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Dynamics of linalool and its derivatives enantiomers in Camellia sinensis var. A ssamica "Hainan dayezhong". 海南大叶中茶树中芳樟醇及其衍生物对映体的动态变化。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-20 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102109
Ying Zhou, Junjie Tian, Hainuo Hong, Yang Gao, Yunchuan He, Zeng-Rong Zhu

We here analyzed changes in the proportion and content of chiral isomers of linalool and its derivatives in "Hainan dayezhong" throughout its life cycle from tea tree growth and tea manufacturing to brewing. The chiral isomers of aromatic compounds present in fresh tea leaves were found to undergo substantial diurnal and seasonal changes during tea tree growth, and their proportions varied slightly across different leaf positions. The chiral isomer content of linalool and its derivatives was consistently higher in stems than in leaves. Pest and disease stress significantly increased the proportion and content of type-R aroma. The proportion of chiral isomers underwent no considerable change during black tea manufacturing. However, their content varied dramatically among different processes. Diversity in the proportion and content of chiral isomers was observed in the wild tea tree. Further research should focus on breeding "Hainan dayezhong" wild resources to generate clones with high aroma quality.

本文分析了“海南大叶中”从茶树生长到制茶到冲泡整个生命周期中芳樟醇及其衍生物手性异构体的比例和含量的变化。新鲜茶叶中芳香化合物的手性异构体在茶树生长过程中发生了明显的昼夜和季节变化,其比例在不同叶位之间略有不同。芳樟醇及其衍生物的手性异构体含量在茎中始终高于在叶中。病虫害胁迫显著提高了r型香气的比例和含量。手性异构体的比例在红茶制造过程中没有明显的变化。然而,它们的内容在不同的过程中差异很大。野生茶树手性异构体的比例和含量存在差异。进一步的研究应集中于培育“海南大叶中”野生资源,以获得高香气品质的无性系。
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引用次数: 0
Nutritional and antioxidative characterization, antimicrobial and sensorial stability of flaxseed powder supplemented mutton patties. 亚麻籽粉添加羊肉肉饼的营养和抗氧化特性、抗菌和感官稳定性。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-20 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102098
Raheel Suleman, Hira Choudhary, Muhammad Waseem, Jaza Maqbl Alshammari, Muhammad Muzamil, Huan Liu, Tariq Ismail, Muhammad Ammar Khan, Muhammad Qamar, Crossby Osei Tutu

Oxidative stress and microbial growth deteriorate food quality and cause safety risks. Therefore, the present study was investigated to explore the nutritional, sensorial, anti-oxidative and anti-microbial attributes of flaxseed powder (FP) supplemented at 2-8 % (i.e., T1-T4) level in mutton patties. Among extracts, the aqueous flaxseed extracts exhibited the highest total phenolic contents (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) activities i.e., 275 mg GAE/g, 75 % and 57 mg/g, respectively. The results exhibited significant increase (p < 0.05) in protein, and ash contents from 10.7 to 20.6 and 1.3-2.4 (g/100 g) on addition of FP at 2-8 % in mutton patties, respectively. Likewise, the mutton patties exhibited significant (p < 0.05) decrease in pH and thiobarbituric acid reactive substances (TBARS) of T1-T4 from 7.1 to 3.4 and 317-46 than control i.e., 6.64-4.12 and 453.75-563 at 0-16 days. Among color of mutton patties, L values decreased from 37 to 25, while, b values increased from 10 to 13 on addition of 2-8 % FP in mutton patties at 0-16 days for T1-T4, respectively. A significant decrease (p < 0.05) in total plate counts (TPC), Salmonella and E. coli of mutton patties was recorded in T0-T4 from 4.2 to 2.8, 0.8-0.3, and 2.1-0.3, respectively at 16th day of storage, respectively. Sensory experts rated the T1 (i.e., 2 % FP supplemented mutton patties) owing to their best sensory scores and overall acceptability. Conclusively, owing to better potential of FP in improving the nutritional and microbial quality of mutton patties, the study further suggests its ability to act as a novel functional ingredient of choice in shelf-life extension of other food products.

氧化应激和微生物生长使食品质量恶化,并造成安全风险。因此,本研究旨在探讨在羊肉肉饼中添加2- 8%(即T1-T4)水平的亚麻籽粉(FP)的营养、感官、抗氧化和抗微生物特性。其中,亚麻仁水提液的总酚含量(TPC)、2,2-二苯基-1-苦味酰腙(DPPH)和铁还原抗氧化能力(FRAP)活性最高,分别为275 mg GAE/g、75%和57 mg/g。结果表明,添加2- 8%的FP显著提高了羊肉饼蛋白质和灰分含量(p 0.05),分别从10.7 ~ 20.6 (g/100 g)和1.3 ~ 2.4 (g/100 g)。在0-16 d,肉饼的p 1-T4值在7.1 ~ 3.4和317 ~ 46显著高于对照,分别为6.64 ~ 4.12和453.75 ~ 563。在肉饼颜色中,添加2- 8% FP的肉饼在T1-T4的0-16天,L值分别从37下降到25,b值从10上升到13。贮藏第16天,t0 ~ t4期羊肉肉饼的总平板计数(TPC)、沙门氏菌数和大肠杆菌数分别从4.2降至2.8、0.8 ~ 0.3和2.1 ~ 0.3显著降低(p 0.05)。感官专家对T1(即添加2% FP的羊肉肉饼)进行了评级,因为它们的感官得分最高,总体可接受度也最高。综上所述,由于FP在改善羊肉肉饼的营养和微生物品质方面具有更好的潜力,该研究进一步表明,它有能力作为一种新的功能成分,用于延长其他食品的保质期。
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引用次数: 0
Nutritional health aspects and functional properties of nut yogurt: Future perspectives. 坚果酸奶的营养健康方面和功能特性:未来展望。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-20 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102102
Jiangxia Zhai, Yongliang Zhuang, Liping Sun, Ying Gu, Xuejing Fan

The increase in lactose intolerance, the rise of veganism, the pursuit of healthy lifestyles, environmental awareness and concern for animal welfare have led to an increase in consumer demand for plant-based yogurts. The high nutritional value of nuts makes them an ideal ingredient for the production of plant-based yogurts. The main challenge for such products is to achieve a similar taste to traditional yogurt while improving shelf life. In recent years, extensive research has been conducted on this topic. The nutritional and health properties of yogurts made from different types of nuts, traditional and innovative processing technologies, and the effects of fermentation on the nutritional value, sensory characteristics, and texture of the yogurts are described. This review provides a comprehensive overview of the nutritional and manufacturing process of nut yogurts and offers possible directions for development and innovation of health food products.

乳糖不耐症的增加、素食主义的兴起、对健康生活方式的追求、环保意识和对动物福利的关注,导致消费者对植物性酸奶的需求增加。坚果的高营养价值使它们成为生产植物性酸奶的理想成分。这类产品面临的主要挑战是在延长保质期的同时保持与传统酸奶相似的口感。近年来,对这一主题进行了广泛的研究。介绍了由不同种类坚果制成的酸奶的营养和保健特性、传统和创新的加工技术,以及发酵对酸奶营养价值、感官特性和质地的影响。本文综述了坚果酸奶的营养成分和生产工艺,为保健食品的开发和创新提供了可能的方向。
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引用次数: 0
Structural and functional properties of fava bean albumin, globulin and glutelin protein fractions. 蚕豆白蛋白、球蛋白和谷蛋白组分的结构和功能特性。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-20 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102104
Timilehin David Oluwajuyitan, Rotimi Emmanuel Aluko

This study reports a comparative evaluation of the physicochemical and functional properties of fava bean albumin, globulin and glutelin proteins. The fava bean globulins had significantly (p < 0.05) higher protein content (88.49 %) than the albumin (83.47 %) and glutelin (86.71 %). Far-UV circular dichroism results indicate low contents of α-helix, but high levels of unordered and β-sheet structures in the albumin and globulin. Higher surface hydrophobicity of the globulins was directly related to formation of oil-in-water emulsions with smaller oil droplet sizes, and better foaming capacity than the albumin and glutelin. The albumin had a broad range (32-92 %) of protein solubility that covers acidic and alkaline pH while glutelin exhibited significantly higher in vitro protein digestibility (77.33 %) when compared to the 75.34 and 71.73 % for globulin and albumin, respectively. We conclude that each fava bean protein fraction may find specific uses as ingredients for the formulation of various food products.

本研究报道了蚕豆白蛋白、球蛋白和谷蛋白的理化性质和功能特性的比较评价。蚕豆球蛋白含量显著高于(p
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引用次数: 0
Characterization of phospholipid profiles of egg yolks: Newly classified plasmalogens, distribution of polyunsaturated fatty acids, and the effects of dietary enrichment. 蛋黄磷脂谱的表征:新分类的磷脂原,多不饱和脂肪酸的分布,以及膳食富集的影响。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-19 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102105
Le Cheng, Xinlei Yuan, Ming Zhang, Jianguo Dong, Yao Wu, Rang Wang, Yixuan Li, Lishui Chen, Bing Fang

Egg yolk phospholipids are commercially valuable products that are beneficial to human health. Previous research on phospholipids in egg yolk mainly focuses on phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), and fatty acid compositions, and neglects the esterification position and other bioactive phospholipids. This study found a total of 19 classes of phospholipids and 275 subclasses using lipidomics. The study firstly found that egg yolks were also rich in glucosylceramides, galactosylceramides, lactosylceramides, gangliosides, and plasmalogens with polyunsaturated fatty acids (PUFAs) at the high bioavailable sn-2 position. Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), α-Linolenic acid (ALA), and arachidonic acid (ARA) were esterified at sn-1 position of PC and sn-2 position of PE, phosphatidyl inositol (PI) and phosphatidic acid (PA). Microalgae feeding contributed to the deposition of PUFAs at sn-2 position and increased the contents of plasmalogens. The results provided detail the phospholipid profiles of egg yolk to improve understanding of its nutrition.

蛋黄磷脂是有益人体健康的具有商业价值的产品。以往对蛋黄磷脂的研究主要集中在磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)和脂肪酸组成上,而忽略了酯化位置和其他生物活性磷脂。本研究使用脂质组学方法共发现了19类磷脂和275个亚类磷脂。本研究首次发现,蛋黄中还含有丰富的葡萄糖神经酰胺、半乳糖神经酰胺、乳糖神经酰胺、神经节苷类,以及具有高生物利用度sn-2位置的多不饱和脂肪酸(PUFAs)的聚脂原。二十二碳六烯酸(DHA)、二十碳五烯酸(EPA)、α-亚麻酸(ALA)和花生四烯酸(ARA)分别在PC的sn-1位置和PE、磷脂酰肌醇(PI)和磷脂酸(PA)的sn-2位置酯化。微藻的摄食促进了PUFAs在sn-2位点的沉积,提高了磷脂原的含量。结果提供了详细的蛋黄磷脂谱,以提高对其营养的认识。
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引用次数: 0
Ultrasound pretreatment and solvent extraction parameters effects on the nutritional characteristics of Indonesian shortfin eel (Anguilla bicolor bicolor) protein concentrate. 超声预处理和溶剂提取参数对印尼短鳍鳗浓缩蛋白营养特性的影响。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-19 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102103
Andri Cahyo Kumoro, Dyah Hesti Wardhani, Tutuk Djoko Kusworo, Mohamad Djaeni, Yusuf Ma'rifat Fajar Azis, Misbahudin Alhanif, Tan Chin Ping

Protein concentrate (PC) is a potential solution to address the global protein shortage, with Indonesian shortfin eel being a suitable raw material. This research investigates the impact of ultrasound pretreatment and extraction parameters on the nutritional quality of eel protein concentrate (EPC). The study involved ultrasonic pretreatment at different times and power, and solvent extraction with different solvents, temperature, and solvent-solid-feed-ratio (SSFR). The results showed that the recommended conditions for EPC preparation were a mixture of ethanol-hexane, ultrasonic pretreatment at 250 W for 25 min, extraction temperature and SSFR of 40 °C and 6:1 v/w. The protein content of EPC increased gradually with the increase of SSFR until it reached a ratio of 6:1, further increase in SSFR promoted the development of a pseudo-homogeneous system, leading to a reduction in the solvent-eel flesh contact and the relative velocity between the extracting solvent and eel flesh, and consequently decreased the extraction yield. The prepared EPC is classified as type B EPC, with a protein content of 89.62 %w.b. and a lipid content of 2.21 %w.b. The EPC contains five types of peptides with a molecular weight of 5.00-76.00 kDa, with the main fraction having a MW ranging from 10.00 to 15.00 kDa, indicating potential for functional food products.

浓缩蛋白(PC)是解决全球蛋白质短缺的潜在解决方案,印尼短鳍鱼是一种合适的原料。研究了超声预处理和提取工艺对鳗鱼浓缩蛋白营养品质的影响。研究包括不同时间和功率的超声预处理,不同溶剂、温度和溶剂固料比(SSFR)下的溶剂萃取。结果表明,EPC制备的最佳工艺条件为乙醇-己烷混合,超声预处理250 W,预处理25 min,提取温度40℃,SSFR为6:1 v/ W。随着SSFR的增加,EPC蛋白含量逐渐增加,达到6:1的比例,SSFR的进一步增加促进了伪均相体系的发展,导致溶剂与鳗鱼肉的接触减少,提取溶剂与鳗鱼肉的相对速度降低,从而降低了提取收率。所得EPC为B型EPC,蛋白质含量为89.62%。脂质含量为2.21%。EPC包含5种分子量在5.00-76.00 kDa之间的肽,其中主要部分分子量在10.00 - 15.00 kDa之间,具有功能性食品的潜力。
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引用次数: 0
Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness. 在线监测肉类新鲜度的胺响应荧光比例传感器阵列的按需打印。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-18 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102099
Zhijian Wang, Xudong Shi, Jingze Guo, Lin Wang, Meilin Cao, Shiyao Wang, Yisheng Chen

Aiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH3) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and uniformly printed with fluorescein isothiocyanate (FITC) as indicator and rhodamine B (RhB) as internal reference on the filter-paper, respectively. The fluorescence intensity of the array area was calibrated according to Green/Red ratio of the digitalized pixels extracted from images facilitated by a smartphone. The fluorescence-ratiometric sensor array displayed remarkable detection performances, including high sensitivity (LOD = 1.1 ppm), stability (91 % responding attenuation over 10 d of storage) and reproducibility (RSD < 10 %), which was further validated with real pork and shrimp samples. Subsequently, the fluorescent signals of the dual-rectangle array showed high correlation to the total volatile base nitrogen value that was officially used for indexing the meat freshness status.

为了实现对空气包装肉类的在线新鲜度监测,我们开发了一种比率测量阵列,该阵列对蛋白质腐烂释放的挥发性有机化合物氨(NH3)具有荧光响应。该阵列由两个3mm × 6mm的矩形组成,分别以异硫氰酸荧光素(FITC)为指示剂,罗丹明B (RhB)为内参,精确均匀地打印在滤纸上。阵列区域的荧光强度根据从智能手机图像中提取的数字化像素的绿/红比进行校准。荧光比率传感器阵列显示出卓越的检测性能,包括高灵敏度(LOD = 1.1 ppm),稳定性(在10天的存储时间内响应衰减91%)和重复性(RSD)
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引用次数: 0
Characterization and comparative analysis of volatile organic compounds in four aromatic wild strawberry species using HS-SPME-GC-MS. 采用HS-SPME-GC-MS对4种野生芳香草莓挥发物进行了表征和比较分析。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-18 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102092
Linlin Xu, Wan Liu, Zhiliang Pan, Fuhua Pang, Yongqi Zhang, Jiahui Liang, Qinglian Wang, Jing Wang, Mizhen Zhao, Yushan Qiao, Huazhao Yuan

Strawberries are valued for their aroma, which is mainly determined by volatile organic compounds (VOCs). Wild strawberries, with broader and more intense VOC profiles, are especially important in breeding programs. Using HS-SPME-GC-MS, 126 VOCs were identified in the ripe fruit of 22 cultivars from four wild strawberry species. Significant interspecies differences were found, with Fragaria mandshurica and F. nilgerrensis showing several times higher VOC levels than F. vesca and F. viridis, primarily due to high lactone content (up to 79.71 % of total VOCs). Phylogenetic analysis revealed conserved VOC profiles within species and genotype-aroma correlations. PCA and PLS-DA identified 60 biomarkers explaining 37.07 % of the variance, with 10 biomarkers validated for species identification, achieving 100 % accuracy. Key biomarkers for F. mandshurica included furaneol and perillyl acetate, while butanoic acid butyl ester was characteristic of F. vesca. This study emphasizes the role of VOCs as biomarkers for species differentiation and their potential in strawberry breeding.

草莓的香气主要由挥发性有机化合物(VOCs)决定。野生草莓具有更广泛和更强烈的挥发性有机化合物,在育种计划中尤为重要。利用HS-SPME-GC-MS,从4个野生草莓品种的22个品种的成熟果实中鉴定出126种挥发性有机化合物。不同种间的挥发性有机化合物(VOC)含量差异显著,水曲柳和nilgerrensis的挥发性有机化合物(VOC)含量比水曲柳和青枝高几倍,主要是由于其内酯含量较高(占总挥发性有机化合物的79.71%)。系统发育分析显示,VOC在物种内具有保守性,且基因型与香气具有相关性。PCA和PLS-DA鉴定了60个生物标记物,解释了37.07%的方差,其中10个生物标记物被证实可用于物种鉴定,准确率达到100%。水曲霉的关键生物标志物为呋喃醇和紫苏酯,而水曲霉的特征生物标志物为丁酸丁酯。本研究强调了挥发性有机化合物作为物种分化生物标志物的作用及其在草莓育种中的潜力。
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引用次数: 0
Optimization of bioactive compounds and sensory quality in thermosonicated black carrot juice: A study using response surface methodology, gradient boosting, and fuzzy logic. 热超声黑胡萝卜汁生物活性成分及感官品质优化:响应面法、梯度增强和模糊逻辑研究。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-15 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102096
Seydi Yıkmış, Melikenur Türkol, Ishak Pacal, Aylin Duman Altan, Nazlı Tokatlı, Gholamreza Abdi, Nazan Tokatlı Demirok, Rana Muhammad Aadil

This study investigates the optimization of bioactive components in thermosonicated black carrot juice using response surface methodology (RSM) and gradient boosting (GB) modeling techniques. Thermosonication, a combination of ultrasound and heat, was applied to enhance the nutritional quality of black carrot juice, which is rich in anthocyanins, phenolic compounds, and antioxidants. The study examined the effects of temperature, processing time, and ultrasonic amplitude on total carotenoid content (TCC), total anthocyanin content (TAC), ferric reducing antioxidant power (FRAP), and total phenolic content. RSM demonstrated higher prediction accuracy compared to GB, identifying optimal processing conditions at 48.68 °C, 11.15 minutes, and 82.62% amplitude. Thermosonication significantly increased total phenolic content to 414.28 mg GAE/L, surpassing traditional pasteurization. Sensory analysis, conducted via fuzzy logic, indicated improved sensory properties, including aroma, taste, and color, in thermosonicated samples. This study undercomes thermosonication as a promising method for improving both bioactive compounds and sensory quality in black carrot juice.

Chemical compounds: Chlorogenıc acid (PubChem CD:1794427); caffeic acid (PubChem CD: 689043); vanillin (PubChem CD: 1183); rutin (PubChem CD: 5280805); naringin (PubChem CD: 442428); rosmarinic acid (PubChem CD: 5281792); t-ferulic acid (PubChem CD: 445858); o- coumaric acid (PubChem CD: 637540); (PubChem CD: quercetin 5280459); 4-hyroxybenzoic acid (PubChem CD: 135).

本研究利用响应面法(RSM)和梯度增强(GB)建模技术对热超声黑胡萝卜汁中生物活性成分进行了优化研究。热超声是超声和热的结合,用于提高黑胡萝卜汁的营养品质,黑胡萝卜汁富含花青素、酚类化合物和抗氧化剂。研究了温度、处理时间和超声振幅对总类胡萝卜素含量(TCC)、总花青素含量(TAC)、铁还原抗氧化能力(FRAP)和总酚含量的影响。与GB相比,RSM具有更高的预测精度,确定了最佳处理条件为48.68°C、11.15 min和82.62%的振幅。热超声处理显著提高总酚含量,达到414.28 mg GAE/L,超过传统的巴氏灭菌。通过模糊逻辑进行的感官分析表明,热超声样品的感官性能得到改善,包括香气、味道和颜色。本研究认为热超声是一种很有前途的方法,可以提高黑胡萝卜汁的生物活性成分和感官品质。化合物:Chlorogenıc酸(PubChem CD:1794427);咖啡酸(PubChem CD: 689043);香草醛(PubChem CD: 1183);芦丁(PubChem CD: 5280805);naringin (PubChem CD: 442428);迷迭香酸(PubChem CD: 5281792);t-阿魏酸(PubChem CD: 445858);o-香豆酸(PubChem CD: 637540);(PubChem CD: quercetin 5280459);4-羟基苯甲酸(PubChem CD: 135)
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引用次数: 0
Efficient voltammetric platform combining a molecularly imprinted polymer and silver-nanoparticle-decorated black phosphorus nanosheets for selective determination of Gatifloxacin. 结合分子印迹聚合物和纳米银修饰黑磷纳米片的高效伏安平台选择性测定加替沙星。
IF 6.5 1区 农林科学 Q1 CHEMISTRY, APPLIED Pub Date : 2024-12-14 eCollection Date: 2025-01-01 DOI: 10.1016/j.fochx.2024.102094
Jingtao Wu, Yonghui Xia, Tianyu Wang, Yafeng Zhang, Guangli Li

An ultrasensitive and selective voltammetric platform combined a molecularly imprinted poly(pyrrole) membrane with Ag-nanoparticle-functionalized black phosphorus nanosheets (MIP/BPNS-AgNPs) was developed for trace GAT detection. The physicochemical properties of the MIP/BPNS-AgNPs were studied by various spectroscopic and electrochemical techniques. BPNS-AgNPs improved the ambient stability and electrochemical activity of the BPNS and possessed a large surface area for accommodating abundant templates to produce specific imprinted sites. The resulting MIP/BPNS-AgNP-modified glassy carbon electrode (GCE) greatly enhanced voltammetric responses for GAT. The MIP/BPNS-AgNP/GCE exhibited admirable GAT determination performance, with two linear responses (0.001-1 and 1-50 μM), high sensitivity (9.965 and 0.5378 μA μM-1), and a low detection limit of 0.2 nM. In addition, the MIP electrode could selectively detect GAT in complex matrices and retain roust responses for a month. The applicability of MIP/BPNS-AgNP/GCE toward the detection of GAT in pharmaceutical formulations, milk, and human serum was verified with satisfactory results.

建立了一种结合分子印迹聚吡咯膜和ag纳米颗粒功能化黑磷纳米片(MIP/BPNS-AgNPs)的超灵敏选择性伏安检测平台,用于痕量GAT检测。利用各种光谱和电化学技术研究了MIP/BPNS-AgNPs的物理化学性质。BPNS- agnps提高了BPNS的环境稳定性和电化学活性,并且具有较大的表面积,可以容纳丰富的模板来产生特定的印迹位点。所得到的MIP/ bpns - agnp修饰的玻碳电极(GCE)大大提高了GAT的伏安响应。MIP/BPNS-AgNP/GCE具有良好的GAT测定性能,具有两个线性响应(0.001-1和1-50 μM),高灵敏度(9.965和0.5378 μA μM-1),低检出限0.2 nM。此外,MIP电极可以选择性地检测复杂基质中的GAT,并保持一个月的活跃反应。验证了MIP/BPNS-AgNP/GCE在制剂、牛奶和人血清中GAT检测中的适用性,结果令人满意。
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引用次数: 0
期刊
Food Chemistry: X
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