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Impact of harvest periods of Sichuan pepper on Sichuan pepper-flavoured chicken using electronic nose, electronic tongue, and chromatography 利用电子鼻、电子舌和色谱法研究花椒采收期对花椒味鸡的影响
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103413
Lian He , Zongyuan Lu , Xuemei Cai , Kaixian Zhu , Chengjian Xu , Dan Lin , Zhiyong Hou , Sze Ying Leong , Nallammai Singaram , Cui Yang , Sook Wah Chan , Hua Peng , Minglu Lan
This study compared Sichuan pepper harvested at five stages (July–September) for preparing Sichuan pepper-flavoured chicken. Electronic nose and tongue analyses separated the control from peppered samples and further distinguished early (July–august) from late (September) harvests; September pepper produced stronger odour, higher umami and sourness, and lower bitterness. GC–IMS fingerprints revealed harvest-dependent shifts in volatile profiles, and OPLS-DA (VIP > 1) highlighted limonene-D/M (highest VIP), terpinolene, myrcene, sec-butanol, isobutyl acetate-D, and terpinyl acetate-D/M as key markers. HS–GC–MS detected 68 volatiles; OPLS-DA markers (VIP > 1) included 3-penten-2-ol (highest VIP), β-pinene, limonene, phellandrene, linalyl acetate, and eucalyptol, while α-pinene varied significantly with harvest period. Overall, later harvest increased terpene/ester abundance and most effectively enhanced the characteristic flavour of Sichuan pepper-flavoured chicken.
本研究比较了5个阶段(7 - 9月)收获的花椒制作花椒香鸡的效果。电子鼻和电子舌分析将对照样品与胡椒样品分开,并进一步区分早期(7 - 8月)和晚期(9月)的收获;9月辣椒的气味更浓,鲜味和酸味更高,苦味更低。GC-IMS指纹图谱揭示了挥发性特征的收获依赖性变化,OPLS-DA (VIP > 1)突出了柠檬烯-d /M(最高VIP)、松油烯、桃金桃烯、中丁醇、醋酸异丁酯-d和醋酸松油酯-d /M作为关键标记。HS-GC-MS检出68种挥发物;OPLS-DA标记(VIP > 1)包括3-戊烯-2-醇(VIP最高)、β-蒎烯、柠檬烯、茶香烯、醋酸芳樟烯和桉树油,α-蒎烯随采收期变化显著。总体而言,后期收获增加了萜烯/酯丰度,最有效地增强了花椒味鸡的特色风味。
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引用次数: 0
Effects of Coriolus versicolor-fermented sweet potato pulp water on yogurt: bioactive components, functional properties, and in vitro gut microbiota modulation 红薯浆水对酸奶的影响:生物活性成分、功能特性和体外肠道菌群调节
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103425
Qin Cen , Jiamin Li , Zhengbin Yang , Zefen Zhu , Juan Zhou , Xinyao Huang , Huaimao Tie , Xuefeng Zeng , Likang Qin
In the sweet potato starch processing industry, large volumes of organic wastewater are generated, yet their utilization remains limited. This study introduces a novel strategy for yogurt enhancement by incorporating Coriolus versicolor-fermented sweet potato pulp water (CV-SPPW). The effects of different CV-SPPW concentrations on yogurt's bioactive compounds, functional properties, in vitro gut microbiota modulation were evaluated. The results revealed that increasing CV-SPPW concentrations enriched bioactive components, including polysaccharides and triterpenoids. Notably, at a concentration of 15 %, organic acids such as lactic acid (0.6991 mg/mL) and succinic acid (0.0167 mg/mL) were maximized. Yogurt supplemented with 15 % CV-SPPW exhibited enhanced antioxidant and hypoglycemic activities. Furthermore, CV-SPPW supplementation modulated the gut microbiota by increasing the relative abundance of beneficial phyla, such as Firmicutes and Bacteroidetes, while reducing potentially harmful taxa, thereby improving microbial diversity and community structure. These findings suggest that CV-SPPW valorization provides a sustainable approach to developing functional yogurt with enhanced health benefits.
甘薯淀粉加工行业产生大量有机废水,但其利用仍然有限。本研究介绍了一种添加花斑香菇发酵甘薯浆水(CV-SPPW)增强酸奶的新策略。研究了不同浓度的CV-SPPW对酸奶生物活性成分、功能特性和体外肠道菌群调节的影响。结果表明,随着CV-SPPW浓度的增加,其多糖和三萜等生物活性成分含量增加。值得注意的是,当浓度为15%时,乳酸(0.6991 mg/mL)和琥珀酸(0.0167 mg/mL)等有机酸达到最大值。添加15% CV-SPPW的酸奶具有增强的抗氧化和降糖活性。此外,添加CV-SPPW可以通过增加有益门(如厚壁菌门和拟杆菌门)的相对丰度来调节肠道微生物群,同时减少潜在的有害分类群,从而改善微生物多样性和群落结构。这些发现表明,CV-SPPW增值为开发具有增强健康益处的功能性酸奶提供了一种可持续的方法。
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引用次数: 0
Changes in nutritional and volatile composition of peach puree as affected by fermentation with different Lactobacillus strains and subsequent cold storage 不同乳酸菌发酵和冷藏对桃果泥营养成分和挥发性成分的影响
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103441
Qiaoyu Han , Jiechao Liu , Muhammad Nawaz , Hui Liu , Qiang Zhang , Zhenzhen Lv , Dalei Chen , Wenbo Yang , Zhonggao Jiao
The peach puree was fermented by 4 selected Lactobacillus strains, the changes in sugars, acids, phenolics, carotenoids, and volatile composition after fermentation and subsequent cold storage (4 °C) were investigated. Results found that fermentation with different Lactobacillus strains resulted in a sugar consumption (2.64–7.62 g/L) and lactic acid production (1.72–9.69 g/L). Fermentation increased the levels of total phenolics (5.31–28.07 %), total carotenoids (12.34–43.16 %), total volatiles (1.59–4.78 times), and antioxidant capacity (6.48–20.32 %) relative to control sample, with L. plantarum 21802 exhibiting the greatest enhancement. During storage, the nutritional and volatile composition underwent a slightly continuous change, depending on the Lactobacillus strains and storage time. The antioxidant activity of L. plantarum 21802 group was 18.35 % (14 d) and 20.61 % (28 d) higher than control. These findings confirm that the evolutions of phytochemical composition in peach puree were various from strains and L. plantarum 21802 was a promising strain for producing functional peach puree.
选用4株乳酸菌发酵桃果泥,研究了发酵后和4℃冷藏后桃果泥中糖、酸、酚类、类胡萝卜素和挥发性成分的变化。结果表明,不同菌株发酵的糖消耗量为2.64 ~ 7.62 g/L,乳酸产量为1.72 ~ 9.69 g/L。发酵后总酚类物质含量(5.31 ~ 28.07%)、总类胡萝卜素含量(12.34 ~ 43.16%)、总挥发物含量(1.59 ~ 4.78倍)和抗氧化能力(6.48 ~ 20.32%)均较对照提高,其中以L. plantarum 21802提高幅度最大。在储存过程中,营养成分和挥发性成分发生了轻微的连续变化,这取决于乳酸菌菌株和储存时间。21802组的抗氧化活性分别比对照组高18.35% (14 d)和20.61% (28 d)。这些结果证实了不同菌株桃果泥中植物化学成分的进化是不同的,植物乳杆菌21802是一种有潜力生产功能性桃果泥的菌株。
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引用次数: 0
Deciphering the impact of air frying vs. oven roasting on crayfish (Procambarus clarkii) quality: A multiscale journey from water migration and protein denaturation to texture, flavor, and digestibility 解读空气煎炸与烤箱烤对克氏原螯虾(Procambarus clarkii)品质的影响:从水分迁移和蛋白质变性到质地、风味和消化性的多尺度旅程
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2026.103495
Wensi Xu , Shengcai Xu , Aihua Deng , Xiaoyang Liu , Liang Song , Dayong Zhou , Qifu Yang
Crayfish are valued for their culinary appeal and nutritional profile. This study compares air frying (AF) and roasting (RO) impacts on crayfish muscle. We investigated water migration, protein structural changes, microstructure, sensory attributes, digestibility, and volatile compounds. Dry-heat processing induced significant protein oxidation and aggregation, as evidenced by a 3.6-fold increase in carbonyl content, a 48 % rise in the myofibrillar fragmentation index, and a reduction in free sulfhydryl groups. AF promoted protein oxidation due to rapid heating, whereas RO enhanced proteolysis. AF samples retained bound water within compact matrices, restricting water mobility, whereas RO formed porous networks that increased free water content, yielding smaller digesta particles (367.9 nm). AF enriched volatile compounds through dehydration-driven concentration, with tetrahydro-2-furanmethanol as a discriminant marker. AF at 170 °C/10 min achieved optimal sensory acceptance. Our findings elucidate multiscale mechanisms linking water-protein dynamics to texture, flavor, and digestibility, providing a scientific basis for optimizing crustacean processing.
小龙虾因其烹饪吸引力和营养价值而受到重视。本研究比较了空气煎炸(AF)和烧烤(RO)对小龙虾肌肉的影响。我们研究了水分迁移、蛋白质结构变化、微观结构、感官属性、消化率和挥发性化合物。干热处理诱导了显著的蛋白质氧化和聚集,羰基含量增加3.6倍,肌纤维断裂指数增加48%,游离巯基减少。AF由于快速加热而促进蛋白质氧化,而RO则促进蛋白质水解。AF样品在紧凑的基质中保留了束缚水,限制了水的流动性,而RO形成的多孔网络增加了自由水的含量,产生更小的消化颗粒(367.9 nm)。AF通过脱水驱动浓度富集挥发性化合物,以四氢-2-呋喃甲醇作为判别标记。170°C/10分钟的AF达到最佳感官接受度。我们的研究结果阐明了水蛋白动力学与质地、风味和消化率之间的多尺度机制,为优化甲壳类动物的加工提供了科学依据。
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引用次数: 0
Aroma profiling using GC E-nose to trace origin of polygonati rhizoma species and quantify the effect of using ‘nine steaming and nine sun drying” processing 利用气相色谱电子鼻对黄精进行香气分析,追溯黄精品种的产地,量化“九蒸九晒”加工的效果
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103463
Maidinai Sabier , David D. Kitts , Aobo Zhu , Suying Huang , Yue Li , Pan Wang , Jinping Si , Xinfeng Zhang
Aroma evolution during the traditional Nine Steaming and Nine Drying (NSND) process is essential to establish quality control and authentication of Polygonatum rhizome (PR) species, yet the underlying chemical mechanisms underlying changes remain poorly characterized. Using a flash GC–E-nose, we profiled volatile transitions of four PR species across ten NSND stages and tentatively identified 71 compounds. Distinct aroma trajectories were observed: early-stage loss of green aldehydes and alcohols reflected suppression of lipid-oxidation pathways, whereas later-stage increases in roasted, aromatic, and sulfur-derived notes were consistent with progressive Maillard reactions, sugar fragmentation, and Strecker degradation. Species-specific differences, including ester, ketone, and phenolic formation, further suggested variations in precursor pools and thermal reactivity. Multivariate analyses (ANOVA, PCA, HCA) clearly discriminated species and steaming stages, revealing consistent chemical transformation patterns. These mechanistic insights provide foundational volatile profiling knowledge that will support future development of aroma-based tools for PR quality evaluation and authentication.
传统九蒸九干(NSND)工艺的香气演变是建立黄精根(PR)品种质量控制和认证的必要条件,但其变化的化学机制尚不清楚。利用瞬时气相色谱-电子鼻,我们分析了4种PR在10个NSND阶段的挥发性转变,并初步鉴定了71种化合物。不同的香气轨迹被观察到:早期绿醛和醇的损失反映了脂质氧化途径的抑制,而后期烘焙、芳香和硫衍生气味的增加与渐进的美拉德反应、糖碎裂和斯特雷克降解一致。物种特异性差异,包括酯、酮和酚的形成,进一步表明前体池和热反应性的差异。多变量分析(ANOVA, PCA, HCA)清晰地区分了物种和蒸熟阶段,揭示了一致的化学转化模式。这些机制见解提供了基本的挥发性分析知识,将支持未来开发用于PR质量评估和认证的基于芳香的工具。
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引用次数: 0
Study on the effects of different fermentation methods on fermented mustard microbial community composition and metabolite profile 不同发酵方式对发酵芥菜微生物群落组成及代谢产物谱的影响研究
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103468
Yaoying Zeng , Yuheng Tan , Le Xie , Yubo Xiong , Zhuojun Yu , Ming Jia , Wei He , Wenhua Zhou
Fermented mustard is a traditional Chinese vegetable product with complex flavor profiles shaped primarily by microbial activity during fermentation. Although several studies focus on single fermentation systems, few have integrated microbial diversity and metabolomics. This study investigated the effects of three fermentation techniques, i.e., jar fermentation (JFM), pool fermentation (PFM), and bury fermentation (BFM), on the quality attributes, microbial community composition, and metabolite profile of fermented mustard. JFM was predominantly characterized by Lactobacillus and Wickerhamiella, creating a stable microaerobic environment that promoted lactic acid accumulation and ester synthesis. BFM showed higher abundance of potential pathogens such as Staphylococcus and Pseudomonas, suppressing lactic acid bacteria (LAB) activity. Metabolomics revealed 8447 metabolites, with lipids, organic heterocyclic compounds, and phenylpropanoids as key flavor contributors. These findings establish that JFM's microaerobic environment selectively enriches beneficial Lactobacillus-Wickerhamiella consortia while suppressing spoilage taxa, driving superior quality and safety through distinct metabolic pathways. This comparative framework provides targeted strategies for optimizing traditional mustard fermentation.
发酵芥菜是一种传统的中国蔬菜产品,具有复杂的风味特征,主要由发酵过程中的微生物活动形成。虽然一些研究集中在单一的发酵系统,很少有整合微生物多样性和代谢组学。研究了罐式发酵(JFM)、池式发酵(PFM)和埋式发酵(BFM) 3种发酵工艺对发酵芥菜品质属性、微生物群落组成和代谢产物谱的影响。JFM以乳酸杆菌和Wickerhamiella菌为主,形成了稳定的微氧环境,促进了乳酸积累和酯合成。BFM中潜在病原菌如葡萄球菌和假单胞菌丰度较高,可抑制乳酸菌(LAB)活性。代谢组学发现了8447种代谢物,其中脂质、有机杂环化合物和苯丙素是主要的风味贡献者。这些发现表明,JFM的微氧环境选择性地丰富了有益的乳酸杆菌- wickerhamiella联合体,同时抑制了腐败分类群,通过不同的代谢途径实现了卓越的质量和安全性。这个比较框架为优化传统芥菜发酵提供了有针对性的策略。
{"title":"Study on the effects of different fermentation methods on fermented mustard microbial community composition and metabolite profile","authors":"Yaoying Zeng ,&nbsp;Yuheng Tan ,&nbsp;Le Xie ,&nbsp;Yubo Xiong ,&nbsp;Zhuojun Yu ,&nbsp;Ming Jia ,&nbsp;Wei He ,&nbsp;Wenhua Zhou","doi":"10.1016/j.fochx.2025.103468","DOIUrl":"10.1016/j.fochx.2025.103468","url":null,"abstract":"<div><div>Fermented mustard is a traditional Chinese vegetable product with complex flavor profiles shaped primarily by microbial activity during fermentation. Although several studies focus on single fermentation systems, few have integrated microbial diversity and metabolomics. This study investigated the effects of three fermentation techniques, i.e., jar fermentation (JFM), pool fermentation (PFM), and bury fermentation (BFM), on the quality attributes, microbial community composition, and metabolite profile of fermented mustard. JFM was predominantly characterized by <em>Lactobacillus</em> and <em>Wickerhamiella,</em> creating a stable microaerobic environment that promoted lactic acid accumulation and ester synthesis. BFM showed higher abundance of potential pathogens such as <em>Staphylococcus</em> and <em>Pseudomonas</em>, suppressing lactic acid bacteria (LAB) activity. Metabolomics revealed 8447 metabolites, with lipids, organic heterocyclic compounds, and phenylpropanoids as key flavor contributors. These findings establish that JFM's microaerobic environment selectively enriches beneficial <em>Lactobacillus-Wickerhamiella</em> consortia while suppressing spoilage taxa, driving superior quality and safety through distinct metabolic pathways. This comparative framework provides targeted strategies for optimizing traditional mustard fermentation.</div></div>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"33 ","pages":"Article 103468"},"PeriodicalIF":8.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sustained-release application of porous starch/gum arabic microencapsulated sea buckthorn essential oil in yak meat preservation 多孔淀粉/阿拉伯胶微胶囊沙棘精油在牦牛肉保鲜中的缓释应用
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103325
Jiajia Mo , Rongsheng Du , Kun Wang , Xinying Wang , Kunweng Puguan , Caihui Wang , Yuan Liu , Linlin Wang , Lina Wang
This study prepared microcapsules using sea buckthorn essential oil (SBEO) as the core material and gum arabic with porous starch as wall material via complex coacervation (with successful encapsulation confirmed by FTIR spectroscopy), and further explored its sustained-release application in yak meat preservation (from 0 to 8 days). Their effect on yak meat preservation during 0–8 days refrigerated storage was evaluated. Physicochemical parameters in groups (Control, SB oil, PS: starch+ SB, WS: microcapsules, WP: starch + microcapsules) were measured. All SB-containing groups maintained color/ moisture, delayed pH rise, inhibited microbes, and mitigated myofibrillar protein (MP) oxidative damage. WP exhibited superior preservation: significantly better at stabilizing color/ moisture, inhibiting MP oxidation while preserving its secondary/ tertiary structures. Via sustained release, WP prolonged antioxidant/ antibacterial activity, thus significantly improve the quality stability of yak meat during storage. The porous starch combined with microencapsulated oil (WP) proved optimal for enhancing yak meat storage quality.
本研究以沙棘精油(SBEO)为核心材料,以多孔淀粉为壁材的阿拉伯胶为复合凝聚法制备微胶囊(FTIR光谱证实包封成功),并进一步探讨其在牦牛肉保鲜(0 ~ 8天)中的缓释应用。评价了它们对牦牛肉冷藏0 ~ 8 d保鲜的影响。测定各组(对照、SB油、PS:淀粉+ SB、WS:微胶囊、WP:淀粉+微胶囊)理化参数。所有含有sb的组都保持了颜色/水分,延缓了pH值的上升,抑制了微生物,减轻了肌纤维蛋白(MP)的氧化损伤。WP具有优异的保鲜性:在稳定颜色/水分,抑制MP氧化的同时保留其二级/三级结构方面明显更好。通过缓释作用,可延长牦牛肉的抗氧化/抗菌活性,从而显著提高牦牛肉在贮藏过程中的品质稳定性。多孔淀粉与微囊化油(WP)复合是提高牦牛肉贮藏品质的最佳选择。
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引用次数: 0
Novel Lys-blocking-Thr oligopeptide coating for extended banana preservation and quality retention 新型赖氨酸阻断-苏寡肽包衣,延长香蕉的保存和品质保持
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103419
Bo Xia , Honghao Chen , Yan Liu , Li Fan , Zhewei Shi , Tianfeng Chen , Jianfeng Wang , Guilin Cheng
Bananas, the world's fourth-largest food crop, generate $44.1 billion annually and feed over 400 million people. However, postharvest preservation remains challenging due to rapid climacteric ripening. Current preservation strategies using polysaccharides and synthetic polymers only provide film-forming properties, requiring additional bioactive components (phytochemicals) or other agents (metal nanoparticles), to achieve preservation effects. In contrast, peptides possess intrinsic antibacterial and antioxidant properties enabling independent preservation effects yet remain unexplored for commercial banana preservation despite proven potential in other food applications. We report a novel Lys-blocking-Thr oligopeptide coating synthesized via ring-opening polymerization that significantly extends banana shelf life. The oligopeptide coating extended shelf life from 4 (controls) to 8 days under ambient conditions while reducing weight loss by 50 % and preserving nutritional components including titratable acidity, reducing sugars, and total phenolic compounds. This peptide-based approach represents a breakthrough in sustainable postharvest technology, offering enhanced biocompatibility and biodegradability for global banana preservation systems.
香蕉是世界第四大粮食作物,每年产生441亿美元的收入,养活4亿多人。然而,由于快速的更年期成熟,采后保存仍然具有挑战性。目前使用多糖和合成聚合物的保存策略只提供成膜特性,需要额外的生物活性成分(植物化学物质)或其他试剂(金属纳米颗粒)来达到保存效果。相比之下,多肽具有内在的抗菌和抗氧化特性,能够独立保存香蕉,尽管在其他食品应用中具有潜力,但仍未探索商业化香蕉保存。我们报道了一种通过开环聚合合成的新型赖氨酸阻断苏寡肽涂层,该涂层显着延长了香蕉的保质期。寡肽涂层在环境条件下将保质期从4天(对照组)延长至8天,同时减少50%的体重减轻,并保留营养成分,包括可滴定的酸度,还原糖和总酚类化合物。这种基于肽的方法代表了可持续采后技术的突破,为全球香蕉保存系统提供了增强的生物相容性和生物降解性。
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引用次数: 0
The physiochemical characteristics and metabolic components of Paeonia ostii petal tea produced through Eurotium cristatum-mediated liquid fermentation 冠状Eurotium介导液体发酵法生产芍药花瓣茶的理化特性及代谢成分研究
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103436
Xiuxia Ren , Hairong Xiong , Xueping Liao , Ayman Mohamed Ahmed Abduallah , Jingqi Su , Xiaoxiao Wang , Caihuan Tian , Jingqi Xue , Zihan Song , Xiuxin Zhang
Fermentation can change tea flavour. This study aimed to improve the quality of tree peony flower tea through fermentation and investigate the underlying metabolomic changes. Eurotium cristatum were identified from three commercial Fu brick teas and ten strains were subsequently used for fermentation to produce instant peony dark tea (IPDT). It found that fermentation significantly improved the quality of IPDT by reducing astringency, improving the taste, and enhancing the antioxidant and antibacterial activity. Moreover, total sugar content and free amino acids content were reduced in IPDT, while total polyphenols and total flavonoids content were increased. A total of 84 metabolites were identified, which were mainly enriched in carbon metabolism and amino acid metabolism. The improvement of tea flavour after fermentation was clearly explained through “metabolic pathways - metabolic substance concentration - sensory evaluation” model. This study provides technical guidance for IPDT production and enhances comprehensive utilization of peony petals.
发酵可以改变茶的味道。本研究旨在通过发酵提高牡丹花茶的品质,并探讨其代谢组学变化。从3种市售茯砖茶中鉴定出冠状Eurotium cristatum,并利用10株菌种发酵生产速溶牡丹黑茶(IPDT)。研究发现,发酵通过减少涩味、改善口感、增强抗氧化和抗菌活性,显著改善了IPDT的品质。此外,IPDT降低了总糖含量和游离氨基酸含量,提高了总多酚和总黄酮含量。共鉴定出84种代谢物,主要富集于碳代谢和氨基酸代谢。通过“代谢途径-代谢物质浓度-感官评价”模型对发酵后茶叶风味的改善进行了清晰的解释。本研究为牡丹花瓣IPDT的生产提供了技术指导,促进了牡丹花瓣的综合利用。
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引用次数: 0
Impact of ultrasound–enzyme pretreatment sequence on recovery and functionality of proteins from an oat fiber-rich side stream 超声-酶预处理序列对燕麦富含纤维侧流蛋白质恢复和功能的影响
IF 8.2 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2026-01-01 DOI: 10.1016/j.fochx.2025.103452
José Villacís-Chiriboga, Helga Guðný Elíasdóttir, Isabel Badager, Mehdi Abdollahi
The residual fiber-rich fraction after oat protein extraction is a promising resource for sustainable protein recovery, though its rigid protein–fiber matrix hinders extraction. This study assessed effects of mechanical (wet milling, ultrasound) and biochemical (Viscozyme®) pretreatments, individually and in sequence, on its protein recovery using alkaline extraction and isoelectric precipitation. Wet milling slightly improved yields via particle size reduction, while ultrasound alone enhanced protein purity. The combination of ultrasound and enzymatic treatment doubled protein recovery and mass yield, also boosting gelation and viscoelastic strength. Pretreatment order was crucial: ultrasound followed by enzyme treatment yielded higher surface charge and gel strength, whereas enzyme-first produced finer protein particles with moderate gel strength. Ultrasound-first treatments showed lowest phytate retention, enzyme-first the highest. These results reveal the synergistic, sequence-dependent effects of ultrasound and enzymatic pretreatments in releasing protein from fiber-rich oat side stream, supporting their upcycling into functional, plant-based food ingredients.
燕麦蛋白提取后的富纤维残余部分是一种有前途的可持续蛋白质回收资源,但其刚性的蛋白纤维基质阻碍了提取。本研究评估了机械(湿磨、超声波)和生化(粘酶®)预处理分别和依次对其进行碱性提取和等电沉淀的蛋白质回收的影响。湿磨通过减小颗粒大小略微提高了产量,而超声波单独提高了蛋白质纯度。超声波和酶处理的结合使蛋白质回收率和质量收率加倍,也提高了凝胶和粘弹性强度。预处理顺序是至关重要的:超声之后的酶处理产生更高的表面电荷和凝胶强度,而酶先产生更细的蛋白质颗粒,凝胶强度中等。超声优先处理的植酸保留率最低,酶优先处理的植酸保留率最高。这些结果揭示了超声和酶预处理在富含纤维的燕麦侧流中释放蛋白质的协同、序列依赖效应,支持其升级循环为功能性的植物性食品成分。
{"title":"Impact of ultrasound–enzyme pretreatment sequence on recovery and functionality of proteins from an oat fiber-rich side stream","authors":"José Villacís-Chiriboga,&nbsp;Helga Guðný Elíasdóttir,&nbsp;Isabel Badager,&nbsp;Mehdi Abdollahi","doi":"10.1016/j.fochx.2025.103452","DOIUrl":"10.1016/j.fochx.2025.103452","url":null,"abstract":"<div><div>The residual fiber-rich fraction after oat protein extraction is a promising resource for sustainable protein recovery, though its rigid protein–fiber matrix hinders extraction. This study assessed effects of mechanical (wet milling, ultrasound) and biochemical (Viscozyme®) pretreatments, individually and in sequence, on its protein recovery using alkaline extraction and isoelectric precipitation. Wet milling slightly improved yields <em>via</em> particle size reduction, while ultrasound alone enhanced protein purity. The combination of ultrasound and enzymatic treatment doubled protein recovery and mass yield, also boosting gelation and viscoelastic strength. Pretreatment order was crucial: ultrasound followed by enzyme treatment yielded higher surface charge and gel strength, whereas enzyme-first produced finer protein particles with moderate gel strength. Ultrasound-first treatments showed lowest phytate retention, enzyme-first the highest. These results reveal the synergistic, sequence-dependent effects of ultrasound and enzymatic pretreatments in releasing protein from fiber-rich oat side stream, supporting their upcycling into functional, plant-based food ingredients.</div></div>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"33 ","pages":"Article 103452"},"PeriodicalIF":8.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Food Chemistry: X
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