S. Hordynskyi, A. Postovoitova, A. Rabokon, Y. Pirko, Y. Blume
To develop marker systems based on the study of intron length polymorphism of various genes (ILP) in Ae. tauschii, to test the possibility of their use for genetic differentiation of Ae. tauschii and Ae. biuncialis. Methods. The NCBI database was used to obtain EST sequences, online tools CD-HIT, BLAST and Primer3Plus (for the development of primers). The polymerase chain reaction (PCR) method with the developed primers was done. Amplified fragments were separated by non-denaturing polyacrylamide gel electrophoresis and stained with silver. Results. Molecular markers Aet_ILP1, Aet_ILP6 were developed and tested for different genotypes of Ae. tauschii and Ae. biuncialis. No intraspecific differentiation was observed in all studied samples, but amplicons of introns of different species differed significantly. It was also found that the studied species had one copy of the HO222074.1 gene and 1 to 3 copies of the CX244643.1 gene. Conclusions. The obtained results indicate a low level of intraspecific variability of the developed ILP markers and the possibility of their use for interspecific differentiation of Ae. tauschii and Ae. biuncialis.
{"title":"Development of ILP-markers for Aegilops tauschii and their application in molecular genetic analysis","authors":"S. Hordynskyi, A. Postovoitova, A. Rabokon, Y. Pirko, Y. Blume","doi":"10.7124/feeo.v30.1455","DOIUrl":"https://doi.org/10.7124/feeo.v30.1455","url":null,"abstract":"To develop marker systems based on the study of intron length polymorphism of various genes (ILP) in Ae. tauschii, to test the possibility of their use for genetic differentiation of Ae. tauschii and Ae. biuncialis. Methods. The NCBI database was used to obtain EST sequences, online tools CD-HIT, BLAST and Primer3Plus (for the development of primers). The polymerase chain reaction (PCR) method with the developed primers was done. Amplified fragments were separated by non-denaturing polyacrylamide gel electrophoresis and stained with silver. Results. Molecular markers Aet_ILP1, Aet_ILP6 were developed and tested for different genotypes of Ae. tauschii and Ae. biuncialis. No intraspecific differentiation was observed in all studied samples, but amplicons of introns of different species differed significantly. It was also found that the studied species had one copy of the HO222074.1 gene and 1 to 3 copies of the CX244643.1 gene. Conclusions. The obtained results indicate a low level of intraspecific variability of the developed ILP markers and the possibility of their use for interspecific differentiation of Ae. tauschii and Ae. biuncialis.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"78 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91478945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu. O. Bilonozhko, T. Krupodorova, T. V. Topchii, A. Rabokon, A. Postovoitova, L. Kalafat, S. M. Pryvalikhin, Y. Pirko
Aim. The aim of the study was to investigate the natural antagonists of white mistletoe (Viscum album L.), which may affect the number and speed of spread of this species. Methods. The plant material of V. album, collected in urban plantations of Kyiv, was used in the study. The research was conducted mainly in park zones and urban plantations of Podilskyi district. Samples of affected white mistletoe were collected by the route method during 2019-2021. Plant material was also inspected during the sanitary cutting of utilities. Results. Different species of vertebrates and invertebrates, fungi, lichens and bacteria that form close ecological links with V. album were analyzed. It has been established that several species of fungi can parasitize on V. album, showing a fairly high level of specialization. Hyperparasitic disease has also been reported. Conclusions. A list of species associated with white mistletoe was identified and the possibility of their use in the development of biological methods to control the semi-parasite was assessed.
的目标。本研究的目的是研究白槲寄生(Viscum album L.)的天然拮抗剂对其传播数量和速度的影响。方法。本研究采用采自基辅市区人工林的V. album植物材料。研究主要在Podilskyi地区的公园带和城市人工林进行。2019-2021年采用路径法采集白槲寄生样本。在对公用设施进行卫生切割时也检查了植物材料。结果。分析了不同种类的脊椎动物和无脊椎动物、真菌、地衣和细菌与紫霉形成密切的生态联系。已有研究证实，几种真菌可以寄生于紫霉上，表现出相当高的专门化水平。高寄生虫病也有报道。结论。鉴定了一份与白槲寄生有关的物种清单，并评估了利用这些物种开发控制这种半寄生虫的生物方法的可能性。
{"title":"Phytophages and mycobiots of white mistletoe","authors":"Yu. O. Bilonozhko, T. Krupodorova, T. V. Topchii, A. Rabokon, A. Postovoitova, L. Kalafat, S. M. Pryvalikhin, Y. Pirko","doi":"10.7124/feeo.v31.1478","DOIUrl":"https://doi.org/10.7124/feeo.v31.1478","url":null,"abstract":"Aim. The aim of the study was to investigate the natural antagonists of white mistletoe (Viscum album L.), which may affect the number and speed of spread of this species. Methods. The plant material of V. album, collected in urban plantations of Kyiv, was used in the study. The research was conducted mainly in park zones and urban plantations of Podilskyi district. Samples of affected white mistletoe were collected by the route method during 2019-2021. Plant material was also inspected during the sanitary cutting of utilities. Results. Different species of vertebrates and invertebrates, fungi, lichens and bacteria that form close ecological links with V. album were analyzed. It has been established that several species of fungi can parasitize on V. album, showing a fairly high level of specialization. Hyperparasitic disease has also been reported. Conclusions. A list of species associated with white mistletoe was identified and the possibility of their use in the development of biological methods to control the semi-parasite was assessed.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82239647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. M. Radchenko, H. Andriiash, N. Y. Beiko, O. Tigunova, S. Shulga
Aim. The increasing of riboflavin accumulation by chemical mutagenesis of Bacillus subtilis IMB B-7797 strain producer was the aim of this work. Methods. We used the method of treating the strain producer with a chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine to achieve this goal and followed by culturing the resulting clone and determining the accumulation of riboflavin. Results. B. subtilis IFBG NTG2 mutant strain producer which differed in morphological characteristics (color, size, colony shape) and riboflavin accumulation from the original culture as a result of chemical mutagen action on B. subtilis IMB B-7797 was obtained. Conclusions. B. subtilis IFBG NTG2 mutant strain producer as a result of action on B. subtilis IMB B-7797 by chemical mutagen and which differed in morphological features (color, size, colony shape) and riboflavin accumulation from the original culture. was obtained, B. subtilis IFBG NTG2 strain producer, which produced riboflavin amount of 14.8 g/dm3, which is 9% more than produced by the original B. subtilis IMB B-7797 strain producer was obtained by chemical mutagenesis. B. subtilis IFBG NTG2 strain produser is further proposed for use in industrial technology of riboflavin and creation of a recombinant strain produser for synthesis of riboflavin.
{"title":"Bacillus subtilis strain producer preparation with increased accumulation of riboflavin","authors":"M. M. Radchenko, H. Andriiash, N. Y. Beiko, O. Tigunova, S. Shulga","doi":"10.7124/feeo.v31.1488","DOIUrl":"https://doi.org/10.7124/feeo.v31.1488","url":null,"abstract":"Aim. The increasing of riboflavin accumulation by chemical mutagenesis of Bacillus subtilis IMB B-7797 strain producer was the aim of this work. Methods. We used the method of treating the strain producer with a chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine to achieve this goal and followed by culturing the resulting clone and determining the accumulation of riboflavin. Results. B. subtilis IFBG NTG2 mutant strain producer which differed in morphological characteristics (color, size, colony shape) and riboflavin accumulation from the original culture as a result of chemical mutagen action on B. subtilis IMB B-7797 was obtained. Conclusions. B. subtilis IFBG NTG2 mutant strain producer as a result of action on B. subtilis IMB B-7797 by chemical mutagen and which differed in morphological features (color, size, colony shape) and riboflavin accumulation from the original culture. was obtained, B. subtilis IFBG NTG2 strain producer, which produced riboflavin amount of 14.8 g/dm3, which is 9% more than produced by the original B. subtilis IMB B-7797 strain producer was obtained by chemical mutagenesis. B. subtilis IFBG NTG2 strain produser is further proposed for use in industrial technology of riboflavin and creation of a recombinant strain produser for synthesis of riboflavin.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82357435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Vozhegova, Yu.O. Lavrinenko, T. Marchenko, P. Zabara, V. Bazaliy
Aim. Investigate the influence of treatment of maize plants with restrictive biological products on the formation of productivity of lines - parent components to optimize the elements of cultivation technology. Methods. 3-factor field experiment; measuring and weighing - to determine the structure of the crop; methods of mathematical statistics (analysis of variance, rectilinear and curvilinear correlation and regression). Results. Studies have shown that for the maximum manifestation of the sign "mass of 1000 grains" the optimal density is 70,000 plants ha-1. The increase in yield is positively influenced by the increase in the weight of 1000 seeds, which is due to both the genotype of the lines and the use of biologically active drugs Bio-gel, Helafit-combi. With the use of the drug Bio-gel laboratory germination increased by an average of 1.5 %, with the use of the drug Helafit-combi seed germination increased by 2.4 %. Conclusions. The maximum yield of the parent component DK 247 was observed at a density of 80 thousand plants/ha and treatment with the drug Helafit-combi - 4.89 t/ha. Mid-late lines - parent components DK 411 and DK 445 showed the highest yields at densities of 70,000 plants ha-1 and treatment with Helafit®-combi - 4.65 and 6.30 t/ha, respectively.
{"title":"Productivity of parental inbred lines – components of maize hybrids depending on plant density and treatment with biopreparations under conditions of drip irrigation","authors":"R. Vozhegova, Yu.O. Lavrinenko, T. Marchenko, P. Zabara, V. Bazaliy","doi":"10.7124/feeo.v31.1482","DOIUrl":"https://doi.org/10.7124/feeo.v31.1482","url":null,"abstract":"Aim. Investigate the influence of treatment of maize plants with restrictive biological products on the formation of productivity of lines - parent components to optimize the elements of cultivation technology. Methods. 3-factor field experiment; measuring and weighing - to determine the structure of the crop; methods of mathematical statistics (analysis of variance, rectilinear and curvilinear correlation and regression). Results. Studies have shown that for the maximum manifestation of the sign \"mass of 1000 grains\" the optimal density is 70,000 plants ha-1. The increase in yield is positively influenced by the increase in the weight of 1000 seeds, which is due to both the genotype of the lines and the use of biologically active drugs Bio-gel, Helafit-combi. With the use of the drug Bio-gel laboratory germination increased by an average of 1.5 %, with the use of the drug Helafit-combi seed germination increased by 2.4 %. Conclusions. The maximum yield of the parent component DK 247 was observed at a density of 80 thousand plants/ha and treatment with the drug Helafit-combi - 4.89 t/ha. Mid-late lines - parent components DK 411 and DK 445 showed the highest yields at densities of 70,000 plants ha-1 and treatment with Helafit®-combi - 4.65 and 6.30 t/ha, respectively.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73470093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The aim of research was to investigate influence of water deficit in soil on morphology and productivity of bread winter wheat plants (Triticum aestivum L.). Methods. Wheat plants cultivars Podolyanka and Natalka were grown under optimal conditions until the earing-flowering phase, after that the experimental plants were transferred to drought regime for 8 days. The optimal supply of water was restored to the end of vegetation. The leaf surface area, the mass of internodes and ear were measured during the experiment. Ripened plants were analyzed by the yield structure. Results. It is established that the effect of water deficit in the critical earing-flowering phase of ontogenesis caused decreasing in the leaf surface area, mass of internodes and ear, weight of 1000 grains in both Podolyanka and Natalka cultivars. However, number of grains decreased only in cultivar Podolyanka under drought. Conclusions. Water deficit in the soil in the critical earing-flowering phase led to inhibition of growth processes and productivity reduction in bread winter wheat.
{"title":"Winter wheat productivity formation under water deficit in soil","authors":"O. Zhuk, O. Stasik","doi":"10.7124/feeo.v31.1483","DOIUrl":"https://doi.org/10.7124/feeo.v31.1483","url":null,"abstract":"Aim. The aim of research was to investigate influence of water deficit in soil on morphology and productivity of bread winter wheat plants (Triticum aestivum L.). Methods. Wheat plants cultivars Podolyanka and Natalka were grown under optimal conditions until the earing-flowering phase, after that the experimental plants were transferred to drought regime for 8 days. The optimal supply of water was restored to the end of vegetation. The leaf surface area, the mass of internodes and ear were measured during the experiment. Ripened plants were analyzed by the yield structure. Results. It is established that the effect of water deficit in the critical earing-flowering phase of ontogenesis caused decreasing in the leaf surface area, mass of internodes and ear, weight of 1000 grains in both Podolyanka and Natalka cultivars. However, number of grains decreased only in cultivar Podolyanka under drought. Conclusions. Water deficit in the soil in the critical earing-flowering phase led to inhibition of growth processes and productivity reduction in bread winter wheat.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81362533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The genus Aconitum is represented in the Carpathian region by a significant number of endemic species. Some of these species, in particular A. pseudanthora and A. jacquinii, are included in the Red List of Ukraine. However, their taxonomic status and consequently the importance of conserving their natural resources remain uncertain. DNA barcoding techniques, e.g., the application of molecular markers based on variable regions of the chloroplast genome can help clarify these questions. Methods. DNA isolation, PCR amplification, sequencing of PCR products, phylogenetic analysis. Results. The psbA-trnH intergenic spacer region of A. pseudanthora and A. jacquinii specimens from Western Ukraine was amplified and sequenced. It is shown that the spacer sequences for these two species are identical, but differ in short indels and nucleotide substitutions from the psbA-trnH sequences of A. anthora s.l. specimens from Western Europe and China. Phylogenetic analyzes showed the phylogenetic affinity of the members of the A. anthora complex to the subgenus Lycoctonum. Conclusions. The use of the psbA-trnH intergenic spacer region for DNA barcoding makes it possible to unambiguously distinguish samples of A. jacquinii and A. pseudanthora from western Ukraine from samples of A. anthora s.l. of other geographical origins. The results obtained confirm the reticular nature of the evolution in the genus Aconitum.
{"title":"Utility of the trnH–psbA region for DNA barcoding of Aconitum anthora L. and related taxa","authors":"Y. Tynkevich, D. V. Biliay, R. Volkov","doi":"10.7124/feeo.v31.1500","DOIUrl":"https://doi.org/10.7124/feeo.v31.1500","url":null,"abstract":"Aim. The genus Aconitum is represented in the Carpathian region by a significant number of endemic species. Some of these species, in particular A. pseudanthora and A. jacquinii, are included in the Red List of Ukraine. However, their taxonomic status and consequently the importance of conserving their natural resources remain uncertain. DNA barcoding techniques, e.g., the application of molecular markers based on variable regions of the chloroplast genome can help clarify these questions. Methods. DNA isolation, PCR amplification, sequencing of PCR products, phylogenetic analysis. Results. The psbA-trnH intergenic spacer region of A. pseudanthora and A. jacquinii specimens from Western Ukraine was amplified and sequenced. It is shown that the spacer sequences for these two species are identical, but differ in short indels and nucleotide substitutions from the psbA-trnH sequences of A. anthora s.l. specimens from Western Europe and China. Phylogenetic analyzes showed the phylogenetic affinity of the members of the A. anthora complex to the subgenus Lycoctonum. Conclusions. The use of the psbA-trnH intergenic spacer region for DNA barcoding makes it possible to unambiguously distinguish samples of A. jacquinii and A. pseudanthora from western Ukraine from samples of A. anthora s.l. of other geographical origins. The results obtained confirm the reticular nature of the evolution in the genus Aconitum.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"2012 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86363772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. To establish the dependence of free proline concentration in plant tissues of in vitro and ex vitro alpine species of Gentiana lutea L., Gentiana punctata L., Gentiana acaulis L. on light conditions of their cultivation and the source of carbon in the nutrient medium; to analyze expediency of free proline amino acid use as a biological marker of physiological adaptation of biotechnological plants of these species to water deficit of in vitro and ex vitro conditions. Methods. Methods of in vitro and ex vitro cultivation of plants, the method of free proline detection with the use of ninhydrin. Results. It is shown that under in vitro conditions of free proline content in plants is dependent on light regime of their cultivation and the source of carbon in the composition of nutrient medium. The increased intensity of luminous flux within the range of photosynthetically active radiation (PAR) from 85 W/m2 to 100 W/m2 and 1.92 times raised share of red range waves in the light spectral composition in variant 2.1 (intensity of the luminous flux in the PAR range is 100 W/m2, the waves correlation of blue (Eb): green (Eg): red (Er) ranges = 25 % : 27 % : 48 %) causes 11.5–37.1 % increased amount of free proline in plants in vitro cultivated on nutrient media supplemented with sucrose. Substitution of sucrose in the medium for mannitol is accompanied by 1.64–1.84 times increased concentration of free proline in plants of the investigated species under the light conditions of 1.1 variant (85 W/m2, spectral composition Eb : Eg : Er = 25 % : 27 % : 48 %= 33 % : 42 % : 25 %) and 1.3–2.57 times increased under light cultivation regime of 2.1 variant. The analysis of water balance of plants in vitro cultivated on mannitol under light conditions of 2.1 variant and the plants in conditions of natural growth doesn’t show any considerable distinctions in values of transpiration intensity, water deficit, general water content. The process of adaptation of plants in vitro to conditions ex vitro is accompanied by a change of proline content in leaves with dependence on water deficit in the substrate and light conditions of growing. Conclusions. The obtained results point to the expediency of using free proline content as a biochemical marker for assessing adaptive potential of plants in conditions in vitro and ex vitro.
{"title":"The influence of in vitro and ex vitro conditions cultivation on free proline contents in plants of some Gentiana L. species","authors":"L. Hrytsak, N. Nuzhyna, N. Drobyk","doi":"10.7124/feeo.v30.1461","DOIUrl":"https://doi.org/10.7124/feeo.v30.1461","url":null,"abstract":"Aim. To establish the dependence of free proline concentration in plant tissues of in vitro and ex vitro alpine species of Gentiana lutea L., Gentiana punctata L., Gentiana acaulis L. on light conditions of their cultivation and the source of carbon in the nutrient medium; to analyze expediency of free proline amino acid use as a biological marker of physiological adaptation of biotechnological plants of these species to water deficit of in vitro and ex vitro conditions. Methods. Methods of in vitro and ex vitro cultivation of plants, the method of free proline detection with the use of ninhydrin. Results. It is shown that under in vitro conditions of free proline content in plants is dependent on light regime of their cultivation and the source of carbon in the composition of nutrient medium. The increased intensity of luminous flux within the range of photosynthetically active radiation (PAR) from 85 W/m2 to 100 W/m2 and 1.92 times raised share of red range waves in the light spectral composition in variant 2.1 (intensity of the luminous flux in the PAR range is 100 W/m2, the waves correlation of blue (Eb): green (Eg): red (Er) ranges = 25 % : 27 % : 48 %) causes 11.5–37.1 % increased amount of free proline in plants in vitro cultivated on nutrient media supplemented with sucrose. Substitution of sucrose in the medium for mannitol is accompanied by 1.64–1.84 times increased concentration of free proline in plants of the investigated species under the light conditions of 1.1 variant (85 W/m2, spectral composition Eb : Eg : Er = 25 % : 27 % : 48 %= 33 % : 42 % : 25 %) and 1.3–2.57 times increased under light cultivation regime of 2.1 variant. The analysis of water balance of plants in vitro cultivated on mannitol under light conditions of 2.1 variant and the plants in conditions of natural growth doesn’t show any considerable distinctions in values of transpiration intensity, water deficit, general water content. The process of adaptation of plants in vitro to conditions ex vitro is accompanied by a change of proline content in leaves with dependence on water deficit in the substrate and light conditions of growing. Conclusions. The obtained results point to the expediency of using free proline content as a biochemical marker for assessing adaptive potential of plants in conditions in vitro and ex vitro.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76828444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. O. Feshina, Z. G. Kucherenko, L. Kovalevska, O. Kashuba
Aim. It is known that in cancerous cells of childhood tumors the pathological changes often include inactivation of the TP53 and RB-E2F1 cellular pathways. One of the proteins controlling the latter pathway is MRPS18-2, that belongs to a family of mitochondrial ribosomal proteins MRPS18. It is important, to study the stability of proteins of this family and their ubiquitination, that might help to conclude about the functional properties of these proteins and their role in cell transformation. Methods. Cloning of cDNA in FLAG vector for expression of fusion proteins, transfection of human tumor cells MCF7, study on cellular localization of MRPS18 family proteins and their ubiquitination by fluorescence microscopy, using specific antibodies. Results. The FLAG-MRPS18-1 and FLAG-MRPS18-3 fusion proteins are partially co-localizing with the HA-Ub fusion protein in the cytoplasm of MCF7 cells. The FLAG-MRPS18-2 protein is localized also in the nucleus. Conclusions. Nuclear localization of the FLAG-MRPS18-2 protein may indicate its additional functions in the cell: due to the interaction with the RB protein and the positive effect on mono-ubiquitination of histone H2B, the MRPS18-2 protein may be involved in the regulation of chromatin structure.
{"title":"Study on ubiquitination of proteins of the MRPS18 family in vitro","authors":"M. O. Feshina, Z. G. Kucherenko, L. Kovalevska, O. Kashuba","doi":"10.7124/feeo.v30.1471","DOIUrl":"https://doi.org/10.7124/feeo.v30.1471","url":null,"abstract":"Aim. It is known that in cancerous cells of childhood tumors the pathological changes often include inactivation of the TP53 and RB-E2F1 cellular pathways. One of the proteins controlling the latter pathway is MRPS18-2, that belongs to a family of mitochondrial ribosomal proteins MRPS18. It is important, to study the stability of proteins of this family and their ubiquitination, that might help to conclude about the functional properties of these proteins and their role in cell transformation. Methods. Cloning of cDNA in FLAG vector for expression of fusion proteins, transfection of human tumor cells MCF7, study on cellular localization of MRPS18 family proteins and their ubiquitination by fluorescence microscopy, using specific antibodies. Results. The FLAG-MRPS18-1 and FLAG-MRPS18-3 fusion proteins are partially co-localizing with the HA-Ub fusion protein in the cytoplasm of MCF7 cells. The FLAG-MRPS18-2 protein is localized also in the nucleus. Conclusions. Nuclear localization of the FLAG-MRPS18-2 protein may indicate its additional functions in the cell: due to the interaction with the RB protein and the positive effect on mono-ubiquitination of histone H2B, the MRPS18-2 protein may be involved in the regulation of chromatin structure.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74174639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Shamilov, A. Abdullayev, V. E. Shamilli, I. Azizov
Aim. The aim of the research was to obtain the zinc complex hypoxanthine-9-riboside and to study its effect during γ-irradiation on the biosynthesis of chlorophylls, carotenoids and on the release of chromosome aberrations in anaphase root hair cells in wheat seedlings. Methods. The zinc complex was obtained by direct interaction of zinc chloride – ZnCl2 with hypoxanthine-9-riboside. X-ray phase analysis and thermogravimetric measurements of the obtained complex were carried out. Before irradiation, seeds of durum wheat Triticum durum L. from a 60Co source were treated with a zinc complex with hypoxanthine-9-riboside at concentrations of 0.1; 0.01; 0.001%. Structural changes in chromosomes were determined in the initial and final stages of anaphase. Determination of chlorophylls and carotenoids was carried out according to Shlyk. Results. g-irradiation at doses of 50, 100 and 200 Gy has a significant effect on the content of green pigments and carotenoids in wheat seedlings. Under the action of g-irradiation, the content of chlorophyll decreases more than carotenoids. With an increase in the dose of radiation, a slight increase in the content of carotenoids is noted. In all variants, chromosomal abnormalities were found: the formation of fragments in metaphase and anaphase, bridges in anaphase, chromosome delays, uneven division of chromosomes. Conclusions. For the first time, it was found that the Zn (II) complex of hypoxanthine-9-riboside at the indicated concentrations significantly reduces the damaging effect of γ-irradiation, helps to eliminate abnormalities in mitotic division in wheat root hair cells.
的目标。本研究的目的是获得锌络合物次黄嘌呤-9-核苷，并研究其在γ辐照下对小麦幼苗后期根毛细胞叶绿素、类胡萝卜素生物合成及染色体畸变释放的影响。方法。氯化锌- ZnCl2与次黄嘌呤-9-核苷直接反应得到锌配合物。对所得配合物进行了x射线物相分析和热重测量。辐照前，用浓度为0.1的次黄嘌呤-9-核苷锌络合物处理来自60Co源的硬粒小麦(Triticum durum L.)种子;0.01;0.001%。染色体的结构变化是在后期的初始和最后阶段确定的。按Shlyk法测定叶绿素和类胡萝卜素。结果。50、100和200 Gy剂量的g辐照对小麦幼苗中绿色色素和类胡萝卜素含量有显著影响。在g辐照作用下，叶绿素含量下降幅度大于类胡萝卜素。随着辐射剂量的增加，类胡萝卜素的含量略有增加。在所有变异中均发现染色体异常:中期和后期形成片段，后期出现桥接，染色体延迟，染色体分裂不均匀。结论。首次发现，在指定浓度下，次黄嘌呤-9-核苷的Zn (II)配合物可显著降低γ辐照的损伤作用，有助于消除小麦根毛细胞有丝分裂的异常。
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