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Comprehensive metabolomics and phytochemical analyses identified important metabolites involved in the antioxidant activity of four Swiss chard cultivars (Beta vulgaris L. var. cicla) with different leaf colours 综合代谢组学和植物化学分析鉴定了4种不同叶色瑞士甜菜品种(Beta vulgaris L. var. cicla)抗氧化活性的重要代谢物
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-01 DOI: 10.1016/j.fochx.2026.103587
Chanung Park , Jae Kwang Kim , Jinsu Lim , Kihyun Kim , Haejin Kwon , Eun Sol Cho , Ye Jin Kim , Moon-Sub Lee , Sujatha Ramasamy , Ramaraj Sathasivam , Sang Un Park
Beta vulgaris L. var. cicla is a leaf vegetable and has been used in many cuisines worldwide for a long time. The long history of Swiss chard cultivation has resulted in the development of many cultivars through domestication and hybridisation. The diversification of Swiss chard cultivars has resulted in differences in metabolite profiles. However, comparative studies analysing metabolites among cultivars are still lacking. In this study, 63 metabolites were identified in four Swiss chard cultivars with different leaf petiole colours using HPLC, GC–qMS, and GC–TOFMS. In addition, the antioxidant activity, TPC, and TFC were determined in extracts from the four Swiss chard cultivars. Comprehensive metabolic profiling analysis was performed using metabolites identified in Swiss chard. In addition to flavonoids, the metabolomic data and antioxidant assay identified non-flavonoid metabolites, such as policosanol, carotenoids, and glutamic acid derivatives, as strong antioxidant candidates that were not previously recognized as antioxidants in Swiss chard.
甜菜(Beta vulgaris L. var. cicla)是一种叶菜,长期以来在世界各地的许多菜系中都有使用。瑞士甜菜栽培历史悠久,通过驯化和杂交培育出了许多品种。瑞士甜菜品种的多样化导致了其代谢谱的差异。然而,品种间代谢物的比较研究仍然缺乏。采用HPLC、GC-qMS和GC-TOFMS对4个不同叶柄颜色的瑞士甜菜品种进行了63种代谢物的鉴定。此外,测定了4个品种甜菜提取物的抗氧化活性、TPC和TFC。利用在瑞士甜菜中鉴定的代谢物进行了全面的代谢分析。除了类黄酮外,代谢组学数据和抗氧化分析还确定了非类黄酮代谢物,如胆甾醇、类胡萝卜素和谷氨酸衍生物,作为强抗氧化剂候选物,这些物质在瑞士甜菜中以前没有被认为是抗氧化剂。
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引用次数: 0
Co-fermentation of Pichia kudriavzevii and Millerozyma farinosa modulates fungal community and enhances flavor in Yangjiang douchi 双歧毕赤酵母与粉孢千霉共发酵对阳江豆豉真菌群落的调节及风味的改善
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-01 DOI: 10.1016/j.fochx.2026.103603
Donghui Luo , Xiaoli Jiang , Mengting Zhang , Zilong Zhao , Jianxu Chen , Jian Liang , Jingjing Guan , Huiyan Zhao
Two aromatic yeast strains, YPKJ (Pichia kudriavzevii) and YMFJ (Millerozyma farinosa), were isolated and identified from Yangjiang fermented douchi. The optimal fermentation conditions—9% salt content, 34 °C, 4% inoculum size, and a 1:2 inoculum ratio—were determined based on amino acid nitrogen and total acidity. Using a Co-fermentation strategy combined with MiSeq sequencing and gas chromatography–mass spectrometry (GC–MS), we investigated the influence of microbial communities and flavor compounds during douchi post-fermentation. Results revealed 156 volatile compounds identified by GC–MS, with their content increasing approximately 6.7-fold during ZF fermentation. Specifically, the ester content in Z10 was 1.38 times higher than that in Y30, and the phenethyl alcohol content in Z10 was 2.4 times higher than that in Y20. This study provides a theoretical and technical basis for regulating the microbial community in fermented black bean production and for optimizing industrial manufacturing processes.
从阳江豆豉中分离鉴定了两株芳香酵母菌YPKJ (Pichia kudriavzevii)和YMFJ (Millerozyma farinosa)。以氨基酸氮和总酸度为指标,确定了最佳发酵条件为9%的含盐量、34℃、4%的接种量、1:2的接种比。采用MiSeq测序和气相色谱-质谱(GC-MS)相结合的共发酵策略,研究了豆豉发酵后微生物群落和风味化合物的影响。结果发现,经GC-MS鉴定出的挥发性化合物有156种,在ZF发酵过程中含量增加了约6.7倍。其中,Z10的酯含量是Y30的1.38倍,Z10的苯乙醇含量是Y20的2.4倍。本研究为黑豆发酵过程中微生物群落的调控和工业生产工艺的优化提供了理论和技术依据。
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引用次数: 0
Soy protein isolate-pectin from Nicandra Physaloides (Linn.) Gaertn seeds nanogels: A hydrocolloid system for blueberry preservation 大豆分离蛋白-果胶from Nicandra Physaloides (Linn.)蓝莓种子纳米凝胶:一种用于蓝莓保存的水胶体系统
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-01 DOI: 10.1016/j.fochx.2026.103567
Dan Meng , Wenqi Yang , Zhong Zhang, Xiaoyu Wang, Jianke Li, Li Yuan
Resveratrol (Res) and Phloretin (Pht) are natural compounds with antioxidant and anti-inflammatory activities, yet their practical applications face certain limitations. In this study, nanogels were utilized as carriers to enhance their characteristics and bioactivities. Soy protein isolate (SPI) and pectin (NPGP) derived from Nicandra physaloides (Linn.) Gaertn seeds were successfully prepared using a two-step method involving Maillard dry heat and heated self-assembly. SPI-NPGP nanogels (NGs) of were determined to be 2 mg/mL SPI-NPGP grafts, heat-treated at 90 °C for 40 min at pH = 4.6. The loading efficiency of NGs containing Res and Pht reached 4.03% and 8.15%, respectively, and Res/Pht-NGs were spherical in shape, uniformly distributed, and hydrophilic. Stability studies confirmed their good pH tolerance, storage stability, in vitro stability, along with strong antioxidant activity. Additionally, Res/Pht-NGs showed significant inhibitory effect against Staphylococcus aureus (S. aureus). The application of Res/Pht-NGs in blueberry preservation effectively reduced weight loss and decay incidence by slowing respiratory rates, preserving anthocyanin content, antioxidant capacity, and flavonoid levels, while inhibiting the degradation of volatile organic compounds. In conclusion, Res/Pht-NGs exhibit excellent stability and effective blueberries preservation, offering a novel approach for food preservation and the application of NPGP.
白藜芦醇(Resveratrol, Res)和根皮素(Pht)是具有抗氧化和抗炎活性的天然化合物,但其实际应用存在一定的局限性。在本研究中,纳米凝胶作为载体,以提高其特性和生物活性。大豆分离蛋白(SPI)和果胶(NPGP),分别来源于Nicandra physaloides (Linn.)采用美拉德干热和加热自组装两步法成功制备了栀子籽。SPI-NPGP纳米凝胶(NGs)为2 mg/mL SPI-NPGP接枝,90°C, pH = 4.6,热处理40 min。含Res和Pht的NGs的加载效率分别达到4.03%和8.15%,Res/Pht-NGs呈球形,分布均匀,亲水性好。稳定性研究证实其具有良好的耐pH性、贮存稳定性、体外稳定性以及较强的抗氧化活性。此外,Res/Pht-NGs对金黄色葡萄球菌(S. aureus)有显著的抑制作用。Res/Pht-NGs在蓝莓保鲜中的应用,通过减缓呼吸速率、保持花青素含量、抗氧化能力和类黄酮水平,同时抑制挥发性有机物的降解,有效地减少了蓝莓的失重和腐烂发生率。综上所述,Res/Pht-NGs具有良好的稳定性和有效的蓝莓保鲜效果,为蓝莓食品保鲜和NPGP的应用提供了新的途径。
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引用次数: 0
Differential analysis of free and bound phenolic compounds and its antioxidant activity of Ficus hirta Vahl. root cortex with different leaf morphotypes based on metabolomics 无花果游离和结合酚类化合物的差异分析及其抗氧化活性。基于代谢组学的不同叶型根皮质
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-01 DOI: 10.1016/j.fochx.2026.103554
Qing Gui , Qingmian Chen , Yufeng Zhang , Xiu Zeng , Shiyu Li , Jianxiong Huang , Xiuquan Wang
The free and bound phenolic compounds and its antioxidant activities in the root cortext of F. hirta Vahl. were analyzed by UPLC-MS. 57 phenolic compounds were identified, among which 49 are novel and reported for the first time. Psoralen was the most common free phenolic, followed by coniferin and bergapten. Coniferin, afzelechin, naringenin, ferulic acid, and umbelliferone were the predominant bound phenolics. Both free and bound phenolic fraction exhibited relatively high capacity to scavenge DPPH free radicals. According to PCA and HCA analysis, free phenolic fraction in five-lobed leaf type were distinct from unlobed and three-lobed. Pearson correlation identified that psoralen and other 18 phenolic compounds were positively correlated with antioxidant activity. These findings can provide theoretical basis for the breeding of F. hirta Vahl. varieties to maximize phenol production for later development and commercial utilization.
根内游离和结合酚类化合物及其抗氧化活性研究。UPLC-MS分析。共鉴定出57种酚类化合物,其中49种为首次报道的新化合物。补骨脂素是最常见的游离酚类物质,其次是松柏素和草莓素。松柏苷、紫荆苷、柚皮苷、阿魏酸和伞形花酮是主要的结合酚类物质。游离和结合酚组分均表现出较高的清除DPPH自由基的能力。主成分分析和HCA分析表明,五裂片叶型的游离酚含量明显高于无裂片叶型和三裂片叶型。Pearson相关分析发现补骨脂素等18种酚类化合物与抗氧化活性呈正相关。这些研究结果可为该品种的选育提供理论依据。品种以最大限度地提高苯酚产量,供后期开发和商业利用。
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引用次数: 0
Characterization of key volatile compounds in meat-based broths using HS-SPME-Arrow-GC/MS and their relationship to sensory perception 用HS-SPME-Arrow-GC/MS表征肉汤中主要挥发性化合物及其与感官知觉的关系
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-02-01 DOI: 10.1016/j.fochx.2026.103601
Jin-Kyung Nam , Mi-Ran Kim , Jeong Eun Hyeon , Hae Won Jang
Meat-based broths are increasingly popular with consumers; however, their volatile profiles remain underexplored. This study uses headspace solid-phase microextraction-Arrow combined with gas chromatography/mass spectrometry to identify and quantify volatile compounds in commercial meat-based broths. The analytical method was optimized using response surface methodology and validated through calibration curves, limits of detection and quantification, and recovery analyses to ensure sensitivity and accuracy. Eighteen volatile compounds were identified, with aldehydes being the most abundant. Several compounds, including aldehydes contributing fatty characteristics, were identified in meat-based broths as well as beef flavoring ingredients (extract and powder), whereas pyrazines and methional were found only in beef flavoring ingredients. In sensory evaluations, samples with strong meaty odors and rich umami tastes received high ratings, whereas weak odors and flavors or low saltiness yielded poor consumer acceptance. By identifying key volatile compounds in meat-based broths, this study provides insight for developing Korean-style home meal replacement products.
肉汤越来越受到消费者的欢迎;然而,它们不稳定的特征仍未得到充分探索。本研究采用顶空固相微萃取-箭头法结合气相色谱/质谱法对商业肉汤中的挥发性化合物进行鉴定和定量。采用响应面法对分析方法进行优化,并通过标定曲线、检出限和定量限以及回收率分析对分析方法进行验证,以保证分析方法的灵敏度和准确性。鉴定出18种挥发性化合物,其中醛类含量最多。在肉汤和牛肉调味料(提取物和粉末)中发现了几种化合物,包括有助于脂肪特征的醛,而吡嗪和甲基硫只在牛肉调味料中发现。在感官评估中,具有强烈肉味和丰富鲜味的样品获得了很高的评价,而较弱的气味和味道或低咸味则产生了较差的消费者接受度。通过鉴定肉汤中的主要挥发性化合物,本研究为开发韩式家庭代餐产品提供了见解。
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引用次数: 0
Mitigating degradation of frying oil and oil absorption of French fries caused by α-tocopherol and γ-oryzanol incorporation into rice bran oil. 减轻米糠油中α-生育酚和γ-谷维醇的掺入对煎炸油的降解和炸薯条的吸油作用。
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-31 eCollection Date: 2026-02-01 DOI: 10.1016/j.fochx.2026.103616
Hai-Long Zhang, Meng-Qing Xu, Kai Zhang, Li-Juan Han, Jing Du, Wei-Nong Zhang

α-Tocopherol and γ-oryzanol affect physicochemical properties of oils, while their role in frying oil uptake remains unclear. Thus, the influence of various concentrations of 0-1000 mg α-tocopherol/kg oil and 0-15,000 mg γ-oryzanol/kg oil on the oil absorption of French fries and possible mechanisms were investigated. The results demonstrated that α-tocopherol and γ-oryzanol reduced surface oil by 29.83% and surface-penetrated oil by 28.19% in the fries, compared with control sample. Furthermore, α-tocopherol and γ-oryzanol inhibited the formation of total polar materials and diglycerides, delayed the increase in oil viscosity, and influenced the porosity of French fries. Additionally, α-tocopherol and γ-oryzanol decreased the rate constants for water loss and oil absorption at the French fry-oil interface. The most significant reduction in total oil content was observed with 1000 mg α-tocopherol/kg oil and 10,000 mg γ-oryzanol/kg oil. This study provides new insights for developing low-oil fried foods.

α-生育酚和γ-谷米醇影响油的理化性质,但它们在煎炸油吸收中的作用尚不清楚。因此,研究了0 ~ 1000 mg α-生育酚/kg油和0 ~ 15000 mg γ-米素/kg油对炸薯条吸油性能的影响及其可能的机理。结果表明,与对照相比,α-生育酚和γ-谷维醇分别减少了薯条表面油的29.83%和表面渗透油的28.19%。α-生育酚和γ-谷维醇抑制了总极性物质和二甘油酯的形成,延缓了油粘度的增加,影响了薯条的孔隙度。此外,α-生育酚和γ-谷维素降低了油炸-油界面的水分损失和吸油速率常数。1000 mg α-生育酚/kg油和10000 mg γ-谷维素/kg油可显著降低总油含量。本研究为开发低油油炸食品提供了新的思路。
{"title":"Mitigating degradation of frying oil and oil absorption of French fries caused by α-tocopherol and γ-oryzanol incorporation into rice bran oil.","authors":"Hai-Long Zhang, Meng-Qing Xu, Kai Zhang, Li-Juan Han, Jing Du, Wei-Nong Zhang","doi":"10.1016/j.fochx.2026.103616","DOIUrl":"https://doi.org/10.1016/j.fochx.2026.103616","url":null,"abstract":"<p><p>α-Tocopherol and γ-oryzanol affect physicochemical properties of oils, while their role in frying oil uptake remains unclear. Thus, the influence of various concentrations of 0-1000 mg α-tocopherol/kg oil and 0-15,000 mg γ-oryzanol/kg oil on the oil absorption of French fries and possible mechanisms were investigated. The results demonstrated that α-tocopherol and γ-oryzanol reduced surface oil by 29.83% and surface-penetrated oil by 28.19% in the fries, compared with control sample. Furthermore, α-tocopherol and γ-oryzanol inhibited the formation of total polar materials and diglycerides, delayed the increase in oil viscosity, and influenced the porosity of French fries. Additionally, α-tocopherol and γ-oryzanol decreased the rate constants for water loss and oil absorption at the French fry-oil interface. The most significant reduction in total oil content was observed with 1000 mg α-tocopherol/kg oil and 10,000 mg γ-oryzanol/kg oil. This study provides new insights for developing low-oil fried foods.</p>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"34 ","pages":"103616"},"PeriodicalIF":8.2,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12891880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146178486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hyperspectral imaging with machine learning for non-destructive prediction of quality attributes in Lanzhou frozen pears. 基于机器学习的高光谱成像兰州冻梨品质属性无损预测。
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-31 eCollection Date: 2026-02-01 DOI: 10.1016/j.fochx.2026.103607
Weihao Qiao, Mei Yang, Jingming Hu, Huili Yan, Yeming Guo, Faxu Guo

Frozen pears undergo complex internal quality changes during frozen storage, while conventional evaluation methods are destructive and time-consuming, limiting rapid or continuous monitoring. This study employed hyperspectral reflectance imaging combined with spectral preprocessing, feature wavelength selection, and machine learning algorithms to model key quality indicators of frozen pears, including soluble solids content (SSC), titratable acidity (TA), and total sugar content (TSC). The results revealed distinct optimal modeling strategies for different quality attributes. Stable predictions of TSC were achieved using full-spectrum data or weak wavelength screening, whereas SSC and TA required targeted wavelength optimization to improve prediction robustness. The optimal models exhibited high coefficients of determination, low prediction errors, and acceptable RPD values across prediction sets. Further validation using the guidance method confirmed stable error distributions without evident overfitting. Overall, this study demonstrates that hyperspectral imaging can provide a reliable alternative for non-destructive quality assessment of Lanzhou frozen pears.

冷冻梨在贮藏过程中会发生复杂的内部质量变化,而传统的评价方法具有破坏性和耗时,限制了快速或连续的监测。本研究采用高光谱反射成像技术,结合光谱预处理、特征波长选择和机器学习算法,对冷冻梨的可溶性固形物含量(SSC)、可滴定酸度(TA)和总糖含量(TSC)等关键质量指标进行建模。结果表明,不同质量属性下的最优建模策略不同。TSC的稳定预测是通过全光谱数据或弱波长筛选实现的,而SSC和TA需要有针对性的波长优化来提高预测的鲁棒性。最优模型具有较高的决定系数、较低的预测误差和可接受的RPD值。使用制导方法进一步验证,确认了稳定的误差分布,没有明显的过拟合。综上所述,高光谱成像技术可以为兰州冻梨的无损品质评价提供可靠的替代方法。
{"title":"Hyperspectral imaging with machine learning for non-destructive prediction of quality attributes in Lanzhou frozen pears.","authors":"Weihao Qiao, Mei Yang, Jingming Hu, Huili Yan, Yeming Guo, Faxu Guo","doi":"10.1016/j.fochx.2026.103607","DOIUrl":"https://doi.org/10.1016/j.fochx.2026.103607","url":null,"abstract":"<p><p>Frozen pears undergo complex internal quality changes during frozen storage, while conventional evaluation methods are destructive and time-consuming, limiting rapid or continuous monitoring. This study employed hyperspectral reflectance imaging combined with spectral preprocessing, feature wavelength selection, and machine learning algorithms to model key quality indicators of frozen pears, including soluble solids content (SSC), titratable acidity (TA), and total sugar content (TSC). The results revealed distinct optimal modeling strategies for different quality attributes. Stable predictions of TSC were achieved using full-spectrum data or weak wavelength screening, whereas SSC and TA required targeted wavelength optimization to improve prediction robustness. The optimal models exhibited high coefficients of determination, low prediction errors, and acceptable RPD values across prediction sets. Further validation using the guidance method confirmed stable error distributions without evident overfitting. Overall, this study demonstrates that hyperspectral imaging can provide a reliable alternative for non-destructive quality assessment of Lanzhou frozen pears.</p>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"34 ","pages":"103607"},"PeriodicalIF":8.2,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12887812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of endogenous walnut pellicle polyphenols on the structure and functional properties of walnut meal isolate proteins. 内源核桃仁膜多酚对核桃仁分离蛋白结构和功能特性的影响。
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-31 eCollection Date: 2026-02-01 DOI: 10.1016/j.fochx.2026.103624
Yunhua Cao, Yanmei Deng, Guohui Yuan, Songliang Zhao, Qian Ma, JiaojiaoYang, Lei Guo, Fangyu Fan

This study aims to investigate how endogenous walnut pellicle polyphenols (WPP) alter the structural and functional properties of walnut protein isolate (WalPI). WPP influence was assessed by adjusting polyphenol content in walnut meal (WM) through treatments that peeled and dephenolized the pellicle. Results showed that WPP decreased the β-sheet content of unpeeled walnut protein isolate (UWalPI), increased random coil structures, and induced structural unfolding. These structural changes raised the water-holding capacity of UWalPI from 1.72 to 2.48 g/g and enhanced foaming capacity and foam stability by 31.12% and 46.44%, respectively. Conversely, surface hydrophobicity declined by 62.9%, oil-holding capacity reduced to 2.44 g/g, and emulsifying capacity, solubility, and thermal stability were compromised. Scanning electron microscopy indicated WPP-induced rearrangement and aggregation, molecular docking simulations predicted interactions between WPP and WalPI. The results clarify the mechanisms of WPP-WalPI interactions and provide strategies for the application of WM and WalPI in food processing.

本研究旨在研究内源性核桃膜多酚(WPP)如何改变核桃分离蛋白(WalPI)的结构和功能特性。通过对核桃粉进行去皮和脱酚处理,调整核桃粉中多酚含量,评价WPP对核桃粉的影响。结果表明,WPP降低了未剥皮核桃蛋白分离物(UWalPI) β-sheet含量,增加了随机卷曲结构,诱导结构展开。这些结构变化使UWalPI的持水量从1.72 g/g提高到2.48 g/g,发泡能力和泡沫稳定性分别提高了31.12%和46.44%。相反,表面疏水性下降62.9%,持油能力下降至2.44 g/g,乳化能力、溶解性和热稳定性受到影响。扫描电镜显示WPP诱导重排和聚集,分子对接模拟预测WPP和WalPI之间的相互作用。研究结果阐明了WPP-WalPI相互作用的机制,并为WM和WalPI在食品加工中的应用提供了策略。
{"title":"Effects of endogenous walnut pellicle polyphenols on the structure and functional properties of walnut meal isolate proteins.","authors":"Yunhua Cao, Yanmei Deng, Guohui Yuan, Songliang Zhao, Qian Ma, JiaojiaoYang, Lei Guo, Fangyu Fan","doi":"10.1016/j.fochx.2026.103624","DOIUrl":"https://doi.org/10.1016/j.fochx.2026.103624","url":null,"abstract":"<p><p>This study aims to investigate how endogenous walnut pellicle polyphenols (WPP) alter the structural and functional properties of walnut protein isolate (WalPI). WPP influence was assessed by adjusting polyphenol content in walnut meal (WM) through treatments that peeled and dephenolized the pellicle. Results showed that WPP decreased the β-sheet content of unpeeled walnut protein isolate (UWalPI), increased random coil structures, and induced structural unfolding. These structural changes raised the water-holding capacity of UWalPI from 1.72 to 2.48 g/g and enhanced foaming capacity and foam stability by 31.12% and 46.44%, respectively. Conversely, surface hydrophobicity declined by 62.9%, oil-holding capacity reduced to 2.44 g/g, and emulsifying capacity, solubility, and thermal stability were compromised. Scanning electron microscopy indicated WPP-induced rearrangement and aggregation, molecular docking simulations predicted interactions between WPP and WalPI. The results clarify the mechanisms of WPP-WalPI interactions and provide strategies for the application of WM and WalPI in food processing.</p>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"34 ","pages":"103624"},"PeriodicalIF":8.2,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12891869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146178432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Classification and metabolomic profiling of walnut pellicle polyphenols using a Pseudotargeted metabolomics approach. 利用伪靶代谢组学方法对核桃皮膜多酚进行分类和代谢组学分析。
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-30 eCollection Date: 2026-02-01 DOI: 10.1016/j.fochx.2026.103610
Chang Liu, Mingxue Geng, Jiaxin Yin, Huibo Zhao, Bing Qi, Huiqing Li, Di Wang, Yanbing Wu, Shengxing Dai, Min Lu, Kuizhang Yao, Junxia Xia, Jiankang Deng

Walnut pellicle is rich in polyphenols that enhance antioxidant capacity and health benefits, including anti-inflammatory and neuroprotective effects. Profiling these compounds has been hindered by their structural diversity, regional variability, and the limitations of traditional metabolomics approaches. This study employed a pseudotargeted metabolomics strategy, integrating ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) for untargeted profiling and multiple reaction monitoring (MRM) on a QTRAP system for semi-quantification. We analyzed 21 walnut pellicle samples from Xinjiang, Yunnan and the Taihang Mountains, identifying 406 polyphenols, including flavonoids, hydrolysable tannins, phenolic acids, coumarins, lignans and minor constituents. Multivariate analyses (PCA, PLS-DA, OPLS-DA) revealed region-specific metabolic fingerprints. KEGG pathway enrichment highlighted significant variations in flavonoid and phenylpropanoid biosynthesis across regions, with origin-specific markers like casuarinin, prunin, and ε-viniferin supporting provenance authentication. This research bridges the methodological gap in walnut polyphenol analysis and informs quality assurance, targeted breeding, and functional product development in sustainable food systems.

核桃皮含有丰富的多酚,可以增强抗氧化能力和健康益处,包括抗炎和神经保护作用。这些化合物的结构多样性、区域可变性和传统代谢组学方法的局限性阻碍了对它们的分析。本研究采用假靶向代谢组学策略,将超高效液相色谱-四极杆飞行时间质谱(UHPLC-QTOF-MS)整合为非靶向分析,并在QTRAP系统上进行多反应监测(MRM)进行半定量。对新疆、云南和太行山的21份核桃皮样品进行了分析,鉴定出406种多酚类物质,包括黄酮类、水解单宁、酚酸、香豆素、木脂素和少量成分。多变量分析(PCA, PLS-DA, OPLS-DA)揭示了区域特异性代谢指纹图谱。KEGG途径富集表明,不同地区的类黄酮和苯丙素生物合成存在显著差异,而木麻黄素、prunin和ε- vinifin等特异性标记支持出处验证。本研究弥补了核桃多酚分析的方法学差距,并为可持续食品系统中的质量保证、有针对性的育种和功能性产品开发提供了信息。
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引用次数: 0
Effect of different cooking methods on the sensory, nutritional, and flavor profiles of three Cucurbita moschata and Cucurbita maxima cultivars. 不同蒸煮方法对三种甜瓜和大瓜感官、营养和风味的影响。
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-30 eCollection Date: 2026-02-01 DOI: 10.1016/j.fochx.2026.103611
Shudan Xue, Yang Yu, Yingchao Xu, Wenlong Luo, Yingyin Lin, Muhammad Sajjad, Qingmin Jin, Dasen Xie, Yujuan Zhong

Pumpkin (Cucurbita moschata and Cucurbita maxima) is a nutrient-rich vegetable, yet cooking-induced changes in comprehensive quality attributes across cultivars remain poorly understood. Here, the effects of steaming, boiling, and baking on taste components, texture, nutritional quality, and volatile profiles were systematically evaluated in three pumpkin cultivars. Sugar and starch contents were largely unaffected by cooking, whereas textural properties were significantly altered, with baking causing the least structural disruption. Steaming and boiling increased organic acid and vitamin C contents as well as antioxidant activity. Boiling enhanced carotenoid extractability, with Tian Mi highest at 3.13 mg/g DW. Cultivar-dependent differences were observed: Dan Hong showed higher hardness, chewiness, and gumminess, while Zhen Mi exhibited greater total volatile content. Volatile analysis revealed that cooking, particularly boiling, reduced green and earthy odorants while increasing key aroma-active compounds, including furfural and 4-vinylguaiacol. Overall, boiling provided the most favorable balance between nutritional enhancement and aroma improvement.

南瓜(Cucurbita moschata和Cucurbita maxima)是一种营养丰富的蔬菜,但烹饪引起的品种间综合品质属性的变化仍然知之甚少。本研究系统地评价了蒸、煮和烤对三个南瓜品种的口感成分、质地、营养品质和挥发性特征的影响。糖和淀粉含量在很大程度上不受烹饪的影响,而质地特性却发生了显著变化,烘焙对结构的破坏最小。蒸和煮可以增加有机酸和维生素C的含量以及抗氧化活性。蒸煮提高了类胡萝卜素的提取率,其中田密的提取率最高,为3.13 mg/g DW。不同品种间存在差异:丹红具有较高的硬度、嚼劲和黏性,甄密具有较高的总挥发分含量。挥发性分析表明,烹饪,特别是煮沸,减少了绿色和泥土的气味,同时增加了关键的芳香活性化合物,包括糠醛和4-乙烯基愈创木酚。总的来说,煮在营养增强和香气改善之间提供了最有利的平衡。
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引用次数: 0
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Food Chemistry: X
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