Objective Urine routines inspection was used as a kind of non invasive test method, which was used widely in the biochemical monitoring of athletes.The urine routine was monitored for nearly eight months prior to the 13th National games in this study, to find out the routine changes in urine of Hubei women's freestyle wrestlers during training cycle and pre-match preparation period. Methods The study was conducted on 22 female freestyle wrestlers in Hubei province, with an average age of 22 years, and 2 athletes of national master level, with professional training period of 5 to 12 years. There are 3 athletes of 48 kg, 5 athletes of 53 kg, 5 athletes of 58 kg, 7 athletes of 63 kg, 1 athlete of 69 kg and 1 athlete of 75 kg. The test instrument is Kyoto PU-4210 urine analyzer. Urine test indicators include：GLU,PRO,BIL,URO,PH,S.G.,BLD,KET,NIT,LEU. The urine sampling test method is divided into two parts.Urine sampling time during convalescence training: from the preparation period of the 13th national games to the pre-competition, the test was conducted on an empty stomach in the morning of the second day of rest every week. Namely: the continuous sampling of morning urine from August 2016 to April 2017, taking the middle urine sample at 7:20 every Monday morning, a total of 425 times. Urine sampling time during training period: from the preparation period of the 13th national games to the pre-competition, the test will be conducted immediately after the actual training. During the period from August 2016 to April 2017, samples were taken according to the training arrangement. After the actual training, urine samples were taken from the middle section and 288 person-times were tested in total. Search and sort out relevant literature to understand the research status. Statistical software SPSS19.0 was used for data processing and paired sample t test was used. Results The results showed that the indicators of the ten positive urine test rates in the training period were: BLD 19.8%, S.G. 17.7%, PRO 14.9%, KET 12.8%,URO 4.5%, WBC 3.8%, BIL 3.1%. The indicators of positive morning urine detection rate during convalescence were:PRO 11.1% ,BLD 8.9% ,S.G. 7.6%, KET 4.2% ,URO 1.9%, WBC 3.0% ,BIL 1.2%. The results of ten subjects in training and convalescence were compared, and the indicators of recovery from fast to slow were successively: S.G. 80.4%, PRO 58.1%, BLD 56.1%, KET 48.6%, WBC 36.4%, BIL was 33.3%, URO 30.7% Conclusions According to the analysis of the change of ten monitoring indexes of female freestyle wrestlers, wrestling is a violent antagonistic sport. The features of the event determine that the energy metabolism of wrestling is an anaerobic, aerobic and metabolic mix and alternation, and anaerobic metabolism is the main sport. The characteristics of the event can lead to different positive reactions in the ten items of urine. 1.The PRO, BLA, S.G. and KET should be regarded as the main indexes because of the high positive ration. This s
{"title":"PO-258 Women's Freestyle Wrestling Athletes Prepare for Urine Ten Evaluation Before the National Games","authors":"L. Xiong","doi":"10.14428/EBR.V1I5.11063","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.11063","url":null,"abstract":"Objective Urine routines inspection was used as a kind of non invasive test method, which was used widely in the biochemical monitoring of athletes.The urine routine was monitored for nearly eight months prior to the 13th National games in this study, to find out the routine changes in urine of Hubei women's freestyle wrestlers during training cycle and pre-match preparation period. \u0000Methods The study was conducted on 22 female freestyle wrestlers in Hubei province, with an average age of 22 years, and 2 athletes of national master level, with professional training period of 5 to 12 years. There are 3 athletes of 48 kg, 5 athletes of 53 kg, 5 athletes of 58 kg, 7 athletes of 63 kg, 1 athlete of 69 kg and 1 athlete of 75 kg. The test instrument is Kyoto PU-4210 urine analyzer. Urine test indicators include：GLU,PRO,BIL,URO,PH,S.G.,BLD,KET,NIT,LEU. The urine sampling test method is divided into two parts.Urine sampling time during convalescence training: from the preparation period of the 13th national games to the pre-competition, the test was conducted on an empty stomach in the morning of the second day of rest every week. Namely: the continuous sampling of morning urine from August 2016 to April 2017, taking the middle urine sample at 7:20 every Monday morning, a total of 425 times. Urine sampling time during training period: from the preparation period of the 13th national games to the pre-competition, the test will be conducted immediately after the actual training. During the period from August 2016 to April 2017, samples were taken according to the training arrangement. After the actual training, urine samples were taken from the middle section and 288 person-times were tested in total. Search and sort out relevant literature to understand the research status. Statistical software SPSS19.0 was used for data processing and paired sample t test was used. \u0000Results The results showed that the indicators of the ten positive urine test rates in the training period were: BLD 19.8%, S.G. 17.7%, PRO 14.9%, KET 12.8%,URO 4.5%, WBC 3.8%, BIL 3.1%. The indicators of positive morning urine detection rate during convalescence were:PRO 11.1% ,BLD 8.9% ,S.G. 7.6%, KET 4.2% ,URO 1.9%, WBC 3.0% ,BIL 1.2%. The results of ten subjects in training and convalescence were compared, and the indicators of recovery from fast to slow were successively: S.G. 80.4%, PRO 58.1%, BLD 56.1%, KET 48.6%, WBC 36.4%, BIL was 33.3%, URO 30.7% \u0000Conclusions According to the analysis of the change of ten monitoring indexes of female freestyle wrestlers, wrestling is a violent antagonistic sport. The features of the event determine that the energy metabolism of wrestling is an anaerobic, aerobic and metabolic mix and alternation, and anaerobic metabolism is the main sport. The characteristics of the event can lead to different positive reactions in the ten items of urine. \u00001.The PRO, BLA, S.G. and KET should be regarded as the main indexes because of the high positive ration. This s","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90589637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective The energy supply of daily training of track cycling should be mainly by anaerobic metabolism, which can make the blood buffer capacity facing huge Challenge and being improved. The arterialized capillary blood gas analysis can be a reliable method to evaluate the blood status of base-acid balance, which may reflect the effect of exercise intensity on blood buffer system. The paper validate the blood gas analysis as a reliable method for evaluating the total periodical training load of track cyclists. Methods Five male and five female elite track cyclists performed two phases (four weeks per phase) of training respectively. The content included the track specific, strength and aerobic training. The Borg's Rating of Perceived Exertion (RPE) Scale (0-11) was recorded in the ten minutes after each session and calculated the session RPE (sRPE). The total inertial load (TIL) was calculated by sRPE sum from Monday to Thursday. The arterialized capillary blood gas analysis was performed at 7:00 AM on Monday and Friday. The delta value (ΔPH, ΔPCO2, ΔTCO2, ΔHCO3-, ΔBE, ΔPO2, ΔSO2) were calculated by Friday minus Monday. Pearson's linear correlation was applied to calculate the correlation between TIL and delta value. Independent t test was used to test the differences between two genders. Results There was the moderate correlation between TIL with ΔPH and ΔHCO3- (Correlation Coefficient= 0.712 and 0.642 respectively, P<0.01). But other blood gas indexes didn’t show the obvious relationship with TIL (Correlation Coefficient < 0.5). There was no differences for TIL between male and female (3870.1±788.4 vs. 4130.2±716.7, P>.05). Moreover, ΔHCO3- of male was significant more than female by 95.1% (P<0.01). There were significant correlation between TIL and ΔPH for both male and female (Correlation Coefficient= 0.785 and 0.812 respectively, P<0.01), and between TIL and ΔPH for both male and female (Correlation Coefficient= 0.662 and 0.658 respectively, P<0.01). Conclusions The PH value and bicarbonate radical of blood should be sensitive to the high intensity track cycling training, which can be the valid to evaluate the inertial load. However, gender has no influence on evaluating. The oxygen partial pressure and oxygen saturation of blood can not reflect the training load accurately.
{"title":"PO-037 Validation Of Capillary Blood Gas Analysis For The Assessment Of Training Load In Track Cycling","authors":"Guoqiang Ma, Qi-chun Tang, D. Ni, B. Parsons","doi":"10.14428/ebr.v1i3.10023","DOIUrl":"https://doi.org/10.14428/ebr.v1i3.10023","url":null,"abstract":"Objective The energy supply of daily training of track cycling should be mainly by anaerobic metabolism, which can make the blood buffer capacity facing huge Challenge and being improved. The arterialized capillary blood gas analysis can be a reliable method to evaluate the blood status of base-acid balance, which may reflect the effect of exercise intensity on blood buffer system. The paper validate the blood gas analysis as a reliable method for evaluating the total periodical training load of track cyclists. Methods Five male and five female elite track cyclists performed two phases (four weeks per phase) of training respectively. The content included the track specific, strength and aerobic training. The Borg's Rating of Perceived Exertion (RPE) Scale (0-11) was recorded in the ten minutes after each session and calculated the session RPE (sRPE). The total inertial load (TIL) was calculated by sRPE sum from Monday to Thursday. The arterialized capillary blood gas analysis was performed at 7:00 AM on Monday and Friday. The delta value (ΔPH, ΔPCO2, ΔTCO2, ΔHCO3-, ΔBE, ΔPO2, ΔSO2) were calculated by Friday minus Monday. Pearson's linear correlation was applied to calculate the correlation between TIL and delta value. Independent t test was used to test the differences between two genders. Results There was the moderate correlation between TIL with ΔPH and ΔHCO3- (Correlation Coefficient= 0.712 and 0.642 respectively, P<0.01). But other blood gas indexes didn’t show the obvious relationship with TIL (Correlation Coefficient < 0.5). There was no differences for TIL between male and female (3870.1±788.4 vs. 4130.2±716.7, P>.05). Moreover, ΔHCO3- of male was significant more than female by 95.1% (P<0.01). There were significant correlation between TIL and ΔPH for both male and female (Correlation Coefficient= 0.785 and 0.812 respectively, P<0.01), and between TIL and ΔPH for both male and female (Correlation Coefficient= 0.662 and 0.658 respectively, P<0.01). Conclusions The PH value and bicarbonate radical of blood should be sensitive to the high intensity track cycling training, which can be the valid to evaluate the inertial load. However, gender has no influence on evaluating. The oxygen partial pressure and oxygen saturation of blood can not reflect the training load accurately.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85622734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To investigate the mechanism of resveratrol improving iron overload in liver of mice and its effect on alleviating liver oxidative damage. Methods 40 male Balb/Cj mice, 2 months old, were randomly divided into 4 groups, Quiet control group (group C), sucrose iron group (group I), resveratrol group (group R), resveratrol + sucrose iron group (group IR), 10 in each group. Group IC and group IR were intraperitoneally injected with sucrose iron solution (100mg/5mL) once every two days, 75uL each time; R group and IR group were intragastrically resveratrol (Res), dissolved in 1% DMSO solution, each time 30mg / Kg; Group C and IC group were intragastrically administered with 1% DMSO solution once daily for 30 mg/Kg. After the end of the intervention for 8 weeks, the mice were dissected and taken. Perls' staining was used to observe the distribution of iron in the liver of mice; biochemical kit for serum glucose, serum iron (SI), total iron binding capacity (TIBC), liver iron, liver superoxide dismutase (T-SOD), liver malondialdehyde (MDA), total liver oxidative capacity (T-AOC); enzyme-linked immunosorbent assay (ELISA) for detection of serum ferritin (SF), octahydroxydeoxyguanosine (8-OHdG); Western blot detection of FPN1 protein expression in liver; the expression of Hepcidin in liver was detected by PCR. Results (1) Perls' staining results: 8 weeks of iron sucrose caused a significant increase in iron content in mouse hepatocytes, and Res decreased the amount of iron ions in hepatocytes; (2) Results of iron metabolism index: 8 weeks of sucrose iron had a significant increase in liver iron, SI, TIBC and SF (P<0.01), and Res intervention reduced liver iron (P<0.05) and SF (P<0.01). The content of TIBC (P<0.01) and SI (P<0.05) increased, and the iron supplement and Res interacted with the effects of liver iron, SI and TIBC; (3) Results of glucose metabolism index: iron overload increased glucose level in mice (P<0.01), Res decreased glucose level, iron overload reduced liver glycogen storage in mice (P<0.05), iron supplement and Res, there is no interaction on the effects of glucose metabolism indicators; (4) Oxidation index results: 8 weeks of sucrose iron significantly inhibited liver T-SOD and T-AOC activity (P<0.01), increased liver MDA and 8-OHDG activity (P<0.01), and Res promoted liver T-AOC (P<0.01) and T-SOD activity increased, and the liver 8-OHDG (P<0.01) and liver MDA activity were decreased. The effects of iron supplement and Res on 8-OHDG and T-AOC were interactive (P<0.01); (5) 8 weeks of sucrose iron inhibited the expression of FPN1 protein in the liver of mice, and the intervention of Res could enhance the expression of FPN1 protein; iron supplement promoted the expression of Hepcidin in liver (P<0.01), Res inhibited Hepcidin, iron supplement and Res had no interaction with the expression of FPN1 and Hepcidin in liver. Conclusions 8 weeks of sucrose iron caused iron overload in the liver of mice, and increased oxidative st
{"title":"PO-295 Effect of resveratrol on preventing iron overload in liver of mice","authors":"Lan-ying Li","doi":"10.14428/EBR.V1I5.13303","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.13303","url":null,"abstract":"Objective To investigate the mechanism of resveratrol improving iron overload in liver of mice and its effect on alleviating liver oxidative damage. \u0000Methods 40 male Balb/Cj mice, 2 months old, were randomly divided into 4 groups, Quiet control group (group C), sucrose iron group (group I), resveratrol group (group R), resveratrol + sucrose iron group (group IR), 10 in each group. Group IC and group IR were intraperitoneally injected with sucrose iron solution (100mg/5mL) once every two days, 75uL each time; R group and IR group were intragastrically resveratrol (Res), dissolved in 1% DMSO solution, each time 30mg / Kg; Group C and IC group were intragastrically administered with 1% DMSO solution once daily for 30 mg/Kg. After the end of the intervention for 8 weeks, the mice were dissected and taken. Perls' staining was used to observe the distribution of iron in the liver of mice; biochemical kit for serum glucose, serum iron (SI), total iron binding capacity (TIBC), liver iron, liver superoxide dismutase (T-SOD), liver malondialdehyde (MDA), total liver oxidative capacity (T-AOC); enzyme-linked immunosorbent assay (ELISA) for detection of serum ferritin (SF), octahydroxydeoxyguanosine (8-OHdG); Western blot detection of FPN1 protein expression in liver; the expression of Hepcidin in liver was detected by PCR. \u0000Results (1) Perls' staining results: 8 weeks of iron sucrose caused a significant increase in iron content in mouse hepatocytes, and Res decreased the amount of iron ions in hepatocytes; (2) Results of iron metabolism index: 8 weeks of sucrose iron had a significant increase in liver iron, SI, TIBC and SF (P<0.01), and Res intervention reduced liver iron (P<0.05) and SF (P<0.01). The content of TIBC (P<0.01) and SI (P<0.05) increased, and the iron supplement and Res interacted with the effects of liver iron, SI and TIBC; (3) Results of glucose metabolism index: iron overload increased glucose level in mice (P<0.01), Res decreased glucose level, iron overload reduced liver glycogen storage in mice (P<0.05), iron supplement and Res, there is no interaction on the effects of glucose metabolism indicators; (4) Oxidation index results: 8 weeks of sucrose iron significantly inhibited liver T-SOD and T-AOC activity (P<0.01), increased liver MDA and 8-OHDG activity (P<0.01), and Res promoted liver T-AOC (P<0.01) and T-SOD activity increased, and the liver 8-OHDG (P<0.01) and liver MDA activity were decreased. The effects of iron supplement and Res on 8-OHDG and T-AOC were interactive (P<0.01); (5) 8 weeks of sucrose iron inhibited the expression of FPN1 protein in the liver of mice, and the intervention of Res could enhance the expression of FPN1 protein; iron supplement promoted the expression of Hepcidin in liver (P<0.01), Res inhibited Hepcidin, iron supplement and Res had no interaction with the expression of FPN1 and Hepcidin in liver. \u0000Conclusions 8 weeks of sucrose iron caused iron overload in the liver of mice, and increased oxidative st","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88754131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective With the population of obese adolescents increases dramatically, a series of cardiovascular diseases, especially atherosclerotic, are triggered by obese which seriously threatens the life and health of teenagers. The aim of this study is to investigate the effects of aerobic exercise intervention on the hemodynamics and structure of the common carotid artery in obese adolescents. Methods Forty obese adolescents (18 ± 2years) were randomly assigned into the experimental group (EG; n = 20) and control group (CG; n = 20). EG undertook 12 weeks of aerobic exercise training (AET), CG had not any exercise intervention. The carotid artery of both CG and EG were examined and compared. Carotid artery responses were assessed in both groups. Color doppler ultrasound was used to determine the tube diameter and axial flow of the common carotid before and after exercise intervention. The heart rate, systolic and diastolic blood pressure were simultaneously measured on the left brachial artery by a sphygmomanometer. Results Compared with CG, there were improvements of EG in peripheral resistance (22.90±6.70 VS 29.58±8.71. p＜0.01) and Systolic blood pressure (123.57±7.36 VS 130.25±6.79. p＜0.05) were verified after AET, except diastolic blood pressure. Following AET, blood flow velocity (0.28±0.05 VS 0.21±0.05. p＜0.01) and wall shear stress (6.25±0.90 VS 4.97±1.54. p＜0.05) increased prominently, which were also significant differences only in EG. In contrast, the vascular diameter demonstrated consistently upper compared with CG, but no differences between EG and CG. Conclusions Regular aerobic exercise lasting 12 weeks could effectively change the dynamic parameters of the common carotid artery in obese adolescents, but no changes in arterial diameter. These findings indicated that 12 weeks of aerobic exercise can induce some changes of the common carotid artery blood flow within the circulation function in a short time. But the changing in common carotid arteries structure is needed after a long-term blood flow to the stimulation.
目的随着青少年肥胖人群的急剧增加，肥胖引发的一系列心血管疾病，尤其是动脉粥样硬化，严重威胁着青少年的生命健康。本研究旨在探讨有氧运动干预对肥胖青少年颈总动脉血流动力学和结构的影响。方法40例肥胖青少年(18±2岁)随机分为实验组(EG;n = 20)和对照组(CG;N = 20)。实验组接受12周的有氧运动训练(AET)，对照组不进行任何运动干预。对CG和EG的颈动脉进行检查和比较。评估两组患者颈动脉反应。采用彩色多普勒超声检测运动干预前后颈总动脉管径及轴流。用血压计同时测量左肱动脉心率、收缩压和舒张压。结果与CG组比较，EG组外周阻力明显改善(22.90±6.70 VS 29.58±8.71)。p<0.01)，收缩压(123.57±7.36 VS 130.25±6.79)。p<0.05)，除舒张压外。AET后血流速度(0.28±0.05 VS 0.21±0.05)。p<0.01)和壁面剪应力(6.25±0.90 VS 4.97±1.54)。p<0.05)显著升高，且仅在EG上差异有统计学意义。与CG相比，血管直径持续增大，但EG和CG之间无差异。结论持续12周的定期有氧运动能有效改变肥胖青少年颈总动脉的动态参数，但对动脉内径无明显影响。这些结果表明，12周的有氧运动可以在短时间内引起循环功能内颈总动脉血流量的一些变化。但颈总动脉结构的改变需要经过长期的血流刺激。
{"title":"OR-022 Effects of aerobic exercise on the hemodynamics and structure of the common carotid artery in obese adolescents","authors":"Wenxia Shi, Ling Cao, Xianzhe Xu","doi":"10.14428/EBR.V1I2.9233","DOIUrl":"https://doi.org/10.14428/EBR.V1I2.9233","url":null,"abstract":"Objective With the population of obese adolescents increases dramatically, a series of cardiovascular diseases, especially atherosclerotic, are triggered by obese which seriously threatens the life and health of teenagers. The aim of this study is to investigate the effects of aerobic exercise intervention on the hemodynamics and structure of the common carotid artery in obese adolescents. \u0000Methods Forty obese adolescents (18 ± 2years) were randomly assigned into the experimental group (EG; n = 20) and control group (CG; n = 20). EG undertook 12 weeks of aerobic exercise training (AET), CG had not any exercise intervention. The carotid artery of both CG and EG were examined and compared. Carotid artery responses were assessed in both groups. Color doppler ultrasound was used to determine the tube diameter and axial flow of the common carotid before and after exercise intervention. The heart rate, systolic and diastolic blood pressure were simultaneously measured on the left brachial artery by a sphygmomanometer. \u0000Results Compared with CG, there were improvements of EG in peripheral resistance (22.90±6.70 VS 29.58±8.71. p＜0.01) and Systolic blood pressure (123.57±7.36 VS 130.25±6.79. p＜0.05) were verified after AET, except diastolic blood pressure. Following AET, blood flow velocity (0.28±0.05 VS 0.21±0.05. p＜0.01) and wall shear stress (6.25±0.90 VS 4.97±1.54. p＜0.05) increased prominently, which were also significant differences only in EG. In contrast, the vascular diameter demonstrated consistently upper compared with CG, but no differences between EG and CG. \u0000Conclusions Regular aerobic exercise lasting 12 weeks could effectively change the dynamic parameters of the common carotid artery in obese adolescents, but no changes in arterial diameter. These findings indicated that 12 weeks of aerobic exercise can induce some changes of the common carotid artery blood flow within the circulation function in a short time. But the changing in common carotid arteries structure is needed after a long-term blood flow to the stimulation.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81299425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To observe the effect of one-time high-intensity intermittent training (HIIT) on the white fat browning in obese rats. Methods 12 obese male rats at the age of 19-week were randomly divided into control group (OC group, n=6) and HIIT group (n=6). OC group doesn’t do exercise, and HIIT group finish the once high-intensity intermittent training ( ①Warm up: 70%VO2max, 7min; ②High intensity exercise: 90%VO2max, 3min+ 50%VO2max, 3min, 6 times; ③Convalescence: 70%VO2max, 7min.). The maximal oxygen uptake (VO2max) of HIIT group was tested before training, and the exercise intensity was determined according to the average VO2max. The subcutaneous and testicle white adipose tissue (WAT) and brown adipose tissue (BAT) of the scapula were taken immediately after exercise. The expression of UCP1 mRNA in the WAT and BAT using the fluorescent quantitative PCR. Results Compared with the OC group, the expression of BAT UCP1 mRNA , in the HIIT group was increased, but there was no significant difference (1.40±0.50 vs 1.03±0.27, P>0.05). In both OC and HIIT group, the expression of UCP1 mRNA of the subcutaneous and testicle WAT were too low to test. The results showed that one-time HIIT exercise had no effect on the expression of UCP1 mRNA in WAT. Conclusions The effects of One-time HIIT on the expression of UCP1 mRNA in adipose tissue of obese rats are still not definite.
{"title":"PO-044 Effects of one-time high intensity intermittent training on the expression of UCP1 mRNA in adipose tissue of obese rats","authors":"Yafei Li, Chunyu Liang, Qishu Zhou, Yi Yan","doi":"10.14428/EBR.V1I3.10093","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.10093","url":null,"abstract":"Objective To observe the effect of one-time high-intensity intermittent training (HIIT) on the white fat browning in obese rats. \u0000Methods 12 obese male rats at the age of 19-week were randomly divided into control group (OC group, n=6) and HIIT group (n=6). OC group doesn’t do exercise, and HIIT group finish the once high-intensity intermittent training ( ①Warm up: 70%VO2max, 7min; ②High intensity exercise: 90%VO2max, 3min+ 50%VO2max, 3min, 6 times; ③Convalescence: 70%VO2max, 7min.). The maximal oxygen uptake (VO2max) of HIIT group was tested before training, and the exercise intensity was determined according to the average VO2max. The subcutaneous and testicle white adipose tissue (WAT) and brown adipose tissue (BAT) of the scapula were taken immediately after exercise. The expression of UCP1 mRNA in the WAT and BAT using the fluorescent quantitative PCR. \u0000Results Compared with the OC group, the expression of BAT UCP1 mRNA , in the HIIT group was increased, but there was no significant difference (1.40±0.50 vs 1.03±0.27, P>0.05). In both OC and HIIT group, the expression of UCP1 mRNA of the subcutaneous and testicle WAT were too low to test. The results showed that one-time HIIT exercise had no effect on the expression of UCP1 mRNA in WAT. \u0000Conclusions The effects of One-time HIIT on the expression of UCP1 mRNA in adipose tissue of obese rats are still not definite.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90650439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective As a new training method, KAATSU Training has become a popular training method because of its obvious training effect, short period and high safety. In this paper, the acute and long-term response of the KAATSU downhill walking Training is discussed to explore its effect on muscle growth, and to provide a theoretical basis for the scientific pressurization of athletes, rehabilitation groups and fitness groups. Methods (1) acute effects: 8 healthy male subjects received pressure and non-pressure slow downhill walking training at the same time and in good physical condition. Pressure in the pressure group was 240mmhg, and pressure bands were tied in the non-pressure group but no pressure. The downhill slope is 16%, the speed is 3km/h, and stride slow walk on the treadmill. Forearm venous blood was taken 15 minutes before training and 15 minutes after training. The concentration of growth hormone (GH), insulin (In), cortisol (C), blood testosterone (T), creatine kinase (CK) and UREA in blood samples were tested respectively. The fingertip blood lactate (Lac) was taken 2 minutes after training, and the subjects' subjective fatigue feelings (RPE) were recorded immediately after training. The changes of surface electromyography (EMG) during training were recorded, and changes of heart rate were recorded every 2 minutes. The thigh and calf circumference were measured before and after exercise. (2) long-term effects: 14 healthy male subjects were randomly divided into the experimental group (KAATSU group 240mmhg, n=8) and the control group (no KAATSU group, n=6), with a slope gradient of 16% and a speed of 3km/h. 2 weeks for 10 days from Monday to Friday. The first week of Monday and the second week of Friday is the same as the acute effects test. At other times, the circumference of the thigh and the calf before and after the training were measured, the heart rate and the subjective fatigue feeling immediately after the exercise were recorded. Results (1)The muscle circumference increased immediately after exercise in both groups. The change of thigh circumference after exercise in the KAATSU group was significantly higher than that in the non- KAATSU group (P<0.05). (2) There were no significant changes in growth hormone, insulin, cortisol, testosterone, creatine kinase and blood urea before and after exercise (P>0.05), and no changes in creatine kinase 24 hours after training. (3) The exercise heart rate of the pressure group was slightly higher than that of the control group ,and the surface electromyography of the KAATSU group was significantly higher than that of the non- KAATSU group (P<0.05). (4) There was no significant difference in subjective fatigue and blood lactic acid between the two groups (P>0.05). (5) After two weeks of KAATSU downhill training walking, the muscle circumference of the leg in the KAATSU group was significantly higher than that of the control group (P<0.05). Conclusions (1) One-time downhill walking tr
{"title":"PO-261 The acute and long-term effects of KAATSU downhill walking training on muscle growth","authors":"Jing Sheng","doi":"10.14428/EBR.V1I5.11093","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.11093","url":null,"abstract":"Objective As a new training method, KAATSU Training has become a popular training method because of its obvious training effect, short period and high safety. In this paper, the acute and long-term response of the KAATSU downhill walking Training is discussed to explore its effect on muscle growth, and to provide a theoretical basis for the scientific pressurization of athletes, rehabilitation groups and fitness groups. \u0000Methods (1) acute effects: 8 healthy male subjects received pressure and non-pressure slow downhill walking training at the same time and in good physical condition. Pressure in the pressure group was 240mmhg, and pressure bands were tied in the non-pressure group but no pressure. The downhill slope is 16%, the speed is 3km/h, and stride slow walk on the treadmill. Forearm venous blood was taken 15 minutes before training and 15 minutes after training. The concentration of growth hormone (GH), insulin (In), cortisol (C), blood testosterone (T), creatine kinase (CK) and UREA in blood samples were tested respectively. The fingertip blood lactate (Lac) was taken 2 minutes after training, and the subjects' subjective fatigue feelings (RPE) were recorded immediately after training. The changes of surface electromyography (EMG) during training were recorded, and changes of heart rate were recorded every 2 minutes. The thigh and calf circumference were measured before and after exercise. \u0000(2) long-term effects: 14 healthy male subjects were randomly divided into the experimental group (KAATSU group 240mmhg, n=8) and the control group (no KAATSU group, n=6), with a slope gradient of 16% and a speed of 3km/h. 2 weeks for 10 days from Monday to Friday. The first week of Monday and the second week of Friday is the same as the acute effects test. At other times, the circumference of the thigh and the calf before and after the training were measured, the heart rate and the subjective fatigue feeling immediately after the exercise were recorded. \u0000Results (1)The muscle circumference increased immediately after exercise in both groups. The change of thigh circumference after exercise in the KAATSU group was significantly higher than that in the non- KAATSU group (P<0.05). \u0000(2) There were no significant changes in growth hormone, insulin, cortisol, testosterone, creatine kinase and blood urea before and after exercise (P>0.05), and no changes in creatine kinase 24 hours after training. \u0000(3) The exercise heart rate of the pressure group was slightly higher than that of the control group ,and the surface electromyography of the KAATSU group was significantly higher than that of the non- KAATSU group (P<0.05). \u0000(4) There was no significant difference in subjective fatigue and blood lactic acid between the two groups (P>0.05). \u0000(5) After two weeks of KAATSU downhill training walking, the muscle circumference of the leg in the KAATSU group was significantly higher than that of the control group (P<0.05). \u0000Conclusions (1) One-time downhill walking tr","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90784322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Human muscle consists of a mixture of fibres with different contractile and metabolic properties, type I (slow-twitch) and type II (fast-twitch) fibres. Little is known about the effect of anabolic stimuli, in particular nutrition, on the molecular response in the different fibre types. Here, we examine the effect of resistance exercise and essential amino acid (EAA) supplementation on mTOR signalling in individual type I and type II human muscle fibres. Methods Five strength-trained male subjects performed two sessions of leg press exercise (10 x 10 repetition at 62-85 % of 1RM). During exercise and recovery, the subjects ingested an aqueous solution with EAA (290 mg/kg) or flavoured water (placebo). Muscle biopsies were taken from the vastus lateralis before and 90 min after exercise. The biopsies were freeze-dried and single fibres dissected out and weighed (range 0.9 – 8 ug). The fibres were individually homogenized and analysed for proteins in the mTOR pathway using Western blot. Membranes were repeatedly stripped and fibres were identified as type I or type II following incubation with antibodies against the different myosin isoforms. Results Exercise led to a significant increase in mTOR and p70S6k1 phosphorylation and a fall in eEF2 phosphorylation, similar in both fibre types. There was a large variation between individual fibres; some fibres were highly activated whereas others were not activated at all despite the heavy exercise performed. Intake of EAA caused a 2- to 6-fold higher increase in mTOR and p70S6k1 phosphorylation in both type I and type II fibres as compared to intake of placebo, with no difference between the fibre types. The phosphorylation of eEF2 was not affected by intake of EAA. The total expression of p70S6k1 and eEF2 was 145% and 155% higher in type II than in type I fibres (P<0.05), respectively, whereas no difference between the fibre types was observed for mTOR protein. Conclusions The response to heavy resistance exercise regarding mTOR signalling was similar in type I and type II fibres in trained subjects, but with a large variation between single fibres of both types. Furthermore, ingestion of EAA enhanced the effect of resistance exercise on phosphorylation of mTOR and p70S6k1 in both fibre types.
{"title":"PO-260 Anabolic signalling in individual muscle fibres following resistance exercise in combination with amino acid intake","authors":"S. Edman, K. Söderlund, E. Blomstrand","doi":"10.14428/EBR.V1I5.11083","DOIUrl":"https://doi.org/10.14428/EBR.V1I5.11083","url":null,"abstract":"Objective Human muscle consists of a mixture of fibres with different contractile and metabolic properties, type I (slow-twitch) and type II (fast-twitch) fibres. Little is known about the effect of anabolic stimuli, in particular nutrition, on the molecular response in the different fibre types. Here, we examine the effect of resistance exercise and essential amino acid (EAA) supplementation on mTOR signalling in individual type I and type II human muscle fibres. \u0000Methods Five strength-trained male subjects performed two sessions of leg press exercise (10 x 10 repetition at 62-85 % of 1RM). During exercise and recovery, the subjects ingested an aqueous solution with EAA (290 mg/kg) or flavoured water (placebo). Muscle biopsies were taken from the vastus lateralis before and 90 min after exercise. The biopsies were freeze-dried and single fibres dissected out and weighed (range 0.9 – 8 ug). The fibres were individually homogenized and analysed for proteins in the mTOR pathway using Western blot. Membranes were repeatedly stripped and fibres were identified as type I or type II following incubation with antibodies against the different myosin isoforms. \u0000Results Exercise led to a significant increase in mTOR and p70S6k1 phosphorylation and a fall in eEF2 phosphorylation, similar in both fibre types. There was a large variation between individual fibres; some fibres were highly activated whereas others were not activated at all despite the heavy exercise performed. Intake of EAA caused a 2- to 6-fold higher increase in mTOR and p70S6k1 phosphorylation in both type I and type II fibres as compared to intake of placebo, with no difference between the fibre types. The phosphorylation of eEF2 was not affected by intake of EAA. The total expression of p70S6k1 and eEF2 was 145% and 155% higher in type II than in type I fibres (P<0.05), respectively, whereas no difference between the fibre types was observed for mTOR protein. \u0000Conclusions The response to heavy resistance exercise regarding mTOR signalling was similar in type I and type II fibres in trained subjects, but with a large variation between single fibres of both types. Furthermore, ingestion of EAA enhanced the effect of resistance exercise on phosphorylation of mTOR and p70S6k1 in both fibre types. \u0000 ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89361976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To explore the development and developmental characteristics of forearm bone mineral density in childhood, to understand the effects of body composition and developmental type on bone mineral density, and to provide a basis for studying the changes of human forearm bone mineral density. Methods Bone development follow-up study of Huilongguan primary school affiliated to Capital Normal University in 2013 enrolled in primary school for five years (2013, 2014, 2015, 2016 and 2017), tracking test indicators: height, weight, body fat composition, forearm bone density. Bone age testing was conducted in 2013 and 2017. Data were analyzed for students who were 6 years old at the end of the first year of the test date. The effective data was 297 (age 6.6 ± 0.2 years), including 147 boys and 150 girls. Results 1. Forearm bone mineral density(g/cm2)for the boys and girls were 0.237±0.061 and 0.221±0.059 for children aged 6, 0.220±0.042 and 0.201±0.032 for 7 years old children, 0.219±0.040 and 0.198±0.038 for 8 years old children, 0.236±0.051 and 0.208±0.043 for 9 years old children ,while 0.237±0.044 and 0.213±0.047 when children were 10 years old. An independent sample t-test was performed on bone mineral density in boys and girls. At 7 years, 8 years, 9 years, and 10 years, the bone mineral density of boys was greater than that of girls, and the difference was statistically significant (p<0.05). 2. The same batch of child follow-up studies found that girls and boys aged 6 to 8 had a decreasing trend, and the decline of girls was greater than that of boys; girls and boys aged 8 to 10 have risen again, and the increase in girls is less than that of boys; body weight and BMI are factors influencing the forearm bone density of children aged 6-10 years. Height changes and weight changes have a certain effect on changes in forearm bone density. 3. There was no statistically significant difference between bone mineral density and height. The partial correlation with body weight was statistically significant between 6 and 10 years old, r=0.200 (p<0.01), r=0.124 (p<0.05), r =0.176 (p<0.01), r=0.110 (p<0.05), r=0.162 (p<0.05); the partial correlation with BMI was statistically significant, r=0.223 (p<0.01), r =0.134 (p<0.01), r=0.183 (p<0.01), r=0.150 (p<0.05)r=0.208 (p<0.01); As for the relationships between body fat ratio、FFM ratio and bone mineral density, there are no partial correlations(p＞0.05). 4. The partial correlation between bone mineral density change and height change (6-8 years old) and body weight change (8-10 years old) was statistically significant, r=-0.138 (p<0.05), r=0.178(p <0.01), the change in bone mineral density of the children with highest 25% and the lowest 25% with different indicators, only the height of the independent sample t-test results was statistically significant. 5. After multiple stepwise linear regression model. At the age of 7, the adjustment of R2 is the highest. The gender and body mass index entered the model.
{"title":"PO-091 5-year changes and effects of bone mineral density in 6-year-old children","authors":"Yue Liang, Hong Ren","doi":"10.14428/EBR.V1I3.11653","DOIUrl":"https://doi.org/10.14428/EBR.V1I3.11653","url":null,"abstract":"Objective To explore the development and developmental characteristics of forearm bone mineral density in childhood, to understand the effects of body composition and developmental type on bone mineral density, and to provide a basis for studying the changes of human forearm bone mineral density. \u0000Methods Bone development follow-up study of Huilongguan primary school affiliated to Capital Normal University in 2013 enrolled in primary school for five years (2013, 2014, 2015, 2016 and 2017), tracking test indicators: height, weight, body fat composition, forearm bone density. Bone age testing was conducted in 2013 and 2017. Data were analyzed for students who were 6 years old at the end of the first year of the test date. The effective data was 297 (age 6.6 ± 0.2 years), including 147 boys and 150 girls. \u0000Results 1. Forearm bone mineral density(g/cm2)for the boys and girls were 0.237±0.061 and 0.221±0.059 for children aged 6, 0.220±0.042 and 0.201±0.032 for 7 years old children, 0.219±0.040 and 0.198±0.038 for 8 years old children, 0.236±0.051 and 0.208±0.043 for 9 years old children ,while 0.237±0.044 and 0.213±0.047 when children were 10 years old. An independent sample t-test was performed on bone mineral density in boys and girls. At 7 years, 8 years, 9 years, and 10 years, the bone mineral density of boys was greater than that of girls, and the difference was statistically significant (p<0.05). 2. The same batch of child follow-up studies found that girls and boys aged 6 to 8 had a decreasing trend, and the decline of girls was greater than that of boys; girls and boys aged 8 to 10 have risen again, and the increase in girls is less than that of boys; body weight and BMI are factors influencing the forearm bone density of children aged 6-10 years. Height changes and weight changes have a certain effect on changes in forearm bone density. 3. There was no statistically significant difference between bone mineral density and height. The partial correlation with body weight was statistically significant between 6 and 10 years old, r=0.200 (p<0.01), r=0.124 (p<0.05), r =0.176 (p<0.01), r=0.110 (p<0.05), r=0.162 (p<0.05); the partial correlation with BMI was statistically significant, r=0.223 (p<0.01), r =0.134 (p<0.01), r=0.183 (p<0.01), r=0.150 (p<0.05)r=0.208 (p<0.01); As for the relationships between body fat ratio、FFM ratio and bone mineral density, there are no partial correlations(p＞0.05). 4. The partial correlation between bone mineral density change and height change (6-8 years old) and body weight change (8-10 years old) was statistically significant, r=-0.138 (p<0.05), r=0.178(p <0.01), the change in bone mineral density of the children with highest 25% and the lowest 25% with different indicators, only the height of the independent sample t-test results was statistically significant. 5. After multiple stepwise linear regression model. At the age of 7, the adjustment of R2 is the highest. The gender and body mass index entered the model.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84024495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective AMP-activated protein kinase (AMPK) is a metabolic energy sensor and its activation plays an important role in the regulation of energy homeostasis. Increasing evidence indicates that AMPK activation depend on the phosphorylation sites in AMPKα. Thr172 is involved in AMPK activation, whereas Ser485/491 are not. Under suitable stress stimulations, the phosphorylation of AMPKα at the Thr172 site can increase AMPK activation. However, serious hypoxic exercise or taking antioxidants before exercise can reduce the activation of AMPK by phosphorylating AMPK α1Ser485/α2Ser491 sites. The aim of this study was to investigate the effects of hypoxia preconditioning on exhaustive exercise under hypoxic condition induced AMPKα Thr172 and Ser485/491 phosphorylation in mice skeletal muscle. Methods The 40 eight-week-old male C57BL/6J wild type mice were randomly divided into four groups (10 mice /group): non-hypoxia preconditioning control group (NC), hypoxia preconditioning control group (HC), non-hypoxia preconditioning acute hypoxic exercise group (NE), and hypoxia preconditioning acute hypoxic exercise group (HE). Hypoxia preconditioning groups were exposure in hypoxia for 48h, with the oxygen concentration was 11.2%. Meanwhile, non-hypoxia preconditioning was in the normoxic condition for 48h. After hypoxia preconditioning, acute hypoxic exercise groups finished an exhaustive exercise. Tibialis anterior muscles of mice were collected immediately after the exhaustive exercise. The protein expression of the total AMPKα, Thr172-AMPKα phosphorylation, and Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation were measured by Western Blot. Thr172-AMPKα phosphorylation to total AMPKα ratio and Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation to total AMPKα ratio was calculated. Results Compared with NE group, The Thr172-AMPKα phosphorylation to total AMPKα ratio was increased significantly, whereas the relative expression of Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation to total AMPKα ratio seemed to decreased in skeletal muscle of HE group. Conclusions The 48h hypoxia preconditioning could improve the AMPK activation by Thr172-AMPKα phosphorylation in mice skeletal muscle following an exhaustive exercise under the hypoxic condition.
{"title":"PO-142 Effects of hypoxia preconditioning on acute hypoxic exercise-induced phosphorylation of AMPKα in mice skeletal muscle","authors":"Haotian Wei, Lin Wang, Ying Zhang","doi":"10.14428/EBR.V1I4.10453","DOIUrl":"https://doi.org/10.14428/EBR.V1I4.10453","url":null,"abstract":"Objective AMP-activated protein kinase (AMPK) is a metabolic energy sensor and its activation plays an important role in the regulation of energy homeostasis. Increasing evidence indicates that AMPK activation depend on the phosphorylation sites in AMPKα. Thr172 is involved in AMPK activation, whereas Ser485/491 are not. Under suitable stress stimulations, the phosphorylation of AMPKα at the Thr172 site can increase AMPK activation. However, serious hypoxic exercise or taking antioxidants before exercise can reduce the activation of AMPK by phosphorylating AMPK α1Ser485/α2Ser491 sites. The aim of this study was to investigate the effects of hypoxia preconditioning on exhaustive exercise under hypoxic condition induced AMPKα Thr172 and Ser485/491 phosphorylation in mice skeletal muscle. \u0000Methods The 40 eight-week-old male C57BL/6J wild type mice were randomly divided into four groups (10 mice /group): non-hypoxia preconditioning control group (NC), hypoxia preconditioning control group (HC), non-hypoxia preconditioning acute hypoxic exercise group (NE), and hypoxia preconditioning acute hypoxic exercise group (HE). Hypoxia preconditioning groups were exposure in hypoxia for 48h, with the oxygen concentration was 11.2%. Meanwhile, non-hypoxia preconditioning was in the normoxic condition for 48h. After hypoxia preconditioning, acute hypoxic exercise groups finished an exhaustive exercise. Tibialis anterior muscles of mice were collected immediately after the exhaustive exercise. The protein expression of the total AMPKα, Thr172-AMPKα phosphorylation, and Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation were measured by Western Blot. Thr172-AMPKα phosphorylation to total AMPKα ratio and Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation to total AMPKα ratio was calculated. \u0000Results Compared with NE group, The Thr172-AMPKα phosphorylation to total AMPKα ratio was increased significantly, whereas the relative expression of Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation to total AMPKα ratio seemed to decreased in skeletal muscle of HE group. \u0000Conclusions The 48h hypoxia preconditioning could improve the AMPK activation by Thr172-AMPKα phosphorylation in mice skeletal muscle following an exhaustive exercise under the hypoxic condition.","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87884984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective Peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β) is mainly expressed in mitochondria-rich tissues, which involved in skeletal muscle mitochondrial biosynthesis and energy metabolism processes such as fatty acid transport and oxidation, hepatic gluconeogenesis. PGC-1β is Previous studies have shown that this genetic polymorphism is associated with the athletic ability of elite endurance athletes. Therefore, based on the previous research, the relationship between PGC-1β gene polymorphism and aerobic exercise ability of elite female long-distance runners was discussed to provide new effective indicators for athletes' selection of materials, and improve the accuracy and advancement of athletes' selection of materials. Methods 56 Chinese elite female long-distance runners were selected, and venous blood was extracted to analyze the gene polymorphism of specific gene locus. The subjects were tested for aerobic endurance index and lung function index, wherein the aerobic endurance index included maximum oxygen uptake, relative maximal oxygen uptake relative value, anaerobic threshold and anaerobic threshold relative value, and lung function indicators including vital capacity, Time lung capacity, minute ventilation and maximum ventilation . Subsequently, the cross-sectional association study method was used to analyze the association between four genotypes locus of PGC-1β including rs32579, rs2161257, rs1544744 and rs10783180 in 56 subjects. Results 1) All four polymorphic locus were tested by H-W balance, indicating that the subjects were representative of the population. 2) rs32579 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 3) rs2161257 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 4) rs1544744 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 5) rs10783180 locus: There is a significant difference in the relative values of anaerobic threshold and anaerobic threshold between different genotype athletes. The anaerobic threshold of AG genotype athletes was 2156.35±227.69 ml/min and the anaerobic threshold of athletes with GG genotype was 2143.41±217.30 ml/min. So the anaerobic threshold of AG genotype athletes was significantly greater than the anaerobic threshold of GG genotype athletes. The Anaerobic threshold relative value for AG genotype athletes was 50.99±3.99 ml/kg/min, while the anaerobic threshold relative value for athletes with GG genotype was 48.12±4.25 ml/kg/min. The anaerobic threshold relative value of AG genotype athletes was significantly greater than that of GG genotype athletes Other indicators showed no significant difference. Conclusions The rs10783180 polymorphism is associated with the athletic ability of elite endurance
{"title":"OR-025 Association study between Chinese excellent long-distance female athletes’ PGC-1β genetic polymorphism and aerobic capacity","authors":"J. Jia, Chunyan Xu","doi":"10.14428/EBR.V1I2.9263","DOIUrl":"https://doi.org/10.14428/EBR.V1I2.9263","url":null,"abstract":"Objective Peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β) is mainly expressed in mitochondria-rich tissues, which involved in skeletal muscle mitochondrial biosynthesis and energy metabolism processes such as fatty acid transport and oxidation, hepatic gluconeogenesis. PGC-1β is Previous studies have shown that this genetic polymorphism is associated with the athletic ability of elite endurance athletes. Therefore, based on the previous research, the relationship between PGC-1β gene polymorphism and aerobic exercise ability of elite female long-distance runners was discussed to provide new effective indicators for athletes' selection of materials, and improve the accuracy and advancement of athletes' selection of materials. \u0000Methods 56 Chinese elite female long-distance runners were selected, and venous blood was extracted to analyze the gene polymorphism of specific gene locus. The subjects were tested for aerobic endurance index and lung function index, wherein the aerobic endurance index included maximum oxygen uptake, relative maximal oxygen uptake relative value, anaerobic threshold and anaerobic threshold relative value, and lung function indicators including vital capacity, Time lung capacity, minute ventilation and maximum ventilation . Subsequently, the cross-sectional association study method was used to analyze the association between four genotypes locus of PGC-1β including rs32579, rs2161257, rs1544744 and rs10783180 in 56 subjects. \u0000Results 1) All four polymorphic locus were tested by H-W balance, indicating that the subjects were representative of the population. 2) rs32579 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 3) rs2161257 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 4) rs1544744 locus: There were no significant differences in lung function indicators and aerobic exercise capacity between different genotype athletes. 5) rs10783180 locus: There is a significant difference in the relative values of anaerobic threshold and anaerobic threshold between different genotype athletes. The anaerobic threshold of AG genotype athletes was 2156.35±227.69 ml/min and the anaerobic threshold of athletes with GG genotype was 2143.41±217.30 ml/min. So the anaerobic threshold of AG genotype athletes was significantly greater than the anaerobic threshold of GG genotype athletes. The Anaerobic threshold relative value for AG genotype athletes was 50.99±3.99 ml/kg/min, while the anaerobic threshold relative value for athletes with GG genotype was 48.12±4.25 ml/kg/min. The anaerobic threshold relative value of AG genotype athletes was significantly greater than that of GG genotype athletes \u0000Other indicators showed no significant difference. \u0000Conclusions The rs10783180 polymorphism is associated with the athletic ability of elite endurance ","PeriodicalId":12276,"journal":{"name":"Exercise Biochemistry Review","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88042485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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