"title":"Use of mannitol for cold pretreatment of isolated spikes and as a component of nutrient medium for spring barley anther culture in vitro","authors":"O. Bilynska","doi":"10.7124/feeo.v30.1459","DOIUrl":"https://doi.org/10.7124/feeo.v30.1459","url":null,"abstract":"Aim. The investigation was aimed to compare the efficiency of mannitol as a component of spike cold pretreatment solution and of medium for spring barley haploid production via anther culture in vitro. Methods. Field-grown plants of two genotypes with a contrast androgenic capacity were used as donors. Cut tillers were pretreated in water at 4⁰C for 5 days, while aseptically isolated spikes were kept in 0.3 M mannitol for 10 days at the same temperature. Isolated anthers were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose (9.0%); the media with and without 0.1M mannitol differed in solidifying agents. Results. In highly-responsive line DH00-126, there was an almost two-fold increase in mmgreen plant regeneration frequency (from 48 to 58 plants per 100 anthers) in all experimental variants in comparison to the control. In recalcitrant variety Mebere, the combination of tiller pretreatment in water with anther cultivation on inductive starch-gelling media supplemented with 0.1 M mannitol resulted in the highest green plant yield. Conclusions. Both methodical approaches were proved to be applicable to increase the efficiency of haploid production in spring barley anther culture in vitro.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87793799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Kozub, I. Sozinov, H. Bidnyk, N. Demianova, O. Sozinova, A. Karelov, S. Spivak, Y. Blume
Aim. The study was aimed at identification and selection of winter common wheat genotypes with null-alleles at the gliadin loci Gli-B1 and Gli-D1. Methods. The search for spontaneous mutants with the absence of synthesis of gliadin blocks was made in winter common wheat hybrid material and cultivars. To identify mutations, APAG electrophoresis and SDS-electrophoresis of seed storage proteins were performed. Results. The frequency of spontaneous mutations resulting in the null-allele at the loci Gli-B1 and Gli-D1 in F2 was 0,1 % and 0,05 %, respectively, in the cross Odesska chervonokolosa × B-16. Via sowing the material with those mutations and marker selection, F6 lines were developed: the line OB-Bnull with the null-allele at the Gli-B1 locus and the line OB-Dnull with the null-allele at the Gli-D1 locus. Another line with the null-allele at Gli-B1 was produced from the cross B3 × D4 by marker selection, as well as a biotype with the null-allele at the Gli-D1 locus was selected from the cultivar Slaven. Conclusions. The developed lines with null-alleles at the gliadin locus Gli-B1 (without omega-5 gliadins) and Gli-D1 (without omega-1,2 gliadins) are initial material for hypoallergenic wheat breeding.
{"title":"Common wheat genotypes with null-alleles at gliadin loci","authors":"N. Kozub, I. Sozinov, H. Bidnyk, N. Demianova, O. Sozinova, A. Karelov, S. Spivak, Y. Blume","doi":"10.7124/feeo.v31.1485","DOIUrl":"https://doi.org/10.7124/feeo.v31.1485","url":null,"abstract":"Aim. The study was aimed at identification and selection of winter common wheat genotypes with null-alleles at the gliadin loci Gli-B1 and Gli-D1. Methods. The search for spontaneous mutants with the absence of synthesis of gliadin blocks was made in winter common wheat hybrid material and cultivars. To identify mutations, APAG electrophoresis and SDS-electrophoresis of seed storage proteins were performed. Results. The frequency of spontaneous mutations resulting in the null-allele at the loci Gli-B1 and Gli-D1 in F2 was 0,1 % and 0,05 %, respectively, in the cross Odesska chervonokolosa × B-16. Via sowing the material with those mutations and marker selection, F6 lines were developed: the line OB-Bnull with the null-allele at the Gli-B1 locus and the line OB-Dnull with the null-allele at the Gli-D1 locus. Another line with the null-allele at Gli-B1 was produced from the cross B3 × D4 by marker selection, as well as a biotype with the null-allele at the Gli-D1 locus was selected from the cultivar Slaven. Conclusions. The developed lines with null-alleles at the gliadin locus Gli-B1 (without omega-5 gliadins) and Gli-D1 (without omega-1,2 gliadins) are initial material for hypoallergenic wheat breeding.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87942944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Tarasiuk, O. Stasik, G. Priadkina, D. Konovalov
Aim. Evaluation of the role of different internodes and leaf sheaths of winter wheat stem in storage and remobilization of reserve water-soluble carbohydrates (WSC) under natural water deficit in order to develop effective criteria for selection of high-yielding genotypes. Methods. The specific content and total amount of WSC at the onset and the end of grain filling and the quantity of WSC remobilized in different parts of the stem were determined. Results. It was found that the main part of reserve WSC in plants of modern winter wheat varieties was accumulated and mobilized in the 2nd, 3rd and 4-5th (counting from above) internodes, the contribution of peduncle and leaf sheaths was much smaller. The highest WSC depositing capacity of all stem segments were found in the high-yielding variety Kyivska 17 and the lowest one in the less productive variety Poradnytsia. Conclusions. The values of accumulation and remobilization of WSC in the 2nd and 3rd from the top internodes can characterize the storage capacity of the stem and be used to assess the relationships with grain productivity in the breeding winter wheat in arid conditions.
{"title":"The role of different stem segments in the deposition of water-soluble carbohydrates in winter wheat varieties under drought conditions","authors":"M. Tarasiuk, O. Stasik, G. Priadkina, D. Konovalov","doi":"10.7124/feeo.v31.1499","DOIUrl":"https://doi.org/10.7124/feeo.v31.1499","url":null,"abstract":"Aim. Evaluation of the role of different internodes and leaf sheaths of winter wheat stem in storage and remobilization of reserve water-soluble carbohydrates (WSC) under natural water deficit in order to develop effective criteria for selection of high-yielding genotypes. Methods. The specific content and total amount of WSC at the onset and the end of grain filling and the quantity of WSC remobilized in different parts of the stem were determined. Results. It was found that the main part of reserve WSC in plants of modern winter wheat varieties was accumulated and mobilized in the 2nd, 3rd and 4-5th (counting from above) internodes, the contribution of peduncle and leaf sheaths was much smaller. The highest WSC depositing capacity of all stem segments were found in the high-yielding variety Kyivska 17 and the lowest one in the less productive variety Poradnytsia. Conclusions. The values of accumulation and remobilization of WSC in the 2nd and 3rd from the top internodes can characterize the storage capacity of the stem and be used to assess the relationships with grain productivity in the breeding winter wheat in arid conditions.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74592284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. Investigate the effect of Agrobacterium-mediated transformation in planta on seed tying and the frequency of transformation in winter wheat (Triticum aestivum L.). To analyze changes in the level of free L-proline (Pro) in transformed and control seedlings under normal / stress conditions and productivity indicators of biotechnological plants (T1) under normal growing conditions. Methods. Agrobacterium-mediated transformation in planta; PCR analysis, DNA electrophoresis; determination of seed tying frequency and transformation, Pro content, yield structure indicators. Results. Obtained transgenic wheat plants. The level of Pro in the tested variants under normal / stress conditions and indicators of T1 productivity of plants and their initial form under optimal water supply were studied. Conclusions. The susceptibility of the studied wheat genotypes to agrobacterial infection is shown. The frequency of seed tying after genetic transformation was 12.7 % and 5.4 % for plants of UK 106/19 and UK 171/19h, respectively. Transgenic seedlings had elevated levels of Pro. Complete incorporation of the vector construct was identified in 14 and 11 variants of genotypes UK 161/19 and UK 171/19h, respectively. Control and T1 biotechnological plants under normal growing conditions had similar yields.
{"title":"Agrobacterium-mediated transformation – method of genetic modification of Triticum aestivum L. plants","authors":"A. Komisarenko, S. Mykhalska, V. M. Kurchii","doi":"10.7124/feeo.v30.1466","DOIUrl":"https://doi.org/10.7124/feeo.v30.1466","url":null,"abstract":"Aim. Investigate the effect of Agrobacterium-mediated transformation in planta on seed tying and the frequency of transformation in winter wheat (Triticum aestivum L.). To analyze changes in the level of free L-proline (Pro) in transformed and control seedlings under normal / stress conditions and productivity indicators of biotechnological plants (T1) under normal growing conditions. Methods. Agrobacterium-mediated transformation in planta; PCR analysis, DNA electrophoresis; determination of seed tying frequency and transformation, Pro content, yield structure indicators. Results. Obtained transgenic wheat plants. The level of Pro in the tested variants under normal / stress conditions and indicators of T1 productivity of plants and their initial form under optimal water supply were studied. Conclusions. The susceptibility of the studied wheat genotypes to agrobacterial infection is shown. The frequency of seed tying after genetic transformation was 12.7 % and 5.4 % for plants of UK 106/19 and UK 171/19h, respectively. Transgenic seedlings had elevated levels of Pro. Complete incorporation of the vector construct was identified in 14 and 11 variants of genotypes UK 161/19 and UK 171/19h, respectively. Control and T1 biotechnological plants under normal growing conditions had similar yields.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84464001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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