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Comparative study of polysaccharide metabolites in purple, orange, and white Ipomoea batatas tubers 紫色、橙色和白色紫花苕块茎中多糖代谢物的比较研究
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-25 DOI: 10.1016/j.fochx.2024.101855
Xiuzhi Wang, Xiaolin Wan, Jiaqi Wu, Lingjun Cui, Qiang Xiao
We employed LC-MS/MS to investigate the metabolic profiles of polysaccharide compounds in white, orange, and purple sweet potato flesh. Comparisons between Orange vs White, Purple vs Orange, and Purple vs White identified 69 polysaccharide metabolites, including 23, 36, and 44 differential metabolites, respectively, with distinct differentiation. Among the three sample groups, 14 polysaccharide compounds and 2 anthocyanins exhibited significant differences. Our further analysis indicated that anthocyanins occupy a central position in the related network diagram and are interconnected with polysaccharides. In metabolic pathways, sucrose and the anthocyanin precursor UDP-glucose were upregulated in purple sweet potatoes, along with elevated levels of pelargonidin 3-O-β-D-sambubioside and delphinidin 3,5-diglucoside. Conversely, sucrose was downregulated in purple sweet potatoes while increasing in white and orange varieties. Therefore, we hypothesize that the competition between sugars and anthocyanins for shared biosynthesis precursors is attributed to differential polysaccharide metabolites among sweet potato tubers of three colors.
我们采用 LC-MS/MS 方法研究了白色、橙色和紫色甘薯果肉中多糖化合物的代谢概况。橙色与白色、紫色与橙色、紫色与白色之间的比较发现了 69 种多糖代谢物,其中差异代谢物分别为 23、36 和 44 种,且差异明显。在三组样品中,有 14 种多糖化合物和 2 种花青素表现出显著差异。我们的进一步分析表明,花青素在相关网络图中占据中心位置,并与多糖相互关联。在代谢途径中,蔗糖和花青素前体 UDP-葡萄糖在紫甘薯中上调,同时,3-O-β-D-桑布糖苷和 3,5-二葡萄糖苷的水平也升高。相反,紫色甘薯中的蔗糖含量下降,而白色和橙色品种中的蔗糖含量上升。因此,我们推测糖类和花青素之间对共享生物合成前体的竞争是导致三种颜色甘薯块茎中多糖代谢物差异的原因。
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引用次数: 0
Ponceau 4R elimination from fruit juice: An integrated optimization strategy utilizing artificial neural networks, least squares, and chitosan-nickel ferrite Nano Sorbent 消除果汁中的庞西欧 4R:利用人工神经网络、最小二乘法和壳聚糖-镍铁氧体纳米吸附剂的综合优化策略
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-25 DOI: 10.1016/j.fochx.2024.101856
Samira Shokri , Nabi Shariatifar , Ebrahim Molaee-Aghaee , Gholamreza Jahed Khaniki , Parisa Sadighara , Somaye Vali Zade , Shahram Shoeibi
The goal of present work is to examine the efficiency of aminated-chitosan/NiFe2O4 nanoparticles (AmCs/NiFe2O4 NPs) produced for removing Ponceau 4R (P4R) from fruit juice through an adsorption process. The resulting nanoparticles were characterized using various techniques. The modeling of results was done using least squares (LS) and Radial basis function-artificial neural network (RBF-ANN). The optimum removal of P4R (91.43 %) was obtained at the following optimum conditions: pH 4.47, adsorbent dosage 0.047 g/L, contact time approximately 57.78 min, and initial concentration P4R 26.89 mg/L. The highest adsorption capacity (qm) was found to be 208.33 mg g−1. The P4R adsorption mostly followed the Freundlich and pseudo-second-order isotherm kinetic models. Both LS-based models and RBF-ANN provided good predictions for independent variables. The dye elimination efficacy for juice samples were approximately 90.34 %. Therefore, based on the obtained results, it can be claimed that the prepared AmCs/NiFe2O4 NPs can be used to remove P4R.
本研究的目的是考察通过吸附工艺生产的氨基化壳聚糖/NiFe2O4 纳米粒子(AmCs/NiFe2O4 NPs)去除果汁中的 Ponceau 4R (P4R)的效率。利用各种技术对制备的纳米粒子进行了表征。使用最小二乘法(LS)和径向基函数-人工神经网络(RBF-ANN)对结果进行建模。在 pH 值为 4.47、吸附剂用量为 0.047 克/升、接触时间约为 57.78 分钟、初始 P4R 浓度为 26.89 毫克/升的最佳条件下,P4R 的去除率达到了 91.43%。最高吸附容量(qm)为 208.33 mg g-1。P4R 吸附大多遵循 Freundlich 和假二阶等温线动力学模型。基于 LS 的模型和 RBF-ANN 模型都能很好地预测自变量。果汁样品的染料去除率约为 90.34%。因此,根据所获得的结果,可以认为所制备的 AmCs/NiFe2O4 NPs 可用于去除 P4R。
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引用次数: 0
Potassium chloride-assisted heat treatment enhances the de-glycosylation efficiency and xanthine oxidase inhibitory activity of Sophora japonica L. flavonoids 氯化钾辅助热处理可提高槐黄酮的去糖基化效率和黄嘌呤氧化酶抑制活性
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-24 DOI: 10.1016/j.fochx.2024.101854
Jun Li , Peng Wu , Jing Wang , Xiangren Meng , Yang Ni , Liuping Fan
Salt-assisted heat treatment is considered an effective way to enhance the bioactivities of flavonoids in Flos Sophorae Immaturus tea (FSIt). Herein, sodium chloride (NaCl)- and potassium chloride (KCl)-assisted heat treatment was employed to process FSIt, the components, xanthine oxidase (XO) inhibitory activity, and degradation or conversion kinetics of FSIt flavonoids were recorded. Results showed that KCl-assisted heat treatment significantly increased the XO inhibition rate of FSIt from 28.05 % to 69.50 %. The de-glycosylation of flavonoids was the crucial reason for enhancing XO inhibitory activity. Notably, KCl exhibited a better catalytic effect on the de-glycosylation reaction than NaCl. Meanwhile, conversion kinetics showed that the generation rate of quercetin, kaempferol, and isorhamnetin reached the maximum at 180, 160, 160 °C, respectively. Furthermore, the established artificial neural network model could accurately predict the changes of FSIt flavonoids during salt-assisted heat treatment. Thus, KCl can be used as a valuable food processing adjuvant to enhance the bioactivities of food materials.
盐辅助热处理被认为是提高Flos Sophorae Immaturus茶(FSIt)中黄酮类化合物生物活性的有效方法。本文采用氯化钠(NaCl)和氯化钾(KCl)辅助热处理的方法处理花茶,记录了花茶中黄酮类化合物的成分、黄嘌呤氧化酶(XO)抑制活性、降解或转化动力学。结果表明,KCl辅助热处理可将FSIt的XO抑制率从28.05%显著提高到69.50%。黄酮类化合物的去糖基化是提高 XO 抑制活性的关键原因。值得注意的是,氯化钾对去糖基化反应的催化作用优于氯化钠。同时,转化动力学表明,槲皮素、山柰醇和异鼠李素的生成率分别在 180、160 和 160 ℃ 时达到最大值。此外,所建立的人工神经网络模型可以准确预测盐辅助热处理过程中 FSIt 类黄酮的变化。因此,氯化钾可作为一种有价值的食品加工辅助剂来提高食品材料的生物活性。
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引用次数: 0
Effect of temperature on the quality and microbial community during Daocai fermentation 温度对大白菜发酵过程中的质量和微生物群落的影响
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-24 DOI: 10.1016/j.fochx.2024.101827
Xueli Wang , Xueting Liu , Chunmei Sun , Yanwei Cheng , Zhen Li , Shuyi Qiu , Yongguang Huang
Daocai is a traditional salted pickle in the southeastern region of Guizhou with a unique aroma, color, and taste. The quality of Daocai is greatly influenced by the fermentation temperature. In this study, high-throughput sequencing and headspace-gas chromatography-ion mobility spectrometry were used to investigate the changes in microbial community succession and volatile flavor compounds during Daocai fermentation under temperature-controlled (D group) and non-temperature-controlled (C group).We found that the predominant genera in the C group samples were Latilactobacillus(40.57 %), Leuconostoc(21.25 %), Cystofilobasidium(22.12 %), Vishniacozyma(23.89 %), and Leucosporidium(24.95 %), whereas Weissella(29.39 %), Lactiplantibacillus(45.61 %), Mucor(68.26 %), and Saccharomyces(23.94 %) were the predominant genera in the D group. A total of 92 VFCs were detected in Daocai samples, including 5 isothiocyanates, 16 esters, 14 alcohols, 24 aldehydes, 17 ketones, 3 acids, 2 pyrazines, 1 pyridines, 1 thiazoles, 3 furans, 4 alkenes, and 2 nitriles. Further analysis revealed Latilactobacillus, Leuconostoc, Lactococcus, Cystofilobasidium, Leucosporidium, Holtermanniella, and Dioszegia as key bacteria involved in flavor formation. They are closely related to the formation of flavors such as aldehydes, furans, pyridines, and alkenes. This study contributes to our understanding of the relationship between bacterial communities and the flavor formation during Daocai fermentation.
道菜是贵州东南部地区的一种传统咸菜,具有独特的香气、色泽和口感。发酵温度对道菜品质的影响很大。本研究采用高通量测序和顶空气相色谱-离子迁移谱法研究了控温(D组)和非控温(C组)条件下道菜发酵过程中微生物群落演替和挥发性风味化合物的变化。我们发现,C 组样品中的优势菌属为拉氏乳杆菌(40.57 %)、白念珠菌(21.25 %)、子囊菌(22.12 %)、维氏乳杆菌(23.而 D 组的主要菌属为魏氏菌(29.39 %)、乳杆菌(45.61 %)、粘菌(68.26 %)和酵母菌(23.94 %)。在道才样本中总共检测到 92 种 VFCs,包括 5 种异硫氰酸盐、16 种酯、14 种醇、24 种醛、17 种酮、3 种酸、2 种吡嗪、1 种吡啶、1 种噻唑、3 种呋喃、4 种烯和 2 种腈纶。进一步分析发现,拉氏乳杆菌、白念珠菌、乳球菌、囊丝菌、白孢子菌、霍尔特曼菌和薯蓣皂苷菌是参与风味形成的主要细菌。它们与醛、呋喃、吡啶和烯类等风味的形成密切相关。这项研究有助于我们了解大白菜发酵过程中细菌群落与风味形成之间的关系。
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引用次数: 0
Effect and mechanism of green and aldehyde aroma compounds from sweet orange on sucrose sweetness perception 甜橙中的绿色和醛类香气化合物对蔗糖甜度感知的影响及其机理
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-23 DOI: 10.1016/j.fochx.2024.101853
ZuoBing Xiao , HouWang Wang , YunWei Niu , JianCai Zhu , Yamin Yu , YuanBin She , RuJun Zhou , Zhaogai Wang , Jing Zhang
At present, there are relatively few studies on the influence of green aroma and aldehyde aroma compounds on the sweetness perception of sucrose. This study examined the effects of 11 aroma compounds from sweet orange, characterized by green and aldehyde flavors, on the sweetness of a 5 % sucrose solution. Using artificial sensory analysis and electronic tongue technology, it was found that most aromatic compounds can inhibit sweetness perception, and the inhibitory effect of trans-2-decenoaldehyde is the most significant. The mechanism of inhibition was explored through molecular simulation, revealing that the binding free energy of molecular docking was greater than −5.9 kcal/mol. Further molecular dynamics analysis showed that compared with the T1R2/T1R3 sucrose binary system, the addition of aroma substances reduced the number of hotspot residues involved in protein ligand binding, and did not enhance the binding ability of ligand proteins, indicating an inhibitory effect.
目前,有关绿色香气和醛类香气化合物对蔗糖甜度感知影响的研究相对较少。本研究考察了以绿色和醛香味为特征的 11 种甜橙芳香化合物对 5% 蔗糖溶液甜度的影响。通过人工感官分析和电子舌技术发现,大多数芳香化合物都能抑制甜味感知,其中反式-2-癸烯醛的抑制作用最为显著。通过分子模拟探讨了抑制机制,发现分子对接的结合自由能大于-5.9 kcal/mol。进一步的分子动力学分析表明,与 T1R2/T1R3 蔗糖二元体系相比,香气物质的加入减少了参与蛋白质配体结合的热点残基数量,并没有增强配体蛋白质的结合能力,表明其具有抑制作用。
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引用次数: 0
Unraveling Shengmuxiang in Jiang-flavor base baijiu using a combination of metabolomics and sensomics strategy 利用代谢组学和感官组学相结合的策略,揭示江味基酒中的圣木香
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-21 DOI: 10.1016/j.fochx.2024.101851
Yang Xu, Lizhang Yang, Yubo Yang, Fan Yang
Shengmuxiang (SMX), an important aroma in Jiang-flavor base baijiu, significantly influences the quality of the product. This study employed untargeted metabolomics combined with sensomics to explore the key compounds responsible for SMX. Results indicated that SMX samples had higher intensities of green and woody-like odors compare to control samples. A total of 87 aroma compounds were identified by headspace solid phase microextraction combined with gas chromatography–mass spectrometry technology. Based on the variable projection importance, PCA and OPLS-DA were employed to identify 22 potential marker compounds. Quantitative results combined with hierarchical cluster and OAV analysis revealed that 9 aroma compounds (OAV > 1) had high concentrations in SMX samples. Aroma recombination and omission experiments further indicated that acetaldehyde and acetal were the key compounds responsible for the characteristic aroma of SMX in Jiang-flavor base baijiu. These findings provide valuable insights into the distinct aroma profile of SMX and offer a basis for quality control of Jiang-flavor base baijiu.
生木香(SMX)是江味基白酒中的一种重要香味,对产品的品质有很大影响。本研究采用非靶向代谢组学与感官组学相结合的方法,探究产生生香的关键化合物。结果表明,与对照样品相比,SMX 样品具有更高强度的绿色和木香气味。顶空固相微萃取与气相色谱-质谱联用技术共鉴定出 87 种芳香化合物。根据变量预测的重要性,采用 PCA 和 OPLS-DA 方法确定了 22 种潜在的标记化合物。结合分层聚类和 OAV 分析的定量结果显示,9 种香气化合物(OAV > 1)在 SMX 样品中具有较高的浓度。香气重组和遗漏实验进一步表明,乙醛和乙缩醛是造成江味基酒中 SMX 特有香气的关键化合物。这些发现为了解 SMX 的独特香气特征提供了宝贵的见解,并为江味基酒的质量控制提供了依据。
{"title":"Unraveling Shengmuxiang in Jiang-flavor base baijiu using a combination of metabolomics and sensomics strategy","authors":"Yang Xu,&nbsp;Lizhang Yang,&nbsp;Yubo Yang,&nbsp;Fan Yang","doi":"10.1016/j.fochx.2024.101851","DOIUrl":"10.1016/j.fochx.2024.101851","url":null,"abstract":"<div><div>Shengmuxiang (SMX), an important aroma in Jiang-flavor base baijiu, significantly influences the quality of the product. This study employed untargeted metabolomics combined with sensomics to explore the key compounds responsible for SMX. Results indicated that SMX samples had higher intensities of green and woody-like odors compare to control samples. A total of 87 aroma compounds were identified by headspace solid phase microextraction combined with gas chromatography–mass spectrometry technology. Based on the variable projection importance, PCA and OPLS-DA were employed to identify 22 potential marker compounds. Quantitative results combined with hierarchical cluster and OAV analysis revealed that 9 aroma compounds (OAV &gt; 1) had high concentrations in SMX samples. Aroma recombination and omission experiments further indicated that acetaldehyde and acetal were the key compounds responsible for the characteristic aroma of SMX in Jiang-flavor base baijiu. These findings provide valuable insights into the distinct aroma profile of SMX and offer a basis for quality control of Jiang-flavor base baijiu.</div></div>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"24 ","pages":"Article 101851"},"PeriodicalIF":6.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preparation and characterization of intelligent packaging labels based on pea starch, κ-carrageenan and black raspberry extract for monitoring freshness of pork 基于豌豆淀粉、κ-卡拉胶和黑树莓提取物的智能包装标签的制备和表征,用于监测猪肉新鲜度
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-21 DOI: 10.1016/j.fochx.2024.101850
Changxing Jiang , Gang Liu , Qian Zhang , Siyu Wang , Yufei Zou
Intelligent biodegradable packaging has gained significant attention recently due to its great potential for food freshness monitoring. In this study, we developed intelligent labels using pea starch (PS), κ-carrageenan (KC) and black raspberry extract (BRE) and evaluated their physicochemical properties and effectiveness in pork freshness monitoring. Incorporation of KC significantly decreased tensile strength (TS) (33.43 to 19.84 MPa) and increased elongation at break (EAB) (6.26 to 7.41 %) and water vapor permeability (WVP) (2.11 to 2.48 × 10−9 g m−1 s−1 Pa−1) of PS label. Incorporation of BRE significantly decreased WVP (2.48 to 1.96 × 10−9 g m−1 s−1 Pa−1) and increased TS (19.84 to 29.68 MPa) and EAB (7.41 to 11.09 %) of PS-KC label. PS-KC-BRE labels showed increased light barrier performance, thermal stability, antioxidant activity, pH and ammonia sensitivities, and significant visible color change as pH indicators. Findings suggest PS-KC-BRE labels can be used as intelligent packaging in food industry.
智能生物降解包装因其在食品新鲜度监测方面的巨大潜力而在近期受到广泛关注。在这项研究中,我们使用豌豆淀粉(PS)、κ-卡拉胶(KC)和黑覆盆子提取物(BRE)开发了智能标签,并评估了它们的理化特性和在猪肉新鲜度监测中的有效性。加入 KC 后,PS 标签的拉伸强度(TS)明显降低(33.43 至 19.84 兆帕),断裂伸长率(EAB)(6.26 至 7.41 %)和水蒸气渗透率(WVP)(2.11 至 2.48 × 10-9 g m-1 s-1 Pa-1)明显增加。BRE 的加入明显降低了 PS-KC 标签的 WVP(2.48 至 1.96 × 10-9 g m-1 s-1 Pa-1),增加了 TS(19.84 至 29.68 MPa)和 EAB(7.41 至 11.09 %)。PS-KC-BRE 标签的光阻隔性能、热稳定性、抗氧化活性、pH 值和氨敏感性都有所提高,而且作为 pH 值指示剂的可见颜色变化显著。研究结果表明,PS-KC-BRE 标签可用作食品工业的智能包装。
{"title":"Preparation and characterization of intelligent packaging labels based on pea starch, κ-carrageenan and black raspberry extract for monitoring freshness of pork","authors":"Changxing Jiang ,&nbsp;Gang Liu ,&nbsp;Qian Zhang ,&nbsp;Siyu Wang ,&nbsp;Yufei Zou","doi":"10.1016/j.fochx.2024.101850","DOIUrl":"10.1016/j.fochx.2024.101850","url":null,"abstract":"<div><div>Intelligent biodegradable packaging has gained significant attention recently due to its great potential for food freshness monitoring. In this study, we developed intelligent labels using pea starch (PS), κ-carrageenan (KC) and black raspberry extract (BRE) and evaluated their physicochemical properties and effectiveness in pork freshness monitoring. Incorporation of KC significantly decreased tensile strength (TS) (33.43 to 19.84 MPa) and increased elongation at break (EAB) (6.26 to 7.41 %) and water vapor permeability (WVP) (2.11 to 2.48 × 10<sup>−9</sup> g m<sup>−1</sup> s<sup>−1</sup> Pa<sup>−1</sup>) of PS label. Incorporation of BRE significantly decreased WVP (2.48 to 1.96 × 10<sup>−9</sup> g m<sup>−1</sup> s<sup>−1</sup> Pa<sup>−1</sup>) and increased TS (19.84 to 29.68 MPa) and EAB (7.41 to 11.09 %) of PS-KC label. PS-KC-BRE labels showed increased light barrier performance, thermal stability, antioxidant activity, pH and ammonia sensitivities, and significant visible color change as pH indicators. Findings suggest PS-KC-BRE labels can be used as intelligent packaging in food industry.</div></div>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"24 ","pages":"Article 101850"},"PeriodicalIF":6.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of foam-mat drying conditions of “Gấc” aril on drying rate and bioactive compounds: Optimization by novel statistical approaches Gấc "假种皮的泡沫垫干燥条件对干燥速率和生物活性化合物的影响:通过新型统计方法进行优化
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-20 DOI: 10.1016/j.fochx.2024.101849
Nguyen Minh Thuy , Vo Quoc Tien , Tran Ngoc Giau , Hong Van Hao , Vo Quang Minh , Ngo Van Tai
This study was conducted to optimize the foam-mat drying conditions to maximize quality [β-carotene and total polyphenol content (TPC)] and drying rate of “Gấc” aril powder by using two novel statistical techniques as Response Surface Methodology (RSM) and Artificial Neural Network (ANN) couple with Genetic Algorithm (GA). During production process, level of egg albumin (EA) used for foaming process and drying temperature mainly influenced the drying rate and content of antioxidant compounds in powder. ANN model of 3–10–3 showed more accuracy and faster prediction capacity than RSM model did. ANN-GA model predicted the optimal conditions to be 13.31 % EA, 0.26 % xanthan gum and drying temperature of 73.1 °C, with the drying rate of 1.89 g-water/g-dry matter/min, β-carotene content of 395.88 μg/g, TPC of 1.68 mgGAE/g. These results confirmed the suitability and promising of foam-mat drying for “Gấc” aril powder production, to be producing food ingredient containing highly bioactive compounds.
本研究采用响应面方法学(RSM)和人工神经网络(ANN)与遗传算法(GA)相结合的两种新型统计技术,对泡沫垫干燥条件进行了优化,以最大限度地提高 "猕猴桃 "假种皮粉的质量[β-胡萝卜素和总多酚含量(TPC)]和干燥速率。在生产过程中,用于发泡的鸡蛋白蛋白(EA)含量和干燥温度主要影响干燥速率和粉末中抗氧化化合物的含量。与 RSM 模型相比,3-10-3 的 ANN 模型显示出更高的准确性和更快的预测能力。ANN-GA 模型预测的最佳条件为 13.31 % 的 EA、0.26 % 的黄原胶和 73.1 ℃ 的干燥温度,干燥速率为 1.89 g-水/g-干物质/min,β-胡萝卜素含量为 395.88 μg/g,TPC 为 1.68 mgGAE/g。这些结果证实了泡沫垫干燥法适用于 "猕猴桃 "假种皮粉的生产,并有望生产出含有高生物活性化合物的食品配料。
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引用次数: 0
Unveils key proteins in Xinjiang goat muscle linked to post-mortem meat quality: A TMT-based proteomic analysis 揭示新疆山羊肌肉中与死后肉质有关的关键蛋白质基于TMT的蛋白质组分析
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-20 DOI: 10.1016/j.fochx.2024.101847
Duoduo Zhang , Hong Yu , Minghui Gu , Shiquan Zhang , Xiaolin Ma , Wei Zhang , Yanlei Zhu , Majida Al-Wraikat , Mohamed Aamer Abubaker , Rui Zhang , Yongfeng Liu
An extensive proteomic analysis utilizing the tandem mass tag (TMT) method was conducted to investigate the changes in protein expression in the longissimus dorsi muscle of Xinjiang goats over various post-mortem intervals: immediately after death within 0 h, 12 h, 24 h and 48 h. The investigation carefully identified around 108 proteins that showed significant changes in expression during these intervals. Among these proteins, six were highlighted for their crucial roles in muscle growth and differentiation of muscle fibers post-mortem. These proteins, namely COL12A1, MRPL46, CTNNB1, MYH1, CAPZA1, and MYL9, have a direct effect on the meat's quality attributes, such as tenderness and color. Further discuss observed a progressive increase in the expression of proteins linked with oxidative metabolism (MSRB2, ENOX1, LOC102170282, GSTM1, and AOC3) as the post-mortem aging period extended, particularly between 24 h to 48 h. These proteins are instrumental in defining the color and flavor profiles of goat meat, underscoring the importance of precise processing and storage conditions to preserve meat quality during the critical aging phase. This enhanced understanding of protein expression dynamics offers significant implications for optimizing meat quality and provides a scientific basis for post-mortem handling practices in the goat meat industry.
利用串联质量标记(TMT)方法对新疆山羊背长肌进行了广泛的蛋白质组学分析,研究了山羊死后不同时间段(死后0 h、12 h、24 h和48 h)蛋白质表达的变化情况。在这些蛋白质中,有六种因其在肌肉生长和死后肌纤维分化中的关键作用而受到关注。这些蛋白质(即 COL12A1、MRPL46、CTNNB1、MYH1、CAPZA1 和 MYL9)对肉的品质属性(如嫩度和颜色)有直接影响。进一步的讨论发现,随着死后陈化时间的延长,特别是在 24 小时到 48 小时之间,与氧化代谢有关的蛋白质(MSRB2、ENOX1、LOC102170282、GSTM1 和 AOC3)的表达量逐渐增加。加强对蛋白质表达动态的了解对优化肉质具有重要意义,并为山羊肉行业的死后处理方法提供了科学依据。
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引用次数: 0
Polyphenol co-pigments enhanced the antioxidant capacity and color stability of blue honeysuckle juice during storage 多酚辅助色素增强了蓝金银花汁在贮藏期间的抗氧化能力和颜色稳定性
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-09-20 DOI: 10.1016/j.fochx.2024.101848
Yifan Geng , Kaojia Cui , Na Ding , Houping Liu , Junwei Huo , Xiaonan Sui , Yan Zhang
The study aimed to assess the impact of incorporating five co-pigments (gallic acid, quercetin, rutin, catechin, and epigallocatechin gallate (EGCG)) on the color stability of blue honeysuckle juice (BHJ). Additionally, it sought to determine the influence of varying proportions of anthocyanins in an accelerated test (light at 40 °C for 24 d). Results indicated that the addition of polyphenol co-pigments effectively mitigated the thermal degradation of anthocyanins, enhancing color saturation and antioxidant capacity of BHJ. Notably, quercetin, rutin, catechin, and EGCG exhibited superior efficacy compared to gallic acid. FTIR analysis revealed non-covalent complex formation between co-pigments and anthocyanins, including hydrogen bonds and van der Waals forces, thereby shielding them from degradation. HPLC-ESI-QTOF-MS2 identified 15 anthocyanins and 39 non-anthocyanin polyphenols. Addition of co-pigments effectively curbed anthocyanin degradation, thus stabilizing juice system. Consequently, judicious incorporation of co-pigments holds promise as a technology for enhancing the color quality and stability of BHJ during processing.
该研究旨在评估加入五种辅助色素(没食子酸、槲皮素、芦丁、儿茶素和表没食子儿茶素没食子酸酯(EGCG))对蓝色金银花汁(BHJ)颜色稳定性的影响。此外,该研究还试图确定不同比例的花青素在加速试验(40 °C 下光照 24 d)中的影响。结果表明,添加多酚辅助颜料可有效缓解花青素的热降解,提高 BHJ 的颜色饱和度和抗氧化能力。值得注意的是,与没食子酸相比,槲皮素、芦丁、儿茶素和 EGCG 表现出更高的功效。傅立叶变换红外光谱分析显示,辅颜料和花青素之间形成了非共价复合物,包括氢键和范德华力,从而保护了它们免受降解。HPLC-ESI-QTOF-MS2 鉴定出 15 种花青素和 39 种非花青素多酚。添加辅颜料可有效抑制花青素降解,从而稳定果汁体系。因此,在加工过程中明智地加入助颜料有望成为一种提高 BHJ 颜色质量和稳定性的技术。
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引用次数: 0
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