Aim. To define the phenomenon of personal formation and reveal sources of creative energy, and scientific achievements of the Doctor of Biological Sciences, Professor, Corresponding Member of NAS of Ukraine, the Honored Worker of Culture of Ukraine, Laureate of the Ukrainian State Award in the field of architecture, holder of all three classes of the Order “For Merit” and many state, departmental and international awards, Honorary Citizen of Uman, an outstanding scientist and phytobiologist, a world-renowned researcher of hazelnut and the author of hazelnut cultivars Ivan Semenovych Kosenko. He was also a head of the National Dendrological Park “Sofiyivka” of the National Academy of Sciences of Ukraine for 42 years. Methods. Biographical paradigmatic-synergetic methods were used to work, and the cognitive possibilities of the synergetic approach to study the way of life and personality of a scientist in his individuality and imagery in natural disasters and sociopolitical upheavals of the 20th – early 21st centuries. Results. The dynamics of the notion of “personality” and the place of a scientist's personality in scientific cognition in different countries and at different epochs of human development are analyzed. The Curriculum Vitae is presented based on Ivan Semenovych Kosenko's questionnaires and biographical publications of his colleagues, which provide information about his childhood and youth and the role of family and teachers in shaping his personality. Ivan Semenovych's activity during major political and economic crises and natural disasters, when the fate of “Sofiyivka” was under the decision, characterizes him as an effective manager. He was able to assess external and internal threats realistically and successfully overcome the most significant obstacles involving the public, business, and power-holding structures. In the history of world management of preservation, restoration and development of botanical gardens and parks-monuments of landscape art were gone his following achievements: results of restoration of “Sofiyivka” from the consequences of mudflow with more than 70-cm ice, which in night from 3 to 4 April 1980 swept through the park destroying everything on its way; withdrawal in May 1987 of the chemical threat to “Sofiyivka” and the whole Uman district from the possible construction of a nicotine acid plant near Uman; organization of works in the new territories of the park in the turbulent 1990s, when almost all production and construction in the state stopped; restoration of the original appearance of some park landscapes in the historical part of the park and development of new territories in 2014–21; the recruitment original landscapes in the historical part of the park and the restoration of new areas of the park, when the foreign and domestic political situation in Ukraine worsened, accompanied by a drop in the purchasing power of the domestic population and fears of foreign tourists about possible terrorist attack
{"title":"Ivan Semenovych Kosenko – a person, scientist, and citizen","authors":"A. Opalko, V. Kunakh, V. Hrabovyi","doi":"10.7124/feeo.v31.1477","DOIUrl":"https://doi.org/10.7124/feeo.v31.1477","url":null,"abstract":"Aim. To define the phenomenon of personal formation and reveal sources of creative energy, and scientific achievements of the Doctor of Biological Sciences, Professor, Corresponding Member of NAS of Ukraine, the Honored Worker of Culture of Ukraine, Laureate of the Ukrainian State Award in the field of architecture, holder of all three classes of the Order “For Merit” and many state, departmental and international awards, Honorary Citizen of Uman, an outstanding scientist and phytobiologist, a world-renowned researcher of hazelnut and the author of hazelnut cultivars Ivan Semenovych Kosenko. He was also a head of the National Dendrological Park “Sofiyivka” of the National Academy of Sciences of Ukraine for 42 years. Methods. Biographical paradigmatic-synergetic methods were used to work, and the cognitive possibilities of the synergetic approach to study the way of life and personality of a scientist in his individuality and imagery in natural disasters and sociopolitical upheavals of the 20th – early 21st centuries. Results. The dynamics of the notion of “personality” and the place of a scientist's personality in scientific cognition in different countries and at different epochs of human development are analyzed. The Curriculum Vitae is presented based on Ivan Semenovych Kosenko's questionnaires and biographical publications of his colleagues, which provide information about his childhood and youth and the role of family and teachers in shaping his personality. Ivan Semenovych's activity during major political and economic crises and natural disasters, when the fate of “Sofiyivka” was under the decision, characterizes him as an effective manager. He was able to assess external and internal threats realistically and successfully overcome the most significant obstacles involving the public, business, and power-holding structures. In the history of world management of preservation, restoration and development of botanical gardens and parks-monuments of landscape art were gone his following achievements: results of restoration of “Sofiyivka” from the consequences of mudflow with more than 70-cm ice, which in night from 3 to 4 April 1980 swept through the park destroying everything on its way; withdrawal in May 1987 of the chemical threat to “Sofiyivka” and the whole Uman district from the possible construction of a nicotine acid plant near Uman; organization of works in the new territories of the park in the turbulent 1990s, when almost all production and construction in the state stopped; restoration of the original appearance of some park landscapes in the historical part of the park and development of new territories in 2014–21; the recruitment original landscapes in the historical part of the park and the restoration of new areas of the park, when the foreign and domestic political situation in Ukraine worsened, accompanied by a drop in the purchasing power of the domestic population and fears of foreign tourists about possible terrorist attack","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90186847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hao Fu, V. S. Lyutenko, V. Zhmurko, R. Bohuslavskyi
Aim. On the basis of multivariate analysis, to differentiate einkorn wheat accessions by the water-holding capacity of leaves and ears and the parameters of these organs at the level of species and genotypes. Methods. The water-holding capacity was assessed by the moisture-yielding coefficient which was determined by the method of N.N. Kozhushko (Kozhushko, 1988). Differentiation of einkorn samples was carried out by the method of principal components in the interpretation of A.V. Korosov (Korosov, 1996). Results. Triticum monococcum UA0300113, Syria, and UA0300282, Hungary were characterized by the lowest specific moisture-yielding coefficients: for the second and flag leaves, respectively, (mg / cm2) 10.9, 7.0 and 11.4, 10.7; ears – the same samples as well as Triticum boeoticum UA0300401, Ukraine-Crimea: (mg / cm) 29.6, 28.2, 25.4. With an increase in the size of the lamina, there is a tendency to a decrease in its specific moisture yield. The samples of eincorn are differentiated into 4 clusters corresponding to the species. Conclusions. T. monococcum UA0300113, Syria, and UA0300282, Hungary are relatively drought tolerant. The complex of features characterizing leaf and spike size in combination with the moisture-yielding coefficients processed by the method of principal components makes it possible to differentiate einkorn oaccessions by species and genotype. Keywords: einkorns, moisture yield, drought resistance, leaf, ear.
{"title":"Differentiation of eiincorns by water retention capacity","authors":"Hao Fu, V. S. Lyutenko, V. Zhmurko, R. Bohuslavskyi","doi":"10.7124/feeo.v29.1418","DOIUrl":"https://doi.org/10.7124/feeo.v29.1418","url":null,"abstract":"Aim. On the basis of multivariate analysis, to differentiate einkorn wheat accessions by the water-holding capacity of leaves and ears and the parameters of these organs at the level of species and genotypes. Methods. The water-holding capacity was assessed by the moisture-yielding coefficient which was determined by the method of N.N. Kozhushko (Kozhushko, 1988). Differentiation of einkorn samples was carried out by the method of principal components in the interpretation of A.V. Korosov (Korosov, 1996). Results. Triticum monococcum UA0300113, Syria, and UA0300282, Hungary were characterized by the lowest specific moisture-yielding coefficients: for the second and flag leaves, respectively, (mg / cm2) 10.9, 7.0 and 11.4, 10.7; ears – the same samples as well as Triticum boeoticum UA0300401, Ukraine-Crimea: (mg / cm) 29.6, 28.2, 25.4. With an increase in the size of the lamina, there is a tendency to a decrease in its specific moisture yield. The samples of eincorn are differentiated into 4 clusters corresponding to the species. Conclusions. T. monococcum UA0300113, Syria, and UA0300282, Hungary are relatively drought tolerant. The complex of features characterizing leaf and spike size in combination with the moisture-yielding coefficients processed by the method of principal components makes it possible to differentiate einkorn oaccessions by species and genotype. \u0000Keywords: einkorns, moisture yield, drought resistance, leaf, ear.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75252507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. The goal of this work was to study the effect of drought in the critical phase of ontogenesis on the growth and productivity of breed winter wheat plants (Triticum aestivum L.). Methods. Wheat plants of cultivars Darunok Podillya and Podolyanka were grown under optimal nutrition and well-water conditions until the earing-flowering phase, after that the experimental plants were transferred to drought regime for 8 days, after that the optimal water supply was restored to the end of vegetation. During the experiment, the leaf surface area, the mass of internodes and ear were determined. Ripened plants were analyzed according to the structure of the yield. Results. It was established that the effect of drought in the critical phase of ontogenesis the earing-flowering caused to the decreasing in leaf surface area, mass of internodes, ear, mass and number of grains in the ear and plant, weight of 1000 grains. Conclusions. Water deficit in the soil in the critical phase of earing-flowering led to a decrease in the productive of breed winter wheat plants due to the inhibition of growth of ear, decreasing grain number and the mass of ear and grains. Keywords: Triticum aestivum L., internode, ear, productivity, drought.
{"title":"Growth and productivity of wheat plants under drought in the critical phase of ontogenesis","authors":"O. Zhuk, O. Stasik","doi":"10.7124/feeo.v29.1403","DOIUrl":"https://doi.org/10.7124/feeo.v29.1403","url":null,"abstract":"Aim. The goal of this work was to study the effect of drought in the critical phase of ontogenesis on the growth and productivity of breed winter wheat plants (Triticum aestivum L.). Methods. Wheat plants of cultivars Darunok Podillya and Podolyanka were grown under optimal nutrition and well-water conditions until the earing-flowering phase, after that the experimental plants were transferred to drought regime for 8 days, after that the optimal water supply was restored to the end of vegetation. During the experiment, the leaf surface area, the mass of internodes and ear were determined. Ripened plants were analyzed according to the structure of the yield. Results. It was established that the effect of drought in the critical phase of ontogenesis the earing-flowering caused to the decreasing in leaf surface area, mass of internodes, ear, mass and number of grains in the ear and plant, weight of 1000 grains. Conclusions. Water deficit in the soil in the critical phase of earing-flowering led to a decrease in the productive of breed winter wheat plants due to the inhibition of growth of ear, decreasing grain number and the mass of ear and grains. \u0000Keywords: Triticum aestivum L., internode, ear, productivity, drought.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85186615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article covers the life and scientific achievements of an outstanding scientist – geneticist, breeder, botanist, academician, director of the Institute of Botany in 1939–1944, director of the Botanical Garden of the USSR Academy of Sciences in 1944–1958 Mykola Mykolayovych Gryshko (6.01.1901–3.01.1964). Currently, the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine is a leading scientific institution, which includes eight scientific departments and two laboratories with more than 170 researchers. The Botanical Garden on an area of 129.8 hectares has a collection of plants numbering more than 16 thousand taxa. Botanical Garden scientists have developed theoretical and applied principles of introduction, acclimatization, selection, conservation and enrichment of biodiversity. The selection work initiated by M.M. Gryshko successfully continues. About 400 plant cultivars created in the M.M. Gryshko National Botanical Garden are included in the State Register of Plant Varieties of Ukraine. Keywords: anniversary, M. M. Gryshko, academician, geneticist, breeder, director, Botanical Garden.
{"title":"To the 120th anniversary of the birth of academician M.M. Gryshko – an outstanding scientist-genetician, builder of the National botanical garden of the NAS of Ukraine","authors":"S. Klymenko, N. Chuvikina","doi":"10.7124/feeo.v29.1398","DOIUrl":"https://doi.org/10.7124/feeo.v29.1398","url":null,"abstract":"The article covers the life and scientific achievements of an outstanding scientist – geneticist, breeder, botanist, academician, director of the Institute of Botany in 1939–1944, director of the Botanical Garden of the USSR Academy of Sciences in 1944–1958 Mykola Mykolayovych Gryshko (6.01.1901–3.01.1964). Currently, the M.M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine is a leading scientific institution, which includes eight scientific departments and two laboratories with more than 170 researchers. The Botanical Garden on an area of 129.8 hectares has a collection of plants numbering more than 16 thousand taxa. Botanical Garden scientists have developed theoretical and applied principles of introduction, acclimatization, selection, conservation and enrichment of biodiversity. The selection work initiated by M.M. Gryshko successfully continues. About 400 plant cultivars created in the M.M. Gryshko National Botanical Garden are included in the State Register of Plant Varieties of Ukraine. \u0000Keywords: anniversary, M. M. Gryshko, academician, geneticist, breeder, director, Botanical Garden.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80383873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Kozub, I. Sozinov, H. Bidnyk, N. O. Demianova, O. Sozinova, L. Yanse, A. Karelov, Y. Blume
Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.
{"title":"Grain quality indices in common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis.","authors":"N. Kozub, I. Sozinov, H. Bidnyk, N. O. Demianova, O. Sozinova, L. Yanse, A. Karelov, Y. Blume","doi":"10.7124/feeo.v29.1412","DOIUrl":"https://doi.org/10.7124/feeo.v29.1412","url":null,"abstract":"Aim. The aim of this study was to analyze grain quality indices in winter common wheat lines with introgressions of chromosome 1U from Aegilops biuncialis Vis. marked by storage protein loci. Methods. Acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins were performed to identify introgressions. Grain quality indices (SDS sedimentation volume and grain protein content) were analyzed in lines with introgressed chromosome 1U or its arm 1UL, as well as in the cultivars Panna and Bezostaya 1. Results. SDS-sedimentation volume in the cultivars and lines depended on year’s conditions. The studying of the lines during two years has demonstrated that the presence of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis was associated with a high volume of SDS-sedimentation SDS30 (higher than that in the cultivar Bezostaya 1). The introgressive lines show high grain protein content. Conclusions. The effect of the allele at the high molecular weight glutenin subunit locus Glu-U1 from Ae. biuncialis on SDS-sedimentation value is similar to that of the high-quality allele Glu-B1al. The lines with the introgressed allele at Glu-U1 from Ae. biuncialis are valuable initial material for breeding for quality.Keywords: Triticum aestivum, Aegilops biuncialis, high molecular weight glutenin subunits, SDS-sedimentation, protein content.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83372755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim. assessment of accessions from the lentil national collectionon the level of drought resistance on a PEG-6000 solution. Methods. The germination was carried out in growth plates on a filter paper bed at t = 21ºC for five days. A working solution of PEG-6000 with concentrations of 15.0% and 19.5% was used. The control was germinated in distilled water. The length of the shoot and root was measured and the level of depression of growth processes was calculated. Results. When germinating on a PEG-6000 solution with 19.5% concentration, the overwhelming number of accessions had depression indicators for shoots above 85% and above 60% for roots, and therefore it was not possible to isolate samples tolerant to the action of PEG-6000. When germinating on a 15% solution of PEG-6000, a wider range of variation was noted: depression in feature of “root length” ranged from 0% to 100%, “shoot length” - from 50% to 100%. The calculated Spearman's rank correlation coefficient confirms that shoots, in comparison with roots, are more sensitive to the action of osmotic in both variants of the experiment. Conclusions. The concentration of PEG-6000 was established for the selection of sources and the ranking of samples by drought resistance. Sources of drought resistance were identified from the studied sample – accessions subsp. microsperma: 8-MIL M 5 (UD0600007, France), UD0600028 (Ethiopia), Giza 9 (UD0600065, Egypt) and the accession-standard of drought resistance is Gornostepnaya (UD0600131, Armenia) subsp. microsperma. Keywords: lentil, PEG-6000, concentration, sources of drought resistance.
{"title":"Differentiation of accessions from the national lentil collection according to the level of drought resistance on a various concentrations PEG-6000 solution","authors":"N. Vus, A. О. Vasylenko, L. Shevchenko","doi":"10.7124/feeo.v29.1409","DOIUrl":"https://doi.org/10.7124/feeo.v29.1409","url":null,"abstract":"Aim. assessment of accessions from the lentil national collectionon the level of drought resistance on a PEG-6000 solution. Methods. The germination was carried out in growth plates on a filter paper bed at t = 21ºC for five days. A working solution of PEG-6000 with concentrations of 15.0% and 19.5% was used. The control was germinated in distilled water. The length of the shoot and root was measured and the level of depression of growth processes was calculated. Results. When germinating on a PEG-6000 solution with 19.5% concentration, the overwhelming number of accessions had depression indicators for shoots above 85% and above 60% for roots, and therefore it was not possible to isolate samples tolerant to the action of PEG-6000. When germinating on a 15% solution of PEG-6000, a wider range of variation was noted: depression in feature of “root length” ranged from 0% to 100%, “shoot length” - from 50% to 100%. The calculated Spearman's rank correlation coefficient confirms that shoots, in comparison with roots, are more sensitive to the action of osmotic in both variants of the experiment. Conclusions. The concentration of PEG-6000 was established for the selection of sources and the ranking of samples by drought resistance. Sources of drought resistance were identified from the studied sample – accessions subsp. microsperma: 8-MIL M 5 (UD0600007, France), UD0600028 (Ethiopia), Giza 9 (UD0600065, Egypt) and the accession-standard of drought resistance is Gornostepnaya (UD0600131, Armenia) subsp. microsperma. \u0000Keywords: lentil, PEG-6000, concentration, sources of drought resistance.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82551783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Motsnyi, O. Molodchenkova, V. N. Bezlyudnyĭ, M. Litvinenko, Ye. А. Holub, J. S. Fanin
Aim. To determine the value of breeding traits from new sources by studying the resistance to diseases, protein content and their relationship with the productivity of introgression wheat lines. Methods. Growing plants under infection backgrounds of wheat diseases was combined with the laboratory methods for determining protein content and weight of 1000 kernels. Results. Disease resistance has depended on the pathogen species and the source of alien variability. Prolonged resistance to stem rust has been observed only among the derivatives of the collection sample H74/90-245, containing the translocation 1BL.1RS in the karyotype and T. timopheevii in the pedigree. Derivatives Ae. tauschii gradually lost the resistance to the time of full maturation. Conclusions. The parameters of plant resistance to diseases, protein content and yield, absolute protein content in 1000 kernels should be used for the material selection. The best lines have been selected from 736 introgression lines. They characterized by high productivity in some years, large grain, high protein content, disease resistance, adaptability to local environments and devoid of the wild species negative qualities. The lines are of interest for further breeding work in the south of Ukraine. Lines with leaf pubescence from T. timopheevii and with modified translocation 1BL.1RSm were characterized by low productivity.Keywords: wheat, introgression lines, disease resistance, protein content, productivity.
{"title":"Assessment of introgression wheat lines for yield, protein yield and resistance to diseases","authors":"I. Motsnyi, O. Molodchenkova, V. N. Bezlyudnyĭ, M. Litvinenko, Ye. А. Holub, J. S. Fanin","doi":"10.7124/feeo.v29.1414","DOIUrl":"https://doi.org/10.7124/feeo.v29.1414","url":null,"abstract":"Aim. To determine the value of breeding traits from new sources by studying the resistance to diseases, protein content and their relationship with the productivity of introgression wheat lines. Methods. Growing plants under infection backgrounds of wheat diseases was combined with the laboratory methods for determining protein content and weight of 1000 kernels. Results. Disease resistance has depended on the pathogen species and the source of alien variability. Prolonged resistance to stem rust has been observed only among the derivatives of the collection sample H74/90-245, containing the translocation 1BL.1RS in the karyotype and T. timopheevii in the pedigree. Derivatives Ae. tauschii gradually lost the resistance to the time of full maturation. Conclusions. The parameters of plant resistance to diseases, protein content and yield, absolute protein content in 1000 kernels should be used for the material selection. The best lines have been selected from 736 introgression lines. They characterized by high productivity in some years, large grain, high protein content, disease resistance, adaptability to local environments and devoid of the wild species negative qualities. The lines are of interest for further breeding work in the south of Ukraine. Lines with leaf pubescence from T. timopheevii and with modified translocation 1BL.1RSm were characterized by low productivity.Keywords: wheat, introgression lines, disease resistance, protein content, productivity.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79164244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. V. Rayevsky, O. M. Demchyk, P. Karpov, S. Ozheredov, S. Spivak, A. Yemets, Y. Blume
Aim. Search for new dinitroaniline and phosphorothioamide compounds, capable of selective binding with Plasmodium α-tubulin, affecting its mitotic apparatus. Methods. Structural biology methods of computational prediction of protein-ligand interaction: molecular docking, molecular dynamics and pharmacophore analysis. Selection of compounds based on pharmacophore characteristics and virtual screening results. Results. The protocol and required structural conditions for target (α-tubulin of P. falciparum) preparation and correct modeling of the ligand-protein interaction (docking and virtual screening) were developed. The generalized pharmacophore model of ligand-protein interaction and key functional groups of ligands responsible for specific binding were identified. Conclusions. Based on results of virtual screening, 22 commercial compounds were selected. Identified compounds proposed as potential inhibitors of Plasmodium mitotic machinery and the base of new antimalarial drugs. Keywords: malaria, Plasmodium, intermolecular interaction, dinitroaniline derived, phosphorothioamidate derived.
{"title":"Structure-based virtual screening for new lead compounds targeted Plasmodium α-tubulin","authors":"O. V. Rayevsky, O. M. Demchyk, P. Karpov, S. Ozheredov, S. Spivak, A. Yemets, Y. Blume","doi":"10.7124/feeo.v28.1389","DOIUrl":"https://doi.org/10.7124/feeo.v28.1389","url":null,"abstract":"Aim. Search for new dinitroaniline and phosphorothioamide compounds, capable of selective binding with Plasmodium α-tubulin, affecting its mitotic apparatus. Methods. Structural biology methods of computational prediction of protein-ligand interaction: molecular docking, molecular dynamics and pharmacophore analysis. Selection of compounds based on pharmacophore characteristics and virtual screening results. Results. The protocol and required structural conditions for target (α-tubulin of P. falciparum) preparation and correct modeling of the ligand-protein interaction (docking and virtual screening) were developed. The generalized pharmacophore model of ligand-protein interaction and key functional groups of ligands responsible for specific binding were identified. Conclusions. Based on results of virtual screening, 22 commercial compounds were selected. Identified compounds proposed as potential inhibitors of Plasmodium mitotic machinery and the base of new antimalarial drugs. \u0000Keywords: malaria, Plasmodium, intermolecular interaction, dinitroaniline derived, phosphorothioamidate derived.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"75 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91467916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Vozhegova, Y. Lavrynenko, T. Marchenko, V. V. Bazaliĭ
Aim. To establish the manifestation and variability of the trait "plant height" in the lines-parent components and hybrids of corn using different genetic plasmas, to determine the level of heterosis in newly created test crosses and to determine the influence of morphometric parameters on grain yield under irrigation. Methods. General scientific, special, comparative-analytical, regression, information-logical analysis and mathematical modeling. Results. The manifestation and variability of traits "plant height" and "height of cob attachment" in the lines - parent components and hybrids of corn with the use of different genetic plasmas were established. The level of heterosis in newly created test crosses under irrigation conditions was determined. The dependences of biometric parameters and their correlation with grain yield in the test of hybrids obtained with the participation of newly created initial forms of different embryonic plasmas were established. Conclusions. The ratio of plant height of hybrids by maturity groups and yield level showed that for the middle-early group, in the phase of cessation of linear growth, the optimal plant height is 235–265 cm, grain yield is 12.98–13.81 t/ha; for the middle-ripe group - 255–257 cm with grain yield at the level of 15.17–15.82 t/ha. For medium-late hybrids, the optimal plant height to ensure the highest grain yield (over 15 t/ha) is in the range from 270 to 280 cm. Keywords: parental lines, genetic plasmas, hybrids, maize.
{"title":"Manifestation and variability of biometric characteristics in line-parental components and hybrids of maize using different genetic peninsular","authors":"R. Vozhegova, Y. Lavrynenko, T. Marchenko, V. V. Bazaliĭ","doi":"10.7124/feeo.v29.1402","DOIUrl":"https://doi.org/10.7124/feeo.v29.1402","url":null,"abstract":"Aim. To establish the manifestation and variability of the trait \"plant height\" in the lines-parent components and hybrids of corn using different genetic plasmas, to determine the level of heterosis in newly created test crosses and to determine the influence of morphometric parameters on grain yield under irrigation. Methods. General scientific, special, comparative-analytical, regression, information-logical analysis and mathematical modeling. Results. The manifestation and variability of traits \"plant height\" and \"height of cob attachment\" in the lines - parent components and hybrids of corn with the use of different genetic plasmas were established. The level of heterosis in newly created test crosses under irrigation conditions was determined. The dependences of biometric parameters and their correlation with grain yield in the test of hybrids obtained with the participation of newly created initial forms of different embryonic plasmas were established. Conclusions. The ratio of plant height of hybrids by maturity groups and yield level showed that for the middle-early group, in the phase of cessation of linear growth, the optimal plant height is 235–265 cm, grain yield is 12.98–13.81 t/ha; for the middle-ripe group - 255–257 cm with grain yield at the level of 15.17–15.82 t/ha. For medium-late hybrids, the optimal plant height to ensure the highest grain yield (over 15 t/ha) is in the range from 270 to 280 cm. \u0000Keywords: parental lines, genetic plasmas, hybrids, maize.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87115623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Zambriborshch, O. Shestopal, T. Nargan, M. Chekalova
Aim. Testing the haploproduction ability of 30 hybrids of winter soft wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The microspores of 17 of 30 hybrids formed callus by in vitro anther culture were shown. The intensity of one process was different: more than half of the genotypes (18 pcs.) were characterized by a low percentage of callus (from 0.10 to 1.0%), 6 genotypes - medium (from 1.0 to 3.0%), and three - high (4.36%; 15.11% and 15.81%, respectively). Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed Samples P26 and P27 showed the highest level of callus formation. The 10 green regenerating plants were obtained. Keywords: hybrids, soft winter wheat, anther culture in vitro, callus, regeneration.
{"title":"Stabilization of soft wheat selection material which is the result of crossing with wild relatives","authors":"I. Zambriborshch, O. Shestopal, T. Nargan, M. Chekalova","doi":"10.7124/feeo.v28.1380","DOIUrl":"https://doi.org/10.7124/feeo.v28.1380","url":null,"abstract":"Aim. Testing the haploproduction ability of 30 hybrids of winter soft wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The microspores of 17 of 30 hybrids formed callus by in vitro anther culture were shown. The intensity of one process was different: more than half of the genotypes (18 pcs.) were characterized by a low percentage of callus (from 0.10 to 1.0%), 6 genotypes - medium (from 1.0 to 3.0%), and three - high (4.36%; 15.11% and 15.81%, respectively). Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed Samples P26 and P27 showed the highest level of callus formation. The 10 green regenerating plants were obtained. \u0000Keywords: hybrids, soft winter wheat, anther culture in vitro, callus, regeneration.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88762936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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